Rw5G045890

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000923
Physical Location & Seq
Reverse (-)
79899729 .. 79903342
3614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045890.1

Sequence Viewer

Length: 2376 bp
ATGGGGCATTGTTCACGTCTCCCTCTTTTTCTTCTATCTCTTCTTCTCCTCTCTCTGTTTCAGACGCCAGCCGATGCAGCCAAACAGGCATGCCTTAACAGAATTATGAATTTGAGATTGTCTTACATTGTGTACTTGGGAACACATTCCCATGGCTTGAACCCTACGTCTGTCGAACTCGAAGCGGCAACGAATTCTCAGTACAATCTGCTTGGAACTGTTTTGGGAAGCAATGAGAGGGCCCAAGAAGCATTGTTTTACTCATATACTAGAGAGATCAATGGATTTGCTGCAGTTTTAGAAGAAGAAGAGGCTTCACAGATTGCAAAGGATCCAAGTGTTGTGTCTGTTTTTCCAAACAGAAAATTAAAACTACATACAACTCATTCATGGGAGTTCCTGGGACTAGAAGACCAAAATGGAGTACCTCGTCACGGATCGATTTGGAAGAAAGCTAAGTTTGGTGAAAATACAATCATTGGAAACCTTGACACTGGTGTCTGGCCGGAATCACCGAGCTTTAGCGATGAAGGGTTTGGACCAATCCCATCCAAGTGGCGTGGCGCTTGTCAGCTTGACACCAAAGACAAAGTTCGTTGCAACAGGAAGCTGATCGGAGCAAGGTACTTTAACAAAGGCTTCTTGGAGTCTGCCAGGCTCAATAATGCCACCATTCCACCAGAAATTCTGGTGTCACCGCGTGACCAGGAAGGCCATGGCTCACACACCCTTTCAACAGCTGGTGGCATTTATGTGCCGGGGGCTAATGTTTTTGGTCATGGCAATGGCACTGCTAAAGGTGGATCCCCAAAAGCTCGCGTGGCTGCTTACAAAGTATGTTGGCCAAGCTTTGGCAATACAGAAGGAGGGTGTACTAATGCAGACATCATGGCCGCCTTTGATGCTGCAATCAGTGACGGTGTTGATGTTCTTTCGCTGTCCGTTGGTGGTGATTCTGTAGAGTTTTTTGAGGATGGGATCGCAATAGGGTCATTCCATGCGGCTAAGCATGGAATCGTTGTTGTTTGCTCAGCTGGCAATTCAGGGCCTACCCAAGGGACAGTGACTAATGTGGCACCCTGGATATTAACAGTTGGAGCTAGCACGATTGATCGCGAGTTCACTAGTTATTTATCCTTTGGAAAGGGGAAGCATTTCAAGGGAAAAAGTCTTTCTTCCAGAAGTTTTCCATCTAAGAAGTTTTATCCTGTGATAAGTGGAGCCAATGCTAATGCAGCTACTTCGCCTCCTGAAGATTCCTTGCTATGTTTGAATGGAACCCTTGATCCAAAGAAGGTTAAGGGGAAAATCTTGGTCTGCCTCCGCGGGCAGAATGGGAGAACTGAAAAGGCCAATATAGCTGCTCTTGCTGGCGCTGTTGGAATGGTTTTGGTTAACGATGAGCAAAGTGGAGATGATCTTGTGGCTGATCCTTATCAGATCCCCGCTACACATCTGACTTACACAGATGGAAAACGTGTCTTTGAGTACCTGAATTCTACCAAGAACCCTTTTGGTCACGTTACTCCTGTCAAGACGGTAGAAGGAACAAAGCCAGCTCCATTTATGGCTTCATTCTCATCTCGTGGACCGAGTACCATTGAGCCATCAATCCTTAAGCCTGATATCACAGCACCCGGAGTGGATATAATTGCTGCTTATACTGGAGCACCTCTCGCAAGTGATTCGGACATTGGTAAGCGTCGCCCACTTTACATTACAGAATCAGGAACATCTATGTCATGCCCTCATGTATCTGGAATAGTCGGTCTTCTGAAAACACTTTACCCGTCATGGAGCCCCGCTGCTATTAAGTCTGCAATCTTGACCACAGCGAGAAGACGCGACAACAAACGGGAGTCAATTCTTGATTCAACCAAGGTCTCAGCTACACCACTTGACTATGGTGCAGGCCATGTTGATCCAAATCGCGCAGCAAACCCCGGACTTGTCTATGACTTGACTACCGATGATTACTTGAATTTCTTATGTGCTCGTGGATACAATGCATCACTTCTCAAAATATTTTCCGACAAGCCACACAAATGTTCCAACACCTCTAGCATTATTGCTGATTTAAACTATCCTACAATTGCTGTTCCTGATCTCCAAGACAAACCAATTACCATTTCTAGAAAAGTGAAGAATGTCGGTACTCCAGGCACATATGTAGCATATGTTCGTGCACCAGCTAGAGTTTCTGTTTCGGTTCAGCCTAGCAGGTTGAAATTCAAGTCCATTGGTGAAGAGAAGGAATTCAAGGTTACGCTGAAGGCAGATGGTAAGGTTCGAAATCCAGATTATGTGTTTGGAGAGTTGAAATGGTCGGATGGCAAGCACGCTGTGAGGAGTCCTATTGCTGTGATGCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

791

Amino Acids

84.93

Weight (kDa)

8.96

Isoelectric Point (pI)

31.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 41 - 126 6e-17 Peptidase inhibitor I9
MORF_dom PF21864 69 - 125 1.8e-06 Multiple organellar RNA editing factor 1, MORF domain
Peptidase_S8 PF00082 155 - 617 9.8e-40 Subtilase family
PA PF02225 418 - 493 6.3e-14 PA domain
fn3_6 PF17766 692 - 788 5.1e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 497
Acc36I ACCTGC 1 cut(s) 2211
AccB1I GGYRCC 1 cut(s) 1075
AccB7I CCANNNNNTGG 1 cut(s) 851
AccII CGCG 6 cut(s) 700, 819, 1116, 1326, 1845, 1932
AciI CCGC 8 cut(s) 185, 698, 894, 1001, 1324, 1326, 1446, 1803
AcoI YGGCCR 3 cut(s) 503, 842, 891
AcsI RAATTY 7 cut(s) 109, 193, 684, 1495, 1981, 2228, 2255
AcuI CTGAAG 2 cut(s) 1272, 2291
AcyI GRCGYC 1 cut(s) 65
AdeI CACNNNGTG 2 cut(s) 701, 2344
AfaI GTAC 8 cut(s) 134, 203, 426, 626, 874, 1490, 1597, 2155
AfiI CCNNNNNNNGG 3 cut(s) 434, 851, 1055
AflII CTTAAG 1 cut(s) 1616
AflIII ACRYGT 1 cut(s) 1477
AhlI ACTAGT 1 cut(s) 1124
AjiI CACGTC 1 cut(s) 17
AjnI CCWGG 5 cut(s) 399, 653, 705, 1079, 2158
AjuI GAANNNNNNNTTGG 2 cut(s) 2113, 2145
AloI GAACNNNNNNTCC 2 cut(s) 1270, 1302
Alw21I GWGCWC 3 cut(s) 1672, 1996, 2188
Alw26I GTCTC 2 cut(s) 23, 1888
Alw44I GTGCAC 1 cut(s) 2184
AoxI GGCC 8 cut(s) 240, 503, 712, 842, 891, 1046, 1350, 1912
ApaI GGGCCC 1 cut(s) 244
ApaLI GTGCAC 1 cut(s) 2184
ApeKI GCWGC 9 cut(s) 77, 290, 824, 905, 1235, 1361, 1655, 1805, 1934
ApoI RAATTY 7 cut(s) 109, 193, 684, 1495, 1981, 2228, 2255
Asp700I GAANNNNTTC 3 cut(s) 145, 1154, 2255
AspLEI GCGC 3 cut(s) 566, 1376, 1934
AspS9I GGNCC 5 cut(s) 240, 241, 539, 1046, 1589
AsuC2I CCSGG 3 cut(s) 759, 1638, 1944
AsuHPI GGTGA 5 cut(s) 476, 504, 687, 962, 2255
AsuII TTCGAA 1 cut(s) 2290
AsuNHI GCTAGC 1 cut(s) 1100
AvaII GGWCC 2 cut(s) 539, 1589
BaeGI GKGCMC 2 cut(s) 244, 2188
BalI TGGCCA 1 cut(s) 844
BamHI GGATCC 2 cut(s) 331, 803
BanI GGYRCC 1 cut(s) 1075
BanII GRGCYC 2 cut(s) 244, 1802
BauI CACGAG 2 cut(s) 1584, 1995
BbsI GAAGAC 3 cut(s) 417, 1763, 1846
Bbv12I GWGCWC 3 cut(s) 1672, 1996, 2188
BbvI GCAGC 9 cut(s) 89, 277, 811, 892, 1247, 1348, 1642, 1792, 1946
BccI CCATC 7 cut(s) 556, 968, 1198, 1463, 1615, 2273, 2324
BcgI CGANNNNNNTGC 4 cut(s) 1224, 1258, 1668, 1702
BciT130I CCWGG 5 cut(s) 401, 655, 707, 1081, 2160
BciVI GTATCC 1 cut(s) 1994
BcnI CCSGG 3 cut(s) 759, 1638, 1944
BcoDI GTCTC 2 cut(s) 23, 1888
BcuI ACTAGT 1 cut(s) 1124
BfaI CTAG 9 cut(s) 270, 407, 1101, 1125, 2061, 2133, 2193, 2217, 2374
BfmI CTRYAG 2 cut(s) 291, 957
BfoI RGCGCY 2 cut(s) 567, 1377
BfrI CTTAAG 1 cut(s) 1616
BfuAI ACCTGC 1 cut(s) 2211
BfuI GTATCC 1 cut(s) 1994
BglI GCCNNNNNGGC 1 cut(s) 86
BlpI GCTNAGC 2 cut(s) 1005, 1030
Bme1390I CCNGG 8 cut(s) 401, 655, 707, 759, 1081, 1638, 1944, 2160
Bme18I GGWCC 2 cut(s) 539, 1589
BmgBI CACGTC 1 cut(s) 17
BmgT120I GGNCC 5 cut(s) 240, 241, 539, 1046, 1589
BmiI GGNNCC 7 cut(s) 242, 333, 805, 1077, 1222, 1279, 1799
BmrFI CCNGG 8 cut(s) 401, 655, 707, 759, 1081, 1638, 1944, 2160
BmsI GCATC 4 cut(s) 64, 892, 2018, 2355
BmtI GCTAGC 1 cut(s) 1104
BpiI GAAGAC 3 cut(s) 417, 1763, 1846
BplI GAGNNNNNCTC 2 cut(s) 1659, 1691
BpmI CTGGAG 2 cut(s) 1686, 2142
Bpu1102I GCTNAGC 2 cut(s) 1005, 1030
Bpu14I TTCGAA 1 cut(s) 2290
BpuMI CCSGG 3 cut(s) 759, 1638, 1944
Bsa29I ATCGAT 1 cut(s) 440
BsaBI GATNNNNATC 2 cut(s) 1434, 1926
BsaHI GRCGYC 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 1888
BsaJI CCNNGG 9 cut(s) 151, 400, 715, 758, 1054, 1079, 1324, 1878, 1942
BsaXI ACNNNNNCTCC 2 cut(s) 1231, 1261
Bsc4I CCNNNNNNNGG 3 cut(s) 434, 851, 1055
Bse1I ACTGG 2 cut(s) 499, 1669
Bse3DI GCAATG 2 cut(s) 238, 790
Bse8I GATNNNNATC 2 cut(s) 1434, 1926
BseBI CCWGG 5 cut(s) 401, 655, 707, 1081, 2160
BseCI ATCGAT 1 cut(s) 440
BseDI CCNNGG 9 cut(s) 151, 400, 715, 758, 1054, 1079, 1324, 1878, 1942
BseGI GGATG 3 cut(s) 548, 979, 2335
BseJI GATNNNNATC 2 cut(s) 1434, 1926
BseLI CCNNNNNNNGG 3 cut(s) 434, 851, 1055
BseMI GCAATG 2 cut(s) 238, 790
BseMII CTCAG 3 cut(s) 212, 1044, 1899
BseNI ACTGG 2 cut(s) 499, 1669
BseRI GAGGAG 2 cut(s) 38, 2362
BseSI GKGCMC 2 cut(s) 244, 2188
BseXI GCAGC 9 cut(s) 89, 277, 811, 892, 1247, 1348, 1642, 1792, 1946
BsgI GTGCAG 1 cut(s) 1929
Bsh1236I CGCG 6 cut(s) 700, 819, 1116, 1326, 1845, 1932
BshFI GGCC 8 cut(s) 242, 505, 714, 844, 893, 1048, 1352, 1914
BshNI GGYRCC 1 cut(s) 1075
BshVI ATCGAT 1 cut(s) 440
BsiHKAI GWGCWC 3 cut(s) 1672, 1996, 2188
BsiSI CCGG 4 cut(s) 506, 758, 1638, 1944
BslFI GGGAC 2 cut(s) 417, 1072
BslI CCNNNNNNNGG 3 cut(s) 434, 851, 1055
BsmAI GTCTC 2 cut(s) 23, 1888
BsmBI CGTCTC 1 cut(s) 23
BsmFI GGGAC 2 cut(s) 417, 1072
BsnI GGCC 8 cut(s) 242, 505, 714, 844, 893, 1048, 1352, 1914
Bso31I GGTCTC 1 cut(s) 1888
Bsp119I TTCGAA 1 cut(s) 2290
Bsp120I GGGCCC 1 cut(s) 240
Bsp1286I GDGCHC 5 cut(s) 244, 1672, 1802, 1996, 2188
Bsp1720I GCTNAGC 2 cut(s) 1005, 1030
Bsp19I CCATGG 2 cut(s) 151, 715
Bsp68I TCGCGA 1 cut(s) 1116
BspACI CCGC 8 cut(s) 185, 698, 894, 1001, 1324, 1326, 1446, 1803
BspANI GGCC 8 cut(s) 242, 505, 714, 844, 893, 1048, 1352, 1914
BspCNI CTCAG 3 cut(s) 211, 1043, 1898
BspDI ATCGAT 1 cut(s) 440
BspFNI CGCG 6 cut(s) 700, 819, 1116, 1326, 1845, 1932
BspLI GGNNCC 7 cut(s) 242, 333, 805, 1077, 1222, 1279, 1799
BspMAI CTGCAG 1 cut(s) 295
BspMI ACCTGC 1 cut(s) 2211
BspOI GCTAGC 1 cut(s) 1104
BspT104I TTCGAA 1 cut(s) 2290
BspT107I GGYRCC 1 cut(s) 1075
BspTI CTTAAG 1 cut(s) 1616
BspTNI GGTCTC 1 cut(s) 1888
BsrDI GCAATG 2 cut(s) 238, 790
BsrI ACTGG 2 cut(s) 499, 1669
BssECI CCNNGG 9 cut(s) 151, 400, 715, 758, 1054, 1079, 1324, 1878, 1942
BssNI GRCGYC 1 cut(s) 65
BssSI CACGAG 2 cut(s) 1584, 1995
BssT1I CCWWGG 4 cut(s) 151, 715, 1054, 1878
Bst2BI CACGAG 2 cut(s) 1584, 1995
Bst2UI CCWGG 5 cut(s) 401, 655, 707, 1081, 2160
Bst4CI ACNGT 5 cut(s) 220, 920, 1063, 1093, 1540
Bst6I CTCTTC 3 cut(s) 45, 303, 2241
BstACI GRCGYC 1 cut(s) 65
BstAFI CTTAAG 1 cut(s) 1616
BstBI TTCGAA 1 cut(s) 2290
BstDEI CTNAG 6 cut(s) 198, 456, 1005, 1030, 1194, 1885
BstDSI CCRYGG 3 cut(s) 151, 715, 1324
BstENI CCTNNNNNAGG 1 cut(s) 1053
BstF5I GGATG 3 cut(s) 548, 979, 2335
BstFNI CGCG 6 cut(s) 700, 819, 1116, 1326, 1845, 1932
BstH2I RGCGCY 2 cut(s) 567, 1377
BstHHI GCGC 3 cut(s) 566, 1376, 1934
BstMAI GTCTC 2 cut(s) 23, 1888
BstNI CCWGG 5 cut(s) 401, 655, 707, 1081, 2160
BstNSI RCATGY 1 cut(s) 93
BstSCI CCNGG 8 cut(s) 399, 653, 705, 757, 1079, 1636, 1942, 2158
BstSFI CTRYAG 2 cut(s) 291, 957
BstSLI GKGCMC 2 cut(s) 244, 2188
BstUI CGCG 6 cut(s) 700, 819, 1116, 1326, 1845, 1932
BstV1I GCAGC 9 cut(s) 89, 277, 811, 892, 1247, 1348, 1642, 1792, 1946
BstV2I GAAGAC 3 cut(s) 417, 1763, 1846
BstX2I RGATCY 3 cut(s) 331, 803, 1440
BstXI CCANNNNNNTGG 1 cut(s) 555
BstYI RGATCY 3 cut(s) 331, 803, 1440
Bsu15I ATCGAT 1 cut(s) 440
BsuI GTATCC 1 cut(s) 1994
BsuRI GGCC 8 cut(s) 242, 505, 714, 844, 893, 1048, 1352, 1914
BsuTUI ATCGAT 1 cut(s) 440
BtgI CCRYGG 3 cut(s) 151, 715, 1324
BtgZI GCGATG 1 cut(s) 540
BtrI CACGTC 1 cut(s) 17
BtsCI GGATG 3 cut(s) 548, 979, 2335
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 4 cut(s) 492, 789, 919, 1068
BtuMI TCGCGA 1 cut(s) 1116
BveI ACCTGC 1 cut(s) 2211
CfoI GCGC 3 cut(s) 566, 1376, 1934
Cfr13I GGNCC 5 cut(s) 240, 241, 539, 1046, 1589
Cfr42I CCGCGG 1 cut(s) 1327
ClaI ATCGAT 1 cut(s) 440
CseI GACGC 4 cut(s) 73, 1691, 1851, 2357
Csp6I GTAC 8 cut(s) 133, 202, 425, 625, 873, 1489, 1596, 2154
CspCI CAANNNNNGTGG 2 cut(s) 541, 576
CviQI GTAC 8 cut(s) 133, 202, 425, 625, 873, 1489, 1596, 2154
DdeI CTNAG 6 cut(s) 198, 456, 1005, 1030, 1194, 1885
DraI TTTAAA 1 cut(s) 2079
DraIII CACNNNGTG 2 cut(s) 701, 2344
DrdI GACNNNNNNGTC 1 cut(s) 497
DseDI GACNNNNNNGTC 1 cut(s) 497
EaeI YGGCCR 3 cut(s) 503, 842, 891
Eam1104I CTCTTC 3 cut(s) 45, 303, 2241
EarI CTCTTC 3 cut(s) 45, 303, 2241
Eco130I CCWWGG 4 cut(s) 151, 715, 1054, 1878
Eco24I GRGCYC 2 cut(s) 244, 1802
Eco31I GGTCTC 1 cut(s) 1888
Eco32I GATATC 1 cut(s) 1627
Eco47I GGWCC 2 cut(s) 539, 1589
Eco57I CTGAAG 2 cut(s) 1272, 2291
EcoNI CCTNNNNNAGG 1 cut(s) 1053
EcoO109I RGGNCCY 2 cut(s) 240, 1046
EcoRI GAATTC 3 cut(s) 193, 1495, 2255
EcoRII CCWGG 5 cut(s) 399, 653, 705, 1079, 2158
EcoRV GATATC 1 cut(s) 1627
EcoT14I CCWWGG 4 cut(s) 151, 715, 1054, 1878
EcoT22I ATGCAT 1 cut(s) 2011
EcoT38I GRGCYC 2 cut(s) 244, 1802
ErhI CCWWGG 4 cut(s) 151, 715, 1054, 1878
Esp3I CGTCTC 1 cut(s) 23
FalI AAGNNNNNCTT 2 cut(s) 1159, 1191
FaqI GGGAC 2 cut(s) 417, 1072
FauI CCCGC 3 cut(s) 1319, 1453, 1810
FauNDI CATATG 2 cut(s) 2167, 2176
FokI GGATG 3 cut(s) 535, 986, 2342
FriOI GRGCYC 2 cut(s) 244, 1802
FspBI CTAG 9 cut(s) 270, 407, 1101, 1125, 2061, 2133, 2193, 2217, 2374
GlaI GCGC 3 cut(s) 565, 1375, 1933
GsuI CTGGAG 2 cut(s) 1686, 2142
HaeII RGCGCY 2 cut(s) 567, 1377
HaeIII GGCC 8 cut(s) 242, 505, 714, 844, 893, 1048, 1352, 1914
HapII CCGG 4 cut(s) 506, 758, 1638, 1944
HgaI GACGC 4 cut(s) 73, 1691, 1851, 2357
HhaI GCGC 3 cut(s) 566, 1376, 1934
Hin1I GRCGYC 1 cut(s) 65
Hin6I GCGC 3 cut(s) 564, 1374, 1932
HinP1I GCGC 3 cut(s) 564, 1374, 1932
HincII GTYRAC 1 cut(s) 1396
HindII GTYRAC 1 cut(s) 1396
HindIII AAGCTT 1 cut(s) 847
HpaI GTTAAC 1 cut(s) 1396
HpaII CCGG 4 cut(s) 506, 758, 1638, 1944
HphI GGTGA 5 cut(s) 476, 504, 687, 962, 2255
Hpy166II GTNNAC 7 cut(s) 14, 133, 873, 1122, 1396, 1589, 2186
Hpy188I TCNGA 8 cut(s) 63, 617, 1440, 1458, 1690, 1776, 2032, 2329
Hpy8I GTNNAC 7 cut(s) 14, 133, 873, 1122, 1396, 1589, 2186
Hpy99I CGWCG 1 cut(s) 1707
HpyAV CCTTC 7 cut(s) 524, 704, 857, 1288, 1538, 2245, 2266
HpyCH4III ACNGT 5 cut(s) 220, 920, 1063, 1093, 1540
HpyCH4IV ACGT 4 cut(s) 16, 167, 1477, 1521
HpyF3I CTNAG 6 cut(s) 198, 456, 1005, 1030, 1194, 1885
HpySE526I ACGT 4 cut(s) 16, 167, 1477, 1521
Hsp92I GRCGYC 1 cut(s) 65
HspAI GCGC 3 cut(s) 564, 1374, 1932
KspAI GTTAAC 1 cut(s) 1396
KspI CCGCGG 1 cut(s) 1327
LmnI GCTCC 6 cut(s) 617, 1097, 1220, 1564, 1667, 1797
Lsp1109I GCAGC 9 cut(s) 89, 277, 811, 892, 1247, 1348, 1642, 1792, 1946
LweI GCATC 4 cut(s) 64, 892, 2018, 2355
MaeI CTAG 9 cut(s) 270, 407, 1101, 1125, 2061, 2133, 2193, 2217, 2374
MaeII ACGT 4 cut(s) 16, 167, 1477, 1521
MaeIII GTNAC 9 cut(s) 431, 693, 701, 914, 1063, 1517, 1522, 2263, 2369
MfeI CAATTG 1 cut(s) 2091
MflI RGATCY 3 cut(s) 331, 803, 1440
MhlI GDGCHC 5 cut(s) 244, 1672, 1802, 1996, 2188
MlsI TGGCCA 1 cut(s) 844
MluNI TGGCCA 1 cut(s) 844
MlyI GAGTC 3 cut(s) 656, 1868, 2359
MmeI TCCRAC 5 cut(s) 1075, 1360, 2055, 2076, 2307
Mox20I TGGCCA 1 cut(s) 844
Mph1103I ATGCAT 1 cut(s) 2011
MroXI GAANNNNTTC 3 cut(s) 145, 1154, 2255
MscI TGGCCA 1 cut(s) 844
MseI TTAA 9 cut(s) 96, 368, 630, 1088, 1299, 1395, 1617, 1812, 2078
MslI CAYNNNNRTG 5 cut(s) 150, 553, 752, 783, 2044
Msp20I TGGCCA 1 cut(s) 844
MspA1I CMGCKG 4 cut(s) 740, 1034, 1326, 1805
MspCI CTTAAG 1 cut(s) 1616
MspI CCGG 4 cut(s) 506, 758, 1638, 1944
MspR9I CCNGG 8 cut(s) 401, 655, 707, 759, 1081, 1638, 1944, 2160
MunI CAATTG 1 cut(s) 2091
MvaI CCWGG 5 cut(s) 401, 655, 707, 1081, 2160
MvnI CGCG 6 cut(s) 700, 819, 1116, 1326, 1845, 1932
NciI CCSGG 3 cut(s) 759, 1638, 1944
NcoI CCATGG 2 cut(s) 151, 715
NdeI CATATG 2 cut(s) 2167, 2176
NheI GCTAGC 1 cut(s) 1100
NlaIV GGNNCC 7 cut(s) 242, 333, 805, 1077, 1222, 1279, 1799
NmuCI GTSAC 7 cut(s) 431, 693, 701, 914, 1063, 1517, 2369
NruI TCGCGA 1 cut(s) 1116
NsiI ATGCAT 1 cut(s) 2011
NspI RCATGY 1 cut(s) 93
NspV TTCGAA 1 cut(s) 2290
PaeI GCATGC 1 cut(s) 93
PdmI GAANNNNTTC 3 cut(s) 145, 1154, 2255
PfeI GAWTC 7 cut(s) 509, 953, 1014, 1256, 1685, 1724, 1871
PflMI CCANNNNNTGG 1 cut(s) 851
PleI GAGTC 3 cut(s) 655, 1867, 2358
PpsI GAGTC 3 cut(s) 655, 1867, 2358
Psp6I CCWGG 5 cut(s) 399, 653, 705, 1079, 2158
PspGI CCWGG 5 cut(s) 399, 653, 705, 1079, 2158
PspN4I GGNNCC 7 cut(s) 242, 333, 805, 1077, 1222, 1279, 1799
PspOMI GGGCCC 1 cut(s) 240
PspPI GGNCC 5 cut(s) 240, 241, 539, 1046, 1589
PsrI GAACNNNNNNTAC 2 cut(s) 2163, 2195
PstI CTGCAG 1 cut(s) 295
PsuI RGATCY 3 cut(s) 331, 803, 1440
PvuII CAGCTG 2 cut(s) 740, 1034
RruI TCGCGA 1 cut(s) 1116
RsaI GTAC 8 cut(s) 134, 203, 426, 626, 874, 1490, 1597, 2155
RsaNI GTAC 8 cut(s) 133, 202, 425, 625, 873, 1489, 1596, 2154
RseI CAYNNNNRTG 5 cut(s) 150, 553, 752, 783, 2044
SacII CCGCGG 1 cut(s) 1327
SaqAI TTAA 9 cut(s) 96, 368, 630, 1088, 1299, 1395, 1617, 1812, 2078
Sau96I GGNCC 5 cut(s) 240, 241, 539, 1046, 1589
SchI GAGTC 3 cut(s) 656, 1868, 2359
ScrFI CCNGG 8 cut(s) 401, 655, 707, 759, 1081, 1638, 1944, 2160
SduI GDGCHC 5 cut(s) 244, 1672, 1802, 1996, 2188
SfaNI GCATC 4 cut(s) 64, 892, 2018, 2355
SfcI CTRYAG 2 cut(s) 291, 957
Sfr303I CCGCGG 1 cut(s) 1327
SfuI TTCGAA 1 cut(s) 2290
SgrBI CCGCGG 1 cut(s) 1327
SinI GGWCC 2 cut(s) 539, 1589
SmiMI CAYNNNNRTG 5 cut(s) 150, 553, 752, 783, 2044
SmlI CTYRAG 1 cut(s) 1616
SmoI CTYRAG 1 cut(s) 1616
SpeI ACTAGT 1 cut(s) 1124
SphI GCATGC 1 cut(s) 93
SsiI CCGC 8 cut(s) 185, 698, 894, 1001, 1324, 1326, 1446, 1803
SspI AATATT 1 cut(s) 2025
SspMI CTAG 9 cut(s) 270, 407, 1101, 1125, 2061, 2133, 2193, 2217, 2374
StyD4I CCNGG 8 cut(s) 399, 653, 705, 757, 1079, 1636, 1942, 2158
StyI CCWWGG 4 cut(s) 151, 715, 1054, 1878
TaaI ACNGT 5 cut(s) 220, 920, 1063, 1093, 1540
TaiI ACGT 4 cut(s) 19, 170, 1480, 1524
TaqI TCGA 4 cut(s) 174, 180, 440, 2290
TaqII GACCGA 2 cut(s) 1606, 1757
TatI WGTACW 3 cut(s) 132, 201, 872
TauI GCSGC 3 cut(s) 188, 896, 1004
TfiI GAWTC 7 cut(s) 509, 953, 1014, 1256, 1685, 1724, 1871
Tru1I TTAA 9 cut(s) 96, 368, 630, 1088, 1299, 1395, 1617, 1812, 2078
Tru9I TTAA 9 cut(s) 96, 368, 630, 1088, 1299, 1395, 1617, 1812, 2078
TscAI CASTG 4 cut(s) 499, 796, 919, 1068
TseFI GTSAC 7 cut(s) 431, 693, 701, 914, 1063, 1517, 2369
TseI GCWGC 9 cut(s) 77, 290, 824, 905, 1235, 1361, 1655, 1805, 1934
Tsp45I GTSAC 7 cut(s) 431, 693, 701, 914, 1063, 1517, 2369
TspDTI ATGAA 4 cut(s) 122, 378, 543, 1563
TspGWI ACGGA 2 cut(s) 450, 931
TspRI CASTG 4 cut(s) 499, 796, 919, 1068
Van91I CCANNNNNTGG 1 cut(s) 851
Vha464I CTTAAG 1 cut(s) 1616
VneI GTGCAC 1 cut(s) 2184
VpaK11BI GGWCC 2 cut(s) 539, 1589
XagI CCTNNNNNAGG 1 cut(s) 1053
XapI RAATTY 7 cut(s) 109, 193, 684, 1495, 1981, 2228, 2255
XbaI TCTAGA 1 cut(s) 2132
XceI RCATGY 1 cut(s) 93
XcmI CCANNNNNNNNNTGG 1 cut(s) 713
XmnI GAANNNNTTC 3 cut(s) 145, 1154, 2255
XspI CTAG 9 cut(s) 270, 407, 1101, 1125, 2061, 2133, 2193, 2217, 2374
Zsp2I ATGCAT 1 cut(s) 2011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.