Rw7G022620

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001085
Physical Location & Seq
Forward (+)
26672783 .. 26676064
3282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G022620.1

Sequence Viewer

Length: 1389 bp
ATGAGAGGGCAGTACTGTTCCAGTGGAGTATTTTTGGTATTGCTTACCATCTTTGGTGTGAGCGGTGCTCAACTTCCCAAGTTGATGCCACAAGAATTTCCAGAGGATTTCATTATTCATGTTGATAGGGGCTCTAGCAACAGTGACACAAATGGAACAAGATGGGCAGTTGTAGTTGCTGGGTCTATGGGTTACGGAAACTATAGGCATCAGGCTGATGTGTGTCATGCATATCAAATTTTGAAGAAAGGTGGACTGAAAGATGAAAACATCATTGTTTTCATGTACGATGACATTGCAAACAACCCTGAGAATCCAAGGCCGGGTGTCATCATCAACAATCCCAACGGCGATGACGTCTACCATGGAGTTCCCAAGGATTACACAGGAGAGAATGCTACTGCTGCTAATCTTTATGCTGTCATTCTTGGAGATAAATCTAATCTCTCTGGGGGTAGTGGCAAGGTTCTGAACAGCGGGCCAGACGACAATGTCTTTATCTATTACACCGACCATGGCGGGCCTGGGATCATTGGGATGCCTGAAGGCGATTATGTCTATGCCAATGATCTGGTAGATGTTCTGATAAAGAAGTATGAAGCTAATGCGTACAAAAACATGGTATTTTACCTTGAAGCTTGTGAATCCGGGAGTATGTTCGAGGGTCTACTGCCGACAAATATAAACATCTATGCAACCACAGCATCAAATGCAACTGAGAGTAGTTGGGGAACGTACTGCCCTGATATGACTCCTCCTCCACCTCCTGAATATGACACTTGCTTGGGTGATTTGTATAGTGTTTCTTGGATGGAGAATTGTGACTTATCAGATTTGCGCCAAGAGACTCTGGAAGAGCAATATCAATCGGTTAAATGGAGAACAGTCAATAATACTTATGGTTCTCATGTTATGCAATATGGAAATATGTATCAAACCTCAGATTTAGTATCCTTTTACTTGGGTGCAAATGATAACTCTTCCTCCATTGAAAGCAAATCGCCACAGTCAATCTTAGAAGTTGTTGACAATCGCGATGCAGATCTTCTTCATCTTTGGCACAAGTTCCAACGCGCACCCGCTGGCTCTGAAATGAAGCAGGAAGCTAAAAGGGTCCTAGATGATGAAATTTCAAGTAGGCAGCGCATTGACCACAACATAAACCAAATCGAACAACTTGTGTTGGGCTATTCAAGTAACTCTGTTCGTCCTGGGGGGCAAGCTCTTGTTGATGATTGGGACTGCTTCAAGACAGTTGTGAAAACTTATGAGAAACATTGTGGGTCATTGTCAAATTATGGTATGAAACACACACGAGTTTTTGCCAACATGTGCAATGCTGGGATCAGCGTGGAGCAAGTGGTTGCTGCATCAGCTCGAACTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

462

Amino Acids

51.11

Weight (kDa)

4.79

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 54 - 322 2.8e-115 Peptidase C13 family
Legum_prodom PF20985 373 - 462 5e-33 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
AatII GACGTC 1 cut(s) 360
AccBSI CCGCTC 1 cut(s) 63
AccI GTMKAC 2 cut(s) 360, 667
AccII CGCG 2 cut(s) 1035, 1074
AciI CCGC 4 cut(s) 63, 477, 519, 1080
AclWI GGATC 2 cut(s) 536, 1352
AcsI RAATTY 3 cut(s) 95, 237, 1128
AcuI CTGAAG 1 cut(s) 564
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 4 cut(s) 14, 287, 611, 737
AfiI CCNNNNNNNGG 1 cut(s) 323
AflIII ACRYGT 1 cut(s) 1329
AgsI TTSAA 6 cut(s) 244, 635, 992, 1134, 1194, 1249
AjnI CCWGG 2 cut(s) 523, 1210
AluBI AGCT 5 cut(s) 602, 638, 1106, 1223, 1376
AluI AGCT 5 cut(s) 602, 638, 1106, 1223, 1376
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 1 cut(s) 839
AlwI GGATC 2 cut(s) 536, 1352
AoxI GGCC 3 cut(s) 320, 479, 521
ApeKI GCWGC 3 cut(s) 404, 1141, 1367
ApoI RAATTY 3 cut(s) 95, 237, 1128
AspLEI GCGC 3 cut(s) 840, 1076, 1146
AspS9I GGNCC 3 cut(s) 479, 521, 1114
AsuC2I CCSGG 2 cut(s) 324, 649
AsuHPI GGTGA 1 cut(s) 800
AvaII GGWCC 1 cut(s) 1114
BanII GRGCYC 1 cut(s) 134
BauI CACGAG 1 cut(s) 1314
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 3 cut(s) 391, 1153, 1354
BccI CCATC 3 cut(s) 56, 156, 805
BceAI ACGGC 1 cut(s) 364
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 2 cut(s) 525, 1212
BciVI GTATCC 1 cut(s) 961
BcnI CCSGG 2 cut(s) 324, 649
BcoDI GTCTC 1 cut(s) 839
BfaI CTAG 3 cut(s) 135, 1118, 1387
BfmI CTRYAG 1 cut(s) 202
BfuI GTATCC 1 cut(s) 961
BglII AGATCT 1 cut(s) 1042
BisI GCNGC 3 cut(s) 405, 1142, 1368
BlsI GCNGC 3 cut(s) 406, 1143, 1369
BmcAI AGTACT 1 cut(s) 14
Bme1390I CCNGG 4 cut(s) 324, 525, 649, 1212
Bme18I GGWCC 1 cut(s) 1114
BmgT120I GGNCC 3 cut(s) 479, 521, 1114
BmiI GGNNCC 1 cut(s) 1115
BmrFI CCNGG 4 cut(s) 324, 525, 649, 1212
BmsI GCATC 6 cut(s) 75, 217, 528, 713, 1027, 1379
BplI GAGNNNNNCTC 2 cut(s) 52, 84
BpuMI CCSGG 2 cut(s) 324, 649
BsaBI GATNNNNATC 1 cut(s) 1041
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 6 cut(s) 317, 364, 375, 514, 524, 1211
BsaXI ACNNNNNCTCC 4 cut(s) 742, 772, 968, 998
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse1I ACTGG 1 cut(s) 21
Bse3DI GCAATG 2 cut(s) 294, 1342
Bse8I GATNNNNATC 1 cut(s) 1041
BseBI CCWGG 2 cut(s) 525, 1212
BseDI CCNNGG 6 cut(s) 317, 364, 375, 514, 524, 1211
BseGI GGATG 2 cut(s) 543, 816
BseJI GATNNNNATC 1 cut(s) 1041
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMI GCAATG 2 cut(s) 294, 1342
BseMII CTCAG 3 cut(s) 300, 708, 954
BseNI ACTGG 1 cut(s) 21
BseRI GAGGAG 2 cut(s) 744, 747
BseXI GCAGC 3 cut(s) 391, 1153, 1354
BseYI CCCAGC 2 cut(s) 179, 1340
Bsh1236I CGCG 2 cut(s) 1035, 1074
BshFI GGCC 3 cut(s) 322, 481, 523
BsiHKAI GWGCWC 1 cut(s) 70
BsiSI CCGG 2 cut(s) 323, 648
BslFI GGGAC 1 cut(s) 1253
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 1 cut(s) 839
BsmFI GGGAC 1 cut(s) 1253
BsmI GAATGC 1 cut(s) 400
BsnI GGCC 3 cut(s) 322, 481, 523
Bsp1286I GDGCHC 2 cut(s) 70, 134
Bsp143I GATC 4 cut(s) 528, 568, 1042, 1344
Bsp19I CCATGG 2 cut(s) 364, 514
Bsp68I TCGCGA 1 cut(s) 1035
BspACI CCGC 4 cut(s) 63, 477, 519, 1080
BspANI GGCC 3 cut(s) 322, 481, 523
BspCNI CTCAG 3 cut(s) 301, 709, 953
BspFNI CGCG 2 cut(s) 1035, 1074
BspLI GGNNCC 1 cut(s) 1115
BspPI GGATC 2 cut(s) 536, 1352
BspQI GCTCTTC 1 cut(s) 849
BsrBI CCGCTC 1 cut(s) 63
BsrDI GCAATG 2 cut(s) 294, 1342
BsrI ACTGG 1 cut(s) 21
BssECI CCNNGG 6 cut(s) 317, 364, 375, 514, 524, 1211
BssMI GATC 4 cut(s) 528, 568, 1042, 1344
BssNI GRCGYC 1 cut(s) 357
BssSI CACGAG 1 cut(s) 1314
BssT1I CCWWGG 4 cut(s) 317, 364, 375, 514
Bst2BI CACGAG 1 cut(s) 1314
Bst2UI CCWGG 2 cut(s) 525, 1212
Bst4CI ACNGT 5 cut(s) 17, 143, 886, 1008, 1255
Bst6I CTCTTC 2 cut(s) 849, 985
BstACI GRCGYC 1 cut(s) 357
BstAPI GCANNNNNTGC 1 cut(s) 710
BstC8I GCNNGC 4 cut(s) 479, 521, 1084, 1221
BstDEI CTNAG 4 cut(s) 309, 717, 940, 1015
BstDSI CCRYGG 2 cut(s) 364, 514
BstF5I GGATG 2 cut(s) 543, 816
BstFNI CGCG 2 cut(s) 1035, 1074
BstHHI GCGC 3 cut(s) 840, 1076, 1146
BstKTI GATC 4 cut(s) 531, 571, 1045, 1347
BstMAI GTCTC 1 cut(s) 839
BstMBI GATC 4 cut(s) 528, 568, 1042, 1344
BstMWI GCNNNNNNNGC 4 cut(s) 404, 701, 710, 1373
BstNI CCWGG 2 cut(s) 525, 1212
BstNSI RCATGY 1 cut(s) 1333
BstSCI CCNGG 4 cut(s) 322, 523, 647, 1210
BstSFI CTRYAG 1 cut(s) 202
BstUI CGCG 2 cut(s) 1035, 1074
BstV1I GCAGC 3 cut(s) 391, 1153, 1354
BstX2I RGATCY 1 cut(s) 1042
BstXI CCANNNNNNTGG 1 cut(s) 571
BstYI RGATCY 1 cut(s) 1042
BsuI GTATCC 1 cut(s) 961
BsuRI GGCC 3 cut(s) 322, 481, 523
BtgI CCRYGG 2 cut(s) 364, 514
BtgZI GCGATG 2 cut(s) 366, 1050
BtsCI GGATG 2 cut(s) 543, 816
BtsIMutI CAGTG 2 cut(s) 28, 148
BtuMI TCGCGA 1 cut(s) 1035
Cac8I GCNNGC 4 cut(s) 479, 521, 1084, 1221
CfoI GCGC 3 cut(s) 840, 1076, 1146
Cfr13I GGNCC 3 cut(s) 479, 521, 1114
Csp6I GTAC 4 cut(s) 13, 286, 610, 736
CviAII CATG 8 cut(s) 119, 227, 283, 365, 515, 619, 908, 1330
CviQI GTAC 4 cut(s) 13, 286, 610, 736
DdeI CTNAG 4 cut(s) 309, 717, 940, 1015
DpnI GATC 4 cut(s) 530, 570, 1044, 1346
DpnII GATC 4 cut(s) 528, 568, 1042, 1344
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
Eam1104I CTCTTC 2 cut(s) 849, 985
EarI CTCTTC 2 cut(s) 849, 985
Eco130I CCWWGG 4 cut(s) 317, 364, 375, 514
Eco24I GRGCYC 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 1114
Eco57I CTGAAG 1 cut(s) 564
EcoO109I RGGNCCY 1 cut(s) 1114
EcoRII CCWGG 2 cut(s) 523, 1210
EcoT14I CCWWGG 4 cut(s) 317, 364, 375, 514
EcoT22I ATGCAT 1 cut(s) 232
EcoT38I GRGCYC 1 cut(s) 134
ErhI CCWWGG 4 cut(s) 317, 364, 375, 514
FaeI CATG 8 cut(s) 122, 230, 286, 368, 518, 622, 911, 1333
FaqI GGGAC 1 cut(s) 1253
FatI CATG 8 cut(s) 118, 226, 282, 364, 514, 618, 907, 1329
FauI CCCGC 3 cut(s) 470, 512, 1087
FblI GTMKAC 2 cut(s) 360, 667
Fnu4HI GCNGC 3 cut(s) 405, 1142, 1368
FokI GGATG 2 cut(s) 550, 823
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 3 cut(s) 405, 1142, 1368
FspBI CTAG 3 cut(s) 135, 1118, 1387
GlaI GCGC 3 cut(s) 839, 1075, 1145
GluI GCNGC 3 cut(s) 405, 1142, 1368
GsaI CCCAGC 2 cut(s) 183, 1344
HaeIII GGCC 3 cut(s) 322, 481, 523
HapII CCGG 2 cut(s) 323, 648
HhaI GCGC 3 cut(s) 840, 1076, 1146
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 8 cut(s) 122, 230, 286, 368, 518, 622, 911, 1333
Hin6I GCGC 3 cut(s) 838, 1074, 1144
HinP1I GCGC 3 cut(s) 838, 1074, 1144
HincII GTYRAC 1 cut(s) 1027
HindII GTYRAC 1 cut(s) 1027
HindIII AAGCTT 1 cut(s) 636
HinfI GANTC 4 cut(s) 313, 644, 751, 847
HpaII CCGG 2 cut(s) 323, 648
HphI GGTGA 1 cut(s) 800
Hpy166II GTNNAC 4 cut(s) 254, 361, 668, 1027
Hpy188I TCNGA 5 cut(s) 471, 585, 832, 943, 1090
Hpy188III TCNNGA 5 cut(s) 101, 767, 851, 1034, 1249
Hpy8I GTNNAC 4 cut(s) 254, 361, 668, 1027
HpyAV CCTTC 1 cut(s) 539
HpyCH4III ACNGT 5 cut(s) 17, 143, 886, 1008, 1255
HpyCH4IV ACGT 2 cut(s) 357, 734
HpyCH4V TGCA 9 cut(s) 230, 299, 695, 713, 916, 968, 1040, 1335, 1370
HpyF10VI GCNNNNNNNGC 4 cut(s) 404, 701, 710, 1373
HpyF3I CTNAG 4 cut(s) 309, 717, 940, 1015
HpySE526I ACGT 2 cut(s) 357, 734
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 8 cut(s) 122, 230, 286, 368, 518, 622, 911, 1333
HspAI GCGC 3 cut(s) 838, 1074, 1144
Kzo9I GATC 4 cut(s) 528, 568, 1042, 1344
LguI GCTCTTC 1 cut(s) 849
LmnI GCTCC 1 cut(s) 1354
Lsp1109I GCAGC 3 cut(s) 391, 1153, 1354
LweI GCATC 6 cut(s) 75, 217, 528, 713, 1027, 1379
MaeI CTAG 3 cut(s) 135, 1118, 1387
MaeII ACGT 2 cut(s) 357, 734
MaeIII GTNAC 4 cut(s) 143, 191, 821, 1196
MalI GATC 4 cut(s) 530, 570, 1044, 1346
MbiI CCGCTC 1 cut(s) 63
MboI GATC 4 cut(s) 528, 568, 1042, 1344
MboII GAAGA 5 cut(s) 256, 866, 972, 1037, 1040
MflI RGATCY 1 cut(s) 1042
MhlI GDGCHC 2 cut(s) 70, 134
MluCI AATT 5 cut(s) 95, 237, 817, 1128, 1294
MlyI GAGTC 2 cut(s) 745, 841
MmeI TCCRAC 1 cut(s) 1093
MnlI CCTC 7 cut(s) 97, 655, 765, 768, 774, 949, 994
Mph1103I ATGCAT 1 cut(s) 232
MseI TTAA 1 cut(s) 873
MslI CAYNNNNRTG 1 cut(s) 536
MspA1I CMGCKG 2 cut(s) 477, 1082
MspI CCGG 2 cut(s) 323, 648
MspR9I CCNGG 4 cut(s) 324, 525, 649, 1212
Mva1269I GAATGC 1 cut(s) 400
MvaI CCWGG 2 cut(s) 525, 1212
MvnI CGCG 2 cut(s) 1035, 1074
MwoI GCNNNNNNNGC 4 cut(s) 404, 701, 710, 1373
NciI CCSGG 2 cut(s) 324, 649
NcoI CCATGG 2 cut(s) 364, 514
NdeII GATC 4 cut(s) 528, 568, 1042, 1344
NlaIII CATG 8 cut(s) 122, 230, 286, 368, 518, 622, 911, 1333
NlaIV GGNNCC 1 cut(s) 1115
NmuCI GTSAC 2 cut(s) 143, 821
NruI TCGCGA 1 cut(s) 1035
NsiI ATGCAT 1 cut(s) 232
NspI RCATGY 1 cut(s) 1333
PciI ACATGT 1 cut(s) 1329
PciSI GCTCTTC 1 cut(s) 849
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PctI GAATGC 1 cut(s) 400
PfeI GAWTC 2 cut(s) 313, 644
PflFI GACNNNGTC 1 cut(s) 491
PfoI TCCNGGA 1 cut(s) 647
PkrI GCNGC 3 cut(s) 406, 1143, 1369
PleI GAGTC 2 cut(s) 745, 841
PpsI GAGTC 2 cut(s) 745, 841
PpuMI RGGWCCY 1 cut(s) 1114
PscI ACATGT 1 cut(s) 1329
Psp5II RGGWCCY 1 cut(s) 1114
Psp6I CCWGG 2 cut(s) 523, 1210
PspFI CCCAGC 2 cut(s) 179, 1340
PspGI CCWGG 2 cut(s) 523, 1210
PspN4I GGNNCC 1 cut(s) 1115
PspPI GGNCC 3 cut(s) 479, 521, 1114
PspPPI RGGWCCY 1 cut(s) 1114
PsuI RGATCY 1 cut(s) 1042
PsyI GACNNNGTC 1 cut(s) 491
RruI TCGCGA 1 cut(s) 1035
RsaI GTAC 4 cut(s) 14, 287, 611, 737
RsaNI GTAC 4 cut(s) 13, 286, 610, 736
RseI CAYNNNNRTG 1 cut(s) 536
SapI GCTCTTC 1 cut(s) 849
SaqAI TTAA 1 cut(s) 873
SatI GCNGC 3 cut(s) 405, 1142, 1368
Sau3AI GATC 4 cut(s) 528, 568, 1042, 1344
Sau96I GGNCC 3 cut(s) 479, 521, 1114
ScaI AGTACT 1 cut(s) 14
SchI GAGTC 2 cut(s) 745, 841
ScrFI CCNGG 4 cut(s) 324, 525, 649, 1212
SduI GDGCHC 2 cut(s) 70, 134
SfaNI GCATC 6 cut(s) 75, 217, 528, 713, 1027, 1379
SfcI CTRYAG 1 cut(s) 202
SinI GGWCC 1 cut(s) 1114
SmiMI CAYNNNNRTG 1 cut(s) 536
Sse9I AATT 5 cut(s) 95, 237, 817, 1128, 1294
SsiI CCGC 4 cut(s) 63, 477, 519, 1080
SspMI CTAG 3 cut(s) 135, 1118, 1387
StyD4I CCNGG 4 cut(s) 322, 523, 647, 1210
StyI CCWWGG 4 cut(s) 317, 364, 375, 514
TaaI ACNGT 5 cut(s) 17, 143, 886, 1008, 1255
TaiI ACGT 2 cut(s) 360, 737
TaqI TCGA 3 cut(s) 660, 1170, 1378
TasI AATT 5 cut(s) 95, 237, 817, 1128, 1294
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 2 cut(s) 313, 644
Tru1I TTAA 1 cut(s) 873
Tru9I TTAA 1 cut(s) 873
TscAI CASTG 2 cut(s) 28, 148
TseFI GTSAC 2 cut(s) 143, 821
TseI GCWGC 3 cut(s) 404, 1141, 1367
Tsp45I GTSAC 2 cut(s) 143, 821
TspDTI ATGAA 9 cut(s) 100, 107, 271, 279, 612, 1040, 1109, 1140, 1319
TspGWI ACGGA 1 cut(s) 210
TspRI CASTG 2 cut(s) 28, 148
Tth111I GACNNNGTC 1 cut(s) 491
VpaK11BI GGWCC 1 cut(s) 1114
XapI RAATTY 3 cut(s) 95, 237, 1128
XceI RCATGY 1 cut(s) 1333
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XmiI GTMKAC 2 cut(s) 360, 667
XspI CTAG 3 cut(s) 135, 1118, 1387
ZraI GACGTC 1 cut(s) 358
ZrmI AGTACT 1 cut(s) 14
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.