Rmu_sc0002283.1_g000057

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002283.1
OG0001085
Physical Location & Seq
Forward (+)
223394 .. 227932
4539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002283.1_g000057.1.cds

Sequence Viewer

Length: 1587 bp
atgaaagggcagtactgttccagtggagtatttttggtattgcttaccatctttggtgtgagcggtgctcaacttcccaagttgatgccacaagaatttccagaggatttcattattcatgttgataggggctctagcaacagtgatacaaatggaacaagatgggcagttgtagttgctgggtctatgggttacggaaactataggcatcaggctgatgtgtgtcatgcatatcaaattttgaagaaaggtggactgaaagatgaaaacatcattgttttcatgtacgatgacattgcaaacaaccctgagaatccaaggccgggtgtcatcatcaacaatcccaacggcgatgacgtctaccatggagttcccaaggattacacaggagagaatgctactgctgctaatctttatgctgtcattcttggagataaatctaatctctctgggggtagtggcaaggttctgaacagcgggccagacgacaatgtctttatctattacaccgaccatggcgggcctgggatcattgggatgcctgaaggcgattatgtctatgccaatgatctggtagatgttctgataaagaagtatgaagctaatgcgtacaaaaacatggtattttaccttgaagcttgtgaatccgggagtatgttcgagggtctactgccgacaaatataaacatctatgcaaccacagcatcaaatgcaactgagagtagttggggaacgtactgccctgatatgactcctcctccacctcctgaatatgacacttgcttgggtgatttgtatagtgtttcttggatggagaattgtgacttatcagatttgcgccaagagactctggaagagcaatatcaatcggttaaatggagaacagtcaataatacttatggttctcatgttatgcaatatggaaatatgtatcaaacctcagatttagtatccttttacttgggtgcaaatgataactcttcctccattgaaagcaaatcgccacagtcaatcttagaagttgttgacaatcgcgatgcagatcttcttcatctttggcacaagttccaacgcgcacccgctggctctgaaatgaagcaggaagctaaaagggtcctagatgatgaaatttcaagtaggcagcgcattgaccacaacataaaccaaatcgaacaacttgtgttgggctattcaagtaactctgttcgtcctgggggacaagctcttgttgatgattgggactgcttcaagacagttgtgaaaacttatgagaaacattgtgggtcattgtcaaattatgcctacacgggcctcacctgcgcagcccagccctgccaccaaacctcaccggtcagaccggcctcacctcaccaaccccgccttcgcctccgccttgcacgtccgacatttgcagtcaccgacctcacctcaccaacccacctgcaacggcgctgctttctcgcccagcccagatttgcttgcgtccttcgcccagatttgcagcagcaccacaaacctgaagcgcagcctcgcctagcctcgagtggcctcccacctcgcctagccacctcagcccaccatctgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

528

Amino Acids

58.58

Weight (kDa)

5.49

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1325, 1449
AasI GACNNNNNNGTC 1 cut(s) 491
AatII GACGTC 1 cut(s) 360
Acc16I TGCGCA 1 cut(s) 1321
Acc36I ACCTGC 2 cut(s) 1325, 1449
AccBSI CCGCTC 1 cut(s) 63
AccI GTMKAC 2 cut(s) 360, 667
AccII CGCG 2 cut(s) 1035, 1074
AciI CCGC 6 cut(s) 63, 477, 519, 1080, 1378, 1390
AclWI GGATC 1 cut(s) 536
AcsI RAATTY 3 cut(s) 95, 237, 1128
AcuI CTGAAG 2 cut(s) 564, 1539
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 4 cut(s) 14, 287, 611, 737
AfiI CCNNNNNNNGG 1 cut(s) 323
AgeI ACCGGT 1 cut(s) 1348
AgsI TTSAA 6 cut(s) 244, 635, 992, 1134, 1194, 1249
AjiI CACGTC 1 cut(s) 1400
AjnI CCWGG 2 cut(s) 523, 1210
AjuI GAANNNNNNNTTGG 2 cut(s) 1365, 1397
AluBI AGCT 4 cut(s) 602, 638, 1106, 1223
AluI AGCT 4 cut(s) 602, 638, 1106, 1223
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 1 cut(s) 839
AlwI GGATC 1 cut(s) 536
Ama87I CYCGRG 1 cut(s) 1540
AoxI GGCC 6 cut(s) 320, 479, 521, 1309, 1359, 1546
ApeKI GCWGC 7 cut(s) 404, 1141, 1322, 1452, 1501, 1504, 1525
ApoI RAATTY 3 cut(s) 95, 237, 1128
AsiGI ACCGGT 1 cut(s) 1348
AspLEI GCGC 6 cut(s) 840, 1076, 1146, 1322, 1452, 1525
AspS9I GGNCC 4 cut(s) 479, 521, 1114, 1309
AsuC2I CCSGG 2 cut(s) 324, 649
AsuHPI GGTGA 8 cut(s) 800, 1306, 1338, 1356, 1361, 1408, 1417, 1422
AvaI CYCGRG 1 cut(s) 1540
AvaII GGWCC 1 cut(s) 1114
BanII GRGCYC 1 cut(s) 134
Bbv12I GWGCWC 1 cut(s) 70
BbvCI CCTCAGC 1 cut(s) 1570
BbvI GCAGC 7 cut(s) 391, 1153, 1334, 1439, 1513, 1516, 1537
BccI CCATC 3 cut(s) 56, 156, 805
BceAI ACGGC 2 cut(s) 364, 1463
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 2 cut(s) 525, 1212
BciVI GTATCC 1 cut(s) 961
BcnI CCSGG 2 cut(s) 324, 649
BcoDI GTCTC 1 cut(s) 839
BfaI CTAG 4 cut(s) 135, 1118, 1535, 1562
BfmI CTRYAG 1 cut(s) 202
BfoI RGCGCY 1 cut(s) 1453
BfuAI ACCTGC 2 cut(s) 1325, 1449
BfuI GTATCC 1 cut(s) 961
BglII AGATCT 1 cut(s) 1042
BisI GCNGC 7 cut(s) 405, 1142, 1323, 1453, 1502, 1505, 1526
BlsI GCNGC 7 cut(s) 406, 1143, 1324, 1454, 1503, 1506, 1527
BmcAI AGTACT 1 cut(s) 14
Bme1390I CCNGG 4 cut(s) 324, 525, 649, 1212
Bme18I GGWCC 1 cut(s) 1114
BmeT110I CYCGRG 1 cut(s) 1540
BmgBI CACGTC 1 cut(s) 1400
BmgT120I GGNCC 4 cut(s) 479, 521, 1114, 1309
BmiI GGNNCC 1 cut(s) 1115
BmrFI CCNGG 4 cut(s) 324, 525, 649, 1212
BmsI GCATC 5 cut(s) 75, 217, 528, 713, 1027
BplI GAGNNNNNCTC 2 cut(s) 52, 84
Bpu10I CCTNAGC 1 cut(s) 1570
BpuMI CCSGG 2 cut(s) 324, 649
BsaBI GATNNNNATC 1 cut(s) 1041
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 6 cut(s) 317, 364, 375, 514, 524, 1211
BsaWI WCCGGW 1 cut(s) 1348
BsaXI ACNNNNNCTCC 4 cut(s) 742, 772, 968, 998
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse118I RCCGGY 2 cut(s) 1348, 1357
Bse1I ACTGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 294
Bse8I GATNNNNATC 1 cut(s) 1041
BseBI CCWGG 2 cut(s) 525, 1212
BseDI CCNNGG 6 cut(s) 317, 364, 375, 514, 524, 1211
BseGI GGATG 2 cut(s) 543, 816
BseJI GATNNNNATC 1 cut(s) 1041
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 4 cut(s) 300, 708, 954, 1584
BseNI ACTGG 1 cut(s) 21
BseRI GAGGAG 2 cut(s) 744, 747
BseXI GCAGC 7 cut(s) 391, 1153, 1334, 1439, 1513, 1516, 1537
BseYI CCCAGC 3 cut(s) 179, 1326, 1464
Bsh1236I CGCG 2 cut(s) 1035, 1074
BshFI GGCC 6 cut(s) 322, 481, 523, 1311, 1361, 1548
BshTI ACCGGT 1 cut(s) 1348
BsiHKAI GWGCWC 1 cut(s) 70
BsiHKCI CYCGRG 1 cut(s) 1540
BsiSI CCGG 4 cut(s) 323, 648, 1349, 1358
BslFI GGGAC 2 cut(s) 1230, 1253
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 1 cut(s) 839
BsmFI GGGAC 2 cut(s) 1230, 1253
BsmI GAATGC 1 cut(s) 400
BsnI GGCC 6 cut(s) 322, 481, 523, 1311, 1361, 1548
BsoBI CYCGRG 1 cut(s) 1540
Bsp1286I GDGCHC 2 cut(s) 70, 134
Bsp143I GATC 3 cut(s) 528, 568, 1042
Bsp19I CCATGG 2 cut(s) 364, 514
Bsp68I TCGCGA 1 cut(s) 1035
BspACI CCGC 6 cut(s) 63, 477, 519, 1080, 1378, 1390
BspANI GGCC 6 cut(s) 322, 481, 523, 1311, 1361, 1548
BspCNI CTCAG 4 cut(s) 301, 709, 953, 1583
BspFNI CGCG 2 cut(s) 1035, 1074
BspLI GGNNCC 1 cut(s) 1115
BspMI ACCTGC 2 cut(s) 1325, 1449
BspPI GGATC 1 cut(s) 536
BspQI GCTCTTC 1 cut(s) 849
BsrBI CCGCTC 1 cut(s) 63
BsrDI GCAATG 1 cut(s) 294
BsrFI RCCGGY 2 cut(s) 1348, 1357
BsrI ACTGG 1 cut(s) 21
BssAI RCCGGY 2 cut(s) 1348, 1357
BssECI CCNNGG 6 cut(s) 317, 364, 375, 514, 524, 1211
BssMI GATC 3 cut(s) 528, 568, 1042
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 4 cut(s) 317, 364, 375, 514
Bst2UI CCWGG 2 cut(s) 525, 1212
Bst4CI ACNGT 5 cut(s) 17, 143, 886, 1008, 1255
Bst6I CTCTTC 2 cut(s) 849, 985
BstACI GRCGYC 1 cut(s) 357
BstAPI GCANNNNNTGC 1 cut(s) 710
BstC8I GCNNGC 4 cut(s) 479, 521, 1084, 1480
BstDEI CTNAG 5 cut(s) 309, 717, 940, 1015, 1570
BstDSI CCRYGG 2 cut(s) 364, 514
BstF5I GGATG 2 cut(s) 543, 816
BstFNI CGCG 2 cut(s) 1035, 1074
BstH2I RGCGCY 1 cut(s) 1453
BstHHI GCGC 6 cut(s) 840, 1076, 1146, 1322, 1452, 1525
BstKTI GATC 3 cut(s) 531, 571, 1045
BstMAI GTCTC 1 cut(s) 839
BstMBI GATC 3 cut(s) 528, 568, 1042
BstMWI GCNNNNNNNGC 6 cut(s) 404, 701, 710, 1317, 1488, 1571
BstNI CCWGG 2 cut(s) 525, 1212
BstSCI CCNGG 4 cut(s) 322, 523, 647, 1210
BstSFI CTRYAG 1 cut(s) 202
BstUI CGCG 2 cut(s) 1035, 1074
BstV1I GCAGC 7 cut(s) 391, 1153, 1334, 1439, 1513, 1516, 1537
BstX2I RGATCY 1 cut(s) 1042
BstXI CCANNNNNNTGG 1 cut(s) 571
BstYI RGATCY 1 cut(s) 1042
BsuI GTATCC 1 cut(s) 961
BsuRI GGCC 6 cut(s) 322, 481, 523, 1311, 1361, 1548
BtgI CCRYGG 2 cut(s) 364, 514
BtgZI GCGATG 2 cut(s) 366, 1050
BtrI CACGTC 1 cut(s) 1400
BtsCI GGATG 2 cut(s) 543, 816
BtsIMutI CAGTG 2 cut(s) 28, 148
BtuMI TCGCGA 1 cut(s) 1035
BveI ACCTGC 2 cut(s) 1325, 1449
Cac8I GCNNGC 4 cut(s) 479, 521, 1084, 1480
CfoI GCGC 6 cut(s) 840, 1076, 1146, 1322, 1452, 1525
Cfr10I RCCGGY 2 cut(s) 1348, 1357
Cfr13I GGNCC 4 cut(s) 479, 521, 1114, 1309
CseI GACGC 1 cut(s) 1471
Csp6I GTAC 4 cut(s) 13, 286, 610, 736
CspAI ACCGGT 1 cut(s) 1348
CviAII CATG 7 cut(s) 119, 227, 283, 365, 515, 619, 908
CviQI GTAC 4 cut(s) 13, 286, 610, 736
DdeI CTNAG 5 cut(s) 309, 717, 940, 1015, 1570
DpnI GATC 3 cut(s) 530, 570, 1044
DpnII GATC 3 cut(s) 528, 568, 1042
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
Eam1104I CTCTTC 2 cut(s) 849, 985
EarI CTCTTC 2 cut(s) 849, 985
EciI GGCGGA 1 cut(s) 1379
Eco130I CCWWGG 4 cut(s) 317, 364, 375, 514
Eco24I GRGCYC 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 1114
Eco57I CTGAAG 2 cut(s) 564, 1539
Eco88I CYCGRG 1 cut(s) 1540
EcoO109I RGGNCCY 1 cut(s) 1114
EcoRII CCWGG 2 cut(s) 523, 1210
EcoT14I CCWWGG 4 cut(s) 317, 364, 375, 514
EcoT22I ATGCAT 1 cut(s) 232
EcoT38I GRGCYC 1 cut(s) 134
ErhI CCWWGG 4 cut(s) 317, 364, 375, 514
FaeI CATG 7 cut(s) 122, 230, 286, 368, 518, 622, 911
FaqI GGGAC 2 cut(s) 1230, 1253
FatI CATG 7 cut(s) 118, 226, 282, 364, 514, 618, 907
FauI CCCGC 4 cut(s) 470, 512, 1087, 1385
FblI GTMKAC 2 cut(s) 360, 667
Fnu4HI GCNGC 7 cut(s) 405, 1142, 1323, 1453, 1502, 1505, 1526
FokI GGATG 2 cut(s) 550, 823
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 7 cut(s) 405, 1142, 1323, 1453, 1502, 1505, 1526
FspBI CTAG 4 cut(s) 135, 1118, 1535, 1562
FspI TGCGCA 1 cut(s) 1321
GlaI GCGC 6 cut(s) 839, 1075, 1145, 1321, 1451, 1524
GluI GCNGC 7 cut(s) 405, 1142, 1323, 1453, 1502, 1505, 1526
GsaI CCCAGC 3 cut(s) 183, 1330, 1468
HaeII RGCGCY 1 cut(s) 1453
HaeIII GGCC 6 cut(s) 322, 481, 523, 1311, 1361, 1548
HapII CCGG 4 cut(s) 323, 648, 1349, 1358
HgaI GACGC 1 cut(s) 1471
HhaI GCGC 6 cut(s) 840, 1076, 1146, 1322, 1452, 1525
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 7 cut(s) 122, 230, 286, 368, 518, 622, 911
Hin6I GCGC 6 cut(s) 838, 1074, 1144, 1320, 1450, 1523
HinP1I GCGC 6 cut(s) 838, 1074, 1144, 1320, 1450, 1523
HincII GTYRAC 1 cut(s) 1027
HindII GTYRAC 1 cut(s) 1027
HindIII AAGCTT 1 cut(s) 636
HinfI GANTC 4 cut(s) 313, 644, 751, 847
HpaII CCGG 4 cut(s) 323, 648, 1349, 1358
HphI GGTGA 8 cut(s) 800, 1306, 1338, 1356, 1361, 1408, 1417, 1422
Hpy166II GTNNAC 4 cut(s) 254, 361, 668, 1027
Hpy188I TCNGA 7 cut(s) 471, 585, 832, 943, 1090, 1355, 1404
Hpy188III TCNNGA 5 cut(s) 101, 767, 851, 1034, 1249
Hpy8I GTNNAC 4 cut(s) 254, 361, 668, 1027
HpyAV CCTTC 3 cut(s) 539, 1391, 1496
HpyCH4III ACNGT 5 cut(s) 17, 143, 886, 1008, 1255
HpyCH4IV ACGT 3 cut(s) 357, 734, 1399
HpyF10VI GCNNNNNNNGC 6 cut(s) 404, 701, 710, 1317, 1488, 1571
HpyF3I CTNAG 5 cut(s) 309, 717, 940, 1015, 1570
HpySE526I ACGT 3 cut(s) 357, 734, 1399
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 7 cut(s) 122, 230, 286, 368, 518, 622, 911
HspAI GCGC 6 cut(s) 838, 1074, 1144, 1320, 1450, 1523
Kzo9I GATC 3 cut(s) 528, 568, 1042
LguI GCTCTTC 1 cut(s) 849
Lsp1109I GCAGC 7 cut(s) 391, 1153, 1334, 1439, 1513, 1516, 1537
LweI GCATC 5 cut(s) 75, 217, 528, 713, 1027
MaeI CTAG 4 cut(s) 135, 1118, 1535, 1562
MaeII ACGT 3 cut(s) 357, 734, 1399
MaeIII GTNAC 4 cut(s) 191, 821, 1196, 1414
MalI GATC 3 cut(s) 530, 570, 1044
MbiI CCGCTC 1 cut(s) 63
MboI GATC 3 cut(s) 528, 568, 1042
MboII GAAGA 5 cut(s) 256, 866, 972, 1037, 1040
MflI RGATCY 1 cut(s) 1042
MhlI GDGCHC 2 cut(s) 70, 134
MluCI AATT 5 cut(s) 95, 237, 817, 1128, 1294
MlyI GAGTC 2 cut(s) 745, 841
MmeI TCCRAC 2 cut(s) 1093, 1427
Mph1103I ATGCAT 1 cut(s) 232
MseI TTAA 1 cut(s) 873
MslI CAYNNNNRTG 1 cut(s) 536
MspA1I CMGCKG 2 cut(s) 477, 1082
MspI CCGG 4 cut(s) 323, 648, 1349, 1358
MspR9I CCNGG 4 cut(s) 324, 525, 649, 1212
Mva1269I GAATGC 1 cut(s) 400
MvaI CCWGG 2 cut(s) 525, 1212
MvnI CGCG 2 cut(s) 1035, 1074
MwoI GCNNNNNNNGC 6 cut(s) 404, 701, 710, 1317, 1488, 1571
NciI CCSGG 2 cut(s) 324, 649
NcoI CCATGG 2 cut(s) 364, 514
NdeII GATC 3 cut(s) 528, 568, 1042
NlaIII CATG 7 cut(s) 122, 230, 286, 368, 518, 622, 911
NlaIV GGNNCC 1 cut(s) 1115
NmuCI GTSAC 2 cut(s) 821, 1414
NruI TCGCGA 1 cut(s) 1035
NsbI TGCGCA 1 cut(s) 1321
NsiI ATGCAT 1 cut(s) 232
PaeR7I CTCGAG 1 cut(s) 1540
PaqCI CACCTGC 2 cut(s) 1325, 1449
PciSI GCTCTTC 1 cut(s) 849
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PctI GAATGC 1 cut(s) 400
PfeI GAWTC 2 cut(s) 313, 644
PflFI GACNNNGTC 1 cut(s) 491
PfoI TCCNGGA 1 cut(s) 647
PinAI ACCGGT 1 cut(s) 1348
PkrI GCNGC 7 cut(s) 406, 1143, 1324, 1454, 1503, 1506, 1527
PleI GAGTC 2 cut(s) 745, 841
PpsI GAGTC 2 cut(s) 745, 841
PpuMI RGGWCCY 1 cut(s) 1114
Psp5II RGGWCCY 1 cut(s) 1114
Psp6I CCWGG 2 cut(s) 523, 1210
PspFI CCCAGC 3 cut(s) 179, 1326, 1464
PspGI CCWGG 2 cut(s) 523, 1210
PspN4I GGNNCC 1 cut(s) 1115
PspPI GGNCC 4 cut(s) 479, 521, 1114, 1309
PspPPI RGGWCCY 1 cut(s) 1114
PspXI VCTCGAGB 1 cut(s) 1540
PsuI RGATCY 1 cut(s) 1042
PsyI GACNNNGTC 1 cut(s) 491
RruI TCGCGA 1 cut(s) 1035
RsaI GTAC 4 cut(s) 14, 287, 611, 737
RsaNI GTAC 4 cut(s) 13, 286, 610, 736
RseI CAYNNNNRTG 1 cut(s) 536
SapI GCTCTTC 1 cut(s) 849
SaqAI TTAA 1 cut(s) 873
SatI GCNGC 7 cut(s) 405, 1142, 1323, 1453, 1502, 1505, 1526
Sau3AI GATC 3 cut(s) 528, 568, 1042
Sau96I GGNCC 4 cut(s) 479, 521, 1114, 1309
ScaI AGTACT 1 cut(s) 14
SchI GAGTC 2 cut(s) 745, 841
ScrFI CCNGG 4 cut(s) 324, 525, 649, 1212
SduI GDGCHC 2 cut(s) 70, 134
SfaNI GCATC 5 cut(s) 75, 217, 528, 713, 1027
SfcI CTRYAG 1 cut(s) 202
Sfr274I CTCGAG 1 cut(s) 1540
SinI GGWCC 1 cut(s) 1114
SlaI CTCGAG 1 cut(s) 1540
SmiMI CAYNNNNRTG 1 cut(s) 536
SmlI CTYRAG 1 cut(s) 1540
SmoI CTYRAG 1 cut(s) 1540
Sse9I AATT 5 cut(s) 95, 237, 817, 1128, 1294
SsiI CCGC 6 cut(s) 63, 477, 519, 1080, 1378, 1390
SspMI CTAG 4 cut(s) 135, 1118, 1535, 1562
StyD4I CCNGG 4 cut(s) 322, 523, 647, 1210
StyI CCWWGG 4 cut(s) 317, 364, 375, 514
TaaI ACNGT 5 cut(s) 17, 143, 886, 1008, 1255
TaiI ACGT 3 cut(s) 360, 737, 1402
TaqI TCGA 3 cut(s) 660, 1170, 1541
TasI AATT 5 cut(s) 95, 237, 817, 1128, 1294
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 2 cut(s) 313, 644
Tru1I TTAA 1 cut(s) 873
Tru9I TTAA 1 cut(s) 873
TscAI CASTG 2 cut(s) 28, 148
TseFI GTSAC 2 cut(s) 821, 1414
TseI GCWGC 7 cut(s) 404, 1141, 1322, 1452, 1501, 1504, 1525
Tsp45I GTSAC 2 cut(s) 821, 1414
TspDTI ATGAA 9 cut(s) 17, 100, 107, 271, 279, 612, 1040, 1109, 1140
TspGWI ACGGA 1 cut(s) 210
TspRI CASTG 2 cut(s) 28, 148
Tth111I GACNNNGTC 1 cut(s) 491
VpaK11BI GGWCC 1 cut(s) 1114
XapI RAATTY 3 cut(s) 95, 237, 1128
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XhoI CTCGAG 1 cut(s) 1540
XmiI GTMKAC 2 cut(s) 360, 667
XspI CTAG 4 cut(s) 135, 1118, 1535, 1562
ZraI GACGTC 1 cut(s) 358
ZrmI AGTACT 1 cut(s) 14
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.