Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000923

Physical Location & Seq

Genomic Coordinates Forward (+)

41637558 .. 41641205

Gene Structure 3648 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1341300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2328 bp

ATGTGGTTTGGAAAGCTTTCGTTTTTGCTCCAGTTATTCGTTATATCTTCTCTGTTTCAAGTTCCCGCCTTTGCAATAAAAAAGTCGTATATAGTGTACCTGGGATCGCATGCACATGCTCCACAAGTCTCAGAAGCTGATCTTCATCGCGTCACTCATTCTCATCATGAATTCCTCGGATCATTCCTCGGAAGTACCGAGAAAGCGAAAGAAGCAATTATTTACTCGTACAAAAGACATATCAATGGTTTTGCTACCACCCTTGAAGAGGAAGAGGCGGCTCAAATCGCAAAGCATCCAAAAGTTGTGTCGGTTTTCTTAAACCGAGGAAGAAAATTGCACACTACTCACTCATGGGACTTTATGTTGCTTGAAAAGGACGGAGTAATCCACCCCAGTTCCTTGTGGAAAAAGGCCAGATTTGGTGAAGATACAATCATTGGGAATCTTGACACTGGTGTTTGGCCCGAATCAGAGAGCTTTAGAGACAAAGGGATTGGACCCGTTCCATCAAAGTGGAAAGGAATCTGCCAGAATCACACTGCCGGATTTGTTTGCAACAGGAAGCTTATAGGCACAAGGTACTTCAACAAAGGATATGCTGCATATGCAGGGGTACCAGTCAAGTCCTCCTTAAACAGTGCGCGAGACCATGAAGGTCATGGTTCTCACACTTTATCCACTGCAGGTGGCAACTTTGTTCCTGGAGCCAATGTGTTTGGTCTGGGGAATGGAACTGCCAAAGGTGGCTCTCCAAAAGCCCGTGTTGCTGCTTACAAAGTTTGCTGGCCACCCATCAATGGCAGTGAATGCTTTGATGCTGATATCATGGCAGCCTTTGATGCAGCCATACATGATGGGGTTGATGTGCTTTCAATCTCGCTTGGTGGAGACCCTTCCGATTACTTTAGTGATGGCGTTTCCATTGGTGCATTCCATGCTGTCAAGAATGGTATCGTTGTTGTATGCTCTGCAGGAAACTCTGGTCCTGCTGCTGGAACTGTCTCAAATGTTGCACCTTGGATGATAACCGTGGGAGCAAGTACTTTGGATCGAGAGTTTGAAACTATGATTGAACTCCGAAATGGGATGCGTCTCAAGGGAACGAGCCTTTCAAAGCCTTTACCAGATGACATATTTTATCCACTTATAACCGGTGCACAAGCTAAAGCAGATAATGCTTCTGCTAAGGATGCCATGCTTTGTTTAGGAGGATCGTTGGACCCAAAGAAGGTGAAGGGTAAGATTTTGGCCTGTCTTAGGGGACTAAGTGCAAGAGTAGACAAGGGTCAACAAGCATCACTAGCTGGAGCAGCAGGGATGATTCTTTGCAATGATGAGGCCAGTGGCAATGAACTCATCGCTGATCCTCACGTATTACCAGCATCCCAAATCAATTATACAGATGGCATTGCGGTCTTTAACTACATCAATTCTACAGACGATCCACTTGGATTTATAACGCCCCCAACTGCAAAGCTGAACACAAAACCTGCTCCATTCATGGCTTCATTCTCCTCTCAGGGCCCCAACACTATCACTCCTGAGATCCTCAAGCCTGATATCACCGCTCCTGGTGTAAATATCATAGCTGCCTACACCCAAGCAACCAGCCCTACAGATCAAGACTTTGATAAGCGCAGGGTTCCGTTTAACACGGAATCTGGCACCTCCATGTCTTGCCCTCATGTTTCTGGAGTCGTAGGACTTCTCAAGACACTCTACCCTCACTGGAGTCCATCTGCAATTAGATCAGCAATCATGACAACCGCAAGAACGAGGGACAACACTGTGAATCCAATGATGAGTGCATCATTTGTTGAGGCAACGCCATTCAGCTATGGCTCTGGCCATATACGACCAAATCGTGCCATGGATCCTGGCCTGATCTATGACCTAACTGCCAATGATTACTTGGACTTTCTTTGTGCCATTGGCTATAACAAGACAACGATTCAACTCTTTTCTGAGGGTCCTAACTACAAATGCCCCGAGTCAGCCAGTCTTCTGGACTTCAATTACCCTTCGATATCAGTCCCTCGATTGTCTGGTTCAGTCACTGTGACTAGAAGACTCAAGAATGTTGGTTCTCCGGGTACCTATGCAGTCCGTATTAAACGAGCAAATGGAATTTCAGTGAGTGTTGAGCCTAATGTCTTGAGGTTTAAAAGTGTTGGGGAAGAGAAGAGCTTTAGGGTGACACTGAAAGCGAAGCCTAAGAGAGGTGGAGTAGCTAAGGGGGATTATGTATTTGGAGGGCTTACCTGGTCGGATGGCAAGCACTACGTCAGGAGTCCAATCGTAGTTGCCTCAGCAGCTGCTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009888 GO:0010073 GO:0010074 GO:0016787 GO:0017171 GO:0019538 GO:0019827 GO:0032501 GO:0032502 GO:0043170 GO:0044238 GO:0048507 GO:0048856 GO:0070011 GO:0071704 GO:0098727 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

776

Amino Acids

83.04

Weight (kDa)

8.96

Isoelectric Point (pI)

32.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	29 - 114	2.2e-19	Peptidase inhibitor I9
Peptidase_S8	PF00082	142 - 599	5.4e-43	Subtilase family
PA	PF02225	397 - 471	1e-11	PA domain
fn3_6	PF17766	671 - 769	2e-29	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000923)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G59810
fragaria_vesca	FvH4_3g00161 FvH4_3g42040 FvH4_3g42040 FvH4_3g42040 FvH4_3g42040 FvH4_3g42040 FvH4_3g42040 FvH4_3g42040 FvH4_3g42041 FvH4_3g42350
malus_domestica	MD03G1039300.v1.1 MD10G1341300.v1.1 MD11G1039600.v1.1 MD14G1139100.v1.1
prunus_persica	Prupe.4G001800_v2.0.a1 Prupe.6G031400_v2.0.a1 Prupe.6G031500_v2.0.a1 Prupe.6G031500_v2.0.a1 Prupe.6G031500_v2.0.a1 Prupe.6G031500_v2.0.a1 Prupe.6G031500_v2.0.a1 Prupe.6G031500_v2.0.a1
pyrus_communis	pycom03g03030 pycom11g03350 pycom11g03360 pycom11g03370 pycom17g11100
rosa_chinensis	RchiOBHm_Chr5g0000221 RchiOBHm_Chr5g0075241 RchiOBHm_Chr5g0075251
rosa_laevigata	RLG00000030851 RLG00000036541 RLG00000036542
rosa_multiflora	Rmu_co8057730.1_g000001 Rmu_co8132088.1_g000001 Rmu_sc0000770.1_g000063 Rmu_sc0021936.1_g000001 Rmu_sc0031527.1_g000001 Rmu_ssc0000243.1_g000009
rosa_roxburghii	Rroxscaffold_1G00006310 Rroxscaffold_1G00075880 Rroxscaffold_3G00267190
rosa_rugosa	Rorug04G0382600 Rorug05G0437400 Rorug05G0437500 Rorug05G0437600 Rorug05G0437700 Rorug05G0437800
rosa_samantha	Rh5AG001500 Rh5AG494500 Rh5AG494600 Rh5BG002900 Rh5BG516100 Rh5BG516200 Rh5CG001800 Rh5CG539900 Rh5CG540000 Rh5DG001700 Rh5DG528800 Rh5DG528900
rosa_wichuraiana	Rw5G000200 Rw5G045880 Rw5G045890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1151, 1460
AarI	CACCTGC	1 cut(s)	677
Acc36I	ACCTGC	2 cut(s)	677, 1501
Acc65I	GGTACC	2 cut(s)	616, 2096
AccB1I	GGYRCC	3 cut(s)	616, 1667, 2096
AccBSI	CCGCTC	1 cut(s)	1571
AccI	GTMKAC	1 cut(s)	1281
AccII	CGCG	2 cut(s)	150, 646
AciI	CCGC	5 cut(s)	66, 278, 1415, 1569, 1770
AclWI	GGATC	9 cut(s)	112, 187, 1059, 1222, 1361, 1439, 1543, 1871, 1884
AcoI	YGGCCR	2 cut(s)	788, 1849
AcsI	RAATTY	2 cut(s)	170, 2130
AfaI	GTAC	7 cut(s)	98, 196, 230, 584, 618, 1045, 2098
AfiI	CCNNNNNNNGG	6 cut(s)	268, 800, 995, 1231, 1260, 2222
AgeI	ACCGGT	1 cut(s)	1154
AjnI	CCWGG	5 cut(s)	99, 703, 1573, 1879, 2264
Alw21I	GWGCWC	1 cut(s)	1162
Alw26I	GTCTC	6 cut(s)	133, 480, 642, 885, 1009, 1100
Alw44I	GTGCAC	1 cut(s)	1158
AlwI	GGATC	9 cut(s)	112, 187, 1059, 1222, 1361, 1439, 1543, 1871, 1884
AlwNI	CAGNNNCTG	4 cut(s)	137, 2060, 2318, 2321
Ama87I	CYCGRG	1 cut(s)	1991
AoxI	GGCC	8 cut(s)	414, 464, 788, 1251, 1341, 1525, 1849, 1882
ApaI	GGGCCC	1 cut(s)	1529
ApaLI	GTGCAC	1 cut(s)	1158
ApeKI	GCWGC	9 cut(s)	602, 770, 833, 845, 992, 1313, 1592, 2315, 2318
ApoI	RAATTY	2 cut(s)	170, 2130
ArsI	GACNNNNNNTTYG	2 cut(s)	293, 325
AsiGI	ACCGGT	1 cut(s)	1154
Asp700I	GAANNNNTTC	1 cut(s)	16
Asp718I	GGTACC	2 cut(s)	616, 2096
AspLEI	GCGC	2 cut(s)	646, 1641
AspS9I	GGNCC	7 cut(s)	465, 500, 986, 1222, 1525, 1526, 1973
AsuC2I	CCSGG	1 cut(s)	2094
AsuHPI	GGTGA	4 cut(s)	437, 1246, 1558, 2209
AvaI	CYCGRG	1 cut(s)	1991
AvaII	GGWCC	4 cut(s)	500, 986, 1222, 1973
BaeGI	GKGCMC	2 cut(s)	1162, 1529
BalI	TGGCCA	2 cut(s)	790, 1851
BamHI	GGATCC	1 cut(s)	1876
BanI	GGYRCC	3 cut(s)	616, 1667, 2096
BanII	GRGCYC	1 cut(s)	1529
BbsI	GAAGAC	2 cut(s)	1997, 2077
Bbv12I	GWGCWC	1 cut(s)	1162
BbvCI	CCTCAGC	1 cut(s)	2311
BbvI	GCAGC	8 cut(s)	589, 757, 845, 857, 979, 1325, 1579, 2305
BccI	CCATC	7 cut(s)	517, 803, 851, 908, 1400, 1747, 2267
BciT130I	CCWGG	5 cut(s)	101, 705, 1575, 1881, 2266
BcnI	CCSGG	1 cut(s)	2094
BcoDI	GTCTC	6 cut(s)	133, 480, 642, 885, 1009, 1100
BfaI	CTAG	2 cut(s)	1304, 2067
BfmI	CTRYAG	4 cut(s)	684, 972, 1437, 1617
BfuAI	ACCTGC	2 cut(s)	677, 1501
BmcAI	AGTACT	1 cut(s)	1045
Bme1390I	CCNGG	6 cut(s)	101, 705, 1575, 1881, 2094, 2266
Bme18I	GGWCC	4 cut(s)	500, 986, 1222, 1973
BmeT110I	CYCGRG	1 cut(s)	1991
BmgT120I	GGNCC	7 cut(s)	465, 500, 986, 1222, 1525, 1526, 1973
BmrFI	CCNGG	6 cut(s)	101, 705, 1575, 1881, 2094, 2266
BmrI	ACTGGG	1 cut(s)	390
BmsI	GCATC	8 cut(s)	304, 808, 832, 1080, 1183, 1307, 1394, 1820
BmuI	ACTGGG	1 cut(s)	390
BoxI	GACNNNNGTC	1 cut(s)	1287
BpiI	GAAGAC	2 cut(s)	1997, 2077
BpmI	CTGGAG	5 cut(s)	14, 726, 1329, 1716, 1753
Bpu10I	CCTNAGC	3 cut(s)	1188, 2235, 2311
BpuEI	CTTGAG	5 cut(s)	1082, 1538, 1697, 2060, 2179
BpuMI	CCSGG	1 cut(s)	2094
BsaAI	YACGTR	1 cut(s)	1375
BsaBI	GATNNNNATC	1 cut(s)	144
BsaI	GGTCTC	2 cut(s)	642, 885
BsaJI	CCNNGG	7 cut(s)	100, 175, 187, 325, 1019, 1032, 1872
BsaWI	WCCGGW	1 cut(s)	1154
BsaXI	ACNNNNNCTCC	4 cut(s)	699, 729, 1525, 1555
Bsc4I	CCNNNNNNNGG	6 cut(s)	268, 800, 995, 1231, 1260, 2222
Bse118I	RCCGGY	1 cut(s)	1154
Bse1I	ACTGG	7 cut(s)	31, 396, 460, 620, 1344, 1736, 2001
Bse3DI	GCAATG	3 cut(s)	1339, 1357, 1410
Bse8I	GATNNNNATC	1 cut(s)	144
BseBI	CCWGG	5 cut(s)	101, 705, 1575, 1881, 2266
BseDI	CCNNGG	7 cut(s)	100, 175, 187, 325, 1019, 1032, 1872
BseGI	GGATG	7 cut(s)	295, 1029, 1095, 1198, 1326, 1385, 2278
BseJI	GATNNNNATC	1 cut(s)	144
BseLI	CCNNNNNNNGG	6 cut(s)	268, 800, 995, 1231, 1260, 2222
BseMI	GCAATG	3 cut(s)	1339, 1357, 1410
BseMII	CTCAG	5 cut(s)	144, 1535, 1536, 1959, 2325
BseNI	ACTGG	7 cut(s)	31, 396, 460, 620, 1344, 1736, 2001
BseRI	GAGGAG	1 cut(s)	1507
BseSI	GKGCMC	2 cut(s)	1162, 1529
BseXI	GCAGC	8 cut(s)	589, 757, 845, 857, 979, 1325, 1579, 2305
Bsh1236I	CGCG	2 cut(s)	150, 646
BshFI	GGCC	8 cut(s)	416, 466, 790, 1253, 1343, 1527, 1851, 1884
BshNI	GGYRCC	3 cut(s)	616, 1667, 2096
BshTI	ACCGGT	1 cut(s)	1154
BsiHKAI	GWGCWC	1 cut(s)	1162
BsiHKCI	CYCGRG	1 cut(s)	1991
BsiSI	CCGG	3 cut(s)	546, 1155, 2093
BslFI	GGGAC	4 cut(s)	371, 1278, 1796, 2021
BslI	CCNNNNNNNGG	6 cut(s)	268, 800, 995, 1231, 1260, 2222
BsmAI	GTCTC	6 cut(s)	133, 480, 642, 885, 1009, 1100
BsmBI	CGTCTC	1 cut(s)	1100
BsmFI	GGGAC	4 cut(s)	371, 1278, 1796, 2021
BsmI	GAATGC	2 cut(s)	815, 932
BsnI	GGCC	8 cut(s)	416, 466, 790, 1253, 1343, 1527, 1851, 1884
Bso31I	GGTCTC	2 cut(s)	642, 885
BsoBI	CYCGRG	1 cut(s)	1991
Bsp120I	GGGCCC	1 cut(s)	1525
Bsp1286I	GDGCHC	2 cut(s)	1162, 1529
Bsp19I	CCATGG	1 cut(s)	1872
BspACI	CCGC	5 cut(s)	66, 278, 1415, 1569, 1770
BspANI	GGCC	8 cut(s)	416, 466, 790, 1253, 1343, 1527, 1851, 1884
BspCNI	CTCAG	5 cut(s)	143, 1534, 1537, 1960, 2324
BspFNI	CGCG	2 cut(s)	150, 646
BspHI	TCATGA	2 cut(s)	166, 1761
BspMAI	CTGCAG	2 cut(s)	688, 976
BspMI	ACCTGC	2 cut(s)	677, 1501
BspPI	GGATC	9 cut(s)	112, 187, 1059, 1222, 1361, 1439, 1543, 1871, 1884
BspQI	GCTCTTC	1 cut(s)	2180
BspT107I	GGYRCC	3 cut(s)	616, 1667, 2096
BspTNI	GGTCTC	2 cut(s)	642, 885
BsrBI	CCGCTC	1 cut(s)	1571
BsrDI	GCAATG	3 cut(s)	1339, 1357, 1410
BsrFI	RCCGGY	1 cut(s)	1154
BsrI	ACTGG	7 cut(s)	31, 396, 460, 620, 1344, 1736, 2001
BssAI	RCCGGY	1 cut(s)	1154
BssECI	CCNNGG	7 cut(s)	100, 175, 187, 325, 1019, 1032, 1872
BssT1I	CCWWGG	2 cut(s)	1019, 1872
Bst2UI	CCWGG	5 cut(s)	101, 705, 1575, 1881, 2266
Bst4CI	ACNGT	5 cut(s)	641, 1003, 1033, 1792, 2062
Bst6I	CTCTTC	4 cut(s)	261, 267, 2175, 2180
BstAPI	GCANNNNNTGC	3 cut(s)	810, 938, 1178
BstBAI	YACGTR	1 cut(s)	1375
BstC8I	GCNNGC	3 cut(s)	111, 788, 2279
BstDSI	CCRYGG	2 cut(s)	1032, 1872
BstENI	CCTNNNNNAGG	3 cut(s)	266, 1258, 2220
BstF5I	GGATG	7 cut(s)	295, 1029, 1095, 1198, 1326, 1385, 2278
BstFNI	CGCG	2 cut(s)	150, 646
BstHHI	GCGC	2 cut(s)	646, 1641
BstMAI	GTCTC	6 cut(s)	133, 480, 642, 885, 1009, 1100
BstNI	CCWGG	5 cut(s)	101, 705, 1575, 1881, 2266
BstNSI	RCATGY	2 cut(s)	113, 119
BstPAI	GACNNNNGTC	1 cut(s)	1287
BstSCI	CCNGG	6 cut(s)	99, 703, 1573, 1879, 2092, 2264
BstSFI	CTRYAG	4 cut(s)	684, 972, 1437, 1617
BstSLI	GKGCMC	2 cut(s)	1162, 1529
BstUI	CGCG	2 cut(s)	150, 646
BstV1I	GCAGC	8 cut(s)	589, 757, 845, 857, 979, 1325, 1579, 2305
BstV2I	GAAGAC	2 cut(s)	1997, 2077
BstX2I	RGATCY	2 cut(s)	1548, 1876
BstXI	CCANNNNNNTGG	2 cut(s)	516, 2008
BstYI	RGATCY	2 cut(s)	1548, 1876
BsuRI	GGCC	8 cut(s)	416, 466, 790, 1253, 1343, 1527, 1851, 1884
BtgI	CCRYGG	2 cut(s)	1032, 1872
BtgZI	GCGATG	2 cut(s)	131, 1345
BtsCI	GGATG	7 cut(s)	295, 1029, 1095, 1198, 1326, 1385, 2278
BtsI	GCAGTG	3 cut(s)	540, 681, 811
BveI	ACCTGC	2 cut(s)	677, 1501
Cac8I	GCNNGC	3 cut(s)	111, 788, 2279
CaiI	CAGNNNCTG	4 cut(s)	137, 2060, 2318, 2321
CciI	TCATGA	2 cut(s)	166, 1761
CfoI	GCGC	2 cut(s)	646, 1641
Cfr10I	RCCGGY	1 cut(s)	1154
Cfr13I	GGNCC	7 cut(s)	465, 500, 986, 1222, 1525, 1526, 1973
CseI	GACGC	2 cut(s)	139, 1082
CsiI	ACCWGGT	1 cut(s)	2264
Csp6I	GTAC	7 cut(s)	97, 195, 229, 583, 617, 1044, 2097
CspAI	ACCGGT	1 cut(s)	1154
CviQI	GTAC	7 cut(s)	97, 195, 229, 583, 617, 1044, 2097
DraI	TTTAAA	1 cut(s)	2167
EaeI	YGGCCR	2 cut(s)	788, 1849
Eam1104I	CTCTTC	4 cut(s)	261, 267, 2175, 2180
EarI	CTCTTC	4 cut(s)	261, 267, 2175, 2180
Eco130I	CCWWGG	2 cut(s)	1019, 1872
Eco24I	GRGCYC	1 cut(s)	1529
Eco31I	GGTCTC	2 cut(s)	642, 885
Eco32I	GATATC	3 cut(s)	826, 1564, 2031
Eco47I	GGWCC	4 cut(s)	500, 986, 1222, 1973
Eco88I	CYCGRG	1 cut(s)	1991
EcoNI	CCTNNNNNAGG	3 cut(s)	266, 1258, 2220
EcoO109I	RGGNCCY	3 cut(s)	1525, 1526, 1973
EcoRI	GAATTC	1 cut(s)	170
EcoRII	CCWGG	5 cut(s)	99, 703, 1573, 1879, 2264
EcoRV	GATATC	3 cut(s)	826, 1564, 2031
EcoT14I	CCWWGG	2 cut(s)	1019, 1872
EcoT38I	GRGCYC	1 cut(s)	1529
ErhI	CCWWGG	2 cut(s)	1019, 1872
Esp3I	CGTCTC	1 cut(s)	1100
FalI	AAGNNNNNCTT	4 cut(s)	126, 158, 617, 649
FaqI	GGGAC	4 cut(s)	371, 1278, 1796, 2021
FauI	CCCGC	1 cut(s)	73
FauNDI	CATATG	1 cut(s)	607
FblI	GTMKAC	1 cut(s)	1281
FokI	GGATG	7 cut(s)	282, 1036, 1102, 1205, 1333, 1372, 2285
FriOI	GRGCYC	1 cut(s)	1529
FspBI	CTAG	2 cut(s)	1304, 2067
GlaI	GCGC	2 cut(s)	645, 1640
GsuI	CTGGAG	5 cut(s)	14, 726, 1329, 1716, 1753
HaeIII	GGCC	8 cut(s)	416, 466, 790, 1253, 1343, 1527, 1851, 1884
HapII	CCGG	3 cut(s)	546, 1155, 2093
HgaI	GACGC	2 cut(s)	139, 1082
HhaI	GCGC	2 cut(s)	646, 1641
Hin6I	GCGC	2 cut(s)	644, 1639
HinP1I	GCGC	2 cut(s)	644, 1639
HincII	GTYRAC	1 cut(s)	1292
HindII	GTYRAC	1 cut(s)	1292
HindIII	AAGCTT	2 cut(s)	14, 566
HpaII	CCGG	3 cut(s)	546, 1155, 2093
HphI	GGTGA	4 cut(s)	437, 1246, 1558, 2209
Hpy166II	GTNNAC	4 cut(s)	97, 1160, 1282, 1292
Hpy188I	TCNGA	8 cut(s)	133, 179, 191, 475, 901, 1082, 1969, 2272
Hpy8I	GTNNAC	4 cut(s)	97, 1160, 1282, 1292
HpyAV	CCTTC	5 cut(s)	650, 906, 1225, 1231, 2034
HpyCH4III	ACNGT	5 cut(s)	641, 1003, 1033, 1792, 2062
HpyCH4IV	ACGT	2 cut(s)	1374, 2286
HpySE526I	ACGT	2 cut(s)	1374, 2286
HspAI	GCGC	2 cut(s)	644, 1639
KpnI	GGTACC	2 cut(s)	620, 2100
LguI	GCTCTTC	1 cut(s)	2180
LmnI	GCTCC	7 cut(s)	33, 124, 707, 1037, 1310, 1501, 1576
Lsp1109I	GCAGC	8 cut(s)	589, 757, 845, 857, 979, 1325, 1579, 2305
LweI	GCATC	8 cut(s)	304, 808, 832, 1080, 1183, 1307, 1394, 1820
MabI	ACCWGGT	1 cut(s)	2264
MaeI	CTAG	2 cut(s)	1304, 2067
MaeII	ACGT	2 cut(s)	1374, 2286
MaeIII	GTNAC	4 cut(s)	151, 2056, 2062, 2197
MbiI	CCGCTC	1 cut(s)	1571
MflI	RGATCY	2 cut(s)	1548, 1876
MhlI	GDGCHC	2 cut(s)	1162, 1529
MlsI	TGGCCA	2 cut(s)	790, 1851
MluCI	AATT	8 cut(s)	170, 216, 335, 1396, 1432, 1746, 2017, 2130
MluNI	TGGCCA	2 cut(s)	790, 1851
MlyI	GAGTC	5 cut(s)	1707, 1744, 2003, 2067, 2302
MmeI	TCCRAC	2 cut(s)	1200, 2250
Mox20I	TGGCCA	2 cut(s)	790, 1851
MroXI	GAANNNNTTC	1 cut(s)	16
MscI	TGGCCA	2 cut(s)	790, 1851
MseI	TTAA	6 cut(s)	320, 635, 1422, 1653, 2115, 2166
MslI	CAYNNNNRTG	4 cut(s)	114, 243, 514, 1673
Msp20I	TGGCCA	2 cut(s)	790, 1851
MspA1I	CMGCKG	1 cut(s)	2318
MspI	CCGG	3 cut(s)	546, 1155, 2093
MspR9I	CCNGG	6 cut(s)	101, 705, 1575, 1881, 2094, 2266
Mva1269I	GAATGC	2 cut(s)	815, 932
MvaI	CCWGG	5 cut(s)	101, 705, 1575, 1881, 2266
MvnI	CGCG	2 cut(s)	150, 646
NciI	CCSGG	1 cut(s)	2094
NcoI	CCATGG	1 cut(s)	1872
NdeI	CATATG	1 cut(s)	607
NmuCI	GTSAC	4 cut(s)	151, 2056, 2062, 2197
NspI	RCATGY	2 cut(s)	113, 119
PaeI	GCATGC	1 cut(s)	113
PagI	TCATGA	2 cut(s)	166, 1761
PaqCI	CACCTGC	1 cut(s)	677
PciSI	GCTCTTC	1 cut(s)	2180
PcsI	WCGNNNNNNNCGW	2 cut(s)	195, 1864
PctI	GAATGC	2 cut(s)	815, 932
PdmI	GAANNNNTTC	1 cut(s)	16
PfeI	GAWTC	8 cut(s)	445, 470, 525, 535, 1324, 1661, 1795, 1954
PfoI	TCCNGGA	1 cut(s)	703
PinAI	ACCGGT	1 cut(s)	1154
PleI	GAGTC	5 cut(s)	1706, 1743, 2002, 2067, 2301
PpsI	GAGTC	5 cut(s)	1706, 1743, 2002, 2067, 2301
Ppu21I	YACGTR	1 cut(s)	1375
PpuMI	RGGWCCY	1 cut(s)	1973
PshAI	GACNNNNGTC	1 cut(s)	1287
PsiI	TTATAA	2 cut(s)	1151, 1460
Psp5II	RGGWCCY	1 cut(s)	1973
Psp6I	CCWGG	5 cut(s)	99, 703, 1573, 1879, 2264
PspGI	CCWGG	5 cut(s)	99, 703, 1573, 1879, 2264
PspOMI	GGGCCC	1 cut(s)	1525
PspPI	GGNCC	7 cut(s)	465, 500, 986, 1222, 1525, 1526, 1973
PspPPI	RGGWCCY	1 cut(s)	1973
PstI	CTGCAG	2 cut(s)	688, 976
PstNI	CAGNNNCTG	4 cut(s)	137, 2060, 2318, 2321
PsuI	RGATCY	2 cut(s)	1548, 1876
PvuII	CAGCTG	1 cut(s)	2318
RsaI	GTAC	7 cut(s)	98, 196, 230, 584, 618, 1045, 2098
RsaNI	GTAC	7 cut(s)	97, 195, 229, 583, 617, 1044, 2097
RseI	CAYNNNNRTG	4 cut(s)	114, 243, 514, 1673
SapI	GCTCTTC	1 cut(s)	2180
SaqAI	TTAA	6 cut(s)	320, 635, 1422, 1653, 2115, 2166
Sau96I	GGNCC	7 cut(s)	465, 500, 986, 1222, 1525, 1526, 1973
ScaI	AGTACT	1 cut(s)	1045
SchI	GAGTC	5 cut(s)	1707, 1744, 2003, 2067, 2302
ScrFI	CCNGG	6 cut(s)	101, 705, 1575, 1881, 2094, 2266
SduI	GDGCHC	2 cut(s)	1162, 1529
SexAI	ACCWGGT	1 cut(s)	2264
SfaNI	GCATC	8 cut(s)	304, 808, 832, 1080, 1183, 1307, 1394, 1820
SfcI	CTRYAG	4 cut(s)	684, 972, 1437, 1617
SinI	GGWCC	4 cut(s)	500, 986, 1222, 1973
SmiMI	CAYNNNNRTG	4 cut(s)	114, 243, 514, 1673
SmlI	CTYRAG	5 cut(s)	1097, 1553, 1712, 2075, 2158
SmoI	CTYRAG	5 cut(s)	1097, 1553, 1712, 2075, 2158
SphI	GCATGC	1 cut(s)	113
Sse9I	AATT	8 cut(s)	170, 216, 335, 1396, 1432, 1746, 2017, 2130
SsiI	CCGC	5 cut(s)	66, 278, 1415, 1569, 1770
SspMI	CTAG	2 cut(s)	1304, 2067
StyD4I	CCNGG	6 cut(s)	99, 703, 1573, 1879, 2092, 2264
StyI	CCWWGG	2 cut(s)	1019, 1872
TaaI	ACNGT	5 cut(s)	641, 1003, 1033, 1792, 2062
TaiI	ACGT	2 cut(s)	1377, 2289
TaqI	TCGA	3 cut(s)	1054, 2027, 2041
TasI	AATT	8 cut(s)	170, 216, 335, 1396, 1432, 1746, 2017, 2130
TatI	WGTACW	1 cut(s)	1043
TauI	GCSGC	1 cut(s)	281
TfiI	GAWTC	8 cut(s)	445, 470, 525, 535, 1324, 1661, 1795, 1954
Tru1I	TTAA	6 cut(s)	320, 635, 1422, 1653, 2115, 2166
Tru9I	TTAA	6 cut(s)	320, 635, 1422, 1653, 2115, 2166
TseFI	GTSAC	4 cut(s)	151, 2056, 2062, 2197
TseI	GCWGC	9 cut(s)	602, 770, 833, 845, 992, 1313, 1592, 2315, 2318
Tsp45I	GTSAC	4 cut(s)	151, 2056, 2062, 2197
TspDTI	ATGAA	6 cut(s)	134, 183, 669, 1368, 1492, 1500
TspGWI	ACGGA	4 cut(s)	396, 1638, 1673, 2099
VneI	GTGCAC	1 cut(s)	1158
VpaK11BI	GGWCC	4 cut(s)	500, 986, 1222, 1973
XagI	CCTNNNNNAGG	3 cut(s)	266, 1258, 2220
XapI	RAATTY	2 cut(s)	170, 2130
XceI	RCATGY	2 cut(s)	113, 119
XcmI	CCANNNNNNNNNTGG	2 cut(s)	659, 1912
XmiI	GTMKAC	1 cut(s)	1281
XmnI	GAANNNNTTC	1 cut(s)	16
XspI	CTAG	2 cut(s)	1304, 2067
ZrmI	AGTACT	1 cut(s)	1045

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.