RchiOBHm_Chr2g0150421

Protein trichome birefringence-like 43

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000737
Physical Location & Seq
Forward (+)
68083804 .. 68085870
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51976

Sequence Viewer

Length: 1146 bp
ATGGGTGCATTGGGAACTATTGCCATTGCACTTCTGGTGCTACCACTTCTACATGACCAAGTACATGGGAAGCATGCATACGAGAACTTGAACAAGTGTGATATTTACCAAGGAAGCTGGGTTTATGACGATTCCTATCCTCTTTATGATTCAAGTCTCTGCAACTTCATACAGAAGGAGTTCGAGTGCCAACAAAATGGGCGACCCGACAAACACTATCTCAAATATAGATGGCAACCCTCTTCATGCATGTTACCAAGGTGCGTTTTCTTAGTTCTTGCTAAATTGGGAAGAGAAGAATCGAACTCCCATCTCGGTGTTGGGGTAACTGTGTTTGATGGTGAAGATTTATTGGAAAGATTGAGAAATAAGCGCATCATGTTTGTTGGAGACTCGTTAAGTTTGAACCAGTGGCAATCTCTCACATGTATGCTTCATGCAGCCTCATCACAATCTAAGTACACTATTGAAAGAACAGGAAGCATCTCTACAATGACATTCCTGAGTCACAATGCTTCTTTGATGCTTGCACGCAATGCACTTCTTGTTGACATTGTAGAAGAAAGACAGGGGAGGGTTTTGAAGCTGGACTCCATTGATCATAAGGATCAGAAAGCATGGAAAGACATTGATATATTGATTTTCAACTCATGGCATTGGTGGCTCCACACAGGAAGGAAACAACCGTGGGATTTTATTCAAGATGGAGACAATTTACATGAAGACATGGATCGCCTTGTTGCTTATGAGAAAGCCTTAAAAACTTGGGCTCGATGGGTGGACAAAAATGTTGATCCGACTAAAACCAAAGTCTTCTTTCAAGGGATTTCACCCACTCATTGGAATTCAAGTTATTGGGGGTACCCTGAAGGGAAGAATTGCAGCAGGGAAACACAACCACTAATAGTGCCACCATACCCAAGAAGCCGACACCCGGCAGAAGGGATTGTGGAAAAAGTATTGAGCGACATGTCAAAGCCGGTGGGTTTGCTCAATGTAACAGGACTGTCCCGGCTAAGGAAAGATGCACACCCGTCCATATATGGCAACGGAGGGCACCGCGGCATGGACTGCAGCCACTGGTGTCTAGCTGGAGTTCCTGATACTTGGAACAGGCTGTTATACGCTGAAGTCATTCGACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

381

Amino Acids

43.81

Weight (kDa)

8.02

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 32 - 83 5.7e-23 PMR5 N terminal Domain
PC-Esterase PF13839 111 - 376 4.4e-92 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 861
AccB1I GGYRCC 2 cut(s) 861, 1056
AccB7I CCANNNNNTGG 1 cut(s) 840
AccII CGCG 1 cut(s) 1062
AciI CCGC 2 cut(s) 1060, 1062
AclWI GGATC 3 cut(s) 615, 738, 788
AcsI RAATTY 1 cut(s) 844
AcuI CTGAAG 1 cut(s) 888
AfaI GTAC 3 cut(s) 63, 461, 863
AfiI CCNNNNNNNGG 5 cut(s) 840, 934, 941, 1017, 1066
AflIII ACRYGT 2 cut(s) 425, 969
AgsI TTSAA 9 cut(s) 91, 153, 406, 470, 583, 646, 701, 821, 849
AluBI AGCT 3 cut(s) 117, 586, 1091
AluI AGCT 3 cut(s) 117, 586, 1091
Alw26I GTCTC 3 cut(s) 161, 384, 702
AlwI GGATC 3 cut(s) 615, 738, 788
AlwNI CAGNNNCTG 1 cut(s) 1080
ApeKI GCWGC 3 cut(s) 440, 882, 1074
ApoI RAATTY 1 cut(s) 844
Asp700I GAANNNNTTC 2 cut(s) 179, 1134
Asp718I GGTACC 1 cut(s) 861
AspLEI GCGC 1 cut(s) 375
AsuC2I CCSGG 2 cut(s) 935, 1012
AsuHPI GGTGA 2 cut(s) 353, 822
BaeGI GKGCMC 1 cut(s) 1059
BanI GGYRCC 2 cut(s) 861, 1056
BanII GRGCYC 1 cut(s) 772
BarI GAAGNNNNNNTAC 4 cut(s) 62, 94, 472, 504
BbsI GAAGAC 2 cut(s) 729, 805
BbvI GCAGC 3 cut(s) 452, 894, 1086
BccI CCATC 5 cut(s) 225, 318, 332, 698, 768
BclI TGATCA 1 cut(s) 598
BcnI CCSGG 2 cut(s) 935, 1012
BcoDI GTCTC 3 cut(s) 161, 384, 702
BfaI CTAG 1 cut(s) 1088
BfmI CTRYAG 1 cut(s) 1072
BisI GCNGC 4 cut(s) 441, 883, 1063, 1075
BlsI GCNGC 4 cut(s) 442, 884, 1064, 1076
Bme1390I CCNGG 2 cut(s) 935, 1012
BmiI GGNNCC 3 cut(s) 665, 863, 1058
BmrFI CCNGG 2 cut(s) 935, 1012
BmsI GCATC 4 cut(s) 384, 492, 513, 1015
BpiI GAAGAC 2 cut(s) 729, 805
BpmI CTGGAG 1 cut(s) 1113
Bpu10I CCTNAGC 1 cut(s) 1016
BpuMI CCSGG 2 cut(s) 935, 1012
BsaBI GATNNNNATC 1 cut(s) 135
BsaJI CCNNGG 4 cut(s) 109, 257, 686, 1060
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 5 cut(s) 840, 934, 941, 1017, 1066
Bse118I RCCGGY 1 cut(s) 979
Bse1I ACTGG 2 cut(s) 409, 1085
Bse3DI GCAATG 2 cut(s) 24, 541
Bse8I GATNNNNATC 1 cut(s) 135
BseDI CCNNGG 4 cut(s) 109, 257, 686, 1060
BseJI GATNNNNATC 1 cut(s) 135
BseLI CCNNNNNNNGG 5 cut(s) 840, 934, 941, 1017, 1066
BseMI GCAATG 2 cut(s) 24, 541
BseMII CTCAG 1 cut(s) 494
BseNI ACTGG 2 cut(s) 409, 1085
BseSI GKGCMC 1 cut(s) 1059
BseXI GCAGC 3 cut(s) 452, 894, 1086
BseYI CCCAGC 1 cut(s) 117
Bsh1236I CGCG 1 cut(s) 1062
BshNI GGYRCC 2 cut(s) 861, 1056
BsiSI CCGG 3 cut(s) 935, 980, 1012
BslFI GGGAC 1 cut(s) 994
BslI CCNNNNNNNGG 5 cut(s) 840, 934, 941, 1017, 1066
BsmAI GTCTC 3 cut(s) 161, 384, 702
BsmFI GGGAC 1 cut(s) 994
Bsp1286I GDGCHC 2 cut(s) 772, 1059
Bsp143I GATC 4 cut(s) 598, 607, 730, 793
BspACI CCGC 2 cut(s) 1060, 1062
BspCNI CTCAG 1 cut(s) 495
BspFNI CGCG 1 cut(s) 1062
BspLI GGNNCC 3 cut(s) 665, 863, 1058
BspMAI CTGCAG 1 cut(s) 1076
BspPI GGATC 3 cut(s) 615, 738, 788
BspT107I GGYRCC 2 cut(s) 861, 1056
BsrDI GCAATG 2 cut(s) 24, 541
BsrFI RCCGGY 1 cut(s) 979
BsrI ACTGG 2 cut(s) 409, 1085
BssAI RCCGGY 1 cut(s) 979
BssECI CCNNGG 4 cut(s) 109, 257, 686, 1060
BssMI GATC 4 cut(s) 598, 607, 730, 793
BssT1I CCWWGG 2 cut(s) 109, 257
Bst4CI ACNGT 3 cut(s) 331, 687, 1008
Bst6I CTCTTC 2 cut(s) 247, 286
BstAPI GCANNNNNTGC 2 cut(s) 536, 1071
BstC8I GCNNGC 3 cut(s) 75, 528, 532
BstDEI CTNAG 4 cut(s) 271, 456, 503, 1016
BstDSI CCRYGG 2 cut(s) 686, 1060
BstFNI CGCG 1 cut(s) 1062
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 4 cut(s) 601, 610, 733, 796
BstMAI GTCTC 3 cut(s) 161, 384, 702
BstMBI GATC 4 cut(s) 598, 607, 730, 793
BstMWI GCNNNNNNNGC 3 cut(s) 536, 661, 1071
BstNSI RCATGY 4 cut(s) 77, 253, 429, 973
BstSCI CCNGG 2 cut(s) 933, 1010
BstSFI CTRYAG 1 cut(s) 1072
BstSLI GKGCMC 1 cut(s) 1059
BstUI CGCG 1 cut(s) 1062
BstV1I GCAGC 3 cut(s) 452, 894, 1086
BstV2I GAAGAC 2 cut(s) 729, 805
BstXI CCANNNNNNTGG 2 cut(s) 65, 197
BtgI CCRYGG 2 cut(s) 686, 1060
BtsIMutI CAGTG 2 cut(s) 416, 1078
Cac8I GCNNGC 3 cut(s) 75, 528, 532
CaiI CAGNNNCTG 1 cut(s) 1080
CfoI GCGC 1 cut(s) 375
Cfr10I RCCGGY 1 cut(s) 979
Cfr42I CCGCGG 1 cut(s) 1063
Csp6I GTAC 3 cut(s) 62, 460, 862
CspCI CAANNNNNGTGG 2 cut(s) 963, 998
CviQI GTAC 3 cut(s) 62, 460, 862
DdeI CTNAG 4 cut(s) 271, 456, 503, 1016
DpnI GATC 4 cut(s) 600, 609, 732, 795
DpnII GATC 4 cut(s) 598, 607, 730, 793
Eam1104I CTCTTC 2 cut(s) 247, 286
EarI CTCTTC 2 cut(s) 247, 286
Eco130I CCWWGG 2 cut(s) 109, 257
Eco24I GRGCYC 1 cut(s) 772
Eco57I CTGAAG 1 cut(s) 888
EcoRI GAATTC 1 cut(s) 844
EcoT14I CCWWGG 2 cut(s) 109, 257
EcoT22I ATGCAT 2 cut(s) 79, 251
EcoT38I GRGCYC 1 cut(s) 772
ErhI CCWWGG 2 cut(s) 109, 257
FaqI GGGAC 1 cut(s) 994
FbaI TGATCA 1 cut(s) 598
Fnu4HI GCNGC 4 cut(s) 441, 883, 1063, 1075
FriOI GRGCYC 1 cut(s) 772
Fsp4HI GCNGC 4 cut(s) 441, 883, 1063, 1075
FspBI CTAG 1 cut(s) 1088
GlaI GCGC 1 cut(s) 374
GluI GCNGC 4 cut(s) 441, 883, 1063, 1075
GsaI CCCAGC 1 cut(s) 121
GsuI CTGGAG 1 cut(s) 1113
HapII CCGG 3 cut(s) 935, 980, 1012
HhaI GCGC 1 cut(s) 375
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HinfI GANTC 6 cut(s) 131, 149, 299, 392, 505, 590
HpaII CCGG 3 cut(s) 935, 980, 1012
HphI GGTGA 2 cut(s) 353, 822
Hpy166II GTNNAC 3 cut(s) 462, 550, 781
Hpy188I TCNGA 2 cut(s) 612, 798
Hpy188III TCNNGA 3 cut(s) 502, 701, 1100
Hpy8I GTNNAC 3 cut(s) 462, 550, 781
Hpy99I CGWCG 1 cut(s) 1143
HpyAV CCTTC 4 cut(s) 169, 669, 863, 935
HpyCH4III ACNGT 3 cut(s) 331, 687, 1008
HpyCH4IV ACGT 1 cut(s) 1141
HpyF10VI GCNNNNNNNGC 3 cut(s) 536, 661, 1071
HpyF3I CTNAG 4 cut(s) 271, 456, 503, 1016
HpySE526I ACGT 1 cut(s) 1141
HspAI GCGC 1 cut(s) 373
KpnI GGTACC 1 cut(s) 865
Ksp22I TGATCA 1 cut(s) 598
KspI CCGCGG 1 cut(s) 1063
Kzo9I GATC 4 cut(s) 598, 607, 730, 793
LmnI GCTCC 1 cut(s) 669
Lsp1109I GCAGC 3 cut(s) 452, 894, 1086
LweI GCATC 4 cut(s) 384, 492, 513, 1015
MaeI CTAG 1 cut(s) 1088
MaeII ACGT 1 cut(s) 1141
MaeIII GTNAC 4 cut(s) 252, 325, 506, 997
MalI GATC 4 cut(s) 600, 609, 732, 795
MboI GATC 4 cut(s) 598, 607, 730, 793
MboII GAAGA 8 cut(s) 234, 303, 308, 356, 572, 734, 805, 886
MhlI GDGCHC 2 cut(s) 772, 1059
MluCI AATT 4 cut(s) 284, 712, 844, 877
MlyI GAGTC 3 cut(s) 386, 514, 584
MmeI TCCRAC 2 cut(s) 367, 821
MnlI CCTC 5 cut(s) 150, 250, 454, 567, 1046
Mph1103I ATGCAT 2 cut(s) 79, 251
MroXI GAANNNNTTC 2 cut(s) 179, 1134
MseI TTAA 3 cut(s) 398, 758, 1144
MslI CAYNNNNRTG 2 cut(s) 315, 428
MspA1I CMGCKG 1 cut(s) 1062
MspI CCGG 3 cut(s) 935, 980, 1012
MspR9I CCNGG 2 cut(s) 935, 1012
MvnI CGCG 1 cut(s) 1062
MwoI GCNNNNNNNGC 3 cut(s) 536, 661, 1071
NciI CCSGG 2 cut(s) 935, 1012
NdeII GATC 4 cut(s) 598, 607, 730, 793
NlaIV GGNNCC 3 cut(s) 665, 863, 1058
NmuCI GTSAC 1 cut(s) 506
NsiI ATGCAT 2 cut(s) 79, 251
NspI RCATGY 4 cut(s) 77, 253, 429, 973
PaeI GCATGC 1 cut(s) 77
PciI ACATGT 2 cut(s) 425, 969
PdmI GAANNNNTTC 2 cut(s) 179, 1134
PfeI GAWTC 3 cut(s) 131, 149, 299
PflMI CCANNNNNTGG 1 cut(s) 840
PkrI GCNGC 4 cut(s) 442, 884, 1064, 1076
PleI GAGTC 3 cut(s) 386, 513, 584
PpsI GAGTC 3 cut(s) 386, 513, 584
PscI ACATGT 2 cut(s) 425, 969
PspFI CCCAGC 1 cut(s) 117
PspN4I GGNNCC 3 cut(s) 665, 863, 1058
PstI CTGCAG 1 cut(s) 1076
PstNI CAGNNNCTG 1 cut(s) 1080
RsaI GTAC 3 cut(s) 63, 461, 863
RsaNI GTAC 3 cut(s) 62, 460, 862
RseI CAYNNNNRTG 2 cut(s) 315, 428
SacII CCGCGG 1 cut(s) 1063
SaqAI TTAA 3 cut(s) 398, 758, 1144
SatI GCNGC 4 cut(s) 441, 883, 1063, 1075
Sau3AI GATC 4 cut(s) 598, 607, 730, 793
SchI GAGTC 3 cut(s) 386, 514, 584
ScrFI CCNGG 2 cut(s) 935, 1012
SduI GDGCHC 2 cut(s) 772, 1059
SetI ASST 5 cut(s) 119, 263, 588, 1093, 1144
SfaNI GCATC 4 cut(s) 384, 492, 513, 1015
SfcI CTRYAG 1 cut(s) 1072
Sfr303I CCGCGG 1 cut(s) 1063
SgrBI CCGCGG 1 cut(s) 1063
SmiMI CAYNNNNRTG 2 cut(s) 315, 428
SphI GCATGC 1 cut(s) 77
Sse9I AATT 4 cut(s) 284, 712, 844, 877
SsiI CCGC 2 cut(s) 1060, 1062
SspMI CTAG 1 cut(s) 1088
StyD4I CCNGG 2 cut(s) 933, 1010
StyI CCWWGG 2 cut(s) 109, 257
TaaI ACNGT 3 cut(s) 331, 687, 1008
TaiI ACGT 1 cut(s) 1144
TaqI TCGA 4 cut(s) 183, 302, 772, 1138
TasI AATT 4 cut(s) 284, 712, 844, 877
TatI WGTACW 2 cut(s) 61, 459
TauI GCSGC 1 cut(s) 1065
TfiI GAWTC 3 cut(s) 131, 149, 299
Tru1I TTAA 3 cut(s) 398, 758, 1144
Tru9I TTAA 3 cut(s) 398, 758, 1144
TscAI CASTG 2 cut(s) 416, 1085
TseFI GTSAC 1 cut(s) 506
TseI GCWGC 3 cut(s) 440, 882, 1074
Tsp45I GTSAC 1 cut(s) 506
TspDTI ATGAA 4 cut(s) 157, 234, 425, 735
TspGWI ACGGA 1 cut(s) 1065
TspRI CASTG 2 cut(s) 416, 1085
Van91I CCANNNNNTGG 1 cut(s) 840
XapI RAATTY 1 cut(s) 844
XceI RCATGY 4 cut(s) 77, 253, 429, 973
XcmI CCANNNNNNNNNTGG 2 cut(s) 31, 317
XmnI GAANNNNTTC 2 cut(s) 179, 1134
XspI CTAG 1 cut(s) 1088
Zsp2I ATGCAT 2 cut(s) 79, 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.