MD17G1264800.v1.1

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001085
Physical Location & Seq
Reverse (-)
32519005 .. 32525520
6516 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1264800.v1.1.491

Sequence Viewer

Length: 972 bp
ATGAATGATGAAAATATCATTGTTTTCATGTATGATGATATTACATTGGCCGAGGATAATCCCAAATCTGGTGTCATCGTCAACAAGCCAGACGGCAAGGATGTTTATAAAGGAGTTCCAAAGGATTATACAAAGGATGATGTAAAGGCAGGGAATTTGTATGCAGTTATTCTTGGGAACAAAAGTGCCCTCAGTGGAGGTAATTTACCTGGATGTAGTCCAAACAGCATAAATATTTACGCTATGGCTGCAGCAAATGCAGAAGAGAGTAGTTACGCAACTTACTGCCCTGGCCAAGTTCATGTTCCTCCAGAATATCACACCTGCTTGGGAGACTTGTACAGTATATCCTGGATGGAGGATTGCAGCGATGCAGAAGATTTGCAGACAGAAACTTTGGCCAAGCAATATGAACTGGTTCGAAAAAGAACAAATGCATCTCATGTTATGGAGTATGGAGATATGAGTCATAGGCAGGATGTCCTCTCTACTTACATGGGTGGAGCTCCTGCAGCAAACCCTAGCTATACTTCCACTTCCATCAAAGACAACTCCACATCGAGCTCAAGGATTGTTAGCCAACTCGACGCCGACCTCGTTTACTTCCTGCACAAGTTCCATGGAGCTCCAACTGGTTCTCAGAAGAAGCTGGAAGCTCGGAAGCAGCTGCTCGATGAAATTGCTCAAAGGGAGCATGCGGACTACAGCGTAAAGAAAATTGGCGAGCTTTTGTTTGGGAATGAGATGGCCTCCAAGGTGTTGATGAATGTCAGGCCTCCGGGGCAACGGTTGGTAGATGATTGGGACTGCTTTAAGACGCTTTTGGGAACTTATGGGAGGCGTTGTGAAGGTTTATCAAGATATGGAAGGAAATACCCAAGGGCAATTGCCAACATATGCAATGCTGGGACCAGCATGGAGAAAATGGATGCAGCCTCACATGAAGCTTGTTCTAAGAAACCCCATCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

324

Amino Acids

35.63

Weight (kDa)

5.87

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 2 - 67 4.2e-20 Peptidase C13 family
Peptidase_C13 PF01650 65 - 167 4.9e-29 Peptidase C13 family
Legum_prodom PF20985 221 - 317 2.3e-33 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 108
AarI CACCTGC 1 cut(s) 332
Acc36I ACCTGC 1 cut(s) 332
AciI CCGC 1 cut(s) 698
AcoI YGGCCR 3 cut(s) 48, 292, 399
AcsI RAATTY 1 cut(s) 154
AcyI GRCGYC 1 cut(s) 588
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 1 cut(s) 68
AjnI CCWGG 3 cut(s) 208, 289, 350
AjuI GAANNNNNNNTTGG 2 cut(s) 288, 320
AluBI AGCT 9 cut(s) 506, 525, 564, 626, 649, 656, 667, 727, 947
AluI AGCT 9 cut(s) 506, 525, 564, 626, 649, 656, 667, 727, 947
Alw21I GWGCWC 3 cut(s) 508, 566, 628
Alw26I GTCTC 1 cut(s) 327
AoxI GGCC 5 cut(s) 48, 292, 399, 747, 773
ApeKI GCWGC 7 cut(s) 248, 251, 366, 512, 664, 667, 932
ApoI RAATTY 1 cut(s) 154
ArsI GACNNNNNNTTYG 4 cut(s) 57, 89, 379, 411
Asp700I GAANNNNTTC 1 cut(s) 417
AspS9I GGNCC 1 cut(s) 909
AsuC2I CCSGG 1 cut(s) 780
AsuII TTCGAA 1 cut(s) 421
AvaII GGWCC 1 cut(s) 909
BaeGI GKGCMC 1 cut(s) 190
BalI TGGCCA 2 cut(s) 294, 401
BanII GRGCYC 3 cut(s) 508, 566, 628
Bbv12I GWGCWC 3 cut(s) 508, 566, 628
BbvI GCAGC 7 cut(s) 235, 263, 378, 524, 654, 676, 944
BccI CCATC 3 cut(s) 349, 548, 739
BceAI ACGGC 1 cut(s) 109
BcgI CGANNNNNNTGC 2 cut(s) 662, 696
BciT130I CCWGG 3 cut(s) 210, 291, 352
BcnI CCSGG 1 cut(s) 780
BcoDI GTCTC 1 cut(s) 327
BfaI CTAG 2 cut(s) 522, 970
BfmI CTRYAG 3 cut(s) 249, 510, 703
BfuAI ACCTGC 1 cut(s) 332
BglI GCCNNNNNGGC 1 cut(s) 781
BisI GCNGC 7 cut(s) 249, 252, 367, 513, 665, 668, 933
BlsI GCNGC 7 cut(s) 250, 253, 368, 514, 666, 669, 934
Bme1390I CCNGG 4 cut(s) 210, 291, 352, 780
Bme18I GGWCC 1 cut(s) 909
BmgT120I GGNCC 1 cut(s) 909
BmiI GGNNCC 1 cut(s) 910
BmrFI CCNGG 4 cut(s) 210, 291, 352, 780
BmsI GCATC 3 cut(s) 361, 446, 919
BplI GAGNNNNNCTC 2 cut(s) 734, 766
BpmI CTGGAG 1 cut(s) 294
Bpu14I TTCGAA 1 cut(s) 421
BpuEI CTTGAG 1 cut(s) 550
BpuMI CCSGG 1 cut(s) 780
BsaHI GRCGYC 1 cut(s) 588
BsaJI CCNNGG 6 cut(s) 51, 289, 619, 753, 779, 878
BsaXI ACNNNNNCTCC 2 cut(s) 829, 859
Bsc4I CCNNNNNNNGG 1 cut(s) 68
Bse1I ACTGG 2 cut(s) 420, 637
Bse3DI GCAATG 1 cut(s) 907
BseBI CCWGG 3 cut(s) 210, 291, 352
BseDI CCNNGG 6 cut(s) 51, 289, 619, 753, 779, 878
BseGI GGATG 6 cut(s) 106, 142, 218, 360, 484, 934
BseLI CCNNNNNNNGG 1 cut(s) 68
BseMI GCAATG 1 cut(s) 907
BseMII CTCAG 2 cut(s) 205, 653
BseNI ACTGG 2 cut(s) 420, 637
BseSI GKGCMC 1 cut(s) 190
BseXI GCAGC 7 cut(s) 235, 263, 378, 524, 654, 676, 944
BseYI CCCAGC 1 cut(s) 905
BsgI GTGCAG 1 cut(s) 593
BshFI GGCC 5 cut(s) 50, 294, 401, 749, 775
BsiHKAI GWGCWC 3 cut(s) 508, 566, 628
BsiSI CCGG 1 cut(s) 779
BslFI GGGAC 2 cut(s) 818, 922
BslI CCNNNNNNNGG 1 cut(s) 68
BsmAI GTCTC 1 cut(s) 327
BsmFI GGGAC 2 cut(s) 818, 922
BsnI GGCC 5 cut(s) 50, 294, 401, 749, 775
Bsp119I TTCGAA 1 cut(s) 421
Bsp1286I GDGCHC 4 cut(s) 190, 508, 566, 628
Bsp1407I TGTACA 1 cut(s) 339
Bsp19I CCATGG 1 cut(s) 619
BspACI CCGC 1 cut(s) 698
BspANI GGCC 5 cut(s) 50, 294, 401, 749, 775
BspCNI CTCAG 2 cut(s) 204, 652
BspLI GGNNCC 1 cut(s) 910
BspMAI CTGCAG 2 cut(s) 253, 514
BspMI ACCTGC 1 cut(s) 332
BspT104I TTCGAA 1 cut(s) 421
BsrDI GCAATG 1 cut(s) 907
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 2 cut(s) 420, 637
BssECI CCNNGG 6 cut(s) 51, 289, 619, 753, 779, 878
BssNI GRCGYC 1 cut(s) 588
BssT1I CCWWGG 3 cut(s) 619, 753, 878
Bst2UI CCWGG 3 cut(s) 210, 291, 352
Bst4CI ACNGT 2 cut(s) 344, 789
Bst6I CTCTTC 1 cut(s) 258
BstACI GRCGYC 1 cut(s) 588
BstAPI GCANNNNNTGC 1 cut(s) 257
BstAUI TGTACA 1 cut(s) 339
BstBI TTCGAA 1 cut(s) 421
BstC8I GCNNGC 2 cut(s) 696, 725
BstDEI CTNAG 3 cut(s) 191, 639, 954
BstDSI CCRYGG 1 cut(s) 619
BstF5I GGATG 6 cut(s) 106, 142, 218, 360, 484, 934
BstMAI GTCTC 1 cut(s) 327
BstMWI GCNNNNNNNGC 4 cut(s) 248, 257, 512, 781
BstNI CCWGG 3 cut(s) 210, 291, 352
BstNSI RCATGY 1 cut(s) 698
BstSCI CCNGG 4 cut(s) 208, 289, 350, 778
BstSFI CTRYAG 3 cut(s) 249, 510, 703
BstSLI GKGCMC 1 cut(s) 190
BstV1I GCAGC 7 cut(s) 235, 263, 378, 524, 654, 676, 944
BsuRI GGCC 5 cut(s) 50, 294, 401, 749, 775
BtgI CCRYGG 1 cut(s) 619
BtgZI GCGATG 1 cut(s) 384
BtsCI GGATG 6 cut(s) 106, 142, 218, 360, 484, 934
BtsIMutI CAGTG 1 cut(s) 199
BveI ACCTGC 1 cut(s) 332
Cac8I GCNNGC 2 cut(s) 696, 725
Cfr13I GGNCC 1 cut(s) 909
CseI GACGC 2 cut(s) 596, 826
Csp6I GTAC 1 cut(s) 340
CviAII CATG 8 cut(s) 28, 302, 443, 496, 620, 695, 916, 941
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 3 cut(s) 191, 639, 954
EaeI YGGCCR 3 cut(s) 48, 292, 399
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Ecl136II GAGCTC 3 cut(s) 506, 564, 626
Eco130I CCWWGG 3 cut(s) 619, 753, 878
Eco147I AGGCCT 1 cut(s) 775
Eco24I GRGCYC 3 cut(s) 508, 566, 628
Eco47I GGWCC 1 cut(s) 909
Eco53kI GAGCTC 3 cut(s) 506, 564, 626
EcoICRI GAGCTC 3 cut(s) 506, 564, 626
EcoRII CCWGG 3 cut(s) 208, 289, 350
EcoT14I CCWWGG 3 cut(s) 619, 753, 878
EcoT22I ATGCAT 1 cut(s) 439
EcoT38I GRGCYC 3 cut(s) 508, 566, 628
ErhI CCWWGG 3 cut(s) 619, 753, 878
FaeI CATG 8 cut(s) 31, 305, 446, 499, 623, 698, 919, 944
FaqI GGGAC 2 cut(s) 818, 922
FatI CATG 8 cut(s) 27, 301, 442, 495, 619, 694, 915, 940
FauNDI CATATG 1 cut(s) 896
Fnu4HI GCNGC 7 cut(s) 249, 252, 367, 513, 665, 668, 933
FokI GGATG 6 cut(s) 113, 149, 225, 367, 491, 941
FriOI GRGCYC 3 cut(s) 508, 566, 628
Fsp4HI GCNGC 7 cut(s) 249, 252, 367, 513, 665, 668, 933
FspBI CTAG 2 cut(s) 522, 970
GluI GCNGC 7 cut(s) 249, 252, 367, 513, 665, 668, 933
GsaI CCCAGC 1 cut(s) 909
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 5 cut(s) 50, 294, 401, 749, 775
HapII CCGG 1 cut(s) 779
HgaI GACGC 2 cut(s) 596, 826
Hin1I GRCGYC 1 cut(s) 588
Hin1II CATG 8 cut(s) 31, 305, 446, 499, 623, 698, 919, 944
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 945
HinfI GANTC 1 cut(s) 466
HpaII CCGG 1 cut(s) 779
Hpy166II GTNNAC 2 cut(s) 82, 601
Hpy188I TCNGA 2 cut(s) 642, 660
Hpy188III TCNNGA 2 cut(s) 311, 858
Hpy8I GTNNAC 2 cut(s) 82, 601
Hpy99I CGWCG 1 cut(s) 590
HpyAV CCTTC 2 cut(s) 842, 861
HpyCH4III ACNGT 2 cut(s) 344, 789
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 257, 512, 781
HpyF3I CTNAG 3 cut(s) 191, 639, 954
Hsp92I GRCGYC 1 cut(s) 588
Hsp92II CATG 8 cut(s) 31, 305, 446, 499, 623, 698, 919, 944
LmnI GCTCC 5 cut(s) 503, 511, 623, 631, 691
Lsp1109I GCAGC 7 cut(s) 235, 263, 378, 524, 654, 676, 944
LweI GCATC 3 cut(s) 361, 446, 919
MaeI CTAG 2 cut(s) 522, 970
MaeIII GTNAC 1 cut(s) 272
MboII GAAGA 3 cut(s) 275, 389, 655
MfeI CAATTG 1 cut(s) 885
MhlI GDGCHC 4 cut(s) 190, 508, 566, 628
MlsI TGGCCA 2 cut(s) 294, 401
MluCI AATT 5 cut(s) 154, 202, 678, 717, 885
MluNI TGGCCA 2 cut(s) 294, 401
MlyI GAGTC 1 cut(s) 475
MmeI TCCRAC 1 cut(s) 653
Mox20I TGGCCA 2 cut(s) 294, 401
Mph1103I ATGCAT 1 cut(s) 439
MroXI GAANNNNTTC 1 cut(s) 417
MscI TGGCCA 2 cut(s) 294, 401
MseI TTAA 1 cut(s) 813
Msp20I TGGCCA 2 cut(s) 294, 401
MspA1I CMGCKG 1 cut(s) 667
MspI CCGG 1 cut(s) 779
MspR9I CCNGG 4 cut(s) 210, 291, 352, 780
MunI CAATTG 1 cut(s) 885
MvaI CCWGG 3 cut(s) 210, 291, 352
MwoI GCNNNNNNNGC 4 cut(s) 248, 257, 512, 781
NciI CCSGG 1 cut(s) 780
NcoI CCATGG 1 cut(s) 619
NdeI CATATG 1 cut(s) 896
NlaIII CATG 8 cut(s) 31, 305, 446, 499, 623, 698, 919, 944
NlaIV GGNNCC 1 cut(s) 910
NmeAIII GCCGAG 1 cut(s) 76
NsiI ATGCAT 1 cut(s) 439
NspI RCATGY 1 cut(s) 698
NspV TTCGAA 1 cut(s) 421
PaeI GCATGC 1 cut(s) 698
PaqCI CACCTGC 1 cut(s) 332
PceI AGGCCT 1 cut(s) 775
PcsI WCGNNNNNNNCGW 1 cut(s) 594
PdmI GAANNNNTTC 1 cut(s) 417
PfoI TCCNGGA 1 cut(s) 350
PkrI GCNGC 7 cut(s) 250, 253, 368, 514, 666, 669, 934
PleI GAGTC 1 cut(s) 474
PpsI GAGTC 1 cut(s) 474
PsiI TTATAA 1 cut(s) 108
Psp124BI GAGCTC 3 cut(s) 508, 566, 628
Psp6I CCWGG 3 cut(s) 208, 289, 350
PspFI CCCAGC 1 cut(s) 905
PspGI CCWGG 3 cut(s) 208, 289, 350
PspN4I GGNNCC 1 cut(s) 910
PspPI GGNCC 1 cut(s) 909
PstI CTGCAG 2 cut(s) 253, 514
PvuII CAGCTG 1 cut(s) 667
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
SacI GAGCTC 3 cut(s) 508, 566, 628
SaqAI TTAA 1 cut(s) 813
SatI GCNGC 7 cut(s) 249, 252, 367, 513, 665, 668, 933
Sau96I GGNCC 1 cut(s) 909
SchI GAGTC 1 cut(s) 475
ScrFI CCNGG 4 cut(s) 210, 291, 352, 780
SduI GDGCHC 4 cut(s) 190, 508, 566, 628
SfaNI GCATC 3 cut(s) 361, 446, 919
SfcI CTRYAG 3 cut(s) 249, 510, 703
SfuI TTCGAA 1 cut(s) 421
SinI GGWCC 1 cut(s) 909
SmlI CTYRAG 1 cut(s) 565
SmoI CTYRAG 1 cut(s) 565
SphI GCATGC 1 cut(s) 698
Sse9I AATT 5 cut(s) 154, 202, 678, 717, 885
SseBI AGGCCT 1 cut(s) 775
SsiI CCGC 1 cut(s) 698
SspI AATATT 1 cut(s) 235
SspMI CTAG 2 cut(s) 522, 970
SstI GAGCTC 3 cut(s) 508, 566, 628
StuI AGGCCT 1 cut(s) 775
StyD4I CCNGG 4 cut(s) 208, 289, 350, 778
StyI CCWWGG 3 cut(s) 619, 753, 878
TaaI ACNGT 2 cut(s) 344, 789
TaqI TCGA 4 cut(s) 421, 560, 585, 672
TasI AATT 5 cut(s) 154, 202, 678, 717, 885
TatI WGTACW 1 cut(s) 339
Tru1I TTAA 1 cut(s) 813
Tru9I TTAA 1 cut(s) 813
TscAI CASTG 1 cut(s) 199
TseI GCWGC 7 cut(s) 248, 251, 366, 512, 664, 667, 932
TspDTI ATGAA 8 cut(s) 16, 17, 24, 290, 426, 690, 779, 957
TspRI CASTG 1 cut(s) 199
VpaK11BI GGWCC 1 cut(s) 909
XapI RAATTY 1 cut(s) 154
XceI RCATGY 1 cut(s) 698
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 2 cut(s) 522, 970
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.