RchiOBHm_Chr2g0149101

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001085
Physical Location & Seq
Reverse (-)
66924220 .. 66927997
3778 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51852

Sequence Viewer

Length: 1752 bp
ATGAGTATTCAAGGTTTAGTAGTACTTCTTTTTCTGTCTGTTTTGAGTGTGAGTACTATTGGGAGCACTCTTGTTGAAAATGGTTCTACGAGTACCACTCATCATAAGTCCAAGGGAAAAAGATGGGCACTTCTAATTGCTGGTTCAAATGGTTACGATAACTATCGACACCAGGCTGATGTATGCCATGCATACCAGATACTTAAGAAAGGTGGACTCAAAGATGAAAACATTATTGTCTTCATGTATGATGATATTGCTAACAACACAGAAAACCCTAGACCTGGTGTCATTATCAACAAGCCAAACGGCAAGGATGTCTACCACGGAGTTCCCAAGGATTACACAGGAGATGATGTTAACTCACACAATTTCTACGCTGCTATTCTCGGAGAGAAAACTGCACTCAAAGGAGGAAGTGGCAAGGTTCTGGAGACTGGTCCAAATGACCATATTTTCATATATTACACTGATCATGGTGCTACAGGACTTCTTGGGATGCCTAGTGAAAGTGACTTTGTTTATGCTAAAGATCTGATACATGTGCTGAAAAAGAAGCACGCTGCTAAAGGTTACAAAAGCATGGTGTTTTACCTTGAAGCTTGTGAGTCTGGGAGTATGTTTGAGGGTTTGCTTCCAAAGAATATGAACATTTATGCTACTACAGCATCAAATGCAGAGGAGAGTAGTTATGCAACATACTGTGATGGTGATCCTGGTGTTCCTACAGAATTCGGTACTTGTTTGGGAGACTTGTATAGTATTTCCTGGTTGGAGGATTGCGATGCAAATGATCTGCGGAAAGAGACTTTTGGGGTTCAATATAAAAGGGTCCGAAGCAGAACAAATGAATCTCATGTTATGCAGTATGGAGATATGAGTCACATAAAGGAATTCCTCTACACTTACATGGGGGCAACTCCTAAAACTCATAGCTATGCTCCCTTCACATCCTCGCCTTCGGAATCAATCTCAAGGACTGTTAGCCAGCGGGATGTAGCCCTCCTATATTTTCTGCAAAAGTTAAACAGAGCTCCAACTGGATCTCATGAGAAGATCGAAGCTCAAAAGCAGCTACGTAAAGAAATTGCTCGAAGGAAACATGTGGACTACAGCATAACCAAAATAGGGGAGCTTCTGTTTGGGACCGAGAGGAGCTCAAAAGTATTGATGAATGTCAGGCCTTCGGGACAACCTCTAGTGGATGACTGGGAGTGCTTCAAGATGTTTATAAGGACTTATGAGAGTCGCTGTGGAAAGCTATCGAAATATGGAATGAAATACACGCGAGCTTTTGCCAACATGTGCAATGCCGGGGTCAGCCTAGAGAAACTTGTTGCAGTGTCTGATCAAGCTTGTTCTAAGAAACATATGAGAACATCATCGTTTTCATGTACGACGATATTGCATCAGACAGAGAAAATTCCAGGCCTGGTGTCATTATCGATCAACAATCGAAATGGTATTGATGTTTACAATGGAGTTCCCAAGCAGGATTATGCATTAGGTGATGTTAATATAGTCAATCTTTTCGCTGTTATTCTCGGAAACAAAAGTGACCTCATTGGAGAAAGTGGCAAAGTTCTCGACAGTGCTGCATTTGGAGGGAGTCTATTTGAGGGTCTCCTGCCAGATAGAATGAGTATCTATGCTACTGCAGCATCACGAGAAAGTTTTGCAGCATGTCAACATGATCCTGCTGTTCCTGCAGAATATGCCACCTATTTGGGAGACTTGTATAGTATTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

583

Amino Acids

64.37

Weight (kDa)

7.26

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 41 - 305 1.2e-116 Peptidase C13 family
Legum_prodom PF20985 357 - 453 3.2e-39 Legumain, prodomain
Peptidase_C13 PF01650 483 - 532 5.8e-06 Peptidase C13 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1232
AccI GTMKAC 1 cut(s) 321
AccII CGCG 1 cut(s) 1288
AciI CCGC 2 cut(s) 799, 991
AclWI GGATC 3 cut(s) 707, 1051, 1688
AcsI RAATTY 3 cut(s) 731, 893, 1422
AfaI GTAC 5 cut(s) 24, 55, 94, 739, 1396
AfiI CCNNNNNNNGG 2 cut(s) 284, 1128
AflII CTTAAG 1 cut(s) 203
AflIII ACRYGT 3 cut(s) 541, 1102, 1302
AgsI TTSAA 6 cut(s) 11, 77, 147, 599, 821, 1222
AhdI GACNNNNNGTC 1 cut(s) 287
AjnI CCWGG 6 cut(s) 171, 283, 715, 767, 1426, 1431
Alw21I GWGCWC 3 cut(s) 68, 1036, 1160
Alw26I GTCTC 5 cut(s) 428, 744, 800, 1628, 1725
AlwI GGATC 3 cut(s) 707, 1051, 1688
AlwNI CAGNNNCTG 1 cut(s) 1346
AoxI GGCC 2 cut(s) 1181, 1429
ApeKI GCWGC 6 cut(s) 380, 563, 1072, 1595, 1658, 1679
ApoI RAATTY 3 cut(s) 731, 893, 1422
AspS9I GGNCC 3 cut(s) 440, 832, 1146
AsuC2I CCSGG 1 cut(s) 1315
AsuHPI GGTGA 2 cut(s) 722, 1520
AvaII GGWCC 3 cut(s) 440, 832, 1146
BaeGI GKGCMC 1 cut(s) 130
BanII GRGCYC 2 cut(s) 1036, 1160
BauI CACGAG 1 cut(s) 1665
BbsI GAAGAC 1 cut(s) 232
Bbv12I GWGCWC 3 cut(s) 68, 1036, 1160
BbvI GCAGC 6 cut(s) 367, 550, 1084, 1582, 1670, 1691
BccI CCATC 2 cut(s) 117, 701
BceAI ACGGC 1 cut(s) 325
BcgI CGANNNNNNTGC 6 cut(s) 1387, 1421, 1567, 1577, 1601, 1611
BciT130I CCWGG 6 cut(s) 173, 285, 717, 769, 1428, 1433
BclI TGATCA 2 cut(s) 472, 1348
BcnI CCSGG 1 cut(s) 1315
BcoDI GTCTC 5 cut(s) 428, 744, 800, 1628, 1725
BfaI CTAG 4 cut(s) 279, 504, 1199, 1325
BfmI CTRYAG 6 cut(s) 483, 663, 726, 1111, 1656, 1707
BfrI CTTAAG 1 cut(s) 203
BglII AGATCT 1 cut(s) 532
BisI GCNGC 6 cut(s) 381, 564, 1073, 1596, 1659, 1680
BlsI GCNGC 6 cut(s) 382, 565, 1074, 1597, 1660, 1681
BmcAI AGTACT 2 cut(s) 24, 55
Bme1390I CCNGG 7 cut(s) 173, 285, 717, 769, 1315, 1428, 1433
Bme18I GGWCC 3 cut(s) 440, 832, 1146
BmeRI GACNNNNNGTC 1 cut(s) 287
BmgT120I GGNCC 3 cut(s) 440, 832, 1146
BmiI GGNNCC 2 cut(s) 833, 1147
BmrFI CCNGG 7 cut(s) 173, 285, 717, 769, 1315, 1428, 1433
BmrI ACTGGG 1 cut(s) 1219
BmsI GCATC 5 cut(s) 489, 677, 775, 1417, 1670
BmuI ACTGGG 1 cut(s) 1219
BpiI GAAGAC 1 cut(s) 232
BplI GAGNNNNNCTC 4 cut(s) 82, 114, 1142, 1174
BpmI CTGGAG 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 958
BpuMI CCSGG 1 cut(s) 1315
Bsa29I ATCGAT 1 cut(s) 1445
BsaAI YACGTR 1 cut(s) 1079
BsaBI GATNNNNATC 2 cut(s) 162, 711
BsaI GGTCTC 1 cut(s) 1628
BsaJI CCNNGG 4 cut(s) 111, 325, 336, 1314
BsaXI ACNNNNNCTCC 4 cut(s) 342, 372, 1124, 1154
Bsc4I CCNNNNNNNGG 2 cut(s) 284, 1128
Bse1I ACTGG 3 cut(s) 442, 1045, 1214
Bse3DI GCAATG 1 cut(s) 1315
Bse8I GATNNNNATC 2 cut(s) 162, 711
BseBI CCWGG 6 cut(s) 173, 285, 717, 769, 1428, 1433
BseCI ATCGAT 1 cut(s) 1445
BseDI CCNNGG 4 cut(s) 111, 325, 336, 1314
BseGI GGATG 5 cut(s) 322, 504, 950, 1000, 1210
BseJI GATNNNNATC 2 cut(s) 162, 711
BseLI CCNNNNNNNGG 2 cut(s) 284, 1128
BseMI GCAATG 1 cut(s) 1315
BseNI ACTGG 3 cut(s) 442, 1045, 1214
BseRI GAGGAG 2 cut(s) 695, 1168
BseSI GKGCMC 1 cut(s) 130
BseXI GCAGC 6 cut(s) 367, 550, 1084, 1582, 1670, 1691
BsgI GTGCAG 1 cut(s) 387
Bsh1236I CGCG 1 cut(s) 1288
BshFI GGCC 2 cut(s) 1183, 1431
BshVI ATCGAT 1 cut(s) 1445
BsiHKAI GWGCWC 3 cut(s) 68, 1036, 1160
BsiSI CCGG 1 cut(s) 1314
BslFI GGGAC 2 cut(s) 1159, 1203
BslI CCNNNNNNNGG 2 cut(s) 284, 1128
BsmAI GTCTC 5 cut(s) 428, 744, 800, 1628, 1725
BsmFI GGGAC 2 cut(s) 1159, 1203
BsnI GGCC 2 cut(s) 1183, 1431
Bso31I GGTCTC 1 cut(s) 1628
Bsp1286I GDGCHC 4 cut(s) 68, 130, 1036, 1160
Bsp143I GATC 9 cut(s) 472, 532, 712, 793, 1043, 1056, 1348, 1446, 1693
BspACI CCGC 2 cut(s) 799, 991
BspANI GGCC 2 cut(s) 1183, 1431
BspDI ATCGAT 1 cut(s) 1445
BspFNI CGCG 1 cut(s) 1288
BspHI TCATGA 1 cut(s) 1048
BspLI GGNNCC 2 cut(s) 833, 1147
BspMAI CTGCAG 2 cut(s) 1660, 1711
BspPI GGATC 3 cut(s) 707, 1051, 1688
BspTI CTTAAG 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 1628
BsrDI GCAATG 1 cut(s) 1315
BsrI ACTGG 3 cut(s) 442, 1045, 1214
BssECI CCNNGG 4 cut(s) 111, 325, 336, 1314
BssMI GATC 9 cut(s) 472, 532, 712, 793, 1043, 1056, 1348, 1446, 1693
BssSI CACGAG 1 cut(s) 1665
BssT1I CCWWGG 2 cut(s) 111, 336
Bst2BI CACGAG 1 cut(s) 1665
Bst2UI CCWGG 6 cut(s) 173, 285, 717, 769, 1428, 1433
Bst4CI ACNGT 3 cut(s) 704, 982, 1592
BstAFI CTTAAG 1 cut(s) 203
BstAPI GCANNNNNTGC 2 cut(s) 674, 1715
BstBAI YACGTR 1 cut(s) 1079
BstC8I GCNNGC 3 cut(s) 561, 989, 1290
BstDEI CTNAG 1 cut(s) 1362
BstDSI CCRYGG 1 cut(s) 325
BstF5I GGATG 5 cut(s) 322, 504, 950, 1000, 1210
BstFNI CGCG 1 cut(s) 1288
BstKTI GATC 9 cut(s) 475, 535, 715, 796, 1046, 1059, 1351, 1449, 1696
BstMAI GTCTC 5 cut(s) 428, 744, 800, 1628, 1725
BstMBI GATC 9 cut(s) 472, 532, 712, 793, 1043, 1056, 1348, 1446, 1693
BstMWI GCNNNNNNNGC 5 cut(s) 665, 674, 1658, 1706, 1715
BstNI CCWGG 6 cut(s) 173, 285, 717, 769, 1428, 1433
BstNSI RCATGY 4 cut(s) 545, 1106, 1306, 1686
BstSCI CCNGG 7 cut(s) 171, 283, 715, 767, 1313, 1426, 1431
BstSFI CTRYAG 6 cut(s) 483, 663, 726, 1111, 1656, 1707
BstSLI GKGCMC 1 cut(s) 130
BstSNI TACGTA 1 cut(s) 1079
BstUI CGCG 1 cut(s) 1288
BstV1I GCAGC 6 cut(s) 367, 550, 1084, 1582, 1670, 1691
BstV2I GAAGAC 1 cut(s) 232
BstX2I RGATCY 2 cut(s) 532, 1043
BstXI CCANNNNNNTGG 1 cut(s) 1726
BstYI RGATCY 2 cut(s) 532, 1043
Bsu15I ATCGAT 1 cut(s) 1445
BsuRI GGCC 2 cut(s) 1183, 1431
BsuTUI ATCGAT 1 cut(s) 1445
BtgI CCRYGG 1 cut(s) 325
BtgZI GCGATG 1 cut(s) 798
BtsCI GGATG 5 cut(s) 322, 504, 950, 1000, 1210
BtsI GCAGTG 1 cut(s) 1347
BtsIMutI CAGTG 3 cut(s) 468, 1347, 1597
Cac8I GCNNGC 3 cut(s) 561, 989, 1290
CaiI CAGNNNCTG 1 cut(s) 1346
CciI TCATGA 1 cut(s) 1048
Cfr13I GGNCC 3 cut(s) 440, 832, 1146
ClaI ATCGAT 1 cut(s) 1445
CsiI ACCWGGT 1 cut(s) 283
Csp6I GTAC 5 cut(s) 23, 54, 93, 738, 1395
CviQI GTAC 5 cut(s) 23, 54, 93, 738, 1395
DdeI CTNAG 1 cut(s) 1362
DpnI GATC 9 cut(s) 474, 534, 714, 795, 1045, 1058, 1350, 1448, 1695
DpnII GATC 9 cut(s) 472, 532, 712, 793, 1043, 1056, 1348, 1446, 1693
DriI GACNNNNNGTC 1 cut(s) 287
Eam1105I GACNNNNNGTC 1 cut(s) 287
Ecl136II GAGCTC 2 cut(s) 1034, 1158
Eco105I TACGTA 1 cut(s) 1079
Eco130I CCWWGG 2 cut(s) 111, 336
Eco147I AGGCCT 2 cut(s) 1183, 1431
Eco24I GRGCYC 2 cut(s) 1036, 1160
Eco31I GGTCTC 1 cut(s) 1628
Eco47I GGWCC 3 cut(s) 440, 832, 1146
Eco53kI GAGCTC 2 cut(s) 1034, 1158
EcoICRI GAGCTC 2 cut(s) 1034, 1158
EcoRI GAATTC 2 cut(s) 731, 893
EcoRII CCWGG 6 cut(s) 171, 283, 715, 767, 1426, 1431
EcoT14I CCWWGG 2 cut(s) 111, 336
EcoT22I ATGCAT 2 cut(s) 193, 1504
EcoT38I GRGCYC 2 cut(s) 1036, 1160
ErhI CCWWGG 2 cut(s) 111, 336
FaqI GGGAC 2 cut(s) 1159, 1203
FauI CCCGC 1 cut(s) 984
FauNDI CATATG 1 cut(s) 1371
FbaI TGATCA 2 cut(s) 472, 1348
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 6 cut(s) 381, 564, 1073, 1596, 1659, 1680
FokI GGATG 5 cut(s) 329, 511, 937, 1007, 1217
FriOI GRGCYC 2 cut(s) 1036, 1160
Fsp4HI GCNGC 6 cut(s) 381, 564, 1073, 1596, 1659, 1680
FspBI CTAG 4 cut(s) 279, 504, 1199, 1325
GluI GCNGC 6 cut(s) 381, 564, 1073, 1596, 1659, 1680
GsuI CTGGAG 1 cut(s) 452
HaeIII GGCC 2 cut(s) 1183, 1431
HapII CCGG 1 cut(s) 1314
HincII GTYRAC 2 cut(s) 361, 1688
HindII GTYRAC 2 cut(s) 361, 1688
HindIII AAGCTT 2 cut(s) 600, 1353
HinfI GANTC 7 cut(s) 216, 608, 851, 880, 965, 1246, 1609
HpaI GTTAAC 1 cut(s) 361
HpaII CCGG 1 cut(s) 1314
HphI GGTGA 2 cut(s) 722, 1520
Hpy166II GTNNAC 6 cut(s) 215, 322, 361, 1108, 1474, 1688
Hpy188I TCNGA 7 cut(s) 392, 537, 836, 964, 1348, 1413, 1547
Hpy188III TCNNGA 7 cut(s) 431, 1049, 1188, 1222, 1586, 1665, 1749
Hpy8I GTNNAC 6 cut(s) 215, 322, 361, 1108, 1474, 1688
Hpy99I CGWCG 1 cut(s) 1402
HpyAV CCTTC 4 cut(s) 955, 969, 1089, 1194
HpyCH4III ACNGT 3 cut(s) 704, 982, 1592
HpyCH4IV ACGT 1 cut(s) 1078
HpyF10VI GCNNNNNNNGC 5 cut(s) 665, 674, 1658, 1706, 1715
HpyF3I CTNAG 1 cut(s) 1362
HpySE526I ACGT 1 cut(s) 1078
Ksp22I TGATCA 2 cut(s) 472, 1348
KspAI GTTAAC 1 cut(s) 361
Kzo9I GATC 9 cut(s) 472, 532, 712, 793, 1043, 1056, 1348, 1446, 1693
LmnI GCTCC 5 cut(s) 63, 946, 1039, 1132, 1155
Lsp1109I GCAGC 6 cut(s) 367, 550, 1084, 1582, 1670, 1691
LweI GCATC 5 cut(s) 489, 677, 775, 1417, 1670
MabI ACCWGGT 1 cut(s) 283
MaeI CTAG 4 cut(s) 279, 504, 1199, 1325
MaeII ACGT 1 cut(s) 1078
MaeIII GTNAC 5 cut(s) 152, 512, 572, 881, 1556
MalI GATC 9 cut(s) 474, 534, 714, 795, 1045, 1058, 1350, 1448, 1695
MboI GATC 9 cut(s) 472, 532, 712, 793, 1043, 1056, 1348, 1446, 1693
MboII GAAGA 2 cut(s) 232, 1066
MflI RGATCY 2 cut(s) 532, 1043
MhlI GDGCHC 4 cut(s) 68, 130, 1036, 1160
MluCI AATT 6 cut(s) 135, 370, 731, 893, 1086, 1422
MlyI GAGTC 5 cut(s) 210, 617, 889, 1255, 1618
MmeI TCCRAC 2 cut(s) 753, 1061
Mph1103I ATGCAT 2 cut(s) 193, 1504
MseI TTAA 4 cut(s) 204, 360, 1025, 1515
MslI CAYNNNNRTG 2 cut(s) 908, 936
MspA1I CMGCKG 1 cut(s) 991
MspCI CTTAAG 1 cut(s) 203
MspI CCGG 1 cut(s) 1314
MspR9I CCNGG 7 cut(s) 173, 285, 717, 769, 1315, 1428, 1433
MvaI CCWGG 6 cut(s) 173, 285, 717, 769, 1428, 1433
MvnI CGCG 1 cut(s) 1288
MwoI GCNNNNNNNGC 5 cut(s) 665, 674, 1658, 1706, 1715
NciI CCSGG 1 cut(s) 1315
NdeI CATATG 1 cut(s) 1371
NdeII GATC 9 cut(s) 472, 532, 712, 793, 1043, 1056, 1348, 1446, 1693
NlaIV GGNNCC 2 cut(s) 833, 1147
NmuCI GTSAC 3 cut(s) 512, 881, 1556
NsiI ATGCAT 2 cut(s) 193, 1504
NspI RCATGY 4 cut(s) 545, 1106, 1306, 1686
PagI TCATGA 1 cut(s) 1048
PceI AGGCCT 2 cut(s) 1183, 1431
PciI ACATGT 3 cut(s) 541, 1102, 1302
PfeI GAWTC 2 cut(s) 851, 965
PkrI GCNGC 6 cut(s) 382, 565, 1074, 1597, 1660, 1681
PleI GAGTC 5 cut(s) 210, 616, 888, 1254, 1617
PpsI GAGTC 5 cut(s) 210, 616, 888, 1254, 1617
Ppu21I YACGTR 1 cut(s) 1079
PscI ACATGT 3 cut(s) 541, 1102, 1302
PsiI TTATAA 1 cut(s) 1232
Psp124BI GAGCTC 2 cut(s) 1036, 1160
Psp6I CCWGG 6 cut(s) 171, 283, 715, 767, 1426, 1431
PspGI CCWGG 6 cut(s) 171, 283, 715, 767, 1426, 1431
PspN4I GGNNCC 2 cut(s) 833, 1147
PspPI GGNCC 3 cut(s) 440, 832, 1146
PstI CTGCAG 2 cut(s) 1660, 1711
PstNI CAGNNNCTG 1 cut(s) 1346
PsuI RGATCY 2 cut(s) 532, 1043
RsaI GTAC 5 cut(s) 24, 55, 94, 739, 1396
RsaNI GTAC 5 cut(s) 23, 54, 93, 738, 1395
RseI CAYNNNNRTG 2 cut(s) 908, 936
SacI GAGCTC 2 cut(s) 1036, 1160
SaqAI TTAA 4 cut(s) 204, 360, 1025, 1515
SatI GCNGC 6 cut(s) 381, 564, 1073, 1596, 1659, 1680
Sau3AI GATC 9 cut(s) 472, 532, 712, 793, 1043, 1056, 1348, 1446, 1693
Sau96I GGNCC 3 cut(s) 440, 832, 1146
ScaI AGTACT 2 cut(s) 24, 55
SchI GAGTC 5 cut(s) 210, 617, 889, 1255, 1618
ScrFI CCNGG 7 cut(s) 173, 285, 717, 769, 1315, 1428, 1433
SduI GDGCHC 4 cut(s) 68, 130, 1036, 1160
SexAI ACCWGGT 1 cut(s) 283
SfaNI GCATC 5 cut(s) 489, 677, 775, 1417, 1670
SfcI CTRYAG 6 cut(s) 483, 663, 726, 1111, 1656, 1707
SinI GGWCC 3 cut(s) 440, 832, 1146
SmiMI CAYNNNNRTG 2 cut(s) 908, 936
SmlI CTYRAG 2 cut(s) 203, 973
SmoI CTYRAG 2 cut(s) 203, 973
SnaBI TACGTA 1 cut(s) 1079
Sse9I AATT 6 cut(s) 135, 370, 731, 893, 1086, 1422
SseBI AGGCCT 2 cut(s) 1183, 1431
SsiI CCGC 2 cut(s) 799, 991
SspMI CTAG 4 cut(s) 279, 504, 1199, 1325
SstI GAGCTC 2 cut(s) 1036, 1160
StuI AGGCCT 2 cut(s) 1183, 1431
StyD4I CCNGG 7 cut(s) 171, 283, 715, 767, 1313, 1426, 1431
StyI CCWWGG 2 cut(s) 111, 336
TaaI ACNGT 3 cut(s) 704, 982, 1592
TaiI ACGT 1 cut(s) 1081
TaqI TCGA 7 cut(s) 166, 1059, 1093, 1265, 1445, 1456, 1587
TaqII GACCGA 1 cut(s) 1163
TasI AATT 6 cut(s) 135, 370, 731, 893, 1086, 1422
TatI WGTACW 2 cut(s) 22, 53
TfiI GAWTC 2 cut(s) 851, 965
Tru1I TTAA 4 cut(s) 204, 360, 1025, 1515
Tru9I TTAA 4 cut(s) 204, 360, 1025, 1515
TscAI CASTG 3 cut(s) 475, 1347, 1597
TseFI GTSAC 3 cut(s) 512, 881, 1556
TseI GCWGC 6 cut(s) 380, 563, 1072, 1595, 1658, 1679
Tsp45I GTSAC 3 cut(s) 512, 881, 1556
TspDTI ATGAA 8 cut(s) 232, 240, 448, 662, 864, 1187, 1292, 1380
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 3 cut(s) 475, 1347, 1597
Vha464I CTTAAG 1 cut(s) 203
VpaK11BI GGWCC 3 cut(s) 440, 832, 1146
XapI RAATTY 3 cut(s) 731, 893, 1422
XceI RCATGY 4 cut(s) 545, 1106, 1306, 1686
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 4 cut(s) 279, 504, 1199, 1325
ZrmI AGTACT 2 cut(s) 24, 55
Zsp2I ATGCAT 2 cut(s) 193, 1504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.