Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000737

Physical Location & Seq

Genomic Coordinates Reverse (-)

27804030 .. 27807724

Gene Structure 3695 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1230000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1143 bp

ATGATATATGCATATATATGTCTTGTGAGTTGGACGACCAGCCTTCTAATATCCCTCTGTGTTTTCATGGCTTCTTTTGCAATTGGAGCTGCAGTATCACTGCTTGTATTTTCTCTTCTGCATCACCAAGTGCATGGAGGACTTGTAGGGAGCGTTATCAAAGGCTGCGATCTTTCTCACGGAAACTGGGTTTTTGATGATTCATATCCTCTTTATGCCGCGCCGACCTGCCCTTTCATTGAGACGGTATTTGATTGCGTAGGGAATGGCCGCCCGGACAAAAACTACCTCAAGTATAGATGGCAACCCTTTGCCTGCGGCTTACCAAGATTCGATGGAAGTACTTTCTTGTCGACGCATAGAGGGAGAAACATATTGTTTGTCGGGGACTCGTTGAGTTTGAACCAATGGCAGTCACTTACTTGTATGCTTTATACGGTTGTTCCAGGAACCAAATACACATTGGTCACGAAAGGAGGGCTCTCTACATTCACGTTCCTGGAATACAATCTGAAGGTGTCCTTCTCCCGCAATGCCTTCATTGTCGACATTGTAGGCACACCTGATGGCCTTGTCCTCAAACTCGACTCAATCTCACCCGAAAACGAGCAGCTGTGGCTGCAAAACGACGTATTGATCTTCGACTCGTGGCACTGGTGGCTTCACGTTGGAAGAAAGCAACCATGGGATTTGATTCAAGTGGGGAATCAAACTTACAAGGACATGGATCGCTTGGTTGCATATGAGAAGGCGTTGACCACTTGGGCTGGATGGGTTGACTCCAAAGTAGACCCCAACAAAACCAAGGTCTTCTTCCAAGGCGTTTCTCCAGATCATAGCAATGCAACAGAGTGGGGCGATCCTCAAGCAATTAACTGCAATGCACAAACAGAACCACTGCCAGGTCCAGACATTCCAGGAAAACCACATCCAGCAGAGCAAGTACTAGAGAAAGCGTTAAGTACCGCGTCAAAGCCGGTCCATTTGCTGAATATTACAGCTCTTTCGCAACTGCGAAAGGATGCGCACCCGTCGGTCTATGGCCTCGGCGGGCACAAGGGCATGGACTGCAGCCACTGGTGTTTAGCTGGAGTTCCTGATACTTGGAATGTGCTTCTTCATGCAGCTCTTACTCCAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008374 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071554

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

381

Amino Acids

42.2

Weight (kDa)

6.25

Isoelectric Point (pI)

30.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	55 - 106	8.5e-22	PMR5 N terminal Domain
PC-Esterase	PF13839	108 - 375	3.5e-87	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000737)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g18101 FvH4_4g19380 FvH4_5g32790 FvH4_5g32790 FvH4_6g27901 FvH4_6g37530
malus_domestica	MD09G1233400.v1.1 MD09G1233600.v1.1 MD17G1143400.v1.1 MD17G1229900.v1.1 MD17G1230000.v1.1
prunus_persica	Prupe.3G108000_v2.0.a1 Prupe.3G108000_v2.0.a1 Prupe.3G108200_v2.0.a1 Prupe.3G108300_v2.0.a1 Prupe.3G108300_v2.0.a1 Prupe.3G108300_v2.0.a1 Prupe.3G108300_v2.0.a1 Prupe.3G168700_v2.0.a1
pyrus_communis	pycom09g15180 pycom17g23260 pycom17g23270
rosa_chinensis	RchiOBHm_Chr2g0131401 RchiOBHm_Chr2g0131411 RchiOBHm_Chr2g0131441 RchiOBHm_Chr2g0150421 RchiOBHm_Chr4g0424421
rosa_laevigata	RLG00000007476 RLG00000019182 RLG00000019183 RLG00000020497
rosa_multiflora	Rmu_sc0003445.1_g000007 Rmu_sc0003567.1_g000013 Rmu_sc0005652.1_g000008 Rmu_sc0005652.1_g000023 Rmu_sc0005652.1_g000030 Rmu_sc0016958.1_g000002 Rmu_sc0043021.1_g000001 Rmu_ssc0000480.1_g000015
rosa_roxburghii	Rroxscaffold_2G00097140 Rroxscaffold_2G00112870 Rroxscaffold_2G00112970 Rroxscaffold_2G00112980 Rroxscaffold_5G00366360
rosa_rugosa	Rorug02G0296600 Rorug02G0296700 Rorug02G0421100 Rorug02G0421200 Rorug04G0197700
rosa_samantha	Rh2AG350000 Rh2AG350400 Rh2AG480500 Rh2BG356700 Rh2BG356800 Rh2BG492600 Rh2CG335500 Rh2CG335600 Rh2CG335700 Rh2CG335900 Rh2CG466800 Rh2DG374400 Rh2DG501400 Rh4AG253500 Rh4BG258900 Rh4CG270200 Rh4DG253500
rosa_wichuraiana	Rw2G028380 Rw2G028390 Rw2G028420 Rw2G039220 Rw4G021880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1026
Acc36I	ACCTGC	1 cut(s)	236
AccI	GTMKAC	3 cut(s)	353, 546, 789
AccII	CGCG	2 cut(s)	221, 968
AciI	CCGC	6 cut(s)	219, 271, 318, 529, 966, 1050
AclWI	GGATC	2 cut(s)	735, 854
AcoI	YGGCCR	1 cut(s)	268
AcuI	CTGAAG	1 cut(s)	533
AdeI	CACNNNGTG	1 cut(s)	130
AfaI	GTAC	3 cut(s)	343, 945, 964
AfiI	CCNNNNNNNGG	1 cut(s)	902
AgsI	TTSAA	2 cut(s)	403, 698
AjnI	CCWGG	4 cut(s)	445, 498, 901, 916
AjuI	GAANNNNNNNTTGG	2 cut(s)	797, 829
AluBI	AGCT	5 cut(s)	89, 613, 1001, 1088, 1127
AluI	AGCT	5 cut(s)	89, 613, 1001, 1088, 1127
Alw26I	GTCTC	1 cut(s)	236
AlwI	GGATC	2 cut(s)	735, 854
AlwNI	CAGNNNCTG	1 cut(s)	1077
AoxI	GGCC	3 cut(s)	268, 568, 1042
ApeKI	GCWGC	6 cut(s)	89, 165, 610, 619, 1071, 1124
ArsI	GACNNNNNNTTYG	2 cut(s)	792, 824
AspLEI	GCGC	2 cut(s)	223, 1027
AspS9I	GGNCC	2 cut(s)	905, 979
AsuC2I	CCSGG	1 cut(s)	275
AsuHPI	GGTGA	2 cut(s)	116, 588
AvaII	GGWCC	2 cut(s)	905, 979
BaeGI	GKGCMC	1 cut(s)	1056
BanII	GRGCYC	1 cut(s)	483
BarI	GAAGNNNNNNTAC	2 cut(s)	99, 131
BauI	CACGAG	1 cut(s)	646
BbsI	GAAGAC	1 cut(s)	802
BbvI	GCAGC	6 cut(s)	76, 152, 606, 622, 1083, 1136
BccI	CCATC	4 cut(s)	294, 329, 560, 765
BciT130I	CCWGG	4 cut(s)	447, 500, 903, 918
BcnI	CCSGG	1 cut(s)	275
BcoDI	GTCTC	1 cut(s)	236
BfaI	CTAG	1 cut(s)	947
BfmI	CTRYAG	2 cut(s)	90, 1069
BfuAI	ACCTGC	1 cut(s)	236
BisI	GCNGC	9 cut(s)	90, 166, 219, 271, 319, 611, 620, 1072, 1125
BlsI	GCNGC	9 cut(s)	91, 167, 220, 272, 320, 612, 621, 1073, 1126
BmcAI	AGTACT	2 cut(s)	343, 945
Bme1390I	CCNGG	5 cut(s)	275, 447, 500, 903, 918
Bme18I	GGWCC	2 cut(s)	905, 979
BmgT120I	GGNCC	2 cut(s)	905, 979
BmiI	GGNNCC	1 cut(s)	451
BmrFI	CCNGG	5 cut(s)	275, 447, 500, 903, 918
BmrI	ACTGGG	1 cut(s)	196
BmsI	GCATC	2 cut(s)	130, 1012
BmuI	ACTGGG	1 cut(s)	196
BpiI	GAAGAC	1 cut(s)	802
BpmI	CTGGAG	2 cut(s)	813, 1110
BpuEI	CTTGAG	2 cut(s)	275, 849
BpuMI	CCSGG	1 cut(s)	275
BsaBI	GATNNNNATC	1 cut(s)	204
BsaJI	CCNNGG	4 cut(s)	683, 804, 817, 1045
BsaXI	ACNNNNNCTCC	4 cut(s)	78, 108, 129, 159
Bsc4I	CCNNNNNNNGG	1 cut(s)	902
Bse118I	RCCGGY	1 cut(s)	976
Bse1I	ACTGG	3 cut(s)	191, 659, 1082
Bse3DI	GCAATG	3 cut(s)	538, 847, 886
Bse8I	GATNNNNATC	1 cut(s)	204
BseBI	CCWGG	4 cut(s)	447, 500, 903, 918
BseDI	CCNNGG	4 cut(s)	683, 804, 817, 1045
BseGI	GGATG	3 cut(s)	776, 928, 1027
BseJI	GATNNNNATC	1 cut(s)	204
BseLI	CCNNNNNNNGG	1 cut(s)	902
BseMI	GCAATG	3 cut(s)	538, 847, 886
BseNI	ACTGG	3 cut(s)	191, 659, 1082
BseSI	GKGCMC	1 cut(s)	1056
BseXI	GCAGC	6 cut(s)	76, 152, 606, 622, 1083, 1136
Bsh1236I	CGCG	2 cut(s)	221, 968
BshFI	GGCC	3 cut(s)	270, 570, 1044
BsiSI	CCGG	2 cut(s)	275, 977
BslFI	GGGAC	1 cut(s)	401
BslI	CCNNNNNNNGG	1 cut(s)	902
BsmAI	GTCTC	1 cut(s)	236
BsmBI	CGTCTC	1 cut(s)	236
BsmFI	GGGAC	1 cut(s)	401
BsnI	GGCC	3 cut(s)	270, 570, 1044
Bsp1286I	GDGCHC	2 cut(s)	483, 1056
Bsp143I	GATC	5 cut(s)	169, 636, 727, 832, 859
Bsp19I	CCATGG	1 cut(s)	683
BspACI	CCGC	6 cut(s)	219, 271, 318, 529, 966, 1050
BspANI	GGCC	3 cut(s)	270, 570, 1044
BspFNI	CGCG	2 cut(s)	221, 968
BspLI	GGNNCC	1 cut(s)	451
BspMAI	CTGCAG	2 cut(s)	94, 1073
BspMI	ACCTGC	1 cut(s)	236
BspPI	GGATC	2 cut(s)	735, 854
BsrDI	GCAATG	3 cut(s)	538, 847, 886
BsrFI	RCCGGY	1 cut(s)	976
BsrI	ACTGG	3 cut(s)	191, 659, 1082
BssAI	RCCGGY	1 cut(s)	976
BssECI	CCNNGG	4 cut(s)	683, 804, 817, 1045
BssMI	GATC	5 cut(s)	169, 636, 727, 832, 859
BssSI	CACGAG	1 cut(s)	646
BssT1I	CCWWGG	3 cut(s)	683, 804, 817
Bst2BI	CACGAG	1 cut(s)	646
Bst2UI	CCWGG	4 cut(s)	447, 500, 903, 918
Bst4CI	ACNGT	2 cut(s)	247, 439
Bst6I	CTCTTC	1 cut(s)	120
BstAPI	GCANNNNNTGC	1 cut(s)	1068
BstC8I	GCNNGC	2 cut(s)	316, 1052
BstDSI	CCRYGG	1 cut(s)	683
BstF5I	GGATG	3 cut(s)	776, 928, 1027
BstFNI	CGCG	2 cut(s)	221, 968
BstHHI	GCGC	2 cut(s)	223, 1027
BstKTI	GATC	5 cut(s)	172, 639, 730, 835, 862
BstMAI	GTCTC	1 cut(s)	236
BstMBI	GATC	5 cut(s)	169, 636, 727, 832, 859
BstMWI	GCNNNNNNNGC	6 cut(s)	77, 86, 616, 619, 658, 1068
BstNI	CCWGG	4 cut(s)	447, 500, 903, 918
BstSCI	CCNGG	5 cut(s)	273, 445, 498, 901, 916
BstSFI	CTRYAG	2 cut(s)	90, 1069
BstSLI	GKGCMC	1 cut(s)	1056
BstUI	CGCG	2 cut(s)	221, 968
BstV1I	GCAGC	6 cut(s)	76, 152, 606, 622, 1083, 1136
BstV2I	GAAGAC	1 cut(s)	802
BstXI	CCANNNNNNTGG	1 cut(s)	134
BsuRI	GGCC	3 cut(s)	270, 570, 1044
BtgI	CCRYGG	1 cut(s)	683
BtsCI	GGATG	3 cut(s)	776, 928, 1027
BtsI	GCAGTG	2 cut(s)	98, 896
BtsIMutI	CAGTG	4 cut(s)	98, 652, 896, 1075
BveI	ACCTGC	1 cut(s)	236
Cac8I	GCNNGC	2 cut(s)	316, 1052
CaiI	CAGNNNCTG	1 cut(s)	1077
CfoI	GCGC	2 cut(s)	223, 1027
Cfr10I	RCCGGY	1 cut(s)	976
Cfr13I	GGNCC	2 cut(s)	905, 979
CseI	GACGC	2 cut(s)	364, 957
Csp6I	GTAC	3 cut(s)	342, 944, 963
CviAII	CATG	6 cut(s)	67, 134, 684, 724, 1063, 1121
CviQI	GTAC	3 cut(s)	342, 944, 963
DpnI	GATC	5 cut(s)	171, 638, 729, 834, 861
DpnII	GATC	5 cut(s)	169, 636, 727, 832, 859
DraIII	CACNNNGTG	1 cut(s)	130
EaeI	YGGCCR	1 cut(s)	268
Eam1104I	CTCTTC	1 cut(s)	120
EarI	CTCTTC	1 cut(s)	120
Eco130I	CCWWGG	3 cut(s)	683, 804, 817
Eco24I	GRGCYC	1 cut(s)	483
Eco47I	GGWCC	2 cut(s)	905, 979
Eco57I	CTGAAG	1 cut(s)	533
EcoRII	CCWGG	4 cut(s)	445, 498, 901, 916
EcoT14I	CCWWGG	3 cut(s)	683, 804, 817
EcoT22I	ATGCAT	1 cut(s)	13
EcoT38I	GRGCYC	1 cut(s)	483
ErhI	CCWWGG	3 cut(s)	683, 804, 817
Esp3I	CGTCTC	1 cut(s)	236
FaeI	CATG	6 cut(s)	70, 137, 687, 727, 1066, 1124
FalI	AAGNNNNNCTT	4 cut(s)	506, 538, 797, 829
FaqI	GGGAC	1 cut(s)	401
FatI	CATG	6 cut(s)	66, 133, 683, 723, 1062, 1120
FauI	CCCGC	2 cut(s)	536, 1043
FauNDI	CATATG	1 cut(s)	742
FblI	GTMKAC	3 cut(s)	353, 546, 789
Fnu4HI	GCNGC	9 cut(s)	90, 166, 219, 271, 319, 611, 620, 1072, 1125
FokI	GGATG	3 cut(s)	783, 915, 1034
FriOI	GRGCYC	1 cut(s)	483
Fsp4HI	GCNGC	9 cut(s)	90, 166, 219, 271, 319, 611, 620, 1072, 1125
FspAI	RTGCGCAY	1 cut(s)	1026
FspBI	CTAG	1 cut(s)	947
FspI	TGCGCA	1 cut(s)	1026
GlaI	GCGC	2 cut(s)	222, 1026
GluI	GCNGC	9 cut(s)	90, 166, 219, 271, 319, 611, 620, 1072, 1125
GsuI	CTGGAG	2 cut(s)	813, 1110
HaeIII	GGCC	3 cut(s)	270, 570, 1044
HapII	CCGG	2 cut(s)	275, 977
HgaI	GACGC	2 cut(s)	364, 957
HhaI	GCGC	2 cut(s)	223, 1027
Hin1II	CATG	6 cut(s)	70, 137, 687, 727, 1066, 1124
Hin6I	GCGC	2 cut(s)	221, 1025
HinP1I	GCGC	2 cut(s)	221, 1025
HincII	GTYRAC	4 cut(s)	354, 547, 756, 778
HindII	GTYRAC	4 cut(s)	354, 547, 756, 778
HinfI	GANTC	8 cut(s)	200, 330, 389, 587, 644, 694, 706, 779
HpaII	CCGG	2 cut(s)	275, 977
HphI	GGTGA	2 cut(s)	116, 588
Hpy166II	GTNNAC	5 cut(s)	354, 547, 756, 778, 790
Hpy188I	TCNGA	1 cut(s)	513
Hpy188III	TCNNGA	4 cut(s)	469, 830, 908, 1097
Hpy8I	GTNNAC	5 cut(s)	354, 547, 756, 778, 790
Hpy99I	CGWCG	3 cut(s)	358, 632, 1036
HpyAV	CCTTC	5 cut(s)	53, 508, 532, 547, 742
HpyCH4III	ACNGT	2 cut(s)	247, 439
HpyCH4IV	ACGT	3 cut(s)	494, 630, 666
HpyF10VI	GCNNNNNNNGC	6 cut(s)	77, 86, 616, 619, 658, 1068
HpySE526I	ACGT	3 cut(s)	494, 630, 666
Hsp92II	CATG	6 cut(s)	70, 137, 687, 727, 1066, 1124
HspAI	GCGC	2 cut(s)	221, 1025
Kzo9I	GATC	5 cut(s)	169, 636, 727, 832, 859
LmnI	GCTCC	2 cut(s)	86, 150
Lsp1109I	GCAGC	6 cut(s)	76, 152, 606, 622, 1083, 1136
LweI	GCATC	2 cut(s)	130, 1012
MaeI	CTAG	1 cut(s)	947
MaeII	ACGT	3 cut(s)	494, 630, 666
MaeIII	GTNAC	2 cut(s)	414, 466
MalI	GATC	5 cut(s)	171, 638, 729, 834, 861
MboI	GATC	5 cut(s)	169, 636, 727, 832, 859
MboII	GAAGA	6 cut(s)	107, 631, 684, 802, 805, 1109
MfeI	CAATTG	1 cut(s)	81
MhlI	GDGCHC	2 cut(s)	483, 1056
MluCI	AATT	3 cut(s)	81, 870, 1138
MlyI	GAGTC	4 cut(s)	383, 581, 638, 773
MmeI	TCCRAC	2 cut(s)	11, 649
MnlI	CCTC	9 cut(s)	65, 131, 219, 299, 356, 470, 587, 873, 1055
Mph1103I	ATGCAT	1 cut(s)	13
MseI	TTAA	2 cut(s)	873, 959
MslI	CAYNNNNRTG	2 cut(s)	16, 840
MspA1I	CMGCKG	1 cut(s)	613
MspI	CCGG	2 cut(s)	275, 977
MspR9I	CCNGG	5 cut(s)	275, 447, 500, 903, 918
MunI	CAATTG	1 cut(s)	81
MvaI	CCWGG	4 cut(s)	447, 500, 903, 918
MvnI	CGCG	2 cut(s)	221, 968
MwoI	GCNNNNNNNGC	6 cut(s)	77, 86, 616, 619, 658, 1068
NciI	CCSGG	1 cut(s)	275
NcoI	CCATGG	1 cut(s)	683
NdeI	CATATG	1 cut(s)	742
NdeII	GATC	5 cut(s)	169, 636, 727, 832, 859
NlaIII	CATG	6 cut(s)	70, 137, 687, 727, 1066, 1124
NlaIV	GGNNCC	1 cut(s)	451
NmeAIII	GCCGAG	1 cut(s)	1026
NmuCI	GTSAC	2 cut(s)	414, 466
NsbI	TGCGCA	1 cut(s)	1026
NsiI	ATGCAT	1 cut(s)	13
PfeI	GAWTC	4 cut(s)	200, 330, 694, 706
PfoI	TCCNGGA	3 cut(s)	445, 498, 916
PkrI	GCNGC	9 cut(s)	91, 167, 220, 272, 320, 612, 621, 1073, 1126
PleI	GAGTC	4 cut(s)	383, 581, 638, 773
PpsI	GAGTC	4 cut(s)	383, 581, 638, 773
Psp6I	CCWGG	4 cut(s)	445, 498, 901, 916
PspGI	CCWGG	4 cut(s)	445, 498, 901, 916
PspN4I	GGNNCC	1 cut(s)	451
PspPI	GGNCC	2 cut(s)	905, 979
PstI	CTGCAG	2 cut(s)	94, 1073
PstNI	CAGNNNCTG	1 cut(s)	1077
PvuII	CAGCTG	1 cut(s)	613
RsaI	GTAC	3 cut(s)	343, 945, 964
RsaNI	GTAC	3 cut(s)	342, 944, 963
RseI	CAYNNNNRTG	2 cut(s)	16, 840
SalI	GTCGAC	2 cut(s)	352, 545
SaqAI	TTAA	2 cut(s)	873, 959
SatI	GCNGC	9 cut(s)	90, 166, 219, 271, 319, 611, 620, 1072, 1125
Sau3AI	GATC	5 cut(s)	169, 636, 727, 832, 859
Sau96I	GGNCC	2 cut(s)	905, 979
ScaI	AGTACT	2 cut(s)	343, 945
SchI	GAGTC	4 cut(s)	383, 581, 638, 773
ScrFI	CCNGG	5 cut(s)	275, 447, 500, 903, 918
SduI	GDGCHC	2 cut(s)	483, 1056
SfaNI	GCATC	2 cut(s)	130, 1012
SfcI	CTRYAG	2 cut(s)	90, 1069
SinI	GGWCC	2 cut(s)	905, 979
SmiMI	CAYNNNNRTG	2 cut(s)	16, 840
SmlI	CTYRAG	2 cut(s)	290, 864
SmoI	CTYRAG	2 cut(s)	290, 864
Sse9I	AATT	3 cut(s)	81, 870, 1138
SsiI	CCGC	6 cut(s)	219, 271, 318, 529, 966, 1050
SspI	AATATT	1 cut(s)	994
SspMI	CTAG	1 cut(s)	947
StyD4I	CCNGG	5 cut(s)	273, 445, 498, 901, 916
StyI	CCWWGG	3 cut(s)	683, 804, 817
TaaI	ACNGT	2 cut(s)	247, 439
TaiI	ACGT	3 cut(s)	497, 633, 669
TaqI	TCGA	5 cut(s)	333, 353, 546, 585, 642
TaqII	GACCGA	1 cut(s)	1024
TasI	AATT	3 cut(s)	81, 870, 1138
TatI	WGTACW	2 cut(s)	341, 943
TauI	GCSGC	3 cut(s)	221, 273, 321
TfiI	GAWTC	4 cut(s)	200, 330, 694, 706
Tru1I	TTAA	2 cut(s)	873, 959
Tru9I	TTAA	2 cut(s)	873, 959
TscAI	CASTG	4 cut(s)	105, 659, 903, 1082
TseFI	GTSAC	2 cut(s)	414, 466
TseI	GCWGC	6 cut(s)	89, 165, 610, 619, 1071, 1124
Tsp45I	GTSAC	2 cut(s)	414, 466
TspDTI	ATGAA	5 cut(s)	55, 192, 226, 529, 1109
TspGWI	ACGGA	1 cut(s)	195
TspRI	CASTG	4 cut(s)	105, 659, 903, 1082
VpaK11BI	GGWCC	2 cut(s)	905, 979
XcmI	CCANNNNNNNNNTGG	1 cut(s)	460
XmiI	GTMKAC	3 cut(s)	353, 546, 789
XspI	CTAG	1 cut(s)	947
ZrmI	AGTACT	2 cut(s)	343, 945
Zsp2I	ATGCAT	1 cut(s)	13

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.