Rw2G038170

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001085
Physical Location & Seq
Reverse (-)
63079477 .. 63083467
3991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G038170.1

Sequence Viewer

Length: 1545 bp
ATGAGTATTCAAGGTTTAGTAGTACTTCTTTTTCTGTCTGTTTTGAGTGTGAGTACTATTGGGAGCACTCTTGTTGAAAATGGTTCTACGAGTACCACTCATCATAAGTCCAAGGGAAAAAGATGGGCACTTCTAATTGCTGGTTCAAATGGTTACGATAACTATCGACACCAGGCTGATGTATGCCATGCATACCAGATACTTAAGAAAGGTGGACTCAAAGATGAAAACATTATTGTCTTCATGTATGATGATATTGCTAACAACACAGAAAACCCTAGACCTGGTGTCATTATCAACAAGCCAAACGGCAAGGATGTCTACCACGGAGTTCCCAAGGATTACACAGGAGATGATGTTAACTCACACAATTTCTACGCTGCTATTCTCGGAGAGAAAACTGCACTCAAAGGAGGAAGTGGCAAGGTTCTGGAGACTGGTCCAAATGACCATATTTTCATATATTACACTGATCATGGTGCTACAGGACTTCTTGGGATGCCTAGTGAAAGTGACTTTGTTTATGCTAAAGATCTGATACATGTGCTGAAAAAGAAGCACGCTGCTAAAGGTTACAAAAGCATGGTGTTTTACCTTGAAGCTTGTGAGTCTGGGAGTATGTTTGAGGGTTTGCTTCCAAAGAATATGAACATTTATGCTACTACAGCATCAAATGCGGAGGAGAGTAGTTATGCAACATACTGTGATGGTGACCCTGGTGTTCCTACAGAATTCGGTACTTGTTTGGGAGACTTGTATAGTATTTCCTGGTTGGAGGATTGCGATGCAAATGATCTGCGGAAAGAGACTTTTGGGGTTCAATATAAAAGGGTCCGAAGCAGAACAAATAAATCTCATGTTATGCAGTATGGAGATATGAGTCACATAAAGGAATTCCTCTACACTTACATGGGGGCAACTCCTAAAACTCATAGCTATGCTCCCTTCACAGCCTCGCCTTCGGAATCAATCTCAAGGACTGTTAGCCAGCGGGATGCAGCCCTCCTATATTTTCTGCAAAAGTTAAACAGAGCTCCAACTGGCTCTCATGAGAAGATCGAAGCTCAAAAGCAGCTACGTAAAGAAATTGCTCGAAGGAAACATGTGGACTACAGCATAACCAAAATAGGGGAGCTTCTGTTTGGGACCGAGAGGAGCTCAAAAGTATTGATGAATGTCAGGCCTTCGGGACAACCTCTAGTGGATGACTGGGAGTGCTTCAAGATGTTTATAAGGACTTATGAGAGTCGCTGTGGAAAGCTATCGAAATATGGAATGAAATACACGCGAGCTTTTGCCAACATGTGCAATGCCGGGGTCAGCCTAGAGAAACTTGTTGCAGTGTCTGATCAAGCTTGTTCTAAGAAACATAGTAATTACTATTACACAATATATTACTTTCATTACGCAAGAGAGAAATTTATTACTCAAACGAGGACGGGTAAAGCTATGGACATTAACATTTCTCATACATGTTCTATTTTATATAATATTTACCACTCTGAGGACTCATGTAACCATTTTGAGGACACATGTGGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

514

Amino Acids

57.73

Weight (kDa)

8.39

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 41 - 305 7.3e-117 Peptidase C13 family
Legum_prodom PF20985 357 - 453 2.6e-39 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1232
AccI GTMKAC 1 cut(s) 321
AccII CGCG 1 cut(s) 1288
AciI CCGC 3 cut(s) 677, 799, 991
AcsI RAATTY 3 cut(s) 731, 893, 1418
AfaI GTAC 4 cut(s) 24, 55, 94, 739
AfiI CCNNNNNNNGG 4 cut(s) 284, 1128, 1504, 1525
AflII CTTAAG 1 cut(s) 203
AflIII ACRYGT 5 cut(s) 541, 1102, 1302, 1472, 1532
AgsI TTSAA 6 cut(s) 11, 77, 147, 599, 821, 1222
AhdI GACNNNNNGTC 1 cut(s) 287
AjnI CCWGG 4 cut(s) 171, 283, 715, 767
Alw21I GWGCWC 3 cut(s) 68, 1036, 1160
Alw26I GTCTC 3 cut(s) 428, 744, 800
AlwNI CAGNNNCTG 1 cut(s) 1346
AoxI GGCC 1 cut(s) 1181
ApeKI GCWGC 4 cut(s) 380, 563, 998, 1072
ApoI RAATTY 3 cut(s) 731, 893, 1418
AspS9I GGNCC 3 cut(s) 440, 832, 1146
AsuC2I CCSGG 1 cut(s) 1315
AsuHPI GGTGA 1 cut(s) 722
AvaII GGWCC 3 cut(s) 440, 832, 1146
BaeGI GKGCMC 1 cut(s) 130
BanII GRGCYC 2 cut(s) 1036, 1160
BbsI GAAGAC 1 cut(s) 232
Bbv12I GWGCWC 3 cut(s) 68, 1036, 1160
BbvI GCAGC 4 cut(s) 367, 550, 1010, 1084
BccI CCATC 2 cut(s) 117, 701
BceAI ACGGC 1 cut(s) 325
BciT130I CCWGG 4 cut(s) 173, 285, 717, 769
BclI TGATCA 2 cut(s) 472, 1348
BcnI CCSGG 1 cut(s) 1315
BcoDI GTCTC 3 cut(s) 428, 744, 800
BfaI CTAG 4 cut(s) 279, 504, 1199, 1325
BfmI CTRYAG 4 cut(s) 483, 663, 726, 1111
BfrI CTTAAG 1 cut(s) 203
BglII AGATCT 1 cut(s) 532
BisI GCNGC 4 cut(s) 381, 564, 999, 1073
BlsI GCNGC 4 cut(s) 382, 565, 1000, 1074
BmcAI AGTACT 2 cut(s) 24, 55
Bme1390I CCNGG 5 cut(s) 173, 285, 717, 769, 1315
Bme18I GGWCC 3 cut(s) 440, 832, 1146
BmeRI GACNNNNNGTC 1 cut(s) 287
BmgT120I GGNCC 3 cut(s) 440, 832, 1146
BmiI GGNNCC 2 cut(s) 833, 1147
BmrFI CCNGG 5 cut(s) 173, 285, 717, 769, 1315
BmrI ACTGGG 1 cut(s) 1219
BmsI GCATC 4 cut(s) 489, 677, 775, 985
BmuI ACTGGG 1 cut(s) 1219
BpiI GAAGAC 1 cut(s) 232
BplI GAGNNNNNCTC 4 cut(s) 82, 114, 1142, 1174
BpmI CTGGAG 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 958
BpuMI CCSGG 1 cut(s) 1315
BsaAI YACGTR 1 cut(s) 1079
BsaBI GATNNNNATC 1 cut(s) 162
BsaJI CCNNGG 5 cut(s) 111, 325, 336, 715, 1314
BsaXI ACNNNNNCTCC 4 cut(s) 342, 372, 1124, 1154
Bsc4I CCNNNNNNNGG 4 cut(s) 284, 1128, 1504, 1525
Bse1I ACTGG 3 cut(s) 442, 1045, 1214
Bse3DI GCAATG 1 cut(s) 1315
Bse8I GATNNNNATC 1 cut(s) 162
BseBI CCWGG 4 cut(s) 173, 285, 717, 769
BseDI CCNNGG 5 cut(s) 111, 325, 336, 715, 1314
BseGI GGATG 4 cut(s) 322, 504, 1000, 1210
BseJI GATNNNNATC 1 cut(s) 162
BseLI CCNNNNNNNGG 4 cut(s) 284, 1128, 1504, 1525
BseMI GCAATG 1 cut(s) 1315
BseMII CTCAG 1 cut(s) 1494
BseNI ACTGG 3 cut(s) 442, 1045, 1214
BseRI GAGGAG 2 cut(s) 695, 1168
BseSI GKGCMC 1 cut(s) 130
BseXI GCAGC 4 cut(s) 367, 550, 1010, 1084
BsgI GTGCAG 1 cut(s) 387
Bsh1236I CGCG 1 cut(s) 1288
BshFI GGCC 1 cut(s) 1183
BsiHKAI GWGCWC 3 cut(s) 68, 1036, 1160
BsiSI CCGG 1 cut(s) 1314
BslFI GGGAC 2 cut(s) 1159, 1203
BslI CCNNNNNNNGG 4 cut(s) 284, 1128, 1504, 1525
BsmAI GTCTC 3 cut(s) 428, 744, 800
BsmFI GGGAC 2 cut(s) 1159, 1203
BsnI GGCC 1 cut(s) 1183
Bsp1286I GDGCHC 4 cut(s) 68, 130, 1036, 1160
Bsp143I GATC 5 cut(s) 472, 532, 793, 1056, 1348
BspACI CCGC 3 cut(s) 677, 799, 991
BspANI GGCC 1 cut(s) 1183
BspCNI CTCAG 1 cut(s) 1495
BspFNI CGCG 1 cut(s) 1288
BspHI TCATGA 1 cut(s) 1048
BspLI GGNNCC 2 cut(s) 833, 1147
BspTI CTTAAG 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 1315
BsrI ACTGG 3 cut(s) 442, 1045, 1214
BssECI CCNNGG 5 cut(s) 111, 325, 336, 715, 1314
BssMI GATC 5 cut(s) 472, 532, 793, 1056, 1348
BssT1I CCWWGG 2 cut(s) 111, 336
Bst2UI CCWGG 4 cut(s) 173, 285, 717, 769
Bst4CI ACNGT 2 cut(s) 704, 982
BstAFI CTTAAG 1 cut(s) 203
BstAPI GCANNNNNTGC 1 cut(s) 674
BstBAI YACGTR 1 cut(s) 1079
BstC8I GCNNGC 3 cut(s) 561, 989, 1290
BstDEI CTNAG 2 cut(s) 1362, 1503
BstDSI CCRYGG 1 cut(s) 325
BstEII GGTNACC 1 cut(s) 710
BstF5I GGATG 4 cut(s) 322, 504, 1000, 1210
BstFNI CGCG 1 cut(s) 1288
BstKTI GATC 5 cut(s) 475, 535, 796, 1059, 1351
BstMAI GTCTC 3 cut(s) 428, 744, 800
BstMBI GATC 5 cut(s) 472, 532, 793, 1056, 1348
BstMWI GCNNNNNNNGC 2 cut(s) 665, 674
BstNI CCWGG 4 cut(s) 173, 285, 717, 769
BstNSI RCATGY 5 cut(s) 545, 1106, 1306, 1476, 1536
BstPI GGTNACC 1 cut(s) 710
BstSCI CCNGG 5 cut(s) 171, 283, 715, 767, 1313
BstSFI CTRYAG 4 cut(s) 483, 663, 726, 1111
BstSLI GKGCMC 1 cut(s) 130
BstSNI TACGTA 1 cut(s) 1079
BstUI CGCG 1 cut(s) 1288
BstV1I GCAGC 4 cut(s) 367, 550, 1010, 1084
BstV2I GAAGAC 1 cut(s) 232
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
BsuRI GGCC 1 cut(s) 1183
BtgI CCRYGG 1 cut(s) 325
BtgZI GCGATG 1 cut(s) 798
BtsCI GGATG 4 cut(s) 322, 504, 1000, 1210
BtsI GCAGTG 1 cut(s) 1347
BtsIMutI CAGTG 2 cut(s) 468, 1347
Cac8I GCNNGC 3 cut(s) 561, 989, 1290
CaiI CAGNNNCTG 1 cut(s) 1346
CciI TCATGA 1 cut(s) 1048
Cfr13I GGNCC 3 cut(s) 440, 832, 1146
CsiI ACCWGGT 1 cut(s) 283
Csp6I GTAC 4 cut(s) 23, 54, 93, 738
CviQI GTAC 4 cut(s) 23, 54, 93, 738
DdeI CTNAG 2 cut(s) 1362, 1503
DpnI GATC 5 cut(s) 474, 534, 795, 1058, 1350
DpnII GATC 5 cut(s) 472, 532, 793, 1056, 1348
DriI GACNNNNNGTC 1 cut(s) 287
Eam1105I GACNNNNNGTC 1 cut(s) 287
Ecl136II GAGCTC 2 cut(s) 1034, 1158
Eco105I TACGTA 1 cut(s) 1079
Eco130I CCWWGG 2 cut(s) 111, 336
Eco147I AGGCCT 1 cut(s) 1183
Eco24I GRGCYC 2 cut(s) 1036, 1160
Eco47I GGWCC 3 cut(s) 440, 832, 1146
Eco53kI GAGCTC 2 cut(s) 1034, 1158
Eco91I GGTNACC 1 cut(s) 710
EcoICRI GAGCTC 2 cut(s) 1034, 1158
EcoO65I GGTNACC 1 cut(s) 710
EcoRI GAATTC 2 cut(s) 731, 893
EcoRII CCWGG 4 cut(s) 171, 283, 715, 767
EcoT14I CCWWGG 2 cut(s) 111, 336
EcoT22I ATGCAT 1 cut(s) 193
EcoT38I GRGCYC 2 cut(s) 1036, 1160
ErhI CCWWGG 2 cut(s) 111, 336
FaqI GGGAC 2 cut(s) 1159, 1203
FauI CCCGC 1 cut(s) 984
FbaI TGATCA 2 cut(s) 472, 1348
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 4 cut(s) 381, 564, 999, 1073
FokI GGATG 4 cut(s) 329, 511, 1007, 1217
FriOI GRGCYC 2 cut(s) 1036, 1160
Fsp4HI GCNGC 4 cut(s) 381, 564, 999, 1073
FspBI CTAG 4 cut(s) 279, 504, 1199, 1325
GluI GCNGC 4 cut(s) 381, 564, 999, 1073
GsuI CTGGAG 1 cut(s) 452
HaeIII GGCC 1 cut(s) 1183
HapII CCGG 1 cut(s) 1314
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 2 cut(s) 600, 1353
HinfI GANTC 6 cut(s) 216, 608, 880, 965, 1246, 1508
HpaI GTTAAC 1 cut(s) 361
HpaII CCGG 1 cut(s) 1314
HphI GGTGA 1 cut(s) 722
Hpy166II GTNNAC 4 cut(s) 215, 322, 361, 1108
Hpy188I TCNGA 6 cut(s) 392, 537, 836, 964, 1348, 1504
Hpy188III TCNNGA 4 cut(s) 431, 1049, 1188, 1222
Hpy8I GTNNAC 4 cut(s) 215, 322, 361, 1108
HpyAV CCTTC 4 cut(s) 955, 969, 1089, 1194
HpyCH4III ACNGT 2 cut(s) 704, 982
HpyCH4IV ACGT 1 cut(s) 1078
HpyCH4V TGCA 9 cut(s) 191, 404, 695, 788, 865, 998, 1018, 1308, 1340
HpyF10VI GCNNNNNNNGC 2 cut(s) 665, 674
HpyF3I CTNAG 2 cut(s) 1362, 1503
HpySE526I ACGT 1 cut(s) 1078
Ksp22I TGATCA 2 cut(s) 472, 1348
KspAI GTTAAC 1 cut(s) 361
Kzo9I GATC 5 cut(s) 472, 532, 793, 1056, 1348
LmnI GCTCC 5 cut(s) 63, 946, 1039, 1132, 1155
Lsp1109I GCAGC 4 cut(s) 367, 550, 1010, 1084
LweI GCATC 4 cut(s) 489, 677, 775, 985
MabI ACCWGGT 1 cut(s) 283
MaeI CTAG 4 cut(s) 279, 504, 1199, 1325
MaeII ACGT 1 cut(s) 1078
MaeIII GTNAC 6 cut(s) 152, 512, 572, 710, 881, 1514
MalI GATC 5 cut(s) 474, 534, 795, 1058, 1350
MboI GATC 5 cut(s) 472, 532, 793, 1056, 1348
MboII GAAGA 2 cut(s) 232, 1066
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 4 cut(s) 68, 130, 1036, 1160
MluCI AATT 8 cut(s) 135, 370, 731, 893, 1086, 1375, 1418, 1540
MlyI GAGTC 5 cut(s) 210, 617, 889, 1255, 1502
MmeI TCCRAC 2 cut(s) 753, 1061
Mph1103I ATGCAT 1 cut(s) 193
MseI TTAA 4 cut(s) 204, 360, 1025, 1458
MslI CAYNNNNRTG 2 cut(s) 908, 936
MspA1I CMGCKG 1 cut(s) 991
MspCI CTTAAG 1 cut(s) 203
MspI CCGG 1 cut(s) 1314
MspR9I CCNGG 5 cut(s) 173, 285, 717, 769, 1315
MvaI CCWGG 4 cut(s) 173, 285, 717, 769
MvnI CGCG 1 cut(s) 1288
MwoI GCNNNNNNNGC 2 cut(s) 665, 674
NciI CCSGG 1 cut(s) 1315
NdeII GATC 5 cut(s) 472, 532, 793, 1056, 1348
NlaIV GGNNCC 2 cut(s) 833, 1147
NmuCI GTSAC 3 cut(s) 512, 710, 881
NsiI ATGCAT 1 cut(s) 193
NspI RCATGY 5 cut(s) 545, 1106, 1306, 1476, 1536
PagI TCATGA 1 cut(s) 1048
PceI AGGCCT 1 cut(s) 1183
PciI ACATGT 5 cut(s) 541, 1102, 1302, 1472, 1532
PfeI GAWTC 1 cut(s) 965
PkrI GCNGC 4 cut(s) 382, 565, 1000, 1074
PleI GAGTC 5 cut(s) 210, 616, 888, 1254, 1502
PpsI GAGTC 5 cut(s) 210, 616, 888, 1254, 1502
Ppu21I YACGTR 1 cut(s) 1079
PscI ACATGT 5 cut(s) 541, 1102, 1302, 1472, 1532
PsiI TTATAA 1 cut(s) 1232
Psp124BI GAGCTC 2 cut(s) 1036, 1160
Psp6I CCWGG 4 cut(s) 171, 283, 715, 767
PspEI GGTNACC 1 cut(s) 710
PspGI CCWGG 4 cut(s) 171, 283, 715, 767
PspN4I GGNNCC 2 cut(s) 833, 1147
PspPI GGNCC 3 cut(s) 440, 832, 1146
PstNI CAGNNNCTG 1 cut(s) 1346
PsuI RGATCY 1 cut(s) 532
RsaI GTAC 4 cut(s) 24, 55, 94, 739
RsaNI GTAC 4 cut(s) 23, 54, 93, 738
RseI CAYNNNNRTG 2 cut(s) 908, 936
SacI GAGCTC 2 cut(s) 1036, 1160
SaqAI TTAA 4 cut(s) 204, 360, 1025, 1458
SatI GCNGC 4 cut(s) 381, 564, 999, 1073
Sau3AI GATC 5 cut(s) 472, 532, 793, 1056, 1348
Sau96I GGNCC 3 cut(s) 440, 832, 1146
ScaI AGTACT 2 cut(s) 24, 55
SchI GAGTC 5 cut(s) 210, 617, 889, 1255, 1502
ScrFI CCNGG 5 cut(s) 173, 285, 717, 769, 1315
SduI GDGCHC 4 cut(s) 68, 130, 1036, 1160
SexAI ACCWGGT 1 cut(s) 283
SfaNI GCATC 4 cut(s) 489, 677, 775, 985
SfcI CTRYAG 4 cut(s) 483, 663, 726, 1111
SinI GGWCC 3 cut(s) 440, 832, 1146
SmiMI CAYNNNNRTG 2 cut(s) 908, 936
SmlI CTYRAG 2 cut(s) 203, 973
SmoI CTYRAG 2 cut(s) 203, 973
SnaBI TACGTA 1 cut(s) 1079
Sse9I AATT 8 cut(s) 135, 370, 731, 893, 1086, 1375, 1418, 1540
SseBI AGGCCT 1 cut(s) 1183
SsiI CCGC 3 cut(s) 677, 799, 991
SspI AATATT 1 cut(s) 1492
SspMI CTAG 4 cut(s) 279, 504, 1199, 1325
SstI GAGCTC 2 cut(s) 1036, 1160
StuI AGGCCT 1 cut(s) 1183
StyD4I CCNGG 5 cut(s) 171, 283, 715, 767, 1313
StyI CCWWGG 2 cut(s) 111, 336
TaaI ACNGT 2 cut(s) 704, 982
TaiI ACGT 1 cut(s) 1081
TaqI TCGA 4 cut(s) 166, 1059, 1093, 1265
TaqII GACCGA 1 cut(s) 1163
TasI AATT 8 cut(s) 135, 370, 731, 893, 1086, 1375, 1418, 1540
TatI WGTACW 2 cut(s) 22, 53
TfiI GAWTC 1 cut(s) 965
Tru1I TTAA 4 cut(s) 204, 360, 1025, 1458
Tru9I TTAA 4 cut(s) 204, 360, 1025, 1458
TscAI CASTG 2 cut(s) 475, 1347
TseFI GTSAC 3 cut(s) 512, 710, 881
TseI GCWGC 4 cut(s) 380, 563, 998, 1072
Tsp45I GTSAC 3 cut(s) 512, 710, 881
TspDTI ATGAA 7 cut(s) 232, 240, 448, 662, 1187, 1292, 1391
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 2 cut(s) 475, 1347
Vha464I CTTAAG 1 cut(s) 203
VpaK11BI GGWCC 3 cut(s) 440, 832, 1146
XapI RAATTY 3 cut(s) 731, 893, 1418
XceI RCATGY 5 cut(s) 545, 1106, 1306, 1476, 1536
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 4 cut(s) 279, 504, 1199, 1325
ZrmI AGTACT 2 cut(s) 24, 55
Zsp2I ATGCAT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.