FvH4_3g42041

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000923
Physical Location & Seq
Reverse (-)
35155908 .. 35158993
3086 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g42041.t1

Sequence Viewer

Length: 2124 bp
ATGTTTTACTCCTATACTGGCCAGATCAATGGCTTTGCTGCTGTCTTAGATGAAGAAGAGGCTTCACAGATTGCTAAGAATCCAAATGTTGTTTCTGTTTTACCAAACAGAAAAGTAGAACTACATACAACTCATTCATGGGAATTTATGGAATTAGAAGACAAAGCTGGAGTACCTCGTCCCGGTTCTCTTTGGAAGAAAGCAAAGTTTGGTGAAAATATTATCATTGGAAACCTTGACACTGGTGTGTGGCCGGAATCAGAGAGCTTTAACGACGAAGGATTTGGACCAATCCCATCCAAGTGGCGTGGAGCTTGTCTGTTTGACACCAAAGACAAAGTTCGTTGCAACAGGAAGCTAATCGGAGCAAGGTACTTTAACAAAGGCTACTTGGCGAATGTCAAACTCAATAAGGACACCATTCCACCCAGTCTTCTCGTCTCCCCTCGTGACCAGGAAGGCCATGGCTCACATACCCTTTCGACAGCCGGTGGCAGTTTTGTGCCGGGGGCTAATGCTTTTGGAAATGGAAATGGCACTGCTAAAGGTGGATCCCCGAAAGCTCGTGTTGCTGCTTACAAAGTGTGTTGGCCTAGTGTTGATGGCTATACAGAAGGAGGGTGTTTCGCTGCAGACATCTTTGCCGCGTTTGATGCTGCAATCAGTGACGGTGTTGATGTTCTTTCTTTGTCTCTTGGTGGTAATGCTGTAGAGTTTTCGCAGGATGAGATCTCAATAGGAGCGTTCCATGCCGATAAGCATGGAATCGTTGTGGTGTGCTCAGCTGGTAATTCAGGGCCTACTCCGGGTACAGTGACTAATGTTGCACCTTGGATGTTAACAGTTGGAGCTAGCACCCTTGATCGCGAATTCACTAGTTTTCTAACCTTTGGAAGGGGGAAGCATCTGAAGGGAACAAGTCTTTCCTCAACAGGTTTGCCATCTAAAAAGTATTTTCCTTTGATAAGTGGAGCCAATGCTAATGCAGCTACTGCGCGTCCTGAAGATTCCTTGCTATGTTTGAATGGAACCCTCAACCCAAAGAAGGTGAAGGGAAAAATCTTGGTATGTCTACGTGGTGGTGATATTTCGAGGACAGAAAAGGGCGTGATGGCAGCCCTTGCTGGTGCTGTTGGAATGGTTTTGGTTAATGATGAGGAAAGTGGAAATGACATTACAGCTGATCCTCATGTGCTCCCGGCTACACATCTCACTTACACTGATGGAAAACGTGTCTTTGAGTACTTGAATTCTACCAAGACTCCTTTGGCTCGCATTACTCGTGTTAAGACGGTACATGGAACAAAGCCAGCTCCAGTTATGGCTGCTTTCTCATCTCGGGGACCAAGTCCCATTGAGCCATCAATCCTCAAGCCTGATATCACAGCACCAGGAGTGGATATAATTGCTGCTTTTACTGGAGCAACTAGTCCAACTGACCCGTCTAATAAGCCTCGCCCACGTTACATTACAGAATCTGGAACTTCTATGTCATGCCCTCATGTATCTGGAATCGTTGGTCTTCTGAAAACACTCTACCCGACATGGAGCCCTGCTGCTATTAGATCTGCAATCATAACCACAGCAAGAAGATGGGACAATGTAGGGGAGTCAATTTTGGATTCAACCAAGGAACCGGCAACACCATTTGCTTATGGTGCAGGCCATGTTAATCCGAATCGCGCAGCAGATCCGGGACTTGTCTATGACTTAACTACCAATGATTACTTGAATTTCTTATGTGCTCGTGGATACAATGCATCAGTTCTCAAACTATTTTCCGACAAGCCAGTCAAATGTTCCAGGACCTCACACGTTGCTGATCTGAACTATCCTACAATAGCAGTTCCTGATCTTCAGGACAAACCAATTACCATTTATAGAAGAGTGAAGAATGTTGGTACTCCAGGCACATATGTAGCACAGGTTCAAGCACCAGCTGGAGTTTCTGTTTCGGTTCAGCCTAGCAAGCTGGAATTTAAGTCTGTTGGTGAAGAGAAGGAATTCAAGGTTACGCTGAAGGCGAATAGTAAGGTTCGAAATCAAGACTATGTGTTTGGAGAATTGAAATGGTCTGATGGCAAGCACTATGTGACGAGTCCTATTGCTGTGATGCAATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

708

Amino Acids

75.54

Weight (kDa)

8.81

Isoelectric Point (pI)

29.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 2 - 42 1e-10 Peptidase inhibitor I9
Peptidase_S8 PF00082 71 - 555 3.4e-40 Subtilase family
PA PF02225 337 - 411 3.5e-14 PA domain
fn3_6 PF17766 608 - 704 4.2e-30 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1790
AccI GTMKAC 1 cut(s) 1072
AccII CGCG 4 cut(s) 647, 867, 997, 1683
AciI CCGC 1 cut(s) 645
AclWI GGATC 4 cut(s) 546, 559, 1178, 1685
AcoI YGGCCR 2 cut(s) 19, 251
AcsI RAATTY 6 cut(s) 143, 869, 1249, 1732, 1976, 2003
AcuI CTGAAG 4 cut(s) 929, 1023, 1841, 2039
AdeI CACNNNGTG 1 cut(s) 2092
AfaI GTAC 6 cut(s) 174, 374, 811, 1244, 1296, 1903
AfiI CCNNNNNNNGG 4 cut(s) 182, 806, 894, 1045
AflIII ACRYGT 2 cut(s) 1231, 1813
AgsI TTSAA 7 cut(s) 1024, 1249, 1626, 1732, 1931, 2008, 2068
AhlI ACTAGT 2 cut(s) 875, 1427
AjnI CCWGG 4 cut(s) 453, 1390, 1802, 1906
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
AleI CACNNNNGTG 1 cut(s) 245
Alw21I GWGCWC 3 cut(s) 782, 1197, 1747
Alw26I GTCTC 2 cut(s) 445, 696
AlwI GGATC 4 cut(s) 546, 559, 1178, 1685
AlwNI CAGNNNCTG 3 cut(s) 992, 1478, 1850
Ama87I CYCGRG 1 cut(s) 1338
AoxI GGCC 6 cut(s) 19, 251, 460, 590, 797, 1663
ApoI RAATTY 6 cut(s) 143, 869, 1249, 1732, 1976, 2003
ArsI GACNNNNNNTTYG 4 cut(s) 266, 298, 2039, 2071
Asp700I GAANNNNTTC 1 cut(s) 2003
AspLEI GCGC 2 cut(s) 997, 1685
AspS9I GGNCC 4 cut(s) 287, 797, 1343, 1806
AsuC2I CCSGG 5 cut(s) 183, 507, 807, 1199, 1695
AsuHPI GGTGA 4 cut(s) 224, 1060, 1094, 2003
AsuII TTCGAA 1 cut(s) 2038
AsuNHI GCTAGC 1 cut(s) 851
AvaI CYCGRG 1 cut(s) 1338
AvaII GGWCC 3 cut(s) 287, 1343, 1806
BalI TGGCCA 1 cut(s) 21
BamHI GGATCC 1 cut(s) 551
BanII GRGCYC 1 cut(s) 1553
BauI CACGAG 4 cut(s) 447, 564, 1281, 1746
BbsI GAAGAC 3 cut(s) 165, 425, 1514
Bbv12I GWGCWC 3 cut(s) 782, 1197, 1747
BccI CCATC 8 cut(s) 304, 596, 949, 1105, 1217, 1369, 1587, 2072
BciT130I CCWGG 4 cut(s) 455, 1392, 1804, 1908
BciVI GTATCC 1 cut(s) 1745
BcnI CCSGG 5 cut(s) 183, 507, 807, 1199, 1695
BcoDI GTCTC 2 cut(s) 445, 696
BcuI ACTAGT 2 cut(s) 875, 1427
BfaI CTAG 6 cut(s) 594, 852, 876, 1428, 1965, 2122
BfmI CTRYAG 2 cut(s) 630, 708
BfuI GTATCC 1 cut(s) 1745
BglII AGATCT 2 cut(s) 729, 1565
BlpI GCTNAGC 1 cut(s) 781
BmcAI AGTACT 1 cut(s) 1244
Bme1390I CCNGG 9 cut(s) 183, 455, 507, 807, 1199, 1392, 1695, 1804, 1908
Bme18I GGWCC 3 cut(s) 287, 1343, 1806
BmeT110I CYCGRG 1 cut(s) 1338
BmgT120I GGNCC 4 cut(s) 287, 797, 1343, 1806
BmiI GGNNCC 6 cut(s) 553, 973, 1030, 1344, 1550, 1635
BmrFI CCNGG 9 cut(s) 183, 455, 507, 807, 1199, 1392, 1695, 1804, 1908
BmrI ACTGGG 1 cut(s) 423
BmsI GCATC 4 cut(s) 643, 913, 1769, 2103
BmtI GCTAGC 1 cut(s) 855
BmuI ACTGGG 1 cut(s) 423
BpiI GAAGAC 3 cut(s) 165, 425, 1514
BpmI CTGGAG 5 cut(s) 189, 1299, 1440, 1890, 1962
Bpu1102I GCTNAGC 1 cut(s) 781
Bpu14I TTCGAA 1 cut(s) 2038
BpuEI CTTGAG 1 cut(s) 1355
BpuMI CCSGG 5 cut(s) 183, 507, 807, 1199, 1695
BsaAI YACGTR 1 cut(s) 1076
BsaJI CCNNGG 4 cut(s) 463, 506, 830, 1629
BsaXI ACNNNNNCTCC 4 cut(s) 1246, 1276, 1935, 1965
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 806, 894, 1045
Bse118I RCCGGY 2 cut(s) 488, 1636
Bse1I ACTGG 6 cut(s) 22, 247, 429, 1316, 1423, 1790
BseBI CCWGG 4 cut(s) 455, 1392, 1804, 1908
BseDI CCNNGG 4 cut(s) 463, 506, 830, 1629
BseGI GGATG 3 cut(s) 296, 730, 840
BseLI CCNNNNNNNGG 4 cut(s) 182, 806, 894, 1045
BseMII CTCAG 1 cut(s) 795
BseNI ACTGG 6 cut(s) 22, 247, 429, 1316, 1423, 1790
BsgI GTGCAG 1 cut(s) 1680
Bsh1236I CGCG 4 cut(s) 647, 867, 997, 1683
BshFI GGCC 6 cut(s) 21, 253, 462, 592, 799, 1665
BsiHKAI GWGCWC 3 cut(s) 782, 1197, 1747
BsiHKCI CYCGRG 1 cut(s) 1338
BsiSI CCGG 8 cut(s) 183, 254, 489, 506, 806, 1199, 1637, 1694
BslFI GGGAC 5 cut(s) 165, 1335, 1356, 1610, 1710
BslI CCNNNNNNNGG 4 cut(s) 182, 806, 894, 1045
BsmAI GTCTC 2 cut(s) 445, 696
BsmBI CGTCTC 1 cut(s) 445
BsmFI GGGAC 5 cut(s) 165, 1335, 1356, 1610, 1710
BsnI GGCC 6 cut(s) 21, 253, 462, 592, 799, 1665
BsoBI CYCGRG 1 cut(s) 1338
Bsp119I TTCGAA 1 cut(s) 2038
Bsp1286I GDGCHC 4 cut(s) 782, 1197, 1553, 1747
Bsp143I GATC 9 cut(s) 24, 551, 729, 862, 1183, 1565, 1690, 1822, 1852
Bsp1720I GCTNAGC 1 cut(s) 781
Bsp19I CCATGG 1 cut(s) 463
Bsp68I TCGCGA 1 cut(s) 867
BspACI CCGC 1 cut(s) 645
BspANI GGCC 6 cut(s) 21, 253, 462, 592, 799, 1665
BspCNI CTCAG 1 cut(s) 794
BspFNI CGCG 4 cut(s) 647, 867, 997, 1683
BspLI GGNNCC 6 cut(s) 553, 973, 1030, 1344, 1550, 1635
BspMAI CTGCAG 1 cut(s) 634
BspOI GCTAGC 1 cut(s) 855
BspPI GGATC 4 cut(s) 546, 559, 1178, 1685
BspT104I TTCGAA 1 cut(s) 2038
BsrFI RCCGGY 2 cut(s) 488, 1636
BsrI ACTGG 6 cut(s) 22, 247, 429, 1316, 1423, 1790
BssAI RCCGGY 2 cut(s) 488, 1636
BssECI CCNNGG 4 cut(s) 463, 506, 830, 1629
BssMI GATC 9 cut(s) 24, 551, 729, 862, 1183, 1565, 1690, 1822, 1852
BssSI CACGAG 4 cut(s) 447, 564, 1281, 1746
BssT1I CCWWGG 3 cut(s) 463, 830, 1629
Bst2BI CACGAG 4 cut(s) 447, 564, 1281, 1746
Bst2UI CCWGG 4 cut(s) 455, 1392, 1804, 1908
Bst4CI ACNGT 4 cut(s) 671, 814, 844, 1294
Bst6I CTCTTC 3 cut(s) 51, 1879, 1989
BstAPI GCANNNNNTGC 2 cut(s) 992, 1121
BstBAI YACGTR 1 cut(s) 1076
BstBI TTCGAA 1 cut(s) 2038
BstC8I GCNNGC 6 cut(s) 853, 1273, 1311, 1663, 1970, 2084
BstDEI CTNAG 3 cut(s) 46, 75, 781
BstDSI CCRYGG 1 cut(s) 463
BstENI CCTNNNNNAGG 1 cut(s) 892
BstF5I GGATG 3 cut(s) 296, 730, 840
BstFNI CGCG 4 cut(s) 647, 867, 997, 1683
BstHHI GCGC 2 cut(s) 997, 1685
BstKTI GATC 9 cut(s) 27, 554, 732, 865, 1186, 1568, 1693, 1825, 1855
BstMAI GTCTC 2 cut(s) 445, 696
BstMBI GATC 9 cut(s) 24, 551, 729, 862, 1183, 1565, 1690, 1822, 1852
BstMWI GCNNNNNNNGC 8 cut(s) 569, 653, 749, 986, 992, 1121, 1658, 1969
BstNI CCWGG 4 cut(s) 455, 1392, 1804, 1908
BstSCI CCNGG 9 cut(s) 181, 453, 505, 805, 1197, 1390, 1693, 1802, 1906
BstSFI CTRYAG 2 cut(s) 630, 708
BstUI CGCG 4 cut(s) 647, 867, 997, 1683
BstV2I GAAGAC 3 cut(s) 165, 425, 1514
BstX2I RGATCY 4 cut(s) 551, 729, 1565, 1690
BstXI CCANNNNNNTGG 2 cut(s) 29, 303
BstYI RGATCY 4 cut(s) 551, 729, 1565, 1690
BsuI GTATCC 1 cut(s) 1745
BsuRI GGCC 6 cut(s) 21, 253, 462, 592, 799, 1665
BtgI CCRYGG 1 cut(s) 463
BtsCI GGATG 3 cut(s) 296, 730, 840
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 5 cut(s) 240, 537, 670, 819, 1218
BtuMI TCGCGA 1 cut(s) 867
Cac8I GCNNGC 6 cut(s) 853, 1273, 1311, 1663, 1970, 2084
CaiI CAGNNNCTG 3 cut(s) 992, 1478, 1850
CfoI GCGC 2 cut(s) 997, 1685
Cfr10I RCCGGY 2 cut(s) 488, 1636
Cfr13I GGNCC 4 cut(s) 287, 797, 1343, 1806
CseI GACGC 1 cut(s) 986
Csp6I GTAC 6 cut(s) 173, 373, 810, 1243, 1295, 1902
CspCI CAANNNNNGTGG 2 cut(s) 289, 324
CviQI GTAC 6 cut(s) 173, 373, 810, 1243, 1295, 1902
DdeI CTNAG 3 cut(s) 46, 75, 781
DpnI GATC 9 cut(s) 26, 553, 731, 864, 1185, 1567, 1692, 1824, 1854
DpnII GATC 9 cut(s) 24, 551, 729, 862, 1183, 1565, 1690, 1822, 1852
DraIII CACNNNGTG 1 cut(s) 2092
DrdI GACNNNNNNGTC 1 cut(s) 1790
DseDI GACNNNNNNGTC 1 cut(s) 1790
EaeI YGGCCR 2 cut(s) 19, 251
Eam1104I CTCTTC 3 cut(s) 51, 1879, 1989
EarI CTCTTC 3 cut(s) 51, 1879, 1989
Eco130I CCWWGG 3 cut(s) 463, 830, 1629
Eco24I GRGCYC 1 cut(s) 1553
Eco32I GATATC 1 cut(s) 1381
Eco47I GGWCC 3 cut(s) 287, 1343, 1806
Eco57I CTGAAG 4 cut(s) 929, 1023, 1841, 2039
Eco88I CYCGRG 1 cut(s) 1338
EcoNI CCTNNNNNAGG 1 cut(s) 892
EcoO109I RGGNCCY 2 cut(s) 797, 1806
EcoRI GAATTC 3 cut(s) 869, 1249, 2003
EcoRII CCWGG 4 cut(s) 453, 1390, 1802, 1906
EcoRV GATATC 1 cut(s) 1381
EcoT14I CCWWGG 3 cut(s) 463, 830, 1629
EcoT22I ATGCAT 1 cut(s) 1762
EcoT38I GRGCYC 1 cut(s) 1553
ErhI CCWWGG 3 cut(s) 463, 830, 1629
Esp3I CGTCTC 1 cut(s) 445
FaqI GGGAC 5 cut(s) 165, 1335, 1356, 1610, 1710
FauNDI CATATG 1 cut(s) 1915
FblI GTMKAC 1 cut(s) 1072
FokI GGATG 3 cut(s) 283, 737, 847
FriOI GRGCYC 1 cut(s) 1553
FspBI CTAG 6 cut(s) 594, 852, 876, 1428, 1965, 2122
GlaI GCGC 2 cut(s) 996, 1684
GsuI CTGGAG 5 cut(s) 189, 1299, 1440, 1890, 1962
HaeIII GGCC 6 cut(s) 21, 253, 462, 592, 799, 1665
HapII CCGG 8 cut(s) 183, 254, 489, 506, 806, 1199, 1637, 1694
HgaI GACGC 1 cut(s) 986
HhaI GCGC 2 cut(s) 997, 1685
Hin6I GCGC 2 cut(s) 995, 1683
HinP1I GCGC 2 cut(s) 995, 1683
HincII GTYRAC 1 cut(s) 840
HindII GTYRAC 1 cut(s) 840
HpaI GTTAAC 1 cut(s) 840
HpaII CCGG 8 cut(s) 183, 254, 489, 506, 806, 1199, 1637, 1694
HphI GGTGA 4 cut(s) 224, 1060, 1094, 2003
Hpy166II GTNNAC 2 cut(s) 840, 1073
Hpy188I TCNGA 8 cut(s) 262, 365, 909, 1527, 1677, 1783, 1827, 2077
Hpy188III TCNNGA 8 cut(s) 449, 866, 1001, 1479, 1509, 1850, 1859, 2045
Hpy8I GTNNAC 2 cut(s) 840, 1073
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 9 cut(s) 272, 452, 608, 888, 904, 1039, 1045, 1993, 2014
HpyCH4III ACNGT 4 cut(s) 671, 814, 844, 1294
HpyCH4IV ACGT 4 cut(s) 1075, 1231, 1462, 1815
HpyCH4V TGCA 9 cut(s) 348, 632, 659, 827, 986, 1571, 1661, 1760, 2116
HpyF10VI GCNNNNNNNGC 8 cut(s) 569, 653, 749, 986, 992, 1121, 1658, 1969
HpyF3I CTNAG 3 cut(s) 46, 75, 781
HpySE526I ACGT 4 cut(s) 1075, 1231, 1462, 1815
HspAI GCGC 2 cut(s) 995, 1683
KspAI GTTAAC 1 cut(s) 840
Kzo9I GATC 9 cut(s) 24, 551, 729, 862, 1183, 1565, 1690, 1822, 1852
LmnI GCTCC 9 cut(s) 311, 365, 740, 848, 971, 1200, 1318, 1421, 1548
LweI GCATC 4 cut(s) 643, 913, 1769, 2103
MaeI CTAG 6 cut(s) 594, 852, 876, 1428, 1965, 2122
MaeII ACGT 4 cut(s) 1075, 1231, 1462, 1815
MaeIII GTNAC 6 cut(s) 449, 665, 814, 1463, 2011, 2092
MalI GATC 9 cut(s) 26, 553, 731, 864, 1185, 1567, 1692, 1824, 1854
MboI GATC 9 cut(s) 24, 551, 729, 862, 1183, 1565, 1690, 1822, 1852
MflI RGATCY 4 cut(s) 551, 729, 1565, 1690
MhlI GDGCHC 4 cut(s) 782, 1197, 1553, 1747
MlsI TGGCCA 1 cut(s) 21
MluNI TGGCCA 1 cut(s) 21
MlyI GAGTC 3 cut(s) 1255, 1619, 2107
MmeI TCCRAC 4 cut(s) 826, 1114, 1457, 1806
Mox20I TGGCCA 1 cut(s) 21
Mph1103I ATGCAT 1 cut(s) 1762
MroXI GAANNNNTTC 1 cut(s) 2003
MscI TGGCCA 1 cut(s) 21
MseI TTAA 8 cut(s) 270, 378, 839, 1149, 1287, 1671, 1712, 1980
MslI CAYNNNNRTG 2 cut(s) 245, 301
Msp20I TGGCCA 1 cut(s) 21
MspA1I CMGCKG 3 cut(s) 785, 1181, 1940
MspI CCGG 8 cut(s) 183, 254, 489, 506, 806, 1199, 1637, 1694
MspR9I CCNGG 9 cut(s) 183, 455, 507, 807, 1199, 1392, 1695, 1804, 1908
MvaI CCWGG 4 cut(s) 455, 1392, 1804, 1908
MvnI CGCG 4 cut(s) 647, 867, 997, 1683
MwoI GCNNNNNNNGC 8 cut(s) 569, 653, 749, 986, 992, 1121, 1658, 1969
NciI CCSGG 5 cut(s) 183, 507, 807, 1199, 1695
NcoI CCATGG 1 cut(s) 463
NdeI CATATG 1 cut(s) 1915
NdeII GATC 9 cut(s) 24, 551, 729, 862, 1183, 1565, 1690, 1822, 1852
NheI GCTAGC 1 cut(s) 851
NlaIV GGNNCC 6 cut(s) 553, 973, 1030, 1344, 1550, 1635
NmuCI GTSAC 4 cut(s) 449, 665, 814, 2092
NruI TCGCGA 1 cut(s) 867
NsiI ATGCAT 1 cut(s) 1762
NspV TTCGAA 1 cut(s) 2038
OliI CACNNNNGTG 1 cut(s) 245
PcsI WCGNNNNNNNCGW 2 cut(s) 1279, 2021
PdmI GAANNNNTTC 1 cut(s) 2003
PfeI GAWTC 8 cut(s) 79, 257, 765, 1007, 1475, 1512, 1622, 1678
PflFI GACNNNGTC 1 cut(s) 1347
PfoI TCCNGGA 2 cut(s) 1693, 1802
PleI GAGTC 3 cut(s) 1255, 1618, 2106
PpsI GAGTC 3 cut(s) 1255, 1618, 2106
Ppu21I YACGTR 1 cut(s) 1076
PpuMI RGGWCCY 1 cut(s) 1806
Psp5II RGGWCCY 1 cut(s) 1806
Psp6I CCWGG 4 cut(s) 453, 1390, 1802, 1906
PspGI CCWGG 4 cut(s) 453, 1390, 1802, 1906
PspN4I GGNNCC 6 cut(s) 553, 973, 1030, 1344, 1550, 1635
PspPI GGNCC 4 cut(s) 287, 797, 1343, 1806
PspPPI RGGWCCY 1 cut(s) 1806
PsrI GAACNNNNNNTAC 2 cut(s) 1911, 1943
PstI CTGCAG 1 cut(s) 634
PstNI CAGNNNCTG 3 cut(s) 992, 1478, 1850
PsuI RGATCY 4 cut(s) 551, 729, 1565, 1690
PsyI GACNNNGTC 1 cut(s) 1347
PvuII CAGCTG 3 cut(s) 785, 1181, 1940
RruI TCGCGA 1 cut(s) 867
RsaI GTAC 6 cut(s) 174, 374, 811, 1244, 1296, 1903
RsaNI GTAC 6 cut(s) 173, 373, 810, 1243, 1295, 1902
RseI CAYNNNNRTG 2 cut(s) 245, 301
SaqAI TTAA 8 cut(s) 270, 378, 839, 1149, 1287, 1671, 1712, 1980
Sau3AI GATC 9 cut(s) 24, 551, 729, 862, 1183, 1565, 1690, 1822, 1852
Sau96I GGNCC 4 cut(s) 287, 797, 1343, 1806
ScaI AGTACT 1 cut(s) 1244
SchI GAGTC 3 cut(s) 1255, 1619, 2107
ScrFI CCNGG 9 cut(s) 183, 455, 507, 807, 1199, 1392, 1695, 1804, 1908
SduI GDGCHC 4 cut(s) 782, 1197, 1553, 1747
SfaNI GCATC 4 cut(s) 643, 913, 1769, 2103
SfcI CTRYAG 2 cut(s) 630, 708
SfuI TTCGAA 1 cut(s) 2038
SinI GGWCC 3 cut(s) 287, 1343, 1806
SmiMI CAYNNNNRTG 2 cut(s) 245, 301
SmlI CTYRAG 1 cut(s) 1370
SmoI CTYRAG 1 cut(s) 1370
SpeI ACTAGT 2 cut(s) 875, 1427
SsiI CCGC 1 cut(s) 645
SspI AATATT 1 cut(s) 220
SspMI CTAG 6 cut(s) 594, 852, 876, 1428, 1965, 2122
StyD4I CCNGG 9 cut(s) 181, 453, 505, 805, 1197, 1390, 1693, 1802, 1906
StyI CCWWGG 3 cut(s) 463, 830, 1629
TaaI ACNGT 4 cut(s) 671, 814, 844, 1294
TaiI ACGT 4 cut(s) 1078, 1234, 1465, 1818
TaqI TCGA 3 cut(s) 482, 1091, 2038
TatI WGTACW 1 cut(s) 1242
TauI GCSGC 1 cut(s) 647
TfiI GAWTC 8 cut(s) 79, 257, 765, 1007, 1475, 1512, 1622, 1678
Tru1I TTAA 8 cut(s) 270, 378, 839, 1149, 1287, 1671, 1712, 1980
Tru9I TTAA 8 cut(s) 270, 378, 839, 1149, 1287, 1671, 1712, 1980
TscAI CASTG 5 cut(s) 247, 544, 670, 819, 1225
TseFI GTSAC 4 cut(s) 449, 665, 814, 2092
Tsp45I GTSAC 4 cut(s) 449, 665, 814, 2092
TspDTI ATGAA 2 cut(s) 66, 126
TspRI CASTG 5 cut(s) 247, 544, 670, 819, 1225
Tth111I GACNNNGTC 1 cut(s) 1347
VpaK11BI GGWCC 3 cut(s) 287, 1343, 1806
XagI CCTNNNNNAGG 1 cut(s) 892
XapI RAATTY 6 cut(s) 143, 869, 1249, 1732, 1976, 2003
XcmI CCANNNNNNNNNTGG 2 cut(s) 461, 1264
XmiI GTMKAC 1 cut(s) 1072
XmnI GAANNNNTTC 1 cut(s) 2003
XspI CTAG 6 cut(s) 594, 852, 876, 1428, 1965, 2122
ZrmI AGTACT 1 cut(s) 1244
Zsp2I ATGCAT 1 cut(s) 1762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.