pycom11g03360

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000923
Physical Location & Seq
Forward (+)
2626220 .. 2627400
1181 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g03360.2

Sequence Viewer

Length: 798 bp
ATGGTTAACTCCACTGCAGCCAAGTCCATCCAGCCCAACGCTCGTGACCATTCCGGTCATGGCTCACACACCCTTTCAACGGCAGGTGGTAATTTTGTTCCTAGAGTAAGTGTTTTCGGTAATGGCAATGGCACTGCAAAGGGTGGATCCCCAAAAGCTCGCGTGGCTGCGTATAAAGTATGCTGGCCTCCAATCAATGGCAACGAGTGCTTCGATGCAGACATCTTGGCCGCATTTGAAGCTGCAATTAATGACGGTGTTGATGTGCTCTCGGTGTCTCTTGGTGGTGCAGCTGCGGAGTTTTTAAGTGATGGGATTGCAATAGGGTCCTTCCATGCTGTTCAGAAGGGGATTACCGTGGTGAGCTCAGCTGGGAATTCAGGTCCTACTCCTGGGTCAGTGTCGAATGTGTCACCGTGGATGCTCACAGTTGGAGCTAGCACAATTGATCGCGAGTTCACCAGTTATGTTACTCTTGGCAACAAGAAGCATCTCAAGGGAGCAAGTCTTTCCTCTGCAGCTTTGCCAGCTAAAAAGTTCTACCCATTGATAAGTGCATGCGATGCTAAAGCTGCTAATGCGTCTGGTTCTGAAGCCCATCTCTGCAAACCTGGAACCCTCGATAAAAAGAAGGCAAGGGGGAAAATTTTGGTTTGCGTTCGAGGGCAAAATCCAAGAGCCGACAAGGGTCAACAAGCTATTCTTGCAGGCGCTGTTGGTATGATTTTGGTTAATGATAAGCTAAGCGGGAATGAAATCATAGCTGATCCATCACTTGCTTCCGGCTTCACACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

266

Amino Acids

26.92

Weight (kDa)

9.32

Isoelectric Point (pI)

17.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 74
Acc36I ACCTGC 1 cut(s) 74
AccB7I CCANNNNNTGG 1 cut(s) 197
AccII CGCG 2 cut(s) 162, 453
AciI CCGC 3 cut(s) 231, 296, 747
AclWI GGATC 3 cut(s) 141, 154, 761
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 2 cut(s) 376, 645
AcuI CTGAAG 1 cut(s) 612
AfiI CCNNNNNNNGG 3 cut(s) 79, 197, 392
AflIII ACRYGT 1 cut(s) 791
AgsI TTSAA 2 cut(s) 78, 239
AjnI CCWGG 2 cut(s) 391, 610
Alw21I GWGCWC 2 cut(s) 270, 368
Alw26I GTCTC 1 cut(s) 282
AlwI GGATC 3 cut(s) 141, 154, 761
AlwNI CAGNNNCTG 1 cut(s) 713
AoxI GGCC 2 cut(s) 185, 228
ApeKI GCWGC 7 cut(s) 17, 167, 242, 290, 293, 518, 572
ApoI RAATTY 2 cut(s) 376, 645
AseI ATTAAT 1 cut(s) 249
AspLEI GCGC 1 cut(s) 713
AspS9I GGNCC 2 cut(s) 327, 383
AsuHPI GGTGA 3 cut(s) 373, 405, 451
AsuNHI GCTAGC 1 cut(s) 437
AvaII GGWCC 2 cut(s) 327, 383
BamHI GGATCC 1 cut(s) 146
BanII GRGCYC 1 cut(s) 368
BauI CACGAG 1 cut(s) 42
Bbv12I GWGCWC 2 cut(s) 270, 368
BbvI GCAGC 7 cut(s) 29, 154, 229, 280, 302, 530, 559
BccI CCATC 4 cut(s) 35, 305, 606, 778
BceAI ACGGC 1 cut(s) 96
BciT130I CCWGG 2 cut(s) 393, 612
BcoDI GTCTC 1 cut(s) 282
BfaI CTAG 2 cut(s) 102, 438
BfmI CTRYAG 2 cut(s) 15, 516
BfoI RGCGCY 1 cut(s) 714
BfuAI ACCTGC 1 cut(s) 74
BisI GCNGC 8 cut(s) 18, 168, 231, 243, 291, 294, 519, 573
BlpI GCTNAGC 2 cut(s) 367, 743
BlsI GCNGC 8 cut(s) 19, 169, 232, 244, 292, 295, 520, 574
Bme1390I CCNGG 2 cut(s) 393, 612
Bme18I GGWCC 2 cut(s) 327, 383
BmgT120I GGNCC 2 cut(s) 327, 383
BmiI GGNNCC 3 cut(s) 148, 328, 616
BmrFI CCNGG 2 cut(s) 393, 612
BmsI GCATC 4 cut(s) 205, 411, 499, 553
BmtI GCTAGC 1 cut(s) 441
BoxI GACNNNNGTC 1 cut(s) 687
Bpu1102I GCTNAGC 2 cut(s) 367, 743
BpuEI CTTGAG 1 cut(s) 479
BsaJI CCNNGG 3 cut(s) 357, 392, 416
BsaWI WCCGGW 1 cut(s) 53
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 197, 392
Bse1I ACTGG 1 cut(s) 462
Bse3DI GCAATG 1 cut(s) 133
BseBI CCWGG 2 cut(s) 393, 612
BseDI CCNNGG 3 cut(s) 357, 392, 416
BseGI GGATG 2 cut(s) 27, 426
BseLI CCNNNNNNNGG 3 cut(s) 79, 197, 392
BseMI GCAATG 1 cut(s) 133
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 1 cut(s) 462
BseXI GCAGC 7 cut(s) 29, 154, 229, 280, 302, 530, 559
BseYI CCCAGC 1 cut(s) 371
BsgI GTGCAG 1 cut(s) 309
Bsh1236I CGCG 2 cut(s) 162, 453
BshFI GGCC 2 cut(s) 187, 230
BsiHKAI GWGCWC 2 cut(s) 270, 368
BsiSI CCGG 2 cut(s) 54, 783
BslI CCNNNNNNNGG 3 cut(s) 79, 197, 392
BsmAI GTCTC 1 cut(s) 282
BsnI GGCC 2 cut(s) 187, 230
Bsp1286I GDGCHC 2 cut(s) 270, 368
Bsp143I GATC 3 cut(s) 146, 448, 766
Bsp1720I GCTNAGC 2 cut(s) 367, 743
Bsp68I TCGCGA 1 cut(s) 453
BspACI CCGC 3 cut(s) 231, 296, 747
BspANI GGCC 2 cut(s) 187, 230
BspCNI CTCAG 1 cut(s) 380
BspFNI CGCG 2 cut(s) 162, 453
BspLI GGNNCC 3 cut(s) 148, 328, 616
BspMAI CTGCAG 2 cut(s) 19, 520
BspMI ACCTGC 1 cut(s) 74
BspOI GCTAGC 1 cut(s) 441
BspPI GGATC 3 cut(s) 141, 154, 761
BsrDI GCAATG 1 cut(s) 133
BsrI ACTGG 1 cut(s) 462
BssECI CCNNGG 3 cut(s) 357, 392, 416
BssMI GATC 3 cut(s) 146, 448, 766
BssSI CACGAG 1 cut(s) 42
Bst2BI CACGAG 1 cut(s) 42
Bst2UI CCWGG 2 cut(s) 393, 612
Bst4CI ACNGT 4 cut(s) 257, 358, 417, 430
BstAPI GCANNNNNTGC 2 cut(s) 207, 563
BstC8I GCNNGC 6 cut(s) 160, 185, 439, 528, 559, 709
BstDEI CTNAG 2 cut(s) 367, 743
BstDSI CCRYGG 2 cut(s) 357, 416
BstF5I GGATG 2 cut(s) 27, 426
BstFNI CGCG 2 cut(s) 162, 453
BstH2I RGCGCY 1 cut(s) 714
BstHHI GCGC 1 cut(s) 713
BstKTI GATC 3 cut(s) 149, 451, 769
BstMAI GTCTC 1 cut(s) 282
BstMBI GATC 3 cut(s) 146, 448, 766
BstMWI GCNNNNNNNGC 8 cut(s) 164, 207, 239, 527, 563, 572, 578, 704
BstNI CCWGG 2 cut(s) 393, 612
BstNSI RCATGY 1 cut(s) 561
BstPAI GACNNNNGTC 1 cut(s) 687
BstSCI CCNGG 2 cut(s) 391, 610
BstSFI CTRYAG 2 cut(s) 15, 516
BstUI CGCG 2 cut(s) 162, 453
BstV1I GCAGC 7 cut(s) 29, 154, 229, 280, 302, 530, 559
BstX2I RGATCY 1 cut(s) 146
BstYI RGATCY 1 cut(s) 146
BsuRI GGCC 2 cut(s) 187, 230
BtgI CCRYGG 2 cut(s) 357, 416
BtgZI GCGATG 1 cut(s) 576
BtsCI GGATG 2 cut(s) 27, 426
BtsI GCAGTG 2 cut(s) 12, 132
BtsIMutI CAGTG 3 cut(s) 12, 132, 405
BtuMI TCGCGA 1 cut(s) 453
BveI ACCTGC 1 cut(s) 74
Cac8I GCNNGC 6 cut(s) 160, 185, 439, 528, 559, 709
CaiI CAGNNNCTG 1 cut(s) 713
CfoI GCGC 1 cut(s) 713
Cfr13I GGNCC 2 cut(s) 327, 383
CseI GACGC 1 cut(s) 570
CviAII CATG 3 cut(s) 59, 335, 558
DdeI CTNAG 2 cut(s) 367, 743
DpnI GATC 3 cut(s) 148, 450, 768
DpnII GATC 3 cut(s) 146, 448, 766
EaeI YGGCCR 1 cut(s) 228
Ecl136II GAGCTC 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 368
Eco47I GGWCC 2 cut(s) 327, 383
Eco53kI GAGCTC 1 cut(s) 366
Eco57I CTGAAG 1 cut(s) 612
EcoICRI GAGCTC 1 cut(s) 366
EcoO109I RGGNCCY 2 cut(s) 327, 383
EcoRI GAATTC 1 cut(s) 376
EcoRII CCWGG 2 cut(s) 391, 610
EcoT38I GRGCYC 1 cut(s) 368
FaeI CATG 3 cut(s) 62, 338, 561
FaiI YATR 8 cut(s) 60, 174, 181, 336, 468, 559, 722, 761
FalI AAGNNNNNCTT 2 cut(s) 687, 719
FatI CATG 3 cut(s) 58, 334, 557
FauI CCCGC 1 cut(s) 740
Fnu4HI GCNGC 8 cut(s) 18, 168, 231, 243, 291, 294, 519, 573
FokI GGATG 2 cut(s) 14, 433
FriOI GRGCYC 1 cut(s) 368
Fsp4HI GCNGC 8 cut(s) 18, 168, 231, 243, 291, 294, 519, 573
FspBI CTAG 2 cut(s) 102, 438
GlaI GCGC 1 cut(s) 712
GluI GCNGC 8 cut(s) 18, 168, 231, 243, 291, 294, 519, 573
GsaI CCCAGC 1 cut(s) 375
HaeII RGCGCY 1 cut(s) 714
HaeIII GGCC 2 cut(s) 187, 230
HapII CCGG 2 cut(s) 54, 783
HgaI GACGC 1 cut(s) 570
HhaI GCGC 1 cut(s) 713
Hin1II CATG 3 cut(s) 62, 338, 561
Hin6I GCGC 1 cut(s) 711
HinP1I GCGC 1 cut(s) 711
HincII GTYRAC 2 cut(s) 7, 692
HindII GTYRAC 2 cut(s) 7, 692
HpaI GTTAAC 1 cut(s) 7
HpaII CCGG 2 cut(s) 54, 783
HphI GGTGA 3 cut(s) 373, 405, 451
Hpy166II GTNNAC 3 cut(s) 7, 459, 692
Hpy188I TCNGA 2 cut(s) 345, 592
Hpy188III TCNNGA 2 cut(s) 44, 452
Hpy8I GTNNAC 3 cut(s) 7, 459, 692
HpyAV CCTTC 3 cut(s) 340, 340, 625
HpyCH4III ACNGT 4 cut(s) 257, 358, 417, 430
HpyCH4IV ACGT 1 cut(s) 793
HpyF10VI GCNNNNNNNGC 8 cut(s) 164, 207, 239, 527, 563, 572, 578, 704
HpyF3I CTNAG 2 cut(s) 367, 743
HpySE526I ACGT 1 cut(s) 793
Hsp92II CATG 3 cut(s) 62, 338, 561
HspAI GCGC 1 cut(s) 711
KspAI GTTAAC 1 cut(s) 7
Kzo9I GATC 3 cut(s) 146, 448, 766
LmnI GCTCC 2 cut(s) 434, 500
Lsp1109I GCAGC 7 cut(s) 29, 154, 229, 280, 302, 530, 559
LweI GCATC 4 cut(s) 205, 411, 499, 553
MaeI CTAG 2 cut(s) 102, 438
MaeII ACGT 1 cut(s) 793
MaeIII GTNAC 3 cut(s) 44, 411, 469
MalI GATC 3 cut(s) 148, 450, 768
MboI GATC 3 cut(s) 146, 448, 766
MfeI CAATTG 1 cut(s) 444
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 2 cut(s) 270, 368
MluCI AATT 5 cut(s) 91, 246, 376, 444, 645
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 4 cut(s) 198, 523, 629, 656
MseI TTAA 5 cut(s) 6, 249, 305, 732, 796
MspA1I CMGCKG 2 cut(s) 293, 371
MspI CCGG 2 cut(s) 54, 783
MspR9I CCNGG 2 cut(s) 393, 612
MunI CAATTG 1 cut(s) 444
MvaI CCWGG 2 cut(s) 393, 612
MvnI CGCG 2 cut(s) 162, 453
MwoI GCNNNNNNNGC 8 cut(s) 164, 207, 239, 527, 563, 572, 578, 704
NdeII GATC 3 cut(s) 146, 448, 766
NheI GCTAGC 1 cut(s) 437
NlaIII CATG 3 cut(s) 62, 338, 561
NlaIV GGNNCC 3 cut(s) 148, 328, 616
NmuCI GTSAC 2 cut(s) 44, 411
NruI TCGCGA 1 cut(s) 453
NspI RCATGY 1 cut(s) 561
PaeI GCATGC 1 cut(s) 561
PaqCI CACCTGC 1 cut(s) 74
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PflMI CCANNNNNTGG 1 cut(s) 197
PkrI GCNGC 8 cut(s) 19, 169, 232, 244, 292, 295, 520, 574
PpuMI RGGWCCY 2 cut(s) 327, 383
PshAI GACNNNNGTC 1 cut(s) 687
PshBI ATTAAT 1 cut(s) 249
Psp124BI GAGCTC 1 cut(s) 368
Psp5II RGGWCCY 2 cut(s) 327, 383
Psp6I CCWGG 2 cut(s) 391, 610
PspFI CCCAGC 1 cut(s) 371
PspGI CCWGG 2 cut(s) 391, 610
PspN4I GGNNCC 3 cut(s) 148, 328, 616
PspPI GGNCC 2 cut(s) 327, 383
PspPPI RGGWCCY 2 cut(s) 327, 383
PstI CTGCAG 2 cut(s) 19, 520
PstNI CAGNNNCTG 1 cut(s) 713
PsuI RGATCY 1 cut(s) 146
PvuII CAGCTG 2 cut(s) 293, 371
RruI TCGCGA 1 cut(s) 453
SacI GAGCTC 1 cut(s) 368
SaqAI TTAA 5 cut(s) 6, 249, 305, 732, 796
SatI GCNGC 8 cut(s) 18, 168, 231, 243, 291, 294, 519, 573
Sau3AI GATC 3 cut(s) 146, 448, 766
Sau96I GGNCC 2 cut(s) 327, 383
ScrFI CCNGG 2 cut(s) 393, 612
SduI GDGCHC 2 cut(s) 270, 368
SfaNI GCATC 4 cut(s) 205, 411, 499, 553
SfcI CTRYAG 2 cut(s) 15, 516
SinI GGWCC 2 cut(s) 327, 383
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
SphI GCATGC 1 cut(s) 561
Sse9I AATT 5 cut(s) 91, 246, 376, 444, 645
SsiI CCGC 3 cut(s) 231, 296, 747
SspMI CTAG 2 cut(s) 102, 438
SstI GAGCTC 1 cut(s) 368
StyD4I CCNGG 2 cut(s) 391, 610
TaaI ACNGT 4 cut(s) 257, 358, 417, 430
TaiI ACGT 1 cut(s) 796
TaqI TCGA 4 cut(s) 213, 404, 621, 661
TasI AATT 5 cut(s) 91, 246, 376, 444, 645
TauI GCSGC 1 cut(s) 233
Tru1I TTAA 5 cut(s) 6, 249, 305, 732, 796
Tru9I TTAA 5 cut(s) 6, 249, 305, 732, 796
TscAI CASTG 3 cut(s) 19, 139, 405
TseFI GTSAC 2 cut(s) 44, 411
TseI GCWGC 7 cut(s) 17, 167, 242, 290, 293, 518, 572
Tsp45I GTSAC 2 cut(s) 44, 411
TspDTI ATGAA 1 cut(s) 768
TspRI CASTG 3 cut(s) 19, 139, 405
Van91I CCANNNNNTGG 1 cut(s) 197
VpaK11BI GGWCC 2 cut(s) 327, 383
VspI ATTAAT 1 cut(s) 249
XapI RAATTY 2 cut(s) 376, 645
XceI RCATGY 1 cut(s) 561
XcmI CCANNNNNNNNNTGG 1 cut(s) 56
XspI CTAG 2 cut(s) 102, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.