RLG00000020368

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001085
Physical Location & Seq
Reverse (-)
64283534 .. 64287881
4348 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020368

Sequence Viewer

Length: 1020 bp
ATGAGTATTCAAGGTTTAGTAGTACTTCTTTTTCTGTCTGTTTTGAGTGTGAGTACTATTGGGAGCACTCTTGTTGAAAATGGTTCTACGAGTACCACTCATCATAAGTCCAAGGGAAAAAGATGGGCACTTCTAATTGCTGGTTCAAATGGTTACGATAACTATCGACACCAGGCTGATGTATGCCATGCATACCAGATACTTAAGAAAGGTGGACTCAAAGATAAAAACATTATTGTCTTCATGTATGATGATATTGCCAACAACACAGAAAACCCTAGACCTGGTGTCATTATCAACAAGCCAAACGGCAAGGATGTCTACCACGGAGTTCCCAAGGATTACACAGGAGATGATGTTAACTCCCACAATTTCTACGCTGCTATTCTCGGAGACAAAACTGCAGTCAAAGGAGGAAGTGGCAAGGTTCTGGAGACTGGTCCAAATGACCATATTTTCATATATTACACTGATCATGGTGCTGCAGGACTTCTTGCTTGTGAGTCTGGGAGTATGTTTGAGGGTTTGCTTCCAAAGAATATGAACATTTATGCTACCACAGCATCAAATGCAGAGGAGAGTAGTTATGCAACATACTGTGATGGTGATCCTGGTGTTCCTGAAGAATTCGGAGGACTGAAAGATGAGAACATCATCGTTTTCATGTACGACGATATTGCATCAGACAGAGAAAATTCTAGGCCTGGTGTCATTATCAACAATCCAAATGGTATTAATGTTTACAACGGAGTTCCCAAGGATTATACATTAGGTGATGTTATTACAATCAATATTTTCGCTGTTATTCTCGGAAACAAAAGTGCCCTCATTGGAGAAGGTGGCAAAGTATTCTACCTTGAAGCTGCATTTGGAGGGAGTCTATTTGAGGGTCTCGTGCCAGATAGAATGAGTATCTATGCTACTACAGCATCACGAGAAAGTTTTGCAGCATGTCAACATGATCCTGCTGTTCCTGCAGAATATACCACCTATTTGGGAGACTTGTATATGACAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

340

Amino Acids

36.58

Weight (kDa)

5.0

Isoelectric Point (pI)

41.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 41 - 165 8.2e-60 Peptidase C13 family
Peptidase_C13 PF01650 166 - 202 2.9e-13 Peptidase C13 family
Peptidase_C13 PF01650 211 - 283 1.1e-20 Peptidase C13 family
Peptidase_C13 PF01650 283 - 337 1.1e-07 Peptidase C13 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 321
AclWI GGATC 2 cut(s) 602, 956
AcsI RAATTY 2 cut(s) 626, 694
AcuI CTGAAG 1 cut(s) 642
AfaI GTAC 4 cut(s) 24, 55, 94, 668
AfiI CCNNNNNNNGG 1 cut(s) 284
AflII CTTAAG 1 cut(s) 203
AgsI TTSAA 4 cut(s) 11, 77, 147, 860
AhdI GACNNNNNGTC 1 cut(s) 287
AjnI CCWGG 4 cut(s) 171, 283, 610, 703
AjuI GAANNNNNNNTTGG 2 cut(s) 852, 884
AluBI AGCT 1 cut(s) 863
AluI AGCT 1 cut(s) 863
Alw21I GWGCWC 1 cut(s) 68
Alw26I GTCTC 4 cut(s) 387, 428, 896, 993
AlwI GGATC 2 cut(s) 602, 956
AoxI GGCC 1 cut(s) 701
ApeKI GCWGC 4 cut(s) 380, 482, 863, 947
ApoI RAATTY 2 cut(s) 626, 694
ArsI GACNNNNNNTTYG 2 cut(s) 390, 422
AseI ATTAAT 1 cut(s) 735
AspS9I GGNCC 1 cut(s) 440
AsuHPI GGTGA 2 cut(s) 617, 785
AvaII GGWCC 1 cut(s) 440
BaeGI GKGCMC 2 cut(s) 130, 826
BauI CACGAG 2 cut(s) 893, 933
BbsI GAAGAC 1 cut(s) 232
Bbv12I GWGCWC 1 cut(s) 68
BbvI GCAGC 4 cut(s) 367, 469, 850, 959
BccI CCATC 2 cut(s) 117, 596
BceAI ACGGC 1 cut(s) 325
BcgI CGANNNNNNTGC 2 cut(s) 659, 693
BciT130I CCWGG 4 cut(s) 173, 285, 612, 705
BclI TGATCA 1 cut(s) 472
BcoDI GTCTC 4 cut(s) 387, 428, 896, 993
BfaI CTAG 2 cut(s) 279, 699
BfmI CTRYAG 4 cut(s) 402, 483, 924, 975
BfrI CTTAAG 1 cut(s) 203
BisI GCNGC 4 cut(s) 381, 483, 864, 948
BlsI GCNGC 4 cut(s) 382, 484, 865, 949
BmcAI AGTACT 2 cut(s) 24, 55
Bme1390I CCNGG 4 cut(s) 173, 285, 612, 705
Bme18I GGWCC 1 cut(s) 440
BmeRI GACNNNNNGTC 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 440
BmrFI CCNGG 4 cut(s) 173, 285, 612, 705
BmsI GCATC 3 cut(s) 572, 689, 938
BpiI GAAGAC 1 cut(s) 232
BplI GAGNNNNNCTC 2 cut(s) 82, 114
BpmI CTGGAG 1 cut(s) 452
BsaBI GATNNNNATC 2 cut(s) 162, 606
BsaI GGTCTC 1 cut(s) 896
BsaJI CCNNGG 4 cut(s) 111, 325, 336, 756
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 442
Bse8I GATNNNNATC 2 cut(s) 162, 606
BseBI CCWGG 4 cut(s) 173, 285, 612, 705
BseDI CCNNGG 4 cut(s) 111, 325, 336, 756
BseGI GGATG 1 cut(s) 322
BseJI GATNNNNATC 2 cut(s) 162, 606
BseLI CCNNNNNNNGG 1 cut(s) 284
BseNI ACTGG 1 cut(s) 442
BseRI GAGGAG 1 cut(s) 590
BseSI GKGCMC 2 cut(s) 130, 826
BseXI GCAGC 4 cut(s) 367, 469, 850, 959
BshFI GGCC 1 cut(s) 703
BsiHKAI GWGCWC 1 cut(s) 68
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 4 cut(s) 387, 428, 896, 993
BsnI GGCC 1 cut(s) 703
Bso31I GGTCTC 1 cut(s) 896
Bsp1286I GDGCHC 3 cut(s) 68, 130, 826
Bsp143I GATC 3 cut(s) 472, 607, 961
BspANI GGCC 1 cut(s) 703
BspMAI CTGCAG 3 cut(s) 406, 487, 979
BspPI GGATC 2 cut(s) 602, 956
BspTI CTTAAG 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 896
BsrI ACTGG 1 cut(s) 442
BssECI CCNNGG 4 cut(s) 111, 325, 336, 756
BssMI GATC 3 cut(s) 472, 607, 961
BssSI CACGAG 2 cut(s) 893, 933
BssT1I CCWWGG 3 cut(s) 111, 336, 756
Bst2BI CACGAG 2 cut(s) 893, 933
Bst2UI CCWGG 4 cut(s) 173, 285, 612, 705
Bst4CI ACNGT 1 cut(s) 599
BstAFI CTTAAG 1 cut(s) 203
BstAPI GCANNNNNTGC 1 cut(s) 569
BstDSI CCRYGG 1 cut(s) 325
BstF5I GGATG 1 cut(s) 322
BstKTI GATC 3 cut(s) 475, 610, 964
BstMAI GTCTC 4 cut(s) 387, 428, 896, 993
BstMBI GATC 3 cut(s) 472, 607, 961
BstMWI GCNNNNNNNGC 4 cut(s) 560, 569, 926, 974
BstNI CCWGG 4 cut(s) 173, 285, 612, 705
BstNSI RCATGY 1 cut(s) 954
BstSCI CCNGG 4 cut(s) 171, 283, 610, 703
BstSFI CTRYAG 4 cut(s) 402, 483, 924, 975
BstSLI GKGCMC 2 cut(s) 130, 826
BstV1I GCAGC 4 cut(s) 367, 469, 850, 959
BstV2I GAAGAC 1 cut(s) 232
BstXI CCANNNNNNTGG 1 cut(s) 994
BsuRI GGCC 1 cut(s) 703
BtgI CCRYGG 1 cut(s) 325
BtsCI GGATG 1 cut(s) 322
BtsIMutI CAGTG 1 cut(s) 468
Cfr13I GGNCC 1 cut(s) 440
CsiI ACCWGGT 1 cut(s) 283
Csp6I GTAC 4 cut(s) 23, 54, 93, 667
CviAII CATG 6 cut(s) 188, 244, 476, 664, 951, 959
CviJI RGCY 4 cut(s) 176, 304, 703, 863
CviKI_1 RGCY 4 cut(s) 176, 304, 703, 863
CviQI GTAC 4 cut(s) 23, 54, 93, 667
DpnI GATC 3 cut(s) 474, 609, 963
DpnII GATC 3 cut(s) 472, 607, 961
DriI GACNNNNNGTC 1 cut(s) 287
Eam1105I GACNNNNNGTC 1 cut(s) 287
Eco130I CCWWGG 3 cut(s) 111, 336, 756
Eco147I AGGCCT 1 cut(s) 703
Eco31I GGTCTC 1 cut(s) 896
Eco47I GGWCC 1 cut(s) 440
Eco57I CTGAAG 1 cut(s) 642
EcoRI GAATTC 1 cut(s) 626
EcoRII CCWGG 4 cut(s) 171, 283, 610, 703
EcoT14I CCWWGG 3 cut(s) 111, 336, 756
EcoT22I ATGCAT 1 cut(s) 193
ErhI CCWWGG 3 cut(s) 111, 336, 756
FaeI CATG 6 cut(s) 191, 247, 479, 667, 954, 962
FatI CATG 6 cut(s) 187, 243, 475, 663, 950, 958
FbaI TGATCA 1 cut(s) 472
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 4 cut(s) 381, 483, 864, 948
FokI GGATG 1 cut(s) 329
Fsp4HI GCNGC 4 cut(s) 381, 483, 864, 948
FspBI CTAG 2 cut(s) 279, 699
GluI GCNGC 4 cut(s) 381, 483, 864, 948
GsuI CTGGAG 1 cut(s) 452
HaeIII GGCC 1 cut(s) 703
Hin1II CATG 6 cut(s) 191, 247, 479, 667, 954, 962
HincII GTYRAC 2 cut(s) 361, 956
HindII GTYRAC 2 cut(s) 361, 956
HinfI GANTC 3 cut(s) 216, 503, 877
HpaI GTTAAC 1 cut(s) 361
HphI GGTGA 2 cut(s) 617, 785
Hpy166II GTNNAC 5 cut(s) 215, 322, 361, 742, 956
Hpy188I TCNGA 4 cut(s) 392, 632, 685, 812
Hpy188III TCNNGA 3 cut(s) 431, 620, 933
Hpy8I GTNNAC 5 cut(s) 215, 322, 361, 742, 956
Hpy99I CGWCG 1 cut(s) 674
HpyAV CCTTC 1 cut(s) 830
HpyCH4III ACNGT 1 cut(s) 599
HpyCH4V TGCA 9 cut(s) 191, 404, 485, 572, 590, 680, 866, 947, 977
HpyF10VI GCNNNNNNNGC 4 cut(s) 560, 569, 926, 974
Hsp92II CATG 6 cut(s) 191, 247, 479, 667, 954, 962
Ksp22I TGATCA 1 cut(s) 472
KspAI GTTAAC 1 cut(s) 361
Kzo9I GATC 3 cut(s) 472, 607, 961
LmnI GCTCC 1 cut(s) 63
Lsp1109I GCAGC 4 cut(s) 367, 469, 850, 959
LweI GCATC 3 cut(s) 572, 689, 938
MabI ACCWGGT 1 cut(s) 283
MaeI CTAG 2 cut(s) 279, 699
MaeIII GTNAC 1 cut(s) 152
MalI GATC 3 cut(s) 474, 609, 963
MboI GATC 3 cut(s) 472, 607, 961
MboII GAAGA 2 cut(s) 232, 635
MhlI GDGCHC 3 cut(s) 68, 130, 826
MluCI AATT 4 cut(s) 135, 370, 626, 694
MlyI GAGTC 3 cut(s) 210, 512, 886
MnlI CCTC 7 cut(s) 407, 514, 568, 626, 836, 866, 880
Mph1103I ATGCAT 1 cut(s) 193
MseI TTAA 3 cut(s) 204, 360, 735
MspCI CTTAAG 1 cut(s) 203
MspR9I CCNGG 4 cut(s) 173, 285, 612, 705
MvaI CCWGG 4 cut(s) 173, 285, 612, 705
MwoI GCNNNNNNNGC 4 cut(s) 560, 569, 926, 974
NdeII GATC 3 cut(s) 472, 607, 961
NlaIII CATG 6 cut(s) 191, 247, 479, 667, 954, 962
NsiI ATGCAT 1 cut(s) 193
NspI RCATGY 1 cut(s) 954
PceI AGGCCT 1 cut(s) 703
PkrI GCNGC 4 cut(s) 382, 484, 865, 949
PleI GAGTC 3 cut(s) 210, 511, 885
PpsI GAGTC 3 cut(s) 210, 511, 885
PshBI ATTAAT 1 cut(s) 735
Psp6I CCWGG 4 cut(s) 171, 283, 610, 703
PspGI CCWGG 4 cut(s) 171, 283, 610, 703
PspPI GGNCC 1 cut(s) 440
PstI CTGCAG 3 cut(s) 406, 487, 979
RsaI GTAC 4 cut(s) 24, 55, 94, 668
RsaNI GTAC 4 cut(s) 23, 54, 93, 667
SaqAI TTAA 3 cut(s) 204, 360, 735
SatI GCNGC 4 cut(s) 381, 483, 864, 948
Sau3AI GATC 3 cut(s) 472, 607, 961
Sau96I GGNCC 1 cut(s) 440
ScaI AGTACT 2 cut(s) 24, 55
SchI GAGTC 3 cut(s) 210, 512, 886
ScrFI CCNGG 4 cut(s) 173, 285, 612, 705
SduI GDGCHC 3 cut(s) 68, 130, 826
SetI ASST 9 cut(s) 16, 214, 286, 429, 775, 841, 858, 865, 992
SexAI ACCWGGT 1 cut(s) 283
SfaNI GCATC 3 cut(s) 572, 689, 938
SfcI CTRYAG 4 cut(s) 402, 483, 924, 975
SinI GGWCC 1 cut(s) 440
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 4 cut(s) 135, 370, 626, 694
SseBI AGGCCT 1 cut(s) 703
SspI AATATT 1 cut(s) 793
SspMI CTAG 2 cut(s) 279, 699
StuI AGGCCT 1 cut(s) 703
StyD4I CCNGG 4 cut(s) 171, 283, 610, 703
StyI CCWWGG 3 cut(s) 111, 336, 756
TaaI ACNGT 1 cut(s) 599
TaqI TCGA 1 cut(s) 166
TasI AATT 4 cut(s) 135, 370, 626, 694
TatI WGTACW 2 cut(s) 22, 53
Tru1I TTAA 3 cut(s) 204, 360, 735
Tru9I TTAA 3 cut(s) 204, 360, 735
TscAI CASTG 1 cut(s) 475
TseI GCWGC 4 cut(s) 380, 482, 863, 947
TspDTI ATGAA 4 cut(s) 232, 448, 557, 652
TspGWI ACGGA 2 cut(s) 342, 762
TspRI CASTG 1 cut(s) 475
Vha464I CTTAAG 1 cut(s) 203
VpaK11BI GGWCC 1 cut(s) 440
VspI ATTAAT 1 cut(s) 735
XapI RAATTY 2 cut(s) 626, 694
XceI RCATGY 1 cut(s) 954
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 2 cut(s) 279, 699
ZrmI AGTACT 2 cut(s) 24, 55
Zsp2I ATGCAT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.