Prupe.3G024900_v2.0.a1

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001085
Physical Location & Seq
Forward (+)
1825750 .. 1828324
2575 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G024900.1

Sequence Viewer

Length: 1410 bp
ATGGCTATTCTAAGTTGTGGAGTATTGCTATCCCTCGCTTTGCTAAGTTTGAGTATTGGAATTAACTTGTGCCTCCCTGAAAATGGAAAAGAAATAGATTTCAGTGCTCATGGTGAAAATGCCTCTCCAAGTACCACTAAGAAGGATAAGGGAAAAAGATGGGCAGTTCTAATTGCCGGGTCAAATGGTTACTACAACTATAGGCATCAGGCTGATATATGCCATGCATACCAGATACTGAAAAAAGGTGGACTGAAAGATGAGAACATCATTGTTTTCATGTATGATGACATTGCGCATGACCCCGAAAACCCTAGACAAGGTGTCATCATCAACAAGCCAAAGGGCCATGATGTTTACAAAGGAGTTCCCAAGGATTATACAGGGGCAGATGTTAACTCAAAAAATTTCTATGCTGTTATTCTTGGAAACAAAAGGAACCTCACTGGAGGTAGTGGCAAGGTTTTGAAAAGTGGTCCAAATGACCATGTTTTCATCTACTATGCAGACCATGGTTCTGCAGGATTACTTGGGATGCCTAGTGGGGATGATGCTGTTTATGCTAAAGATCTTATCCATGTGCTAAAGAAGAAGCATGCTTCTAAGGGTTTCAAAAGCATGGTATTCTACGTCGAAGCTTGCGAGTCGGGGAGCATATTTGAGGGCCTCCTTCCAAATAAAATGAATATTTATGCTACTACCGCAGCAAATGCAGAAGAGAGTAGTTATGGAACATATTGTCCAGGTGAACCTGAAGTTCCTGAGGAGTATGATACCTGTTTGGGAGACTTGTATAGTATTTCCTGGTTGGAGGATTGTGACAGCAATGATTTGCGCAAAGAAACTTTGGAGAAGCAATATGAAAGGGTTCGAAGAAGAACAAATAAGTCTCATGTTATGCAGTATGGAGATATGAGCCATAGGAAAGAGTTCCTCTTCACTTACATGGGTGCAGATCTTGCGAACGATAGCTATACTTCCATCGGCGATATCTCTTCACCCTCAAGGGCTGTTAACCAACGTGACACAAACCTCCTTTATTTTCAGCACAAGTTCCTTAGAGCTCCTGCCGGATCTCATGCAAAACTGGAAGCTCGAAAGCAGCTGCTTCATGAAATTGCTCATAGGAAACATGTGGACTATAGCATCCATAAAATAGGGGAGCTTTTGTTTGGATACCAGAAGAGCTCAAACGTATTGATGAACGGTAGGCCTCAGGGGCAACCTGTAGTAGACGATTGGGACTGCTTCAAGAAGTTTCTGAGGATTTATAAGAACTACTGTGGACATCTATCAACATATGGAATGAAATACACACGAGCTATAGCCAACATATGCAATGCTGGGGTAACCCTGGAGAAAATGGTTGCAGCTTCTGATCAAGCTTGTTCCAAGAAACTCCATGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

470

Amino Acids

52.33

Weight (kDa)

8.43

Isoelectric Point (pI)

42.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1272
Acc16I TGCGCA 2 cut(s) 297, 836
AccI GTMKAC 1 cut(s) 1233
AciI CCGC 1 cut(s) 702
AclWI GGATC 1 cut(s) 1081
AcsI RAATTY 1 cut(s) 406
AcuI CTGAAG 1 cut(s) 774
AfaI GTAC 1 cut(s) 133
AfiI CCNNNNNNNGG 2 cut(s) 83, 320
AflIII ACRYGT 1 cut(s) 1132
AgsI TTSAA 3 cut(s) 469, 613, 1252
AhdI GACNNNNNGTC 1 cut(s) 323
AjnI CCWGG 3 cut(s) 742, 803, 1353
AloI GAACNNNNNNTCC 2 cut(s) 724, 756
Alw21I GWGCWC 3 cut(s) 109, 1066, 1190
Alw26I GTCTC 2 cut(s) 780, 894
AlwI GGATC 1 cut(s) 1081
AlwNI CAGNNNCTG 2 cut(s) 238, 1376
AoxI GGCC 3 cut(s) 346, 664, 1211
ApeKI GCWGC 4 cut(s) 704, 1102, 1105, 1370
ApoI RAATTY 1 cut(s) 406
Asp700I GAANNNNTTC 2 cut(s) 867, 929
AspLEI GCGC 2 cut(s) 298, 837
AspS9I GGNCC 3 cut(s) 346, 476, 664
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 3 cut(s) 125, 758, 990
AsuII TTCGAA 1 cut(s) 871
AvaII GGWCC 1 cut(s) 476
AxyI CCTNAGG 2 cut(s) 762, 1215
BanII GRGCYC 2 cut(s) 1066, 1190
BauI CACGAG 1 cut(s) 1317
Bbv12I GWGCWC 3 cut(s) 109, 1066, 1190
BbvI GCAGC 4 cut(s) 716, 1092, 1114, 1382
BccI CCATC 2 cut(s) 153, 989
BciT130I CCWGG 3 cut(s) 744, 805, 1355
BciVI GTATCC 1 cut(s) 1169
BclI TGATCA 1 cut(s) 1378
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 2 cut(s) 780, 894
BfaI CTAG 3 cut(s) 315, 540, 1408
BfmI CTRYAG 5 cut(s) 199, 519, 1141, 1227, 1323
BfuI GTATCC 1 cut(s) 1169
BglI GCCNNNNNGGC 1 cut(s) 1219
BglII AGATCT 2 cut(s) 568, 955
BisI GCNGC 4 cut(s) 705, 1103, 1106, 1371
BlsI GCNGC 4 cut(s) 706, 1104, 1107, 1372
Bme1390I CCNGG 4 cut(s) 178, 744, 805, 1355
Bme18I GGWCC 1 cut(s) 476
BmeRI GACNNNNNGTC 1 cut(s) 323
BmgT120I GGNCC 3 cut(s) 346, 476, 664
BmiI GGNNCC 1 cut(s) 440
BmrFI CCNGG 4 cut(s) 178, 744, 805, 1355
BmsI GCATC 4 cut(s) 214, 525, 541, 1155
BpmI CTGGAG 2 cut(s) 468, 1376
Bpu14I TTCGAA 1 cut(s) 871
BpuEI CTTGAG 1 cut(s) 988
BpuMI CCSGG 1 cut(s) 178
BsaJI CCNNGG 3 cut(s) 372, 511, 1353
BsaXI ACNNNNNCTCC 2 cut(s) 1154, 1184
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 320
Bse1I ACTGG 2 cut(s) 451, 1092
Bse21I CCTNAGG 2 cut(s) 762, 1215
Bse3DI GCAATG 3 cut(s) 291, 832, 1345
BseBI CCWGG 3 cut(s) 744, 805, 1355
BseDI CCNNGG 3 cut(s) 372, 511, 1353
BseGI GGATG 3 cut(s) 540, 553, 1146
BseLI CCNNNNNNNGG 2 cut(s) 83, 320
BseMI GCAATG 3 cut(s) 291, 832, 1345
BseMII CTCAG 3 cut(s) 753, 1229, 1253
BseNI ACTGG 2 cut(s) 451, 1092
BseRI GAGGAG 1 cut(s) 779
BseXI GCAGC 4 cut(s) 716, 1092, 1114, 1382
BseYI CCCAGC 1 cut(s) 1343
BsgI GTGCAG 1 cut(s) 972
BshFI GGCC 3 cut(s) 348, 666, 1213
BsiHKAI GWGCWC 3 cut(s) 109, 1066, 1190
BsiSI CCGG 2 cut(s) 177, 1071
BslFI GGGAC 1 cut(s) 1256
BslI CCNNNNNNNGG 2 cut(s) 83, 320
BsmAI GTCTC 2 cut(s) 780, 894
BsmFI GGGAC 1 cut(s) 1256
BsnI GGCC 3 cut(s) 348, 666, 1213
Bsp119I TTCGAA 1 cut(s) 871
Bsp1286I GDGCHC 3 cut(s) 109, 1066, 1190
Bsp143I GATC 4 cut(s) 568, 955, 1073, 1378
Bsp19I CCATGG 1 cut(s) 511
BspACI CCGC 1 cut(s) 702
BspANI GGCC 3 cut(s) 348, 666, 1213
BspCNI CTCAG 3 cut(s) 754, 1228, 1254
BspHI TCATGA 1 cut(s) 1111
BspLI GGNNCC 1 cut(s) 440
BspMAI CTGCAG 1 cut(s) 523
BspPI GGATC 1 cut(s) 1081
BspQI GCTCTTC 1 cut(s) 1178
BspT104I TTCGAA 1 cut(s) 871
BsrDI GCAATG 3 cut(s) 291, 832, 1345
BsrI ACTGG 2 cut(s) 451, 1092
BssECI CCNNGG 3 cut(s) 372, 511, 1353
BssMI GATC 4 cut(s) 568, 955, 1073, 1378
BssSI CACGAG 1 cut(s) 1317
BssT1I CCWWGG 2 cut(s) 372, 511
Bst2BI CACGAG 1 cut(s) 1317
Bst2UI CCWGG 3 cut(s) 744, 805, 1355
Bst4CI ACNGT 2 cut(s) 1208, 1283
Bst6I CTCTTC 4 cut(s) 711, 941, 1000, 1178
BstAPI GCANNNNNTGC 2 cut(s) 710, 959
BstBI TTCGAA 1 cut(s) 871
BstC8I GCNNGC 2 cut(s) 597, 640
BstDEI CTNAG 8 cut(s) 11, 44, 138, 603, 762, 1058, 1215, 1262
BstDSI CCRYGG 1 cut(s) 511
BstEII GGTNACC 1 cut(s) 1348
BstENI CCTNNNNNAGG 1 cut(s) 318
BstF5I GGATG 3 cut(s) 540, 553, 1146
BstHHI GCGC 2 cut(s) 298, 837
BstKTI GATC 4 cut(s) 571, 958, 1076, 1381
BstMAI GTCTC 2 cut(s) 780, 894
BstMBI GATC 4 cut(s) 568, 955, 1073, 1378
BstMWI GCNNNNNNNGC 5 cut(s) 560, 701, 710, 959, 1219
BstNI CCWGG 3 cut(s) 744, 805, 1355
BstNSI RCATGY 2 cut(s) 599, 1136
BstPI GGTNACC 1 cut(s) 1348
BstSCI CCNGG 4 cut(s) 176, 742, 803, 1353
BstSFI CTRYAG 5 cut(s) 199, 519, 1141, 1227, 1323
BstV1I GCAGC 4 cut(s) 716, 1092, 1114, 1382
BstX2I RGATCY 3 cut(s) 568, 955, 1073
BstYI RGATCY 3 cut(s) 568, 955, 1073
Bsu36I CCTNAGG 2 cut(s) 762, 1215
BsuI GTATCC 1 cut(s) 1169
BsuRI GGCC 3 cut(s) 348, 666, 1213
BtgI CCRYGG 1 cut(s) 511
BtsCI GGATG 3 cut(s) 540, 553, 1146
BtsIMutI CAGTG 2 cut(s) 109, 444
Cac8I GCNNGC 2 cut(s) 597, 640
CaiI CAGNNNCTG 2 cut(s) 238, 1376
CciI TCATGA 1 cut(s) 1111
CfoI GCGC 2 cut(s) 298, 837
Cfr13I GGNCC 3 cut(s) 346, 476, 664
Csp6I GTAC 1 cut(s) 132
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 8 cut(s) 11, 44, 138, 603, 762, 1058, 1215, 1262
DpnI GATC 4 cut(s) 570, 957, 1075, 1380
DpnII GATC 4 cut(s) 568, 955, 1073, 1378
DriI GACNNNNNGTC 1 cut(s) 323
Eam1104I CTCTTC 4 cut(s) 711, 941, 1000, 1178
Eam1105I GACNNNNNGTC 1 cut(s) 323
EarI CTCTTC 4 cut(s) 711, 941, 1000, 1178
Ecl136II GAGCTC 2 cut(s) 1064, 1188
Eco130I CCWWGG 2 cut(s) 372, 511
Eco147I AGGCCT 1 cut(s) 1213
Eco24I GRGCYC 2 cut(s) 1066, 1190
Eco32I GATATC 1 cut(s) 991
Eco47I GGWCC 1 cut(s) 476
Eco53kI GAGCTC 2 cut(s) 1064, 1188
Eco57I CTGAAG 1 cut(s) 774
Eco81I CCTNAGG 2 cut(s) 762, 1215
Eco91I GGTNACC 1 cut(s) 1348
EcoICRI GAGCTC 2 cut(s) 1064, 1188
EcoNI CCTNNNNNAGG 1 cut(s) 318
EcoO109I RGGNCCY 1 cut(s) 664
EcoO65I GGTNACC 1 cut(s) 1348
EcoRII CCWGG 3 cut(s) 742, 803, 1353
EcoRV GATATC 1 cut(s) 991
EcoT14I CCWWGG 2 cut(s) 372, 511
EcoT22I ATGCAT 1 cut(s) 229
EcoT38I GRGCYC 2 cut(s) 1066, 1190
ErhI CCWWGG 2 cut(s) 372, 511
FaqI GGGAC 1 cut(s) 1256
FauNDI CATATG 2 cut(s) 1300, 1334
FbaI TGATCA 1 cut(s) 1378
FblI GTMKAC 1 cut(s) 1233
Fnu4HI GCNGC 4 cut(s) 705, 1103, 1106, 1371
FokI GGATG 3 cut(s) 547, 560, 1133
FriOI GRGCYC 2 cut(s) 1066, 1190
Fsp4HI GCNGC 4 cut(s) 705, 1103, 1106, 1371
FspBI CTAG 3 cut(s) 315, 540, 1408
FspI TGCGCA 2 cut(s) 297, 836
GlaI GCGC 2 cut(s) 297, 836
GluI GCNGC 4 cut(s) 705, 1103, 1106, 1371
GsaI CCCAGC 1 cut(s) 1347
GsuI CTGGAG 2 cut(s) 468, 1376
HaeIII GGCC 3 cut(s) 348, 666, 1213
HapII CCGG 2 cut(s) 177, 1071
HhaI GCGC 2 cut(s) 298, 837
Hin6I GCGC 2 cut(s) 296, 835
HinP1I GCGC 2 cut(s) 296, 835
HincII GTYRAC 2 cut(s) 397, 1015
HindII GTYRAC 2 cut(s) 397, 1015
HindIII AAGCTT 2 cut(s) 636, 1383
HinfI GANTC 1 cut(s) 644
HpaI GTTAAC 2 cut(s) 397, 1015
HpaII CCGG 2 cut(s) 177, 1071
HphI GGTGA 3 cut(s) 125, 758, 990
Hpy166II GTNNAC 8 cut(s) 251, 358, 397, 749, 1015, 1138, 1234, 1286
Hpy188I TCNGA 2 cut(s) 1263, 1378
Hpy188III TCNNGA 3 cut(s) 761, 1112, 1252
Hpy8I GTNNAC 8 cut(s) 251, 358, 397, 749, 1015, 1138, 1234, 1286
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 2 cut(s) 136, 680
HpyCH4III ACNGT 2 cut(s) 1208, 1283
HpyCH4IV ACGT 3 cut(s) 630, 1021, 1194
HpyCH4V TGCA 9 cut(s) 227, 506, 521, 713, 901, 953, 1082, 1338, 1370
HpyF10VI GCNNNNNNNGC 5 cut(s) 560, 701, 710, 959, 1219
HpyF3I CTNAG 8 cut(s) 11, 44, 138, 603, 762, 1058, 1215, 1262
HpySE526I ACGT 3 cut(s) 630, 1021, 1194
HspAI GCGC 2 cut(s) 296, 835
Ksp22I TGATCA 1 cut(s) 1378
KspAI GTTAAC 2 cut(s) 397, 1015
Kzo9I GATC 4 cut(s) 568, 955, 1073, 1378
LguI GCTCTTC 1 cut(s) 1178
LmnI GCTCC 3 cut(s) 651, 1069, 1162
Lsp1109I GCAGC 4 cut(s) 716, 1092, 1114, 1382
LweI GCATC 4 cut(s) 214, 525, 541, 1155
MaeI CTAG 3 cut(s) 315, 540, 1408
MaeII ACGT 3 cut(s) 630, 1021, 1194
MaeIII GTNAC 4 cut(s) 188, 818, 1022, 1348
MalI GATC 4 cut(s) 570, 957, 1075, 1380
MboI GATC 4 cut(s) 568, 955, 1073, 1378
MboII GAAGA 7 cut(s) 601, 728, 885, 888, 928, 987, 1195
MflI RGATCY 3 cut(s) 568, 955, 1073
MhlI GDGCHC 3 cut(s) 109, 1066, 1190
MluCI AATT 4 cut(s) 60, 171, 406, 1116
MlyI GAGTC 1 cut(s) 653
MmeI TCCRAC 1 cut(s) 789
Mph1103I ATGCAT 1 cut(s) 229
MroXI GAANNNNTTC 2 cut(s) 867, 929
MseI TTAA 3 cut(s) 63, 396, 1014
MslI CAYNNNNRTG 1 cut(s) 944
MspA1I CMGCKG 1 cut(s) 1105
MspI CCGG 2 cut(s) 177, 1071
MspR9I CCNGG 4 cut(s) 178, 744, 805, 1355
MvaI CCWGG 3 cut(s) 744, 805, 1355
MwoI GCNNNNNNNGC 5 cut(s) 560, 701, 710, 959, 1219
NciI CCSGG 1 cut(s) 178
NcoI CCATGG 1 cut(s) 511
NdeI CATATG 2 cut(s) 1300, 1334
NdeII GATC 4 cut(s) 568, 955, 1073, 1378
NlaIV GGNNCC 1 cut(s) 440
NmuCI GTSAC 2 cut(s) 818, 1022
NsbI TGCGCA 2 cut(s) 297, 836
NsiI ATGCAT 1 cut(s) 229
NspI RCATGY 2 cut(s) 599, 1136
NspV TTCGAA 1 cut(s) 871
PaeI GCATGC 1 cut(s) 599
PagI TCATGA 1 cut(s) 1111
PceI AGGCCT 1 cut(s) 1213
PciI ACATGT 1 cut(s) 1132
PciSI GCTCTTC 1 cut(s) 1178
PcsI WCGNNNNNNNCGW 1 cut(s) 639
PdmI GAANNNNTTC 2 cut(s) 867, 929
PkrI GCNGC 4 cut(s) 706, 1104, 1107, 1372
PleI GAGTC 1 cut(s) 652
PpsI GAGTC 1 cut(s) 652
PscI ACATGT 1 cut(s) 1132
PsiI TTATAA 1 cut(s) 1272
Psp124BI GAGCTC 2 cut(s) 1066, 1190
Psp6I CCWGG 3 cut(s) 742, 803, 1353
PspEI GGTNACC 1 cut(s) 1348
PspFI CCCAGC 1 cut(s) 1343
PspGI CCWGG 3 cut(s) 742, 803, 1353
PspN4I GGNNCC 1 cut(s) 440
PspPI GGNCC 3 cut(s) 346, 476, 664
PstI CTGCAG 1 cut(s) 523
PstNI CAGNNNCTG 2 cut(s) 238, 1376
PsuI RGATCY 3 cut(s) 568, 955, 1073
PvuII CAGCTG 1 cut(s) 1105
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
RseI CAYNNNNRTG 1 cut(s) 944
SacI GAGCTC 2 cut(s) 1066, 1190
SapI GCTCTTC 1 cut(s) 1178
SaqAI TTAA 3 cut(s) 63, 396, 1014
SatI GCNGC 4 cut(s) 705, 1103, 1106, 1371
Sau3AI GATC 4 cut(s) 568, 955, 1073, 1378
Sau96I GGNCC 3 cut(s) 346, 476, 664
SchI GAGTC 1 cut(s) 653
ScrFI CCNGG 4 cut(s) 178, 744, 805, 1355
SduI GDGCHC 3 cut(s) 109, 1066, 1190
SfaNI GCATC 4 cut(s) 214, 525, 541, 1155
SfcI CTRYAG 5 cut(s) 199, 519, 1141, 1227, 1323
SfuI TTCGAA 1 cut(s) 871
SinI GGWCC 1 cut(s) 476
SmiMI CAYNNNNRTG 1 cut(s) 944
SmlI CTYRAG 1 cut(s) 1003
SmoI CTYRAG 1 cut(s) 1003
SphI GCATGC 1 cut(s) 599
Sse9I AATT 4 cut(s) 60, 171, 406, 1116
SseBI AGGCCT 1 cut(s) 1213
SsiI CCGC 1 cut(s) 702
SspI AATATT 1 cut(s) 688
SspMI CTAG 3 cut(s) 315, 540, 1408
SstI GAGCTC 2 cut(s) 1066, 1190
StuI AGGCCT 1 cut(s) 1213
StyD4I CCNGG 4 cut(s) 176, 742, 803, 1353
StyI CCWWGG 2 cut(s) 372, 511
TaaI ACNGT 2 cut(s) 1208, 1283
TaiI ACGT 3 cut(s) 633, 1024, 1197
TaqI TCGA 3 cut(s) 633, 871, 1096
TasI AATT 4 cut(s) 60, 171, 406, 1116
Tru1I TTAA 3 cut(s) 63, 396, 1014
Tru9I TTAA 3 cut(s) 63, 396, 1014
TscAI CASTG 2 cut(s) 109, 451
TseFI GTSAC 2 cut(s) 818, 1022
TseI GCWGC 4 cut(s) 704, 1102, 1105, 1370
Tsp45I GTSAC 2 cut(s) 818, 1022
TspDTI ATGAA 8 cut(s) 268, 484, 698, 876, 1100, 1128, 1217, 1322
TspRI CASTG 2 cut(s) 109, 451
VpaK11BI GGWCC 1 cut(s) 476
XagI CCTNNNNNAGG 1 cut(s) 318
XapI RAATTY 1 cut(s) 406
XceI RCATGY 2 cut(s) 599, 1136
XmiI GTMKAC 1 cut(s) 1233
XmnI GAANNNNTTC 2 cut(s) 867, 929
XspI CTAG 3 cut(s) 315, 540, 1408
Zsp2I ATGCAT 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.