MD04G1025000.v1.1

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001085
Physical Location & Seq
Reverse (-)
2918675 .. 2921048
2374 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1025000.v1.1.491

Sequence Viewer

Length: 1389 bp
ATGGCTAATCATGGTTATTGTGGGGTTTTACTATCTCTTACTTTGTTAAGTTTGGCTATTCATGGAAGCTTCTGCTTCCCTGAAATTAATGGAAATAACAAGGGCTCTTCACGTACCACTACTGATAAGGGAAAAACATGGGCAGTTCTGGTTGCAGGATCAAGTGGTTACGACAACTACAGGCACCAGGCTGACATATGCCATGCGTACCAGATACTTAAGAAAGGAGGACTGAAAGACGAGAACATCATCGTTTTCATGTACGATGATATCGCGTACAACTCGGAAAATCCTCGAAAAGGTGTCATCATCAACAAGCCAAATGGTCATGATGTTTATAAAGGAGTTCCCAAGGATTATACAGGAGATCATGTTAACGCACGCAATCTCTATGCTGTTATTCTCGGAGACAAAAGTGCTCTCACCGGAGGAAGTGGCAAGGTTCTAAGTAGCGGTCCGAATGACCATGTTTTCATATATTATGCAGACCATGGTTCTGTAGGATTACTTGGGATGCCTAGCGATTATGTTTATGCGGAAGATCTCATACGTGTACTGAAGAAGAAGAATGCTTCTAAAGGTTACAAAAGCATGGTTTTTTACATTGAAGCTTGCGAGGCAGGGAGCATGTTTGAGGGCCTCCTTTCAAGTAATCTGAATATTTATGCTACCACCGCGTCGAATGCAGAAGAGAGTAGTTATGGAACATATTGTCCCGGTGACCAATCAGTTCCTGCCGAGTTTGATACTTGTTTGGGAGACTTGTATAGCATTTCCTGGATGGAGGACTGTGACATAAGTGATTTGCATAAAGAAACTTTGGAGAATCAATATGAAAGGGTTCGAAGAAGAACAACTAATTCGCATGTTATGCAGTACGGAGATATGAGTCATAAACAGGAGTTCCTCTTTGCTTACATGGGTGCTGATCTTTCTAATCGTAGCCATACTTCCACCAGCGATATCTCTTCACCCTCGATCTCAAGGGCTGTTGACCAACGTGACACGAAGCTCCTCTACTTTCAACAAAAGCTGCAAAGAGCTCCTACTGGATCTCAAGAGAAACAGGGTGCTCAGAAGCAGCTGCTGCTTGAAATTGCTCATAGGAAAAACGTGGACTATAGCATAACGAAACTAGGGGAGGCTTTGTTCGGACACGAGAAGAGCTCAAACGTTTTAATGAATGTTAGGCCACAGGGCCAACCTGTGGTAGATAATTGGGACTGCTTCAAGAACTTTTTGAATATTTATGAGAAGTACTGTGGACATTTATCTGCATATGGAATGAAGTACACACGAGCTATAGCCAACATATGCAATGCTGGGATTACCACAGAGAAAATGGTTGCAGCATCTGATCAAACTTGTGCCAACAAACCCAATGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

463

Amino Acids

51.19

Weight (kDa)

6.57

Isoelectric Point (pI)

38.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 46 - 308 5.7e-118 Peptidase C13 family
Legum_prodom PF20985 360 - 456 1.4e-36 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 183
AccB7I CCANNNNNTGG 1 cut(s) 1207
AccII CGCG 2 cut(s) 275, 677
AciI CCGC 3 cut(s) 453, 536, 675
AclI AACGTT 1 cut(s) 1173
AclWI GGATC 2 cut(s) 166, 1060
AcuI CTGAAG 1 cut(s) 578
AfaI GTAC 8 cut(s) 115, 209, 263, 278, 555, 878, 1259, 1292
AfiI CCNNNNNNNGG 2 cut(s) 299, 1207
AflII CTTAAG 1 cut(s) 218
AflIII ACRYGT 1 cut(s) 550
AgsI TTSAA 6 cut(s) 608, 648, 1025, 1094, 1231, 1243
AjnI CCWGG 2 cut(s) 186, 776
AloI GAACNNNNNNTCC 4 cut(s) 697, 729, 1133, 1165
AluBI AGCT 8 cut(s) 69, 611, 1012, 1033, 1043, 1084, 1167, 1301
AluI AGCT 8 cut(s) 69, 611, 1012, 1033, 1043, 1084, 1167, 1301
Alw21I GWGCWC 4 cut(s) 421, 1045, 1075, 1169
Alw26I GTCTC 2 cut(s) 402, 753
AlwI GGATC 2 cut(s) 166, 1060
AlwNI CAGNNNCTG 3 cut(s) 734, 1087, 1355
AoxI GGCC 3 cut(s) 637, 1190, 1198
ApeKI GCWGC 5 cut(s) 1033, 1081, 1084, 1087, 1349
ArsI GACNNNNNNTTYG 1 cut(s) 1368
AseI ATTAAT 1 cut(s) 87
Asp700I GAANNNNTTC 1 cut(s) 840
AspS9I GGNCC 3 cut(s) 455, 637, 1198
AsuC2I CCSGG 1 cut(s) 717
AsuHPI GGTGA 3 cut(s) 415, 731, 963
AsuII TTCGAA 1 cut(s) 844
AvaII GGWCC 1 cut(s) 455
BanI GGYRCC 1 cut(s) 183
BanII GRGCYC 3 cut(s) 107, 1045, 1169
BarI GAAGNNNNNNTAC 6 cut(s) 531, 563, 934, 966, 1001, 1033
BauI CACGAG 2 cut(s) 1157, 1296
Bbv12I GWGCWC 4 cut(s) 421, 1045, 1075, 1169
BbvI GCAGC 5 cut(s) 1020, 1071, 1074, 1093, 1361
BccI CCATC 1 cut(s) 775
BciT130I CCWGG 2 cut(s) 188, 778
BclI TGATCA 1 cut(s) 1357
BcnI CCSGG 1 cut(s) 717
BcoDI GTCTC 2 cut(s) 402, 753
BfaI CTAG 3 cut(s) 519, 1136, 1387
BfmI CTRYAG 4 cut(s) 178, 498, 1120, 1302
BfrI CTTAAG 1 cut(s) 218
BglII AGATCT 1 cut(s) 541
BisI GCNGC 5 cut(s) 1034, 1082, 1085, 1088, 1350
BlsI GCNGC 5 cut(s) 1035, 1083, 1086, 1089, 1351
BmcAI AGTACT 1 cut(s) 1259
Bme1390I CCNGG 3 cut(s) 188, 717, 778
Bme18I GGWCC 1 cut(s) 455
BmgT120I GGNCC 3 cut(s) 455, 637, 1198
BmiI GGNNCC 1 cut(s) 185
BmrFI CCNGG 3 cut(s) 188, 717, 778
BmsI GCATC 2 cut(s) 504, 1361
BplI GAGNNNNNCTC 2 cut(s) 1151, 1183
Bpu14I TTCGAA 1 cut(s) 844
BpuEI CTTGAG 2 cut(s) 967, 1041
BpuMI CCSGG 1 cut(s) 717
BsaAI YACGTR 2 cut(s) 113, 551
BsaJI CCNNGG 2 cut(s) 351, 490
BsaWI WCCGGW 1 cut(s) 425
BsaXI ACNNNNNCTCC 4 cut(s) 357, 387, 1133, 1163
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 1207
Bse1I ACTGG 1 cut(s) 1054
Bse3DI GCAATG 1 cut(s) 1324
BseBI CCWGG 2 cut(s) 188, 778
BseDI CCNNGG 2 cut(s) 351, 490
BseGI GGATG 2 cut(s) 519, 786
BseLI CCNNNNNNNGG 2 cut(s) 299, 1207
BseMI GCAATG 1 cut(s) 1324
BseMII CTCAG 1 cut(s) 1088
BseNI ACTGG 1 cut(s) 1054
BseRI GAGGAG 1 cut(s) 1004
BseXI GCAGC 5 cut(s) 1020, 1071, 1074, 1093, 1361
BseYI CCCAGC 1 cut(s) 1322
Bsh1236I CGCG 2 cut(s) 275, 677
BshFI GGCC 3 cut(s) 639, 1192, 1200
BshNI GGYRCC 1 cut(s) 183
BsiHKAI GWGCWC 4 cut(s) 421, 1045, 1075, 1169
BsiSI CCGG 2 cut(s) 426, 717
BslFI GGGAC 2 cut(s) 699, 1235
BslI CCNNNNNNNGG 2 cut(s) 299, 1207
BsmAI GTCTC 2 cut(s) 402, 753
BsmFI GGGAC 2 cut(s) 699, 1235
BsmI GAATGC 2 cut(s) 574, 688
BsnI GGCC 3 cut(s) 639, 1192, 1200
Bsp119I TTCGAA 1 cut(s) 844
Bsp1286I GDGCHC 5 cut(s) 107, 421, 1045, 1075, 1169
Bsp143I GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
Bsp19I CCATGG 1 cut(s) 490
BspACI CCGC 3 cut(s) 453, 536, 675
BspANI GGCC 3 cut(s) 639, 1192, 1200
BspCNI CTCAG 1 cut(s) 1087
BspFNI CGCG 2 cut(s) 275, 677
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 1 cut(s) 185
BspPI GGATC 2 cut(s) 166, 1060
BspQI GCTCTTC 2 cut(s) 112, 1157
BspT104I TTCGAA 1 cut(s) 844
BspT107I GGYRCC 1 cut(s) 183
BspTI CTTAAG 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 1324
BsrI ACTGG 1 cut(s) 1054
BssECI CCNNGG 2 cut(s) 351, 490
BssMI GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
BssSI CACGAG 2 cut(s) 1157, 1296
BssT1I CCWWGG 2 cut(s) 351, 490
Bst2BI CACGAG 2 cut(s) 1157, 1296
Bst2UI CCWGG 2 cut(s) 188, 778
Bst4CI ACNGT 2 cut(s) 791, 1262
Bst6I CTCTTC 4 cut(s) 112, 684, 973, 1157
BstAFI CTTAAG 1 cut(s) 218
BstAPI GCANNNNNTGC 2 cut(s) 871, 1087
BstBAI YACGTR 2 cut(s) 113, 551
BstBI TTCGAA 1 cut(s) 844
BstC8I GCNNGC 2 cut(s) 382, 613
BstDEI CTNAG 2 cut(s) 446, 1074
BstDSI CCRYGG 1 cut(s) 490
BstEII GGTNACC 1 cut(s) 719
BstENI CCTNNNNNAGG 1 cut(s) 297
BstF5I GGATG 2 cut(s) 519, 786
BstFNI CGCG 2 cut(s) 275, 677
BstKTI GATC 7 cut(s) 161, 370, 544, 931, 981, 1055, 1360
BstMAI GTCTC 2 cut(s) 402, 753
BstMBI GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
BstMWI GCNNNNNNNGC 5 cut(s) 617, 674, 683, 871, 1087
BstNI CCWGG 2 cut(s) 188, 778
BstNSI RCATGY 2 cut(s) 631, 869
BstPI GGTNACC 1 cut(s) 719
BstSCI CCNGG 3 cut(s) 186, 715, 776
BstSFI CTRYAG 4 cut(s) 178, 498, 1120, 1302
BstUI CGCG 2 cut(s) 275, 677
BstV1I GCAGC 5 cut(s) 1020, 1071, 1074, 1093, 1361
BstX2I RGATCY 2 cut(s) 541, 1052
BstYI RGATCY 2 cut(s) 541, 1052
BsuRI GGCC 3 cut(s) 639, 1192, 1200
BtgI CCRYGG 1 cut(s) 490
BtsCI GGATG 2 cut(s) 519, 786
Cac8I GCNNGC 2 cut(s) 382, 613
CaiI CAGNNNCTG 3 cut(s) 734, 1087, 1355
CciI TCATGA 1 cut(s) 328
Cfr13I GGNCC 3 cut(s) 455, 637, 1198
CpoI CGGWCCG 1 cut(s) 455
CseI GACGC 1 cut(s) 666
Csp6I GTAC 8 cut(s) 114, 208, 262, 277, 554, 877, 1258, 1291
CspI CGGWCCG 1 cut(s) 455
CviQI GTAC 8 cut(s) 114, 208, 262, 277, 554, 877, 1258, 1291
DdeI CTNAG 2 cut(s) 446, 1074
DpnI GATC 7 cut(s) 160, 369, 543, 930, 980, 1054, 1359
DpnII GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
Eam1104I CTCTTC 4 cut(s) 112, 684, 973, 1157
EarI CTCTTC 4 cut(s) 112, 684, 973, 1157
Ecl136II GAGCTC 2 cut(s) 1043, 1167
Eco130I CCWWGG 2 cut(s) 351, 490
Eco24I GRGCYC 3 cut(s) 107, 1045, 1169
Eco32I GATATC 2 cut(s) 271, 964
Eco47I GGWCC 1 cut(s) 455
Eco53kI GAGCTC 2 cut(s) 1043, 1167
Eco57I CTGAAG 1 cut(s) 578
Eco91I GGTNACC 1 cut(s) 719
EcoICRI GAGCTC 2 cut(s) 1043, 1167
EcoNI CCTNNNNNAGG 1 cut(s) 297
EcoO109I RGGNCCY 1 cut(s) 637
EcoO65I GGTNACC 1 cut(s) 719
EcoRII CCWGG 2 cut(s) 186, 776
EcoRV GATATC 2 cut(s) 271, 964
EcoT14I CCWWGG 2 cut(s) 351, 490
EcoT38I GRGCYC 3 cut(s) 107, 1045, 1169
ErhI CCWWGG 2 cut(s) 351, 490
FaqI GGGAC 2 cut(s) 699, 1235
FauNDI CATATG 3 cut(s) 197, 1279, 1313
FbaI TGATCA 1 cut(s) 1357
Fnu4HI GCNGC 5 cut(s) 1034, 1082, 1085, 1088, 1350
FokI GGATG 2 cut(s) 526, 793
FriOI GRGCYC 3 cut(s) 107, 1045, 1169
Fsp4HI GCNGC 5 cut(s) 1034, 1082, 1085, 1088, 1350
FspBI CTAG 3 cut(s) 519, 1136, 1387
GluI GCNGC 5 cut(s) 1034, 1082, 1085, 1088, 1350
GsaI CCCAGC 1 cut(s) 1326
HaeIII GGCC 3 cut(s) 639, 1192, 1200
HapII CCGG 2 cut(s) 426, 717
HgaI GACGC 1 cut(s) 666
HincII GTYRAC 2 cut(s) 376, 994
HindII GTYRAC 2 cut(s) 376, 994
HindIII AAGCTT 2 cut(s) 67, 609
HinfI GANTC 2 cut(s) 826, 889
HpaI GTTAAC 1 cut(s) 376
HpaII CCGG 2 cut(s) 426, 717
HphI GGTGA 3 cut(s) 415, 731, 963
Hpy166II GTNNAC 6 cut(s) 376, 554, 994, 1117, 1265, 1293
Hpy188I TCNGA 7 cut(s) 286, 407, 459, 657, 1077, 1154, 1357
Hpy188III TCNNGA 3 cut(s) 329, 1058, 1231
Hpy8I GTNNAC 6 cut(s) 376, 554, 994, 1117, 1265, 1293
Hpy99I CGWCG 1 cut(s) 682
HpyCH4III ACNGT 2 cut(s) 791, 1262
HpyCH4IV ACGT 5 cut(s) 112, 550, 1000, 1113, 1173
HpyCH4V TGCA 9 cut(s) 155, 485, 686, 808, 874, 1036, 1277, 1317, 1349
HpyF10VI GCNNNNNNNGC 5 cut(s) 617, 674, 683, 871, 1087
HpyF3I CTNAG 2 cut(s) 446, 1074
HpySE526I ACGT 5 cut(s) 112, 550, 1000, 1113, 1173
Ksp22I TGATCA 1 cut(s) 1357
KspAI GTTAAC 1 cut(s) 376
Kzo9I GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
LguI GCTCTTC 2 cut(s) 112, 1157
LmnI GCTCC 3 cut(s) 624, 1017, 1048
Lsp1109I GCAGC 5 cut(s) 1020, 1071, 1074, 1093, 1361
LweI GCATC 2 cut(s) 504, 1361
MaeI CTAG 3 cut(s) 519, 1136, 1387
MaeII ACGT 5 cut(s) 112, 550, 1000, 1113, 1173
MaeIII GTNAC 5 cut(s) 167, 581, 719, 791, 1001
MalI GATC 7 cut(s) 160, 369, 543, 930, 980, 1054, 1359
MboI GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
MflI RGATCY 2 cut(s) 541, 1052
MhlI GDGCHC 5 cut(s) 107, 421, 1045, 1075, 1169
MluCI AATT 4 cut(s) 84, 859, 1095, 1216
MlyI GAGTC 1 cut(s) 898
MroXI GAANNNNTTC 1 cut(s) 840
MseI TTAA 5 cut(s) 47, 87, 219, 375, 1178
MspA1I CMGCKG 1 cut(s) 1084
MspCI CTTAAG 1 cut(s) 218
MspI CCGG 2 cut(s) 426, 717
MspR9I CCNGG 3 cut(s) 188, 717, 778
Mva1269I GAATGC 2 cut(s) 574, 688
MvaI CCWGG 2 cut(s) 188, 778
MvnI CGCG 2 cut(s) 275, 677
MwoI GCNNNNNNNGC 5 cut(s) 617, 674, 683, 871, 1087
NciI CCSGG 1 cut(s) 717
NcoI CCATGG 1 cut(s) 490
NdeI CATATG 3 cut(s) 197, 1279, 1313
NdeII GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
NlaIV GGNNCC 1 cut(s) 185
NmeAIII GCCGAG 1 cut(s) 763
NmuCI GTSAC 3 cut(s) 719, 791, 1001
NspI RCATGY 2 cut(s) 631, 869
NspV TTCGAA 1 cut(s) 844
PagI TCATGA 1 cut(s) 328
PciSI GCTCTTC 2 cut(s) 112, 1157
PctI GAATGC 2 cut(s) 574, 688
PdmI GAANNNNTTC 1 cut(s) 840
PfeI GAWTC 1 cut(s) 826
PflMI CCANNNNNTGG 1 cut(s) 1207
PfoI TCCNGGA 1 cut(s) 776
PkrI GCNGC 5 cut(s) 1035, 1083, 1086, 1089, 1351
PleI GAGTC 1 cut(s) 897
PpsI GAGTC 1 cut(s) 897
Ppu21I YACGTR 2 cut(s) 113, 551
PshBI ATTAAT 1 cut(s) 87
PsiI TTATAA 1 cut(s) 339
Psp124BI GAGCTC 2 cut(s) 1045, 1169
Psp1406I AACGTT 1 cut(s) 1173
Psp6I CCWGG 2 cut(s) 186, 776
PspEI GGTNACC 1 cut(s) 719
PspFI CCCAGC 1 cut(s) 1322
PspGI CCWGG 2 cut(s) 186, 776
PspN4I GGNNCC 1 cut(s) 185
PspPI GGNCC 3 cut(s) 455, 637, 1198
PstNI CAGNNNCTG 3 cut(s) 734, 1087, 1355
PsuI RGATCY 2 cut(s) 541, 1052
PvuII CAGCTG 1 cut(s) 1084
RsaI GTAC 8 cut(s) 115, 209, 263, 278, 555, 878, 1259, 1292
RsaNI GTAC 8 cut(s) 114, 208, 262, 277, 554, 877, 1258, 1291
Rsr2I CGGWCCG 1 cut(s) 455
RsrII CGGWCCG 1 cut(s) 455
SacI GAGCTC 2 cut(s) 1045, 1169
SapI GCTCTTC 2 cut(s) 112, 1157
SaqAI TTAA 5 cut(s) 47, 87, 219, 375, 1178
SatI GCNGC 5 cut(s) 1034, 1082, 1085, 1088, 1350
Sau3AI GATC 7 cut(s) 158, 367, 541, 928, 978, 1052, 1357
Sau96I GGNCC 3 cut(s) 455, 637, 1198
ScaI AGTACT 1 cut(s) 1259
SchI GAGTC 1 cut(s) 898
ScrFI CCNGG 3 cut(s) 188, 717, 778
SduI GDGCHC 5 cut(s) 107, 421, 1045, 1075, 1169
SfaNI GCATC 2 cut(s) 504, 1361
SfcI CTRYAG 4 cut(s) 178, 498, 1120, 1302
SfuI TTCGAA 1 cut(s) 844
SinI GGWCC 1 cut(s) 455
SmlI CTYRAG 3 cut(s) 218, 982, 1056
SmoI CTYRAG 3 cut(s) 218, 982, 1056
Sse9I AATT 4 cut(s) 84, 859, 1095, 1216
SsiI CCGC 3 cut(s) 453, 536, 675
SspI AATATT 2 cut(s) 661, 1246
SspMI CTAG 3 cut(s) 519, 1136, 1387
SstI GAGCTC 2 cut(s) 1045, 1169
StyD4I CCNGG 3 cut(s) 186, 715, 776
StyI CCWWGG 2 cut(s) 351, 490
TaaI ACNGT 2 cut(s) 791, 1262
TaiI ACGT 5 cut(s) 115, 553, 1003, 1116, 1176
TaqI TCGA 4 cut(s) 295, 680, 844, 977
TasI AATT 4 cut(s) 84, 859, 1095, 1216
TatI WGTACW 3 cut(s) 553, 1257, 1290
TfiI GAWTC 1 cut(s) 826
Tru1I TTAA 5 cut(s) 47, 87, 219, 375, 1178
Tru9I TTAA 5 cut(s) 47, 87, 219, 375, 1178
TseFI GTSAC 3 cut(s) 719, 791, 1001
TseI GCWGC 5 cut(s) 1033, 1081, 1084, 1087, 1349
Tsp45I GTSAC 3 cut(s) 719, 791, 1001
TspDTI ATGAA 6 cut(s) 50, 247, 463, 849, 1196, 1301
TspGWI ACGGA 1 cut(s) 894
Van91I CCANNNNNTGG 1 cut(s) 1207
Vha464I CTTAAG 1 cut(s) 218
VpaK11BI GGWCC 1 cut(s) 455
VspI ATTAAT 1 cut(s) 87
XagI CCTNNNNNAGG 1 cut(s) 297
XceI RCATGY 2 cut(s) 631, 869
XcmI CCANNNNNNNNNTGG 1 cut(s) 1339
XmnI GAANNNNTTC 1 cut(s) 840
XspI CTAG 3 cut(s) 519, 1136, 1387
ZrmI AGTACT 1 cut(s) 1259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.