Prupe.3G025000_v2.0.a1

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001085
Physical Location & Seq
Forward (+)
1830380 .. 1832842
2463 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G025000.1

Sequence Viewer

Length: 1251 bp
ATGCAGGCTAATGTATGCCATGCATACCAAATACTGAAGAAAGGTGGACTGAAAGATGAGAACATCATTTTTTTCATGTATGATGATATTGCGTTCAACCCGGAAAATCCTAGACAAGGTGTCATCATCAACAAACCAAAAGGCCATGATGTTTATAAAGGAGTTCCCAAGGATTATACAGGGGATGACGTTAATGCAGACAATCTTTATGCCGTTATTCTTGCAAACAAAAGTACCCTCAGTGGAGGTAGTGGGAAGGTTCTGCGTAGCGGTCCAAATGACCATATTTTCATATATTACACTGACCATGGTGCTGCAGGATTACTTGGGATGCCTACTGGTACTCCTTCGGTTTTCACAATAGACCTCATAGATGTGCTCAAGAAGAAGCATGCTGCTAAAGGTTACAAAAGCATGGTATTTTACCTTGAAGCTTGCGAGTCTAGGAGTATATTTGAAGGCCTCCTTCCAAAGAATGGGGCCAGTGCCCACCCTGGGCATGCACTGGCTCCGCCTACATCAGCAAATGCAGAAGAGGATGGGTATGCAACATACTGTCCTGGTCAACCTGGTATTCCTGCTGAGTATGATATCTGTTTGGGGGACTTGTTCAGTATTTCCTGGATAGAGGATTGTGACAAAAATGATTTGCGTAAAGAAACATTGGAGAAGCAATATGAAAGGGTTCGAAGAAGAACAAATGATTCTCATGTTATGCAGTATGGAGATATGAGTCACAGAAAGGAGTTCCTCTTAACTTACATGGGTGCAGATCCTGCAAATGATAGCTACACTTCCATGGAAGATATCTCTTCACCATCAATCTCAAGGGTTGTTAACCAACGTGATGCAGACCTCCTTCATCTTCATCACAAGTTCCACAAAGCTCCTACTGGCTCTCATACCAAACTGGAAGCCCGAAAGCAGCTGCTCGATGAAATTGCTCATAGGAAACTTGTGGACTACAGAATAAATAAAAAAATAGGGGAGCTTTTGTTTGGACATCAGAAGAGCTCAAAAGTGTTGCTTAATGTTAGGCCTCATGGGCAACCTCTAGTAGATGATTGGGATTGCTTCAAGATGCTTGAAAGTCCTTCTCCTATGAGGACTTACGAGAACAACTGTGGACCTTTATCAAATTATGGAATGAAATACGCACGAGCTATTGCCAACATGGCCAACGCTGGGGTAGCCATAGAGAATATGGCTGCAGCATCTGATCAAACTTGTTCTAAGAAACCCGATGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

417

Amino Acids

46.24

Weight (kDa)

6.63

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 156
AccB7I CCANNNNNTGG 1 cut(s) 476
AciI CCGC 2 cut(s) 270, 512
AclWI GGATC 1 cut(s) 767
AcoI YGGCCR 1 cut(s) 1176
AcuI CTGAAG 1 cut(s) 56
AfaI GTAC 2 cut(s) 235, 343
AfiI CCNNNNNNNGG 4 cut(s) 116, 476, 495, 1185
AgsI TTSAA 5 cut(s) 97, 431, 458, 1078, 1088
AhdI GACNNNNNGTC 1 cut(s) 119
AjnI CCWGG 4 cut(s) 493, 559, 568, 620
AluBI AGCT 7 cut(s) 434, 789, 887, 928, 991, 1014, 1163
AluI AGCT 7 cut(s) 434, 789, 887, 928, 991, 1014, 1163
Alw21I GWGCWC 2 cut(s) 381, 1016
AlwI GGATC 1 cut(s) 767
AlwNI CAGNNNCTG 2 cut(s) 776, 1217
AoxI GGCC 5 cut(s) 142, 460, 480, 1037, 1176
ApeKI GCWGC 6 cut(s) 314, 395, 925, 928, 1208, 1211
Asp700I GAANNNNTTC 1 cut(s) 684
AspS9I GGNCC 3 cut(s) 272, 480, 1127
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 1 cut(s) 807
AsuII TTCGAA 1 cut(s) 688
AvaII GGWCC 2 cut(s) 272, 1127
BaeGI GKGCMC 1 cut(s) 490
BaeI ACNNNNGTAYC 2 cut(s) 217, 250
BalI TGGCCA 1 cut(s) 1178
BanII GRGCYC 1 cut(s) 1016
BauI CACGAG 1 cut(s) 1158
Bbv12I GWGCWC 2 cut(s) 381, 1016
BbvI GCAGC 6 cut(s) 301, 382, 915, 937, 1195, 1223
BccI CCATC 2 cut(s) 533, 826
BceAI ACGGC 1 cut(s) 197
BcgI CGANNNNNNTGC 2 cut(s) 923, 957
BciT130I CCWGG 4 cut(s) 495, 561, 570, 622
BclI TGATCA 1 cut(s) 1219
BcnI CCSGG 1 cut(s) 101
BfaI CTAG 4 cut(s) 111, 444, 1055, 1249
BfmI CTRYAG 3 cut(s) 315, 964, 1209
BglI GCCNNNNNGGC 2 cut(s) 1045, 1175
BisI GCNGC 6 cut(s) 315, 396, 926, 929, 1209, 1212
BlsI GCNGC 6 cut(s) 316, 397, 927, 930, 1210, 1213
Bme1390I CCNGG 5 cut(s) 101, 495, 561, 570, 622
Bme18I GGWCC 2 cut(s) 272, 1127
BmeRI GACNNNNNGTC 1 cut(s) 119
BmgT120I GGNCC 3 cut(s) 272, 480, 1127
BmiI GGNNCC 2 cut(s) 481, 510
BmrFI CCNGG 5 cut(s) 101, 495, 561, 570, 622
BmsI GCATC 4 cut(s) 321, 838, 1071, 1223
Bpu14I TTCGAA 1 cut(s) 688
BpuEI CTTGAG 2 cut(s) 365, 811
BpuMI CCSGG 1 cut(s) 101
BsaJI CCNNGG 5 cut(s) 168, 307, 493, 494, 798
BsaXI ACNNNNNCTCC 4 cut(s) 328, 358, 980, 1010
Bsc4I CCNNNNNNNGG 4 cut(s) 116, 476, 495, 1185
Bse1I ACTGG 5 cut(s) 343, 483, 510, 898, 915
BseBI CCWGG 4 cut(s) 495, 561, 570, 622
BseDI CCNNGG 5 cut(s) 168, 307, 493, 494, 798
BseGI GGATG 3 cut(s) 190, 336, 544
BseLI CCNNNNNNNGG 4 cut(s) 116, 476, 495, 1185
BseMII CTCAG 2 cut(s) 253, 573
BseNI ACTGG 5 cut(s) 343, 483, 510, 898, 915
BseSI GKGCMC 1 cut(s) 490
BseXI GCAGC 6 cut(s) 301, 382, 915, 937, 1195, 1223
BseYI CCCAGC 1 cut(s) 1184
BsgI GTGCAG 1 cut(s) 789
BshFI GGCC 5 cut(s) 144, 462, 482, 1039, 1178
BsiHKAI GWGCWC 2 cut(s) 381, 1016
BsiSI CCGG 1 cut(s) 101
BslFI GGGAC 1 cut(s) 617
BslI CCNNNNNNNGG 4 cut(s) 116, 476, 495, 1185
BsmFI GGGAC 1 cut(s) 617
BsnI GGCC 5 cut(s) 144, 462, 482, 1039, 1178
Bsp119I TTCGAA 1 cut(s) 688
Bsp1286I GDGCHC 3 cut(s) 381, 490, 1016
Bsp143I GATC 2 cut(s) 772, 1219
Bsp19I CCATGG 2 cut(s) 307, 798
BspACI CCGC 2 cut(s) 270, 512
BspANI GGCC 5 cut(s) 144, 462, 482, 1039, 1178
BspCNI CTCAG 2 cut(s) 252, 574
BspLI GGNNCC 2 cut(s) 481, 510
BspMAI CTGCAG 2 cut(s) 319, 1213
BspPI GGATC 1 cut(s) 767
BspQI GCTCTTC 1 cut(s) 1004
BspT104I TTCGAA 1 cut(s) 688
BsrI ACTGG 5 cut(s) 343, 483, 510, 898, 915
BssECI CCNNGG 5 cut(s) 168, 307, 493, 494, 798
BssMI GATC 2 cut(s) 772, 1219
BssSI CACGAG 1 cut(s) 1158
BssT1I CCWWGG 3 cut(s) 168, 307, 798
Bst2BI CACGAG 1 cut(s) 1158
Bst2UI CCWGG 4 cut(s) 495, 561, 570, 622
Bst4CI ACNGT 2 cut(s) 557, 1124
Bst6I CTCTTC 3 cut(s) 528, 817, 1004
BstAPI GCANNNNNTGC 1 cut(s) 776
BstBI TTCGAA 1 cut(s) 688
BstC8I GCNNGC 4 cut(s) 6, 393, 436, 501
BstDEI CTNAG 3 cut(s) 239, 582, 1233
BstDSI CCRYGG 2 cut(s) 307, 798
BstENI CCTNNNNNAGG 1 cut(s) 114
BstF5I GGATG 3 cut(s) 190, 336, 544
BstKTI GATC 2 cut(s) 775, 1222
BstMBI GATC 2 cut(s) 772, 1219
BstMWI GCNNNNNNNGC 4 cut(s) 776, 1045, 1175, 1190
BstNI CCWGG 4 cut(s) 495, 561, 570, 622
BstNSI RCATGY 2 cut(s) 395, 503
BstSCI CCNGG 5 cut(s) 99, 493, 559, 568, 620
BstSFI CTRYAG 3 cut(s) 315, 964, 1209
BstSLI GKGCMC 1 cut(s) 490
BstV1I GCAGC 6 cut(s) 301, 382, 915, 937, 1195, 1223
BstX2I RGATCY 1 cut(s) 772
BstYI RGATCY 1 cut(s) 772
BsuRI GGCC 5 cut(s) 144, 462, 482, 1039, 1178
BtgI CCRYGG 2 cut(s) 307, 798
BtsCI GGATG 3 cut(s) 190, 336, 544
BtsIMutI CAGTG 4 cut(s) 247, 300, 490, 503
Cac8I GCNNGC 4 cut(s) 6, 393, 436, 501
CaiI CAGNNNCTG 2 cut(s) 776, 1217
Cfr13I GGNCC 3 cut(s) 272, 480, 1127
CsiI ACCWGGT 1 cut(s) 568
Csp6I GTAC 2 cut(s) 234, 342
CviQI GTAC 2 cut(s) 234, 342
DdeI CTNAG 3 cut(s) 239, 582, 1233
DpnI GATC 2 cut(s) 774, 1221
DpnII GATC 2 cut(s) 772, 1219
DriI GACNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 1176
Eam1104I CTCTTC 3 cut(s) 528, 817, 1004
Eam1105I GACNNNNNGTC 1 cut(s) 119
EarI CTCTTC 3 cut(s) 528, 817, 1004
EciI GGCGGA 1 cut(s) 501
Ecl136II GAGCTC 1 cut(s) 1014
Eco130I CCWWGG 3 cut(s) 168, 307, 798
Eco147I AGGCCT 2 cut(s) 462, 1039
Eco24I GRGCYC 1 cut(s) 1016
Eco32I GATATC 2 cut(s) 592, 808
Eco47I GGWCC 2 cut(s) 272, 1127
Eco53kI GAGCTC 1 cut(s) 1014
Eco57I CTGAAG 1 cut(s) 56
EcoICRI GAGCTC 1 cut(s) 1014
EcoNI CCTNNNNNAGG 1 cut(s) 114
EcoRII CCWGG 4 cut(s) 493, 559, 568, 620
EcoRV GATATC 2 cut(s) 592, 808
EcoT14I CCWWGG 3 cut(s) 168, 307, 798
EcoT22I ATGCAT 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 1016
ErhI CCWWGG 3 cut(s) 168, 307, 798
FalI AAGNNNNNCTT 4 cut(s) 450, 482, 1011, 1043
FaqI GGGAC 1 cut(s) 617
FbaI TGATCA 1 cut(s) 1219
Fnu4HI GCNGC 6 cut(s) 315, 396, 926, 929, 1209, 1212
FokI GGATG 3 cut(s) 197, 343, 551
FriOI GRGCYC 1 cut(s) 1016
Fsp4HI GCNGC 6 cut(s) 315, 396, 926, 929, 1209, 1212
FspBI CTAG 4 cut(s) 111, 444, 1055, 1249
GluI GCNGC 6 cut(s) 315, 396, 926, 929, 1209, 1212
GsaI CCCAGC 1 cut(s) 1188
HaeIII GGCC 5 cut(s) 144, 462, 482, 1039, 1178
HapII CCGG 1 cut(s) 101
HincII GTYRAC 2 cut(s) 566, 838
HindII GTYRAC 2 cut(s) 566, 838
HindIII AAGCTT 1 cut(s) 432
HinfI GANTC 3 cut(s) 440, 704, 733
HpaI GTTAAC 1 cut(s) 838
HpaII CCGG 1 cut(s) 101
HphI GGTGA 1 cut(s) 807
Hpy166II GTNNAC 5 cut(s) 47, 566, 838, 961, 1127
Hpy188I TCNGA 2 cut(s) 1008, 1219
Hpy188III TCNNGA 2 cut(s) 382, 1078
Hpy8I GTNNAC 5 cut(s) 47, 566, 838, 961, 1127
HpyAV CCTTC 6 cut(s) 250, 357, 452, 476, 869, 1104
HpyCH4III ACNGT 2 cut(s) 557, 1124
HpyCH4IV ACGT 2 cut(s) 189, 844
HpyF10VI GCNNNNNNNGC 4 cut(s) 776, 1045, 1175, 1190
HpyF3I CTNAG 3 cut(s) 239, 582, 1233
HpySE526I ACGT 2 cut(s) 189, 844
Ksp22I TGATCA 1 cut(s) 1219
KspAI GTTAAC 1 cut(s) 838
Kzo9I GATC 2 cut(s) 772, 1219
LguI GCTCTTC 1 cut(s) 1004
LmnI GCTCC 3 cut(s) 514, 892, 988
Lsp1109I GCAGC 6 cut(s) 301, 382, 915, 937, 1195, 1223
LweI GCATC 4 cut(s) 321, 838, 1071, 1223
MabI ACCWGGT 1 cut(s) 568
MaeI CTAG 4 cut(s) 111, 444, 1055, 1249
MaeII ACGT 2 cut(s) 189, 844
MaeIII GTNAC 3 cut(s) 404, 635, 734
MalI GATC 2 cut(s) 774, 1221
MboI GATC 2 cut(s) 772, 1219
MboII GAAGA 9 cut(s) 49, 397, 545, 702, 705, 804, 815, 857, 1021
MflI RGATCY 1 cut(s) 772
MhlI GDGCHC 3 cut(s) 381, 490, 1016
MlsI TGGCCA 1 cut(s) 1178
MluCI AATT 2 cut(s) 939, 1138
MluNI TGGCCA 1 cut(s) 1178
MlyI GAGTC 2 cut(s) 449, 742
Mox20I TGGCCA 1 cut(s) 1178
Mph1103I ATGCAT 1 cut(s) 25
MroXI GAANNNNTTC 1 cut(s) 684
MscI TGGCCA 1 cut(s) 1178
MseI TTAA 4 cut(s) 192, 755, 837, 1029
MslI CAYNNNNRTG 2 cut(s) 374, 797
Msp20I TGGCCA 1 cut(s) 1178
MspA1I CMGCKG 1 cut(s) 928
MspI CCGG 1 cut(s) 101
MspR9I CCNGG 5 cut(s) 101, 495, 561, 570, 622
MvaI CCWGG 4 cut(s) 495, 561, 570, 622
MwoI GCNNNNNNNGC 4 cut(s) 776, 1045, 1175, 1190
NciI CCSGG 1 cut(s) 101
NcoI CCATGG 2 cut(s) 307, 798
NdeII GATC 2 cut(s) 772, 1219
NlaIV GGNNCC 2 cut(s) 481, 510
NmuCI GTSAC 2 cut(s) 635, 734
NsiI ATGCAT 1 cut(s) 25
NspI RCATGY 2 cut(s) 395, 503
NspV TTCGAA 1 cut(s) 688
PaeI GCATGC 2 cut(s) 395, 503
PasI CCCWGGG 1 cut(s) 494
PceI AGGCCT 2 cut(s) 462, 1039
PciSI GCTCTTC 1 cut(s) 1004
PdmI GAANNNNTTC 1 cut(s) 684
PfeI GAWTC 1 cut(s) 704
PflMI CCANNNNNTGG 1 cut(s) 476
PfoI TCCNGGA 1 cut(s) 620
PkrI GCNGC 6 cut(s) 316, 397, 927, 930, 1210, 1213
PleI GAGTC 2 cut(s) 448, 741
PpsI GAGTC 2 cut(s) 448, 741
PsiI TTATAA 1 cut(s) 156
Psp124BI GAGCTC 1 cut(s) 1016
Psp6I CCWGG 4 cut(s) 493, 559, 568, 620
PspFI CCCAGC 1 cut(s) 1184
PspGI CCWGG 4 cut(s) 493, 559, 568, 620
PspN4I GGNNCC 2 cut(s) 481, 510
PspPI GGNCC 3 cut(s) 272, 480, 1127
PstI CTGCAG 2 cut(s) 319, 1213
PstNI CAGNNNCTG 2 cut(s) 776, 1217
PsuI RGATCY 1 cut(s) 772
PvuII CAGCTG 1 cut(s) 928
RsaI GTAC 2 cut(s) 235, 343
RsaNI GTAC 2 cut(s) 234, 342
RseI CAYNNNNRTG 2 cut(s) 374, 797
SacI GAGCTC 1 cut(s) 1016
SapI GCTCTTC 1 cut(s) 1004
SaqAI TTAA 4 cut(s) 192, 755, 837, 1029
SatI GCNGC 6 cut(s) 315, 396, 926, 929, 1209, 1212
Sau3AI GATC 2 cut(s) 772, 1219
Sau96I GGNCC 3 cut(s) 272, 480, 1127
SchI GAGTC 2 cut(s) 449, 742
ScrFI CCNGG 5 cut(s) 101, 495, 561, 570, 622
SduI GDGCHC 3 cut(s) 381, 490, 1016
SexAI ACCWGGT 1 cut(s) 568
SfaNI GCATC 4 cut(s) 321, 838, 1071, 1223
SfcI CTRYAG 3 cut(s) 315, 964, 1209
SfuI TTCGAA 1 cut(s) 688
SinI GGWCC 2 cut(s) 272, 1127
SmiMI CAYNNNNRTG 2 cut(s) 374, 797
SmlI CTYRAG 2 cut(s) 380, 826
SmoI CTYRAG 2 cut(s) 380, 826
SphI GCATGC 2 cut(s) 395, 503
Sse9I AATT 2 cut(s) 939, 1138
SseBI AGGCCT 2 cut(s) 462, 1039
SsiI CCGC 2 cut(s) 270, 512
SspMI CTAG 4 cut(s) 111, 444, 1055, 1249
SstI GAGCTC 1 cut(s) 1016
StuI AGGCCT 2 cut(s) 462, 1039
StyD4I CCNGG 5 cut(s) 99, 493, 559, 568, 620
StyI CCWWGG 3 cut(s) 168, 307, 798
TaaI ACNGT 2 cut(s) 557, 1124
TaiI ACGT 2 cut(s) 192, 847
TaqI TCGA 2 cut(s) 688, 933
TasI AATT 2 cut(s) 939, 1138
TfiI GAWTC 1 cut(s) 704
Tru1I TTAA 4 cut(s) 192, 755, 837, 1029
Tru9I TTAA 4 cut(s) 192, 755, 837, 1029
TscAI CASTG 4 cut(s) 247, 307, 490, 510
TseFI GTSAC 2 cut(s) 635, 734
TseI GCWGC 6 cut(s) 314, 395, 925, 928, 1208, 1211
Tsp45I GTSAC 2 cut(s) 635, 734
TspDTI ATGAA 7 cut(s) 64, 280, 693, 851, 857, 951, 1163
TspRI CASTG 4 cut(s) 247, 307, 490, 510
Van91I CCANNNNNTGG 1 cut(s) 476
VpaK11BI GGWCC 2 cut(s) 272, 1127
XagI CCTNNNNNAGG 1 cut(s) 114
XceI RCATGY 2 cut(s) 395, 503
XcmI CCANNNNNNNNNTGG 1 cut(s) 1201
XmnI GAANNNNTTC 1 cut(s) 684
XspI CTAG 4 cut(s) 111, 444, 1055, 1249
Zsp2I ATGCAT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.