Rmu_sc0002345.1_g000004

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002345.1
OG0001085
Physical Location & Seq
Reverse (-)
10728 .. 11455
728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002345.1_g000004.1.cds

Sequence Viewer

Length: 474 bp
atgcagtatggagatatgagtcacataaaggaattcctctacacttacatgggggcaactcctaaaactcatagctatgctcccttcacatcctcgccttcggaatcaatctcaaggactttaaacagagctccaactggctctcatgagaagatcgaagctcaaaagcagctacgtaaagaaattgctcgaaggaaacatgtggactacagcataaccaaaataggggagcttctgtttgggaccgagaggagctcaaaagtattgatgaatgtcaggccttcgggacaacctctagtggatgactgggagtgcttcaagatgtttataaggacttatgagagtcgctgtggaaagctatcgaaatatggaatgaaatacacgcgagcttttgccaacatgtgcaatgccggggtcagcctagagaaacttgttgcagtgtctgatcaagcttgttctaagaaacatgtctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

157

Amino Acids

17.87

Weight (kDa)

9.54

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 329
AccII CGCG 1 cut(s) 385
AcsI RAATTY 1 cut(s) 32
AfiI CCNNNNNNNGG 1 cut(s) 225
AflIII ACRYGT 3 cut(s) 199, 399, 466
AgsI TTSAA 1 cut(s) 319
AluBI AGCT 9 cut(s) 75, 131, 161, 172, 232, 255, 358, 389, 452
AluI AGCT 9 cut(s) 75, 131, 161, 172, 232, 255, 358, 389, 452
Alw21I GWGCWC 2 cut(s) 133, 257
AlwNI CAGNNNCTG 1 cut(s) 443
AoxI GGCC 1 cut(s) 278
ApeKI GCWGC 1 cut(s) 169
ApoI RAATTY 1 cut(s) 32
AspS9I GGNCC 1 cut(s) 243
AsuC2I CCSGG 1 cut(s) 412
AvaII GGWCC 1 cut(s) 243
BanII GRGCYC 2 cut(s) 133, 257
Bbv12I GWGCWC 2 cut(s) 133, 257
BbvI GCAGC 1 cut(s) 181
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 1 cut(s) 412
BfaI CTAG 2 cut(s) 296, 422
BfmI CTRYAG 1 cut(s) 208
BisI GCNGC 1 cut(s) 170
BlsI GCNGC 1 cut(s) 171
Bme1390I CCNGG 1 cut(s) 412
Bme18I GGWCC 1 cut(s) 243
BmgT120I GGNCC 1 cut(s) 243
BmiI GGNNCC 1 cut(s) 244
BmrFI CCNGG 1 cut(s) 412
BmrI ACTGGG 1 cut(s) 316
BmuI ACTGGG 1 cut(s) 316
BplI GAGNNNNNCTC 2 cut(s) 239, 271
BpuEI CTTGAG 1 cut(s) 97
BpuMI CCSGG 1 cut(s) 412
BsaAI YACGTR 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 411
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse1I ACTGG 2 cut(s) 142, 311
Bse3DI GCAATG 1 cut(s) 412
BseDI CCNNGG 1 cut(s) 411
BseGI GGATG 2 cut(s) 89, 307
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMI GCAATG 1 cut(s) 412
BseNI ACTGG 2 cut(s) 142, 311
BseRI GAGGAG 1 cut(s) 265
BseXI GCAGC 1 cut(s) 181
Bsh1236I CGCG 1 cut(s) 385
BshFI GGCC 1 cut(s) 280
BsiHKAI GWGCWC 2 cut(s) 133, 257
BsiSI CCGG 1 cut(s) 411
BslFI GGGAC 2 cut(s) 256, 300
BslI CCNNNNNNNGG 1 cut(s) 225
BsmFI GGGAC 2 cut(s) 256, 300
BsnI GGCC 1 cut(s) 280
Bsp1286I GDGCHC 2 cut(s) 133, 257
Bsp143I GATC 2 cut(s) 153, 445
BspANI GGCC 1 cut(s) 280
BspFNI CGCG 1 cut(s) 385
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 1 cut(s) 244
BsrDI GCAATG 1 cut(s) 412
BsrI ACTGG 2 cut(s) 142, 311
BssECI CCNNGG 1 cut(s) 411
BssMI GATC 2 cut(s) 153, 445
BstBAI YACGTR 1 cut(s) 176
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 1 cut(s) 459
BstF5I GGATG 2 cut(s) 89, 307
BstFNI CGCG 1 cut(s) 385
BstKTI GATC 2 cut(s) 156, 448
BstMBI GATC 2 cut(s) 153, 445
BstNSI RCATGY 3 cut(s) 203, 403, 470
BstSCI CCNGG 1 cut(s) 410
BstSFI CTRYAG 1 cut(s) 208
BstSNI TACGTA 1 cut(s) 176
BstUI CGCG 1 cut(s) 385
BstV1I GCAGC 1 cut(s) 181
BsuRI GGCC 1 cut(s) 280
BtsCI GGATG 2 cut(s) 89, 307
BtsI GCAGTG 1 cut(s) 444
BtsIMutI CAGTG 1 cut(s) 444
Cac8I GCNNGC 1 cut(s) 387
CaiI CAGNNNCTG 1 cut(s) 443
CciI TCATGA 1 cut(s) 145
Cfr13I GGNCC 1 cut(s) 243
CviAII CATG 5 cut(s) 49, 146, 200, 400, 467
DdeI CTNAG 1 cut(s) 459
DpnI GATC 2 cut(s) 155, 447
DpnII GATC 2 cut(s) 153, 445
DraI TTTAAA 1 cut(s) 123
Ecl136II GAGCTC 2 cut(s) 131, 255
Eco105I TACGTA 1 cut(s) 176
Eco147I AGGCCT 1 cut(s) 280
Eco24I GRGCYC 2 cut(s) 133, 257
Eco47I GGWCC 1 cut(s) 243
Eco53kI GAGCTC 2 cut(s) 131, 255
EcoICRI GAGCTC 2 cut(s) 131, 255
EcoRI GAATTC 1 cut(s) 32
EcoT38I GRGCYC 2 cut(s) 133, 257
FaeI CATG 5 cut(s) 52, 149, 203, 403, 470
FaqI GGGAC 2 cut(s) 256, 300
FatI CATG 5 cut(s) 48, 145, 199, 399, 466
FbaI TGATCA 1 cut(s) 445
Fnu4HI GCNGC 1 cut(s) 170
FokI GGATG 2 cut(s) 76, 314
FriOI GRGCYC 2 cut(s) 133, 257
Fsp4HI GCNGC 1 cut(s) 170
FspBI CTAG 2 cut(s) 296, 422
GluI GCNGC 1 cut(s) 170
HaeIII GGCC 1 cut(s) 280
HapII CCGG 1 cut(s) 411
Hin1II CATG 5 cut(s) 52, 149, 203, 403, 470
HindIII AAGCTT 1 cut(s) 450
HinfI GANTC 3 cut(s) 19, 104, 343
HpaII CCGG 1 cut(s) 411
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 2 cut(s) 103, 445
Hpy188III TCNNGA 3 cut(s) 146, 285, 319
Hpy8I GTNNAC 1 cut(s) 205
HpyAV CCTTC 4 cut(s) 94, 108, 186, 291
HpyCH4IV ACGT 1 cut(s) 175
HpyCH4V TGCA 3 cut(s) 4, 405, 437
HpyF3I CTNAG 1 cut(s) 459
HpySE526I ACGT 1 cut(s) 175
Hsp92II CATG 5 cut(s) 52, 149, 203, 403, 470
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 2 cut(s) 153, 445
LmnI GCTCC 4 cut(s) 85, 136, 229, 252
LpnPI CCDG 4 cut(s) 123, 262, 292, 424
Lsp1109I GCAGC 1 cut(s) 181
MaeI CTAG 2 cut(s) 296, 422
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 1 cut(s) 20
MalI GATC 2 cut(s) 155, 447
MboI GATC 2 cut(s) 153, 445
MboII GAAGA 1 cut(s) 163
MhlI GDGCHC 2 cut(s) 133, 257
MluCI AATT 2 cut(s) 32, 183
MlyI GAGTC 2 cut(s) 28, 352
MmeI TCCRAC 1 cut(s) 158
MnlI CCTC 4 cut(s) 47, 103, 243, 303
MseI TTAA 1 cut(s) 122
MslI CAYNNNNRTG 2 cut(s) 47, 75
MspI CCGG 1 cut(s) 411
MspR9I CCNGG 1 cut(s) 412
MvnI CGCG 1 cut(s) 385
NciI CCSGG 1 cut(s) 412
NdeII GATC 2 cut(s) 153, 445
NlaIII CATG 5 cut(s) 52, 149, 203, 403, 470
NlaIV GGNNCC 1 cut(s) 244
NmuCI GTSAC 1 cut(s) 20
NspI RCATGY 3 cut(s) 203, 403, 470
PagI TCATGA 1 cut(s) 145
PceI AGGCCT 1 cut(s) 280
PciI ACATGT 3 cut(s) 199, 399, 466
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 1 cut(s) 171
PleI GAGTC 2 cut(s) 27, 351
PpsI GAGTC 2 cut(s) 27, 351
Ppu21I YACGTR 1 cut(s) 176
PscI ACATGT 3 cut(s) 199, 399, 466
PsiI TTATAA 1 cut(s) 329
Psp124BI GAGCTC 2 cut(s) 133, 257
PspN4I GGNNCC 1 cut(s) 244
PspPI GGNCC 1 cut(s) 243
PstNI CAGNNNCTG 1 cut(s) 443
RseI CAYNNNNRTG 2 cut(s) 47, 75
SacI GAGCTC 2 cut(s) 133, 257
SaqAI TTAA 1 cut(s) 122
SatI GCNGC 1 cut(s) 170
Sau3AI GATC 2 cut(s) 153, 445
Sau96I GGNCC 1 cut(s) 243
SchI GAGTC 2 cut(s) 28, 352
ScrFI CCNGG 1 cut(s) 412
SduI GDGCHC 2 cut(s) 133, 257
SfcI CTRYAG 1 cut(s) 208
SinI GGWCC 1 cut(s) 243
SmiMI CAYNNNNRTG 2 cut(s) 47, 75
SmlI CTYRAG 1 cut(s) 112
SmoI CTYRAG 1 cut(s) 112
SnaBI TACGTA 1 cut(s) 176
Sse9I AATT 2 cut(s) 32, 183
SseBI AGGCCT 1 cut(s) 280
SspMI CTAG 2 cut(s) 296, 422
SstI GAGCTC 2 cut(s) 133, 257
StuI AGGCCT 1 cut(s) 280
StyD4I CCNGG 1 cut(s) 410
TaiI ACGT 1 cut(s) 178
TaqI TCGA 3 cut(s) 156, 190, 362
TaqII GACCGA 1 cut(s) 260
TasI AATT 2 cut(s) 32, 183
TfiI GAWTC 1 cut(s) 104
Tru1I TTAA 1 cut(s) 122
Tru9I TTAA 1 cut(s) 122
TscAI CASTG 1 cut(s) 444
TseFI GTSAC 1 cut(s) 20
TseI GCWGC 1 cut(s) 169
Tsp45I GTSAC 1 cut(s) 20
TspDTI ATGAA 2 cut(s) 284, 389
TspRI CASTG 1 cut(s) 444
VpaK11BI GGWCC 1 cut(s) 243
XapI RAATTY 1 cut(s) 32
XceI RCATGY 3 cut(s) 203, 403, 470
XspI CTAG 2 cut(s) 296, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.