MD04G1024900.v1.1

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001085
Physical Location & Seq
Reverse (-)
2877250 .. 2879750
2501 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1024900.v1.1.491

Sequence Viewer

Length: 1389 bp
ATGGCTAATCATGGTTATTGTGGGGTTTTACTTTCTCTTACTTTGTTTAGTTTGGCAATTCATGGAAGCTTCTGCTTCCCTGAAATTAATGGAGATAACAAGGGCTCTCCACGTACCACAACTGATAAGGGAAAAAGATGGGCAGTTCTGGTTGCAGGATCAAGTGGTTACGACAACTACAGGCACCAGGCTGACATATGCCATGCGTACCAGATACTTAAAAAAGGAGGACTGAAAGACGAGAACATCATCGTTTTCATGTACGATGATATCGCGTACAACTCGGAAAATCCTCGAAAAGGTGTCATCATCAACAAGCCAAATGGTCATGATGTTTATAAAGGAGTTCCCAAGGATTATACAGGAGATCATGTTAACGCACGCAATCTCTATGCTGTTATTCTCGGAGACAAAAGTGCTCTCACCGGAGGAAGTGGCAAGGTTCTAAGTAGCGGTCCGAATGACCATGTTTTCATATATTATGCAGACCATGGTTCTGTAGGATTACTTGGGATGCCCAGTGATTATGTTTATGCGAAAGATCTCATACGCGTACTACAAAAGAAGCATGCTTCTAAAGGTTACAAAAGCATGGTATTTTACATTGAAGCTTGCGAGGCAGGGAGCATGTTTGAGGGCCTTCTTTCAAGTAATCTGAATATTTATGCTACCACTGCGTCAAATGCAGAAGAGAGTAGTTATGGAACATATTGTCCTGGTGACCCATCAGTTCCTGAAGAGTTTGATACTTGTTTGGGAGACTTGTATAGCATTTCCTGGATGGAGGATTGTGACATAAGTGATTTGCATAAAGAAACTTTGGAGAATCAATATGAAACGGTTCGAAGAAGAACAACTAATTCGCATGTTATGCAGTACGGAGATATGAGTCATAAACAGGAGTTCCTCTTTGCTTACATGGGTACAGATCTTTCTAATCGTAGCCATACTTCCACCAGCGATATCTCTTCACCCTCGATCTCAAGGGCTGTTGACCAACGTGACACGAAGCTCCTCTACTTTCAACAAAAGCTGAAAAGAGCTCCTACTGGATCTCAAGAGAAACAGGGTGCTCAGAAGCAGCTGCTGCTTGAAATTGCTCATAGGAAAACCGTGGACTATAGCATAACGAAACTAGGGGAGGTTTTGTTTGGACACGAGAAGAGCTCAAACGTTTTAATGAATGTTAGGCCACAGGGCCAACCTGTGGTAGATAATTGGGACTGCTTCAAGAACTTTCTGAATATTTATGAGAAGTACTGTGGACATTTATCTGCATACGGAATGAAGTACACGCGAGCTATAGCCAACATTTGCAATGCTGGGATTACCACAGAGAAAATGGTTGCAGCATCTGATCAAACTTGTGCCAACAAACCCAATGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

463

Amino Acids

51.33

Weight (kDa)

6.6

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 46 - 309 1.6e-119 Peptidase C13 family
Legum_prodom PF20985 360 - 456 1e-36 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 183
AccB7I CCANNNNNTGG 1 cut(s) 1207
AccII CGCG 3 cut(s) 275, 552, 1297
AciI CCGC 1 cut(s) 453
AclI AACGTT 1 cut(s) 1173
AclWI GGATC 2 cut(s) 166, 1060
AcuI CTGAAG 1 cut(s) 756
AfaI GTAC 9 cut(s) 115, 209, 263, 278, 555, 878, 925, 1259, 1292
AfiI CCNNNNNNNGG 2 cut(s) 299, 1207
AflIII ACRYGT 1 cut(s) 550
AgsI TTSAA 5 cut(s) 608, 648, 1025, 1094, 1231
AjnI CCWGG 3 cut(s) 186, 715, 776
AloI GAACNNNNNNTCC 2 cut(s) 697, 729
AluBI AGCT 8 cut(s) 69, 611, 1012, 1033, 1043, 1084, 1167, 1301
AluI AGCT 8 cut(s) 69, 611, 1012, 1033, 1043, 1084, 1167, 1301
Alw21I GWGCWC 4 cut(s) 421, 1045, 1075, 1169
Alw26I GTCTC 2 cut(s) 402, 753
AlwI GGATC 2 cut(s) 166, 1060
AlwNI CAGNNNCTG 3 cut(s) 734, 1087, 1355
AoxI GGCC 3 cut(s) 637, 1190, 1198
ApeKI GCWGC 4 cut(s) 1081, 1084, 1087, 1349
ArsI GACNNNNNNTTYG 1 cut(s) 1368
AseI ATTAAT 1 cut(s) 87
Asp700I GAANNNNTTC 1 cut(s) 840
AspS9I GGNCC 3 cut(s) 455, 637, 1198
AsuHPI GGTGA 3 cut(s) 415, 731, 963
AsuII TTCGAA 1 cut(s) 844
AvaII GGWCC 1 cut(s) 455
BanI GGYRCC 1 cut(s) 183
BanII GRGCYC 3 cut(s) 107, 1045, 1169
BarI GAAGNNNNNNTAC 4 cut(s) 934, 966, 1001, 1033
BauI CACGAG 1 cut(s) 1157
Bbv12I GWGCWC 4 cut(s) 421, 1045, 1075, 1169
BbvI GCAGC 4 cut(s) 1071, 1074, 1093, 1361
BccI CCATC 3 cut(s) 132, 733, 775
BciT130I CCWGG 3 cut(s) 188, 717, 778
BclI TGATCA 1 cut(s) 1357
BcoDI GTCTC 2 cut(s) 402, 753
BfaI CTAG 2 cut(s) 1136, 1387
BfmI CTRYAG 4 cut(s) 178, 498, 1120, 1302
BglII AGATCT 2 cut(s) 541, 928
BisI GCNGC 4 cut(s) 1082, 1085, 1088, 1350
BlsI GCNGC 4 cut(s) 1083, 1086, 1089, 1351
BmcAI AGTACT 1 cut(s) 1259
Bme1390I CCNGG 3 cut(s) 188, 717, 778
Bme18I GGWCC 1 cut(s) 455
BmgT120I GGNCC 3 cut(s) 455, 637, 1198
BmiI GGNNCC 1 cut(s) 185
BmrFI CCNGG 3 cut(s) 188, 717, 778
BmrI ACTGGG 1 cut(s) 513
BmsI GCATC 2 cut(s) 504, 1361
BmuI ACTGGG 1 cut(s) 513
BplI GAGNNNNNCTC 2 cut(s) 1151, 1183
Bpu14I TTCGAA 1 cut(s) 844
BpuEI CTTGAG 2 cut(s) 967, 1041
BsaAI YACGTR 1 cut(s) 113
BsaJI CCNNGG 3 cut(s) 351, 490, 1113
BsaWI WCCGGW 1 cut(s) 425
BsaXI ACNNNNNCTCC 4 cut(s) 357, 387, 1133, 1163
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 1207
Bse1I ACTGG 2 cut(s) 519, 1054
Bse3DI GCAATG 1 cut(s) 1324
BseBI CCWGG 3 cut(s) 188, 717, 778
BseDI CCNNGG 3 cut(s) 351, 490, 1113
BseGI GGATG 2 cut(s) 519, 786
BseLI CCNNNNNNNGG 2 cut(s) 299, 1207
BseMI GCAATG 1 cut(s) 1324
BseMII CTCAG 1 cut(s) 1088
BseNI ACTGG 2 cut(s) 519, 1054
BseRI GAGGAG 1 cut(s) 1004
BseXI GCAGC 4 cut(s) 1071, 1074, 1093, 1361
BseYI CCCAGC 1 cut(s) 1322
Bsh1236I CGCG 3 cut(s) 275, 552, 1297
BshFI GGCC 3 cut(s) 639, 1192, 1200
BshNI GGYRCC 1 cut(s) 183
BsiHKAI GWGCWC 4 cut(s) 421, 1045, 1075, 1169
BsiSI CCGG 1 cut(s) 426
BslFI GGGAC 1 cut(s) 1235
BslI CCNNNNNNNGG 2 cut(s) 299, 1207
BsmAI GTCTC 2 cut(s) 402, 753
BsmFI GGGAC 1 cut(s) 1235
BsnI GGCC 3 cut(s) 639, 1192, 1200
Bsp119I TTCGAA 1 cut(s) 844
Bsp1286I GDGCHC 5 cut(s) 107, 421, 1045, 1075, 1169
Bsp143I GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
Bsp19I CCATGG 1 cut(s) 490
BspACI CCGC 1 cut(s) 453
BspANI GGCC 3 cut(s) 639, 1192, 1200
BspCNI CTCAG 1 cut(s) 1087
BspFNI CGCG 3 cut(s) 275, 552, 1297
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 1 cut(s) 185
BspPI GGATC 2 cut(s) 166, 1060
BspQI GCTCTTC 1 cut(s) 1157
BspT104I TTCGAA 1 cut(s) 844
BspT107I GGYRCC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 1324
BsrI ACTGG 2 cut(s) 519, 1054
BssECI CCNNGG 3 cut(s) 351, 490, 1113
BssMI GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
BssSI CACGAG 1 cut(s) 1157
BssT1I CCWWGG 2 cut(s) 351, 490
Bst2BI CACGAG 1 cut(s) 1157
Bst2UI CCWGG 3 cut(s) 188, 717, 778
Bst4CI ACNGT 3 cut(s) 841, 1114, 1262
Bst6I CTCTTC 4 cut(s) 684, 732, 973, 1157
BstAPI GCANNNNNTGC 2 cut(s) 871, 1087
BstBAI YACGTR 1 cut(s) 113
BstBI TTCGAA 1 cut(s) 844
BstC8I GCNNGC 4 cut(s) 382, 570, 613, 1299
BstDEI CTNAG 2 cut(s) 446, 1074
BstDSI CCRYGG 2 cut(s) 490, 1113
BstEII GGTNACC 1 cut(s) 719
BstENI CCTNNNNNAGG 1 cut(s) 297
BstF5I GGATG 2 cut(s) 519, 786
BstFNI CGCG 3 cut(s) 275, 552, 1297
BstKTI GATC 7 cut(s) 161, 370, 544, 931, 981, 1055, 1360
BstMAI GTCTC 2 cut(s) 402, 753
BstMBI GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
BstMWI GCNNNNNNNGC 5 cut(s) 617, 674, 683, 871, 1087
BstNI CCWGG 3 cut(s) 188, 717, 778
BstNSI RCATGY 3 cut(s) 572, 631, 869
BstPI GGTNACC 1 cut(s) 719
BstSCI CCNGG 3 cut(s) 186, 715, 776
BstSFI CTRYAG 4 cut(s) 178, 498, 1120, 1302
BstUI CGCG 3 cut(s) 275, 552, 1297
BstV1I GCAGC 4 cut(s) 1071, 1074, 1093, 1361
BstX2I RGATCY 3 cut(s) 541, 928, 1052
BstYI RGATCY 3 cut(s) 541, 928, 1052
BsuRI GGCC 3 cut(s) 639, 1192, 1200
BtgI CCRYGG 2 cut(s) 490, 1113
BtsCI GGATG 2 cut(s) 519, 786
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 2 cut(s) 526, 672
Cac8I GCNNGC 4 cut(s) 382, 570, 613, 1299
CaiI CAGNNNCTG 3 cut(s) 734, 1087, 1355
CciI TCATGA 1 cut(s) 328
Cfr13I GGNCC 3 cut(s) 455, 637, 1198
CpoI CGGWCCG 1 cut(s) 455
CseI GACGC 1 cut(s) 666
Csp6I GTAC 9 cut(s) 114, 208, 262, 277, 554, 877, 924, 1258, 1291
CspI CGGWCCG 1 cut(s) 455
CviQI GTAC 9 cut(s) 114, 208, 262, 277, 554, 877, 924, 1258, 1291
DdeI CTNAG 2 cut(s) 446, 1074
DpnI GATC 7 cut(s) 160, 369, 543, 930, 980, 1054, 1359
DpnII GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
Eam1104I CTCTTC 4 cut(s) 684, 732, 973, 1157
EarI CTCTTC 4 cut(s) 684, 732, 973, 1157
Ecl136II GAGCTC 2 cut(s) 1043, 1167
Eco130I CCWWGG 2 cut(s) 351, 490
Eco24I GRGCYC 3 cut(s) 107, 1045, 1169
Eco32I GATATC 2 cut(s) 271, 964
Eco47I GGWCC 1 cut(s) 455
Eco53kI GAGCTC 2 cut(s) 1043, 1167
Eco57I CTGAAG 1 cut(s) 756
Eco91I GGTNACC 1 cut(s) 719
EcoICRI GAGCTC 2 cut(s) 1043, 1167
EcoNI CCTNNNNNAGG 1 cut(s) 297
EcoO109I RGGNCCY 1 cut(s) 637
EcoO65I GGTNACC 1 cut(s) 719
EcoRII CCWGG 3 cut(s) 186, 715, 776
EcoRV GATATC 2 cut(s) 271, 964
EcoT14I CCWWGG 2 cut(s) 351, 490
EcoT38I GRGCYC 3 cut(s) 107, 1045, 1169
ErhI CCWWGG 2 cut(s) 351, 490
FaqI GGGAC 1 cut(s) 1235
FauNDI CATATG 1 cut(s) 197
FbaI TGATCA 1 cut(s) 1357
Fnu4HI GCNGC 4 cut(s) 1082, 1085, 1088, 1350
FokI GGATG 2 cut(s) 526, 793
FriOI GRGCYC 3 cut(s) 107, 1045, 1169
Fsp4HI GCNGC 4 cut(s) 1082, 1085, 1088, 1350
FspBI CTAG 2 cut(s) 1136, 1387
GluI GCNGC 4 cut(s) 1082, 1085, 1088, 1350
GsaI CCCAGC 1 cut(s) 1326
HaeIII GGCC 3 cut(s) 639, 1192, 1200
HapII CCGG 1 cut(s) 426
HgaI GACGC 1 cut(s) 666
HincII GTYRAC 2 cut(s) 376, 994
HindII GTYRAC 2 cut(s) 376, 994
HindIII AAGCTT 2 cut(s) 67, 609
HinfI GANTC 2 cut(s) 826, 889
HpaI GTTAAC 1 cut(s) 376
HpaII CCGG 1 cut(s) 426
HphI GGTGA 3 cut(s) 415, 731, 963
Hpy166II GTNNAC 5 cut(s) 376, 994, 1117, 1265, 1293
Hpy188I TCNGA 7 cut(s) 286, 407, 459, 657, 1077, 1242, 1357
Hpy188III TCNNGA 4 cut(s) 329, 734, 1058, 1231
Hpy8I GTNNAC 5 cut(s) 376, 994, 1117, 1265, 1293
HpyAV CCTTC 1 cut(s) 650
HpyCH4III ACNGT 3 cut(s) 841, 1114, 1262
HpyCH4IV ACGT 3 cut(s) 112, 1000, 1173
HpyCH4V TGCA 8 cut(s) 155, 485, 686, 808, 874, 1277, 1317, 1349
HpyF10VI GCNNNNNNNGC 5 cut(s) 617, 674, 683, 871, 1087
HpyF3I CTNAG 2 cut(s) 446, 1074
HpySE526I ACGT 3 cut(s) 112, 1000, 1173
Ksp22I TGATCA 1 cut(s) 1357
KspAI GTTAAC 1 cut(s) 376
Kzo9I GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
LguI GCTCTTC 1 cut(s) 1157
LmnI GCTCC 3 cut(s) 624, 1017, 1048
Lsp1109I GCAGC 4 cut(s) 1071, 1074, 1093, 1361
LweI GCATC 2 cut(s) 504, 1361
MaeI CTAG 2 cut(s) 1136, 1387
MaeII ACGT 3 cut(s) 112, 1000, 1173
MaeIII GTNAC 5 cut(s) 167, 581, 719, 791, 1001
MalI GATC 7 cut(s) 160, 369, 543, 930, 980, 1054, 1359
MboI GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
MboII GAAGA 6 cut(s) 701, 749, 858, 861, 960, 1174
MflI RGATCY 3 cut(s) 541, 928, 1052
MhlI GDGCHC 5 cut(s) 107, 421, 1045, 1075, 1169
MluCI AATT 5 cut(s) 57, 84, 859, 1095, 1216
MluI ACGCGT 1 cut(s) 550
MlyI GAGTC 1 cut(s) 898
MroXI GAANNNNTTC 1 cut(s) 840
MseI TTAA 4 cut(s) 87, 219, 375, 1178
MspA1I CMGCKG 1 cut(s) 1084
MspI CCGG 1 cut(s) 426
MspR9I CCNGG 3 cut(s) 188, 717, 778
MvaI CCWGG 3 cut(s) 188, 717, 778
MvnI CGCG 3 cut(s) 275, 552, 1297
MwoI GCNNNNNNNGC 5 cut(s) 617, 674, 683, 871, 1087
NcoI CCATGG 1 cut(s) 490
NdeI CATATG 1 cut(s) 197
NdeII GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
NlaIV GGNNCC 1 cut(s) 185
NmuCI GTSAC 3 cut(s) 719, 791, 1001
NspI RCATGY 3 cut(s) 572, 631, 869
NspV TTCGAA 1 cut(s) 844
PaeI GCATGC 1 cut(s) 572
PagI TCATGA 1 cut(s) 328
PciSI GCTCTTC 1 cut(s) 1157
PdmI GAANNNNTTC 1 cut(s) 840
PfeI GAWTC 1 cut(s) 826
PflMI CCANNNNNTGG 1 cut(s) 1207
PfoI TCCNGGA 1 cut(s) 776
PkrI GCNGC 4 cut(s) 1083, 1086, 1089, 1351
PleI GAGTC 1 cut(s) 897
PpsI GAGTC 1 cut(s) 897
Ppu21I YACGTR 1 cut(s) 113
PshBI ATTAAT 1 cut(s) 87
PsiI TTATAA 1 cut(s) 339
Psp124BI GAGCTC 2 cut(s) 1045, 1169
Psp1406I AACGTT 1 cut(s) 1173
Psp6I CCWGG 3 cut(s) 186, 715, 776
PspEI GGTNACC 1 cut(s) 719
PspFI CCCAGC 1 cut(s) 1322
PspGI CCWGG 3 cut(s) 186, 715, 776
PspN4I GGNNCC 1 cut(s) 185
PspPI GGNCC 3 cut(s) 455, 637, 1198
PstNI CAGNNNCTG 3 cut(s) 734, 1087, 1355
PsuI RGATCY 3 cut(s) 541, 928, 1052
PvuII CAGCTG 1 cut(s) 1084
RsaI GTAC 9 cut(s) 115, 209, 263, 278, 555, 878, 925, 1259, 1292
RsaNI GTAC 9 cut(s) 114, 208, 262, 277, 554, 877, 924, 1258, 1291
Rsr2I CGGWCCG 1 cut(s) 455
RsrII CGGWCCG 1 cut(s) 455
SacI GAGCTC 2 cut(s) 1045, 1169
SapI GCTCTTC 1 cut(s) 1157
SaqAI TTAA 4 cut(s) 87, 219, 375, 1178
SatI GCNGC 4 cut(s) 1082, 1085, 1088, 1350
Sau3AI GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
Sau96I GGNCC 3 cut(s) 455, 637, 1198
ScaI AGTACT 1 cut(s) 1259
SchI GAGTC 1 cut(s) 898
ScrFI CCNGG 3 cut(s) 188, 717, 778
SduI GDGCHC 5 cut(s) 107, 421, 1045, 1075, 1169
SfaNI GCATC 2 cut(s) 504, 1361
SfcI CTRYAG 4 cut(s) 178, 498, 1120, 1302
SfuI TTCGAA 1 cut(s) 844
SinI GGWCC 1 cut(s) 455
SmlI CTYRAG 2 cut(s) 982, 1056
SmoI CTYRAG 2 cut(s) 982, 1056
SphI GCATGC 1 cut(s) 572
Sse9I AATT 5 cut(s) 57, 84, 859, 1095, 1216
SsiI CCGC 1 cut(s) 453
SspI AATATT 2 cut(s) 661, 1246
SspMI CTAG 2 cut(s) 1136, 1387
SstI GAGCTC 2 cut(s) 1045, 1169
StyD4I CCNGG 3 cut(s) 186, 715, 776
StyI CCWWGG 2 cut(s) 351, 490
TaaI ACNGT 3 cut(s) 841, 1114, 1262
TaiI ACGT 3 cut(s) 115, 1003, 1176
TaqI TCGA 3 cut(s) 295, 844, 977
TasI AATT 5 cut(s) 57, 84, 859, 1095, 1216
TatI WGTACW 2 cut(s) 1257, 1290
TfiI GAWTC 1 cut(s) 826
Tru1I TTAA 4 cut(s) 87, 219, 375, 1178
Tru9I TTAA 4 cut(s) 87, 219, 375, 1178
TscAI CASTG 2 cut(s) 526, 679
TseFI GTSAC 3 cut(s) 719, 791, 1001
TseI GCWGC 4 cut(s) 1081, 1084, 1087, 1349
Tsp45I GTSAC 3 cut(s) 719, 791, 1001
TspDTI ATGAA 6 cut(s) 50, 247, 463, 849, 1196, 1301
TspGWI ACGGA 2 cut(s) 894, 1296
TspRI CASTG 2 cut(s) 526, 679
Van91I CCANNNNNTGG 1 cut(s) 1207
VpaK11BI GGWCC 1 cut(s) 455
VspI ATTAAT 1 cut(s) 87
XagI CCTNNNNNAGG 1 cut(s) 297
XceI RCATGY 3 cut(s) 572, 631, 869
XcmI CCANNNNNNNNNTGG 1 cut(s) 1339
XmnI GAANNNNTTC 1 cut(s) 840
XspI CTAG 2 cut(s) 1136, 1387
ZrmI AGTACT 1 cut(s) 1259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.