pycom11g03350

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000923
Physical Location & Seq
Forward (+)
2617568 .. 2619193
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g03350.3

Sequence Viewer

Length: 597 bp
ATGCAGAAGGAGGAAGCAATTTTTGCTTCCAAAAACAGGCAAAAACGGAGTTTTAAAAACAGGAGAAGGAAGAGCCTCTTTCTACGCACACTTCTTCTCTTTACAAAATATTGGCAGATGGGAAATTCAATTCTCCCTTCTTTTCTTCTCTCAGTTCTTCTCATCTCCTTGTTTCATATTCCAGCACATGCTGCCAAAAGTTCTTACATTGTGTACTTAGGAGCAGGTCCTCATGTTCTGGACCCTTTGTCCGCCGATCTCGATAGCGTGACAAATTCTCATTACAATCTTCTTGGAACAGTTTTGGGAAGCAATGAAAGGGCACAGGAATCAATATTTTACTCATATACGAGAAATATCAATGGTTTTGCTGCAATTTTGGATGATGAGGAGGCTGCTCAGATTGCAAAGGATCCAAATGTTGTGTCTGTTTTCCCAAACAGAGGAAGAACACTACACACAACTCGTTCATGGGATTTTCTTCAATTGGAAGAAAACGACGAAATCCGGGCTGGCTCTATTTGGAAGAAGGCACGGTTAGGTGCAAATACAATTATCGGAAACCTTGATACTGTTGATATATTTCCTGTGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

199

Amino Acids

22.31

Weight (kDa)

9.36

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 215
AciI CCGC 1 cut(s) 252
AclWI GGATC 2 cut(s) 407, 420
AcsI RAATTY 2 cut(s) 124, 274
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 2 cut(s) 36, 443
AgsI TTSAA 2 cut(s) 129, 485
AhdI GACNNNNNGTC 1 cut(s) 247
AlwI GGATC 2 cut(s) 407, 420
ApeKI GCWGC 3 cut(s) 191, 371, 395
ApoI RAATTY 2 cut(s) 124, 274
ArsI GACNNNNNNTTYG 2 cut(s) 410, 442
AspS9I GGNCC 2 cut(s) 227, 241
AsuC2I CCSGG 1 cut(s) 509
AvaII GGWCC 2 cut(s) 227, 241
BaeGI GKGCMC 1 cut(s) 325
BamHI GGATCC 1 cut(s) 412
BbvI GCAGC 3 cut(s) 178, 358, 382
BccI CCATC 1 cut(s) 112
BcnI CCSGG 1 cut(s) 509
BfuAI ACCTGC 1 cut(s) 215
BisI GCNGC 3 cut(s) 192, 372, 396
BlsI GCNGC 3 cut(s) 193, 373, 397
Bme1390I CCNGG 1 cut(s) 509
Bme18I GGWCC 2 cut(s) 227, 241
BmeRI GACNNNNNGTC 1 cut(s) 247
BmgT120I GGNCC 2 cut(s) 227, 241
BmiI GGNNCC 2 cut(s) 243, 414
BmrFI CCNGG 1 cut(s) 509
BpuMI CCSGG 1 cut(s) 509
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 443
Bse3DI GCAATG 1 cut(s) 319
BseGI GGATG 1 cut(s) 388
BseLI CCNNNNNNNGG 2 cut(s) 36, 443
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 2 cut(s) 165, 413
BseRI GAGGAG 1 cut(s) 404
BseSI GKGCMC 1 cut(s) 325
BseXI GCAGC 3 cut(s) 178, 358, 382
BsiSI CCGG 1 cut(s) 508
BslI CCNNNNNNNGG 2 cut(s) 36, 443
Bsp1286I GDGCHC 1 cut(s) 325
Bsp143I GATC 2 cut(s) 256, 412
BspACI CCGC 1 cut(s) 252
BspCNI CTCAG 2 cut(s) 164, 412
BspLI GGNNCC 2 cut(s) 243, 414
BspMI ACCTGC 1 cut(s) 215
BspPI GGATC 2 cut(s) 407, 420
BspQI GCTCTTC 1 cut(s) 65
BsrDI GCAATG 1 cut(s) 319
BssMI GATC 2 cut(s) 256, 412
Bst4CI ACNGT 3 cut(s) 301, 537, 574
Bst6I CTCTTC 1 cut(s) 65
BstAPI GCANNNNNTGC 2 cut(s) 23, 191
BstC8I GCNNGC 1 cut(s) 514
BstDEI CTNAG 3 cut(s) 151, 217, 399
BstF5I GGATG 1 cut(s) 388
BstKTI GATC 2 cut(s) 259, 415
BstMBI GATC 2 cut(s) 256, 412
BstMWI GCNNNNNNNGC 3 cut(s) 23, 191, 404
BstNSI RCATGY 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 507
BstSLI GKGCMC 1 cut(s) 325
BstV1I GCAGC 3 cut(s) 178, 358, 382
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
BtsCI GGATG 1 cut(s) 388
BveI ACCTGC 1 cut(s) 215
Cac8I GCNNGC 1 cut(s) 514
Cfr13I GGNCC 2 cut(s) 227, 241
Csp6I GTAC 1 cut(s) 214
CviAII CATG 3 cut(s) 188, 233, 471
CviJI RGCY 4 cut(s) 75, 395, 512, 516
CviKI_1 RGCY 4 cut(s) 75, 395, 512, 516
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 3 cut(s) 151, 217, 399
DpnI GATC 2 cut(s) 258, 414
DpnII GATC 2 cut(s) 256, 412
DraI TTTAAA 1 cut(s) 55
DriI GACNNNNNGTC 1 cut(s) 247
Eam1104I CTCTTC 1 cut(s) 65
Eam1105I GACNNNNNGTC 1 cut(s) 247
EarI CTCTTC 1 cut(s) 65
EciI GGCGGA 1 cut(s) 241
Eco47I GGWCC 2 cut(s) 227, 241
EcoO109I RGGNCCY 1 cut(s) 227
FaeI CATG 3 cut(s) 191, 236, 474
FaiI YATR 7 cut(s) 177, 189, 234, 346, 348, 472, 581
FalI AAGNNNNNCTT 2 cut(s) 62, 94
FatI CATG 3 cut(s) 187, 232, 470
Fnu4HI GCNGC 3 cut(s) 192, 372, 396
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 3 cut(s) 192, 372, 396
GluI GCNGC 3 cut(s) 192, 372, 396
HapII CCGG 1 cut(s) 508
Hin1II CATG 3 cut(s) 191, 236, 474
HinfI GANTC 1 cut(s) 329
HpaII CCGG 1 cut(s) 508
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 2 cut(s) 402, 560
Hpy188III TCNNGA 2 cut(s) 239, 260
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 1 cut(s) 503
HpyAV CCTTC 3 cut(s) 60, 147, 523
HpyCH4III ACNGT 3 cut(s) 301, 537, 574
HpyCH4V TGCA 4 cut(s) 4, 374, 407, 545
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 191, 404
HpyF3I CTNAG 3 cut(s) 151, 217, 399
Hsp92II CATG 3 cut(s) 191, 236, 474
Kzo9I GATC 2 cut(s) 256, 412
LguI GCTCTTC 1 cut(s) 65
LmnI GCTCC 1 cut(s) 221
LpnPI CCDG 8 cut(s) 22, 46, 195, 210, 224, 311, 498, 521
Lsp1109I GCAGC 3 cut(s) 178, 358, 382
MaeIII GTNAC 1 cut(s) 268
MalI GATC 2 cut(s) 258, 414
MboI GATC 2 cut(s) 256, 412
MboII GAAGA 9 cut(s) 82, 86, 137, 149, 281, 459, 473, 503, 538
MfeI CAATTG 1 cut(s) 485
MflI RGATCY 1 cut(s) 412
MhlI GDGCHC 1 cut(s) 325
MluCI AATT 7 cut(s) 18, 124, 129, 274, 375, 485, 552
MnlI CCTC 6 cut(s) 4, 86, 240, 382, 385, 437
MseI TTAA 1 cut(s) 54
MspI CCGG 1 cut(s) 508
MspR9I CCNGG 1 cut(s) 509
MunI CAATTG 1 cut(s) 485
MwoI GCNNNNNNNGC 3 cut(s) 23, 191, 404
NciI CCSGG 1 cut(s) 509
NdeII GATC 2 cut(s) 256, 412
NlaIII CATG 3 cut(s) 191, 236, 474
NlaIV GGNNCC 2 cut(s) 243, 414
NmuCI GTSAC 1 cut(s) 268
NspI RCATGY 1 cut(s) 191
PciSI GCTCTTC 1 cut(s) 65
PfeI GAWTC 1 cut(s) 329
PkrI GCNGC 3 cut(s) 193, 373, 397
PpuMI RGGWCCY 1 cut(s) 227
Psp5II RGGWCCY 1 cut(s) 227
PspN4I GGNNCC 2 cut(s) 243, 414
PspPI GGNCC 2 cut(s) 227, 241
PspPPI RGGWCCY 1 cut(s) 227
PsuI RGATCY 1 cut(s) 412
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SapI GCTCTTC 1 cut(s) 65
SaqAI TTAA 1 cut(s) 54
SatI GCNGC 3 cut(s) 192, 372, 396
Sau3AI GATC 2 cut(s) 256, 412
Sau96I GGNCC 2 cut(s) 227, 241
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 1 cut(s) 325
SetI ASST 3 cut(s) 229, 544, 567
SinI GGWCC 2 cut(s) 227, 241
Sse9I AATT 7 cut(s) 18, 124, 129, 274, 375, 485, 552
SsiI CCGC 1 cut(s) 252
SspI AATATT 2 cut(s) 110, 336
StyD4I CCNGG 1 cut(s) 507
TaaI ACNGT 3 cut(s) 301, 537, 574
TaqI TCGA 1 cut(s) 261
TasI AATT 7 cut(s) 18, 124, 129, 274, 375, 485, 552
TatI WGTACW 1 cut(s) 213
TfiI GAWTC 1 cut(s) 329
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 3 cut(s) 191, 371, 395
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 3 cut(s) 164, 330, 459
TspGWI ACGGA 1 cut(s) 61
VpaK11BI GGWCC 2 cut(s) 227, 241
XapI RAATTY 2 cut(s) 124, 274
XceI RCATGY 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.