subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1316 |
| Acc36I | ACCTGC | 1 cut(s) | 1316 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 815 |
| AccI | GTMKAC | 1 cut(s) | 1454 |
| AccII | CGCG | 1 cut(s) | 663 |
| AciI | CCGC | 2 cut(s) | 806, 912 |
| AclWI | GGATC | 4 cut(s) | 187, 772, 941, 1379 |
| AcoI | YGGCCR | 3 cut(s) | 464, 673, 803 |
| AcsI | RAATTY | 4 cut(s) | 82, 1263, 1936, 2207 |
| AcuI | CTGAAG | 2 cut(s) | 466, 2243 |
| AfaI | GTAC | 4 cut(s) | 98, 587, 791, 2127 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 133, 326, 815, 1010, 1891 |
| AflII | CTTAAG | 1 cut(s) | 1571 |
| AflIII | ACRYGT | 3 cut(s) | 1408, 1870, 2000 |
| AgsI | TTSAA | 8 cut(s) | 12, 1672, 1877, 1936, 2043, 2185, 2212, 2269 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 130 |
| AjnI | CCWGG | 6 cut(s) | 120, 1236, 1591, 1897, 2110, 2167 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 292, 324 |
| Alw21I | GWGCWC | 2 cut(s) | 109, 2245 |
| Alw26I | GTCTC | 1 cut(s) | 900 |
| AlwI | GGATC | 4 cut(s) | 187, 772, 941, 1379 |
| AlwNI | CAGNNNCTG | 5 cut(s) | 704, 1331, 1682, 2054, 2078 |
| Ama87I | CYCGRG | 2 cut(s) | 718, 1010 |
| AoxI | GGCC | 6 cut(s) | 204, 464, 673, 803, 1000, 1631 |
| ApaI | GGGCCC | 1 cut(s) | 208 |
| ApoI | RAATTY | 4 cut(s) | 82, 1263, 1936, 2207 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2240, 2272 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2207 |
| AspLEI | GCGC | 1 cut(s) | 1659 |
| AsuC2I | CCSGG | 2 cut(s) | 1011, 1012 |
| AsuHPI | GGTGA | 5 cut(s) | 759, 917, 1023, 1733, 2207 |
| AsuNHI | GCTAGC | 1 cut(s) | 1055 |
| AvaI | CYCGRG | 2 cut(s) | 718, 1010 |
| AvaII | GGWCC | 6 cut(s) | 124, 295, 500, 945, 1544, 1894 |
| AxyI | CCTNAGG | 1 cut(s) | 2229 |
| BaeGI | GKGCMC | 1 cut(s) | 208 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 444, 477 |
| BalI | TGGCCA | 1 cut(s) | 675 |
| BanII | GRGCYC | 3 cut(s) | 208, 1757, 2303 |
| BauI | CACGAG | 2 cut(s) | 777, 1482 |
| BbsI | GAAGAC | 3 cut(s) | 724, 1496, 1718 |
| Bbv12I | GWGCWC | 2 cut(s) | 109, 2245 |
| BccI | CCATC | 7 cut(s) | 517, 653, 923, 1153, 1418, 1753, 2273 |
| BceAI | ACGGC | 1 cut(s) | 1644 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 329, 363 |
| BciT130I | CCWGG | 6 cut(s) | 122, 1238, 1593, 1899, 2112, 2169 |
| BclI | TGATCA | 1 cut(s) | 1639 |
| BcnI | CCSGG | 2 cut(s) | 1011, 1012 |
| BcoDI | GTCTC | 1 cut(s) | 900 |
| BfaI | CTAG | 4 cut(s) | 1056, 1539, 2085, 2323 |
| BfmI | CTRYAG | 4 cut(s) | 633, 975, 1758, 1785 |
| BfoI | RGCGCY | 1 cut(s) | 1660 |
| BfrI | CTTAAG | 1 cut(s) | 1571 |
| BfuAI | ACCTGC | 1 cut(s) | 1316 |
| BlpI | GCTNAGC | 1 cut(s) | 985 |
| Bme1390I | CCNGG | 8 cut(s) | 122, 1011, 1012, 1238, 1593, 1899, 2112, 2169 |
| Bme18I | GGWCC | 6 cut(s) | 124, 295, 500, 945, 1544, 1894 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 130 |
| BmeT110I | CYCGRG | 2 cut(s) | 718, 1010 |
| BmiI | GGNNCC | 9 cut(s) | 126, 206, 297, 946, 1545, 1754, 1896, 2139, 2300 |
| BmrFI | CCNGG | 8 cut(s) | 122, 1011, 1012, 1238, 1593, 1899, 2112, 2169 |
| BmsI | GCATC | 5 cut(s) | 823, 847, 1117, 1171, 1201 |
| BmtI | GCTAGC | 1 cut(s) | 1059 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1305 |
| BpiI | GAAGAC | 3 cut(s) | 724, 1496, 1718 |
| BpmI | CTGGAG | 4 cut(s) | 1063, 1155, 1641, 2094 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1185 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 985 |
| BpuEI | CTTGAG | 2 cut(s) | 1097, 2229 |
| BpuMI | CCSGG | 2 cut(s) | 1011, 1012 |
| BsaAI | YACGTR | 1 cut(s) | 2122 |
| BsaJI | CCNNGG | 6 cut(s) | 676, 1009, 1010, 1034, 1897, 2007 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2139, 2169 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 133, 326, 815, 1010, 1891 |
| Bse1I | ACTGG | 5 cut(s) | 65, 460, 1080, 1624, 2072 |
| Bse21I | CCTNAGG | 1 cut(s) | 2229 |
| Bse3DI | GCAATG | 6 cut(s) | 202, 556, 745, 751, 826, 1824 |
| BseBI | CCWGG | 6 cut(s) | 122, 1238, 1593, 1899, 2112, 2169 |
| BseDI | CCNNGG | 6 cut(s) | 676, 1009, 1010, 1034, 1897, 2007 |
| BseGI | GGATG | 6 cut(s) | 271, 509, 645, 1216, 1429, 1829 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 133, 326, 815, 1010, 1891 |
| BseMI | GCAATG | 6 cut(s) | 202, 556, 745, 751, 826, 1824 |
| BseMII | CTCAG | 2 cut(s) | 296, 999 |
| BseNI | ACTGG | 5 cut(s) | 65, 460, 1080, 1624, 2072 |
| BseRI | GAGGAG | 1 cut(s) | 619 |
| BseSI | GKGCMC | 1 cut(s) | 208 |
| BseYI | CCCAGC | 1 cut(s) | 1215 |
| BsgI | GTGCAG | 1 cut(s) | 1194 |
| Bsh1236I | CGCG | 1 cut(s) | 663 |
| Bsh1285I | CGRYCG | 1 cut(s) | 138 |
| BshFI | GGCC | 6 cut(s) | 206, 466, 675, 805, 1002, 1633 |
| BsiEI | CGRYCG | 1 cut(s) | 138 |
| BsiHKAI | GWGCWC | 2 cut(s) | 109, 2245 |
| BsiHKCI | CYCGRG | 2 cut(s) | 718, 1010 |
| BsiSI | CCGG | 2 cut(s) | 467, 1011 |
| BslFI | GGGAC | 4 cut(s) | 701, 1027, 1557, 1989 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 133, 326, 815, 1010, 1891 |
| BsmAI | GTCTC | 1 cut(s) | 900 |
| BsmFI | GGGAC | 4 cut(s) | 701, 1027, 1557, 1989 |
| BsmI | GAATGC | 1 cut(s) | 1275 |
| BsnI | GGCC | 6 cut(s) | 206, 466, 675, 805, 1002, 1633 |
| BsoBI | CYCGRG | 2 cut(s) | 718, 1010 |
| Bsp120I | GGGCCC | 1 cut(s) | 204 |
| Bsp1286I | GDGCHC | 5 cut(s) | 109, 208, 1757, 2245, 2303 |
| Bsp143I | GATC | 9 cut(s) | 139, 179, 573, 764, 933, 1066, 1384, 1639, 2056 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 985 |
| Bsp19I | CCATGG | 1 cut(s) | 676 |
| BspACI | CCGC | 2 cut(s) | 806, 912 |
| BspANI | GGCC | 6 cut(s) | 206, 466, 675, 805, 1002, 1633 |
| BspCNI | CTCAG | 2 cut(s) | 295, 998 |
| BspFNI | CGCG | 1 cut(s) | 663 |
| BspHI | TCATGA | 1 cut(s) | 1779 |
| BspLI | GGNNCC | 9 cut(s) | 126, 206, 297, 946, 1545, 1754, 1896, 2139, 2300 |
| BspMAI | CTGCAG | 2 cut(s) | 637, 1762 |
| BspMI | ACCTGC | 1 cut(s) | 1316 |
| BspOI | GCTAGC | 1 cut(s) | 1059 |
| BspPI | GGATC | 4 cut(s) | 187, 772, 941, 1379 |
| BspTI | CTTAAG | 1 cut(s) | 1571 |
| BsrDI | GCAATG | 6 cut(s) | 202, 556, 745, 751, 826, 1824 |
| BsrI | ACTGG | 5 cut(s) | 65, 460, 1080, 1624, 2072 |
| BssECI | CCNNGG | 6 cut(s) | 676, 1009, 1010, 1034, 1897, 2007 |
| BssMI | GATC | 9 cut(s) | 139, 179, 573, 764, 933, 1066, 1384, 1639, 2056 |
| BssSI | CACGAG | 2 cut(s) | 777, 1482 |
| BssT1I | CCWWGG | 2 cut(s) | 676, 2007 |
| Bst2BI | CACGAG | 2 cut(s) | 777, 1482 |
| Bst2UI | CCWGG | 6 cut(s) | 122, 1238, 1593, 1899, 2112, 2169 |
| Bst6I | CTCTTC | 6 cut(s) | 50, 1210, 1540, 1743, 2083, 2193 |
| BstAFI | CTTAAG | 1 cut(s) | 1571 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 825, 1181 |
| BstBAI | YACGTR | 1 cut(s) | 2122 |
| BstC8I | GCNNGC | 5 cut(s) | 661, 991, 1057, 1314, 2177 |
| BstDEI | CTNAG | 8 cut(s) | 100, 282, 639, 985, 1149, 1185, 1361, 2229 |
| BstDSI | CCRYGG | 2 cut(s) | 676, 1034 |
| BstF5I | GGATG | 6 cut(s) | 271, 509, 645, 1216, 1429, 1829 |
| BstFNI | CGCG | 1 cut(s) | 663 |
| BstH2I | RGCGCY | 1 cut(s) | 1660 |
| BstHHI | GCGC | 1 cut(s) | 1659 |
| BstKTI | GATC | 9 cut(s) | 142, 182, 576, 767, 936, 1069, 1387, 1642, 2059 |
| BstMAI | GTCTC | 1 cut(s) | 900 |
| BstMBI | GATC | 9 cut(s) | 139, 179, 573, 764, 933, 1066, 1384, 1639, 2056 |
| BstMCI | CGRYCG | 1 cut(s) | 138 |
| BstNI | CCWGG | 6 cut(s) | 122, 1238, 1593, 1899, 2112, 2169 |
| BstNSI | RCATGY | 3 cut(s) | 1412, 1874, 2004 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1305 |
| BstSCI | CCNGG | 8 cut(s) | 120, 1009, 1010, 1236, 1591, 1897, 2110, 2167 |
| BstSFI | CTRYAG | 4 cut(s) | 633, 975, 1758, 1785 |
| BstSLI | GKGCMC | 1 cut(s) | 208 |
| BstSNI | TACGTA | 1 cut(s) | 2122 |
| BstUI | CGCG | 1 cut(s) | 663 |
| BstV2I | GAAGAC | 3 cut(s) | 724, 1496, 1718 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 516 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2229 |
| BsuRI | GGCC | 6 cut(s) | 206, 466, 675, 805, 1002, 1633 |
| BtgI | CCRYGG | 2 cut(s) | 676, 1034 |
| BtgZI | GCGATG | 1 cut(s) | 501 |
| BtsCI | GGATG | 6 cut(s) | 271, 509, 645, 1216, 1429, 1829 |
| BtsI | GCAGTG | 2 cut(s) | 750, 1967 |
| BtsIMutI | CAGTG | 5 cut(s) | 750, 874, 1023, 1967, 2079 |
| BveI | ACCTGC | 1 cut(s) | 1316 |
| Cac8I | GCNNGC | 5 cut(s) | 661, 991, 1057, 1314, 2177 |
| CaiI | CAGNNNCTG | 5 cut(s) | 704, 1331, 1682, 2054, 2078 |
| CciI | TCATGA | 1 cut(s) | 1779 |
| CfoI | GCGC | 1 cut(s) | 1659 |
| Cfr9I | CCCGGG | 1 cut(s) | 1010 |
| Csp6I | GTAC | 4 cut(s) | 97, 586, 790, 2126 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 502, 537 |
| CviQI | GTAC | 4 cut(s) | 97, 586, 790, 2126 |
| DdeI | CTNAG | 8 cut(s) | 100, 282, 639, 985, 1149, 1185, 1361, 2229 |
| DpnI | GATC | 9 cut(s) | 141, 181, 575, 766, 935, 1068, 1386, 1641, 2058 |
| DpnII | GATC | 9 cut(s) | 139, 179, 573, 764, 933, 1066, 1384, 1639, 2056 |
| DriI | GACNNNNNGTC | 1 cut(s) | 130 |
| EaeI | YGGCCR | 3 cut(s) | 464, 673, 803 |
| Eam1104I | CTCTTC | 6 cut(s) | 50, 1210, 1540, 1743, 2083, 2193 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 130 |
| EarI | CTCTTC | 6 cut(s) | 50, 1210, 1540, 1743, 2083, 2193 |
| Eco105I | TACGTA | 1 cut(s) | 2122 |
| Eco130I | CCWWGG | 2 cut(s) | 676, 2007 |
| Eco24I | GRGCYC | 3 cut(s) | 208, 1757, 2303 |
| Eco32I | GATATC | 1 cut(s) | 1582 |
| Eco47I | GGWCC | 6 cut(s) | 124, 295, 500, 945, 1544, 1894 |
| Eco57I | CTGAAG | 2 cut(s) | 466, 2243 |
| Eco81I | CCTNAGG | 1 cut(s) | 2229 |
| Eco88I | CYCGRG | 2 cut(s) | 718, 1010 |
| EcoO109I | RGGNCCY | 5 cut(s) | 204, 295, 945, 1001, 1544 |
| EcoRII | CCWGG | 6 cut(s) | 120, 1236, 1591, 1897, 2110, 2167 |
| EcoRV | GATATC | 1 cut(s) | 1582 |
| EcoT14I | CCWWGG | 2 cut(s) | 676, 2007 |
| EcoT38I | GRGCYC | 3 cut(s) | 208, 1757, 2303 |
| ErhI | CCWWGG | 2 cut(s) | 676, 2007 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 588, 620, 1305, 1337 |
| FaqI | GGGAC | 4 cut(s) | 701, 1027, 1557, 1989 |
| FauNDI | CATATG | 1 cut(s) | 610 |
| FbaI | TGATCA | 1 cut(s) | 1639 |
| FblI | GTMKAC | 1 cut(s) | 1454 |
| FokI | GGATG | 6 cut(s) | 278, 496, 632, 1223, 1436, 1816 |
| FriOI | GRGCYC | 3 cut(s) | 208, 1757, 2303 |
| FspBI | CTAG | 4 cut(s) | 1056, 1539, 2085, 2323 |
| GlaI | GCGC | 1 cut(s) | 1658 |
| GsaI | CCCAGC | 1 cut(s) | 1219 |
| GsuI | CTGGAG | 4 cut(s) | 1063, 1155, 1641, 2094 |
| HaeII | RGCGCY | 1 cut(s) | 1660 |
| HaeIII | GGCC | 6 cut(s) | 206, 466, 675, 805, 1002, 1633 |
| HapII | CCGG | 2 cut(s) | 467, 1011 |
| HhaI | GCGC | 1 cut(s) | 1659 |
| Hin6I | GCGC | 1 cut(s) | 1657 |
| HinP1I | GCGC | 1 cut(s) | 1657 |
| HincII | GTYRAC | 1 cut(s) | 696 |
| HindII | GTYRAC | 1 cut(s) | 696 |
| HinfI | GANTC | 4 cut(s) | 470, 525, 1679, 1826 |
| HpaII | CCGG | 2 cut(s) | 467, 1011 |
| HphI | GGTGA | 5 cut(s) | 759, 917, 1023, 1733, 2207 |
| Hpy166II | GTNNAC | 4 cut(s) | 97, 625, 696, 1455 |
| Hpy8I | GTNNAC | 4 cut(s) | 97, 625, 696, 1455 |
| Hpy99I | CGWCG | 1 cut(s) | 136 |
| HpyAV | CCTTC | 9 cut(s) | 138, 406, 485, 958, 958, 1243, 1622, 1804, 2049 |
| HpyCH4IV | ACGT | 1 cut(s) | 2121 |
| HpyF3I | CTNAG | 8 cut(s) | 100, 282, 639, 985, 1149, 1185, 1361, 2229 |
| HpySE526I | ACGT | 1 cut(s) | 2121 |
| HspAI | GCGC | 1 cut(s) | 1657 |
| Ksp22I | TGATCA | 1 cut(s) | 1639 |
| Kzo9I | GATC | 9 cut(s) | 139, 179, 573, 764, 933, 1066, 1384, 1639, 2056 |
| LweI | GCATC | 5 cut(s) | 823, 847, 1117, 1171, 1201 |
| MaeI | CTAG | 4 cut(s) | 1056, 1539, 2085, 2323 |
| MaeII | ACGT | 1 cut(s) | 2121 |
| MaeIII | GTNAC | 6 cut(s) | 151, 392, 869, 1029, 2074, 2080 |
| MalI | GATC | 9 cut(s) | 141, 181, 575, 766, 935, 1068, 1386, 1641, 2058 |
| MboI | GATC | 9 cut(s) | 139, 179, 573, 764, 933, 1066, 1384, 1639, 2056 |
| MfeI | CAATTG | 1 cut(s) | 1062 |
| MhlI | GDGCHC | 5 cut(s) | 109, 208, 1757, 2245, 2303 |
| MlsI | TGGCCA | 1 cut(s) | 675 |
| MluNI | TGGCCA | 1 cut(s) | 675 |
| MmeI | TCCRAC | 3 cut(s) | 1030, 1315, 2010 |
| Mox20I | TGGCCA | 1 cut(s) | 675 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2207 |
| MscI | TGGCCA | 1 cut(s) | 675 |
| MseI | TTAA | 8 cut(s) | 1350, 1478, 1572, 1767, 1916, 2031, 2094, 2133 |
| MslI | CAYNNNNRTG | 2 cut(s) | 514, 1999 |
| Msp20I | TGGCCA | 1 cut(s) | 675 |
| MspA1I | CMGCKG | 2 cut(s) | 701, 989 |
| MspCI | CTTAAG | 1 cut(s) | 1571 |
| MspI | CCGG | 2 cut(s) | 467, 1011 |
| MspR9I | CCNGG | 8 cut(s) | 122, 1011, 1012, 1238, 1593, 1899, 2112, 2169 |
| MunI | CAATTG | 1 cut(s) | 1062 |
| Mva1269I | GAATGC | 1 cut(s) | 1275 |
| MvaI | CCWGG | 6 cut(s) | 122, 1238, 1593, 1899, 2112, 2169 |
| MvnI | CGCG | 1 cut(s) | 663 |
| NciI | CCSGG | 2 cut(s) | 1011, 1012 |
| NcoI | CCATGG | 1 cut(s) | 676 |
| NdeI | CATATG | 1 cut(s) | 610 |
| NdeII | GATC | 9 cut(s) | 139, 179, 573, 764, 933, 1066, 1384, 1639, 2056 |
| NheI | GCTAGC | 1 cut(s) | 1055 |
| NlaIV | GGNNCC | 9 cut(s) | 126, 206, 297, 946, 1545, 1754, 1896, 2139, 2300 |
| NmuCI | GTSAC | 4 cut(s) | 392, 869, 1029, 2074 |
| NspI | RCATGY | 3 cut(s) | 1412, 1874, 2004 |
| PagI | TCATGA | 1 cut(s) | 1779 |
| PaqCI | CACCTGC | 1 cut(s) | 1316 |
| PciI | ACATGT | 3 cut(s) | 1408, 1870, 2000 |
| PctI | GAATGC | 1 cut(s) | 1275 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2207 |
| PfeI | GAWTC | 4 cut(s) | 470, 525, 1679, 1826 |
| PflFI | GACNNNGTC | 1 cut(s) | 1018 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 815 |
| PfoI | TCCNGGA | 1 cut(s) | 120 |
| Ppu21I | YACGTR | 1 cut(s) | 2122 |
| PpuMI | RGGWCCY | 3 cut(s) | 295, 945, 1544 |
| PscI | ACATGT | 3 cut(s) | 1408, 1870, 2000 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1305 |
| Psp5II | RGGWCCY | 3 cut(s) | 295, 945, 1544 |
| Psp6I | CCWGG | 6 cut(s) | 120, 1236, 1591, 1897, 2110, 2167 |
| PspFI | CCCAGC | 1 cut(s) | 1215 |
| PspGI | CCWGG | 6 cut(s) | 120, 1236, 1591, 1897, 2110, 2167 |
| PspN4I | GGNNCC | 9 cut(s) | 126, 206, 297, 946, 1545, 1754, 1896, 2139, 2300 |
| PspOMI | GGGCCC | 1 cut(s) | 204 |
| PspPPI | RGGWCCY | 3 cut(s) | 295, 945, 1544 |
| PstI | CTGCAG | 2 cut(s) | 637, 1762 |
| PstNI | CAGNNNCTG | 5 cut(s) | 704, 1331, 1682, 2054, 2078 |
| PsyI | GACNNNGTC | 1 cut(s) | 1018 |
| PvuII | CAGCTG | 2 cut(s) | 701, 989 |
| RsaI | GTAC | 4 cut(s) | 98, 587, 791, 2127 |
| RsaNI | GTAC | 4 cut(s) | 97, 586, 790, 2126 |
| RseI | CAYNNNNRTG | 2 cut(s) | 514, 1999 |
| SaqAI | TTAA | 8 cut(s) | 1350, 1478, 1572, 1767, 1916, 2031, 2094, 2133 |
| Sau3AI | GATC | 9 cut(s) | 139, 179, 573, 764, 933, 1066, 1384, 1639, 2056 |
| ScrFI | CCNGG | 8 cut(s) | 122, 1011, 1012, 1238, 1593, 1899, 2112, 2169 |
| SduI | GDGCHC | 5 cut(s) | 109, 208, 1757, 2245, 2303 |
| SfaNI | GCATC | 5 cut(s) | 823, 847, 1117, 1171, 1201 |
| SfcI | CTRYAG | 4 cut(s) | 633, 975, 1758, 1785 |
| SinI | GGWCC | 6 cut(s) | 124, 295, 500, 945, 1544, 1894 |
| SmaI | CCCGGG | 1 cut(s) | 1012 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 514, 1999 |
| SmlI | CTYRAG | 3 cut(s) | 1112, 1571, 2244 |
| SmoI | CTYRAG | 3 cut(s) | 1112, 1571, 2244 |
| SnaBI | TACGTA | 1 cut(s) | 2122 |
| SsiI | CCGC | 2 cut(s) | 806, 912 |
| SspI | AATATT | 1 cut(s) | 219 |
| SspMI | CTAG | 4 cut(s) | 1056, 1539, 2085, 2323 |
| StyD4I | CCNGG | 8 cut(s) | 120, 1009, 1010, 1236, 1591, 1897, 2110, 2167 |
| StyI | CCWWGG | 2 cut(s) | 676, 2007 |
| TaiI | ACGT | 1 cut(s) | 2124 |
| TaqI | TCGA | 7 cut(s) | 138, 144, 401, 1022, 1279, 1650, 1691 |
| TaqII | GACCGA | 2 cut(s) | 124, 2149 |
| TatI | WGTACW | 2 cut(s) | 96, 2125 |
| TauI | GCSGC | 2 cut(s) | 808, 914 |
| TfiI | GAWTC | 4 cut(s) | 470, 525, 1679, 1826 |
| Tru1I | TTAA | 8 cut(s) | 1350, 1478, 1572, 1767, 1916, 2031, 2094, 2133 |
| Tru9I | TTAA | 8 cut(s) | 1350, 1478, 1572, 1767, 1916, 2031, 2094, 2133 |
| TscAI | CASTG | 5 cut(s) | 757, 874, 1023, 1974, 2079 |
| TseFI | GTSAC | 4 cut(s) | 392, 869, 1029, 2074 |
| Tsp45I | GTSAC | 4 cut(s) | 392, 869, 1029, 2074 |
| TspDTI | ATGAA | 7 cut(s) | 59, 213, 342, 504, 1386, 1518, 1818 |
| TspGWI | ACGGA | 2 cut(s) | 396, 1508 |
| TspMI | CCCGGG | 1 cut(s) | 1010 |
| TspRI | CASTG | 5 cut(s) | 757, 874, 1023, 1974, 2079 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1018 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 815 |
| Vha464I | CTTAAG | 1 cut(s) | 1571 |
| VpaK11BI | GGWCC | 6 cut(s) | 124, 295, 500, 945, 1544, 1894 |
| XapI | RAATTY | 4 cut(s) | 82, 1263, 1936, 2207 |
| XceI | RCATGY | 3 cut(s) | 1412, 1874, 2004 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1465 |
| XmaI | CCCGGG | 1 cut(s) | 1010 |
| XmiI | GTMKAC | 1 cut(s) | 1454 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2207 |
| XspI | CTAG | 4 cut(s) | 1056, 1539, 2085, 2323 |