FvH4_5g23280

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001085
Physical Location & Seq
Forward (+)
14612836 .. 14615554
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23280.t1

Sequence Viewer

Length: 1392 bp
ATGAGAGGACAGTACTGTTCAAGCGGCGCATTTTTGATCTTGCTTGCCATCATTGGTGTGTGCAATGCTCAACTTTCCAACTTGATGCCACAAGAATTTCCAGAGGAACTCATCATTCATGTTGATAGGGGTTCTAGCAACAGTGACACAAATGGAACAAGATGGGCAGTTCTTGTTGCTGGCTCTATGGGTTATGGCAACTATAGGCATCAGGCTGATGTTTGTCATGCATATCAAATTTTGAAGAATGGTGGACTCAAAGATGAAAACATCATTGTTTTCATGTACGATGACATTGCAAACAACCCTGAGAATCCAAGGCCTGGTGTCATCATCAACAAGCCCGACGGCGGCGATGTTTACCATGGAGTTCCCAAGGATTACGCAGGAGAGAATGCTACTGCTGCTAATCTCTATGCTGTCATTCTCGGAGATAAATCAAATCTCTCTGGGGGTAGTGGCAAAGTTCTGAACAGCGGGCCAAATGACAATGTCTTCATCTATTACACCGACCATGGAGGGCCTGGGATCATCGGGATGCCTGAAGGCGATTTTGTGTATGCCGATGATCTGGTGGGTGTTCTAATAAAGAAGCATGAAGCTAATGCATACAAAAACATGGTATTTTATCTCGAAGCTTGTGAATCCGGGAGTATGTTCGAGGGTCTACTCCCAACAAATATAAGCATTTATGCAACCACAGCTTCAAATGCAACTGAGAGTAGTTGGGGAACTTACTGCCCTGATATGGATCCTCCTCCACCTCCTGAATATGACACTTGCTTGGGTGATTTGTACAGTGTTTCTTGGATGGAGGACTCTGACTTGCAAGATTTACGCCAAGAGACTCTGGAAGAGCAATATCAATCGGTTAGATGGAGGACAGTAAATAATACGTATGGTTCTCATGTTACTCAATATGGAAATATGTATCAATCCTCAGATGCAGTATCCTTTTACTTGGGTGCAAATGATAACTCTACCTCCATCGAGAGCAAATCGACACAATCAATCTTAGAAGTTGTTGACAATCGCGATGCAGATCTTCTTCATCTGTGGCACAAGTTCCAACGTGCACCCGCTGGTTCTGAATTGAAGCAGGAAGCTAAAAGGGTTCTGGATGAAGAAATCTCAAGCAGGCAGCGCATCGACAACAACATAAACCAAATCGGACAACTTGTGTTGGGAGATTCATCAGGTAACTCTGTTCGTCCCGGGAGGCAAGCTCTTGTTGATGATTGGGACTGCTTTAAGGCGATTGTGAAAACTTATGAGAAACATTGTGGAAAATTGTCAAATTATGGAATGAAGCACACACGAGTGTTTGCCAATATGTGCAATGCTGGGATCAGCGTGGACCAAGTGGTTGCTGCATCAGCTCGAACTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

464

Amino Acids

50.88

Weight (kDa)

4.8

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 54 - 322 4.4e-114 Peptidase C13 family
Legum_prodom PF20985 373 - 463 4.5e-33 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 323
AccI GTMKAC 1 cut(s) 667
AccII CGCG 1 cut(s) 1035
AciI CCGC 4 cut(s) 24, 351, 477, 1080
AclWI GGATC 4 cut(s) 536, 746, 759, 1355
AcsI RAATTY 2 cut(s) 95, 237
AcuI CTGAAG 1 cut(s) 564
AfaI GTAC 3 cut(s) 14, 287, 797
AfiI CCNNNNNNNGG 3 cut(s) 323, 350, 748
AgsI TTSAA 4 cut(s) 21, 244, 708, 1096
AjnI CCWGG 2 cut(s) 322, 523
AleI CACNNNNGTG 1 cut(s) 1319
AluBI AGCT 6 cut(s) 602, 638, 704, 1106, 1226, 1379
AluI AGCT 6 cut(s) 602, 638, 704, 1106, 1226, 1379
Alw21I GWGCWC 1 cut(s) 1078
Alw26I GTCTC 1 cut(s) 839
Alw44I GTGCAC 1 cut(s) 1074
AlwI GGATC 4 cut(s) 536, 746, 759, 1355
Ama87I CYCGRG 1 cut(s) 1214
AoxI GGCC 3 cut(s) 320, 479, 521
ApaLI GTGCAC 1 cut(s) 1074
ApeKI GCWGC 3 cut(s) 404, 1141, 1370
ApoI RAATTY 2 cut(s) 95, 237
AspLEI GCGC 2 cut(s) 29, 1146
AspS9I GGNCC 3 cut(s) 479, 521, 1357
AsuC2I CCSGG 3 cut(s) 649, 1215, 1216
AsuHPI GGTGA 1 cut(s) 800
AvaI CYCGRG 1 cut(s) 1214
AvaII GGWCC 1 cut(s) 1357
BaeGI GKGCMC 1 cut(s) 1078
BamHI GGATCC 1 cut(s) 751
BauI CACGAG 1 cut(s) 1317
BbsI GAAGAC 1 cut(s) 487
Bbv12I GWGCWC 1 cut(s) 1078
BbvI GCAGC 3 cut(s) 391, 1153, 1357
BccI CCATC 5 cut(s) 56, 156, 805, 870, 995
BceAI ACGGC 1 cut(s) 364
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 2 cut(s) 324, 525
BciVI GTATCC 1 cut(s) 961
BcnI CCSGG 3 cut(s) 649, 1215, 1216
BcoDI GTCTC 1 cut(s) 839
BfaI CTAG 2 cut(s) 135, 1390
BfmI CTRYAG 1 cut(s) 202
BfuI GTATCC 1 cut(s) 961
BglII AGATCT 1 cut(s) 1042
BisI GCNGC 5 cut(s) 25, 352, 405, 1142, 1371
BlsI GCNGC 5 cut(s) 26, 353, 406, 1143, 1372
BmcAI AGTACT 1 cut(s) 14
Bme1390I CCNGG 5 cut(s) 324, 525, 649, 1215, 1216
Bme18I GGWCC 1 cut(s) 1357
BmeT110I CYCGRG 1 cut(s) 1214
BmgT120I GGNCC 3 cut(s) 479, 521, 1357
BmiI GGNNCC 1 cut(s) 753
BmrFI CCNGG 5 cut(s) 324, 525, 649, 1215, 1216
BmsI GCATC 7 cut(s) 75, 217, 528, 934, 1027, 1155, 1382
BpiI GAAGAC 1 cut(s) 487
BplI GAGNNNNNCTC 2 cut(s) 1210, 1242
BpuEI CTTGAG 1 cut(s) 1117
BpuMI CCSGG 3 cut(s) 649, 1215, 1216
BsaAI YACGTR 1 cut(s) 897
BsaBI GATNNNNATC 2 cut(s) 750, 1041
BsaJI CCNNGG 6 cut(s) 317, 364, 375, 514, 524, 1214
BsaXI ACNNNNNCTCC 2 cut(s) 968, 998
Bsc4I CCNNNNNNNGG 3 cut(s) 323, 350, 748
Bse3DI GCAATG 3 cut(s) 70, 294, 1345
Bse8I GATNNNNATC 2 cut(s) 750, 1041
BseBI CCWGG 2 cut(s) 324, 525
BseDI CCNNGG 6 cut(s) 317, 364, 375, 514, 524, 1214
BseGI GGATG 3 cut(s) 543, 816, 1126
BseJI GATNNNNATC 2 cut(s) 750, 1041
BseLI CCNNNNNNNGG 3 cut(s) 323, 350, 748
BseMI GCAATG 3 cut(s) 70, 294, 1345
BseMII CTCAG 3 cut(s) 300, 708, 954
BseRI GAGGAG 1 cut(s) 747
BseSI GKGCMC 1 cut(s) 1078
BseXI GCAGC 3 cut(s) 391, 1153, 1357
BseYI CCCAGC 1 cut(s) 1343
Bsh1236I CGCG 1 cut(s) 1035
BshFI GGCC 3 cut(s) 322, 481, 523
BsiHKAI GWGCWC 1 cut(s) 1078
BsiHKCI CYCGRG 1 cut(s) 1214
BsiSI CCGG 2 cut(s) 648, 1215
BslFI GGGAC 2 cut(s) 1197, 1256
BslI CCNNNNNNNGG 3 cut(s) 323, 350, 748
BsmAI GTCTC 1 cut(s) 839
BsmFI GGGAC 2 cut(s) 1197, 1256
BsmI GAATGC 1 cut(s) 400
BsnI GGCC 3 cut(s) 322, 481, 523
BsoBI CYCGRG 1 cut(s) 1214
Bsp1286I GDGCHC 1 cut(s) 1078
Bsp1407I TGTACA 1 cut(s) 795
Bsp143I GATC 6 cut(s) 36, 528, 568, 751, 1042, 1347
Bsp19I CCATGG 2 cut(s) 364, 514
Bsp68I TCGCGA 1 cut(s) 1035
BspACI CCGC 4 cut(s) 24, 351, 477, 1080
BspANI GGCC 3 cut(s) 322, 481, 523
BspCNI CTCAG 3 cut(s) 301, 709, 953
BspFNI CGCG 1 cut(s) 1035
BspLI GGNNCC 1 cut(s) 753
BspPI GGATC 4 cut(s) 536, 746, 759, 1355
BspQI GCTCTTC 1 cut(s) 849
BsrDI GCAATG 3 cut(s) 70, 294, 1345
BsrGI TGTACA 1 cut(s) 795
BssECI CCNNGG 6 cut(s) 317, 364, 375, 514, 524, 1214
BssMI GATC 6 cut(s) 36, 528, 568, 751, 1042, 1347
BssSI CACGAG 1 cut(s) 1317
BssT1I CCWWGG 4 cut(s) 317, 364, 375, 514
Bst2BI CACGAG 1 cut(s) 1317
Bst2UI CCWGG 2 cut(s) 324, 525
Bst4CI ACNGT 5 cut(s) 12, 17, 143, 800, 886
Bst6I CTCTTC 1 cut(s) 849
BstAUI TGTACA 1 cut(s) 795
BstBAI YACGTR 1 cut(s) 897
BstC8I GCNNGC 5 cut(s) 45, 181, 479, 1139, 1224
BstDEI CTNAG 4 cut(s) 309, 717, 940, 1015
BstDSI CCRYGG 2 cut(s) 364, 514
BstF5I GGATG 3 cut(s) 543, 816, 1126
BstFNI CGCG 1 cut(s) 1035
BstHHI GCGC 2 cut(s) 29, 1146
BstKTI GATC 6 cut(s) 39, 531, 571, 754, 1045, 1350
BstMAI GTCTC 1 cut(s) 839
BstMBI GATC 6 cut(s) 36, 528, 568, 751, 1042, 1347
BstMWI GCNNNNNNNGC 5 cut(s) 404, 701, 710, 1143, 1376
BstNI CCWGG 2 cut(s) 324, 525
BstSCI CCNGG 5 cut(s) 322, 523, 647, 1213, 1214
BstSFI CTRYAG 1 cut(s) 202
BstSLI GKGCMC 1 cut(s) 1078
BstSNI TACGTA 1 cut(s) 897
BstUI CGCG 1 cut(s) 1035
BstV1I GCAGC 3 cut(s) 391, 1153, 1357
BstV2I GAAGAC 1 cut(s) 487
BstX2I RGATCY 2 cut(s) 751, 1042
BstYI RGATCY 2 cut(s) 751, 1042
BsuI GTATCC 1 cut(s) 961
BsuRI GGCC 3 cut(s) 322, 481, 523
BtgI CCRYGG 2 cut(s) 364, 514
BtgZI GCGATG 2 cut(s) 369, 1050
BtsCI GGATG 3 cut(s) 543, 816, 1126
BtsIMutI CAGTG 2 cut(s) 148, 805
BtuMI TCGCGA 1 cut(s) 1035
Cac8I GCNNGC 5 cut(s) 45, 181, 479, 1139, 1224
CfoI GCGC 2 cut(s) 29, 1146
Cfr13I GGNCC 3 cut(s) 479, 521, 1357
Cfr9I CCCGGG 1 cut(s) 1214
Csp6I GTAC 3 cut(s) 13, 286, 796
CviAII CATG 8 cut(s) 119, 227, 283, 365, 515, 596, 619, 908
CviQI GTAC 3 cut(s) 13, 286, 796
DdeI CTNAG 4 cut(s) 309, 717, 940, 1015
DpnI GATC 6 cut(s) 38, 530, 570, 753, 1044, 1349
DpnII GATC 6 cut(s) 36, 528, 568, 751, 1042, 1347
Eam1104I CTCTTC 1 cut(s) 849
EarI CTCTTC 1 cut(s) 849
Eco105I TACGTA 1 cut(s) 897
Eco130I CCWWGG 4 cut(s) 317, 364, 375, 514
Eco147I AGGCCT 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 1357
Eco57I CTGAAG 1 cut(s) 564
Eco88I CYCGRG 1 cut(s) 1214
EcoO109I RGGNCCY 1 cut(s) 521
EcoRII CCWGG 2 cut(s) 322, 523
EcoT14I CCWWGG 4 cut(s) 317, 364, 375, 514
EcoT22I ATGCAT 2 cut(s) 232, 610
ErhI CCWWGG 4 cut(s) 317, 364, 375, 514
FaeI CATG 8 cut(s) 122, 230, 286, 368, 518, 599, 622, 911
FaqI GGGAC 2 cut(s) 1197, 1256
FatI CATG 8 cut(s) 118, 226, 282, 364, 514, 595, 618, 907
FauI CCCGC 2 cut(s) 470, 1087
FblI GTMKAC 1 cut(s) 667
Fnu4HI GCNGC 5 cut(s) 25, 352, 405, 1142, 1371
FokI GGATG 3 cut(s) 550, 823, 1133
Fsp4HI GCNGC 5 cut(s) 25, 352, 405, 1142, 1371
FspBI CTAG 2 cut(s) 135, 1390
GlaI GCGC 2 cut(s) 28, 1145
GluI GCNGC 5 cut(s) 25, 352, 405, 1142, 1371
GsaI CCCAGC 1 cut(s) 1347
HaeIII GGCC 3 cut(s) 322, 481, 523
HapII CCGG 2 cut(s) 648, 1215
HhaI GCGC 2 cut(s) 29, 1146
Hin1II CATG 8 cut(s) 122, 230, 286, 368, 518, 599, 622, 911
Hin6I GCGC 2 cut(s) 27, 1144
HinP1I GCGC 2 cut(s) 27, 1144
HincII GTYRAC 1 cut(s) 1027
HindII GTYRAC 1 cut(s) 1027
HindIII AAGCTT 1 cut(s) 636
HinfI GANTC 6 cut(s) 255, 313, 644, 818, 847, 1190
HpaII CCGG 2 cut(s) 648, 1215
HphI GGTGA 1 cut(s) 800
Hpy166II GTNNAC 6 cut(s) 254, 361, 668, 1027, 1076, 1357
Hpy188I TCNGA 6 cut(s) 431, 471, 823, 943, 1090, 1172
Hpy188III TCNNGA 8 cut(s) 101, 535, 632, 767, 851, 991, 1034, 1118
Hpy8I GTNNAC 6 cut(s) 254, 361, 668, 1027, 1076, 1357
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 1 cut(s) 539
HpyCH4III ACNGT 5 cut(s) 12, 17, 143, 800, 886
HpyCH4IV ACGT 2 cut(s) 896, 1072
HpyF10VI GCNNNNNNNGC 5 cut(s) 404, 701, 710, 1143, 1376
HpyF3I CTNAG 4 cut(s) 309, 717, 940, 1015
HpySE526I ACGT 2 cut(s) 896, 1072
Hsp92II CATG 8 cut(s) 122, 230, 286, 368, 518, 599, 622, 911
HspAI GCGC 2 cut(s) 27, 1144
Kzo9I GATC 6 cut(s) 36, 528, 568, 751, 1042, 1347
LguI GCTCTTC 1 cut(s) 849
Lsp1109I GCAGC 3 cut(s) 391, 1153, 1357
LweI GCATC 7 cut(s) 75, 217, 528, 934, 1027, 1155, 1382
MaeI CTAG 2 cut(s) 135, 1390
MaeII ACGT 2 cut(s) 896, 1072
MaeIII GTNAC 3 cut(s) 143, 910, 1199
MalI GATC 6 cut(s) 38, 530, 570, 753, 1044, 1349
MboI GATC 6 cut(s) 36, 528, 568, 751, 1042, 1347
MboII GAAGA 6 cut(s) 256, 487, 866, 1037, 1040, 1136
MflI RGATCY 2 cut(s) 751, 1042
MhlI GDGCHC 1 cut(s) 1078
MluCI AATT 5 cut(s) 95, 237, 1091, 1289, 1297
MlyI GAGTC 3 cut(s) 249, 812, 841
MmeI TCCRAC 2 cut(s) 102, 1093
Mph1103I ATGCAT 2 cut(s) 232, 610
MseI TTAA 1 cut(s) 1251
MslI CAYNNNNRTG 3 cut(s) 56, 536, 1319
MspA1I CMGCKG 2 cut(s) 477, 1082
MspI CCGG 2 cut(s) 648, 1215
MspR9I CCNGG 5 cut(s) 324, 525, 649, 1215, 1216
Mva1269I GAATGC 1 cut(s) 400
MvaI CCWGG 2 cut(s) 324, 525
MvnI CGCG 1 cut(s) 1035
MwoI GCNNNNNNNGC 5 cut(s) 404, 701, 710, 1143, 1376
NciI CCSGG 3 cut(s) 649, 1215, 1216
NcoI CCATGG 2 cut(s) 364, 514
NdeII GATC 6 cut(s) 36, 528, 568, 751, 1042, 1347
NlaIII CATG 8 cut(s) 122, 230, 286, 368, 518, 599, 622, 911
NlaIV GGNNCC 1 cut(s) 753
NmuCI GTSAC 1 cut(s) 143
NruI TCGCGA 1 cut(s) 1035
NsiI ATGCAT 2 cut(s) 232, 610
OliI CACNNNNGTG 1 cut(s) 1319
PceI AGGCCT 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 849
PctI GAATGC 1 cut(s) 400
PfeI GAWTC 3 cut(s) 313, 644, 1190
PflFI GACNNNGTC 1 cut(s) 491
PflMI CCANNNNNTGG 1 cut(s) 323
PfoI TCCNGGA 1 cut(s) 647
PkrI GCNGC 5 cut(s) 26, 353, 406, 1143, 1372
PleI GAGTC 3 cut(s) 249, 812, 841
PpsI GAGTC 3 cut(s) 249, 812, 841
Ppu21I YACGTR 1 cut(s) 897
Psp6I CCWGG 2 cut(s) 322, 523
PspFI CCCAGC 1 cut(s) 1343
PspGI CCWGG 2 cut(s) 322, 523
PspN4I GGNNCC 1 cut(s) 753
PspPI GGNCC 3 cut(s) 479, 521, 1357
PsuI RGATCY 2 cut(s) 751, 1042
PsyI GACNNNGTC 1 cut(s) 491
RruI TCGCGA 1 cut(s) 1035
RsaI GTAC 3 cut(s) 14, 287, 797
RsaNI GTAC 3 cut(s) 13, 286, 796
RseI CAYNNNNRTG 3 cut(s) 56, 536, 1319
SapI GCTCTTC 1 cut(s) 849
SaqAI TTAA 1 cut(s) 1251
SatI GCNGC 5 cut(s) 25, 352, 405, 1142, 1371
Sau3AI GATC 6 cut(s) 36, 528, 568, 751, 1042, 1347
Sau96I GGNCC 3 cut(s) 479, 521, 1357
ScaI AGTACT 1 cut(s) 14
SchI GAGTC 3 cut(s) 249, 812, 841
ScrFI CCNGG 5 cut(s) 324, 525, 649, 1215, 1216
SduI GDGCHC 1 cut(s) 1078
SfaNI GCATC 7 cut(s) 75, 217, 528, 934, 1027, 1155, 1382
SfcI CTRYAG 1 cut(s) 202
SinI GGWCC 1 cut(s) 1357
SmaI CCCGGG 1 cut(s) 1216
SmiMI CAYNNNNRTG 3 cut(s) 56, 536, 1319
SmlI CTYRAG 1 cut(s) 1132
SmoI CTYRAG 1 cut(s) 1132
SnaBI TACGTA 1 cut(s) 897
Sse9I AATT 5 cut(s) 95, 237, 1091, 1289, 1297
SseBI AGGCCT 1 cut(s) 322
SsiI CCGC 4 cut(s) 24, 351, 477, 1080
SspMI CTAG 2 cut(s) 135, 1390
StuI AGGCCT 1 cut(s) 322
StyD4I CCNGG 5 cut(s) 322, 523, 647, 1213, 1214
StyI CCWWGG 4 cut(s) 317, 364, 375, 514
TaaI ACNGT 5 cut(s) 12, 17, 143, 800, 886
TaiI ACGT 2 cut(s) 899, 1075
TaqI TCGA 6 cut(s) 633, 660, 990, 1001, 1149, 1381
TasI AATT 5 cut(s) 95, 237, 1091, 1289, 1297
TatI WGTACW 2 cut(s) 12, 795
TauI GCSGC 2 cut(s) 27, 354
TfiI GAWTC 3 cut(s) 313, 644, 1190
Tru1I TTAA 1 cut(s) 1251
Tru9I TTAA 1 cut(s) 1251
TscAI CASTG 2 cut(s) 148, 805
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 3 cut(s) 404, 1141, 1370
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 9 cut(s) 107, 271, 279, 487, 612, 1040, 1137, 1182, 1322
TspMI CCCGGG 1 cut(s) 1214
TspRI CASTG 2 cut(s) 148, 805
Tth111I GACNNNGTC 1 cut(s) 491
Van91I CCANNNNNTGG 1 cut(s) 323
VneI GTGCAC 1 cut(s) 1074
VpaK11BI GGWCC 1 cut(s) 1357
XapI RAATTY 2 cut(s) 95, 237
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XmaI CCCGGG 1 cut(s) 1214
XmiI GTMKAC 1 cut(s) 667
XspI CTAG 2 cut(s) 135, 1390
ZrmI AGTACT 1 cut(s) 14
Zsp2I ATGCAT 2 cut(s) 232, 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.