Prupe.3G242200_v2.0.a1

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001085
Physical Location & Seq
Forward (+)
23522632 .. 23526795
4164 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G242200.1

Sequence Viewer

Length: 1206 bp
ATGGAAGAGCATGCACTCACTGAGAGCTTGAGTGAAATCTTTTTGAGCAATTTCTTTGAAAATCTCTTGGTAAGAGGTGCTGATGGTGAAAATGGCTCTCCAAGTACCACTAAGGATAAGGGGCAAAGATGGGCAACTCTAATGGCTGGGTCAAATGGTTACTACAACTATAGGCATCAGGCTGATATATGCCATGCATATCAGATACTGAAGAAAGATGGACTGAAAGATGAGAGAACATCATCATTGTTTTCATGTACGATGATACTGCACACAACCCGAAAAACCCTAGACAAGGTGTCATCGTCAACAAGCCAAAGGGCCATGACTGGAGGTAGTGGCAAGGTTCTGAAAAGTGGTCCAAATGACCATGTTTTCATCTATTATGCAGATAATGGTTCTGCAGGATTACTTGGGATGCCTAGTGAGGATGATTCTGTTTCTGCTAAAGATCTTATACATGTGCTAAGGAAGAAGCATCTTTCTAAATGTTTCAAAAGCATGGCTTTTATATGCATCGAAGCTTGCGAGTTTGCGAGCATATTTGAGGGCCTCCATCCAAATAAAATAAATATTTATGCTACTACCGCAGCAAATGCAGAAGAGAAATTTGGTTCCCACAGTAGTTATGGAACATATTGTTCAGGTGAACCTGAAGTTCCTGAGGAGTATGATACCTGTTTAGGAGACTTGTATAGTATTTCCTGGTTGGAGGATTCTTTCCAATATATTTTGAATCGTTGGCTCGTTCGAAGAAGAACAAATGAGTCTCATGTTATGCAGTATGGGGATGTGAGCCATAGAAAGGAGTTCCTCTTCACTTACATGGGTGCAGATCTTGTGAAGGATAGCTATAGTTCCATCGGTGATATCTCTTCACCCTCAGTCTCAAGGGCTGCTAACCAACGTGACACAAACCACCTTTATTTCCAGCACAAGAGCATCCATAAAATCGGGGAGCTTTTGTTTGGACACCAGAAGAGCTCAAACCTGTTGATGAACGTTAGGTCTCAGGGGCAACCTGTAGTAGATGATTGGGACTGCTTCAAGAAGTTTCTGAGGATTTATAAGAAGTACTGTGGACATTTATCAACATGCGAAATGAAATACACGCAAGCTATAGCCAACATATGCAATGCTGGGGTAACCATGGAAAAAATGGTTGCAGCTTCTGATCAAGCTTGTTCCAAGAAATCCCATGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

402

Amino Acids

45.02

Weight (kDa)

6.98

Isoelectric Point (pI)

44.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1068
AciI CCGC 1 cut(s) 588
AclI AACGTT 1 cut(s) 1002
AcsI RAATTY 1 cut(s) 608
AcuI CTGAAG 2 cut(s) 230, 675
AfaI GTAC 3 cut(s) 106, 259, 1076
AfiI CCNNNNNNNGG 2 cut(s) 295, 805
AflIII ACRYGT 1 cut(s) 460
AgsI TTSAA 4 cut(s) 59, 496, 736, 1048
AhdI GACNNNNNGTC 1 cut(s) 298
AjnI CCWGG 1 cut(s) 704
AjuI GAANNNNNNNTTGG 2 cut(s) 594, 626
AluBI AGCT 8 cut(s) 27, 524, 852, 961, 984, 1118, 1169, 1181
AluI AGCT 8 cut(s) 27, 524, 852, 961, 984, 1118, 1169, 1181
Alw21I GWGCWC 1 cut(s) 986
Alw26I GTCTC 4 cut(s) 681, 774, 892, 1014
AlwNI CAGNNNCTG 2 cut(s) 208, 1172
AoxI GGCC 2 cut(s) 321, 550
ApeKI GCWGC 3 cut(s) 590, 896, 1166
ApoI RAATTY 1 cut(s) 608
AspS9I GGNCC 3 cut(s) 321, 359, 550
AsuHPI GGTGA 4 cut(s) 98, 659, 870, 878
AsuII TTCGAA 1 cut(s) 751
AvaII GGWCC 1 cut(s) 359
AxyI CCTNAGG 1 cut(s) 663
BanII GRGCYC 1 cut(s) 986
Bbv12I GWGCWC 1 cut(s) 986
BbvI GCAGC 3 cut(s) 602, 883, 1178
BccI CCATC 5 cut(s) 77, 123, 212, 564, 869
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciT130I CCWGG 1 cut(s) 706
BclI TGATCA 1 cut(s) 1174
BcoDI GTCTC 4 cut(s) 681, 774, 892, 1014
BfaI CTAG 3 cut(s) 290, 423, 1204
BfmI CTRYAG 5 cut(s) 169, 402, 853, 1023, 1119
BglII AGATCT 2 cut(s) 451, 835
BisI GCNGC 3 cut(s) 591, 897, 1167
BlsI GCNGC 3 cut(s) 592, 898, 1168
BmcAI AGTACT 1 cut(s) 1076
Bme1390I CCNGG 1 cut(s) 706
Bme18I GGWCC 1 cut(s) 359
BmeRI GACNNNNNGTC 1 cut(s) 298
BmgT120I GGNCC 3 cut(s) 321, 359, 550
BmiI GGNNCC 1 cut(s) 616
BmrFI CCNGG 1 cut(s) 706
BmsI GCATC 5 cut(s) 184, 408, 487, 525, 951
BpmI CTGGAG 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 467
Bpu14I TTCGAA 1 cut(s) 751
BpuEI CTTGAG 2 cut(s) 49, 874
BsaI GGTCTC 1 cut(s) 1014
BsaJI CCNNGG 1 cut(s) 1149
BsaXI ACNNNNNCTCC 2 cut(s) 950, 980
Bsc4I CCNNNNNNNGG 2 cut(s) 295, 805
Bse1I ACTGG 1 cut(s) 334
Bse21I CCTNAGG 1 cut(s) 663
Bse3DI GCAATG 1 cut(s) 1141
BseBI CCWGG 1 cut(s) 706
BseDI CCNNGG 1 cut(s) 1149
BseGI GGATG 5 cut(s) 423, 436, 556, 796, 942
BseLI CCNNNNNNNGG 2 cut(s) 295, 805
BseMI GCAATG 1 cut(s) 1141
BseMII CTCAG 5 cut(s) 12, 654, 897, 1025, 1049
BseNI ACTGG 1 cut(s) 334
BseRI GAGGAG 1 cut(s) 680
BseXI GCAGC 3 cut(s) 602, 883, 1178
BseYI CCCAGC 2 cut(s) 146, 1139
BsgI GTGCAG 2 cut(s) 254, 852
BshFI GGCC 2 cut(s) 323, 552
BsiHKAI GWGCWC 1 cut(s) 986
BslFI GGGAC 1 cut(s) 1052
BslI CCNNNNNNNGG 2 cut(s) 295, 805
BsmAI GTCTC 4 cut(s) 681, 774, 892, 1014
BsmFI GGGAC 1 cut(s) 1052
BsnI GGCC 2 cut(s) 323, 552
Bso31I GGTCTC 1 cut(s) 1014
Bsp119I TTCGAA 1 cut(s) 751
Bsp1286I GDGCHC 1 cut(s) 986
Bsp143I GATC 3 cut(s) 451, 835, 1174
Bsp19I CCATGG 1 cut(s) 1149
BspACI CCGC 1 cut(s) 588
BspANI GGCC 2 cut(s) 323, 552
BspCNI CTCAG 5 cut(s) 13, 655, 896, 1024, 1050
BspLI GGNNCC 1 cut(s) 616
BspMAI CTGCAG 1 cut(s) 406
BspQI GCTCTTC 1 cut(s) 974
BspT104I TTCGAA 1 cut(s) 751
BspTNI GGTCTC 1 cut(s) 1014
BsrDI GCAATG 1 cut(s) 1141
BsrI ACTGG 1 cut(s) 334
BssECI CCNNGG 1 cut(s) 1149
BssMI GATC 3 cut(s) 451, 835, 1174
BssT1I CCWWGG 1 cut(s) 1149
Bst2UI CCWGG 1 cut(s) 706
Bst4CI ACNGT 2 cut(s) 623, 1079
Bst6I CTCTTC 4 cut(s) 597, 821, 880, 974
BstAPI GCANNNNNTGC 1 cut(s) 596
BstBI TTCGAA 1 cut(s) 751
BstC8I GCNNGC 4 cut(s) 12, 526, 538, 1116
BstDEI CTNAG 7 cut(s) 21, 111, 467, 663, 883, 1011, 1058
BstDSI CCRYGG 1 cut(s) 1149
BstEII GGTNACC 1 cut(s) 1144
BstENI CCTNNNNNAGG 1 cut(s) 293
BstF5I GGATG 5 cut(s) 423, 436, 556, 796, 942
BstKTI GATC 3 cut(s) 454, 838, 1177
BstMAI GTCTC 4 cut(s) 681, 774, 892, 1014
BstMBI GATC 3 cut(s) 451, 835, 1174
BstMWI GCNNNNNNNGC 2 cut(s) 587, 596
BstNI CCWGG 1 cut(s) 706
BstNSI RCATGY 3 cut(s) 14, 464, 1098
BstPI GGTNACC 1 cut(s) 1144
BstSCI CCNGG 1 cut(s) 704
BstSFI CTRYAG 5 cut(s) 169, 402, 853, 1023, 1119
BstV1I GCAGC 3 cut(s) 602, 883, 1178
BstX2I RGATCY 2 cut(s) 451, 835
BstYI RGATCY 2 cut(s) 451, 835
Bsu36I CCTNAGG 1 cut(s) 663
BsuRI GGCC 2 cut(s) 323, 552
BtgI CCRYGG 1 cut(s) 1149
BtsCI GGATG 5 cut(s) 423, 436, 556, 796, 942
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 4 cut(s) 12, 526, 538, 1116
CaiI CAGNNNCTG 2 cut(s) 208, 1172
Cfr13I GGNCC 3 cut(s) 321, 359, 550
Csp6I GTAC 3 cut(s) 105, 258, 1075
CviQI GTAC 3 cut(s) 105, 258, 1075
DdeI CTNAG 7 cut(s) 21, 111, 467, 663, 883, 1011, 1058
DpnI GATC 3 cut(s) 453, 837, 1176
DpnII GATC 3 cut(s) 451, 835, 1174
DriI GACNNNNNGTC 1 cut(s) 298
Eam1104I CTCTTC 4 cut(s) 597, 821, 880, 974
Eam1105I GACNNNNNGTC 1 cut(s) 298
EarI CTCTTC 4 cut(s) 597, 821, 880, 974
Ecl136II GAGCTC 1 cut(s) 984
Eco130I CCWWGG 1 cut(s) 1149
Eco24I GRGCYC 1 cut(s) 986
Eco31I GGTCTC 1 cut(s) 1014
Eco32I GATATC 1 cut(s) 871
Eco47I GGWCC 1 cut(s) 359
Eco53kI GAGCTC 1 cut(s) 984
Eco57I CTGAAG 2 cut(s) 230, 675
Eco81I CCTNAGG 1 cut(s) 663
Eco91I GGTNACC 1 cut(s) 1144
EcoICRI GAGCTC 1 cut(s) 984
EcoNI CCTNNNNNAGG 1 cut(s) 293
EcoO109I RGGNCCY 1 cut(s) 550
EcoO65I GGTNACC 1 cut(s) 1144
EcoRII CCWGG 1 cut(s) 704
EcoRV GATATC 1 cut(s) 871
EcoT14I CCWWGG 1 cut(s) 1149
EcoT22I ATGCAT 2 cut(s) 199, 518
EcoT38I GRGCYC 1 cut(s) 986
ErhI CCWWGG 1 cut(s) 1149
FalI AAGNNNNNCTT 2 cut(s) 490, 522
FaqI GGGAC 1 cut(s) 1052
FauNDI CATATG 1 cut(s) 1130
FbaI TGATCA 1 cut(s) 1174
Fnu4HI GCNGC 3 cut(s) 591, 897, 1167
FokI GGATG 5 cut(s) 430, 443, 543, 803, 929
FriOI GRGCYC 1 cut(s) 986
Fsp4HI GCNGC 3 cut(s) 591, 897, 1167
FspBI CTAG 3 cut(s) 290, 423, 1204
GluI GCNGC 3 cut(s) 591, 897, 1167
GsaI CCCAGC 2 cut(s) 150, 1143
GsuI CTGGAG 1 cut(s) 351
HaeIII GGCC 2 cut(s) 323, 552
HincII GTYRAC 1 cut(s) 309
HindII GTYRAC 1 cut(s) 309
HindIII AAGCTT 2 cut(s) 522, 1179
HinfI GANTC 4 cut(s) 434, 716, 736, 767
HphI GGTGA 4 cut(s) 98, 659, 870, 878
Hpy166II GTNNAC 3 cut(s) 309, 650, 1082
Hpy188I TCNGA 4 cut(s) 204, 351, 1059, 1174
Hpy188III TCNNGA 2 cut(s) 662, 1048
Hpy8I GTNNAC 3 cut(s) 309, 650, 1082
HpyAV CCTTC 1 cut(s) 838
HpyCH4III ACNGT 2 cut(s) 623, 1079
HpyCH4IV ACGT 2 cut(s) 907, 1002
HpyF10VI GCNNNNNNNGC 2 cut(s) 587, 596
HpyF3I CTNAG 7 cut(s) 21, 111, 467, 663, 883, 1011, 1058
HpySE526I ACGT 2 cut(s) 907, 1002
Ksp22I TGATCA 1 cut(s) 1174
Kzo9I GATC 3 cut(s) 451, 835, 1174
LguI GCTCTTC 1 cut(s) 974
LmnI GCTCC 1 cut(s) 958
Lsp1109I GCAGC 3 cut(s) 602, 883, 1178
LweI GCATC 5 cut(s) 184, 408, 487, 525, 951
MaeI CTAG 3 cut(s) 290, 423, 1204
MaeII ACGT 2 cut(s) 907, 1002
MaeIII GTNAC 3 cut(s) 158, 908, 1144
MalI GATC 3 cut(s) 453, 837, 1176
MboI GATC 3 cut(s) 451, 835, 1174
MboII GAAGA 9 cut(s) 17, 223, 484, 614, 765, 768, 808, 867, 991
MflI RGATCY 2 cut(s) 451, 835
MhlI GDGCHC 1 cut(s) 986
MluCI AATT 2 cut(s) 49, 608
MlyI GAGTC 1 cut(s) 776
MmeI TCCRAC 1 cut(s) 690
Mph1103I ATGCAT 2 cut(s) 199, 518
MslI CAYNNNNRTG 1 cut(s) 824
MspR9I CCNGG 1 cut(s) 706
MvaI CCWGG 1 cut(s) 706
MwoI GCNNNNNNNGC 2 cut(s) 587, 596
NcoI CCATGG 1 cut(s) 1149
NdeI CATATG 1 cut(s) 1130
NdeII GATC 3 cut(s) 451, 835, 1174
NlaIV GGNNCC 1 cut(s) 616
NmuCI GTSAC 1 cut(s) 908
NsiI ATGCAT 2 cut(s) 199, 518
NspI RCATGY 3 cut(s) 14, 464, 1098
NspV TTCGAA 1 cut(s) 751
PaeI GCATGC 1 cut(s) 14
PciI ACATGT 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 974
PcsI WCGNNNNNNNCGW 1 cut(s) 525
PfeI GAWTC 3 cut(s) 434, 716, 736
PkrI GCNGC 3 cut(s) 592, 898, 1168
PleI GAGTC 1 cut(s) 775
PpsI GAGTC 1 cut(s) 775
PscI ACATGT 1 cut(s) 460
PsiI TTATAA 1 cut(s) 1068
Psp124BI GAGCTC 1 cut(s) 986
Psp1406I AACGTT 1 cut(s) 1002
Psp6I CCWGG 1 cut(s) 704
PspEI GGTNACC 1 cut(s) 1144
PspFI CCCAGC 2 cut(s) 146, 1139
PspGI CCWGG 1 cut(s) 704
PspN4I GGNNCC 1 cut(s) 616
PspPI GGNCC 3 cut(s) 321, 359, 550
PstI CTGCAG 1 cut(s) 406
PstNI CAGNNNCTG 2 cut(s) 208, 1172
PsuI RGATCY 2 cut(s) 451, 835
RsaI GTAC 3 cut(s) 106, 259, 1076
RsaNI GTAC 3 cut(s) 105, 258, 1075
RseI CAYNNNNRTG 1 cut(s) 824
SacI GAGCTC 1 cut(s) 986
SapI GCTCTTC 1 cut(s) 974
SatI GCNGC 3 cut(s) 591, 897, 1167
Sau3AI GATC 3 cut(s) 451, 835, 1174
Sau96I GGNCC 3 cut(s) 321, 359, 550
ScaI AGTACT 1 cut(s) 1076
SchI GAGTC 1 cut(s) 776
ScrFI CCNGG 1 cut(s) 706
SduI GDGCHC 1 cut(s) 986
SfaNI GCATC 5 cut(s) 184, 408, 487, 525, 951
SfcI CTRYAG 5 cut(s) 169, 402, 853, 1023, 1119
SfuI TTCGAA 1 cut(s) 751
SinI GGWCC 1 cut(s) 359
SmiMI CAYNNNNRTG 1 cut(s) 824
SmlI CTYRAG 2 cut(s) 28, 889
SmoI CTYRAG 2 cut(s) 28, 889
SphI GCATGC 1 cut(s) 14
Sse9I AATT 2 cut(s) 49, 608
SsiI CCGC 1 cut(s) 588
SspI AATATT 1 cut(s) 574
SspMI CTAG 3 cut(s) 290, 423, 1204
SstI GAGCTC 1 cut(s) 986
StyD4I CCNGG 1 cut(s) 704
StyI CCWWGG 1 cut(s) 1149
TaaI ACNGT 2 cut(s) 623, 1079
TaiI ACGT 2 cut(s) 910, 1005
TaqI TCGA 2 cut(s) 519, 751
TasI AATT 2 cut(s) 49, 608
TatI WGTACW 1 cut(s) 1074
TfiI GAWTC 3 cut(s) 434, 716, 736
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 1 cut(s) 908
TseI GCWGC 3 cut(s) 590, 896, 1166
Tsp45I GTSAC 1 cut(s) 908
TspDTI ATGAA 4 cut(s) 243, 367, 1013, 1118
TspRI CASTG 1 cut(s) 25
VpaK11BI GGWCC 1 cut(s) 359
XagI CCTNNNNNAGG 1 cut(s) 293
XapI RAATTY 1 cut(s) 608
XceI RCATGY 3 cut(s) 14, 464, 1098
XcmI CCANNNNNNNNNTGG 2 cut(s) 626, 1156
XspI CTAG 3 cut(s) 290, 423, 1204
ZrmI AGTACT 1 cut(s) 1076
Zsp2I ATGCAT 2 cut(s) 199, 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.