FvH4_6g36630

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001085
Physical Location & Seq
Reverse (-)
28821796 .. 28824931
3136 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g36630.t1

Sequence Viewer

Length: 1383 bp
ATGAGTATCCAAGGTTTACTGGTACTTCTTTTCCTGTCTGTTTTGGTTGAGAGTACTATTGGAAGCACCCTTGGAGAAAATGGCTCTCTAAGTACCACTGATCATCATAAGTCGAAAGGAACAAGATGGGCACTTCTAATTGCCGGTTCGAGTGGTTACGAGAACTATCGACACCAGGCTGATGTATGCCATGCATATCAGATACTCAAGAAAGGCGGACTCAAAGATGAAAACATCGTTGTCTTCATGTATGATGATATTGCCAACAGCCCAGAAAACCCTAGACCTGGTGTCATTATTAACAAGCCAAATGGCAAGGACGTCTACCATGGAGTTCCCAAGGATTACACAGGAGATGCTGTTAACTCATCAAATTTCTATGCTGCTATTCTGGGAGACAAAGCTGCAATCAAAGGAGGAAGTGGCAAGGTTCTGGAGACTGGTCCAGATGACCATATTTTCATATATTACACTGATCATGGTGCTGCAGGACTTCTTGGGATGCCTAGTGACGAGGACGCTGTTTATGCGAAAGATCTAATGCGTGTGCTGAAAAAGAAGCACGCTGCTAAAGGTTTCAAAAGCATGGTGTTTTACCTAGAAGCTTGTGAGTCTGGGAGTATGTTTGAGGGTTTGCTTCCAAAGAATATAAACATATATGCTACTACAGCAGCAAATGGAGAGGAGAGCAGTTATGCAACATACTGTTCTGGAGATGTTCCTGAAAAAATCGGTACTTGTTTGGGAGACTTGTATAGTATTTCCTGGTTGGAGGATTGCGACGCACATGATCTGCACAAAGAGACTTTTGAGGTTCAATATGAAAGGGTTCGGAACAGAACAGATGAATCCCATGTTATGCAGTATGGAGATATGAGTTACATAAAGCAGTTCCTGTACACTTACATGGGGGCAACTTCTAAAGCTCATAGCCATGCTCCCCCTGCCCTCACAGCTTCGCCTTCGGGATCAATCTCAAGGGCTGTTAGCCAGCGCGATGCAGCCCTCTTTTATTTTCTGTATAAGGTAAACAGAGCTCCAACTAACTCTCATGAGAAGCTCGAAGCTAAAAAGCAGCTATATAATGAAATTGCTGGAAGGAAACGTGTAGATGATAGCATAACAAAAATGGGAGAGCTTCTGTTTGGGACCGAGAAGGGCTCAGAATTACTAATGAAGGTCAGGCCTTCAGGACAACCTCTAGTGGATGACTGGGAGTGCTTCAAGATGTTTGTTAGGACTTATGAGAGTCGCTGCGGAAAGCTATCGACATATGGAATGAAGTACACGCGAGCTTTTGCCAACATGTGCAATGCCGGGATCAGCCTAGAGAAACTGGTTGCAGTGTCTGATCAAGCTTGTTCTAAGAAACCTCATGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

461

Amino Acids

50.76

Weight (kDa)

6.57

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 42 - 304 6.8e-117 Peptidase C13 family
Legum_prodom PF20985 358 - 454 2.2e-37 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 324
AccI GTMKAC 1 cut(s) 324
AccII CGCG 2 cut(s) 996, 1291
AciI CCGC 2 cut(s) 216, 1257
AclWI GGATC 2 cut(s) 976, 1328
AcsI RAATTY 1 cut(s) 373
AcuI CTGAAG 1 cut(s) 1173
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 6 cut(s) 24, 55, 94, 736, 899, 1286
AfiI CCNNNNNNNGG 1 cut(s) 287
AflIII ACRYGT 2 cut(s) 1105, 1305
AgsI TTSAA 3 cut(s) 580, 818, 1225
AhdI GACNNNNNGTC 1 cut(s) 290
AjnI CCWGG 3 cut(s) 174, 286, 764
Alw21I GWGCWC 1 cut(s) 1039
Alw26I GTCTC 4 cut(s) 390, 431, 741, 797
AlwI GGATC 2 cut(s) 976, 1328
AlwNI CAGNNNCTG 2 cut(s) 895, 1349
AoxI GGCC 1 cut(s) 1184
ApeKI GCWGC 8 cut(s) 383, 404, 485, 566, 671, 1001, 1075, 1254
ApoI RAATTY 1 cut(s) 373
Asp700I GAANNNNTTC 1 cut(s) 828
AspLEI GCGC 1 cut(s) 996
AspS9I GGNCC 2 cut(s) 443, 1149
AsuC2I CCSGG 1 cut(s) 1318
AvaII GGWCC 2 cut(s) 443, 1149
BaeGI GKGCMC 1 cut(s) 133
BanII GRGCYC 2 cut(s) 1039, 1163
BbsI GAAGAC 1 cut(s) 235
Bbv12I GWGCWC 1 cut(s) 1039
BbvI GCAGC 8 cut(s) 370, 391, 472, 553, 683, 1013, 1087, 1241
BccI CCATC 1 cut(s) 120
BciT130I CCWGG 3 cut(s) 176, 288, 766
BciVI GTATCC 1 cut(s) 17
BclI TGATCA 3 cut(s) 100, 475, 1351
BcnI CCSGG 1 cut(s) 1318
BcoDI GTCTC 4 cut(s) 390, 431, 741, 797
BfaI CTAG 5 cut(s) 282, 507, 599, 1202, 1328
BfmI CTRYAG 2 cut(s) 486, 666
BfuI GTATCC 1 cut(s) 17
BglII AGATCT 1 cut(s) 535
BisI GCNGC 8 cut(s) 384, 405, 486, 567, 672, 1002, 1076, 1255
BlsI GCNGC 8 cut(s) 385, 406, 487, 568, 673, 1003, 1077, 1256
BmcAI AGTACT 1 cut(s) 55
Bme1390I CCNGG 4 cut(s) 176, 288, 766, 1318
Bme18I GGWCC 2 cut(s) 443, 1149
BmeRI GACNNNNNGTC 1 cut(s) 290
BmgT120I GGNCC 2 cut(s) 443, 1149
BmiI GGNNCC 1 cut(s) 1150
BmrFI CCNGG 4 cut(s) 176, 288, 766, 1318
BmrI ACTGGG 1 cut(s) 1222
BmsI GCATC 3 cut(s) 346, 492, 988
BmuI ACTGGG 1 cut(s) 1222
BpiI GAAGAC 1 cut(s) 235
BplI GAGNNNNNCTC 2 cut(s) 1145, 1177
BpmI CTGGAG 2 cut(s) 455, 732
BpuEI CTTGAG 2 cut(s) 191, 961
BpuMI CCSGG 1 cut(s) 1318
BsaHI GRCGYC 1 cut(s) 321
BsaJI CCNNGG 4 cut(s) 10, 70, 328, 339
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 4 cut(s) 24, 445, 1217, 1341
Bse3DI GCAATG 1 cut(s) 1318
BseBI CCWGG 3 cut(s) 176, 288, 766
BseDI CCNNGG 4 cut(s) 10, 70, 328, 339
BseGI GGATG 2 cut(s) 507, 1213
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMI GCAATG 1 cut(s) 1318
BseMII CTCAG 1 cut(s) 1176
BseNI ACTGG 4 cut(s) 24, 445, 1217, 1341
BseRI GAGGAG 1 cut(s) 698
BseSI GKGCMC 1 cut(s) 133
BseXI GCAGC 8 cut(s) 370, 391, 472, 553, 683, 1013, 1087, 1241
BsgI GTGCAG 1 cut(s) 779
Bsh1236I CGCG 2 cut(s) 996, 1291
BshFI GGCC 1 cut(s) 1186
BsiHKAI GWGCWC 1 cut(s) 1039
BsiSI CCGG 2 cut(s) 144, 1317
BslFI GGGAC 1 cut(s) 1162
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 4 cut(s) 390, 431, 741, 797
BsmFI GGGAC 1 cut(s) 1162
BsnI GGCC 1 cut(s) 1186
Bsp1286I GDGCHC 3 cut(s) 133, 1039, 1163
Bsp1407I TGTACA 1 cut(s) 897
Bsp143I GATC 7 cut(s) 100, 475, 535, 790, 968, 1320, 1351
Bsp19I CCATGG 1 cut(s) 328
BspACI CCGC 2 cut(s) 216, 1257
BspANI GGCC 1 cut(s) 1186
BspCNI CTCAG 1 cut(s) 1175
BspFNI CGCG 2 cut(s) 996, 1291
BspHI TCATGA 1 cut(s) 1051
BspLI GGNNCC 1 cut(s) 1150
BspMAI CTGCAG 1 cut(s) 490
BspPI GGATC 2 cut(s) 976, 1328
BsrDI GCAATG 1 cut(s) 1318
BsrFI RCCGGY 1 cut(s) 143
BsrGI TGTACA 1 cut(s) 897
BsrI ACTGG 4 cut(s) 24, 445, 1217, 1341
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 4 cut(s) 10, 70, 328, 339
BssMI GATC 7 cut(s) 100, 475, 535, 790, 968, 1320, 1351
BssNI GRCGYC 1 cut(s) 321
BssT1I CCWWGG 4 cut(s) 10, 70, 328, 339
Bst2UI CCWGG 3 cut(s) 176, 288, 766
Bst4CI ACNGT 1 cut(s) 707
BstACI GRCGYC 1 cut(s) 321
BstAUI TGTACA 1 cut(s) 897
BstC8I GCNNGC 3 cut(s) 564, 992, 1293
BstDEI CTNAG 3 cut(s) 89, 1162, 1365
BstDSI CCRYGG 1 cut(s) 328
BstF5I GGATG 2 cut(s) 507, 1213
BstFNI CGCG 2 cut(s) 996, 1291
BstHHI GCGC 1 cut(s) 996
BstKTI GATC 7 cut(s) 103, 478, 538, 793, 971, 1323, 1354
BstMAI GTCTC 4 cut(s) 390, 431, 741, 797
BstMBI GATC 7 cut(s) 100, 475, 535, 790, 968, 1320, 1351
BstMWI GCNNNNNNNGC 4 cut(s) 527, 668, 944, 953
BstNI CCWGG 3 cut(s) 176, 288, 766
BstNSI RCATGY 1 cut(s) 1309
BstSCI CCNGG 4 cut(s) 174, 286, 764, 1316
BstSFI CTRYAG 2 cut(s) 486, 666
BstSLI GKGCMC 1 cut(s) 133
BstUI CGCG 2 cut(s) 996, 1291
BstV1I GCAGC 8 cut(s) 370, 391, 472, 553, 683, 1013, 1087, 1241
BstV2I GAAGAC 1 cut(s) 235
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
BsuI GTATCC 1 cut(s) 17
BsuRI GGCC 1 cut(s) 1186
BtgI CCRYGG 1 cut(s) 328
BtgZI GCGATG 1 cut(s) 1011
BtsCI GGATG 2 cut(s) 507, 1213
BtsI GCAGTG 1 cut(s) 1350
BtsIMutI CAGTG 3 cut(s) 96, 471, 1350
Cac8I GCNNGC 3 cut(s) 564, 992, 1293
CaiI CAGNNNCTG 2 cut(s) 895, 1349
CciI TCATGA 1 cut(s) 1051
CfoI GCGC 1 cut(s) 996
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 2 cut(s) 443, 1149
CseI GACGC 2 cut(s) 527, 791
CsiI ACCWGGT 1 cut(s) 286
Csp6I GTAC 6 cut(s) 23, 54, 93, 735, 898, 1285
CviQI GTAC 6 cut(s) 23, 54, 93, 735, 898, 1285
DdeI CTNAG 3 cut(s) 89, 1162, 1365
DpnI GATC 7 cut(s) 102, 477, 537, 792, 970, 1322, 1353
DpnII GATC 7 cut(s) 100, 475, 535, 790, 968, 1320, 1351
DriI GACNNNNNGTC 1 cut(s) 290
Eam1105I GACNNNNNGTC 1 cut(s) 290
EciI GGCGGA 1 cut(s) 231
Ecl136II GAGCTC 1 cut(s) 1037
Eco130I CCWWGG 4 cut(s) 10, 70, 328, 339
Eco147I AGGCCT 1 cut(s) 1186
Eco24I GRGCYC 2 cut(s) 1039, 1163
Eco47I GGWCC 2 cut(s) 443, 1149
Eco53kI GAGCTC 1 cut(s) 1037
Eco57I CTGAAG 1 cut(s) 1173
EcoICRI GAGCTC 1 cut(s) 1037
EcoRII CCWGG 3 cut(s) 174, 286, 764
EcoT14I CCWWGG 4 cut(s) 10, 70, 328, 339
EcoT22I ATGCAT 1 cut(s) 196
EcoT38I GRGCYC 2 cut(s) 1039, 1163
ErhI CCWWGG 4 cut(s) 10, 70, 328, 339
FaqI GGGAC 1 cut(s) 1162
FauNDI CATATG 1 cut(s) 1273
FbaI TGATCA 3 cut(s) 100, 475, 1351
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 8 cut(s) 384, 405, 486, 567, 672, 1002, 1076, 1255
FokI GGATG 2 cut(s) 514, 1220
FriOI GRGCYC 2 cut(s) 1039, 1163
Fsp4HI GCNGC 8 cut(s) 384, 405, 486, 567, 672, 1002, 1076, 1255
FspBI CTAG 5 cut(s) 282, 507, 599, 1202, 1328
GlaI GCGC 1 cut(s) 995
GluI GCNGC 8 cut(s) 384, 405, 486, 567, 672, 1002, 1076, 1255
GsuI CTGGAG 2 cut(s) 455, 732
HaeIII GGCC 1 cut(s) 1186
HapII CCGG 2 cut(s) 144, 1317
HgaI GACGC 2 cut(s) 527, 791
HhaI GCGC 1 cut(s) 996
Hin1I GRCGYC 1 cut(s) 321
Hin6I GCGC 1 cut(s) 994
HinP1I GCGC 1 cut(s) 994
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HindIII AAGCTT 2 cut(s) 603, 1356
HinfI GANTC 4 cut(s) 219, 611, 848, 1249
HpaI GTTAAC 1 cut(s) 364
HpaII CCGG 2 cut(s) 144, 1317
Hpy166II GTNNAC 6 cut(s) 17, 325, 364, 900, 1030, 1287
Hpy188I TCNGA 4 cut(s) 201, 834, 1165, 1351
Hpy188III TCNNGA 9 cut(s) 208, 434, 446, 711, 722, 966, 1052, 1191, 1225
Hpy8I GTNNAC 6 cut(s) 17, 325, 364, 900, 1030, 1287
Hpy99I CGWCG 1 cut(s) 785
HpyAV CCTTC 5 cut(s) 972, 1092, 1150, 1171, 1197
HpyCH4III ACNGT 1 cut(s) 707
HpyCH4IV ACGT 2 cut(s) 321, 1105
HpyCH4V TGCA 9 cut(s) 194, 407, 488, 698, 796, 862, 1001, 1311, 1343
HpyF10VI GCNNNNNNNGC 4 cut(s) 527, 668, 944, 953
HpyF3I CTNAG 3 cut(s) 89, 1162, 1365
HpySE526I ACGT 2 cut(s) 321, 1105
Hsp92I GRCGYC 1 cut(s) 321
HspAI GCGC 1 cut(s) 994
Ksp22I TGATCA 3 cut(s) 100, 475, 1351
KspAI GTTAAC 1 cut(s) 364
Kzo9I GATC 7 cut(s) 100, 475, 535, 790, 968, 1320, 1351
LmnI GCTCC 2 cut(s) 943, 1042
Lsp1109I GCAGC 8 cut(s) 370, 391, 472, 553, 683, 1013, 1087, 1241
LweI GCATC 3 cut(s) 346, 492, 988
MabI ACCWGGT 1 cut(s) 286
MaeI CTAG 5 cut(s) 282, 507, 599, 1202, 1328
MaeII ACGT 2 cut(s) 321, 1105
MaeIII GTNAC 3 cut(s) 155, 509, 878
MalI GATC 7 cut(s) 102, 477, 537, 792, 970, 1322, 1353
MboI GATC 7 cut(s) 100, 475, 535, 790, 968, 1320, 1351
MboII GAAGA 1 cut(s) 235
MflI RGATCY 1 cut(s) 535
MhlI GDGCHC 3 cut(s) 133, 1039, 1163
MluCI AATT 4 cut(s) 138, 373, 1089, 1166
MlyI GAGTC 3 cut(s) 213, 620, 1258
MmeI TCCRAC 2 cut(s) 750, 1064
Mph1103I ATGCAT 1 cut(s) 196
MroXI GAANNNNTTC 1 cut(s) 828
MseI TTAA 2 cut(s) 300, 363
MslI CAYNNNNRTG 2 cut(s) 905, 933
MspI CCGG 2 cut(s) 144, 1317
MspR9I CCNGG 4 cut(s) 176, 288, 766, 1318
MvaI CCWGG 3 cut(s) 176, 288, 766
MvnI CGCG 2 cut(s) 996, 1291
MwoI GCNNNNNNNGC 4 cut(s) 527, 668, 944, 953
NciI CCSGG 1 cut(s) 1318
NcoI CCATGG 1 cut(s) 328
NdeI CATATG 1 cut(s) 1273
NdeII GATC 7 cut(s) 100, 475, 535, 790, 968, 1320, 1351
NlaIV GGNNCC 1 cut(s) 1150
NmuCI GTSAC 1 cut(s) 509
NsiI ATGCAT 1 cut(s) 196
NspI RCATGY 1 cut(s) 1309
PagI TCATGA 1 cut(s) 1051
PceI AGGCCT 1 cut(s) 1186
PciI ACATGT 1 cut(s) 1305
PdmI GAANNNNTTC 1 cut(s) 828
PfeI GAWTC 1 cut(s) 848
PkrI GCNGC 8 cut(s) 385, 406, 487, 568, 673, 1003, 1077, 1256
PleI GAGTC 3 cut(s) 213, 619, 1257
PpsI GAGTC 3 cut(s) 213, 619, 1257
PscI ACATGT 1 cut(s) 1305
Psp124BI GAGCTC 1 cut(s) 1039
Psp6I CCWGG 3 cut(s) 174, 286, 764
PspGI CCWGG 3 cut(s) 174, 286, 764
PspN4I GGNNCC 1 cut(s) 1150
PspPI GGNCC 2 cut(s) 443, 1149
PstI CTGCAG 1 cut(s) 490
PstNI CAGNNNCTG 2 cut(s) 895, 1349
PsuI RGATCY 1 cut(s) 535
RsaI GTAC 6 cut(s) 24, 55, 94, 736, 899, 1286
RsaNI GTAC 6 cut(s) 23, 54, 93, 735, 898, 1285
RseI CAYNNNNRTG 2 cut(s) 905, 933
SacI GAGCTC 1 cut(s) 1039
SaqAI TTAA 2 cut(s) 300, 363
SatI GCNGC 8 cut(s) 384, 405, 486, 567, 672, 1002, 1076, 1255
Sau3AI GATC 7 cut(s) 100, 475, 535, 790, 968, 1320, 1351
Sau96I GGNCC 2 cut(s) 443, 1149
ScaI AGTACT 1 cut(s) 55
SchI GAGTC 3 cut(s) 213, 620, 1258
ScrFI CCNGG 4 cut(s) 176, 288, 766, 1318
SduI GDGCHC 3 cut(s) 133, 1039, 1163
SexAI ACCWGGT 1 cut(s) 286
SfaNI GCATC 3 cut(s) 346, 492, 988
SfcI CTRYAG 2 cut(s) 486, 666
SinI GGWCC 2 cut(s) 443, 1149
SmiMI CAYNNNNRTG 2 cut(s) 905, 933
SmlI CTYRAG 2 cut(s) 206, 976
SmoI CTYRAG 2 cut(s) 206, 976
Sse9I AATT 4 cut(s) 138, 373, 1089, 1166
SseBI AGGCCT 1 cut(s) 1186
SsiI CCGC 2 cut(s) 216, 1257
SspMI CTAG 5 cut(s) 282, 507, 599, 1202, 1328
SstI GAGCTC 1 cut(s) 1039
StuI AGGCCT 1 cut(s) 1186
StyD4I CCNGG 4 cut(s) 174, 286, 764, 1316
StyI CCWWGG 4 cut(s) 10, 70, 328, 339
TaaI ACNGT 1 cut(s) 707
TaiI ACGT 2 cut(s) 324, 1108
TaqI TCGA 5 cut(s) 113, 149, 169, 1062, 1268
TaqII GACCGA 1 cut(s) 1166
TasI AATT 4 cut(s) 138, 373, 1089, 1166
TatI WGTACW 3 cut(s) 53, 897, 1284
TfiI GAWTC 1 cut(s) 848
Tru1I TTAA 2 cut(s) 300, 363
Tru9I TTAA 2 cut(s) 300, 363
TscAI CASTG 3 cut(s) 103, 478, 1350
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 8 cut(s) 383, 404, 485, 566, 671, 1001, 1075, 1254
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 8 cut(s) 235, 243, 451, 837, 861, 1101, 1190, 1295
TspRI CASTG 3 cut(s) 103, 478, 1350
VpaK11BI GGWCC 2 cut(s) 443, 1149
XapI RAATTY 1 cut(s) 373
XceI RCATGY 1 cut(s) 1309
XmiI GTMKAC 1 cut(s) 324
XmnI GAANNNNTTC 1 cut(s) 828
XspI CTAG 5 cut(s) 282, 507, 599, 1202, 1328
ZraI GACGTC 1 cut(s) 322
ZrmI AGTACT 1 cut(s) 55
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.