Prupe.7G024000_v2.0.a1

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001085
Physical Location & Seq
Reverse (-)
3797749 .. 3801102
3354 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G024000.1

Sequence Viewer

Length: 1290 bp
GGCTCATCATCATCTACTGCGACTGAGCAAAATGGAACCAGATGGGCAGTCCTAGTTGCTGGTTCATATGGTTATCAGAACTATAGGCATCAGGCGGATGTATGTCATGCATATCAAATATTGAAGAAAGGCGGATTGAAAGATGAAAACATTATTGTTTTTATGTATGATGACATAGCATTCAATGTTCAGAACCCTAGACCTGGTGTCATCATCAACAAGCCAGATGGTCCAGATGTTTACGAAGGAGTTCCCAAGGATTATACAGGAACCAATGTGACCGCTGCCAATCTTTATGCCGTTATTCTTGGAAATAAAACTGCTCTCACCGGGGGCAGTGGCAAGGTTCTAGACAGTGGTCCAAATGACAACATTTTTATTTACTATACAGACCATGGTGCCGCTGGAATAATCGGGATGCCTGAAGGCGACTATGTTTATGCAAACGATCTAGTGGATGTTCTGAAACAGAAGCATGAAGCCAAGTCCTACAAAGATATGGTATTTTACCTTGAATCTTGTGAATCTGGGAGCATGTTCGAGGGCCTCCTTCCAAACAATATAAGTATCTATGCGACCACAGCAGCAAATGCAACGGAAAACAGTTGGGGAACATACTGTCCCGGTTTTAATCCTGGTCCTCCTGCAGGATATACCACTTGTTTGGGAGACTTGTATAGTATTTCTTGGATGGAGGATTGTGACATCAAAGATTTGCGGAGAGAAACTTTTGAACAGCAATATGAAGCAGTTAGAAAGAGAACAAGAAATACTACTGAGGGACTAGGTTCTCATGTTACTCAGTATGGAAATATGAATCAAGCCAACGATTTACTCTTCTCTTACATGGGTTCAAATGATAACTCTACTTCTTACACCCAAAGTGAATCGTCCCCATCGCTCTTAGGATCCATTTTCAACCAACGTGATGCAGATCTCCTTCACTTTTTGGTCAAGTCGAGTAAAGCTCCGATTGGGTCTGATGAGAGGCATGAAGCTAGAGAGGAACTACGTGCTGAGGTTGATCGCAGGAAGCGCATCGACGACAATGTAGATCAAATCCGGTGGCGTTTGTTTGGTCGTGAGAGTGACTTGAAGAATATTCGACCTCAGAGTCAAAGCCTTGTAGATGATTGGAGCTGCTTCAAGATGCTTGTGAGAACTTATGAGAAATATTGTGGGTCACTCTCGGATTATGGAATGCAATACACACGAGTTTTTGCCAACATGTGCAATGCTGGGGTTACCAAAGAGCGAATGATTGCAGCATCAACTCAAACGTGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

430

Amino Acids

47.82

Weight (kDa)

5.17

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1113
AccB1I GGYRCC 1 cut(s) 398
AciI CCGC 5 cut(s) 95, 132, 282, 402, 718
AclWI GGATC 2 cut(s) 903, 916
AcuI CTGAAG 1 cut(s) 444
AfiI CCNNNNNNNGG 2 cut(s) 203, 647
AflIII ACRYGT 2 cut(s) 1227, 1280
AgsI TTSAA 9 cut(s) 124, 139, 184, 515, 734, 855, 919, 1096, 1147
AhdI GACNNNNNGTC 1 cut(s) 206
AjnI CCWGG 2 cut(s) 202, 634
AloI GAACNNNNNNTCC 2 cut(s) 604, 636
AluBI AGCT 3 cut(s) 968, 998, 1140
AluI AGCT 3 cut(s) 968, 998, 1140
Alw26I GTCTC 1 cut(s) 663
AlwI GGATC 2 cut(s) 903, 916
AoxI GGCC 1 cut(s) 544
ApeKI GCWGC 4 cut(s) 284, 584, 1140, 1265
Asp700I GAANNNNTTC 1 cut(s) 249
AspLEI GCGC 1 cut(s) 1038
AspS9I GGNCC 4 cut(s) 230, 359, 544, 638
AsuC2I CCSGG 2 cut(s) 331, 624
AsuHPI GGTGA 1 cut(s) 319
AvaII GGWCC 3 cut(s) 230, 359, 638
BamHI GGATCC 1 cut(s) 908
BanI GGYRCC 1 cut(s) 398
BauI CACGAG 1 cut(s) 1212
BbvCI CCTCAGC 1 cut(s) 1017
BbvI GCAGC 4 cut(s) 271, 596, 1127, 1277
BccI CCATC 4 cut(s) 36, 221, 685, 904
BceAI ACGGC 1 cut(s) 284
BciT130I CCWGG 2 cut(s) 204, 636
BcnI CCSGG 2 cut(s) 331, 624
BcoDI GTCTC 1 cut(s) 663
BfaI CTAG 6 cut(s) 53, 198, 350, 452, 785, 999
BfmI CTRYAG 2 cut(s) 82, 645
BglII AGATCT 1 cut(s) 934
BisI GCNGC 5 cut(s) 285, 402, 585, 1141, 1266
BlsI GCNGC 5 cut(s) 286, 403, 586, 1142, 1267
Bme1390I CCNGG 4 cut(s) 204, 331, 624, 636
Bme18I GGWCC 3 cut(s) 230, 359, 638
BmeRI GACNNNNNGTC 1 cut(s) 206
BmgT120I GGNCC 4 cut(s) 230, 359, 544, 638
BmiI GGNNCC 4 cut(s) 37, 271, 400, 910
BmrFI CCNGG 4 cut(s) 204, 331, 624, 636
BmsI GCATC 6 cut(s) 97, 408, 919, 1047, 1140, 1277
BoxI GACNNNNGTC 1 cut(s) 357
BplI GAGNNNNNCTC 2 cut(s) 952, 984
Bpu10I CCTNAGC 1 cut(s) 1017
BpuMI CCSGG 2 cut(s) 331, 624
BsaAI YACGTR 1 cut(s) 1013
BsaBI GATNNNNATC 1 cut(s) 933
BsaJI CCNNGG 3 cut(s) 255, 330, 394
BsaWI WCCGGW 1 cut(s) 1062
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 647
Bse3DI GCAATG 1 cut(s) 1240
Bse8I GATNNNNATC 1 cut(s) 933
BseBI CCWGG 2 cut(s) 204, 636
BseDI CCNNGG 3 cut(s) 255, 330, 394
BseGI GGATG 4 cut(s) 103, 423, 463, 696
BseJI GATNNNNATC 1 cut(s) 933
BseLI CCNNNNNNNGG 2 cut(s) 203, 647
BseMI GCAATG 1 cut(s) 1240
BseMII CTCAG 5 cut(s) 15, 768, 815, 1008, 1124
BseXI GCAGC 4 cut(s) 271, 596, 1127, 1277
BseYI CCCAGC 1 cut(s) 1238
BshFI GGCC 1 cut(s) 546
BshNI GGYRCC 1 cut(s) 398
BsiSI CCGG 3 cut(s) 330, 624, 1063
BslFI GGGAC 3 cut(s) 606, 795, 877
BslI CCNNNNNNNGG 2 cut(s) 203, 647
BsmAI GTCTC 1 cut(s) 663
BsmFI GGGAC 3 cut(s) 606, 795, 877
BsmI GAATGC 2 cut(s) 179, 1206
BsnI GGCC 1 cut(s) 546
Bsp143I GATC 5 cut(s) 448, 908, 934, 1024, 1054
Bsp19I CCATGG 1 cut(s) 394
BspACI CCGC 5 cut(s) 95, 132, 282, 402, 718
BspANI GGCC 1 cut(s) 546
BspCNI CTCAG 5 cut(s) 16, 769, 814, 1009, 1123
BspLI GGNNCC 4 cut(s) 37, 271, 400, 910
BspMAI CTGCAG 1 cut(s) 649
BspPI GGATC 2 cut(s) 903, 916
BspT107I GGYRCC 1 cut(s) 398
BsrDI GCAATG 1 cut(s) 1240
BssECI CCNNGG 3 cut(s) 255, 330, 394
BssMI GATC 5 cut(s) 448, 908, 934, 1024, 1054
BssSI CACGAG 1 cut(s) 1212
BssT1I CCWWGG 2 cut(s) 255, 394
Bst2BI CACGAG 1 cut(s) 1212
Bst2UI CCWGG 2 cut(s) 204, 636
Bst4CI ACNGT 3 cut(s) 356, 605, 620
Bst6I CTCTTC 1 cut(s) 842
BstAPI GCANNNNNTGC 1 cut(s) 590
BstBAI YACGTR 1 cut(s) 1013
BstDEI CTNAG 7 cut(s) 24, 777, 801, 904, 1017, 1110, 1287
BstDSI CCRYGG 1 cut(s) 394
BstEII GGTNACC 1 cut(s) 1243
BstENI CCTNNNNNAGG 1 cut(s) 645
BstF5I GGATG 4 cut(s) 103, 423, 463, 696
BstHHI GCGC 1 cut(s) 1038
BstKTI GATC 5 cut(s) 451, 911, 937, 1027, 1057
BstMAI GTCTC 1 cut(s) 663
BstMBI GATC 5 cut(s) 448, 908, 934, 1024, 1054
BstMWI GCNNNNNNNGC 3 cut(s) 581, 590, 1035
BstNI CCWGG 2 cut(s) 204, 636
BstNSI RCATGY 2 cut(s) 538, 1231
BstPAI GACNNNNGTC 1 cut(s) 357
BstPI GGTNACC 1 cut(s) 1243
BstSCI CCNGG 4 cut(s) 202, 329, 622, 634
BstSFI CTRYAG 2 cut(s) 82, 645
BstV1I GCAGC 4 cut(s) 271, 596, 1127, 1277
BstX2I RGATCY 2 cut(s) 908, 934
BstXI CCANNNNNNTGG 1 cut(s) 664
BstYI RGATCY 2 cut(s) 908, 934
BsuRI GGCC 1 cut(s) 546
BtgI CCRYGG 1 cut(s) 394
BtgZI GCGATG 1 cut(s) 882
BtsCI GGATG 4 cut(s) 103, 423, 463, 696
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 2 cut(s) 343, 361
CfoI GCGC 1 cut(s) 1038
Cfr13I GGNCC 4 cut(s) 230, 359, 544, 638
CsiI ACCWGGT 1 cut(s) 202
CspCI CAANNNNNGTGG 2 cut(s) 1046, 1081
CviAII CATG 8 cut(s) 107, 395, 476, 535, 794, 847, 992, 1228
CviJI RGCY 9 cut(s) 3, 223, 482, 546, 824, 968, 998, 1122, 1140
CviKI_1 RGCY 9 cut(s) 3, 223, 482, 546, 824, 968, 998, 1122, 1140
DdeI CTNAG 7 cut(s) 24, 777, 801, 904, 1017, 1110, 1287
DpnI GATC 5 cut(s) 450, 910, 936, 1026, 1056
DpnII GATC 5 cut(s) 448, 908, 934, 1024, 1054
DrdI GACNNNNNNGTC 1 cut(s) 1113
DriI GACNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 1113
Eam1104I CTCTTC 1 cut(s) 842
Eam1105I GACNNNNNGTC 1 cut(s) 206
EarI CTCTTC 1 cut(s) 842
EciI GGCGGA 2 cut(s) 110, 147
Eco130I CCWWGG 2 cut(s) 255, 394
Eco47I GGWCC 3 cut(s) 230, 359, 638
Eco57I CTGAAG 1 cut(s) 444
Eco91I GGTNACC 1 cut(s) 1243
EcoNI CCTNNNNNAGG 1 cut(s) 645
EcoO109I RGGNCCY 1 cut(s) 544
EcoO65I GGTNACC 1 cut(s) 1243
EcoRII CCWGG 2 cut(s) 202, 634
EcoT14I CCWWGG 2 cut(s) 255, 394
EcoT22I ATGCAT 1 cut(s) 112
ErhI CCWWGG 2 cut(s) 255, 394
FaeI CATG 8 cut(s) 110, 398, 479, 538, 797, 850, 995, 1231
FaqI GGGAC 3 cut(s) 606, 795, 877
FatI CATG 8 cut(s) 106, 394, 475, 534, 793, 846, 991, 1227
FauNDI CATATG 1 cut(s) 67
Fnu4HI GCNGC 5 cut(s) 285, 402, 585, 1141, 1266
FokI GGATG 4 cut(s) 110, 430, 470, 703
Fsp4HI GCNGC 5 cut(s) 285, 402, 585, 1141, 1266
FspBI CTAG 6 cut(s) 53, 198, 350, 452, 785, 999
GlaI GCGC 1 cut(s) 1037
GluI GCNGC 5 cut(s) 285, 402, 585, 1141, 1266
GsaI CCCAGC 1 cut(s) 1242
HaeIII GGCC 1 cut(s) 546
HapII CCGG 3 cut(s) 330, 624, 1063
HhaI GCGC 1 cut(s) 1038
Hin1II CATG 8 cut(s) 110, 398, 479, 538, 797, 850, 995, 1231
Hin6I GCGC 1 cut(s) 1036
HinP1I GCGC 1 cut(s) 1036
HinfI GANTC 5 cut(s) 515, 524, 817, 887, 1114
HpaII CCGG 3 cut(s) 330, 624, 1063
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 7 cut(s) 78, 192, 465, 972, 982, 1113, 1192
Hpy188III TCNNGA 5 cut(s) 233, 350, 415, 1082, 1147
Hpy8I GTNNAC 1 cut(s) 241
Hpy99I CGWCG 1 cut(s) 1046
HpyAV CCTTC 4 cut(s) 239, 419, 560, 950
HpyCH4III ACNGT 3 cut(s) 356, 605, 620
HpyCH4IV ACGT 3 cut(s) 925, 1012, 1280
HpyCH4V TGCA 8 cut(s) 110, 443, 593, 647, 932, 1204, 1233, 1265
HpyF10VI GCNNNNNNNGC 3 cut(s) 581, 590, 1035
HpyF3I CTNAG 7 cut(s) 24, 777, 801, 904, 1017, 1110, 1287
HpySE526I ACGT 3 cut(s) 925, 1012, 1280
Hsp92II CATG 8 cut(s) 110, 398, 479, 538, 797, 850, 995, 1231
HspAI GCGC 1 cut(s) 1036
Kzo9I GATC 5 cut(s) 448, 908, 934, 1024, 1054
LmnI GCTCC 3 cut(s) 531, 973, 1137
Lsp1109I GCAGC 4 cut(s) 271, 596, 1127, 1277
LweI GCATC 6 cut(s) 97, 408, 919, 1047, 1140, 1277
MabI ACCWGGT 1 cut(s) 202
MaeI CTAG 6 cut(s) 53, 198, 350, 452, 785, 999
MaeII ACGT 3 cut(s) 925, 1012, 1280
MaeIII GTNAC 6 cut(s) 277, 701, 796, 1088, 1182, 1243
MalI GATC 5 cut(s) 450, 910, 936, 1026, 1056
MboI GATC 5 cut(s) 448, 908, 934, 1024, 1054
MboII GAAGA 3 cut(s) 136, 829, 1108
MflI RGATCY 2 cut(s) 908, 934
MlyI GAGTC 1 cut(s) 1123
MnlI CCTC 9 cut(s) 535, 557, 651, 688, 772, 981, 997, 1012, 1119
Mph1103I ATGCAT 1 cut(s) 112
MroXI GAANNNNTTC 1 cut(s) 249
MseI TTAA 1 cut(s) 630
MspA1I CMGCKG 2 cut(s) 284, 404
MspI CCGG 3 cut(s) 330, 624, 1063
MspR9I CCNGG 4 cut(s) 204, 331, 624, 636
Mva1269I GAATGC 2 cut(s) 179, 1206
MvaI CCWGG 2 cut(s) 204, 636
MwoI GCNNNNNNNGC 3 cut(s) 581, 590, 1035
NciI CCSGG 2 cut(s) 331, 624
NcoI CCATGG 1 cut(s) 394
NdeI CATATG 1 cut(s) 67
NdeII GATC 5 cut(s) 448, 908, 934, 1024, 1054
NlaIII CATG 8 cut(s) 110, 398, 479, 538, 797, 850, 995, 1231
NlaIV GGNNCC 4 cut(s) 37, 271, 400, 910
NmuCI GTSAC 4 cut(s) 277, 701, 1088, 1182
NsiI ATGCAT 1 cut(s) 112
NspI RCATGY 2 cut(s) 538, 1231
PciI ACATGT 1 cut(s) 1227
PctI GAATGC 2 cut(s) 179, 1206
PdmI GAANNNNTTC 1 cut(s) 249
PfeI GAWTC 4 cut(s) 515, 524, 817, 887
PkrI GCNGC 5 cut(s) 286, 403, 586, 1142, 1267
PleI GAGTC 1 cut(s) 1122
PpsI GAGTC 1 cut(s) 1122
Ppu21I YACGTR 1 cut(s) 1013
PscI ACATGT 1 cut(s) 1227
PshAI GACNNNNGTC 1 cut(s) 357
Psp6I CCWGG 2 cut(s) 202, 634
PspEI GGTNACC 1 cut(s) 1243
PspFI CCCAGC 1 cut(s) 1238
PspGI CCWGG 2 cut(s) 202, 634
PspN4I GGNNCC 4 cut(s) 37, 271, 400, 910
PspPI GGNCC 4 cut(s) 230, 359, 544, 638
PsrI GAACNNNNNNTAC 2 cut(s) 754, 786
PstI CTGCAG 1 cut(s) 649
PsuI RGATCY 2 cut(s) 908, 934
SaqAI TTAA 1 cut(s) 630
SatI GCNGC 5 cut(s) 285, 402, 585, 1141, 1266
Sau3AI GATC 5 cut(s) 448, 908, 934, 1024, 1054
Sau96I GGNCC 4 cut(s) 230, 359, 544, 638
SbfI CCTGCAGG 1 cut(s) 649
SchI GAGTC 1 cut(s) 1123
ScrFI CCNGG 4 cut(s) 204, 331, 624, 636
SdaI CCTGCAGG 1 cut(s) 649
SexAI ACCWGGT 1 cut(s) 202
SfaNI GCATC 6 cut(s) 97, 408, 919, 1047, 1140, 1277
SfcI CTRYAG 2 cut(s) 82, 645
SinI GGWCC 3 cut(s) 230, 359, 638
Sse8387I CCTGCAGG 1 cut(s) 649
SsiI CCGC 5 cut(s) 95, 132, 282, 402, 718
SspI AATATT 3 cut(s) 120, 1102, 1175
SspMI CTAG 6 cut(s) 53, 198, 350, 452, 785, 999
StyD4I CCNGG 4 cut(s) 202, 329, 622, 634
StyI CCWWGG 2 cut(s) 255, 394
TaaI ACNGT 3 cut(s) 356, 605, 620
TaiI ACGT 3 cut(s) 928, 1015, 1283
TaqI TCGA 4 cut(s) 540, 959, 1041, 1105
TauI GCSGC 1 cut(s) 404
TfiI GAWTC 4 cut(s) 515, 524, 817, 887
Tru1I TTAA 1 cut(s) 630
Tru9I TTAA 1 cut(s) 630
TscAI CASTG 2 cut(s) 343, 361
TseFI GTSAC 4 cut(s) 277, 701, 1088, 1182
TseI GCWGC 4 cut(s) 284, 584, 1140, 1265
Tsp45I GTSAC 4 cut(s) 277, 701, 1088, 1182
TspDTI ATGAA 6 cut(s) 54, 159, 492, 759, 830, 1008
TspGWI ACGGA 1 cut(s) 611
TspRI CASTG 2 cut(s) 343, 361
VpaK11BI GGWCC 3 cut(s) 230, 359, 638
XagI CCTNNNNNAGG 1 cut(s) 645
XbaI TCTAGA 1 cut(s) 349
XceI RCATGY 2 cut(s) 538, 1231
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 249
XspI CTAG 6 cut(s) 53, 198, 350, 452, 785, 999
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.