MD04G1024700.v1.1

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001085
Physical Location & Seq
Reverse (-)
2855660 .. 2857857
2198 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1024700.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGGCTAATCATGGTTATTGTGGGGTTTTACTATCTCTTACTTTGTTAAGTTTGGCTATTCATGGAAGCTTCTGCTTCCCTGAAATTAATGGAGATAACAAGGGTTCTCCACGTACCACTACTGATAAGGGAAAAAGATGGGCAGTTCTGGTTGCAGGATCAAGTGGTTACGACAACTACAGGCACCAGGCTGACATATGCCATGCGTACCAGATACTTAAAAAAGGAGGACTGAAAGACGAGAACATCATCGTTTTCATGTACGATGATATCGCGTACAACTCGGAAAATCCTCGAAAAGGTGTCATCATCAACAAGCCAAATGGTCATGATGTTTATAAAGGAGTTCCCAAGGATTATACAGGAGATCATGTTAACGCACGCAATCTCTATGCTGTTATTCTCGGAGACAAAAGTGCTCTCACCGGAGGAAGTGGCAAGGTTCTAAGTAGCGGTCCGAATGACCATGTTTTCATATATTATGCAGACCCTGGTTCTGTATTTTACATTGAAGCTTGCGAGGCAGGGAGCATGTTTGAGGGCCTCCTTTCAAGTAATCTGAATATTTATGCTACCACTGCGTCGAATGCAGAAGAGAGTAGGTATGGAACATATTGTCCCGGTGACCCATCAGTTCCTAACGAGTTTGATACTTGTTTGGGAGACTTGTATAGCATTTCCTGGATGGAGGCTTGCAATGACATAAGTGATTTGCATAAAGAAACTTTGGAGAATCAATATGAAAGGGTTCGAAGCAGAACAACCAATTCGAATGTTATGCAGTACGGAGATATGAGTCATAAACAGGAGTTCCTCTTTGCTTACATGGGTGCAGATCTTTCTAATCGTAGCCATACTTCCACCAGCGATATCTCTTCACCCTCGATCTCAAGGGCTGTTGACCAACGTGACACGAAGCTCCTCTACTTTCAACAAAAGCTGCAAAGAGCTCCTACCGGATCTCAAGAGAAACAGGGTGCTCAGAAGCAGCTGCTGCGTGAAATTGCTCGTAGGAAAAACGTGGACTATAGCATAACGAAACTAGGGGAGGCTTTGTTCGGACACGAGAAGAGCTCAAACGTTTTAATGAATGTTAGGCCACAGGGCCAACCTGTGGTAGATAATTGGGACTGCTTCAAGAACTATGTAAGTTCATCTCCTGTAATTCTATACATCCAATTGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

396

Amino Acids

43.88

Weight (kDa)

6.13

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 46 - 166 6.1e-57 Peptidase C13 family
Peptidase_C13 PF01650 167 - 277 2.4e-35 Peptidase C13 family
Legum_prodom PF20985 329 - 384 1.2e-14 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 183
AccB7I CCANNNNNTGG 1 cut(s) 1114
AccII CGCG 1 cut(s) 275
AciI CCGC 1 cut(s) 453
AclI AACGTT 1 cut(s) 1080
AclWI GGATC 2 cut(s) 166, 967
AfaI GTAC 5 cut(s) 115, 209, 263, 278, 785
AfiI CCNNNNNNNGG 2 cut(s) 299, 1114
AgsI TTSAA 4 cut(s) 512, 552, 932, 1138
AjnI CCWGG 3 cut(s) 186, 490, 680
AloI GAACNNNNNNTCC 4 cut(s) 601, 633, 1040, 1072
AluBI AGCT 7 cut(s) 69, 515, 919, 940, 950, 991, 1074
AluI AGCT 7 cut(s) 69, 515, 919, 940, 950, 991, 1074
Alw21I GWGCWC 4 cut(s) 421, 952, 982, 1076
Alw26I GTCTC 2 cut(s) 402, 657
AlwI GGATC 2 cut(s) 166, 967
AlwNI CAGNNNCTG 2 cut(s) 491, 994
AoxI GGCC 3 cut(s) 541, 1097, 1105
ApeKI GCWGC 4 cut(s) 940, 988, 991, 994
AseI ATTAAT 1 cut(s) 87
Asp700I GAANNNNTTC 1 cut(s) 747
AspS9I GGNCC 3 cut(s) 455, 541, 1105
AsuC2I CCSGG 1 cut(s) 621
AsuHPI GGTGA 3 cut(s) 415, 635, 870
AsuII TTCGAA 2 cut(s) 751, 770
AvaII GGWCC 1 cut(s) 455
BanI GGYRCC 1 cut(s) 183
BanII GRGCYC 2 cut(s) 952, 1076
BarI GAAGNNNNNNTAC 4 cut(s) 841, 873, 908, 940
BauI CACGAG 1 cut(s) 1064
Bbv12I GWGCWC 4 cut(s) 421, 952, 982, 1076
BbvI GCAGC 4 cut(s) 927, 978, 981, 1000
BccI CCATC 3 cut(s) 132, 637, 679
BcgI CGANNNNNNTGC 2 cut(s) 760, 794
BciT130I CCWGG 3 cut(s) 188, 492, 682
BcnI CCSGG 1 cut(s) 621
BcoDI GTCTC 2 cut(s) 402, 657
BfaI CTAG 2 cut(s) 1043, 1186
BfmI CTRYAG 2 cut(s) 178, 1027
BglII AGATCT 1 cut(s) 835
BisI GCNGC 4 cut(s) 941, 989, 992, 995
BlsI GCNGC 4 cut(s) 942, 990, 993, 996
Bme1390I CCNGG 4 cut(s) 188, 492, 621, 682
Bme18I GGWCC 1 cut(s) 455
BmgT120I GGNCC 3 cut(s) 455, 541, 1105
BmiI GGNNCC 1 cut(s) 185
BmrFI CCNGG 4 cut(s) 188, 492, 621, 682
BplI GAGNNNNNCTC 2 cut(s) 1058, 1090
Bpu14I TTCGAA 2 cut(s) 751, 770
BpuEI CTTGAG 2 cut(s) 874, 948
BpuMI CCSGG 1 cut(s) 621
BsaAI YACGTR 1 cut(s) 113
BsaJI CCNNGG 2 cut(s) 351, 490
BsaWI WCCGGW 2 cut(s) 425, 956
BsaXI ACNNNNNCTCC 4 cut(s) 357, 387, 1040, 1070
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 1114
Bse3DI GCAATG 1 cut(s) 703
BseBI CCWGG 3 cut(s) 188, 492, 682
BseDI CCNNGG 2 cut(s) 351, 490
BseGI GGATG 2 cut(s) 690, 1173
BseLI CCNNNNNNNGG 2 cut(s) 299, 1114
BseMI GCAATG 1 cut(s) 703
BseMII CTCAG 1 cut(s) 995
BseRI GAGGAG 1 cut(s) 911
BseXI GCAGC 4 cut(s) 927, 978, 981, 1000
BsgI GTGCAG 1 cut(s) 852
Bsh1236I CGCG 1 cut(s) 275
BshFI GGCC 3 cut(s) 543, 1099, 1107
BshNI GGYRCC 1 cut(s) 183
BsiHKAI GWGCWC 4 cut(s) 421, 952, 982, 1076
BsiSI CCGG 3 cut(s) 426, 621, 957
BslFI GGGAC 2 cut(s) 603, 1142
BslI CCNNNNNNNGG 2 cut(s) 299, 1114
BsmAI GTCTC 2 cut(s) 402, 657
BsmFI GGGAC 2 cut(s) 603, 1142
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 3 cut(s) 543, 1099, 1107
Bsp119I TTCGAA 2 cut(s) 751, 770
Bsp1286I GDGCHC 4 cut(s) 421, 952, 982, 1076
Bsp143I GATC 6 cut(s) 158, 367, 835, 885, 959, 1182
BspACI CCGC 1 cut(s) 453
BspANI GGCC 3 cut(s) 543, 1099, 1107
BspCNI CTCAG 1 cut(s) 994
BspFNI CGCG 1 cut(s) 275
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 1 cut(s) 185
BspPI GGATC 2 cut(s) 166, 967
BspQI GCTCTTC 1 cut(s) 1064
BspT104I TTCGAA 2 cut(s) 751, 770
BspT107I GGYRCC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 703
BssECI CCNNGG 2 cut(s) 351, 490
BssMI GATC 6 cut(s) 158, 367, 835, 885, 959, 1182
BssSI CACGAG 1 cut(s) 1064
BssT1I CCWWGG 1 cut(s) 351
Bst2BI CACGAG 1 cut(s) 1064
Bst2UI CCWGG 3 cut(s) 188, 492, 682
Bst6I CTCTTC 3 cut(s) 588, 880, 1064
BstAPI GCANNNNNTGC 1 cut(s) 994
BstBAI YACGTR 1 cut(s) 113
BstBI TTCGAA 2 cut(s) 751, 770
BstC8I GCNNGC 3 cut(s) 382, 517, 694
BstDEI CTNAG 2 cut(s) 446, 981
BstEII GGTNACC 1 cut(s) 623
BstENI CCTNNNNNAGG 1 cut(s) 297
BstF5I GGATG 2 cut(s) 690, 1173
BstFNI CGCG 1 cut(s) 275
BstKTI GATC 6 cut(s) 161, 370, 838, 888, 962, 1185
BstMAI GTCTC 2 cut(s) 402, 657
BstMBI GATC 6 cut(s) 158, 367, 835, 885, 959, 1182
BstMWI GCNNNNNNNGC 4 cut(s) 521, 578, 587, 994
BstNI CCWGG 3 cut(s) 188, 492, 682
BstNSI RCATGY 1 cut(s) 535
BstPI GGTNACC 1 cut(s) 623
BstSCI CCNGG 4 cut(s) 186, 490, 619, 680
BstSFI CTRYAG 2 cut(s) 178, 1027
BstUI CGCG 1 cut(s) 275
BstV1I GCAGC 4 cut(s) 927, 978, 981, 1000
BstX2I RGATCY 2 cut(s) 835, 959
BstYI RGATCY 2 cut(s) 835, 959
BsuRI GGCC 3 cut(s) 543, 1099, 1107
BtsCI GGATG 2 cut(s) 690, 1173
BtsI GCAGTG 1 cut(s) 576
BtsIMutI CAGTG 1 cut(s) 576
Cac8I GCNNGC 3 cut(s) 382, 517, 694
CaiI CAGNNNCTG 2 cut(s) 491, 994
CciI TCATGA 1 cut(s) 328
Cfr13I GGNCC 3 cut(s) 455, 541, 1105
CpoI CGGWCCG 1 cut(s) 455
CseI GACGC 1 cut(s) 570
Csp6I GTAC 5 cut(s) 114, 208, 262, 277, 784
CspI CGGWCCG 1 cut(s) 455
CviAII CATG 9 cut(s) 11, 62, 203, 259, 329, 371, 467, 532, 826
CviQI GTAC 5 cut(s) 114, 208, 262, 277, 784
DdeI CTNAG 2 cut(s) 446, 981
DpnI GATC 6 cut(s) 160, 369, 837, 887, 961, 1184
DpnII GATC 6 cut(s) 158, 367, 835, 885, 959, 1182
Eam1104I CTCTTC 3 cut(s) 588, 880, 1064
EarI CTCTTC 3 cut(s) 588, 880, 1064
Ecl136II GAGCTC 2 cut(s) 950, 1074
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 2 cut(s) 952, 1076
Eco32I GATATC 2 cut(s) 271, 871
Eco47I GGWCC 1 cut(s) 455
Eco53kI GAGCTC 2 cut(s) 950, 1074
Eco91I GGTNACC 1 cut(s) 623
EcoICRI GAGCTC 2 cut(s) 950, 1074
EcoNI CCTNNNNNAGG 1 cut(s) 297
EcoO109I RGGNCCY 1 cut(s) 541
EcoO65I GGTNACC 1 cut(s) 623
EcoRII CCWGG 3 cut(s) 186, 490, 680
EcoRV GATATC 2 cut(s) 271, 871
EcoT14I CCWWGG 1 cut(s) 351
EcoT38I GRGCYC 2 cut(s) 952, 1076
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 9 cut(s) 14, 65, 206, 262, 332, 374, 470, 535, 829
FaqI GGGAC 2 cut(s) 603, 1142
FatI CATG 9 cut(s) 10, 61, 202, 258, 328, 370, 466, 531, 825
FauNDI CATATG 1 cut(s) 197
Fnu4HI GCNGC 4 cut(s) 941, 989, 992, 995
FokI GGATG 2 cut(s) 697, 1160
FriOI GRGCYC 2 cut(s) 952, 1076
Fsp4HI GCNGC 4 cut(s) 941, 989, 992, 995
FspBI CTAG 2 cut(s) 1043, 1186
GluI GCNGC 4 cut(s) 941, 989, 992, 995
HaeIII GGCC 3 cut(s) 543, 1099, 1107
HapII CCGG 3 cut(s) 426, 621, 957
HgaI GACGC 1 cut(s) 570
Hin1II CATG 9 cut(s) 14, 65, 206, 262, 332, 374, 470, 535, 829
HincII GTYRAC 2 cut(s) 376, 901
HindII GTYRAC 2 cut(s) 376, 901
HindIII AAGCTT 2 cut(s) 67, 513
HinfI GANTC 2 cut(s) 733, 796
HpaI GTTAAC 1 cut(s) 376
HpaII CCGG 3 cut(s) 426, 621, 957
HphI GGTGA 3 cut(s) 415, 635, 870
Hpy166II GTNNAC 3 cut(s) 376, 901, 1024
Hpy188I TCNGA 6 cut(s) 286, 407, 459, 561, 984, 1061
Hpy188III TCNNGA 3 cut(s) 329, 965, 1138
Hpy8I GTNNAC 3 cut(s) 376, 901, 1024
Hpy99I CGWCG 1 cut(s) 586
HpyCH4IV ACGT 4 cut(s) 112, 907, 1020, 1080
HpyCH4V TGCA 8 cut(s) 155, 485, 590, 696, 715, 781, 833, 943
HpyF10VI GCNNNNNNNGC 4 cut(s) 521, 578, 587, 994
HpyF3I CTNAG 2 cut(s) 446, 981
HpySE526I ACGT 4 cut(s) 112, 907, 1020, 1080
Hsp92II CATG 9 cut(s) 14, 65, 206, 262, 332, 374, 470, 535, 829
KspAI GTTAAC 1 cut(s) 376
Kzo9I GATC 6 cut(s) 158, 367, 835, 885, 959, 1182
LguI GCTCTTC 1 cut(s) 1064
LmnI GCTCC 3 cut(s) 528, 924, 955
Lsp1109I GCAGC 4 cut(s) 927, 978, 981, 1000
MaeI CTAG 2 cut(s) 1043, 1186
MaeII ACGT 4 cut(s) 112, 907, 1020, 1080
MaeIII GTNAC 3 cut(s) 167, 623, 908
MalI GATC 6 cut(s) 160, 369, 837, 887, 961, 1184
MboI GATC 6 cut(s) 158, 367, 835, 885, 959, 1182
MboII GAAGA 3 cut(s) 605, 867, 1081
MfeI CAATTG 1 cut(s) 1178
MflI RGATCY 2 cut(s) 835, 959
MhlI GDGCHC 4 cut(s) 421, 952, 982, 1076
MluCI AATT 6 cut(s) 84, 766, 1002, 1123, 1164, 1178
MlyI GAGTC 1 cut(s) 805
MroXI GAANNNNTTC 1 cut(s) 747
MseI TTAA 5 cut(s) 47, 87, 219, 375, 1085
MspA1I CMGCKG 1 cut(s) 991
MspI CCGG 3 cut(s) 426, 621, 957
MspR9I CCNGG 4 cut(s) 188, 492, 621, 682
MunI CAATTG 1 cut(s) 1178
Mva1269I GAATGC 1 cut(s) 592
MvaI CCWGG 3 cut(s) 188, 492, 682
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 4 cut(s) 521, 578, 587, 994
NciI CCSGG 1 cut(s) 621
NdeI CATATG 1 cut(s) 197
NdeII GATC 6 cut(s) 158, 367, 835, 885, 959, 1182
NlaIII CATG 9 cut(s) 14, 65, 206, 262, 332, 374, 470, 535, 829
NlaIV GGNNCC 1 cut(s) 185
NmuCI GTSAC 2 cut(s) 623, 908
NspI RCATGY 1 cut(s) 535
NspV TTCGAA 2 cut(s) 751, 770
PagI TCATGA 1 cut(s) 328
PciSI GCTCTTC 1 cut(s) 1064
PctI GAATGC 1 cut(s) 592
PdmI GAANNNNTTC 1 cut(s) 747
PfeI GAWTC 1 cut(s) 733
PflMI CCANNNNNTGG 1 cut(s) 1114
PfoI TCCNGGA 1 cut(s) 680
PkrI GCNGC 4 cut(s) 942, 990, 993, 996
PleI GAGTC 1 cut(s) 804
PpsI GAGTC 1 cut(s) 804
Ppu21I YACGTR 1 cut(s) 113
PshBI ATTAAT 1 cut(s) 87
PsiI TTATAA 1 cut(s) 339
Psp124BI GAGCTC 2 cut(s) 952, 1076
Psp1406I AACGTT 1 cut(s) 1080
Psp6I CCWGG 3 cut(s) 186, 490, 680
PspEI GGTNACC 1 cut(s) 623
PspGI CCWGG 3 cut(s) 186, 490, 680
PspN4I GGNNCC 1 cut(s) 185
PspPI GGNCC 3 cut(s) 455, 541, 1105
PstNI CAGNNNCTG 2 cut(s) 491, 994
PsuI RGATCY 2 cut(s) 835, 959
PvuII CAGCTG 1 cut(s) 991
RsaI GTAC 5 cut(s) 115, 209, 263, 278, 785
RsaNI GTAC 5 cut(s) 114, 208, 262, 277, 784
Rsr2I CGGWCCG 1 cut(s) 455
RsrII CGGWCCG 1 cut(s) 455
SacI GAGCTC 2 cut(s) 952, 1076
SapI GCTCTTC 1 cut(s) 1064
SaqAI TTAA 5 cut(s) 47, 87, 219, 375, 1085
SatI GCNGC 4 cut(s) 941, 989, 992, 995
Sau3AI GATC 6 cut(s) 158, 367, 835, 885, 959, 1182
Sau96I GGNCC 3 cut(s) 455, 541, 1105
SchI GAGTC 1 cut(s) 805
ScrFI CCNGG 4 cut(s) 188, 492, 621, 682
SduI GDGCHC 4 cut(s) 421, 952, 982, 1076
SfcI CTRYAG 2 cut(s) 178, 1027
SfuI TTCGAA 2 cut(s) 751, 770
SinI GGWCC 1 cut(s) 455
SmlI CTYRAG 2 cut(s) 889, 963
SmoI CTYRAG 2 cut(s) 889, 963
Sse9I AATT 6 cut(s) 84, 766, 1002, 1123, 1164, 1178
SsiI CCGC 1 cut(s) 453
SspI AATATT 1 cut(s) 565
SspMI CTAG 2 cut(s) 1043, 1186
SstI GAGCTC 2 cut(s) 952, 1076
StyD4I CCNGG 4 cut(s) 186, 490, 619, 680
StyI CCWWGG 1 cut(s) 351
TaiI ACGT 4 cut(s) 115, 910, 1023, 1083
TaqI TCGA 5 cut(s) 295, 584, 751, 770, 884
TasI AATT 6 cut(s) 84, 766, 1002, 1123, 1164, 1178
TfiI GAWTC 1 cut(s) 733
Tru1I TTAA 5 cut(s) 47, 87, 219, 375, 1085
Tru9I TTAA 5 cut(s) 47, 87, 219, 375, 1085
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 2 cut(s) 623, 908
TseI GCWGC 4 cut(s) 940, 988, 991, 994
Tsp45I GTSAC 2 cut(s) 623, 908
TspDTI ATGAA 6 cut(s) 50, 247, 463, 756, 1103, 1143
TspGWI ACGGA 1 cut(s) 801
TspRI CASTG 1 cut(s) 583
Van91I CCANNNNNTGG 1 cut(s) 1114
VpaK11BI GGWCC 1 cut(s) 455
VspI ATTAAT 1 cut(s) 87
XagI CCTNNNNNAGG 1 cut(s) 297
XceI RCATGY 1 cut(s) 535
XmnI GAANNNNTTC 1 cut(s) 747
XspI CTAG 2 cut(s) 1043, 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.