MD14G1092500.v1.1

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001085
Physical Location & Seq
Reverse (-)
13828312 .. 13832775
4464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1092500.v1.1.491

Sequence Viewer

Length: 1422 bp
ATGAGAACATTTTTAATATTGATTGCGATTTTGTTAAGTTTGAGTTGTGAAAGCGTCGCAAATGGATTCAAGTTGATGCCATCAGAATTTCCGGAGGATTTCATCATTCATGTTAATACTGCCTCATCATCTTCTTCTACTGTGGCTGATCAGCAAAATGGAACCAGATGGGCAGTCTTAGTTGCTGGGTCGTTTGGTTATGGCAACTATAGGCATCAGGCCGATGTGTGTCATGCATATCAAATATTGAAGAAAGGTGGACTAAAAGATGAAAACATCATTGTTTTTATGTACGATGACATCGCAAACAACGTTGAGAACCCTAGACCTGGTGTCATCATCAATAAGCCAGATGGCCCTGATGTTTACAAAGGGGTTCCCAAGGATTACACTGGAGAAAATGTTACTGCTGCCAATCTTTATGCTGTTATTCTTGGAAATAAAACAGCTCTCAGCGGGGGCAGTGGCAAGATTCTGGACAGCGGTCCAAATGACAATGTTTTTATATATTATACCGACCATGGCGCCTCTGGGATAATTGGAATGCCTGAAGGCGATTACGTGTATGCTAACGATCTCGTGGACGTTCTGAAACAGAAGCATGAAGCTAAGGGTTACAAAAAAATGGTATTTTACCTAGAATCTTGTGAATCTGGGAGCATGTTCGAGGGTCTGCTTCCAAGTAATATAAGTATCTATGCGACTACAGCGGCAAATGCAACTCAAGATAGTTGGGCGACATATTGTCCTGGTTTTGATCCTGCTCCTCCTGCAGGATATGACACTTGTTTGGGAGACTTGTATAGTATTTCTTGGATGGAAGATTGCGATGTCAAAGATTTACGAAGAGAAACTTTTGCAAACCAATATGAAACGGTTAGAAATAGAACCGCAAATACTAGTGAAGGACTTGGGTCTCATGTTACTCAATACGGAAATTTGAACCAAGCCAAGGATTTACTCTTCTCTTACTTGGGTTCAGATGACAACTCTACTACTTACACCAACACTAAATCCTCACCATCATTCTTAGGGCCAGTTTTTAACCAACGTGATGCAGACCTCCTCCACTTTTTGCACAAGGTACGTAAAGCTCCTGATGGCTCTCATGAGAAGCATGAAGCTAAACAGAAACTATCTGCTGAAATGTATCGTCGGGAGCGCATCGACGACAATATAAATCAAATCGCGCAACTAGTGTTTCGATATGATTCTAGCTCAAAAATGAAAAATATTCGGCGTAACGGGCAAGCTCTTGTGGATGACTGGAACTGCTTTAAGACTTTTGTGAAAACTTACGAGAAATATTGTGGAGCACTCTCAAATTATGGAATGCAATACACAAGAATTATTGCCAACATGTGCAATTATGGGGTTACCATGGAGCAAATGACTGCAGCATCAACGAAAACTTGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

474

Amino Acids

52.52

Weight (kDa)

5.63

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 56 - 326 7e-113 Peptidase C13 family
Legum_prodom PF20985 377 - 472 7e-33 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 524
AccII CGCG 1 cut(s) 1190
AccIII TCCGGA 1 cut(s) 91
AciI CCGC 4 cut(s) 456, 483, 710, 891
AclI AACGTT 1 cut(s) 312
AclWI GGATC 1 cut(s) 752
AcsI RAATTY 2 cut(s) 86, 937
AcuI CTGAAG 1 cut(s) 570
AcyI GRCGYC 1 cut(s) 525
AfaI GTAC 2 cut(s) 293, 1086
AfiI CCNNNNNNNGG 3 cut(s) 329, 773, 952
AflIII ACRYGT 2 cut(s) 561, 1359
AgsI TTSAA 3 cut(s) 70, 250, 943
AhdI GACNNNNNGTC 2 cut(s) 332, 744
AhlI ACTAGT 2 cut(s) 899, 1195
AjnI CCWGG 2 cut(s) 328, 748
AluBI AGCT 6 cut(s) 449, 608, 1094, 1124, 1218, 1253
AluI AGCT 6 cut(s) 449, 608, 1094, 1124, 1218, 1253
Alw21I GWGCWC 1 cut(s) 1318
Alw26I GTCTC 2 cut(s) 789, 921
AlwI GGATC 1 cut(s) 752
Aor13HI TCCGGA 1 cut(s) 91
AoxI GGCC 3 cut(s) 219, 355, 1034
ApeKI GCWGC 2 cut(s) 410, 1397
ApoI RAATTY 2 cut(s) 86, 937
AspLEI GCGC 3 cut(s) 527, 1164, 1192
AspS9I GGNCC 3 cut(s) 356, 485, 1034
AsuHPI GGTGA 1 cut(s) 1011
AvaII GGWCC 1 cut(s) 485
BanI GGYRCC 1 cut(s) 524
BauI CACGAG 1 cut(s) 578
Bbv12I GWGCWC 1 cut(s) 1318
BbvI GCAGC 2 cut(s) 397, 1409
BccI CCATC 6 cut(s) 88, 162, 347, 811, 1030, 1094
BciT130I CCWGG 2 cut(s) 330, 750
BclI TGATCA 1 cut(s) 148
BcoDI GTCTC 2 cut(s) 789, 921
BcuI ACTAGT 2 cut(s) 899, 1195
BfaI CTAG 5 cut(s) 324, 638, 900, 1196, 1215
BfmI CTRYAG 4 cut(s) 208, 705, 771, 1395
BfoI RGCGCY 1 cut(s) 528
BisI GCNGC 3 cut(s) 411, 711, 1398
BlsI GCNGC 3 cut(s) 412, 712, 1399
Bme1390I CCNGG 2 cut(s) 330, 750
Bme18I GGWCC 1 cut(s) 485
BmeRI GACNNNNNGTC 2 cut(s) 332, 744
BmgT120I GGNCC 3 cut(s) 356, 485, 1034
BmiI GGNNCC 3 cut(s) 163, 378, 526
BmrFI CCNGG 2 cut(s) 330, 750
BmsI GCATC 5 cut(s) 66, 223, 1045, 1173, 1409
BoxI GACNNNNGTC 2 cut(s) 483, 913
BpmI CTGGAG 1 cut(s) 414
Bpu10I CCTNAGC 1 cut(s) 609
BpuEI CTTGAG 1 cut(s) 708
BsaAI YACGTR 2 cut(s) 562, 1088
BsaHI GRCGYC 1 cut(s) 525
BsaI GGTCTC 1 cut(s) 921
BsaJI CCNNGG 4 cut(s) 381, 520, 951, 1380
BsaWI WCCGGW 1 cut(s) 91
BsaXI ACNNNNNCTCC 2 cut(s) 387, 417
Bsc4I CCNNNNNNNGG 3 cut(s) 329, 773, 952
Bse1I ACTGG 3 cut(s) 397, 1037, 1271
BseAI TCCGGA 1 cut(s) 91
BseBI CCWGG 2 cut(s) 330, 750
BseDI CCNNGG 4 cut(s) 381, 520, 951, 1380
BseGI GGATG 2 cut(s) 822, 1267
BseLI CCNNNNNNNGG 3 cut(s) 329, 773, 952
BseMII CTCAG 1 cut(s) 466
BseNI ACTGG 3 cut(s) 397, 1037, 1271
BseRI GAGGAG 2 cut(s) 756, 1055
BseXI GCAGC 2 cut(s) 397, 1409
BseYI CCCAGC 1 cut(s) 185
Bsh1236I CGCG 1 cut(s) 1190
BshFI GGCC 3 cut(s) 221, 357, 1036
BshNI GGYRCC 1 cut(s) 524
BsiHKAI GWGCWC 1 cut(s) 1318
BsiSI CCGG 1 cut(s) 92
BslI CCNNNNNNNGG 3 cut(s) 329, 773, 952
BsmAI GTCTC 2 cut(s) 789, 921
BsmI GAATGC 2 cut(s) 549, 1338
BsnI GGCC 3 cut(s) 221, 357, 1036
Bso31I GGTCTC 1 cut(s) 921
Bsp1286I GDGCHC 1 cut(s) 1318
Bsp13I TCCGGA 1 cut(s) 91
Bsp143I GATC 3 cut(s) 148, 574, 757
Bsp19I CCATGG 2 cut(s) 520, 1380
BspACI CCGC 4 cut(s) 456, 483, 710, 891
BspANI GGCC 3 cut(s) 221, 357, 1036
BspCNI CTCAG 1 cut(s) 465
BspEI TCCGGA 1 cut(s) 91
BspFNI CGCG 1 cut(s) 1190
BspHI TCATGA 1 cut(s) 1108
BspLI GGNNCC 3 cut(s) 163, 378, 526
BspMAI CTGCAG 2 cut(s) 775, 1399
BspPI GGATC 1 cut(s) 752
BspT107I GGYRCC 1 cut(s) 524
BspTNI GGTCTC 1 cut(s) 921
BsrI ACTGG 3 cut(s) 397, 1037, 1271
BssECI CCNNGG 4 cut(s) 381, 520, 951, 1380
BssMI GATC 3 cut(s) 148, 574, 757
BssNI GRCGYC 1 cut(s) 525
BssSI CACGAG 1 cut(s) 578
BssT1I CCWWGG 4 cut(s) 381, 520, 951, 1380
Bst2BI CACGAG 1 cut(s) 578
Bst2UI CCWGG 2 cut(s) 330, 750
Bst4CI ACNGT 2 cut(s) 142, 877
Bst6I CTCTTC 2 cut(s) 841, 968
BstACI GRCGYC 1 cut(s) 525
BstBAI YACGTR 2 cut(s) 562, 1088
BstC8I GCNNGC 1 cut(s) 1251
BstDEI CTNAG 5 cut(s) 178, 452, 609, 1030, 1419
BstDSI CCRYGG 2 cut(s) 520, 1380
BstEII GGTNACC 1 cut(s) 1375
BstENI CCTNNNNNAGG 1 cut(s) 771
BstF5I GGATG 2 cut(s) 822, 1267
BstFNI CGCG 1 cut(s) 1190
BstH2I RGCGCY 1 cut(s) 528
BstHHI GCGC 3 cut(s) 527, 1164, 1192
BstKTI GATC 3 cut(s) 151, 577, 760
BstMAI GTCTC 2 cut(s) 789, 921
BstMBI GATC 3 cut(s) 148, 574, 757
BstMWI GCNNNNNNNGC 4 cut(s) 707, 716, 770, 1246
BstNI CCWGG 2 cut(s) 330, 750
BstNSI RCATGY 2 cut(s) 664, 1363
BstPAI GACNNNNGTC 2 cut(s) 483, 913
BstPI GGTNACC 1 cut(s) 1375
BstSCI CCNGG 2 cut(s) 328, 748
BstSFI CTRYAG 4 cut(s) 208, 705, 771, 1395
BstSNI TACGTA 1 cut(s) 1088
BstUI CGCG 1 cut(s) 1190
BstV1I GCAGC 2 cut(s) 397, 1409
BsuRI GGCC 3 cut(s) 221, 357, 1036
BtgI CCRYGG 2 cut(s) 520, 1380
BtgZI GCGATG 2 cut(s) 286, 843
BtsCI GGATG 2 cut(s) 822, 1267
BtsI GCAGTG 1 cut(s) 469
BtsIMutI CAGTG 2 cut(s) 390, 469
Cac8I GCNNGC 1 cut(s) 1251
CciI TCATGA 1 cut(s) 1108
CfoI GCGC 3 cut(s) 527, 1164, 1192
Cfr13I GGNCC 3 cut(s) 356, 485, 1034
CseI GACGC 1 cut(s) 43
CsiI ACCWGGT 1 cut(s) 328
Csp6I GTAC 2 cut(s) 292, 1085
CviQI GTAC 2 cut(s) 292, 1085
DdeI CTNAG 5 cut(s) 178, 452, 609, 1030, 1419
DinI GGCGCC 1 cut(s) 526
DpnI GATC 3 cut(s) 150, 576, 759
DpnII GATC 3 cut(s) 148, 574, 757
DriI GACNNNNNGTC 2 cut(s) 332, 744
Eam1104I CTCTTC 2 cut(s) 841, 968
Eam1105I GACNNNNNGTC 2 cut(s) 332, 744
EarI CTCTTC 2 cut(s) 841, 968
Eco105I TACGTA 1 cut(s) 1088
Eco130I CCWWGG 4 cut(s) 381, 520, 951, 1380
Eco31I GGTCTC 1 cut(s) 921
Eco47I GGWCC 1 cut(s) 485
Eco57I CTGAAG 1 cut(s) 570
Eco91I GGTNACC 1 cut(s) 1375
EcoNI CCTNNNNNAGG 1 cut(s) 771
EcoO65I GGTNACC 1 cut(s) 1375
EcoRII CCWGG 2 cut(s) 328, 748
EcoT14I CCWWGG 4 cut(s) 381, 520, 951, 1380
EcoT22I ATGCAT 1 cut(s) 238
EgeI GGCGCC 1 cut(s) 526
EheI GGCGCC 1 cut(s) 526
ErhI CCWWGG 4 cut(s) 381, 520, 951, 1380
FalI AAGNNNNNCTT 2 cut(s) 838, 870
FauI CCCGC 1 cut(s) 449
FbaI TGATCA 1 cut(s) 148
Fnu4HI GCNGC 3 cut(s) 411, 711, 1398
FokI GGATG 2 cut(s) 829, 1274
Fsp4HI GCNGC 3 cut(s) 411, 711, 1398
FspBI CTAG 5 cut(s) 324, 638, 900, 1196, 1215
GlaI GCGC 3 cut(s) 526, 1163, 1191
GluI GCNGC 3 cut(s) 411, 711, 1398
GsaI CCCAGC 1 cut(s) 189
GsuI CTGGAG 1 cut(s) 414
HaeII RGCGCY 1 cut(s) 528
HaeIII GGCC 3 cut(s) 221, 357, 1036
HapII CCGG 1 cut(s) 92
HgaI GACGC 1 cut(s) 43
HhaI GCGC 3 cut(s) 527, 1164, 1192
Hin1I GRCGYC 1 cut(s) 525
Hin6I GCGC 3 cut(s) 525, 1162, 1190
HinP1I GCGC 3 cut(s) 525, 1162, 1190
HinfI GANTC 5 cut(s) 66, 472, 641, 650, 1211
HpaII CCGG 1 cut(s) 92
HphI GGTGA 1 cut(s) 1011
Hpy166II GTNNAC 3 cut(s) 260, 367, 583
Hpy188I TCNGA 3 cut(s) 85, 591, 982
Hpy188III TCNNGA 6 cut(s) 92, 476, 725, 1097, 1109, 1157
Hpy8I GTNNAC 3 cut(s) 260, 367, 583
Hpy99I CGWCG 3 cut(s) 59, 1158, 1172
HpyAV CCTTC 2 cut(s) 545, 899
HpyCH4III ACNGT 2 cut(s) 142, 877
HpyCH4IV ACGT 5 cut(s) 312, 561, 585, 1051, 1087
HpyCH4V TGCA 9 cut(s) 236, 719, 773, 860, 1058, 1078, 1336, 1365, 1397
HpyF10VI GCNNNNNNNGC 4 cut(s) 707, 716, 770, 1246
HpyF3I CTNAG 5 cut(s) 178, 452, 609, 1030, 1419
HpySE526I ACGT 5 cut(s) 312, 561, 585, 1051, 1087
Hsp92I GRCGYC 1 cut(s) 525
HspAI GCGC 3 cut(s) 525, 1162, 1190
KasI GGCGCC 1 cut(s) 524
Kpn2I TCCGGA 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 3 cut(s) 148, 574, 757
LmnI GCTCC 6 cut(s) 657, 769, 1099, 1159, 1313, 1384
Lsp1109I GCAGC 2 cut(s) 397, 1409
LweI GCATC 5 cut(s) 66, 223, 1045, 1173, 1409
MabI ACCWGGT 1 cut(s) 328
MaeI CTAG 5 cut(s) 324, 638, 900, 1196, 1215
MaeII ACGT 5 cut(s) 312, 561, 585, 1051, 1087
MaeIII GTNAC 5 cut(s) 403, 614, 922, 1241, 1375
MalI GATC 3 cut(s) 150, 576, 759
MboI GATC 3 cut(s) 148, 574, 757
MboII GAAGA 6 cut(s) 123, 126, 262, 833, 858, 955
MhlI GDGCHC 1 cut(s) 1318
MluCI AATT 6 cut(s) 86, 537, 937, 1324, 1347, 1366
Mly113I GGCGCC 1 cut(s) 525
MnlI CCTC 8 cut(s) 88, 133, 538, 661, 777, 1027, 1073, 1076
Mph1103I ATGCAT 1 cut(s) 238
MroI TCCGGA 1 cut(s) 91
MseI TTAA 5 cut(s) 14, 35, 114, 1044, 1278
MspA1I CMGCKG 3 cut(s) 456, 483, 710
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 2 cut(s) 330, 750
Mva1269I GAATGC 2 cut(s) 549, 1338
MvaI CCWGG 2 cut(s) 330, 750
MvnI CGCG 1 cut(s) 1190
MwoI GCNNNNNNNGC 4 cut(s) 707, 716, 770, 1246
NarI GGCGCC 1 cut(s) 525
NcoI CCATGG 2 cut(s) 520, 1380
NdeII GATC 3 cut(s) 148, 574, 757
NlaIV GGNNCC 3 cut(s) 163, 378, 526
NsiI ATGCAT 1 cut(s) 238
NspI RCATGY 2 cut(s) 664, 1363
PagI TCATGA 1 cut(s) 1108
PciI ACATGT 1 cut(s) 1359
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 2 cut(s) 549, 1338
PfeI GAWTC 5 cut(s) 66, 472, 641, 650, 1211
PkrI GCNGC 3 cut(s) 412, 712, 1399
PluTI GGCGCC 1 cut(s) 528
Ppu21I YACGTR 2 cut(s) 562, 1088
PscI ACATGT 1 cut(s) 1359
PshAI GACNNNNGTC 2 cut(s) 483, 913
Psp1406I AACGTT 1 cut(s) 312
Psp6I CCWGG 2 cut(s) 328, 748
PspEI GGTNACC 1 cut(s) 1375
PspFI CCCAGC 1 cut(s) 185
PspGI CCWGG 2 cut(s) 328, 748
PspN4I GGNNCC 3 cut(s) 163, 378, 526
PspPI GGNCC 3 cut(s) 356, 485, 1034
PsrI GAACNNNNNNTAC 2 cut(s) 880, 912
PstI CTGCAG 2 cut(s) 775, 1399
RsaI GTAC 2 cut(s) 293, 1086
RsaNI GTAC 2 cut(s) 292, 1085
SaqAI TTAA 5 cut(s) 14, 35, 114, 1044, 1278
SatI GCNGC 3 cut(s) 411, 711, 1398
Sau3AI GATC 3 cut(s) 148, 574, 757
Sau96I GGNCC 3 cut(s) 356, 485, 1034
SbfI CCTGCAGG 1 cut(s) 775
ScrFI CCNGG 2 cut(s) 330, 750
SdaI CCTGCAGG 1 cut(s) 775
SduI GDGCHC 1 cut(s) 1318
SexAI ACCWGGT 1 cut(s) 328
SfaNI GCATC 5 cut(s) 66, 223, 1045, 1173, 1409
SfcI CTRYAG 4 cut(s) 208, 705, 771, 1395
SfoI GGCGCC 1 cut(s) 526
SinI GGWCC 1 cut(s) 485
SmlI CTYRAG 1 cut(s) 723
SmoI CTYRAG 1 cut(s) 723
SnaBI TACGTA 1 cut(s) 1088
SpeI ACTAGT 2 cut(s) 899, 1195
Sse8387I CCTGCAGG 1 cut(s) 775
Sse9I AATT 6 cut(s) 86, 537, 937, 1324, 1347, 1366
SsiI CCGC 4 cut(s) 456, 483, 710, 891
SspDI GGCGCC 1 cut(s) 524
SspI AATATT 4 cut(s) 18, 246, 1234, 1307
SspMI CTAG 5 cut(s) 324, 638, 900, 1196, 1215
StyD4I CCNGG 2 cut(s) 328, 748
StyI CCWWGG 4 cut(s) 381, 520, 951, 1380
TaaI ACNGT 2 cut(s) 142, 877
TaiI ACGT 5 cut(s) 315, 564, 588, 1054, 1090
TaqI TCGA 3 cut(s) 666, 1167, 1204
TasI AATT 6 cut(s) 86, 537, 937, 1324, 1347, 1366
TauI GCSGC 1 cut(s) 713
TfiI GAWTC 5 cut(s) 66, 472, 641, 650, 1211
Tru1I TTAA 5 cut(s) 14, 35, 114, 1044, 1278
Tru9I TTAA 5 cut(s) 14, 35, 114, 1044, 1278
TscAI CASTG 2 cut(s) 397, 469
TseI GCWGC 2 cut(s) 410, 1397
TspDTI ATGAA 7 cut(s) 91, 98, 285, 618, 885, 1134, 1241
TspGWI ACGGA 1 cut(s) 948
TspRI CASTG 2 cut(s) 397, 469
VpaK11BI GGWCC 1 cut(s) 485
XagI CCTNNNNNAGG 1 cut(s) 771
XapI RAATTY 2 cut(s) 86, 937
XceI RCATGY 2 cut(s) 664, 1363
XcmI CCANNNNNNNNNTGG 1 cut(s) 527
XspI CTAG 5 cut(s) 324, 638, 900, 1196, 1215
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.