Rroxscaffold_3G00247020

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001085
Physical Location & Seq
Reverse (-)
40358516 .. 40361192
2677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00247020.1

Sequence Viewer

Length: 1236 bp
ATGAGAGGGCAGTACTGTTCCAGTGGAGTATTTTTGGTATTGCTTACCATCTTTGGTGTGAGCAGTGCTCAACTTCCCAAGTTGATGCCACAAGAATTTCCAGAGGATTTCATTATTCATGTTGATAGGGGCTCTAGCAACAGTGACACAAATGGAACAAGATGGGCAGTTCTTGTTGCTGGGTCTATGGGTTATGGAAACTATAGGCATCAGGCTGATGTGTGTCATGCATATCAAATTTTGAATAAAGGTGGACTGAAAGATGAAAACATCATTGTTTTCATGTACGATGACATTGCAAACAACCCTGAGAATCCAAGGCCTGGTGTCATCATCAACAAGCCCGACGGCGATGACGTCTACCATGGAGTTCCCAAGGATTACGCAGGAGAGAATGCTACTGCTGCTAATCTTTATGCTGTCATTCTTGGAGATAAATCTAATCTCTCTGGGGGAAGTGGCAAGGTTCTGAACAGCGGGCCAGACGACAATGTCTTTATCTATTACACCGACCATGGCGGGCCTGGGATCATTGGGATGCCTGAAGGCGATTATGTCTATGCCAATGATCTGGTAGATGTTCTGATAAAGAAGCATGAAGCTAATGCATACAAAAACATGGTATTTTACCTTGAAGCTTGTGAATCCGGGAGTATGTTCGAGGGTCTACTGCCGACAAATATAAGCATCTATGCAACCACAGCATCAAATGCAACTGAGAGTAGTTGGGGAACGTACTGCCCTGATATGACTCCTCCTCCACCTCCTGAATATGACACTTGCTTGGGTGATTTGTATAGTGTTTCTTGGATGGAGGATTGCAGTGACTTACAAGATTTGCGTCAAGAGTCTCTAGAAGAGCAATATCAAACGGTTAAATGGAGAACAGTAAATAATACTTATGGTTCTCATGTTATGCAATATGGAAATATGTATCAAACCTCAGATTTAGTATCCTTTTACTTGGGTGCAAATGATAACTCTTCCTCCATTGAAAGCAAATCGCCACAGTCAATCTTAGAAGTTGTTGACAATCGCGATGCAGATCTTCTTCATCTTTGGCACAAGTTCCAACGCGCACCCGCTGGCTCTGAAGTGAAGCAGGAAGCTAAAAGGGTCCTAGATGATGAAATTTCAAGTAGGCAGCGCATTGACCACAACATAAACCAAATCGAACAACTTGTGTTGGGATATTCAAGTAACTCTGTTCGTCTTGGGGGGCAAGCTCTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

411

Amino Acids

45.43

Weight (kDa)

4.59

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 54 - 323 3.8e-114 Peptidase C13 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
AatII GACGTC 1 cut(s) 360
AccB7I CCANNNNNTGG 1 cut(s) 323
AccI GTMKAC 2 cut(s) 360, 667
AccII CGCG 2 cut(s) 1038, 1077
AciI CCGC 3 cut(s) 477, 519, 1083
AclWI GGATC 1 cut(s) 536
AcsI RAATTY 3 cut(s) 95, 237, 1131
AcuI CTGAAG 2 cut(s) 564, 1113
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 3 cut(s) 14, 287, 737
AfiI CCNNNNNNNGG 1 cut(s) 323
AgsI TTSAA 5 cut(s) 244, 635, 995, 1137, 1197
AjnI CCWGG 2 cut(s) 322, 523
AluBI AGCT 4 cut(s) 602, 638, 1109, 1226
AluI AGCT 4 cut(s) 602, 638, 1109, 1226
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 1 cut(s) 855
AlwI GGATC 1 cut(s) 536
AoxI GGCC 3 cut(s) 320, 479, 521
ApeKI GCWGC 2 cut(s) 404, 1144
ApoI RAATTY 3 cut(s) 95, 237, 1131
AspLEI GCGC 2 cut(s) 1079, 1149
AspS9I GGNCC 3 cut(s) 479, 521, 1117
AsuC2I CCSGG 1 cut(s) 649
AsuHPI GGTGA 1 cut(s) 800
AvaII GGWCC 1 cut(s) 1117
BanII GRGCYC 1 cut(s) 134
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 391, 1156
BccI CCATC 3 cut(s) 56, 156, 805
BceAI ACGGC 1 cut(s) 364
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 2 cut(s) 324, 525
BciVI GTATCC 1 cut(s) 964
BcnI CCSGG 1 cut(s) 649
BcoDI GTCTC 1 cut(s) 855
BfaI CTAG 3 cut(s) 135, 854, 1121
BfmI CTRYAG 1 cut(s) 202
BfuI GTATCC 1 cut(s) 964
BglII AGATCT 1 cut(s) 1045
BisI GCNGC 2 cut(s) 405, 1145
BlsI GCNGC 2 cut(s) 406, 1146
BmcAI AGTACT 1 cut(s) 14
Bme1390I CCNGG 3 cut(s) 324, 525, 649
Bme18I GGWCC 1 cut(s) 1117
BmgT120I GGNCC 3 cut(s) 479, 521, 1117
BmiI GGNNCC 1 cut(s) 1118
BmrFI CCNGG 3 cut(s) 324, 525, 649
BmsI GCATC 6 cut(s) 75, 217, 528, 696, 713, 1030
BplI GAGNNNNNCTC 2 cut(s) 52, 84
BpuMI CCSGG 1 cut(s) 649
BsaBI GATNNNNATC 1 cut(s) 1044
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 5 cut(s) 317, 364, 375, 514, 524
BsaXI ACNNNNNCTCC 4 cut(s) 742, 772, 971, 1001
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse1I ACTGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 294
Bse8I GATNNNNATC 1 cut(s) 1044
BseBI CCWGG 2 cut(s) 324, 525
BseDI CCNNGG 5 cut(s) 317, 364, 375, 514, 524
BseGI GGATG 2 cut(s) 543, 816
BseJI GATNNNNATC 1 cut(s) 1044
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 3 cut(s) 300, 708, 957
BseNI ACTGG 1 cut(s) 21
BseRI GAGGAG 2 cut(s) 744, 747
BseXI GCAGC 2 cut(s) 391, 1156
BseYI CCCAGC 1 cut(s) 179
Bsh1236I CGCG 2 cut(s) 1038, 1077
BshFI GGCC 3 cut(s) 322, 481, 523
BsiHKAI GWGCWC 1 cut(s) 70
BsiSI CCGG 1 cut(s) 648
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 1 cut(s) 855
BsmI GAATGC 1 cut(s) 400
BsnI GGCC 3 cut(s) 322, 481, 523
Bsp1286I GDGCHC 2 cut(s) 70, 134
Bsp143I GATC 3 cut(s) 528, 568, 1045
Bsp19I CCATGG 2 cut(s) 364, 514
Bsp68I TCGCGA 1 cut(s) 1038
BspACI CCGC 3 cut(s) 477, 519, 1083
BspANI GGCC 3 cut(s) 322, 481, 523
BspCNI CTCAG 3 cut(s) 301, 709, 956
BspFNI CGCG 2 cut(s) 1038, 1077
BspLI GGNNCC 1 cut(s) 1118
BspPI GGATC 1 cut(s) 536
BspQI GCTCTTC 1 cut(s) 852
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 1 cut(s) 21
BssECI CCNNGG 5 cut(s) 317, 364, 375, 514, 524
BssMI GATC 3 cut(s) 528, 568, 1045
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 4 cut(s) 317, 364, 375, 514
Bst2UI CCWGG 2 cut(s) 324, 525
Bst4CI ACNGT 5 cut(s) 17, 143, 874, 889, 1011
Bst6I CTCTTC 2 cut(s) 852, 988
BstACI GRCGYC 1 cut(s) 357
BstAPI GCANNNNNTGC 1 cut(s) 710
BstC8I GCNNGC 4 cut(s) 479, 521, 1087, 1224
BstDEI CTNAG 4 cut(s) 309, 717, 943, 1018
BstDSI CCRYGG 2 cut(s) 364, 514
BstF5I GGATG 2 cut(s) 543, 816
BstFNI CGCG 2 cut(s) 1038, 1077
BstHHI GCGC 2 cut(s) 1079, 1149
BstKTI GATC 3 cut(s) 531, 571, 1048
BstMAI GTCTC 1 cut(s) 855
BstMBI GATC 3 cut(s) 528, 568, 1045
BstMWI GCNNNNNNNGC 3 cut(s) 404, 701, 710
BstNI CCWGG 2 cut(s) 324, 525
BstSCI CCNGG 3 cut(s) 322, 523, 647
BstSFI CTRYAG 1 cut(s) 202
BstUI CGCG 2 cut(s) 1038, 1077
BstV1I GCAGC 2 cut(s) 391, 1156
BstX2I RGATCY 1 cut(s) 1045
BstXI CCANNNNNNTGG 1 cut(s) 571
BstYI RGATCY 1 cut(s) 1045
BsuI GTATCC 1 cut(s) 964
BsuRI GGCC 3 cut(s) 322, 481, 523
BtgI CCRYGG 2 cut(s) 364, 514
BtgZI GCGATG 2 cut(s) 366, 1053
BtsCI GGATG 2 cut(s) 543, 816
BtsI GCAGTG 2 cut(s) 70, 829
BtsIMutI CAGTG 4 cut(s) 28, 70, 148, 829
BtuMI TCGCGA 1 cut(s) 1038
Cac8I GCNNGC 4 cut(s) 479, 521, 1087, 1224
CfoI GCGC 2 cut(s) 1079, 1149
Cfr13I GGNCC 3 cut(s) 479, 521, 1117
CseI GACGC 1 cut(s) 830
Csp6I GTAC 3 cut(s) 13, 286, 736
CviAII CATG 8 cut(s) 119, 227, 283, 365, 515, 596, 619, 911
CviQI GTAC 3 cut(s) 13, 286, 736
DdeI CTNAG 4 cut(s) 309, 717, 943, 1018
DpnI GATC 3 cut(s) 530, 570, 1047
DpnII GATC 3 cut(s) 528, 568, 1045
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
Eam1104I CTCTTC 2 cut(s) 852, 988
EarI CTCTTC 2 cut(s) 852, 988
Eco130I CCWWGG 4 cut(s) 317, 364, 375, 514
Eco147I AGGCCT 1 cut(s) 322
Eco24I GRGCYC 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 1117
Eco57I CTGAAG 2 cut(s) 564, 1113
EcoO109I RGGNCCY 1 cut(s) 1117
EcoRII CCWGG 2 cut(s) 322, 523
EcoT14I CCWWGG 4 cut(s) 317, 364, 375, 514
EcoT22I ATGCAT 2 cut(s) 232, 610
EcoT38I GRGCYC 1 cut(s) 134
ErhI CCWWGG 4 cut(s) 317, 364, 375, 514
FaeI CATG 8 cut(s) 122, 230, 286, 368, 518, 599, 622, 914
FatI CATG 8 cut(s) 118, 226, 282, 364, 514, 595, 618, 910
FauI CCCGC 3 cut(s) 470, 512, 1090
FblI GTMKAC 2 cut(s) 360, 667
Fnu4HI GCNGC 2 cut(s) 405, 1145
FokI GGATG 2 cut(s) 550, 823
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 2 cut(s) 405, 1145
FspBI CTAG 3 cut(s) 135, 854, 1121
GlaI GCGC 2 cut(s) 1078, 1148
GluI GCNGC 2 cut(s) 405, 1145
GsaI CCCAGC 1 cut(s) 183
HaeIII GGCC 3 cut(s) 322, 481, 523
HapII CCGG 1 cut(s) 648
HgaI GACGC 1 cut(s) 830
HhaI GCGC 2 cut(s) 1079, 1149
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 8 cut(s) 122, 230, 286, 368, 518, 599, 622, 914
Hin6I GCGC 2 cut(s) 1077, 1147
HinP1I GCGC 2 cut(s) 1077, 1147
HincII GTYRAC 1 cut(s) 1030
HindII GTYRAC 1 cut(s) 1030
HindIII AAGCTT 1 cut(s) 636
HinfI GANTC 4 cut(s) 313, 644, 751, 848
HpaII CCGG 1 cut(s) 648
HphI GGTGA 1 cut(s) 800
Hpy166II GTNNAC 4 cut(s) 254, 361, 668, 1030
Hpy188I TCNGA 4 cut(s) 471, 585, 946, 1093
Hpy188III TCNNGA 5 cut(s) 101, 767, 845, 854, 1037
Hpy8I GTNNAC 4 cut(s) 254, 361, 668, 1030
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 1 cut(s) 539
HpyCH4III ACNGT 5 cut(s) 17, 143, 874, 889, 1011
HpyCH4IV ACGT 2 cut(s) 357, 734
HpyCH4V TGCA 9 cut(s) 230, 299, 608, 695, 713, 822, 919, 971, 1043
HpyF10VI GCNNNNNNNGC 3 cut(s) 404, 701, 710
HpyF3I CTNAG 4 cut(s) 309, 717, 943, 1018
HpySE526I ACGT 2 cut(s) 357, 734
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 8 cut(s) 122, 230, 286, 368, 518, 599, 622, 914
HspAI GCGC 2 cut(s) 1077, 1147
Kzo9I GATC 3 cut(s) 528, 568, 1045
LguI GCTCTTC 1 cut(s) 852
Lsp1109I GCAGC 2 cut(s) 391, 1156
LweI GCATC 6 cut(s) 75, 217, 528, 696, 713, 1030
MaeI CTAG 3 cut(s) 135, 854, 1121
MaeII ACGT 2 cut(s) 357, 734
MaeIII GTNAC 3 cut(s) 143, 824, 1199
MalI GATC 3 cut(s) 530, 570, 1047
MboI GATC 3 cut(s) 528, 568, 1045
MboII GAAGA 4 cut(s) 869, 975, 1040, 1043
MflI RGATCY 1 cut(s) 1045
MhlI GDGCHC 2 cut(s) 70, 134
MluCI AATT 3 cut(s) 95, 237, 1131
MlyI GAGTC 2 cut(s) 745, 857
MmeI TCCRAC 1 cut(s) 1096
MnlI CCTC 8 cut(s) 97, 655, 765, 768, 774, 808, 952, 997
Mph1103I ATGCAT 2 cut(s) 232, 610
MseI TTAA 1 cut(s) 876
MslI CAYNNNNRTG 1 cut(s) 536
MspA1I CMGCKG 2 cut(s) 477, 1085
MspI CCGG 1 cut(s) 648
MspR9I CCNGG 3 cut(s) 324, 525, 649
Mva1269I GAATGC 1 cut(s) 400
MvaI CCWGG 2 cut(s) 324, 525
MvnI CGCG 2 cut(s) 1038, 1077
MwoI GCNNNNNNNGC 3 cut(s) 404, 701, 710
NciI CCSGG 1 cut(s) 649
NcoI CCATGG 2 cut(s) 364, 514
NdeII GATC 3 cut(s) 528, 568, 1045
NlaIII CATG 8 cut(s) 122, 230, 286, 368, 518, 599, 622, 914
NlaIV GGNNCC 1 cut(s) 1118
NmuCI GTSAC 2 cut(s) 143, 824
NruI TCGCGA 1 cut(s) 1038
NsiI ATGCAT 2 cut(s) 232, 610
PceI AGGCCT 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 852
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PctI GAATGC 1 cut(s) 400
PfeI GAWTC 2 cut(s) 313, 644
PflFI GACNNNGTC 1 cut(s) 491
PflMI CCANNNNNTGG 1 cut(s) 323
PfoI TCCNGGA 1 cut(s) 647
PkrI GCNGC 2 cut(s) 406, 1146
PleI GAGTC 2 cut(s) 745, 856
PpsI GAGTC 2 cut(s) 745, 856
PpuMI RGGWCCY 1 cut(s) 1117
Psp5II RGGWCCY 1 cut(s) 1117
Psp6I CCWGG 2 cut(s) 322, 523
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 2 cut(s) 322, 523
PspN4I GGNNCC 1 cut(s) 1118
PspPI GGNCC 3 cut(s) 479, 521, 1117
PspPPI RGGWCCY 1 cut(s) 1117
PsuI RGATCY 1 cut(s) 1045
PsyI GACNNNGTC 1 cut(s) 491
RruI TCGCGA 1 cut(s) 1038
RsaI GTAC 3 cut(s) 14, 287, 737
RsaNI GTAC 3 cut(s) 13, 286, 736
RseI CAYNNNNRTG 1 cut(s) 536
SapI GCTCTTC 1 cut(s) 852
SaqAI TTAA 1 cut(s) 876
SatI GCNGC 2 cut(s) 405, 1145
Sau3AI GATC 3 cut(s) 528, 568, 1045
Sau96I GGNCC 3 cut(s) 479, 521, 1117
ScaI AGTACT 1 cut(s) 14
SchI GAGTC 2 cut(s) 745, 857
ScrFI CCNGG 3 cut(s) 324, 525, 649
SduI GDGCHC 2 cut(s) 70, 134
SfaNI GCATC 6 cut(s) 75, 217, 528, 696, 713, 1030
SfcI CTRYAG 1 cut(s) 202
SinI GGWCC 1 cut(s) 1117
SmiMI CAYNNNNRTG 1 cut(s) 536
Sse9I AATT 3 cut(s) 95, 237, 1131
SseBI AGGCCT 1 cut(s) 322
SsiI CCGC 3 cut(s) 477, 519, 1083
SspMI CTAG 3 cut(s) 135, 854, 1121
StuI AGGCCT 1 cut(s) 322
StyD4I CCNGG 3 cut(s) 322, 523, 647
StyI CCWWGG 4 cut(s) 317, 364, 375, 514
TaaI ACNGT 5 cut(s) 17, 143, 874, 889, 1011
TaiI ACGT 2 cut(s) 360, 737
TaqI TCGA 2 cut(s) 660, 1173
TasI AATT 3 cut(s) 95, 237, 1131
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 2 cut(s) 313, 644
Tru1I TTAA 1 cut(s) 876
Tru9I TTAA 1 cut(s) 876
TscAI CASTG 4 cut(s) 28, 70, 148, 829
TseFI GTSAC 2 cut(s) 143, 824
TseI GCWGC 2 cut(s) 404, 1144
Tsp45I GTSAC 2 cut(s) 143, 824
TspDTI ATGAA 7 cut(s) 100, 107, 271, 279, 612, 1043, 1143
TspRI CASTG 4 cut(s) 28, 70, 148, 829
Tth111I GACNNNGTC 1 cut(s) 491
Van91I CCANNNNNTGG 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 1117
XapI RAATTY 3 cut(s) 95, 237, 1131
XbaI TCTAGA 1 cut(s) 853
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XmiI GTMKAC 2 cut(s) 360, 667
XspI CTAG 3 cut(s) 135, 854, 1121
ZraI GACGTC 1 cut(s) 358
ZrmI AGTACT 1 cut(s) 14
Zsp2I ATGCAT 2 cut(s) 232, 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.