Rmu_sc0008839.1_g000015

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008839.1
OG0001085
Physical Location & Seq
Reverse (-)
44817 .. 47666
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008839.1_g000015.1.cds

Sequence Viewer

Length: 984 bp
atgaaagggcagtactgttccagtggagtatttttggtattgcttaccatctttggtgtgagcggtgctcaacttcccaagttgatgccacaagaatttccagaggatttcattattcatgttgataggggctctagcaacagtgacacaaatgaaacaagatgggcagttgtagttgctgggtctatgggttacggaaactataggcatcaggctgatgtgtgtcatgcatatcaaattttgaagaaaggtggactgaaagatgaaaacatcattgttttcatgtacgatgacattgcaaacaaccctgagaatccaaggccgggtgtcatcatcaacaatcccaacggcgatgacgtctaccatggagttcccaaggattacacaggagagaatgctactgctgctaatctttatgctgtcattcttggagataaatctaatctctctgggggtagtggcaaggttctgaacagcgggccagacgacaatgtctttatctattacaccgaccatggcgggcctgggatcattgggatgcctgaaggcgattatgtctatgccaatgatctggtagatgttctgataaagaagtatgaagctaatgcgtacaaaaacatggtattttaccttgaagcttgtgaatccgggagtatgttcgagggtctactgccgacaaatataaacatctatgcaaccacagcatcaaatgcaactgagagtagttggggaacgcagcgcattgaccacaacataaaccaaatcgaacaacttgtgttgggctattcaagtaactctgttcgtcctggggggcaagctcttgttgatgattgggactgcttcaagacagttgtgaaaacttatgagaaacattgtgggtcattgtcaaattatggtatgaaacacacacgagtttttgccaacatgtgcaatgctgggatcagcgtggagcaagtggttgctgcatcagctcgaacttgctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

327

Amino Acids

35.64

Weight (kDa)

4.97

Isoelectric Point (pI)

30.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
AatII GACGTC 1 cut(s) 360
AccBSI CCGCTC 1 cut(s) 63
AccI GTMKAC 2 cut(s) 360, 667
AciI CCGC 3 cut(s) 63, 477, 519
AclWI GGATC 2 cut(s) 536, 947
AcsI RAATTY 2 cut(s) 95, 237
AcuI CTGAAG 1 cut(s) 564
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 3 cut(s) 14, 287, 611
AfiI CCNNNNNNNGG 1 cut(s) 323
AflIII ACRYGT 1 cut(s) 924
AgsI TTSAA 4 cut(s) 244, 635, 789, 844
AjnI CCWGG 2 cut(s) 523, 805
AluBI AGCT 4 cut(s) 602, 638, 818, 971
AluI AGCT 4 cut(s) 602, 638, 818, 971
Alw21I GWGCWC 1 cut(s) 70
AlwI GGATC 2 cut(s) 536, 947
AoxI GGCC 3 cut(s) 320, 479, 521
ApeKI GCWGC 3 cut(s) 404, 736, 962
ApoI RAATTY 2 cut(s) 95, 237
AspLEI GCGC 1 cut(s) 741
AspS9I GGNCC 2 cut(s) 479, 521
AsuC2I CCSGG 2 cut(s) 324, 649
BanII GRGCYC 1 cut(s) 134
BauI CACGAG 1 cut(s) 909
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 3 cut(s) 391, 748, 949
BccI CCATC 2 cut(s) 56, 156
BceAI ACGGC 1 cut(s) 364
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 2 cut(s) 525, 807
BcnI CCSGG 2 cut(s) 324, 649
BfaI CTAG 2 cut(s) 135, 982
BfmI CTRYAG 1 cut(s) 202
BisI GCNGC 3 cut(s) 405, 737, 963
BlsI GCNGC 3 cut(s) 406, 738, 964
BmcAI AGTACT 1 cut(s) 14
Bme1390I CCNGG 4 cut(s) 324, 525, 649, 807
BmgT120I GGNCC 2 cut(s) 479, 521
BmrFI CCNGG 4 cut(s) 324, 525, 649, 807
BmsI GCATC 5 cut(s) 75, 217, 528, 713, 974
BplI GAGNNNNNCTC 2 cut(s) 52, 84
BpuMI CCSGG 2 cut(s) 324, 649
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 6 cut(s) 317, 364, 375, 514, 524, 806
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse1I ACTGG 1 cut(s) 21
Bse3DI GCAATG 2 cut(s) 294, 937
BseBI CCWGG 2 cut(s) 525, 807
BseDI CCNNGG 6 cut(s) 317, 364, 375, 514, 524, 806
BseGI GGATG 1 cut(s) 543
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMI GCAATG 2 cut(s) 294, 937
BseMII CTCAG 2 cut(s) 300, 708
BseNI ACTGG 1 cut(s) 21
BseXI GCAGC 3 cut(s) 391, 748, 949
BseYI CCCAGC 2 cut(s) 179, 935
BshFI GGCC 3 cut(s) 322, 481, 523
BsiHKAI GWGCWC 1 cut(s) 70
BsiSI CCGG 2 cut(s) 323, 648
BslFI GGGAC 1 cut(s) 848
BslI CCNNNNNNNGG 1 cut(s) 323
BsmFI GGGAC 1 cut(s) 848
BsmI GAATGC 1 cut(s) 400
BsnI GGCC 3 cut(s) 322, 481, 523
Bsp1286I GDGCHC 2 cut(s) 70, 134
Bsp143I GATC 3 cut(s) 528, 568, 939
Bsp19I CCATGG 2 cut(s) 364, 514
BspACI CCGC 3 cut(s) 63, 477, 519
BspANI GGCC 3 cut(s) 322, 481, 523
BspCNI CTCAG 2 cut(s) 301, 709
BspPI GGATC 2 cut(s) 536, 947
BsrBI CCGCTC 1 cut(s) 63
BsrDI GCAATG 2 cut(s) 294, 937
BsrI ACTGG 1 cut(s) 21
BssECI CCNNGG 6 cut(s) 317, 364, 375, 514, 524, 806
BssMI GATC 3 cut(s) 528, 568, 939
BssNI GRCGYC 1 cut(s) 357
BssSI CACGAG 1 cut(s) 909
BssT1I CCWWGG 4 cut(s) 317, 364, 375, 514
Bst2BI CACGAG 1 cut(s) 909
Bst2UI CCWGG 2 cut(s) 525, 807
Bst4CI ACNGT 3 cut(s) 17, 143, 850
BstACI GRCGYC 1 cut(s) 357
BstAPI GCANNNNNTGC 1 cut(s) 710
BstC8I GCNNGC 3 cut(s) 479, 521, 816
BstDEI CTNAG 2 cut(s) 309, 717
BstDSI CCRYGG 2 cut(s) 364, 514
BstF5I GGATG 1 cut(s) 543
BstHHI GCGC 1 cut(s) 741
BstKTI GATC 3 cut(s) 531, 571, 942
BstMBI GATC 3 cut(s) 528, 568, 939
BstMWI GCNNNNNNNGC 4 cut(s) 404, 701, 710, 968
BstNI CCWGG 2 cut(s) 525, 807
BstNSI RCATGY 1 cut(s) 928
BstSCI CCNGG 4 cut(s) 322, 523, 647, 805
BstSFI CTRYAG 1 cut(s) 202
BstV1I GCAGC 3 cut(s) 391, 748, 949
BstXI CCANNNNNNTGG 1 cut(s) 571
BsuRI GGCC 3 cut(s) 322, 481, 523
BtgI CCRYGG 2 cut(s) 364, 514
BtgZI GCGATG 1 cut(s) 366
BtsCI GGATG 1 cut(s) 543
BtsIMutI CAGTG 2 cut(s) 28, 148
Cac8I GCNNGC 3 cut(s) 479, 521, 816
CfoI GCGC 1 cut(s) 741
Cfr13I GGNCC 2 cut(s) 479, 521
Csp6I GTAC 3 cut(s) 13, 286, 610
CviAII CATG 7 cut(s) 119, 227, 283, 365, 515, 619, 925
CviQI GTAC 3 cut(s) 13, 286, 610
DdeI CTNAG 2 cut(s) 309, 717
DpnI GATC 3 cut(s) 530, 570, 941
DpnII GATC 3 cut(s) 528, 568, 939
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
Eco130I CCWWGG 4 cut(s) 317, 364, 375, 514
Eco24I GRGCYC 1 cut(s) 134
Eco57I CTGAAG 1 cut(s) 564
EcoRII CCWGG 2 cut(s) 523, 805
EcoT14I CCWWGG 4 cut(s) 317, 364, 375, 514
EcoT22I ATGCAT 1 cut(s) 232
EcoT38I GRGCYC 1 cut(s) 134
ErhI CCWWGG 4 cut(s) 317, 364, 375, 514
FaeI CATG 7 cut(s) 122, 230, 286, 368, 518, 622, 928
FaqI GGGAC 1 cut(s) 848
FatI CATG 7 cut(s) 118, 226, 282, 364, 514, 618, 924
FauI CCCGC 2 cut(s) 470, 512
FblI GTMKAC 2 cut(s) 360, 667
Fnu4HI GCNGC 3 cut(s) 405, 737, 963
FokI GGATG 1 cut(s) 550
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 3 cut(s) 405, 737, 963
FspBI CTAG 2 cut(s) 135, 982
GlaI GCGC 1 cut(s) 740
GluI GCNGC 3 cut(s) 405, 737, 963
GsaI CCCAGC 2 cut(s) 183, 939
HaeIII GGCC 3 cut(s) 322, 481, 523
HapII CCGG 2 cut(s) 323, 648
HhaI GCGC 1 cut(s) 741
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 7 cut(s) 122, 230, 286, 368, 518, 622, 928
Hin6I GCGC 1 cut(s) 739
HinP1I GCGC 1 cut(s) 739
HindIII AAGCTT 1 cut(s) 636
HinfI GANTC 2 cut(s) 313, 644
HpaII CCGG 2 cut(s) 323, 648
Hpy166II GTNNAC 3 cut(s) 254, 361, 668
Hpy188I TCNGA 2 cut(s) 471, 585
Hpy188III TCNNGA 2 cut(s) 101, 844
Hpy8I GTNNAC 3 cut(s) 254, 361, 668
HpyAV CCTTC 1 cut(s) 539
HpyCH4III ACNGT 3 cut(s) 17, 143, 850
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 6 cut(s) 230, 299, 695, 713, 930, 965
HpyF10VI GCNNNNNNNGC 4 cut(s) 404, 701, 710, 968
HpyF3I CTNAG 2 cut(s) 309, 717
HpySE526I ACGT 1 cut(s) 357
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 7 cut(s) 122, 230, 286, 368, 518, 622, 928
HspAI GCGC 1 cut(s) 739
Kzo9I GATC 3 cut(s) 528, 568, 939
LmnI GCTCC 1 cut(s) 949
Lsp1109I GCAGC 3 cut(s) 391, 748, 949
LweI GCATC 5 cut(s) 75, 217, 528, 713, 974
MaeI CTAG 2 cut(s) 135, 982
MaeII ACGT 1 cut(s) 357
MaeIII GTNAC 3 cut(s) 143, 191, 791
MalI GATC 3 cut(s) 530, 570, 941
MbiI CCGCTC 1 cut(s) 63
MboI GATC 3 cut(s) 528, 568, 939
MboII GAAGA 1 cut(s) 256
MhlI GDGCHC 2 cut(s) 70, 134
MluCI AATT 3 cut(s) 95, 237, 889
MnlI CCTC 2 cut(s) 97, 655
Mph1103I ATGCAT 1 cut(s) 232
MslI CAYNNNNRTG 1 cut(s) 536
MspA1I CMGCKG 1 cut(s) 477
MspI CCGG 2 cut(s) 323, 648
MspR9I CCNGG 4 cut(s) 324, 525, 649, 807
Mva1269I GAATGC 1 cut(s) 400
MvaI CCWGG 2 cut(s) 525, 807
MwoI GCNNNNNNNGC 4 cut(s) 404, 701, 710, 968
NciI CCSGG 2 cut(s) 324, 649
NcoI CCATGG 2 cut(s) 364, 514
NdeII GATC 3 cut(s) 528, 568, 939
NlaIII CATG 7 cut(s) 122, 230, 286, 368, 518, 622, 928
NmuCI GTSAC 1 cut(s) 143
NsiI ATGCAT 1 cut(s) 232
NspI RCATGY 1 cut(s) 928
PciI ACATGT 1 cut(s) 924
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PctI GAATGC 1 cut(s) 400
PfeI GAWTC 2 cut(s) 313, 644
PflFI GACNNNGTC 1 cut(s) 491
PfoI TCCNGGA 1 cut(s) 647
PkrI GCNGC 3 cut(s) 406, 738, 964
PscI ACATGT 1 cut(s) 924
Psp6I CCWGG 2 cut(s) 523, 805
PspFI CCCAGC 2 cut(s) 179, 935
PspGI CCWGG 2 cut(s) 523, 805
PspPI GGNCC 2 cut(s) 479, 521
PsyI GACNNNGTC 1 cut(s) 491
RsaI GTAC 3 cut(s) 14, 287, 611
RsaNI GTAC 3 cut(s) 13, 286, 610
RseI CAYNNNNRTG 1 cut(s) 536
SatI GCNGC 3 cut(s) 405, 737, 963
Sau3AI GATC 3 cut(s) 528, 568, 939
Sau96I GGNCC 2 cut(s) 479, 521
ScaI AGTACT 1 cut(s) 14
ScrFI CCNGG 4 cut(s) 324, 525, 649, 807
SduI GDGCHC 2 cut(s) 70, 134
SetI ASST 8 cut(s) 253, 360, 468, 604, 633, 640, 820, 973
SfaNI GCATC 5 cut(s) 75, 217, 528, 713, 974
SfcI CTRYAG 1 cut(s) 202
SmiMI CAYNNNNRTG 1 cut(s) 536
Sse9I AATT 3 cut(s) 95, 237, 889
SsiI CCGC 3 cut(s) 63, 477, 519
SspMI CTAG 2 cut(s) 135, 982
StyD4I CCNGG 4 cut(s) 322, 523, 647, 805
StyI CCWWGG 4 cut(s) 317, 364, 375, 514
TaaI ACNGT 3 cut(s) 17, 143, 850
TaiI ACGT 1 cut(s) 360
TaqI TCGA 3 cut(s) 660, 765, 973
TasI AATT 3 cut(s) 95, 237, 889
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 2 cut(s) 313, 644
TscAI CASTG 2 cut(s) 28, 148
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 3 cut(s) 404, 736, 962
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 8 cut(s) 17, 100, 107, 168, 271, 279, 612, 914
TspGWI ACGGA 1 cut(s) 210
TspRI CASTG 2 cut(s) 28, 148
Tth111I GACNNNGTC 1 cut(s) 491
XapI RAATTY 2 cut(s) 95, 237
XceI RCATGY 1 cut(s) 928
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XmiI GTMKAC 2 cut(s) 360, 667
XspI CTAG 2 cut(s) 135, 982
ZraI GACGTC 1 cut(s) 358
ZrmI AGTACT 1 cut(s) 14
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.