FvH4_6g37530

Protein trichome birefringence-like 43

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000737
Physical Location & Seq
Forward (+)
29558379 .. 29560776
2398 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g37530.t1

Sequence Viewer

Length: 1074 bp
ATGGGTACATTGGGATCTATTGCCATTGCAGTTTTGGTGCTAGTACTGCAACATGATCGAGTACATGGGAAGCATGCATATGAGAAGTTGAACAAGTGTGATATTTACCAAGGAAGCTGGGTTTACGACGATTCTTATCCTCTTTATGATTCAAGTCTCTGCAACTTCATAGAGAATGCGTTCAATTGCCAAAAAAATGGTCGACCCGACAAACACTATCTCAAATACAGATGGCAGCCCTCTTCATGCAAGTTACCAAGATTTGATGTCGAAGATTTATTGGAAAGATTGAGAAACAAGCGCATCATGTTTGTTGGAGACTCGCTAAGTTTGAACCAGTGGCAATCTCTCACATGTATGGTTCATGCAGCCTCACCACAATCTGAATACACTATTGAAAGAACAGGAAGCATCTCTACAATGAAATTCCTGAGTCACAATGCTTCTTTGATGCTTCAACGCAATGCGTTTCTTGTTGACATTGTAGAAGAAAGACAGGGGTGGGTTTTGAAGCTAAACTCCATTGATCATAATGATCAGAAAGCATGGAAAGACTCTGATGTATTGATTTTCAACTCATGGCATTGGTGGCTTCACACAGGAAGGAAACAACCGTGGAATTTTATTCAAGATGGAGATAATTTACATGAAGACATGGATCGCCTTGTTGCTTATGAGAAAGCCTTGAAAACATGGGCTCGATGGGTAGACAAAAATGTCGATCCTACCAAAACCAAAGTGTTCTACCAAAGCTTTTCACCTACTCATTGGAATTCAACTGGTTGGGGGTACCCTGAAGGGAAGAATTGCAGCAGCGAAACACAACCACTAATAGCGCCACCGTATCCAAGAAGCCGGCACCCGGCAGAACTGATTGTGGAAAGAGTATTGAGCAACATGTCAAAGCCGGTCGGTTTGCTCAATGTAACAGCATTGTCACGGCTACGGAAAGATGCACACCCATCCATATATGGCATCGGAGGGCACCACGGCATGGACTGCAGCCACTGGTGTCTAGCTGGAGTTCCTGATACTTGGAACAATCTTTTATACGCTGAGCTCATTCGAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

358

Amino Acids

41.3

Weight (kDa)

8.06

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 32 - 84 1.3e-23 PMR5 N terminal Domain
PC-Esterase PF13839 85 - 352 2.2e-92 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 716
Acc65I GGTACC 1 cut(s) 789
AccB1I GGYRCC 3 cut(s) 789, 858, 984
AccB7I CCANNNNNTGG 1 cut(s) 994
AccI GTMKAC 2 cut(s) 202, 708
AclWI GGATC 3 cut(s) 22, 666, 716
AcsI RAATTY 4 cut(s) 425, 619, 772, 1068
AcuI CTGAAG 1 cut(s) 816
AfaI GTAC 4 cut(s) 7, 45, 63, 791
AfiI CCNNNNNNNGG 2 cut(s) 862, 994
AflIII ACRYGT 2 cut(s) 353, 897
AluBI AGCT 5 cut(s) 117, 514, 753, 1019, 1060
AluI AGCT 5 cut(s) 117, 514, 753, 1019, 1060
Alw21I GWGCWC 1 cut(s) 1062
Alw26I GTCTC 2 cut(s) 161, 312
AlwI GGATC 3 cut(s) 22, 666, 716
AlwNI CAGNNNCTG 1 cut(s) 1008
ApeKI GCWGC 5 cut(s) 235, 368, 810, 813, 1002
ApoI RAATTY 4 cut(s) 425, 619, 772, 1068
ArsI GACNNNNNNTTYG 2 cut(s) 184, 216
Asp700I GAANNNNTTC 1 cut(s) 179
Asp718I GGTACC 1 cut(s) 789
AspLEI GCGC 2 cut(s) 303, 838
AsuC2I CCSGG 1 cut(s) 863
AsuHPI GGTGA 2 cut(s) 366, 750
AsuII TTCGAA 1 cut(s) 1066
BaeGI GKGCMC 1 cut(s) 987
BanI GGYRCC 3 cut(s) 789, 858, 984
BanII GRGCYC 2 cut(s) 700, 1062
BarI GAAGNNNNNNTAC 2 cut(s) 400, 432
BbsI GAAGAC 1 cut(s) 657
Bbv12I GWGCWC 1 cut(s) 1062
BbvI GCAGC 5 cut(s) 247, 380, 822, 825, 1014
BccI CCATC 4 cut(s) 225, 626, 696, 970
BceAI ACGGC 2 cut(s) 956, 1006
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciVI GTATCC 1 cut(s) 855
BclI TGATCA 2 cut(s) 526, 535
BcnI CCSGG 1 cut(s) 863
BcoDI GTCTC 2 cut(s) 161, 312
BfaI CTAG 2 cut(s) 41, 1016
BfmI CTRYAG 1 cut(s) 1000
BfoI RGCGCY 1 cut(s) 839
BfuI GTATCC 1 cut(s) 855
BisI GCNGC 5 cut(s) 236, 369, 811, 814, 1003
BlpI GCTNAGC 1 cut(s) 1056
BlsI GCNGC 5 cut(s) 237, 370, 812, 815, 1004
BmcAI AGTACT 1 cut(s) 45
Bme1390I CCNGG 1 cut(s) 863
BmiI GGNNCC 3 cut(s) 791, 860, 986
BmrFI CCNGG 1 cut(s) 863
BmsI GCATC 5 cut(s) 312, 420, 441, 943, 984
BpiI GAAGAC 1 cut(s) 657
BpmI CTGGAG 1 cut(s) 1041
Bpu1102I GCTNAGC 1 cut(s) 1056
Bpu14I TTCGAA 1 cut(s) 1066
BpuMI CCSGG 1 cut(s) 863
BsaBI GATNNNNATC 1 cut(s) 135
BsaJI CCNNGG 3 cut(s) 109, 614, 988
Bsc4I CCNNNNNNNGG 2 cut(s) 862, 994
Bse118I RCCGGY 2 cut(s) 855, 907
Bse1I ACTGG 3 cut(s) 337, 784, 1013
Bse3DI GCAATG 2 cut(s) 24, 469
Bse8I GATNNNNATC 1 cut(s) 135
BseDI CCNNGG 3 cut(s) 109, 614, 988
BseGI GGATG 1 cut(s) 962
BseJI GATNNNNATC 1 cut(s) 135
BseLI CCNNNNNNNGG 2 cut(s) 862, 994
BseMI GCAATG 2 cut(s) 24, 469
BseMII CTCAG 2 cut(s) 422, 1047
BseNI ACTGG 3 cut(s) 337, 784, 1013
BseSI GKGCMC 1 cut(s) 987
BseXI GCAGC 5 cut(s) 247, 380, 822, 825, 1014
BseYI CCCAGC 1 cut(s) 117
Bsh1285I CGRYCG 1 cut(s) 912
BshNI GGYRCC 3 cut(s) 789, 858, 984
BsiEI CGRYCG 1 cut(s) 912
BsiHKAI GWGCWC 1 cut(s) 1062
BsiSI CCGG 3 cut(s) 856, 863, 908
BslI CCNNNNNNNGG 2 cut(s) 862, 994
BsmAI GTCTC 2 cut(s) 161, 312
BsmI GAATGC 1 cut(s) 181
Bsp119I TTCGAA 1 cut(s) 1066
Bsp1286I GDGCHC 3 cut(s) 700, 987, 1062
Bsp143I GATC 6 cut(s) 14, 55, 526, 535, 658, 721
Bsp1720I GCTNAGC 1 cut(s) 1056
BspCNI CTCAG 2 cut(s) 423, 1048
BspLI GGNNCC 3 cut(s) 791, 860, 986
BspMAI CTGCAG 1 cut(s) 1004
BspPI GGATC 3 cut(s) 22, 666, 716
BspT104I TTCGAA 1 cut(s) 1066
BspT107I GGYRCC 3 cut(s) 789, 858, 984
BsrDI GCAATG 2 cut(s) 24, 469
BsrFI RCCGGY 2 cut(s) 855, 907
BsrI ACTGG 3 cut(s) 337, 784, 1013
BssAI RCCGGY 2 cut(s) 855, 907
BssECI CCNNGG 3 cut(s) 109, 614, 988
BssMI GATC 6 cut(s) 14, 55, 526, 535, 658, 721
BssT1I CCWWGG 1 cut(s) 109
Bst4CI ACNGT 2 cut(s) 615, 843
Bst6I CTCTTC 1 cut(s) 247
BstAPI GCANNNNNTGC 1 cut(s) 999
BstBI TTCGAA 1 cut(s) 1066
BstC8I GCNNGC 2 cut(s) 75, 857
BstDEI CTNAG 3 cut(s) 326, 431, 1056
BstDSI CCRYGG 2 cut(s) 614, 988
BstF5I GGATG 1 cut(s) 962
BstH2I RGCGCY 1 cut(s) 839
BstHHI GCGC 2 cut(s) 303, 838
BstKTI GATC 6 cut(s) 17, 58, 529, 538, 661, 724
BstMAI GTCTC 2 cut(s) 161, 312
BstMBI GATC 6 cut(s) 14, 55, 526, 535, 658, 721
BstMCI CGRYCG 1 cut(s) 912
BstMWI GCNNNNNNNGC 3 cut(s) 46, 589, 999
BstNSI RCATGY 3 cut(s) 77, 357, 901
BstSCI CCNGG 1 cut(s) 861
BstSFI CTRYAG 1 cut(s) 1000
BstSLI GKGCMC 1 cut(s) 987
BstV1I GCAGC 5 cut(s) 247, 380, 822, 825, 1014
BstV2I GAAGAC 1 cut(s) 657
BstX2I RGATCY 1 cut(s) 14
BstXI CCANNNNNNTGG 1 cut(s) 197
BstYI RGATCY 1 cut(s) 14
BsuI GTATCC 1 cut(s) 855
BtgI CCRYGG 2 cut(s) 614, 988
BtsCI GGATG 1 cut(s) 962
BtsIMutI CAGTG 2 cut(s) 344, 1006
Cac8I GCNNGC 2 cut(s) 75, 857
CaiI CAGNNNCTG 1 cut(s) 1008
CfoI GCGC 2 cut(s) 303, 838
Cfr10I RCCGGY 2 cut(s) 855, 907
Csp6I GTAC 4 cut(s) 6, 44, 62, 790
CviQI GTAC 4 cut(s) 6, 44, 62, 790
DdeI CTNAG 3 cut(s) 326, 431, 1056
DpnI GATC 6 cut(s) 16, 57, 528, 537, 660, 723
DpnII GATC 6 cut(s) 14, 55, 526, 535, 658, 721
DrdI GACNNNNNNGTC 1 cut(s) 716
DseDI GACNNNNNNGTC 1 cut(s) 716
Eam1104I CTCTTC 1 cut(s) 247
EarI CTCTTC 1 cut(s) 247
Ecl136II GAGCTC 1 cut(s) 1060
Eco130I CCWWGG 1 cut(s) 109
Eco24I GRGCYC 2 cut(s) 700, 1062
Eco53kI GAGCTC 1 cut(s) 1060
Eco57I CTGAAG 1 cut(s) 816
EcoICRI GAGCTC 1 cut(s) 1060
EcoRI GAATTC 1 cut(s) 772
EcoT14I CCWWGG 1 cut(s) 109
EcoT22I ATGCAT 1 cut(s) 79
EcoT38I GRGCYC 2 cut(s) 700, 1062
ErhI CCWWGG 1 cut(s) 109
FauNDI CATATG 1 cut(s) 79
FbaI TGATCA 2 cut(s) 526, 535
FblI GTMKAC 2 cut(s) 202, 708
Fnu4HI GCNGC 5 cut(s) 236, 369, 811, 814, 1003
FokI GGATG 1 cut(s) 949
FriOI GRGCYC 2 cut(s) 700, 1062
Fsp4HI GCNGC 5 cut(s) 236, 369, 811, 814, 1003
FspBI CTAG 2 cut(s) 41, 1016
GlaI GCGC 2 cut(s) 302, 837
GluI GCNGC 5 cut(s) 236, 369, 811, 814, 1003
GsaI CCCAGC 1 cut(s) 121
GsuI CTGGAG 1 cut(s) 1041
HaeII RGCGCY 1 cut(s) 839
HapII CCGG 3 cut(s) 856, 863, 908
HhaI GCGC 2 cut(s) 303, 838
Hin6I GCGC 2 cut(s) 301, 836
HinP1I GCGC 2 cut(s) 301, 836
HincII GTYRAC 2 cut(s) 203, 478
HindII GTYRAC 2 cut(s) 203, 478
HindIII AAGCTT 1 cut(s) 751
HinfI GANTC 5 cut(s) 131, 149, 320, 433, 554
HpaII CCGG 3 cut(s) 856, 863, 908
HphI GGTGA 2 cut(s) 366, 750
Hpy166II GTNNAC 4 cut(s) 124, 203, 478, 709
Hpy188I TCNGA 4 cut(s) 385, 540, 559, 980
Hpy188III TCNNGA 3 cut(s) 430, 629, 1028
Hpy8I GTNNAC 4 cut(s) 124, 203, 478, 709
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 2 cut(s) 597, 791
HpyCH4III ACNGT 2 cut(s) 615, 843
HpyCH4V TGCA 9 cut(s) 29, 49, 77, 162, 249, 368, 810, 956, 1002
HpyF10VI GCNNNNNNNGC 3 cut(s) 46, 589, 999
HpyF3I CTNAG 3 cut(s) 326, 431, 1056
HspAI GCGC 2 cut(s) 301, 836
KpnI GGTACC 1 cut(s) 793
KroI GCCGGC 1 cut(s) 855
KroNI GCCGGC 1 cut(s) 857
Ksp22I TGATCA 2 cut(s) 526, 535
Kzo9I GATC 6 cut(s) 14, 55, 526, 535, 658, 721
Lsp1109I GCAGC 5 cut(s) 247, 380, 822, 825, 1014
LweI GCATC 5 cut(s) 312, 420, 441, 943, 984
MaeI CTAG 2 cut(s) 41, 1016
MaeIII GTNAC 4 cut(s) 252, 434, 925, 936
MalI GATC 6 cut(s) 16, 57, 528, 537, 660, 723
MboI GATC 6 cut(s) 14, 55, 526, 535, 658, 721
MboII GAAGA 5 cut(s) 234, 284, 500, 662, 814
MfeI CAATTG 1 cut(s) 184
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 3 cut(s) 700, 987, 1062
MluCI AATT 7 cut(s) 184, 425, 619, 640, 772, 805, 1068
MlyI GAGTC 3 cut(s) 314, 442, 548
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 4 cut(s) 150, 250, 382, 974
Mph1103I ATGCAT 1 cut(s) 79
MroNI GCCGGC 1 cut(s) 855
MroXI GAANNNNTTC 1 cut(s) 179
MslI CAYNNNNRTG 2 cut(s) 78, 356
MspI CCGG 3 cut(s) 856, 863, 908
MspR9I CCNGG 1 cut(s) 863
MunI CAATTG 1 cut(s) 184
Mva1269I GAATGC 1 cut(s) 181
MwoI GCNNNNNNNGC 3 cut(s) 46, 589, 999
NaeI GCCGGC 1 cut(s) 857
NciI CCSGG 1 cut(s) 863
NdeI CATATG 1 cut(s) 79
NdeII GATC 6 cut(s) 14, 55, 526, 535, 658, 721
NgoMIV GCCGGC 1 cut(s) 855
NlaIV GGNNCC 3 cut(s) 791, 860, 986
NmuCI GTSAC 2 cut(s) 434, 936
NsiI ATGCAT 1 cut(s) 79
NspI RCATGY 3 cut(s) 77, 357, 901
NspV TTCGAA 1 cut(s) 1066
PaeI GCATGC 1 cut(s) 77
PciI ACATGT 2 cut(s) 353, 897
PctI GAATGC 1 cut(s) 181
PdiI GCCGGC 1 cut(s) 857
PdmI GAANNNNTTC 1 cut(s) 179
PfeI GAWTC 2 cut(s) 131, 149
PflMI CCANNNNNTGG 1 cut(s) 994
PkrI GCNGC 5 cut(s) 237, 370, 812, 815, 1004
PleI GAGTC 3 cut(s) 314, 441, 548
PpsI GAGTC 3 cut(s) 314, 441, 548
PscI ACATGT 2 cut(s) 353, 897
Psp124BI GAGCTC 1 cut(s) 1062
PspFI CCCAGC 1 cut(s) 117
PspN4I GGNNCC 3 cut(s) 791, 860, 986
PstI CTGCAG 1 cut(s) 1004
PstNI CAGNNNCTG 1 cut(s) 1008
PsuI RGATCY 1 cut(s) 14
RsaI GTAC 4 cut(s) 7, 45, 63, 791
RsaNI GTAC 4 cut(s) 6, 44, 62, 790
RseI CAYNNNNRTG 2 cut(s) 78, 356
SacI GAGCTC 1 cut(s) 1062
SalI GTCGAC 1 cut(s) 201
SatI GCNGC 5 cut(s) 236, 369, 811, 814, 1003
Sau3AI GATC 6 cut(s) 14, 55, 526, 535, 658, 721
ScaI AGTACT 1 cut(s) 45
SchI GAGTC 3 cut(s) 314, 442, 548
ScrFI CCNGG 1 cut(s) 863
SduI GDGCHC 3 cut(s) 700, 987, 1062
SetI ASST 6 cut(s) 119, 516, 755, 763, 1021, 1062
SfaNI GCATC 5 cut(s) 312, 420, 441, 943, 984
SfcI CTRYAG 1 cut(s) 1000
SfuI TTCGAA 1 cut(s) 1066
SmiMI CAYNNNNRTG 2 cut(s) 78, 356
SphI GCATGC 1 cut(s) 77
Sse9I AATT 7 cut(s) 184, 425, 619, 640, 772, 805, 1068
SspMI CTAG 2 cut(s) 41, 1016
SstI GAGCTC 1 cut(s) 1062
StyD4I CCNGG 1 cut(s) 861
StyI CCWWGG 1 cut(s) 109
TaaI ACNGT 2 cut(s) 615, 843
TaqI TCGA 6 cut(s) 58, 202, 270, 700, 720, 1066
TasI AATT 7 cut(s) 184, 425, 619, 640, 772, 805, 1068
TatI WGTACW 2 cut(s) 43, 61
TfiI GAWTC 2 cut(s) 131, 149
TscAI CASTG 2 cut(s) 344, 1013
TseFI GTSAC 2 cut(s) 434, 936
TseI GCWGC 5 cut(s) 235, 368, 810, 813, 1002
Tsp45I GTSAC 2 cut(s) 434, 936
TspDTI ATGAA 5 cut(s) 157, 234, 353, 437, 663
TspGWI ACGGA 1 cut(s) 961
TspRI CASTG 2 cut(s) 344, 1013
Van91I CCANNNNNTGG 1 cut(s) 994
XapI RAATTY 4 cut(s) 425, 619, 772, 1068
XceI RCATGY 3 cut(s) 77, 357, 901
XcmI CCANNNNNNNNNTGG 1 cut(s) 31
XmiI GTMKAC 2 cut(s) 202, 708
XmnI GAANNNNTTC 1 cut(s) 179
XspI CTAG 2 cut(s) 41, 1016
ZrmI AGTACT 1 cut(s) 45
Zsp2I ATGCAT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.