MD09G1167100.v1.1

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001085
Physical Location & Seq
Forward (+)
13705947 .. 13708533
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1167100.v1.1.491

Sequence Viewer

Length: 1464 bp
ATGTTAGACTATGTGACCTTAATCTGCAGAATGGCTTGTGGAGCATTACTATTTGTTGCTTTGTTAAGTTTGATTGTTGGGAGCTGGTGCCTCCCAGAAAATGCAGAACTTAAAGATTTCGGTACTAATAAGGATAATGGCCCTCCAAGTAACACTGAGAAAAAGGGAAATAGATGGGCAGTTCTAGTTGCTGGGTCAAATGAATACTACAACTATAGACACCAGGCTGATATATCCCATGCATACCAGATACTGAAGAAAGGCGGACTGAAAGATGAGAACATCATTGTTTTTATGTACGATGATATTGCATACAACCCGGAAAATCCTAGACAAGGTGTCATCGTCAACAAACCAAATGGCCCTAATGTTTATAAAGGAGTTCCCAAGGATTATACAGGAACTGATGTTAATTCAAACAATTTCTATAATGTTATTCTTGGAAACAAAAGTGCTCTCACTGGAGGGAGTGGCAAGGTTCTGCTTAGCGGTCCAAATGACCATGTTTTCATATATTATGCAGACCATGGTTCTGCAGGATTACTTGGTACAATGAAGGAAGAAATTTTTAAAATTTTAGTACTTTACGTTGAAGCTTGCGAGTCTGGGAGCATGTTTGATGGTATCCTTCCGAGAAATCTAAATATTTATGCTACTACCTCGGCAAATCCAGAAGAGAGCAGTTATGGAACATATTGTCCTGGTGAAGATCCTCCTGTTGCTCAAGAGTATGGTACTTGTTTGGGAGACTTGTATAGTATTTCCTGGATGGAGGATTGTGACATAAGTGATTCGCGCAAAGAAACTTTGCAGCAGCAGTATGAAAGGGTAACATGCATATTGAATTTTATATCGTTATGCGTAATAATATTAGCAAACATAAGAATTGTATATATGACCATGTCGGCTAAATTACAGGTTCGAAGAAGAACAAATAAATCTCATGTTATGCAGTATGGAGATATGAGTCGCAGACAGCAGTTCCTTGTCACTTACATGGGTGGATATCTTTCTAAACATAGTACTTCCACCAGCGATATCTCTTCACCCTTAATCTCAAGGGTTGTTAACCAACGCGACACTAAGCTCCACTATTTTCAGCACAAGTTGCGTAGAGCTCCGACTGGCTCTCAGGAGGAATCGGAAGCTCGAAAGCAGCTACTCGATGAAATTGCTCATCGGAAACATGTGGACGATAGCATACATAAAATAGGGGAGCTTTTGTTTGGACACCGGGAGAGCTCAAACATGTTGATGAAGGGTAGGCCTCGAGGGCAACCTCTAGTAGATAACTGGGACTGCTTCAAGAAGCTTTTGAAGATTTACGAGAAGTATTGTGGACATTTTTCTGCATACGGAATGAAACACACGCGAGCTATAGCCAACATGTGCAACGCTGGGATCACAGCAGAGAAAATGGTTGTGGCGTCTGATCAGACTTGTTCCAAAAAACCCCATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

488

Amino Acids

54.72

Weight (kDa)

8.27

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 58 - 185 2.7e-59 Peptidase C13 family
Peptidase_C13 PF01650 187 - 277 1.2e-30 Peptidase C13 family
Legum_prodom PF20985 385 - 481 6.9e-38 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
AbsI CCTCGAGG 1 cut(s) 1269
AccB1I GGYRCC 1 cut(s) 87
AccII CGCG 3 cut(s) 796, 1077, 1372
AciI CCGC 2 cut(s) 264, 489
AclWI GGATC 2 cut(s) 704, 1409
AcsI RAATTY 3 cut(s) 564, 573, 844
AcuI CTGAAG 1 cut(s) 275
AcyI GRCGYC 1 cut(s) 1427
AfaI GTAC 6 cut(s) 124, 299, 550, 582, 736, 1024
AfiI CCNNNNNNNGG 1 cut(s) 335
AflIII ACRYGT 3 cut(s) 1186, 1248, 1386
AgsI TTSAA 5 cut(s) 417, 593, 844, 1306, 1318
AhdI GACNNNNNGTC 1 cut(s) 338
AjnI CCWGG 3 cut(s) 222, 700, 764
AloI GAACNNNNNNTCC 2 cut(s) 682, 714
Alw21I GWGCWC 3 cut(s) 457, 1120, 1244
Alw26I GTCTC 1 cut(s) 741
AlwI GGATC 2 cut(s) 704, 1409
AlwNI CAGNNNCTG 2 cut(s) 253, 404
Ama87I CYCGRG 1 cut(s) 1269
AoxI GGCC 3 cut(s) 139, 361, 1265
ApeKI GCWGC 3 cut(s) 811, 814, 1156
ApoI RAATTY 3 cut(s) 564, 573, 844
AspLEI GCGC 1 cut(s) 798
AspS9I GGNCC 3 cut(s) 140, 362, 491
AsuC2I CCSGG 2 cut(s) 320, 1235
AsuHPI GGTGA 2 cut(s) 716, 1038
AsuII TTCGAA 1 cut(s) 922
AvaI CYCGRG 1 cut(s) 1269
AvaII GGWCC 1 cut(s) 491
BanI GGYRCC 1 cut(s) 87
BanII GRGCYC 2 cut(s) 1120, 1244
Bbv12I GWGCWC 3 cut(s) 457, 1120, 1244
BbvI GCAGC 3 cut(s) 823, 826, 1168
BccI CCATC 3 cut(s) 168, 614, 763
BcgI CGANNNNNNTGC 4 cut(s) 290, 324, 1154, 1188
BciT130I CCWGG 3 cut(s) 224, 702, 766
BciVI GTATCC 1 cut(s) 635
BclI TGATCA 1 cut(s) 1432
BcnI CCSGG 2 cut(s) 320, 1235
BcoDI GTCTC 1 cut(s) 741
BfaI CTAG 3 cut(s) 185, 330, 1283
BfmI CTRYAG 4 cut(s) 25, 214, 534, 1377
BfuI GTATCC 1 cut(s) 635
BglI GCCNNNNNGGC 1 cut(s) 1273
BisI GCNGC 3 cut(s) 812, 815, 1157
BlpI GCTNAGC 1 cut(s) 485
BlsI GCNGC 3 cut(s) 813, 816, 1158
BmcAI AGTACT 2 cut(s) 582, 1024
Bme1390I CCNGG 5 cut(s) 224, 320, 702, 766, 1235
Bme18I GGWCC 1 cut(s) 491
BmeRI GACNNNNNGTC 1 cut(s) 338
BmeT110I CYCGRG 1 cut(s) 1269
BmgT120I GGNCC 3 cut(s) 140, 362, 491
BmiI GGNNCC 1 cut(s) 89
BmrFI CCNGG 5 cut(s) 224, 320, 702, 766, 1235
BmrI ACTGGG 1 cut(s) 1303
BmuI ACTGGG 1 cut(s) 1303
BpmI CTGGAG 1 cut(s) 483
Bpu1102I GCTNAGC 1 cut(s) 485
Bpu14I TTCGAA 1 cut(s) 922
BpuEI CTTGAG 2 cut(s) 708, 1042
BpuMI CCSGG 2 cut(s) 320, 1235
BsaHI GRCGYC 1 cut(s) 1427
BsaJI CCNNGG 3 cut(s) 387, 526, 660
BsaXI ACNNNNNCTCC 2 cut(s) 1208, 1238
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse1I ACTGG 3 cut(s) 466, 1129, 1298
BseBI CCWGG 3 cut(s) 224, 702, 766
BseDI CCNNGG 3 cut(s) 387, 526, 660
BseGI GGATG 1 cut(s) 774
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMII CTCAG 2 cut(s) 147, 1145
BseNI ACTGG 3 cut(s) 466, 1129, 1298
BseXI GCAGC 3 cut(s) 823, 826, 1168
BseYI CCCAGC 2 cut(s) 191, 1397
Bsh1236I CGCG 3 cut(s) 796, 1077, 1372
BshFI GGCC 3 cut(s) 141, 363, 1267
BshNI GGYRCC 1 cut(s) 87
BsiHKAI GWGCWC 3 cut(s) 457, 1120, 1244
BsiHKCI CYCGRG 1 cut(s) 1269
BsiSI CCGG 2 cut(s) 320, 1234
BslFI GGGAC 1 cut(s) 1310
BslI CCNNNNNNNGG 1 cut(s) 335
BsmAI GTCTC 1 cut(s) 741
BsmFI GGGAC 1 cut(s) 1310
BsnI GGCC 3 cut(s) 141, 363, 1267
BsoBI CYCGRG 1 cut(s) 1269
Bsp119I TTCGAA 1 cut(s) 922
Bsp1286I GDGCHC 3 cut(s) 457, 1120, 1244
Bsp143I GATC 3 cut(s) 709, 1401, 1432
Bsp1720I GCTNAGC 1 cut(s) 485
Bsp19I CCATGG 1 cut(s) 526
BspACI CCGC 2 cut(s) 264, 489
BspANI GGCC 3 cut(s) 141, 363, 1267
BspCNI CTCAG 2 cut(s) 148, 1144
BspFNI CGCG 3 cut(s) 796, 1077, 1372
BspLI GGNNCC 1 cut(s) 89
BspMAI CTGCAG 2 cut(s) 29, 538
BspPI GGATC 2 cut(s) 704, 1409
BspT104I TTCGAA 1 cut(s) 922
BspT107I GGYRCC 1 cut(s) 87
BsrI ACTGG 3 cut(s) 466, 1129, 1298
BssECI CCNNGG 3 cut(s) 387, 526, 660
BssMI GATC 3 cut(s) 709, 1401, 1432
BssNI GRCGYC 1 cut(s) 1427
BssT1I CCWWGG 2 cut(s) 387, 526
Bst2UI CCWGG 3 cut(s) 224, 702, 766
Bst6I CTCTTC 2 cut(s) 669, 1048
BstACI GRCGYC 1 cut(s) 1427
BstAPI GCANNNNNTGC 1 cut(s) 1108
BstBI TTCGAA 1 cut(s) 922
BstC8I GCNNGC 2 cut(s) 598, 1374
BstDEI CTNAG 4 cut(s) 156, 485, 1083, 1131
BstDSI CCRYGG 1 cut(s) 526
BstENI CCTNNNNNAGG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 774
BstFNI CGCG 3 cut(s) 796, 1077, 1372
BstHHI GCGC 1 cut(s) 798
BstKTI GATC 3 cut(s) 712, 1404, 1435
BstMAI GTCTC 1 cut(s) 741
BstMBI GATC 3 cut(s) 709, 1401, 1432
BstMWI GCNNNNNNNGC 3 cut(s) 41, 1108, 1273
BstNI CCWGG 3 cut(s) 224, 702, 766
BstNSI RCATGY 5 cut(s) 616, 837, 1190, 1252, 1390
BstSCI CCNGG 5 cut(s) 222, 318, 700, 764, 1233
BstSFI CTRYAG 4 cut(s) 25, 214, 534, 1377
BstUI CGCG 3 cut(s) 796, 1077, 1372
BstV1I GCAGC 3 cut(s) 823, 826, 1168
BstX2I RGATCY 1 cut(s) 709
BstYI RGATCY 1 cut(s) 709
BsuI GTATCC 1 cut(s) 635
BsuRI GGCC 3 cut(s) 141, 363, 1267
BtgI CCRYGG 1 cut(s) 526
BtsCI GGATG 1 cut(s) 774
BtsIMutI CAGTG 2 cut(s) 153, 459
Cac8I GCNNGC 2 cut(s) 598, 1374
CaiI CAGNNNCTG 2 cut(s) 253, 404
CfoI GCGC 1 cut(s) 798
Cfr13I GGNCC 3 cut(s) 140, 362, 491
CseI GACGC 1 cut(s) 1416
Csp6I GTAC 6 cut(s) 123, 298, 549, 581, 735, 1023
CviQI GTAC 6 cut(s) 123, 298, 549, 581, 735, 1023
DdeI CTNAG 4 cut(s) 156, 485, 1083, 1131
DpnI GATC 3 cut(s) 711, 1403, 1434
DpnII GATC 3 cut(s) 709, 1401, 1432
DraI TTTAAA 1 cut(s) 571
DriI GACNNNNNGTC 1 cut(s) 338
Eam1104I CTCTTC 2 cut(s) 669, 1048
Eam1105I GACNNNNNGTC 1 cut(s) 338
EarI CTCTTC 2 cut(s) 669, 1048
EciI GGCGGA 1 cut(s) 279
Ecl136II GAGCTC 2 cut(s) 1118, 1242
Eco130I CCWWGG 2 cut(s) 387, 526
Eco147I AGGCCT 1 cut(s) 1267
Eco24I GRGCYC 2 cut(s) 1120, 1244
Eco32I GATATC 2 cut(s) 1007, 1039
Eco47I GGWCC 1 cut(s) 491
Eco53kI GAGCTC 2 cut(s) 1118, 1242
Eco57I CTGAAG 1 cut(s) 275
Eco88I CYCGRG 1 cut(s) 1269
EcoICRI GAGCTC 2 cut(s) 1118, 1242
EcoNI CCTNNNNNAGG 1 cut(s) 333
EcoRII CCWGG 3 cut(s) 222, 700, 764
EcoRV GATATC 2 cut(s) 1007, 1039
EcoT14I CCWWGG 2 cut(s) 387, 526
EcoT22I ATGCAT 2 cut(s) 244, 839
EcoT38I GRGCYC 2 cut(s) 1120, 1244
ErhI CCWWGG 2 cut(s) 387, 526
FaqI GGGAC 1 cut(s) 1310
FbaI TGATCA 1 cut(s) 1432
Fnu4HI GCNGC 3 cut(s) 812, 815, 1157
FokI GGATG 1 cut(s) 781
FriOI GRGCYC 2 cut(s) 1120, 1244
Fsp4HI GCNGC 3 cut(s) 812, 815, 1157
FspBI CTAG 3 cut(s) 185, 330, 1283
GlaI GCGC 1 cut(s) 797
GluI GCNGC 3 cut(s) 812, 815, 1157
GsaI CCCAGC 2 cut(s) 195, 1401
GsuI CTGGAG 1 cut(s) 483
HaeIII GGCC 3 cut(s) 141, 363, 1267
HapII CCGG 2 cut(s) 320, 1234
HgaI GACGC 1 cut(s) 1416
HhaI GCGC 1 cut(s) 798
Hin1I GRCGYC 1 cut(s) 1427
Hin6I GCGC 1 cut(s) 796
HinP1I GCGC 1 cut(s) 796
HincII GTYRAC 2 cut(s) 349, 1069
HindII GTYRAC 2 cut(s) 349, 1069
HindIII AAGCTT 2 cut(s) 594, 1310
HinfI GANTC 4 cut(s) 602, 791, 967, 1139
HpaI GTTAAC 1 cut(s) 1069
HpaII CCGG 2 cut(s) 320, 1234
HphI GGTGA 2 cut(s) 716, 1038
Hpy166II GTNNAC 4 cut(s) 349, 1069, 1192, 1340
Hpy188I TCNGA 6 cut(s) 633, 1122, 1144, 1182, 1432, 1437
Hpy188III TCNNGA 4 cut(s) 671, 725, 1133, 1306
Hpy8I GTNNAC 4 cut(s) 349, 1069, 1192, 1340
HpyAV CCTTC 3 cut(s) 550, 638, 1252
HpyCH4IV ACGT 1 cut(s) 588
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 1108, 1273
HpyF3I CTNAG 4 cut(s) 156, 485, 1083, 1131
HpySE526I ACGT 1 cut(s) 588
Hsp92I GRCGYC 1 cut(s) 1427
HspAI GCGC 1 cut(s) 796
Ksp22I TGATCA 1 cut(s) 1432
KspAI GTTAAC 1 cut(s) 1069
Kzo9I GATC 3 cut(s) 709, 1401, 1432
LmnI GCTCC 6 cut(s) 41, 81, 609, 1092, 1123, 1216
Lsp1109I GCAGC 3 cut(s) 823, 826, 1168
MaeI CTAG 3 cut(s) 185, 330, 1283
MaeII ACGT 1 cut(s) 588
MaeIII GTNAC 5 cut(s) 13, 149, 779, 829, 988
MalI GATC 3 cut(s) 711, 1403, 1434
MboI GATC 3 cut(s) 709, 1401, 1432
MboII GAAGA 8 cut(s) 268, 572, 686, 719, 936, 939, 1035, 1330
MflI RGATCY 1 cut(s) 709
MhlI GDGCHC 3 cut(s) 457, 1120, 1244
MluCI AATT 8 cut(s) 412, 421, 564, 573, 844, 885, 911, 1170
MlyI GAGTC 2 cut(s) 611, 976
MmeI TCCRAC 1 cut(s) 1145
Mph1103I ATGCAT 2 cut(s) 244, 839
MseI TTAA 7 cut(s) 20, 65, 111, 411, 570, 1052, 1068
MslI CAYNNNNRTG 2 cut(s) 995, 1253
MspI CCGG 2 cut(s) 320, 1234
MspR9I CCNGG 5 cut(s) 224, 320, 702, 766, 1235
MvaI CCWGG 3 cut(s) 224, 702, 766
MvnI CGCG 3 cut(s) 796, 1077, 1372
MwoI GCNNNNNNNGC 3 cut(s) 41, 1108, 1273
NciI CCSGG 2 cut(s) 320, 1235
NcoI CCATGG 1 cut(s) 526
NdeII GATC 3 cut(s) 709, 1401, 1432
NlaIV GGNNCC 1 cut(s) 89
NmeAIII GCCGAG 1 cut(s) 641
NmuCI GTSAC 3 cut(s) 13, 779, 988
NsiI ATGCAT 2 cut(s) 244, 839
NspI RCATGY 5 cut(s) 616, 837, 1190, 1252, 1390
NspV TTCGAA 1 cut(s) 922
PaeR7I CTCGAG 1 cut(s) 1269
PceI AGGCCT 1 cut(s) 1267
PciI ACATGT 3 cut(s) 1186, 1248, 1386
PfeI GAWTC 2 cut(s) 791, 1139
PflFI GACNNNGTC 1 cut(s) 901
PfoI TCCNGGA 1 cut(s) 764
PkrI GCNGC 3 cut(s) 813, 816, 1158
PleI GAGTC 2 cut(s) 610, 975
PpsI GAGTC 2 cut(s) 610, 975
PscI ACATGT 3 cut(s) 1186, 1248, 1386
PsiI TTATAA 1 cut(s) 375
Psp124BI GAGCTC 2 cut(s) 1120, 1244
Psp6I CCWGG 3 cut(s) 222, 700, 764
PspFI CCCAGC 2 cut(s) 191, 1397
PspGI CCWGG 3 cut(s) 222, 700, 764
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 3 cut(s) 140, 362, 491
PspXI VCTCGAGB 1 cut(s) 1269
PstI CTGCAG 2 cut(s) 29, 538
PstNI CAGNNNCTG 2 cut(s) 253, 404
PsuI RGATCY 1 cut(s) 709
PsyI GACNNNGTC 1 cut(s) 901
RsaI GTAC 6 cut(s) 124, 299, 550, 582, 736, 1024
RsaNI GTAC 6 cut(s) 123, 298, 549, 581, 735, 1023
RseI CAYNNNNRTG 2 cut(s) 995, 1253
SacI GAGCTC 2 cut(s) 1120, 1244
SaqAI TTAA 7 cut(s) 20, 65, 111, 411, 570, 1052, 1068
SatI GCNGC 3 cut(s) 812, 815, 1157
Sau3AI GATC 3 cut(s) 709, 1401, 1432
Sau96I GGNCC 3 cut(s) 140, 362, 491
ScaI AGTACT 2 cut(s) 582, 1024
SchI GAGTC 2 cut(s) 611, 976
ScrFI CCNGG 5 cut(s) 224, 320, 702, 766, 1235
SduI GDGCHC 3 cut(s) 457, 1120, 1244
SfcI CTRYAG 4 cut(s) 25, 214, 534, 1377
Sfr274I CTCGAG 1 cut(s) 1269
SfuI TTCGAA 1 cut(s) 922
SinI GGWCC 1 cut(s) 491
SlaI CTCGAG 1 cut(s) 1269
SmiMI CAYNNNNRTG 2 cut(s) 995, 1253
SmlI CTYRAG 3 cut(s) 723, 1057, 1269
SmoI CTYRAG 3 cut(s) 723, 1057, 1269
Sse9I AATT 8 cut(s) 412, 421, 564, 573, 844, 885, 911, 1170
SseBI AGGCCT 1 cut(s) 1267
SsiI CCGC 2 cut(s) 264, 489
SspI AATATT 2 cut(s) 646, 870
SspMI CTAG 3 cut(s) 185, 330, 1283
SstI GAGCTC 2 cut(s) 1120, 1244
StuI AGGCCT 1 cut(s) 1267
StyD4I CCNGG 5 cut(s) 222, 318, 700, 764, 1233
StyI CCWWGG 2 cut(s) 387, 526
TaiI ACGT 1 cut(s) 591
TaqI TCGA 4 cut(s) 922, 1150, 1164, 1270
TasI AATT 8 cut(s) 412, 421, 564, 573, 844, 885, 911, 1170
TatI WGTACW 2 cut(s) 580, 1022
TfiI GAWTC 2 cut(s) 791, 1139
Tru1I TTAA 7 cut(s) 20, 65, 111, 411, 570, 1052, 1068
Tru9I TTAA 7 cut(s) 20, 65, 111, 411, 570, 1052, 1068
TscAI CASTG 2 cut(s) 160, 466
TseFI GTSAC 3 cut(s) 13, 779, 988
TseI GCWGC 3 cut(s) 811, 814, 1156
Tsp45I GTSAC 3 cut(s) 13, 779, 988
TspDTI ATGAA 7 cut(s) 216, 499, 569, 837, 1182, 1271, 1376
TspGWI ACGGA 1 cut(s) 1371
TspRI CASTG 2 cut(s) 160, 466
Tth111I GACNNNGTC 1 cut(s) 901
VpaK11BI GGWCC 1 cut(s) 491
XagI CCTNNNNNAGG 1 cut(s) 333
XapI RAATTY 3 cut(s) 564, 573, 844
XceI RCATGY 5 cut(s) 616, 837, 1190, 1252, 1390
XhoI CTCGAG 1 cut(s) 1269
XspI CTAG 3 cut(s) 185, 330, 1283
ZrmI AGTACT 2 cut(s) 582, 1024
Zsp2I ATGCAT 2 cut(s) 244, 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.