RLG00000036542

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000923
Physical Location & Seq
Reverse (-)
81721135 .. 81729729
8595 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036542

Sequence Viewer

Length: 2508 bp
ATGGAGTGCGCCCCCGCCGATCCTTTCGTCGGCGCCGTGTGCCTGAAATCGAGGAGGACTGAGGTGTTGTTGCGGGACGAATCGGCGAGCGAAGTCGGTCGTTTTAGAGAGATTTCAAATTCTAATTTTGCAAAGTTTTCGAATTTGAATTACAATTTTGTGGTGCCATCGGTGCCGCGGCGGAGGAAGAGGCCAACGCCGGCCGATGCAGCCAAACAGGCATGCCTTGATAGAATTATGAATTTGAGATTGTCTTACATTGTGTACTTGGGAACACATTCCCATGGCCTGAACCCTACGTCTGTAGAACTCGAAGCGGCAACGAATTCTCAGTACAATCTGCTTGGAACTGTTTTGGGAAGCAATGAGAGGGCCCAAGAAGCATTGTTTTACTCCTATACTAGGGAGATCAATGGATTTGCTGCAGTTTTAGAAGAAGAAGAGGCTTCACAGATTGCAAAGGATCCAAATGTTGTGTCTGTTTTTCCAAACAGAAAATTAAAACTACATACAACTCGTTCATGGGAGTTCATGGGACTAGAAGACCAAAATGGAGTACCTCGTCACGGATCGATTTGGAAGAAAGCAAAGTTCGGTGAAAATACAATCATTGGAAACCTTGACACTGGTGTCTGGCCGGAATCACCGAGCTTTAGCGATGAAGGGTTTGGACCAATCCCATCTAAGTGGCGTGGAACTTGTCAGCTTGACACCAAAGACAAAGTTCGTTGCAACAGGAAGCTGATCGGAGCAAGGTACTTTAACAAAGGTTACTTGGAGTCTGCCAGGCTCAGTAATTCCACCATTCCACCTGATGTTCTGGTGTCGCCGCTTGACCAGGAAGGCCATGGCTCACACACCCTTTCAACAGCTGGTGGCATTTATGTGCCGGGGGCTAATGTTTTTGGTCATGGCAATGGCACTGCTAAAGGTGGATCCCCGAAAGCTCGTGTGGCTGCTTACAAAGTATGTTGGCCAAGCTTCGGCAGTACAGAAGGAGGGTGTACGAATGCAGACATCATGGCCGCCTTTGATGCTGCAATCGGTGACGGTGTTGATGTTCTTTCGCTGTCCATTGGTGGTGATCCTGTAGAGTTTTTTGAGGATGGGATCGCAATAGGGTCATTCCATGCGGCTAAGCATGGAATCGTTGTTGTTTGCTCAGCTGGCAATTCAGGACCTGCCCGAGGGACAGTGTCTAATGTGGCACCCTGGATATTAACAGTTGGAGCTAGCACCATTGATCGCGAGTTCACTAGTTATTTATCCTTTGGAAAGGGGAAGCATTTTAAGGGAAAAAGTCTTTCCTCTGGAAGTTTGCCATCTAGGAAGTTTTATCCTGTGATAAGTGGAGCCAATGCTAATGCAGCTACTGCGGTTCCTGAAGATTCCTTGCTATGTTTGAATGGAACCCTTGATCCAAAGAAGGTGAAGGGGAAAATCTTGGTCTGCCTACGCGGGCAGAATGGGAGACCTGAAAAAGCCCAGATAGCTGCTCGTGCCGGCGCTGTTGGAATGGTTTTGGTTAACGATGAGGAAAGTGGAGATGATCTTATGGCTGATCCTTATGTGCTCCCCGCTACACATCTGACTTACACTGATGGAAAACGTGTCTTTGAGTACCTGAATTCTACGAAGAACCCTTTTGGTCACGTTACTCCTGTCAAGACGGTAGAAGGAACAAAGCCAGCTCCATTTATGGCTTCATTCTCATCTCGTGGACCGAGTACCATTGAGCCATCAATCCTTAAGCCTGATATCACAGCACCAGGAGTGGATATAATTGCTGCTTATACTGGAGCACCTCTCCCAACTGATTCAGACCTTGGTAAGCGTCGCCCACTTTACATCACAGAATCTGGAACATCTATGTCATGCCCTCATGTATCTGGAATAGTCGGTCTTCTGAAAACACTTTACCCGTCATGGAGCCCCGCTGCTATTAAGTCTGCAATCTTGACCACAGCGAGAAGACGCGACAACAAACGGGAGTCAATTCTTGATTCAACCAAGGTCTCAGCTACACCACTTGACTATGGTGCAGGCCATGTTAATCCAAATCGCGCAGCAAACCCGGGACTTGTCTATGACTTGACTACCGATGATTACTTGAATTTCTTATGTGCTCGTGGATACAATGCATCATTTCTCAAAATATTTTCCGACAAGCCACACAAATGTTCCAACACCTCTAGCATTATTGCTGATTTAAACTATCCTACAATTGCTGTTCCTGATCTCCAAGACAAACCAATTACCATTTCTAGAAAAGTGAAGAATGTCGGTACTCCAGGCACATATGTAGCATATGTTCGTGCCCCAGATAGAGTTTCTGTTTCGGTTCAGCCTAGCAGGTTGAAATTCAAGTCCATTGGTGAAGAGAAGGAATTCAAGGTTACGCTGAAGGCAGATGGTAAGGTTCGAAATCCAGACTATGTGTTTGGAGAGTTGAAATGGTCGGATGGCAAGCACACTGTGAGGAGTCCTATTGCTGTGATGCGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

836

Amino Acids

90.03

Weight (kDa)

9.05

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 85 - 170 7.5e-17 Peptidase inhibitor I9
MORF_dom PF21864 113 - 169 1.1e-06 Multiple organellar RNA editing factor 1, MORF domain
Peptidase_S8 PF00082 199 - 661 1.2e-39 Subtilase family
PA PF02225 463 - 537 1.1e-13 PA domain
fn3_6 PF17766 736 - 832 3e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 629
Acc36I ACCTGC 2 cut(s) 1189, 2343
AccB1I GGYRCC 4 cut(s) 32, 163, 172, 1207
AccII CGCG 5 cut(s) 178, 1248, 1458, 1977, 2064
AcoI YGGCCR 4 cut(s) 201, 635, 974, 1023
AcsI RAATTY 8 cut(s) 118, 142, 241, 325, 1627, 2113, 2360, 2387
AcuI CTGAAG 2 cut(s) 1404, 2423
AcyI GRCGYC 1 cut(s) 33
AdeI CACNNNGTG 1 cut(s) 2476
AfaI GTAC 9 cut(s) 266, 335, 558, 758, 991, 1006, 1622, 1729, 2287
AfiI CCNNNNNNNGG 5 cut(s) 29, 402, 566, 983, 1187
AflII CTTAAG 1 cut(s) 1748
AflIII ACRYGT 1 cut(s) 1609
AhlI ACTAGT 1 cut(s) 1256
AjnI CCWGG 5 cut(s) 785, 837, 1211, 1768, 2290
AjuI GAANNNNNNNTTGG 2 cut(s) 2245, 2277
AloI GAACNNNNNNTCC 2 cut(s) 1402, 1434
Alw21I GWGCWC 3 cut(s) 1575, 1804, 2128
Alw26I GTCTC 2 cut(s) 1465, 2020
AlwNI CAGNNNCTG 3 cut(s) 1181, 1373, 1859
Ama87I CYCGRG 2 cut(s) 1185, 2074
AoxI GGCC 9 cut(s) 191, 201, 286, 372, 635, 844, 974, 1023, 2044
ApaI GGGCCC 1 cut(s) 376
ApeKI GCWGC 9 cut(s) 209, 422, 956, 1037, 1367, 1493, 1787, 1937, 2066
ApoI RAATTY 8 cut(s) 118, 142, 241, 325, 1627, 2113, 2360, 2387
ArsI GACNNNNNNTTYG 4 cut(s) 461, 493, 2423, 2455
Asp700I GAANNNNTTC 2 cut(s) 277, 2387
AspLEI GCGC 4 cut(s) 11, 35, 1508, 2066
AspS9I GGNCC 5 cut(s) 372, 373, 671, 1178, 1721
AsuC2I CCSGG 3 cut(s) 891, 2075, 2076
AsuHPI GGTGA 6 cut(s) 608, 636, 1058, 1094, 1441, 2387
AsuII TTCGAA 2 cut(s) 140, 2422
AsuNHI GCTAGC 1 cut(s) 1232
AvaI CYCGRG 2 cut(s) 1185, 2074
AvaII GGWCC 3 cut(s) 671, 1178, 1721
BaeGI GKGCMC 2 cut(s) 376, 2320
BalI TGGCCA 1 cut(s) 976
BamHI GGATCC 2 cut(s) 463, 935
BanI GGYRCC 4 cut(s) 32, 163, 172, 1207
BanII GRGCYC 2 cut(s) 376, 1934
BauI CACGAG 4 cut(s) 948, 1497, 1716, 2127
BbsI GAAGAC 3 cut(s) 549, 1895, 1978
Bbv12I GWGCWC 3 cut(s) 1575, 1804, 2128
BbvI GCAGC 9 cut(s) 221, 409, 943, 1024, 1379, 1480, 1774, 1924, 2078
BccI CCATC 8 cut(s) 175, 688, 1100, 1330, 1595, 1747, 2405, 2456
BceAI ACGGC 1 cut(s) 20
BcgI CGANNNNNNTGC 2 cut(s) 120, 154
BciT130I CCWGG 5 cut(s) 787, 839, 1213, 1770, 2292
BciVI GTATCC 1 cut(s) 2126
BcnI CCSGG 3 cut(s) 891, 2075, 2076
BcoDI GTCTC 2 cut(s) 1465, 2020
BcuI ACTAGT 1 cut(s) 1256
BfaI CTAG 9 cut(s) 402, 539, 1233, 1257, 1326, 2193, 2265, 2349, 2506
BfmI CTRYAG 3 cut(s) 303, 423, 1089
BfoI RGCGCY 2 cut(s) 36, 1509
BfrI CTTAAG 1 cut(s) 1748
BfuAI ACCTGC 2 cut(s) 1189, 2343
BfuI GTATCC 1 cut(s) 2126
BglI GCCNNNNNGGC 1 cut(s) 218
BlpI GCTNAGC 2 cut(s) 1137, 1162
Bme1390I CCNGG 8 cut(s) 787, 839, 891, 1213, 1770, 2075, 2076, 2292
Bme18I GGWCC 3 cut(s) 671, 1178, 1721
BmeT110I CYCGRG 2 cut(s) 1185, 2074
BmgT120I GGNCC 5 cut(s) 372, 373, 671, 1178, 1721
BmrFI CCNGG 8 cut(s) 787, 839, 891, 1213, 1770, 2075, 2076, 2292
BmsI GCATC 4 cut(s) 196, 1024, 2150, 2487
BmtI GCTAGC 1 cut(s) 1236
BpiI GAAGAC 3 cut(s) 549, 1895, 1978
BplI GAGNNNNNCTC 2 cut(s) 1791, 1823
BpmI CTGGAG 2 cut(s) 1818, 2274
Bpu1102I GCTNAGC 2 cut(s) 1137, 1162
Bpu14I TTCGAA 2 cut(s) 140, 2422
BpuMI CCSGG 3 cut(s) 891, 2075, 2076
Bsa29I ATCGAT 1 cut(s) 572
BsaHI GRCGYC 1 cut(s) 33
BsaI GGTCTC 2 cut(s) 1465, 2020
BsaJI CCNNGG 9 cut(s) 176, 283, 847, 890, 1186, 1211, 1825, 2010, 2074
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 402, 566, 983, 1187
Bse118I RCCGGY 2 cut(s) 199, 1502
Bse1I ACTGG 2 cut(s) 631, 1801
Bse3DI GCAATG 2 cut(s) 370, 922
BseBI CCWGG 5 cut(s) 787, 839, 1213, 1770, 2292
BseCI ATCGAT 1 cut(s) 572
BseDI CCNNGG 9 cut(s) 176, 283, 847, 890, 1186, 1211, 1825, 2010, 2074
BseGI GGATG 2 cut(s) 1111, 2467
BseLI CCNNNNNNNGG 5 cut(s) 29, 402, 566, 983, 1187
BseMI GCAATG 2 cut(s) 370, 922
BseMII CTCAG 5 cut(s) 51, 344, 805, 1176, 2031
BseNI ACTGG 2 cut(s) 631, 1801
BseRI GAGGAG 2 cut(s) 67, 2494
BseSI GKGCMC 2 cut(s) 376, 2320
BseX3I CGGCCG 1 cut(s) 201
BseXI GCAGC 9 cut(s) 221, 409, 943, 1024, 1379, 1480, 1774, 1924, 2078
BsgI GTGCAG 1 cut(s) 2061
Bsh1236I CGCG 5 cut(s) 178, 1248, 1458, 1977, 2064
Bsh1285I CGRYCG 2 cut(s) 100, 204
BshFI GGCC 9 cut(s) 193, 203, 288, 374, 637, 846, 976, 1025, 2046
BshNI GGYRCC 4 cut(s) 32, 163, 172, 1207
BshVI ATCGAT 1 cut(s) 572
BsiEI CGRYCG 2 cut(s) 100, 204
BsiHKAI GWGCWC 3 cut(s) 1575, 1804, 2128
BsiHKCI CYCGRG 2 cut(s) 1185, 2074
BsiSI CCGG 5 cut(s) 200, 638, 890, 1503, 2075
BslFI GGGAC 4 cut(s) 89, 549, 1204, 2091
BslI CCNNNNNNNGG 5 cut(s) 29, 402, 566, 983, 1187
BsmAI GTCTC 2 cut(s) 1465, 2020
BsmFI GGGAC 4 cut(s) 89, 549, 1204, 2091
BsmI GAATGC 1 cut(s) 1015
BsnI GGCC 9 cut(s) 193, 203, 288, 374, 637, 846, 976, 1025, 2046
Bso31I GGTCTC 2 cut(s) 1465, 2020
BsoBI CYCGRG 2 cut(s) 1185, 2074
Bsp119I TTCGAA 2 cut(s) 140, 2422
Bsp120I GGGCCC 1 cut(s) 372
Bsp1286I GDGCHC 6 cut(s) 376, 1575, 1804, 1934, 2128, 2320
Bsp1720I GCTNAGC 2 cut(s) 1137, 1162
Bsp19I CCATGG 2 cut(s) 283, 847
Bsp68I TCGCGA 1 cut(s) 1248
BspANI GGCC 9 cut(s) 193, 203, 288, 374, 637, 846, 976, 1025, 2046
BspCNI CTCAG 5 cut(s) 52, 343, 804, 1175, 2030
BspDI ATCGAT 1 cut(s) 572
BspFNI CGCG 5 cut(s) 178, 1248, 1458, 1977, 2064
BspMAI CTGCAG 1 cut(s) 427
BspMI ACCTGC 2 cut(s) 1189, 2343
BspOI GCTAGC 1 cut(s) 1236
BspT104I TTCGAA 2 cut(s) 140, 2422
BspT107I GGYRCC 4 cut(s) 32, 163, 172, 1207
BspTI CTTAAG 1 cut(s) 1748
BspTNI GGTCTC 2 cut(s) 1465, 2020
BsrDI GCAATG 2 cut(s) 370, 922
BsrFI RCCGGY 2 cut(s) 199, 1502
BsrI ACTGG 2 cut(s) 631, 1801
BssAI RCCGGY 2 cut(s) 199, 1502
BssECI CCNNGG 9 cut(s) 176, 283, 847, 890, 1186, 1211, 1825, 2010, 2074
BssNI GRCGYC 1 cut(s) 33
BssSI CACGAG 4 cut(s) 948, 1497, 1716, 2127
BssT1I CCWWGG 4 cut(s) 283, 847, 1825, 2010
Bst2BI CACGAG 4 cut(s) 948, 1497, 1716, 2127
Bst2UI CCWGG 5 cut(s) 787, 839, 1213, 1770, 2292
Bst4CI ACNGT 6 cut(s) 352, 1052, 1195, 1225, 1672, 2476
Bst6I CTCTTC 3 cut(s) 182, 435, 2373
BstACI GRCGYC 1 cut(s) 33
BstAFI CTTAAG 1 cut(s) 1748
BstAPI GCANNNNNTGC 1 cut(s) 1373
BstBI TTCGAA 2 cut(s) 140, 2422
BstDEI CTNAG 7 cut(s) 60, 330, 684, 791, 1137, 1162, 2017
BstDSI CCRYGG 3 cut(s) 176, 283, 847
BstENI CCTNNNNNAGG 2 cut(s) 400, 1185
BstF5I GGATG 2 cut(s) 1111, 2467
BstFNI CGCG 5 cut(s) 178, 1248, 1458, 1977, 2064
BstH2I RGCGCY 2 cut(s) 36, 1509
BstHHI GCGC 4 cut(s) 11, 35, 1508, 2066
BstMAI GTCTC 2 cut(s) 1465, 2020
BstMCI CGRYCG 2 cut(s) 100, 204
BstNI CCWGG 5 cut(s) 787, 839, 1213, 1770, 2292
BstNSI RCATGY 1 cut(s) 225
BstSCI CCNGG 8 cut(s) 785, 837, 889, 1211, 1768, 2073, 2074, 2290
BstSFI CTRYAG 3 cut(s) 303, 423, 1089
BstSLI GKGCMC 2 cut(s) 376, 2320
BstUI CGCG 5 cut(s) 178, 1248, 1458, 1977, 2064
BstV1I GCAGC 9 cut(s) 221, 409, 943, 1024, 1379, 1480, 1774, 1924, 2078
BstV2I GAAGAC 3 cut(s) 549, 1895, 1978
BstX2I RGATCY 2 cut(s) 463, 935
BstXI CCANNNNNNTGG 1 cut(s) 687
BstYI RGATCY 2 cut(s) 463, 935
BstZI CGGCCG 1 cut(s) 201
Bsu15I ATCGAT 1 cut(s) 572
BsuI GTATCC 1 cut(s) 2126
BsuRI GGCC 9 cut(s) 193, 203, 288, 374, 637, 846, 976, 1025, 2046
BsuTUI ATCGAT 1 cut(s) 572
BtgI CCRYGG 3 cut(s) 176, 283, 847
BtgZI GCGATG 1 cut(s) 672
BtsCI GGATG 2 cut(s) 1111, 2467
BtsI GCAGTG 1 cut(s) 921
BtsIMutI CAGTG 5 cut(s) 624, 921, 1200, 1596, 2472
BtuMI TCGCGA 1 cut(s) 1248
BveI ACCTGC 2 cut(s) 1189, 2343
CaiI CAGNNNCTG 3 cut(s) 1181, 1373, 1859
CfoI GCGC 4 cut(s) 11, 35, 1508, 2066
Cfr10I RCCGGY 2 cut(s) 199, 1502
Cfr13I GGNCC 5 cut(s) 372, 373, 671, 1178, 1721
Cfr42I CCGCGG 1 cut(s) 179
Cfr9I CCCGGG 1 cut(s) 2074
ClaI ATCGAT 1 cut(s) 572
CseI GACGC 2 cut(s) 1823, 1983
Csp6I GTAC 9 cut(s) 265, 334, 557, 757, 990, 1005, 1621, 1728, 2286
CviQI GTAC 9 cut(s) 265, 334, 557, 757, 990, 1005, 1621, 1728, 2286
DdeI CTNAG 7 cut(s) 60, 330, 684, 791, 1137, 1162, 2017
DinI GGCGCC 1 cut(s) 34
DraI TTTAAA 1 cut(s) 2211
DraIII CACNNNGTG 1 cut(s) 2476
DrdI GACNNNNNNGTC 1 cut(s) 629
DseDI GACNNNNNNGTC 1 cut(s) 629
EaeI YGGCCR 4 cut(s) 201, 635, 974, 1023
EagI CGGCCG 1 cut(s) 201
Eam1104I CTCTTC 3 cut(s) 182, 435, 2373
EarI CTCTTC 3 cut(s) 182, 435, 2373
EciI GGCGGA 1 cut(s) 196
EclXI CGGCCG 1 cut(s) 201
Eco130I CCWWGG 4 cut(s) 283, 847, 1825, 2010
Eco24I GRGCYC 2 cut(s) 376, 1934
Eco31I GGTCTC 2 cut(s) 1465, 2020
Eco32I GATATC 1 cut(s) 1759
Eco47I GGWCC 3 cut(s) 671, 1178, 1721
Eco52I CGGCCG 1 cut(s) 201
Eco57I CTGAAG 2 cut(s) 1404, 2423
Eco88I CYCGRG 2 cut(s) 1185, 2074
EcoNI CCTNNNNNAGG 2 cut(s) 400, 1185
EcoO109I RGGNCCY 2 cut(s) 372, 1178
EcoRI GAATTC 3 cut(s) 325, 1627, 2387
EcoRII CCWGG 5 cut(s) 785, 837, 1211, 1768, 2290
EcoRV GATATC 1 cut(s) 1759
EcoT14I CCWWGG 4 cut(s) 283, 847, 1825, 2010
EcoT22I ATGCAT 1 cut(s) 2143
EcoT38I GRGCYC 2 cut(s) 376, 1934
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
ErhI CCWWGG 4 cut(s) 283, 847, 1825, 2010
FaqI GGGAC 4 cut(s) 89, 549, 1204, 2091
FauI CCCGC 5 cut(s) 22, 66, 1451, 1585, 1942
FauNDI CATATG 2 cut(s) 2299, 2308
FokI GGATG 2 cut(s) 1118, 2474
FriOI GRGCYC 2 cut(s) 376, 1934
FspBI CTAG 9 cut(s) 402, 539, 1233, 1257, 1326, 2193, 2265, 2349, 2506
GlaI GCGC 4 cut(s) 10, 34, 1507, 2065
GsuI CTGGAG 2 cut(s) 1818, 2274
HaeII RGCGCY 2 cut(s) 36, 1509
HaeIII GGCC 9 cut(s) 193, 203, 288, 374, 637, 846, 976, 1025, 2046
HapII CCGG 5 cut(s) 200, 638, 890, 1503, 2075
HgaI GACGC 2 cut(s) 1823, 1983
HhaI GCGC 4 cut(s) 11, 35, 1508, 2066
Hin1I GRCGYC 1 cut(s) 33
Hin6I GCGC 4 cut(s) 9, 33, 1506, 2064
HinP1I GCGC 4 cut(s) 9, 33, 1506, 2064
HincII GTYRAC 1 cut(s) 1528
HindII GTYRAC 1 cut(s) 1528
HindIII AAGCTT 1 cut(s) 979
HpaI GTTAAC 1 cut(s) 1528
HpaII CCGG 5 cut(s) 200, 638, 890, 1503, 2075
HphI GGTGA 6 cut(s) 608, 636, 1058, 1094, 1441, 2387
Hpy166II GTNNAC 5 cut(s) 265, 1005, 1254, 1528, 1721
Hpy188I TCNGA 6 cut(s) 749, 1590, 1822, 1908, 2164, 2461
Hpy8I GTNNAC 5 cut(s) 265, 1005, 1254, 1528, 1721
Hpy99I CGWCG 2 cut(s) 32, 1839
HpyAV CCTTC 8 cut(s) 656, 836, 989, 1420, 1426, 1670, 2377, 2398
HpyCH4III ACNGT 6 cut(s) 352, 1052, 1195, 1225, 1672, 2476
HpyCH4IV ACGT 3 cut(s) 299, 1609, 1653
HpyF3I CTNAG 7 cut(s) 60, 330, 684, 791, 1137, 1162, 2017
HpySE526I ACGT 3 cut(s) 299, 1609, 1653
Hsp92I GRCGYC 1 cut(s) 33
HspAI GCGC 4 cut(s) 9, 33, 1506, 2064
KasI GGCGCC 1 cut(s) 32
KroI GCCGGC 2 cut(s) 199, 1502
KroNI GCCGGC 2 cut(s) 201, 1504
KspAI GTTAAC 1 cut(s) 1528
KspI CCGCGG 1 cut(s) 179
LmnI GCTCC 7 cut(s) 749, 1229, 1352, 1578, 1696, 1799, 1929
Lsp1109I GCAGC 9 cut(s) 221, 409, 943, 1024, 1379, 1480, 1774, 1924, 2078
LweI GCATC 4 cut(s) 196, 1024, 2150, 2487
MaeI CTAG 9 cut(s) 402, 539, 1233, 1257, 1326, 2193, 2265, 2349, 2506
MaeII ACGT 3 cut(s) 299, 1609, 1653
MaeIII GTNAC 7 cut(s) 563, 770, 1046, 1649, 1654, 2395, 2501
MfeI CAATTG 1 cut(s) 2223
MflI RGATCY 2 cut(s) 463, 935
MhlI GDGCHC 6 cut(s) 376, 1575, 1804, 1934, 2128, 2320
MlsI TGGCCA 1 cut(s) 976
MluNI TGGCCA 1 cut(s) 976
Mly113I GGCGCC 1 cut(s) 33
MlyI GAGTC 3 cut(s) 788, 2000, 2491
MmeI TCCRAC 5 cut(s) 1207, 1492, 2187, 2208, 2439
Mox20I TGGCCA 1 cut(s) 976
Mph1103I ATGCAT 1 cut(s) 2143
MroNI GCCGGC 2 cut(s) 199, 1502
MroXI GAANNNNTTC 2 cut(s) 277, 2387
MscI TGGCCA 1 cut(s) 976
MseI TTAA 9 cut(s) 500, 762, 1220, 1290, 1527, 1749, 1944, 2052, 2210
MslI CAYNNNNRTG 5 cut(s) 282, 685, 884, 915, 2176
Msp20I TGGCCA 1 cut(s) 976
MspA1I CMGCKG 4 cut(s) 178, 872, 1166, 1937
MspCI CTTAAG 1 cut(s) 1748
MspI CCGG 5 cut(s) 200, 638, 890, 1503, 2075
MspR9I CCNGG 8 cut(s) 787, 839, 891, 1213, 1770, 2075, 2076, 2292
MunI CAATTG 1 cut(s) 2223
Mva1269I GAATGC 1 cut(s) 1015
MvaI CCWGG 5 cut(s) 787, 839, 1213, 1770, 2292
MvnI CGCG 5 cut(s) 178, 1248, 1458, 1977, 2064
NaeI GCCGGC 2 cut(s) 201, 1504
NarI GGCGCC 1 cut(s) 33
NciI CCSGG 3 cut(s) 891, 2075, 2076
NcoI CCATGG 2 cut(s) 283, 847
NdeI CATATG 2 cut(s) 2299, 2308
NgoMIV GCCGGC 2 cut(s) 199, 1502
NheI GCTAGC 1 cut(s) 1232
NmuCI GTSAC 3 cut(s) 563, 1046, 1649
NruI TCGCGA 1 cut(s) 1248
NsiI ATGCAT 1 cut(s) 2143
NspI RCATGY 1 cut(s) 225
NspV TTCGAA 2 cut(s) 140, 2422
PaeI GCATGC 1 cut(s) 225
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PctI GAATGC 1 cut(s) 1015
PdiI GCCGGC 2 cut(s) 201, 1504
PdmI GAANNNNTTC 2 cut(s) 277, 2387
PfeI GAWTC 7 cut(s) 80, 641, 1146, 1388, 1817, 1856, 2003
PflFI GACNNNGTC 1 cut(s) 1195
PleI GAGTC 3 cut(s) 787, 1999, 2490
PluTI GGCGCC 1 cut(s) 36
PpsI GAGTC 3 cut(s) 787, 1999, 2490
PpuMI RGGWCCY 1 cut(s) 1178
Psp5II RGGWCCY 1 cut(s) 1178
Psp6I CCWGG 5 cut(s) 785, 837, 1211, 1768, 2290
PspGI CCWGG 5 cut(s) 785, 837, 1211, 1768, 2290
PspOMI GGGCCC 1 cut(s) 372
PspPI GGNCC 5 cut(s) 372, 373, 671, 1178, 1721
PspPPI RGGWCCY 1 cut(s) 1178
PsrI GAACNNNNNNTAC 2 cut(s) 2295, 2327
PstI CTGCAG 1 cut(s) 427
PstNI CAGNNNCTG 3 cut(s) 1181, 1373, 1859
PsuI RGATCY 2 cut(s) 463, 935
PsyI GACNNNGTC 1 cut(s) 1195
PvuII CAGCTG 2 cut(s) 872, 1166
RruI TCGCGA 1 cut(s) 1248
RsaI GTAC 9 cut(s) 266, 335, 558, 758, 991, 1006, 1622, 1729, 2287
RsaNI GTAC 9 cut(s) 265, 334, 557, 757, 990, 1005, 1621, 1728, 2286
RseI CAYNNNNRTG 5 cut(s) 282, 685, 884, 915, 2176
SacII CCGCGG 1 cut(s) 179
SaqAI TTAA 9 cut(s) 500, 762, 1220, 1290, 1527, 1749, 1944, 2052, 2210
Sau96I GGNCC 5 cut(s) 372, 373, 671, 1178, 1721
SchI GAGTC 3 cut(s) 788, 2000, 2491
ScrFI CCNGG 8 cut(s) 787, 839, 891, 1213, 1770, 2075, 2076, 2292
SduI GDGCHC 6 cut(s) 376, 1575, 1804, 1934, 2128, 2320
SfaNI GCATC 4 cut(s) 196, 1024, 2150, 2487
SfcI CTRYAG 3 cut(s) 303, 423, 1089
SfoI GGCGCC 1 cut(s) 34
Sfr303I CCGCGG 1 cut(s) 179
SfuI TTCGAA 2 cut(s) 140, 2422
SgrBI CCGCGG 1 cut(s) 179
SinI GGWCC 3 cut(s) 671, 1178, 1721
SmaI CCCGGG 1 cut(s) 2076
SmiMI CAYNNNNRTG 5 cut(s) 282, 685, 884, 915, 2176
SmlI CTYRAG 1 cut(s) 1748
SmoI CTYRAG 1 cut(s) 1748
SpeI ACTAGT 1 cut(s) 1256
SphI GCATGC 1 cut(s) 225
SspDI GGCGCC 1 cut(s) 32
SspI AATATT 1 cut(s) 2157
SspMI CTAG 9 cut(s) 402, 539, 1233, 1257, 1326, 2193, 2265, 2349, 2506
StyD4I CCNGG 8 cut(s) 785, 837, 889, 1211, 1768, 2073, 2074, 2290
StyI CCWWGG 4 cut(s) 283, 847, 1825, 2010
TaaI ACNGT 6 cut(s) 352, 1052, 1195, 1225, 1672, 2476
TaiI ACGT 3 cut(s) 302, 1612, 1656
TaqI TCGA 5 cut(s) 50, 140, 312, 572, 2422
TaqII GACCGA 3 cut(s) 86, 1738, 1889
TatI WGTACW 3 cut(s) 264, 333, 989
TauI GCSGC 6 cut(s) 178, 181, 320, 832, 1028, 1136
TfiI GAWTC 7 cut(s) 80, 641, 1146, 1388, 1817, 1856, 2003
Tru1I TTAA 9 cut(s) 500, 762, 1220, 1290, 1527, 1749, 1944, 2052, 2210
Tru9I TTAA 9 cut(s) 500, 762, 1220, 1290, 1527, 1749, 1944, 2052, 2210
TscAI CASTG 5 cut(s) 631, 928, 1200, 1603, 2479
TseFI GTSAC 3 cut(s) 563, 1046, 1649
TseI GCWGC 9 cut(s) 209, 422, 956, 1037, 1367, 1493, 1787, 1937, 2066
Tsp45I GTSAC 3 cut(s) 563, 1046, 1649
TspDTI ATGAA 5 cut(s) 254, 510, 520, 675, 1695
TspGWI ACGGA 1 cut(s) 582
TspMI CCCGGG 1 cut(s) 2074
TspRI CASTG 5 cut(s) 631, 928, 1200, 1603, 2479
Tth111I GACNNNGTC 1 cut(s) 1195
Vha464I CTTAAG 1 cut(s) 1748
VpaK11BI GGWCC 3 cut(s) 671, 1178, 1721
XagI CCTNNNNNAGG 2 cut(s) 400, 1185
XapI RAATTY 8 cut(s) 118, 142, 241, 325, 1627, 2113, 2360, 2387
XbaI TCTAGA 1 cut(s) 2264
XceI RCATGY 1 cut(s) 225
XcmI CCANNNNNNNNNTGG 1 cut(s) 845
XmaI CCCGGG 1 cut(s) 2074
XmnI GAANNNNTTC 2 cut(s) 277, 2387
XspI CTAG 9 cut(s) 402, 539, 1233, 1257, 1326, 2193, 2265, 2349, 2506
Zsp2I ATGCAT 1 cut(s) 2143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.