Rh2BG481400

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001085
Physical Location & Seq
Reverse (-)
68107199 .. 68109531
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG481400.1

Sequence Viewer

Length: 885 bp
ATGAGTATTCAAGGTTTAGTAGTACTTCTTTTTCTGTCTGTTTTGAGTGTGAGTACTATTGGGAGCACTCTTGTTGAAAATGGTTCTACGAGTACCACTCATCATAAGTCCAAGGGAAAAAGATGGGCACTTCTAATTGCTGGTTCAAATGGTTACGATAACTATCGACACCAGGCTGATGTATGCCATGCATACCAGATACTTAAGAAAGGTGGACTCAAAGATGAAAACATTATTGTCTTCATGTATGATGATATTGCTAACAACACAGAAAACCCTAGACCTGGTGTCATTATCAACAAGCCAAACGGCAAGGATGTCTACCACGGAGTTCCCAAGGTCCGAAGCAGAACAAATGAATCTCATGTCATGCAGTATGGAGATATGAGTCACATAAAGGAATTCCTCTACACTTACATGGGGGCAACTCCTAAAACTCATAGCTATGCTCCCTTCACATCCTCGCCTTCGGAATCAATCTCAAGGACTGTTAGCCAGCGGGATGTAGCCCTCCTATATTTTCTGCAAAAGTTAAACAGAGCTCCAACTGGCTCTCATGAGAAGATCGAAGCTCAAAAGCAGCTACGTAAAGAAATTGCTCGAAGGAAACATGTGGACTACAGCATAACCAAAATAGGGGAGCTTCTGTTTGGGACCGAGAGGAGCTCAAAAGTATTGATGAATGTCAGGCCTTCGGGACAACCTCTAGTGGATGACTGGGAGTGCTTCAAGATGTTTATAAGGACTTATGAGAGTCGCTGTGGAAAGCTATCGAAATATGGAATGAAATACACGCGAGCTTTTGCCAACATGTGCAATGCCGGGGTCAGCCTAGAGAAACTTGTTGCAGTGTCTGATCAAGCTTGTTCTAAGAAACATGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

294

Amino Acids

33.06

Weight (kDa)

9.54

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 41 - 113 2e-34 Peptidase C13 family
Legum_prodom PF20985 193 - 289 9.4e-40 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 740
AccI GTMKAC 1 cut(s) 321
AccII CGCG 1 cut(s) 796
AciI CCGC 1 cut(s) 499
AcsI RAATTY 1 cut(s) 401
AfaI GTAC 3 cut(s) 24, 55, 94
AfiI CCNNNNNNNGG 2 cut(s) 284, 636
AflII CTTAAG 1 cut(s) 203
AflIII ACRYGT 3 cut(s) 610, 810, 877
AgsI TTSAA 4 cut(s) 11, 77, 147, 730
AhdI GACNNNNNGTC 1 cut(s) 287
AjnI CCWGG 2 cut(s) 171, 283
AluBI AGCT 9 cut(s) 444, 542, 572, 583, 643, 666, 769, 800, 863
AluI AGCT 9 cut(s) 444, 542, 572, 583, 643, 666, 769, 800, 863
Alw21I GWGCWC 3 cut(s) 68, 544, 668
AlwNI CAGNNNCTG 1 cut(s) 854
AoxI GGCC 1 cut(s) 689
ApeKI GCWGC 1 cut(s) 580
ApoI RAATTY 1 cut(s) 401
AspS9I GGNCC 2 cut(s) 340, 654
AsuC2I CCSGG 1 cut(s) 823
AvaII GGWCC 2 cut(s) 340, 654
BaeGI GKGCMC 1 cut(s) 130
BanII GRGCYC 2 cut(s) 544, 668
BbsI GAAGAC 1 cut(s) 232
Bbv12I GWGCWC 3 cut(s) 68, 544, 668
BbvI GCAGC 1 cut(s) 592
BccI CCATC 1 cut(s) 117
BceAI ACGGC 1 cut(s) 325
BciT130I CCWGG 2 cut(s) 173, 285
BclI TGATCA 1 cut(s) 856
BcnI CCSGG 1 cut(s) 823
BfaI CTAG 3 cut(s) 279, 707, 833
BfmI CTRYAG 1 cut(s) 619
BfrI CTTAAG 1 cut(s) 203
BisI GCNGC 1 cut(s) 581
BlsI GCNGC 1 cut(s) 582
BmcAI AGTACT 2 cut(s) 24, 55
Bme1390I CCNGG 3 cut(s) 173, 285, 823
Bme18I GGWCC 2 cut(s) 340, 654
BmeRI GACNNNNNGTC 1 cut(s) 287
BmgT120I GGNCC 2 cut(s) 340, 654
BmiI GGNNCC 1 cut(s) 655
BmrFI CCNGG 3 cut(s) 173, 285, 823
BmrI ACTGGG 1 cut(s) 727
BmuI ACTGGG 1 cut(s) 727
BpiI GAAGAC 1 cut(s) 232
BplI GAGNNNNNCTC 4 cut(s) 82, 114, 650, 682
BpuEI CTTGAG 1 cut(s) 466
BpuMI CCSGG 1 cut(s) 823
BsaAI YACGTR 1 cut(s) 587
BsaBI GATNNNNATC 1 cut(s) 162
BsaJI CCNNGG 4 cut(s) 111, 325, 336, 822
BsaXI ACNNNNNCTCC 2 cut(s) 632, 662
Bsc4I CCNNNNNNNGG 2 cut(s) 284, 636
Bse1I ACTGG 2 cut(s) 553, 722
Bse3DI GCAATG 1 cut(s) 823
Bse8I GATNNNNATC 1 cut(s) 162
BseBI CCWGG 2 cut(s) 173, 285
BseDI CCNNGG 4 cut(s) 111, 325, 336, 822
BseGI GGATG 4 cut(s) 322, 458, 508, 718
BseJI GATNNNNATC 1 cut(s) 162
BseLI CCNNNNNNNGG 2 cut(s) 284, 636
BseMI GCAATG 1 cut(s) 823
BseNI ACTGG 2 cut(s) 553, 722
BseRI GAGGAG 1 cut(s) 676
BseSI GKGCMC 1 cut(s) 130
BseXI GCAGC 1 cut(s) 592
Bsh1236I CGCG 1 cut(s) 796
BshFI GGCC 1 cut(s) 691
BsiHKAI GWGCWC 3 cut(s) 68, 544, 668
BsiSI CCGG 1 cut(s) 822
BslFI GGGAC 2 cut(s) 667, 711
BslI CCNNNNNNNGG 2 cut(s) 284, 636
BsmFI GGGAC 2 cut(s) 667, 711
BsnI GGCC 1 cut(s) 691
Bsp1286I GDGCHC 4 cut(s) 68, 130, 544, 668
Bsp143I GATC 2 cut(s) 564, 856
BspACI CCGC 1 cut(s) 499
BspANI GGCC 1 cut(s) 691
BspFNI CGCG 1 cut(s) 796
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 1 cut(s) 655
BspTI CTTAAG 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 823
BsrI ACTGG 2 cut(s) 553, 722
BssECI CCNNGG 4 cut(s) 111, 325, 336, 822
BssMI GATC 2 cut(s) 564, 856
BssT1I CCWWGG 2 cut(s) 111, 336
Bst2UI CCWGG 2 cut(s) 173, 285
Bst4CI ACNGT 1 cut(s) 490
BstAFI CTTAAG 1 cut(s) 203
BstBAI YACGTR 1 cut(s) 587
BstC8I GCNNGC 2 cut(s) 497, 798
BstDEI CTNAG 1 cut(s) 870
BstDSI CCRYGG 1 cut(s) 325
BstF5I GGATG 4 cut(s) 322, 458, 508, 718
BstFNI CGCG 1 cut(s) 796
BstKTI GATC 2 cut(s) 567, 859
BstMBI GATC 2 cut(s) 564, 856
BstNI CCWGG 2 cut(s) 173, 285
BstNSI RCATGY 3 cut(s) 614, 814, 881
BstSCI CCNGG 3 cut(s) 171, 283, 821
BstSFI CTRYAG 1 cut(s) 619
BstSLI GKGCMC 1 cut(s) 130
BstSNI TACGTA 1 cut(s) 587
BstUI CGCG 1 cut(s) 796
BstV1I GCAGC 1 cut(s) 592
BstV2I GAAGAC 1 cut(s) 232
BsuRI GGCC 1 cut(s) 691
BtgI CCRYGG 1 cut(s) 325
BtsCI GGATG 4 cut(s) 322, 458, 508, 718
BtsI GCAGTG 1 cut(s) 855
BtsIMutI CAGTG 1 cut(s) 855
Cac8I GCNNGC 2 cut(s) 497, 798
CaiI CAGNNNCTG 1 cut(s) 854
CciI TCATGA 1 cut(s) 556
Cfr13I GGNCC 2 cut(s) 340, 654
CsiI ACCWGGT 1 cut(s) 283
Csp6I GTAC 3 cut(s) 23, 54, 93
CviAII CATG 9 cut(s) 188, 244, 365, 370, 418, 557, 611, 811, 878
CviQI GTAC 3 cut(s) 23, 54, 93
DdeI CTNAG 1 cut(s) 870
DpnI GATC 2 cut(s) 566, 858
DpnII GATC 2 cut(s) 564, 856
DriI GACNNNNNGTC 1 cut(s) 287
Eam1105I GACNNNNNGTC 1 cut(s) 287
Ecl136II GAGCTC 2 cut(s) 542, 666
Eco105I TACGTA 1 cut(s) 587
Eco130I CCWWGG 2 cut(s) 111, 336
Eco147I AGGCCT 1 cut(s) 691
Eco24I GRGCYC 2 cut(s) 544, 668
Eco47I GGWCC 2 cut(s) 340, 654
Eco53kI GAGCTC 2 cut(s) 542, 666
EcoICRI GAGCTC 2 cut(s) 542, 666
EcoRI GAATTC 1 cut(s) 401
EcoRII CCWGG 2 cut(s) 171, 283
EcoT14I CCWWGG 2 cut(s) 111, 336
EcoT22I ATGCAT 1 cut(s) 193
EcoT38I GRGCYC 2 cut(s) 544, 668
ErhI CCWWGG 2 cut(s) 111, 336
FaeI CATG 9 cut(s) 191, 247, 368, 373, 421, 560, 614, 814, 881
FaqI GGGAC 2 cut(s) 667, 711
FatI CATG 9 cut(s) 187, 243, 364, 369, 417, 556, 610, 810, 877
FauI CCCGC 1 cut(s) 492
FbaI TGATCA 1 cut(s) 856
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 581
FokI GGATG 4 cut(s) 329, 445, 515, 725
FriOI GRGCYC 2 cut(s) 544, 668
Fsp4HI GCNGC 1 cut(s) 581
FspBI CTAG 3 cut(s) 279, 707, 833
GluI GCNGC 1 cut(s) 581
HaeIII GGCC 1 cut(s) 691
HapII CCGG 1 cut(s) 822
Hin1II CATG 9 cut(s) 191, 247, 368, 373, 421, 560, 614, 814, 881
HindIII AAGCTT 1 cut(s) 861
HinfI GANTC 5 cut(s) 216, 359, 388, 473, 754
HpaII CCGG 1 cut(s) 822
Hpy166II GTNNAC 3 cut(s) 215, 322, 616
Hpy188I TCNGA 3 cut(s) 344, 472, 856
Hpy188III TCNNGA 3 cut(s) 557, 696, 730
Hpy8I GTNNAC 3 cut(s) 215, 322, 616
HpyAV CCTTC 4 cut(s) 463, 477, 597, 702
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4IV ACGT 1 cut(s) 586
HpyCH4V TGCA 5 cut(s) 191, 373, 526, 816, 848
HpyF3I CTNAG 1 cut(s) 870
HpySE526I ACGT 1 cut(s) 586
Hsp92II CATG 9 cut(s) 191, 247, 368, 373, 421, 560, 614, 814, 881
Ksp22I TGATCA 1 cut(s) 856
Kzo9I GATC 2 cut(s) 564, 856
LmnI GCTCC 5 cut(s) 63, 454, 547, 640, 663
Lsp1109I GCAGC 1 cut(s) 592
MabI ACCWGGT 1 cut(s) 283
MaeI CTAG 3 cut(s) 279, 707, 833
MaeII ACGT 1 cut(s) 586
MaeIII GTNAC 2 cut(s) 152, 389
MalI GATC 2 cut(s) 566, 858
MboI GATC 2 cut(s) 564, 856
MboII GAAGA 2 cut(s) 232, 574
MhlI GDGCHC 4 cut(s) 68, 130, 544, 668
MluCI AATT 3 cut(s) 135, 401, 594
MlyI GAGTC 3 cut(s) 210, 397, 763
MmeI TCCRAC 1 cut(s) 569
MnlI CCTC 5 cut(s) 416, 472, 521, 654, 714
Mph1103I ATGCAT 1 cut(s) 193
MseI TTAA 2 cut(s) 204, 533
MslI CAYNNNNRTG 2 cut(s) 416, 444
MspA1I CMGCKG 1 cut(s) 499
MspCI CTTAAG 1 cut(s) 203
MspI CCGG 1 cut(s) 822
MspR9I CCNGG 3 cut(s) 173, 285, 823
MvaI CCWGG 2 cut(s) 173, 285
MvnI CGCG 1 cut(s) 796
NciI CCSGG 1 cut(s) 823
NdeII GATC 2 cut(s) 564, 856
NlaIII CATG 9 cut(s) 191, 247, 368, 373, 421, 560, 614, 814, 881
NlaIV GGNNCC 1 cut(s) 655
NmuCI GTSAC 1 cut(s) 389
NsiI ATGCAT 1 cut(s) 193
NspI RCATGY 3 cut(s) 614, 814, 881
PagI TCATGA 1 cut(s) 556
PceI AGGCCT 1 cut(s) 691
PciI ACATGT 3 cut(s) 610, 810, 877
PfeI GAWTC 2 cut(s) 359, 473
PkrI GCNGC 1 cut(s) 582
PleI GAGTC 3 cut(s) 210, 396, 762
PpsI GAGTC 3 cut(s) 210, 396, 762
Ppu21I YACGTR 1 cut(s) 587
PscI ACATGT 3 cut(s) 610, 810, 877
PsiI TTATAA 1 cut(s) 740
Psp124BI GAGCTC 2 cut(s) 544, 668
Psp6I CCWGG 2 cut(s) 171, 283
PspGI CCWGG 2 cut(s) 171, 283
PspN4I GGNNCC 1 cut(s) 655
PspPI GGNCC 2 cut(s) 340, 654
PstNI CAGNNNCTG 1 cut(s) 854
RsaI GTAC 3 cut(s) 24, 55, 94
RsaNI GTAC 3 cut(s) 23, 54, 93
RseI CAYNNNNRTG 2 cut(s) 416, 444
SacI GAGCTC 2 cut(s) 544, 668
SaqAI TTAA 2 cut(s) 204, 533
SatI GCNGC 1 cut(s) 581
Sau3AI GATC 2 cut(s) 564, 856
Sau96I GGNCC 2 cut(s) 340, 654
ScaI AGTACT 2 cut(s) 24, 55
SchI GAGTC 3 cut(s) 210, 397, 763
ScrFI CCNGG 3 cut(s) 173, 285, 823
SduI GDGCHC 4 cut(s) 68, 130, 544, 668
SexAI ACCWGGT 1 cut(s) 283
SfcI CTRYAG 1 cut(s) 619
SinI GGWCC 2 cut(s) 340, 654
SmiMI CAYNNNNRTG 2 cut(s) 416, 444
SmlI CTYRAG 2 cut(s) 203, 481
SmoI CTYRAG 2 cut(s) 203, 481
SnaBI TACGTA 1 cut(s) 587
Sse9I AATT 3 cut(s) 135, 401, 594
SseBI AGGCCT 1 cut(s) 691
SsiI CCGC 1 cut(s) 499
SspMI CTAG 3 cut(s) 279, 707, 833
SstI GAGCTC 2 cut(s) 544, 668
StuI AGGCCT 1 cut(s) 691
StyD4I CCNGG 3 cut(s) 171, 283, 821
StyI CCWWGG 2 cut(s) 111, 336
TaaI ACNGT 1 cut(s) 490
TaiI ACGT 1 cut(s) 589
TaqI TCGA 4 cut(s) 166, 567, 601, 773
TaqII GACCGA 1 cut(s) 671
TasI AATT 3 cut(s) 135, 401, 594
TatI WGTACW 2 cut(s) 22, 53
TfiI GAWTC 2 cut(s) 359, 473
Tru1I TTAA 2 cut(s) 204, 533
Tru9I TTAA 2 cut(s) 204, 533
TscAI CASTG 1 cut(s) 855
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 1 cut(s) 580
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 5 cut(s) 232, 240, 372, 695, 800
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 1 cut(s) 855
Vha464I CTTAAG 1 cut(s) 203
VpaK11BI GGWCC 2 cut(s) 340, 654
XapI RAATTY 1 cut(s) 401
XceI RCATGY 3 cut(s) 614, 814, 881
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 3 cut(s) 279, 707, 833
ZrmI AGTACT 2 cut(s) 24, 55
Zsp2I ATGCAT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.