FvH4_6g36640

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001085
Physical Location & Seq
Reverse (-)
28827040 .. 28828890
1851 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g36640.t1

Sequence Viewer

Length: 909 bp
ATGGTTGGCATGCCGATTGTAGGTGATTATGTCTATGCCAAAGATCTCATAGATGTGCTGAAGAAGAAGCACGACGCAAGAGGTTACAAAAGCATGGTGTTTTACATGGAAGCTTGCGACTCAGGGAGCATGTTTGAGGGTCTCCTTCCCAACAATATAAACATATATGCAACTTCAGCGTCAAATTCAAAAGAGAGCAGTTGGGGGTTCTATTGTCCTGGAGACTTCCCTAGTCCTCCTCCAGGATATGATGTTTGCTTGGGAGACTTGTATAATATTTCTTGGTTGGAGGATTGTGACGAGCATGACTTGAGCAAAGAAACTTTTGCGAAGCAATACGAAGTGGTACGAAGGAGAACTGCAGCAAATGGCTACTTAGATTCGAGCCATGTTATGCAATTTGGGAATAAAAGACTAAGCGAGGAATTCATCTTCACTTACATGGGTACAAATCCTGAAAATGATAACTATACTTATCTCGAGTACACTGCATCACCATCGACCTCAAGGCCAAGGGTTGTCAGCCAACGCAACGCAGACCTCCTCCATTTTCGTTACAAGCAGTTCCACAATGCTCCTGATGGCTCCGATGCCAAGCTTGAAGCTCACAAAGAGCTCGTGGATGAAATTTCTCGGAGGAAGAAGGTGGATTATCATATGAAGCAAATTGGGAAGCTTTTGTTTGGGTATGACAAGAGCTCAAAAGTGCTGACGAATGTTAGACCTCCGGGGGAACCTCTAGTTGATGATTGGGACTGCTTTAAGATGCTGGTGAAAACTTTTGAGAAACACTGCGGATTTTTATCAAACTATGGAAGGAAACACACAAGAGCAATTGCCAACATGTGCAATGCCGGAGCTACGCCGGAACAAATGGCTGCCGCATGTGTTCAAACCTGTCATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

303

Amino Acids

34.3

Weight (kDa)

6.37

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 2 - 150 3.9e-45 Peptidase C13 family
Legum_prodom PF20985 204 - 300 1.7e-38 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 795, 882
AcsI RAATTY 3 cut(s) 184, 425, 627
AcuI CTGAAG 2 cut(s) 80, 159
AfaI GTAC 3 cut(s) 348, 448, 485
AfiI CCNNNNNNNGG 2 cut(s) 20, 242
AflIII ACRYGT 1 cut(s) 843
AgsI TTSAA 3 cut(s) 189, 602, 893
AjnI CCWGG 2 cut(s) 217, 241
AluBI AGCT 7 cut(s) 113, 598, 605, 616, 676, 699, 860
AluI AGCT 7 cut(s) 113, 598, 605, 616, 676, 699, 860
Alw21I GWGCWC 2 cut(s) 618, 701
Alw26I GTCTC 3 cut(s) 146, 216, 258
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 1 cut(s) 509
ApeKI GCWGC 2 cut(s) 362, 878
ApoI RAATTY 3 cut(s) 184, 425, 627
AsuC2I CCSGG 1 cut(s) 729
AsuHPI GGTGA 3 cut(s) 35, 486, 784
AvaI CYCGRG 1 cut(s) 479
BanII GRGCYC 2 cut(s) 618, 701
BauI CACGAG 1 cut(s) 617
Bbv12I GWGCWC 2 cut(s) 618, 701
BbvI GCAGC 2 cut(s) 374, 865
BccI CCATC 2 cut(s) 505, 575
BcgI CGANNNNNNTGC 4 cut(s) 470, 480, 504, 514
BciT130I CCWGG 2 cut(s) 219, 243
BcnI CCSGG 1 cut(s) 729
BcoDI GTCTC 3 cut(s) 146, 216, 258
BfaI CTAG 2 cut(s) 231, 740
BfmI CTRYAG 1 cut(s) 360
BglII AGATCT 1 cut(s) 43
BisI GCNGC 3 cut(s) 363, 879, 882
BlsI GCNGC 3 cut(s) 364, 880, 883
Bme1390I CCNGG 3 cut(s) 219, 243, 729
BmeT110I CYCGRG 1 cut(s) 479
BmiI GGNNCC 2 cut(s) 586, 735
BmrFI CCNGG 3 cut(s) 219, 243, 729
BmsI GCATC 3 cut(s) 500, 580, 756
BpmI CTGGAG 2 cut(s) 225, 240
BpuEI CTTGAG 2 cut(s) 331, 490
BpuMI CCSGG 1 cut(s) 729
BsaBI GATNNNNATC 1 cut(s) 802
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 2 cut(s) 512, 728
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 242
Bse3DI GCAATG 1 cut(s) 856
Bse8I GATNNNNATC 1 cut(s) 802
BseBI CCWGG 2 cut(s) 219, 243
BseDI CCNNGG 2 cut(s) 512, 728
BseGI GGATG 1 cut(s) 628
BseJI GATNNNNATC 1 cut(s) 802
BseLI CCNNNNNNNGG 2 cut(s) 20, 242
BseMI GCAATG 1 cut(s) 856
BseMII CTCAG 1 cut(s) 135
BseRI GAGGAG 2 cut(s) 228, 533
BseXI GCAGC 2 cut(s) 374, 865
BshFI GGCC 1 cut(s) 511
BsiHKAI GWGCWC 2 cut(s) 618, 701
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 3 cut(s) 728, 855, 866
BslFI GGGAC 1 cut(s) 767
BslI CCNNNNNNNGG 2 cut(s) 20, 242
BsmAI GTCTC 3 cut(s) 146, 216, 258
BsmFI GGGAC 1 cut(s) 767
BsnI GGCC 1 cut(s) 511
Bso31I GGTCTC 1 cut(s) 146
BsoBI CYCGRG 1 cut(s) 479
Bsp1286I GDGCHC 2 cut(s) 618, 701
Bsp143I GATC 1 cut(s) 43
BspACI CCGC 2 cut(s) 795, 882
BspANI GGCC 1 cut(s) 511
BspCNI CTCAG 1 cut(s) 134
BspLI GGNNCC 2 cut(s) 586, 735
BspMAI CTGCAG 1 cut(s) 364
BspTNI GGTCTC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 856
BssECI CCNNGG 2 cut(s) 512, 728
BssMI GATC 1 cut(s) 43
BssSI CACGAG 1 cut(s) 617
BssT1I CCWWGG 1 cut(s) 512
Bst2BI CACGAG 1 cut(s) 617
Bst2UI CCWGG 2 cut(s) 219, 243
BstC8I GCNNGC 2 cut(s) 11, 115
BstDEI CTNAG 3 cut(s) 121, 376, 416
BstENI CCTNNNNNAGG 1 cut(s) 240
BstF5I GGATG 1 cut(s) 628
BstKTI GATC 1 cut(s) 46
BstMAI GTCTC 3 cut(s) 146, 216, 258
BstMBI GATC 1 cut(s) 43
BstMWI GCNNNNNNNGC 1 cut(s) 176
BstNI CCWGG 2 cut(s) 219, 243
BstNSI RCATGY 4 cut(s) 13, 133, 847, 888
BstSCI CCNGG 3 cut(s) 217, 241, 727
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 2 cut(s) 374, 865
BstX2I RGATCY 1 cut(s) 43
BstYI RGATCY 1 cut(s) 43
BsuRI GGCC 1 cut(s) 511
BtsCI GGATG 1 cut(s) 628
BtsI GCAGTG 2 cut(s) 486, 790
BtsIMutI CAGTG 2 cut(s) 486, 790
Cac8I GCNNGC 2 cut(s) 11, 115
CseI GACGC 2 cut(s) 83, 168
Csp6I GTAC 3 cut(s) 347, 447, 484
CviQI GTAC 3 cut(s) 347, 447, 484
DdeI CTNAG 3 cut(s) 121, 376, 416
DpnI GATC 1 cut(s) 45
DpnII GATC 1 cut(s) 43
Ecl136II GAGCTC 2 cut(s) 616, 699
Eco130I CCWWGG 1 cut(s) 512
Eco24I GRGCYC 2 cut(s) 618, 701
Eco31I GGTCTC 1 cut(s) 146
Eco53kI GAGCTC 2 cut(s) 616, 699
Eco57I CTGAAG 2 cut(s) 80, 159
Eco88I CYCGRG 1 cut(s) 479
EcoICRI GAGCTC 2 cut(s) 616, 699
EcoNI CCTNNNNNAGG 1 cut(s) 240
EcoRI GAATTC 1 cut(s) 425
EcoRII CCWGG 2 cut(s) 217, 241
EcoT14I CCWWGG 1 cut(s) 512
EcoT38I GRGCYC 2 cut(s) 618, 701
ErhI CCWWGG 1 cut(s) 512
FaqI GGGAC 1 cut(s) 767
FauNDI CATATG 1 cut(s) 657
Fnu4HI GCNGC 3 cut(s) 363, 879, 882
FokI GGATG 1 cut(s) 635
FriOI GRGCYC 2 cut(s) 618, 701
Fsp4HI GCNGC 3 cut(s) 363, 879, 882
FspBI CTAG 2 cut(s) 231, 740
GluI GCNGC 3 cut(s) 363, 879, 882
GsuI CTGGAG 2 cut(s) 225, 240
HaeIII GGCC 1 cut(s) 511
HapII CCGG 3 cut(s) 728, 855, 866
HgaI GACGC 2 cut(s) 83, 168
HindIII AAGCTT 3 cut(s) 111, 596, 674
HinfI GANTC 2 cut(s) 119, 380
HpaII CCGG 3 cut(s) 728, 855, 866
HphI GGTGA 3 cut(s) 35, 486, 784
Hpy166II GTNNAC 1 cut(s) 486
Hpy188I TCNGA 2 cut(s) 589, 636
Hpy188III TCNNGA 3 cut(s) 455, 479, 578
Hpy8I GTNNAC 1 cut(s) 486
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 4 cut(s) 155, 345, 637, 810
HpyCH4V TGCA 5 cut(s) 170, 362, 397, 491, 849
HpyF10VI GCNNNNNNNGC 1 cut(s) 176
HpyF3I CTNAG 3 cut(s) 121, 376, 416
Kzo9I GATC 1 cut(s) 43
LmnI GCTCC 4 cut(s) 126, 580, 590, 857
Lsp1109I GCAGC 2 cut(s) 374, 865
LweI GCATC 3 cut(s) 500, 580, 756
MaeI CTAG 2 cut(s) 231, 740
MaeIII GTNAC 3 cut(s) 83, 296, 554
MalI GATC 1 cut(s) 45
MboI GATC 1 cut(s) 43
MboII GAAGA 4 cut(s) 73, 76, 424, 652
MfeI CAATTG 1 cut(s) 834
MflI RGATCY 1 cut(s) 43
MhlI GDGCHC 2 cut(s) 618, 701
MluCI AATT 6 cut(s) 184, 398, 425, 627, 666, 834
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 1 cut(s) 267
MseI TTAA 2 cut(s) 762, 907
MslI CAYNNNNRTG 2 cut(s) 53, 440
MspI CCGG 3 cut(s) 728, 855, 866
MspR9I CCNGG 3 cut(s) 219, 243, 729
MunI CAATTG 1 cut(s) 834
MvaI CCWGG 2 cut(s) 219, 243
MwoI GCNNNNNNNGC 1 cut(s) 176
NciI CCSGG 1 cut(s) 729
NdeI CATATG 1 cut(s) 657
NdeII GATC 1 cut(s) 43
NlaIV GGNNCC 2 cut(s) 586, 735
NmuCI GTSAC 1 cut(s) 296
NspI RCATGY 4 cut(s) 13, 133, 847, 888
PaeI GCATGC 1 cut(s) 13
PaeR7I CTCGAG 1 cut(s) 479
PciI ACATGT 1 cut(s) 843
PfeI GAWTC 1 cut(s) 380
PfoI TCCNGGA 2 cut(s) 217, 241
PkrI GCNGC 3 cut(s) 364, 880, 883
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
PscI ACATGT 1 cut(s) 843
Psp124BI GAGCTC 2 cut(s) 618, 701
Psp6I CCWGG 2 cut(s) 217, 241
PspGI CCWGG 2 cut(s) 217, 241
PspN4I GGNNCC 2 cut(s) 586, 735
PstI CTGCAG 1 cut(s) 364
PsuI RGATCY 1 cut(s) 43
RsaI GTAC 3 cut(s) 348, 448, 485
RsaNI GTAC 3 cut(s) 347, 447, 484
RseI CAYNNNNRTG 2 cut(s) 53, 440
SacI GAGCTC 2 cut(s) 618, 701
SaqAI TTAA 2 cut(s) 762, 907
SatI GCNGC 3 cut(s) 363, 879, 882
Sau3AI GATC 1 cut(s) 43
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 3 cut(s) 219, 243, 729
SduI GDGCHC 2 cut(s) 618, 701
SfaNI GCATC 3 cut(s) 500, 580, 756
SfcI CTRYAG 1 cut(s) 360
Sfr274I CTCGAG 1 cut(s) 479
SlaI CTCGAG 1 cut(s) 479
SmiMI CAYNNNNRTG 2 cut(s) 53, 440
SmlI CTYRAG 3 cut(s) 310, 479, 505
SmoI CTYRAG 3 cut(s) 310, 479, 505
SphI GCATGC 1 cut(s) 13
Sse9I AATT 6 cut(s) 184, 398, 425, 627, 666, 834
SsiI CCGC 2 cut(s) 795, 882
SspI AATATT 1 cut(s) 277
SspMI CTAG 2 cut(s) 231, 740
SstI GAGCTC 2 cut(s) 618, 701
StyD4I CCNGG 3 cut(s) 217, 241, 727
StyI CCWWGG 1 cut(s) 512
TaqI TCGA 3 cut(s) 383, 480, 500
TasI AATT 6 cut(s) 184, 398, 425, 627, 666, 834
TatI WGTACW 1 cut(s) 483
TauI GCSGC 1 cut(s) 884
TfiI GAWTC 1 cut(s) 380
Tru1I TTAA 2 cut(s) 762, 907
Tru9I TTAA 2 cut(s) 762, 907
TscAI CASTG 2 cut(s) 493, 797
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 2 cut(s) 362, 878
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 3 cut(s) 418, 639, 674
TspRI CASTG 2 cut(s) 493, 797
XagI CCTNNNNNAGG 1 cut(s) 240
XapI RAATTY 3 cut(s) 184, 425, 627
XceI RCATGY 4 cut(s) 13, 133, 847, 888
XhoI CTCGAG 1 cut(s) 479
XspI CTAG 2 cut(s) 231, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.