Rh7BG330500

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001085
Physical Location & Seq
Reverse (-)
34159530 .. 34166531
7002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG330500.1

Sequence Viewer

Length: 1437 bp
ATGTTCTTTTGTTATGTAGTTGAATTGGTCACTATGATCCTAATCACAAGGCCTCGCTATGTTTTACTACTATTCCTTACATTACTGAGTTTGACATCCATTTCACCATCATCATCAAAAGTGGAAGATATCAGCAACGGAACAAGATGGGCAGTTCTGATTGCTGGGTCAAGAGGATATGATAACTATAGGCATCAGGCTGATGTATGCCATGCATACCAGATACTGAAGAAAGGTGGACTCAAAGATGAGAATATCATTGTGTTCATGTATGACGATATTGCATTCAACCCAAAAAATCCTAGACTTGGTGTCATTATCAACAAGCCAGATGGAAATGATGTTTATCAAGGAGTTCCCAAGGACTATACAGGAAAGGATATTAATACTCACAACTTCTATGCTGTTCTTCTTGGCAACATAAGTGCCCTCACTGGTGGTAGCGGCAACGGCAAGGTTCTAAATAGCGGTCCAAACGACCGCGTTTTCATTTATTACGCTGACCATGGCAGCCCAGGGATGGTTGGCATGCCAATTGAAGGTGATTACGTATATGCTAAAGATCTCATAGATGTGCTGAAGAAGAAGCACGATGCAAAAGGTTACAAAAGCATGGTGTTTTACATGGAAGCTTGCGAGTCTGGGAGCCTGTTTGAGGGCCTCCTTCCCAACAATATAAGCATATACGCAACTACAGCAGCAAATTCAAAAGAGAGCAGTTGGGGATTCTATTGTCCTGGAGACTTCCCTAGTCCTCCTCCAGGATATGACGTTTGCTTGGGAGACTTGTATAGTATTTCTTGGCTTGAGGATTGTGACAAGCATGACTTGAGCAAAGAAACTTTGGCGAAGCAATATGAAGTGGTCCGAAGGAGAACAGCAAATGGCTACTTAGATTCAAGCCATGTCATGCAATTTGGGAATAAGAGACAAAGCGAGGATTTCATCTTCACTTACATGGGTACAAATCCTGAAAATGATAACTATACTTCCTTCAGGTACACGGCATCACCAGCGACCTCAAGGCCAAGGGTTGTCAGCCAACGCAACGCAGACCTCCTCCATCTTCGGTACAAGTTCGACAATGCCCCTGATGGCTCCGATGTGAAGCTTGAAGCTCACAAACAGCTCATTGATGAAATTTCTCGGAGGAAAAAGGTGGATCATAACATAAAGCAGATAGGGAAGCTTTTGTTTGGGTATGACAAGAGTTCAGAAGTGATGAAGAATGTTAGGACTCCGGGGGAACCTCTAGTAGATGATTGGGACTGCTTTAAGATGCTTGTGAAAACTTATGAGAAACACTGTGGAATTTTATCAAGTTATGGAAGGAAACACACGAGAGCTATTGCCAACATGTGCAATACTGGAGTTACGCCGGAACAAATGGCTGCTGCATGTGTTCAAACCTGTCATATGAAATCCCCTCTCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

478

Amino Acids

53.64

Weight (kDa)

7.55

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 49 - 322 1.3e-112 Peptidase C13 family
Legum_prodom PF20985 375 - 471 7e-39 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 483
AciI CCGC 3 cut(s) 444, 468, 481
AclWI GGATC 2 cut(s) 31, 1170
AcsI RAATTY 3 cut(s) 703, 1140, 1311
AcuI CTGAAG 3 cut(s) 248, 599, 979
AfaI GTAC 3 cut(s) 964, 1001, 1073
AfiI CCNNNNNNNGG 5 cut(s) 308, 520, 539, 655, 761
AflIII ACRYGT 1 cut(s) 1356
AgsI TTSAA 7 cut(s) 23, 289, 539, 708, 900, 1115, 1406
AhdI GACNNNNNGTC 1 cut(s) 311
AjnI CCWGG 3 cut(s) 514, 736, 760
AluBI AGCT 6 cut(s) 632, 1111, 1118, 1129, 1189, 1346
AluI AGCT 6 cut(s) 632, 1111, 1118, 1129, 1189, 1346
Alw26I GTCTC 3 cut(s) 735, 777, 922
AlwI GGATC 2 cut(s) 31, 1170
AlwNI CAGNNNCTG 1 cut(s) 226
AoxI GGCC 3 cut(s) 50, 658, 1025
ApeKI GCWGC 4 cut(s) 510, 698, 1391, 1394
ApoI RAATTY 3 cut(s) 703, 1140, 1311
AseI ATTAAT 1 cut(s) 384
AspS9I GGNCC 3 cut(s) 470, 658, 865
AsuC2I CCSGG 1 cut(s) 1242
AsuHPI GGTGA 3 cut(s) 96, 554, 1002
AvaII GGWCC 2 cut(s) 470, 865
BaeGI GKGCMC 1 cut(s) 430
BauI CACGAG 1 cut(s) 1339
BbvI GCAGC 4 cut(s) 522, 710, 1378, 1381
BccI CCATC 6 cut(s) 115, 141, 326, 514, 1071, 1088
BceAI ACGGC 2 cut(s) 466, 1020
BciT130I CCWGG 3 cut(s) 516, 738, 762
BcnI CCSGG 1 cut(s) 1242
BcoDI GTCTC 3 cut(s) 735, 777, 922
BfaI CTAG 3 cut(s) 303, 750, 1253
BfmI CTRYAG 2 cut(s) 187, 693
BglII AGATCT 1 cut(s) 562
BisI GCNGC 5 cut(s) 445, 511, 699, 1392, 1395
BlsI GCNGC 5 cut(s) 446, 512, 700, 1393, 1396
Bme1390I CCNGG 4 cut(s) 516, 738, 762, 1242
Bme18I GGWCC 2 cut(s) 470, 865
BmeRI GACNNNNNGTC 1 cut(s) 311
BmgT120I GGNCC 3 cut(s) 470, 658, 865
BmiI GGNNCC 3 cut(s) 647, 1099, 1248
BmrFI CCNGG 4 cut(s) 516, 738, 762, 1242
BmsI GCATC 4 cut(s) 202, 583, 1016, 1269
BpmI CTGGAG 3 cut(s) 744, 759, 1389
BpuEI CTTGAG 3 cut(s) 827, 850, 1006
BpuMI CCSGG 1 cut(s) 1242
BsaAI YACGTR 1 cut(s) 550
BsaBI GATNNNNATC 2 cut(s) 41, 345
BsaJI CCNNGG 6 cut(s) 360, 505, 514, 515, 1028, 1241
Bsc4I CCNNNNNNNGG 5 cut(s) 308, 520, 539, 655, 761
Bse1I ACTGG 2 cut(s) 439, 1372
Bse8I GATNNNNATC 2 cut(s) 41, 345
BseBI CCWGG 3 cut(s) 516, 738, 762
BseDI CCNNGG 6 cut(s) 360, 505, 514, 515, 1028, 1241
BseGI GGATG 2 cut(s) 95, 525
BseJI GATNNNNATC 2 cut(s) 41, 345
BseLI CCNNNNNNNGG 5 cut(s) 308, 520, 539, 655, 761
BseMII CTCAG 1 cut(s) 77
BseNI ACTGG 2 cut(s) 439, 1372
BseRI GAGGAG 2 cut(s) 747, 1049
BseSI GKGCMC 1 cut(s) 430
BseXI GCAGC 4 cut(s) 522, 710, 1378, 1381
BseYI CCCAGC 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 483
Bsh1285I CGRYCG 1 cut(s) 481
BshFI GGCC 3 cut(s) 52, 660, 1027
BsiEI CGRYCG 1 cut(s) 481
BsiSI CCGG 2 cut(s) 1241, 1379
BslFI GGGAC 1 cut(s) 1280
BslI CCNNNNNNNGG 5 cut(s) 308, 520, 539, 655, 761
BsmAI GTCTC 3 cut(s) 735, 777, 922
BsmFI GGGAC 1 cut(s) 1280
BsmI GAATGC 1 cut(s) 284
BsnI GGCC 3 cut(s) 52, 660, 1027
Bsp1286I GDGCHC 1 cut(s) 430
Bsp143I GATC 3 cut(s) 36, 562, 1162
Bsp19I CCATGG 1 cut(s) 505
BspACI CCGC 3 cut(s) 444, 468, 481
BspANI GGCC 3 cut(s) 52, 660, 1027
BspCNI CTCAG 1 cut(s) 78
BspFNI CGCG 1 cut(s) 483
BspLI GGNNCC 3 cut(s) 647, 1099, 1248
BspPI GGATC 2 cut(s) 31, 1170
BsrI ACTGG 2 cut(s) 439, 1372
BssECI CCNNGG 6 cut(s) 360, 505, 514, 515, 1028, 1241
BssMI GATC 3 cut(s) 36, 562, 1162
BssSI CACGAG 1 cut(s) 1339
BssT1I CCWWGG 3 cut(s) 360, 505, 1028
Bst2BI CACGAG 1 cut(s) 1339
Bst2UI CCWGG 3 cut(s) 516, 738, 762
Bst4CI ACNGT 1 cut(s) 1307
BstBAI YACGTR 1 cut(s) 550
BstC8I GCNNGC 2 cut(s) 530, 634
BstDEI CTNAG 2 cut(s) 86, 892
BstDSI CCRYGG 1 cut(s) 505
BstENI CCTNNNNNAGG 2 cut(s) 653, 759
BstF5I GGATG 2 cut(s) 95, 525
BstFNI CGCG 1 cut(s) 483
BstKTI GATC 3 cut(s) 39, 565, 1165
BstMAI GTCTC 3 cut(s) 735, 777, 922
BstMBI GATC 3 cut(s) 36, 562, 1162
BstMCI CGRYCG 1 cut(s) 481
BstMWI GCNNNNNNNGC 3 cut(s) 450, 695, 1013
BstNI CCWGG 3 cut(s) 516, 738, 762
BstNSI RCATGY 3 cut(s) 532, 1360, 1401
BstSCI CCNGG 4 cut(s) 514, 736, 760, 1240
BstSFI CTRYAG 2 cut(s) 187, 693
BstSLI GKGCMC 1 cut(s) 430
BstSNI TACGTA 1 cut(s) 550
BstUI CGCG 1 cut(s) 483
BstV1I GCAGC 4 cut(s) 522, 710, 1378, 1381
BstX2I RGATCY 1 cut(s) 562
BstYI RGATCY 1 cut(s) 562
BsuRI GGCC 3 cut(s) 52, 660, 1027
BtgI CCRYGG 1 cut(s) 505
BtsCI GGATG 2 cut(s) 95, 525
BtsIMutI CAGTG 2 cut(s) 432, 1303
Cac8I GCNNGC 2 cut(s) 530, 634
CaiI CAGNNNCTG 1 cut(s) 226
Cfr13I GGNCC 3 cut(s) 470, 658, 865
Csp6I GTAC 3 cut(s) 963, 1000, 1072
CviQI GTAC 3 cut(s) 963, 1000, 1072
DdeI CTNAG 2 cut(s) 86, 892
DpnI GATC 3 cut(s) 38, 564, 1164
DpnII GATC 3 cut(s) 36, 562, 1162
DriI GACNNNNNGTC 1 cut(s) 311
Eam1105I GACNNNNNGTC 1 cut(s) 311
Eco105I TACGTA 1 cut(s) 550
Eco130I CCWWGG 3 cut(s) 360, 505, 1028
Eco147I AGGCCT 1 cut(s) 52
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 2 cut(s) 470, 865
Eco57I CTGAAG 3 cut(s) 248, 599, 979
EcoNI CCTNNNNNAGG 2 cut(s) 653, 759
EcoO109I RGGNCCY 1 cut(s) 658
EcoRII CCWGG 3 cut(s) 514, 736, 760
EcoRV GATATC 1 cut(s) 130
EcoT14I CCWWGG 3 cut(s) 360, 505, 1028
EcoT22I ATGCAT 1 cut(s) 217
ErhI CCWWGG 3 cut(s) 360, 505, 1028
FalI AAGNNNNNCTT 2 cut(s) 812, 844
FaqI GGGAC 1 cut(s) 1280
FauNDI CATATG 1 cut(s) 1416
Fnu4HI GCNGC 5 cut(s) 445, 511, 699, 1392, 1395
FokI GGATG 2 cut(s) 82, 532
Fsp4HI GCNGC 5 cut(s) 445, 511, 699, 1392, 1395
FspBI CTAG 3 cut(s) 303, 750, 1253
GluI GCNGC 5 cut(s) 445, 511, 699, 1392, 1395
GsaI CCCAGC 1 cut(s) 168
GsuI CTGGAG 3 cut(s) 744, 759, 1389
HaeIII GGCC 3 cut(s) 52, 660, 1027
HapII CCGG 2 cut(s) 1241, 1379
HindIII AAGCTT 3 cut(s) 630, 1109, 1187
HinfI GANTC 5 cut(s) 240, 638, 726, 896, 1237
HpaII CCGG 2 cut(s) 1241, 1379
HphI GGTGA 3 cut(s) 96, 554, 1002
Hpy166II GTNNAC 2 cut(s) 239, 1002
Hpy188I TCNGA 5 cut(s) 159, 869, 1102, 1149, 1216
Hpy188III TCNNGA 2 cut(s) 171, 971
Hpy8I GTNNAC 2 cut(s) 239, 1002
HpyAV CCTTC 5 cut(s) 533, 674, 864, 1003, 1323
HpyCH4III ACNGT 1 cut(s) 1307
HpyCH4IV ACGT 2 cut(s) 549, 771
HpyCH4V TGCA 6 cut(s) 215, 284, 596, 913, 1362, 1397
HpyF10VI GCNNNNNNNGC 3 cut(s) 450, 695, 1013
HpyF3I CTNAG 2 cut(s) 86, 892
HpySE526I ACGT 2 cut(s) 549, 771
Kzo9I GATC 3 cut(s) 36, 562, 1162
LmnI GCTCC 2 cut(s) 645, 1103
Lsp1109I GCAGC 4 cut(s) 522, 710, 1378, 1381
LweI GCATC 4 cut(s) 202, 583, 1016, 1269
MaeI CTAG 3 cut(s) 303, 750, 1253
MaeII ACGT 2 cut(s) 549, 771
MaeIII GTNAC 4 cut(s) 28, 602, 815, 1372
MalI GATC 3 cut(s) 38, 564, 1164
MboI GATC 3 cut(s) 36, 562, 1162
MboII GAAGA 8 cut(s) 137, 241, 401, 592, 595, 940, 1058, 1237
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 1 cut(s) 562
MhlI GDGCHC 1 cut(s) 430
MluCI AATT 6 cut(s) 23, 534, 703, 914, 1140, 1311
MlyI GAGTC 3 cut(s) 234, 647, 1231
Mph1103I ATGCAT 1 cut(s) 217
MseI TTAA 3 cut(s) 384, 1275, 1435
MslI CAYNNNNRTG 2 cut(s) 572, 956
MspI CCGG 2 cut(s) 1241, 1379
MspR9I CCNGG 4 cut(s) 516, 738, 762, 1242
MunI CAATTG 1 cut(s) 534
Mva1269I GAATGC 1 cut(s) 284
MvaI CCWGG 3 cut(s) 516, 738, 762
MvnI CGCG 1 cut(s) 483
MwoI GCNNNNNNNGC 3 cut(s) 450, 695, 1013
NciI CCSGG 1 cut(s) 1242
NcoI CCATGG 1 cut(s) 505
NdeI CATATG 1 cut(s) 1416
NdeII GATC 3 cut(s) 36, 562, 1162
NlaIV GGNNCC 3 cut(s) 647, 1099, 1248
NmuCI GTSAC 2 cut(s) 28, 815
NsiI ATGCAT 1 cut(s) 217
NspI RCATGY 3 cut(s) 532, 1360, 1401
PaeI GCATGC 1 cut(s) 532
PasI CCCWGGG 1 cut(s) 515
PceI AGGCCT 1 cut(s) 52
PciI ACATGT 1 cut(s) 1356
PctI GAATGC 1 cut(s) 284
PfeI GAWTC 2 cut(s) 726, 896
PfoI TCCNGGA 2 cut(s) 736, 760
PkrI GCNGC 5 cut(s) 446, 512, 700, 1393, 1396
PleI GAGTC 3 cut(s) 234, 646, 1231
PpsI GAGTC 3 cut(s) 234, 646, 1231
Ppu21I YACGTR 1 cut(s) 550
PscI ACATGT 1 cut(s) 1356
PshBI ATTAAT 1 cut(s) 384
Psp6I CCWGG 3 cut(s) 514, 736, 760
PspFI CCCAGC 1 cut(s) 164
PspGI CCWGG 3 cut(s) 514, 736, 760
PspN4I GGNNCC 3 cut(s) 647, 1099, 1248
PspPI GGNCC 3 cut(s) 470, 658, 865
PstNI CAGNNNCTG 1 cut(s) 226
PsuI RGATCY 1 cut(s) 562
RsaI GTAC 3 cut(s) 964, 1001, 1073
RsaNI GTAC 3 cut(s) 963, 1000, 1072
RseI CAYNNNNRTG 2 cut(s) 572, 956
SaqAI TTAA 3 cut(s) 384, 1275, 1435
SatI GCNGC 5 cut(s) 445, 511, 699, 1392, 1395
Sau3AI GATC 3 cut(s) 36, 562, 1162
Sau96I GGNCC 3 cut(s) 470, 658, 865
SchI GAGTC 3 cut(s) 234, 647, 1231
ScrFI CCNGG 4 cut(s) 516, 738, 762, 1242
SduI GDGCHC 1 cut(s) 430
SfaNI GCATC 4 cut(s) 202, 583, 1016, 1269
SfcI CTRYAG 2 cut(s) 187, 693
SinI GGWCC 2 cut(s) 470, 865
SmiMI CAYNNNNRTG 2 cut(s) 572, 956
SmlI CTYRAG 3 cut(s) 806, 829, 1021
SmoI CTYRAG 3 cut(s) 806, 829, 1021
SnaBI TACGTA 1 cut(s) 550
SphI GCATGC 1 cut(s) 532
Sse9I AATT 6 cut(s) 23, 534, 703, 914, 1140, 1311
SseBI AGGCCT 1 cut(s) 52
SsiI CCGC 3 cut(s) 444, 468, 481
SspMI CTAG 3 cut(s) 303, 750, 1253
StuI AGGCCT 1 cut(s) 52
StyD4I CCNGG 4 cut(s) 514, 736, 760, 1240
StyI CCWWGG 3 cut(s) 360, 505, 1028
TaaI ACNGT 1 cut(s) 1307
TaiI ACGT 2 cut(s) 552, 774
TaqI TCGA 1 cut(s) 1080
TasI AATT 6 cut(s) 23, 534, 703, 914, 1140, 1311
TauI GCSGC 1 cut(s) 447
TfiI GAWTC 2 cut(s) 726, 896
Tru1I TTAA 3 cut(s) 384, 1275, 1435
Tru9I TTAA 3 cut(s) 384, 1275, 1435
TscAI CASTG 2 cut(s) 439, 1310
TseFI GTSAC 2 cut(s) 28, 815
TseI GCWGC 4 cut(s) 510, 698, 1391, 1394
Tsp45I GTSAC 2 cut(s) 28, 815
TspDTI ATGAA 7 cut(s) 256, 478, 873, 934, 1152, 1238, 1433
TspGWI ACGGA 1 cut(s) 153
TspRI CASTG 2 cut(s) 439, 1310
VpaK11BI GGWCC 2 cut(s) 470, 865
VspI ATTAAT 1 cut(s) 384
XagI CCTNNNNNAGG 2 cut(s) 653, 759
XapI RAATTY 3 cut(s) 703, 1140, 1311
XceI RCATGY 3 cut(s) 532, 1360, 1401
XspI CTAG 3 cut(s) 303, 750, 1253
Zsp2I ATGCAT 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.