Rroxscaffold_3G00238790

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001085
Physical Location & Seq
Forward (+)
27036328 .. 27039140
2813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00238790.1

Sequence Viewer

Length: 1431 bp
ATGATCCTAATCACAAGGCCTCGCTATGTTTTACTATTCCTTGCATTACTGAGTTTGACATCCATTTCACCATCATCATCATCGAAAGTGGAAGATATCAGCAACGGAACAAGATGGGCAGTTCTGATTGCTGGGTCAAGAGGATATGATAACTATAGGCATCAGGCTGATGTATGCCATGCATACCAGATACTGAAGAAAGGTGGACTCAAAGATGAGAATATCATTGTGTTCATGTATGACGATATTGCATTCAACCCAAAAAATCCTAGACCTGGTGTCATTATCAACAAGCCAGATGGAAATGATGTTTATCAAGGAGTTCCCAAGGACTATACAGGAAAGGATATTAATACTCACAACTTCTATGCTGTTCTTCTTGGCAACATAAGTGCCCTCACTGGTGGTAGCGGCAAGGTTCTAAATAGCGGTCCAAACGACCGCGTTTTCATTTATTACGCTGACCATGGCAGCCCTGGGATGGTTGGCATGCCCATTGCAGGTGATTATGTATATGCTAAAGATCTCATAGATGTGCTGAAGAAGAAGCACGATGCAAAAGGTTACAAAAGCATGGTGTTTTACATGGAAGCTTGCGAGTCTGGGAGCCTGTTTGAGGGCATCCTTCCCAACAATATAAGCATATACGCAACTACAGCAGCAAATTCAAAAGAGAGCAGTTGGGGATTCTATTGCCCTGGAGACTTCCCTAGTCCTCCTCCAGGATATGACGTTTGCTTGGGAGACTTGTATAGTATTTCTTGGCTTGAGGATTGTGACAAGCATGACTTGAGCAAAGAAACTTTGGCGAAGCAATATGAAGTGGTCCGAAGGAGAACAGCAAATGGCTACTTAGATTCAAGCCATGTCATGCAATTTGGGAATAAGAGACAAAGCGAGGATTTCATCTTCACTTACATGGGTACAAATCCTGAAAATGATAACTATACTTACTTCAAGTACACGGCATTACCAGCGACCTCAAGGCCAAGGGTTGTCAGCCAACGCAACGCAGACCTCCTCCATCTTCGGTACAAGTTCGACAATGCCCCTGATGGCTCCGACGTGAAGCTTGAAGCTCACAAACAGCTCATTGATGAAATTTCTCGGAGGAAAAAGGTGGATCATAACATAAAGCAGATAGGGAAGCTTTTGTTTGGGTATGACAAGAGTTCAGAAGTGATGAAGAATGTTAGGACTCCGGGGGAACCTCTAGTAGATGATTGGGACTGCTTTAAGATGCTTGTGAAAACTTATGAGAAACACTGTGGATTTTTATCAAGTTATGGAAGGAAACACACGAGAGCTATTGCCAACATGTGCAATACTGGAGTTACGCCGGAACAAATGGCTGCTGCATGTGTTCAAACCTGTCATATGAAATCCCCTCTCTTAATTCCCTTGCTATACCATAAAGTGATTAATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

476

Amino Acids

53.46

Weight (kDa)

8.15

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 38 - 309 2.3e-114 Peptidase C13 family
Legum_prodom PF20985 362 - 458 8.8e-39 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 491
Acc36I ACCTGC 1 cut(s) 491
AccII CGCG 1 cut(s) 444
AciI CCGC 3 cut(s) 411, 429, 442
AclWI GGATC 1 cut(s) 1131
AcsI RAATTY 2 cut(s) 664, 1101
AcuI CTGAAG 2 cut(s) 215, 560
AfaI GTAC 3 cut(s) 925, 962, 1034
AfiI CCNNNNNNNGG 5 cut(s) 275, 481, 500, 616, 722
AflIII ACRYGT 1 cut(s) 1317
AgsI TTSAA 6 cut(s) 256, 669, 861, 958, 1076, 1367
AhdI GACNNNNNGTC 1 cut(s) 278
AjiI CACGTC 1 cut(s) 1066
AjnI CCWGG 4 cut(s) 274, 475, 697, 721
AluBI AGCT 6 cut(s) 593, 1072, 1079, 1090, 1150, 1307
AluI AGCT 6 cut(s) 593, 1072, 1079, 1090, 1150, 1307
Alw26I GTCTC 3 cut(s) 696, 738, 883
AlwI GGATC 1 cut(s) 1131
AlwNI CAGNNNCTG 1 cut(s) 193
AoxI GGCC 2 cut(s) 17, 986
ApeKI GCWGC 4 cut(s) 471, 659, 1352, 1355
ApoI RAATTY 2 cut(s) 664, 1101
AseI ATTAAT 2 cut(s) 351, 1422
AspS9I GGNCC 2 cut(s) 431, 826
AsuC2I CCSGG 1 cut(s) 1203
AsuHPI GGTGA 2 cut(s) 60, 515
AvaII GGWCC 2 cut(s) 431, 826
BaeGI GKGCMC 1 cut(s) 397
BauI CACGAG 1 cut(s) 1300
BbvI GCAGC 4 cut(s) 483, 671, 1339, 1342
BccI CCATC 6 cut(s) 79, 108, 293, 475, 1032, 1049
BceAI ACGGC 1 cut(s) 981
BciT130I CCWGG 4 cut(s) 276, 477, 699, 723
BcnI CCSGG 1 cut(s) 1203
BcoDI GTCTC 3 cut(s) 696, 738, 883
BfaI CTAG 3 cut(s) 270, 711, 1214
BfmI CTRYAG 2 cut(s) 154, 654
BfuAI ACCTGC 1 cut(s) 491
BglII AGATCT 1 cut(s) 523
BisI GCNGC 5 cut(s) 412, 472, 660, 1353, 1356
BlsI GCNGC 5 cut(s) 413, 473, 661, 1354, 1357
Bme1390I CCNGG 5 cut(s) 276, 477, 699, 723, 1203
Bme18I GGWCC 2 cut(s) 431, 826
BmeRI GACNNNNNGTC 1 cut(s) 278
BmgBI CACGTC 1 cut(s) 1066
BmgT120I GGNCC 2 cut(s) 431, 826
BmiI GGNNCC 3 cut(s) 608, 1060, 1209
BmrFI CCNGG 5 cut(s) 276, 477, 699, 723, 1203
BmsI GCATC 4 cut(s) 169, 544, 630, 1230
BpmI CTGGAG 3 cut(s) 705, 720, 1350
BpuEI CTTGAG 3 cut(s) 788, 811, 967
BpuMI CCSGG 1 cut(s) 1203
BsaBI GATNNNNATC 3 cut(s) 8, 312, 1276
BsaJI CCNNGG 7 cut(s) 327, 466, 475, 476, 697, 989, 1202
Bsc4I CCNNNNNNNGG 5 cut(s) 275, 481, 500, 616, 722
Bse1I ACTGG 2 cut(s) 406, 1333
Bse3DI GCAATG 1 cut(s) 495
Bse8I GATNNNNATC 3 cut(s) 8, 312, 1276
BseBI CCWGG 4 cut(s) 276, 477, 699, 723
BseDI CCNNGG 7 cut(s) 327, 466, 475, 476, 697, 989, 1202
BseGI GGATG 3 cut(s) 59, 486, 621
BseJI GATNNNNATC 3 cut(s) 8, 312, 1276
BseLI CCNNNNNNNGG 5 cut(s) 275, 481, 500, 616, 722
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 1 cut(s) 41
BseNI ACTGG 2 cut(s) 406, 1333
BseRI GAGGAG 2 cut(s) 708, 1010
BseSI GKGCMC 1 cut(s) 397
BseXI GCAGC 4 cut(s) 483, 671, 1339, 1342
BseYI CCCAGC 1 cut(s) 131
Bsh1236I CGCG 1 cut(s) 444
Bsh1285I CGRYCG 1 cut(s) 442
BshFI GGCC 2 cut(s) 19, 988
BsiEI CGRYCG 1 cut(s) 442
BsiSI CCGG 2 cut(s) 1202, 1340
BslFI GGGAC 1 cut(s) 1241
BslI CCNNNNNNNGG 5 cut(s) 275, 481, 500, 616, 722
BsmAI GTCTC 3 cut(s) 696, 738, 883
BsmFI GGGAC 1 cut(s) 1241
BsmI GAATGC 1 cut(s) 251
BsnI GGCC 2 cut(s) 19, 988
Bsp1286I GDGCHC 1 cut(s) 397
Bsp143I GATC 3 cut(s) 3, 523, 1123
Bsp19I CCATGG 1 cut(s) 466
BspACI CCGC 3 cut(s) 411, 429, 442
BspANI GGCC 2 cut(s) 19, 988
BspCNI CTCAG 1 cut(s) 42
BspFNI CGCG 1 cut(s) 444
BspLI GGNNCC 3 cut(s) 608, 1060, 1209
BspMI ACCTGC 1 cut(s) 491
BspPI GGATC 1 cut(s) 1131
BsrDI GCAATG 1 cut(s) 495
BsrI ACTGG 2 cut(s) 406, 1333
BssECI CCNNGG 7 cut(s) 327, 466, 475, 476, 697, 989, 1202
BssMI GATC 3 cut(s) 3, 523, 1123
BssSI CACGAG 1 cut(s) 1300
BssT1I CCWWGG 3 cut(s) 327, 466, 989
Bst2BI CACGAG 1 cut(s) 1300
Bst2UI CCWGG 4 cut(s) 276, 477, 699, 723
Bst4CI ACNGT 1 cut(s) 1268
BstC8I GCNNGC 2 cut(s) 491, 595
BstDEI CTNAG 2 cut(s) 50, 853
BstDSI CCRYGG 1 cut(s) 466
BstENI CCTNNNNNAGG 2 cut(s) 614, 720
BstF5I GGATG 3 cut(s) 59, 486, 621
BstFNI CGCG 1 cut(s) 444
BstKTI GATC 3 cut(s) 6, 526, 1126
BstMAI GTCTC 3 cut(s) 696, 738, 883
BstMBI GATC 3 cut(s) 3, 523, 1123
BstMCI CGRYCG 1 cut(s) 442
BstMWI GCNNNNNNNGC 2 cut(s) 656, 974
BstNI CCWGG 4 cut(s) 276, 477, 699, 723
BstNSI RCATGY 3 cut(s) 493, 1321, 1362
BstSCI CCNGG 5 cut(s) 274, 475, 697, 721, 1201
BstSFI CTRYAG 2 cut(s) 154, 654
BstSLI GKGCMC 1 cut(s) 397
BstUI CGCG 1 cut(s) 444
BstV1I GCAGC 4 cut(s) 483, 671, 1339, 1342
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
BsuRI GGCC 2 cut(s) 19, 988
BtgI CCRYGG 1 cut(s) 466
BtrI CACGTC 1 cut(s) 1066
BtsCI GGATG 3 cut(s) 59, 486, 621
BtsIMutI CAGTG 2 cut(s) 399, 1264
BveI ACCTGC 1 cut(s) 491
Cac8I GCNNGC 2 cut(s) 491, 595
CaiI CAGNNNCTG 1 cut(s) 193
Cfr13I GGNCC 2 cut(s) 431, 826
CsiI ACCWGGT 1 cut(s) 274
Csp6I GTAC 3 cut(s) 924, 961, 1033
CviQI GTAC 3 cut(s) 924, 961, 1033
DdeI CTNAG 2 cut(s) 50, 853
DpnI GATC 3 cut(s) 5, 525, 1125
DpnII GATC 3 cut(s) 3, 523, 1123
DriI GACNNNNNGTC 1 cut(s) 278
Eam1105I GACNNNNNGTC 1 cut(s) 278
Eco130I CCWWGG 3 cut(s) 327, 466, 989
Eco147I AGGCCT 1 cut(s) 19
Eco32I GATATC 1 cut(s) 97
Eco47I GGWCC 2 cut(s) 431, 826
Eco57I CTGAAG 2 cut(s) 215, 560
EcoNI CCTNNNNNAGG 2 cut(s) 614, 720
EcoRII CCWGG 4 cut(s) 274, 475, 697, 721
EcoRV GATATC 1 cut(s) 97
EcoT14I CCWWGG 3 cut(s) 327, 466, 989
EcoT22I ATGCAT 1 cut(s) 184
ErhI CCWWGG 3 cut(s) 327, 466, 989
FalI AAGNNNNNCTT 2 cut(s) 773, 805
FaqI GGGAC 1 cut(s) 1241
FauNDI CATATG 1 cut(s) 1377
Fnu4HI GCNGC 5 cut(s) 412, 472, 660, 1353, 1356
FokI GGATG 3 cut(s) 46, 493, 608
Fsp4HI GCNGC 5 cut(s) 412, 472, 660, 1353, 1356
FspBI CTAG 3 cut(s) 270, 711, 1214
GluI GCNGC 5 cut(s) 412, 472, 660, 1353, 1356
GsaI CCCAGC 1 cut(s) 135
GsuI CTGGAG 3 cut(s) 705, 720, 1350
HaeIII GGCC 2 cut(s) 19, 988
HapII CCGG 2 cut(s) 1202, 1340
HindIII AAGCTT 3 cut(s) 591, 1070, 1148
HinfI GANTC 5 cut(s) 207, 599, 687, 857, 1198
HpaII CCGG 2 cut(s) 1202, 1340
HphI GGTGA 2 cut(s) 60, 515
Hpy166II GTNNAC 2 cut(s) 206, 963
Hpy188I TCNGA 5 cut(s) 126, 830, 1063, 1110, 1177
Hpy188III TCNNGA 2 cut(s) 138, 932
Hpy8I GTNNAC 2 cut(s) 206, 963
Hpy99I CGWCG 1 cut(s) 1067
HpyAV CCTTC 3 cut(s) 635, 825, 1284
HpyCH4III ACNGT 1 cut(s) 1268
HpyCH4IV ACGT 2 cut(s) 732, 1065
HpyCH4V TGCA 8 cut(s) 44, 182, 251, 500, 557, 874, 1323, 1358
HpyF10VI GCNNNNNNNGC 2 cut(s) 656, 974
HpyF3I CTNAG 2 cut(s) 50, 853
HpySE526I ACGT 2 cut(s) 732, 1065
Kzo9I GATC 3 cut(s) 3, 523, 1123
LmnI GCTCC 2 cut(s) 606, 1064
Lsp1109I GCAGC 4 cut(s) 483, 671, 1339, 1342
LweI GCATC 4 cut(s) 169, 544, 630, 1230
MabI ACCWGGT 1 cut(s) 274
MaeI CTAG 3 cut(s) 270, 711, 1214
MaeII ACGT 2 cut(s) 732, 1065
MaeIII GTNAC 3 cut(s) 563, 776, 1333
MalI GATC 3 cut(s) 5, 525, 1125
MboI GATC 3 cut(s) 3, 523, 1123
MboII GAAGA 8 cut(s) 104, 208, 368, 553, 556, 901, 1019, 1198
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 1 cut(s) 397
MluCI AATT 4 cut(s) 664, 875, 1101, 1395
MlyI GAGTC 3 cut(s) 201, 608, 1192
MmeI TCCRAC 1 cut(s) 1086
Mph1103I ATGCAT 1 cut(s) 184
MseI TTAA 4 cut(s) 351, 1236, 1394, 1422
MslI CAYNNNNRTG 2 cut(s) 533, 917
MspI CCGG 2 cut(s) 1202, 1340
MspR9I CCNGG 5 cut(s) 276, 477, 699, 723, 1203
Mva1269I GAATGC 1 cut(s) 251
MvaI CCWGG 4 cut(s) 276, 477, 699, 723
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 2 cut(s) 656, 974
NciI CCSGG 1 cut(s) 1203
NcoI CCATGG 1 cut(s) 466
NdeI CATATG 1 cut(s) 1377
NdeII GATC 3 cut(s) 3, 523, 1123
NlaIV GGNNCC 3 cut(s) 608, 1060, 1209
NmuCI GTSAC 1 cut(s) 776
NsiI ATGCAT 1 cut(s) 184
NspI RCATGY 3 cut(s) 493, 1321, 1362
PaeI GCATGC 1 cut(s) 493
PaqCI CACCTGC 1 cut(s) 491
PasI CCCWGGG 1 cut(s) 476
PceI AGGCCT 1 cut(s) 19
PciI ACATGT 1 cut(s) 1317
PctI GAATGC 1 cut(s) 251
PfeI GAWTC 2 cut(s) 687, 857
PfoI TCCNGGA 1 cut(s) 721
PkrI GCNGC 5 cut(s) 413, 473, 661, 1354, 1357
PleI GAGTC 3 cut(s) 201, 607, 1192
PpsI GAGTC 3 cut(s) 201, 607, 1192
PscI ACATGT 1 cut(s) 1317
PshBI ATTAAT 2 cut(s) 351, 1422
Psp6I CCWGG 4 cut(s) 274, 475, 697, 721
PspFI CCCAGC 1 cut(s) 131
PspGI CCWGG 4 cut(s) 274, 475, 697, 721
PspN4I GGNNCC 3 cut(s) 608, 1060, 1209
PspPI GGNCC 2 cut(s) 431, 826
PstNI CAGNNNCTG 1 cut(s) 193
PsuI RGATCY 1 cut(s) 523
RsaI GTAC 3 cut(s) 925, 962, 1034
RsaNI GTAC 3 cut(s) 924, 961, 1033
RseI CAYNNNNRTG 2 cut(s) 533, 917
SaqAI TTAA 4 cut(s) 351, 1236, 1394, 1422
SatI GCNGC 5 cut(s) 412, 472, 660, 1353, 1356
Sau3AI GATC 3 cut(s) 3, 523, 1123
Sau96I GGNCC 2 cut(s) 431, 826
SchI GAGTC 3 cut(s) 201, 608, 1192
ScrFI CCNGG 5 cut(s) 276, 477, 699, 723, 1203
SduI GDGCHC 1 cut(s) 397
SexAI ACCWGGT 1 cut(s) 274
SfaNI GCATC 4 cut(s) 169, 544, 630, 1230
SfcI CTRYAG 2 cut(s) 154, 654
SinI GGWCC 2 cut(s) 431, 826
SmiMI CAYNNNNRTG 2 cut(s) 533, 917
SmlI CTYRAG 3 cut(s) 767, 790, 982
SmoI CTYRAG 3 cut(s) 767, 790, 982
SphI GCATGC 1 cut(s) 493
Sse9I AATT 4 cut(s) 664, 875, 1101, 1395
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 3 cut(s) 411, 429, 442
SspMI CTAG 3 cut(s) 270, 711, 1214
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 5 cut(s) 274, 475, 697, 721, 1201
StyI CCWWGG 3 cut(s) 327, 466, 989
TaaI ACNGT 1 cut(s) 1268
TaiI ACGT 2 cut(s) 735, 1068
TaqI TCGA 2 cut(s) 83, 1041
TasI AATT 4 cut(s) 664, 875, 1101, 1395
TatI WGTACW 1 cut(s) 960
TauI GCSGC 1 cut(s) 414
TfiI GAWTC 2 cut(s) 687, 857
Tru1I TTAA 4 cut(s) 351, 1236, 1394, 1422
Tru9I TTAA 4 cut(s) 351, 1236, 1394, 1422
TscAI CASTG 2 cut(s) 406, 1271
TseFI GTSAC 1 cut(s) 776
TseI GCWGC 4 cut(s) 471, 659, 1352, 1355
Tsp45I GTSAC 1 cut(s) 776
TspDTI ATGAA 7 cut(s) 223, 439, 834, 895, 1113, 1199, 1394
TspGWI ACGGA 1 cut(s) 120
TspRI CASTG 2 cut(s) 406, 1271
VpaK11BI GGWCC 2 cut(s) 431, 826
VspI ATTAAT 2 cut(s) 351, 1422
XagI CCTNNNNNAGG 2 cut(s) 614, 720
XapI RAATTY 2 cut(s) 664, 1101
XceI RCATGY 3 cut(s) 493, 1321, 1362
XcmI CCANNNNNNNNNTGG 1 cut(s) 473
XspI CTAG 3 cut(s) 270, 711, 1214
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.