FvH4_4g20610

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001085
Physical Location & Seq
Reverse (-)
23767118 .. 23770357
3240 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20610.t1

Sequence Viewer

Length: 1014 bp
ATGGCGGTCCAGTTGTCAGCGGCAGAGAAGAAGACCGGGATTGGAAACCATAGATCTGAACCTTCTCTCTATCCATTCTTCCACTTCGATTCCGCCGCCGCCGCCAGGTCGGTCGGAAATCTCGCCGGAGGTCGGCCGGAAACCTCGCCTGAGGTCTGCCTGAAACCTCGCCAGAGGTCCGCCGGAGACTGTTCCGAAATGAGAAATCTGGCAGACTTTCTACTGGTACCAGAAGCTTGTGAGTCTGGGAGTATGTTTGAGGGTTTGCTTCCAAAGAATATAAACATATATGCTACTACAGCAGCAAATGGAGAGGAGAGTAGTTATGCAACATACTGTTCTGGAGATGTTCCTGAAAAAATCGGTACTTGTTTGGGAGACTTGTATAGTATTTCCTGGTTGGAGGATTGCGACGCACATGATCTGCGCAAAGAGACTTTTGAGGTTCAATATGAAAGGGTTCGGAACAGAACAGATGAATCCCATGTTATGCAATATGGAGATATGAGTTACATAAAGCAGTTCCTGTACACTTATTTGGGGGCAACTTCTAAAGCTCATAGCCATGCTCCCCCTGCCCTCACAGCCTCGCCTTCGGGATCAATCTCAAGGACTGTTAGCCAGCGCGATGCAGCCCTCTTTTATTTTCTGTATAAGGTAAACAGAGCTCCAACTAACTCTCATAAGAAGCTCGAAGCTAATAAGCAGCTATATAATGAAATTGCTCGAAGGAAACGTGTAGATGATAGCATAACCAAAATCGGAGAGCTTCTGTTTGGGACCGAGAAGGGCTCAGAATTACTGATGAAGGTCAGGCCTTCAGGACAACCTCTAGTGGATGACTGGGAATGCTTCAAGATATTAGGACTTATGAGAGTCGCTGCGGAAAGCTGTCGACATATATATGGAATGAAGTACATGCGAGCTTTTGCCAACATGTGCAATGACGGGATCAGCGTAGAGAAACTGGTTGCAGTGTCTGATCAAGCTTGTTCTAAGAAACCTCATGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

338

Amino Acids

37.4

Weight (kDa)

7.09

Isoelectric Point (pI)

50.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 72 - 181 1.7e-37 Peptidase C13 family
Legum_prodom PF20985 235 - 331 1.7e-29 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 428
Acc65I GGTACC 1 cut(s) 226
AccB1I GGYRCC 1 cut(s) 226
AccI GTMKAC 1 cut(s) 895
AccII CGCG 1 cut(s) 627
AciI CCGC 8 cut(s) 5, 20, 93, 96, 99, 102, 180, 884
AclWI GGATC 2 cut(s) 607, 959
AcoI YGGCCR 1 cut(s) 134
AcuI CTGAAG 1 cut(s) 804
AfaI GTAC 4 cut(s) 228, 367, 530, 917
AfiI CCNNNNNNNGG 2 cut(s) 105, 132
AflIII ACRYGT 2 cut(s) 736, 936
AgsI TTSAA 2 cut(s) 449, 856
AjnI CCWGG 2 cut(s) 104, 395
Alw21I GWGCWC 1 cut(s) 670
Alw26I GTCTC 3 cut(s) 180, 372, 428
AlwI GGATC 2 cut(s) 607, 959
AlwNI CAGNNNCTG 2 cut(s) 526, 980
AoxI GGCC 2 cut(s) 134, 815
ApeKI GCWGC 4 cut(s) 302, 632, 706, 881
Asp700I GAANNNNTTC 1 cut(s) 459
Asp718I GGTACC 1 cut(s) 226
AspLEI GCGC 2 cut(s) 429, 627
AspS9I GGNCC 3 cut(s) 7, 177, 780
AsuC2I CCSGG 1 cut(s) 37
AvaII GGWCC 3 cut(s) 7, 177, 780
AxyI CCTNAGG 1 cut(s) 150
BanI GGYRCC 1 cut(s) 226
BanII GRGCYC 2 cut(s) 670, 794
BbsI GAAGAC 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 670
BbvI GCAGC 4 cut(s) 314, 644, 718, 868
BciT130I CCWGG 2 cut(s) 106, 397
BclI TGATCA 1 cut(s) 982
BcnI CCSGG 1 cut(s) 37
BcoDI GTCTC 3 cut(s) 180, 372, 428
BfaI CTAG 1 cut(s) 833
BfmI CTRYAG 1 cut(s) 297
BglII AGATCT 1 cut(s) 53
BisI GCNGC 8 cut(s) 21, 96, 99, 102, 303, 633, 707, 882
BlsI GCNGC 8 cut(s) 22, 97, 100, 103, 304, 634, 708, 883
Bme1390I CCNGG 3 cut(s) 37, 106, 397
Bme18I GGWCC 3 cut(s) 7, 177, 780
BmgT120I GGNCC 3 cut(s) 7, 177, 780
BmiI GGNNCC 2 cut(s) 228, 781
BmrFI CCNGG 3 cut(s) 37, 106, 397
BmrI ACTGGG 1 cut(s) 853
BmsI GCATC 1 cut(s) 619
BmuI ACTGGG 1 cut(s) 853
BpiI GAAGAC 1 cut(s) 38
BplI GAGNNNNNCTC 2 cut(s) 776, 808
BpmI CTGGAG 1 cut(s) 363
BpuEI CTTGAG 1 cut(s) 592
BpuMI CCSGG 1 cut(s) 37
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 132
Bse1I ACTGG 4 cut(s) 10, 228, 848, 972
Bse21I CCTNAGG 1 cut(s) 150
Bse3DI GCAATG 1 cut(s) 949
BseBI CCWGG 2 cut(s) 106, 397
BseGI GGATG 1 cut(s) 844
BseLI CCNNNNNNNGG 2 cut(s) 105, 132
BseMI GCAATG 1 cut(s) 949
BseMII CTCAG 2 cut(s) 141, 807
BseNI ACTGG 4 cut(s) 10, 228, 848, 972
BseRI GAGGAG 1 cut(s) 329
BseX3I CGGCCG 1 cut(s) 134
BseXI GCAGC 4 cut(s) 314, 644, 718, 868
Bsh1236I CGCG 1 cut(s) 627
Bsh1285I CGRYCG 2 cut(s) 114, 137
BshFI GGCC 2 cut(s) 136, 817
BshNI GGYRCC 1 cut(s) 226
BsiEI CGRYCG 2 cut(s) 114, 137
BsiHKAI GWGCWC 1 cut(s) 670
BsiSI CCGG 4 cut(s) 36, 126, 137, 183
BslFI GGGAC 1 cut(s) 793
BslI CCNNNNNNNGG 2 cut(s) 105, 132
BsmAI GTCTC 3 cut(s) 180, 372, 428
BsmFI GGGAC 1 cut(s) 793
BsmI GAATGC 1 cut(s) 854
BsnI GGCC 2 cut(s) 136, 817
Bsp1286I GDGCHC 2 cut(s) 670, 794
Bsp1407I TGTACA 1 cut(s) 528
Bsp143I GATC 5 cut(s) 53, 421, 599, 951, 982
BspACI CCGC 8 cut(s) 5, 20, 93, 96, 99, 102, 180, 884
BspANI GGCC 2 cut(s) 136, 817
BspCNI CTCAG 2 cut(s) 142, 806
BspFNI CGCG 1 cut(s) 627
BspLI GGNNCC 2 cut(s) 228, 781
BspPI GGATC 2 cut(s) 607, 959
BspT107I GGYRCC 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 949
BsrGI TGTACA 1 cut(s) 528
BsrI ACTGG 4 cut(s) 10, 228, 848, 972
BssMI GATC 5 cut(s) 53, 421, 599, 951, 982
Bst2UI CCWGG 2 cut(s) 106, 397
Bst4CI ACNGT 3 cut(s) 191, 338, 616
BstAUI TGTACA 1 cut(s) 528
BstC8I GCNNGC 2 cut(s) 623, 924
BstDEI CTNAG 3 cut(s) 150, 793, 996
BstF5I GGATG 1 cut(s) 844
BstFNI CGCG 1 cut(s) 627
BstHHI GCGC 2 cut(s) 429, 627
BstKTI GATC 5 cut(s) 56, 424, 602, 954, 985
BstMAI GTCTC 3 cut(s) 180, 372, 428
BstMBI GATC 5 cut(s) 53, 421, 599, 951, 982
BstMCI CGRYCG 2 cut(s) 114, 137
BstMWI GCNNNNNNNGC 4 cut(s) 101, 299, 575, 584
BstNI CCWGG 2 cut(s) 106, 397
BstNSI RCATGY 2 cut(s) 922, 940
BstSCI CCNGG 3 cut(s) 35, 104, 395
BstSFI CTRYAG 1 cut(s) 297
BstUI CGCG 1 cut(s) 627
BstV1I GCAGC 4 cut(s) 314, 644, 718, 868
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 1 cut(s) 53
BstYI RGATCY 1 cut(s) 53
BstZI CGGCCG 1 cut(s) 134
Bsu36I CCTNAGG 1 cut(s) 150
BsuRI GGCC 2 cut(s) 136, 817
BtgZI GCGATG 1 cut(s) 642
BtsCI GGATG 1 cut(s) 844
BtsI GCAGTG 1 cut(s) 981
BtsIMutI CAGTG 1 cut(s) 981
Cac8I GCNNGC 2 cut(s) 623, 924
CaiI CAGNNNCTG 2 cut(s) 526, 980
CfoI GCGC 2 cut(s) 429, 627
Cfr13I GGNCC 3 cut(s) 7, 177, 780
CseI GACGC 1 cut(s) 422
Csp6I GTAC 4 cut(s) 227, 366, 529, 916
CviAII CATG 6 cut(s) 419, 485, 566, 919, 937, 1007
CviQI GTAC 4 cut(s) 227, 366, 529, 916
DdeI CTNAG 3 cut(s) 150, 793, 996
DpnI GATC 5 cut(s) 55, 423, 601, 953, 984
DpnII GATC 5 cut(s) 53, 421, 599, 951, 982
EaeI YGGCCR 1 cut(s) 134
EagI CGGCCG 1 cut(s) 134
EciI GGCGGA 2 cut(s) 82, 169
Ecl136II GAGCTC 1 cut(s) 668
EclXI CGGCCG 1 cut(s) 134
Eco147I AGGCCT 1 cut(s) 817
Eco24I GRGCYC 2 cut(s) 670, 794
Eco47I GGWCC 3 cut(s) 7, 177, 780
Eco52I CGGCCG 1 cut(s) 134
Eco53kI GAGCTC 1 cut(s) 668
Eco57I CTGAAG 1 cut(s) 804
Eco81I CCTNAGG 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 104, 395
EcoT38I GRGCYC 2 cut(s) 670, 794
FaeI CATG 6 cut(s) 422, 488, 569, 922, 940, 1010
FaqI GGGAC 1 cut(s) 793
FatI CATG 6 cut(s) 418, 484, 565, 918, 936, 1006
FbaI TGATCA 1 cut(s) 982
FblI GTMKAC 1 cut(s) 895
Fnu4HI GCNGC 8 cut(s) 21, 96, 99, 102, 303, 633, 707, 882
FokI GGATG 1 cut(s) 851
FriOI GRGCYC 2 cut(s) 670, 794
Fsp4HI GCNGC 8 cut(s) 21, 96, 99, 102, 303, 633, 707, 882
FspBI CTAG 1 cut(s) 833
FspI TGCGCA 1 cut(s) 428
GlaI GCGC 2 cut(s) 428, 626
GluI GCNGC 8 cut(s) 21, 96, 99, 102, 303, 633, 707, 882
GsuI CTGGAG 1 cut(s) 363
HaeIII GGCC 2 cut(s) 136, 817
HapII CCGG 4 cut(s) 36, 126, 137, 183
HgaI GACGC 1 cut(s) 422
HhaI GCGC 2 cut(s) 429, 627
Hin1II CATG 6 cut(s) 422, 488, 569, 922, 940, 1010
Hin6I GCGC 2 cut(s) 427, 625
HinP1I GCGC 2 cut(s) 427, 625
HincII GTYRAC 1 cut(s) 896
HindII GTYRAC 1 cut(s) 896
HindIII AAGCTT 2 cut(s) 234, 987
HinfI GANTC 4 cut(s) 89, 242, 479, 876
HpaII CCGG 4 cut(s) 36, 126, 137, 183
Hpy166II GTNNAC 3 cut(s) 531, 661, 896
Hpy188I TCNGA 7 cut(s) 58, 116, 196, 465, 764, 796, 982
Hpy188III TCNNGA 5 cut(s) 342, 353, 597, 822, 856
Hpy8I GTNNAC 3 cut(s) 531, 661, 896
Hpy99I CGWCG 1 cut(s) 416
HpyAV CCTTC 6 cut(s) 72, 603, 723, 781, 802, 828
HpyCH4III ACNGT 3 cut(s) 191, 338, 616
HpyCH4IV ACGT 1 cut(s) 736
HpyCH4V TGCA 5 cut(s) 329, 493, 632, 942, 974
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 299, 575, 584
HpyF3I CTNAG 3 cut(s) 150, 793, 996
HpySE526I ACGT 1 cut(s) 736
Hsp92II CATG 6 cut(s) 422, 488, 569, 922, 940, 1010
HspAI GCGC 2 cut(s) 427, 625
KpnI GGTACC 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 982
Kzo9I GATC 5 cut(s) 53, 421, 599, 951, 982
LmnI GCTCC 2 cut(s) 574, 673
Lsp1109I GCAGC 4 cut(s) 314, 644, 718, 868
LweI GCATC 1 cut(s) 619
MaeI CTAG 1 cut(s) 833
MaeII ACGT 1 cut(s) 736
MaeIII GTNAC 1 cut(s) 509
MalI GATC 5 cut(s) 55, 423, 601, 953, 984
MboI GATC 5 cut(s) 53, 421, 599, 951, 982
MboII GAAGA 3 cut(s) 40, 43, 70
MflI RGATCY 1 cut(s) 53
MhlI GDGCHC 2 cut(s) 670, 794
MluCI AATT 2 cut(s) 720, 797
MlyI GAGTC 2 cut(s) 251, 885
MmeI TCCRAC 3 cut(s) 94, 381, 695
MroXI GAANNNNTTC 1 cut(s) 459
MslI CAYNNNNRTG 2 cut(s) 564, 903
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 4 cut(s) 36, 126, 137, 183
MspR9I CCNGG 3 cut(s) 37, 106, 397
Mva1269I GAATGC 1 cut(s) 854
MvaI CCWGG 2 cut(s) 106, 397
MvnI CGCG 1 cut(s) 627
MwoI GCNNNNNNNGC 4 cut(s) 101, 299, 575, 584
NciI CCSGG 1 cut(s) 37
NdeII GATC 5 cut(s) 53, 421, 599, 951, 982
NlaIII CATG 6 cut(s) 422, 488, 569, 922, 940, 1010
NlaIV GGNNCC 2 cut(s) 228, 781
NsbI TGCGCA 1 cut(s) 428
NspI RCATGY 2 cut(s) 922, 940
PceI AGGCCT 1 cut(s) 817
PciI ACATGT 1 cut(s) 936
PcsI WCGNNNNNNNCGW 2 cut(s) 733, 954
PctI GAATGC 1 cut(s) 854
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 2 cut(s) 89, 479
PkrI GCNGC 8 cut(s) 22, 97, 100, 103, 304, 634, 708, 883
PleI GAGTC 2 cut(s) 250, 884
PpsI GAGTC 2 cut(s) 250, 884
PscI ACATGT 1 cut(s) 936
Psp124BI GAGCTC 1 cut(s) 670
Psp6I CCWGG 2 cut(s) 104, 395
PspGI CCWGG 2 cut(s) 104, 395
PspN4I GGNNCC 2 cut(s) 228, 781
PspPI GGNCC 3 cut(s) 7, 177, 780
PstNI CAGNNNCTG 2 cut(s) 526, 980
PsuI RGATCY 1 cut(s) 53
RsaI GTAC 4 cut(s) 228, 367, 530, 917
RsaNI GTAC 4 cut(s) 227, 366, 529, 916
RseI CAYNNNNRTG 2 cut(s) 564, 903
SacI GAGCTC 1 cut(s) 670
SalI GTCGAC 1 cut(s) 894
SatI GCNGC 8 cut(s) 21, 96, 99, 102, 303, 633, 707, 882
Sau3AI GATC 5 cut(s) 53, 421, 599, 951, 982
Sau96I GGNCC 3 cut(s) 7, 177, 780
SchI GAGTC 2 cut(s) 251, 885
ScrFI CCNGG 3 cut(s) 37, 106, 397
SduI GDGCHC 2 cut(s) 670, 794
SfaNI GCATC 1 cut(s) 619
SfcI CTRYAG 1 cut(s) 297
SinI GGWCC 3 cut(s) 7, 177, 780
SmiMI CAYNNNNRTG 2 cut(s) 564, 903
SmlI CTYRAG 1 cut(s) 607
SmoI CTYRAG 1 cut(s) 607
Sse9I AATT 2 cut(s) 720, 797
SseBI AGGCCT 1 cut(s) 817
SsiI CCGC 8 cut(s) 5, 20, 93, 96, 99, 102, 180, 884
SspMI CTAG 1 cut(s) 833
SstI GAGCTC 1 cut(s) 670
StuI AGGCCT 1 cut(s) 817
StyD4I CCNGG 3 cut(s) 35, 104, 395
TaaI ACNGT 3 cut(s) 191, 338, 616
TaiI ACGT 1 cut(s) 739
TaqI TCGA 4 cut(s) 87, 693, 727, 895
TaqII GACCGA 2 cut(s) 100, 797
TasI AATT 2 cut(s) 720, 797
TatI WGTACW 2 cut(s) 528, 915
TauI GCSGC 4 cut(s) 23, 98, 101, 104
TfiI GAWTC 2 cut(s) 89, 479
TscAI CASTG 1 cut(s) 981
TseI GCWGC 4 cut(s) 302, 632, 706, 881
TspDTI ATGAA 5 cut(s) 468, 492, 732, 821, 926
TspRI CASTG 1 cut(s) 981
VpaK11BI GGWCC 3 cut(s) 7, 177, 780
XceI RCATGY 2 cut(s) 922, 940
XmiI GTMKAC 1 cut(s) 895
XmnI GAANNNNTTC 1 cut(s) 459
XspI CTAG 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.