Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0000737

Physical Location & Seq

Genomic Coordinates Reverse (-)

46683880 .. 46684985

Gene Structure 1106 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G021880.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1056 bp

ATGAATACAATACTAGTTCTCATGCTTTTTGTTGTTTCGGCATACCAAGTACATAACACCGCCGGTTGCGATGTTTCAAACGGGAGCTGGGTTTATGATGACTCATATCCTCTCTATAACGCATCTAGCTGCTCCTTCATACTGAAAGCATTTGATTGTGTAGGTAATGGCCGCCCGGACAAAGATTATCTCAAATACAGATGGCAACCATCTGAGTGCAACTTACCGACTTTTAACGGCACTGATCTTCTAATGAGGTTCAAAGGGAAGAGCATCATGTTTGTCGGAGACTCGCTGAGTTCGAACCAATGGCAGTCACTAGCTTGCATGGTGTACACATCTATTACTCCACAGCCCAACTACACTTTGTTCAGGCAAGGCGAGCTCTCGACATTCGCGTTCCCGGAGTACGATGTCCAGCTCTTGTTCTCCCGGAATCCGCTTCTTGTGGATGTTGTTAGCACCAAGGTGGGACGAGTCCTTAAACTCGACTCCATCTCGCTCGAAAACCAGAAGCTGTGGAATGAAACCAGCGTAGTGATCTTTGACACGTGGCATTGGTGGCTTCACGTCGGAAGGAAGCAGCCATGGGATTTAATTCAGGTGGGGAACCAGACCTACGAGGACATGGATCGGTTGGTGGCATACGAAAAGGCTCTGAACACTTGGGCTAAGTGGGTGGACTCGAGCGTGGACCTTGCAAAAACCAAGGTCTTCTTCCAAGGAGTCTCCCCTGACCACCAGAATGCATCAGACTGGACGCTAAACCCTAAATCCGAAGCGATGAACTGCACTGCAGAATCCGAACCACTCCCTGCAGGTTCGGAAAACCCTAGTGCACATCCGGCAGAGCAAGTAGCGGAGAAAGTACTACGTAGCATGTTGAAGCCAGTCAGTTTGCTAAATGTCACAACTCTTTCACATCTAAGAAAGGATGGGCATCCCTCTGTGTATGGGCTTGGTGGGAGTAGAGGCTTGGACTGCACGCATTGGTGCTTATCTGGTGTTCCTGATACTTGGAATGTGCTTCTCTATGCAGCTCTTACTGAAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008374 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071554

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

351

Amino Acids

39.5

Weight (kDa)

5.49

Isoelectric Point (pI)

34.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	22 - 74	2.3e-23	PMR5 N terminal Domain
PC-Esterase	PF13839	75 - 346	7e-84	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000737)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g18101 FvH4_4g19380 FvH4_5g32790 FvH4_5g32790 FvH4_6g27901 FvH4_6g37530
malus_domestica	MD09G1233400.v1.1 MD09G1233600.v1.1 MD17G1143400.v1.1 MD17G1229900.v1.1 MD17G1230000.v1.1
prunus_persica	Prupe.3G108000_v2.0.a1 Prupe.3G108000_v2.0.a1 Prupe.3G108200_v2.0.a1 Prupe.3G108300_v2.0.a1 Prupe.3G108300_v2.0.a1 Prupe.3G108300_v2.0.a1 Prupe.3G108300_v2.0.a1 Prupe.3G168700_v2.0.a1
pyrus_communis	pycom09g15180 pycom17g23260 pycom17g23270
rosa_chinensis	RchiOBHm_Chr2g0131401 RchiOBHm_Chr2g0131411 RchiOBHm_Chr2g0131441 RchiOBHm_Chr2g0150421 RchiOBHm_Chr4g0424421
rosa_laevigata	RLG00000007476 RLG00000019182 RLG00000019183 RLG00000020497
rosa_multiflora	Rmu_sc0003445.1_g000007 Rmu_sc0003567.1_g000013 Rmu_sc0005652.1_g000008 Rmu_sc0005652.1_g000023 Rmu_sc0005652.1_g000030 Rmu_sc0016958.1_g000002 Rmu_sc0043021.1_g000001 Rmu_ssc0000480.1_g000015
rosa_roxburghii	Rroxscaffold_2G00097140 Rroxscaffold_2G00112870 Rroxscaffold_2G00112970 Rroxscaffold_2G00112980 Rroxscaffold_5G00366360
rosa_rugosa	Rorug02G0296600 Rorug02G0296700 Rorug02G0421100 Rorug02G0421200 Rorug04G0197700
rosa_samantha	Rh2AG350000 Rh2AG350400 Rh2AG480500 Rh2BG356700 Rh2BG356800 Rh2BG492600 Rh2CG335500 Rh2CG335600 Rh2CG335700 Rh2CG335900 Rh2CG466800 Rh2DG374400 Rh2DG501400 Rh4AG253500 Rh4BG258900 Rh4CG270200 Rh4DG253500
rosa_wichuraiana	Rw2G028380 Rw2G028390 Rw2G028420 Rw2G039220 Rw4G021880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	809
AccII	CGCG	1 cut(s)	398
AciI	CCGC	4 cut(s)	60, 172, 440, 860
AclWI	GGATC	1 cut(s)	639
AcoI	YGGCCR	1 cut(s)	169
AcvI	CACGTG	1 cut(s)	552
AfaI	GTAC	4 cut(s)	51, 335, 410, 870
AflIII	ACRYGT	1 cut(s)	549
AgsI	TTSAA	3 cut(s)	78, 262, 886
AhlI	ACTAGT	1 cut(s)	13
AjiI	CACGTC	1 cut(s)	571
AjuI	GAANNNNNNNTTGG	2 cut(s)	701, 733
AleI	CACNNNNGTG	1 cut(s)	467
AluBI	AGCT	7 cut(s)	87, 129, 323, 385, 421, 517, 1040
AluI	AGCT	7 cut(s)	87, 129, 323, 385, 421, 517, 1040
Alw21I	GWGCWC	2 cut(s)	387, 841
Alw26I	GTCTC	2 cut(s)	282, 733
Alw44I	GTGCAC	1 cut(s)	837
AlwI	GGATC	1 cut(s)	639
AlwNI	CAGNNNCTG	1 cut(s)	517
Ama87I	CYCGRG	1 cut(s)	685
AoxI	GGCC	1 cut(s)	169
ApaLI	GTGCAC	1 cut(s)	837
ApeKI	GCWGC	3 cut(s)	129, 583, 1037
AspS9I	GGNCC	1 cut(s)	694
AsuC2I	CCSGG	3 cut(s)	176, 404, 433
AsuII	TTCGAA	1 cut(s)	302
AvaI	CYCGRG	1 cut(s)	685
AvaII	GGWCC	1 cut(s)	694
BaeGI	GKGCMC	1 cut(s)	841
BanII	GRGCYC	1 cut(s)	387
BbrPI	CACGTG	1 cut(s)	552
BbsI	GAAGAC	1 cut(s)	706
Bbv12I	GWGCWC	2 cut(s)	387, 841
BbvI	GCAGC	3 cut(s)	116, 595, 1049
BccI	CCATC	4 cut(s)	195, 217, 503, 929
BceAI	ACGGC	1 cut(s)	253
BcnI	CCSGG	3 cut(s)	176, 404, 433
BcoDI	GTCTC	2 cut(s)	282, 733
BcuI	ACTAGT	1 cut(s)	13
BfaI	CTAG	4 cut(s)	14, 126, 320, 834
BfmI	CTRYAG	2 cut(s)	795, 816
BfuAI	ACCTGC	1 cut(s)	809
BisI	GCNGC	4 cut(s)	130, 172, 584, 1038
BlsI	GCNGC	4 cut(s)	131, 173, 585, 1039
BmcAI	AGTACT	1 cut(s)	870
Bme1390I	CCNGG	3 cut(s)	176, 404, 433
Bme18I	GGWCC	1 cut(s)	694
BmeT110I	CYCGRG	1 cut(s)	685
BmgBI	CACGTC	1 cut(s)	571
BmgT120I	GGNCC	1 cut(s)	694
BmiI	GGNNCC	1 cut(s)	611
BmrFI	CCNGG	3 cut(s)	176, 404, 433
BmsI	GCATC	4 cut(s)	131, 282, 758, 949
BpiI	GAAGAC	1 cut(s)	706
Bpu14I	TTCGAA	1 cut(s)	302
BpuMI	CCSGG	3 cut(s)	176, 404, 433
BsaAI	YACGTR	2 cut(s)	552, 875
BsaBI	GATNNNNATC	1 cut(s)	939
BsaJI	CCNNGG	4 cut(s)	465, 587, 708, 721
Bse118I	RCCGGY	1 cut(s)	62
Bse1I	ACTGG	2 cut(s)	761, 890
Bse8I	GATNNNNATC	1 cut(s)	939
BseDI	CCNNGG	4 cut(s)	465, 587, 708, 721
BseGI	GGATG	4 cut(s)	457, 841, 940, 940
BseJI	GATNNNNATC	1 cut(s)	939
BseMII	CTCAG	2 cut(s)	204, 287
BseNI	ACTGG	2 cut(s)	761, 890
BseSI	GKGCMC	1 cut(s)	841
BseXI	GCAGC	3 cut(s)	116, 595, 1049
BseYI	CCCAGC	1 cut(s)	87
BsgI	GTGCAG	2 cut(s)	775, 967
Bsh1236I	CGCG	1 cut(s)	398
BshFI	GGCC	1 cut(s)	171
BsiHKAI	GWGCWC	2 cut(s)	387, 841
BsiHKCI	CYCGRG	1 cut(s)	685
BsiSI	CCGG	5 cut(s)	63, 176, 404, 433, 845
BslFI	GGGAC	1 cut(s)	486
BsmAI	GTCTC	2 cut(s)	282, 733
BsmFI	GGGAC	1 cut(s)	486
BsmI	GAATGC	1 cut(s)	751
BsnI	GGCC	1 cut(s)	171
BsoBI	CYCGRG	1 cut(s)	685
Bsp119I	TTCGAA	1 cut(s)	302
Bsp1286I	GDGCHC	2 cut(s)	387, 841
Bsp1407I	TGTACA	1 cut(s)	333
Bsp143I	GATC	3 cut(s)	244, 540, 631
Bsp19I	CCATGG	1 cut(s)	587
BspACI	CCGC	4 cut(s)	60, 172, 440, 860
BspANI	GGCC	1 cut(s)	171
BspCNI	CTCAG	2 cut(s)	205, 288
BspFNI	CGCG	1 cut(s)	398
BspLI	GGNNCC	1 cut(s)	611
BspMAI	CTGCAG	2 cut(s)	799, 820
BspMI	ACCTGC	1 cut(s)	809
BspPI	GGATC	1 cut(s)	639
BspQI	GCTCTTC	1 cut(s)	263
BspT104I	TTCGAA	1 cut(s)	302
BsrFI	RCCGGY	1 cut(s)	62
BsrGI	TGTACA	1 cut(s)	333
BsrI	ACTGG	2 cut(s)	761, 890
BssAI	RCCGGY	1 cut(s)	62
BssECI	CCNNGG	4 cut(s)	465, 587, 708, 721
BssMI	GATC	3 cut(s)	244, 540, 631
BssT1I	CCWWGG	4 cut(s)	465, 587, 708, 721
Bst6I	CTCTTC	1 cut(s)	263
BstAUI	TGTACA	1 cut(s)	333
BstBAI	YACGTR	2 cut(s)	552, 875
BstBI	TTCGAA	1 cut(s)	302
BstC8I	GCNNGC	3 cut(s)	325, 383, 986
BstDEI	CTNAG	4 cut(s)	213, 296, 672, 926
BstDSI	CCRYGG	1 cut(s)	587
BstF5I	GGATG	4 cut(s)	457, 841, 940, 940
BstFNI	CGCG	1 cut(s)	398
BstKTI	GATC	3 cut(s)	247, 543, 634
BstMAI	GTCTC	2 cut(s)	282, 733
BstMBI	GATC	3 cut(s)	244, 540, 631
BstMWI	GCNNNNNNNGC	4 cut(s)	382, 562, 845, 981
BstNSI	RCATGY	1 cut(s)	883
BstSCI	CCNGG	3 cut(s)	174, 402, 431
BstSFI	CTRYAG	2 cut(s)	795, 816
BstSLI	GKGCMC	1 cut(s)	841
BstSNI	TACGTA	1 cut(s)	875
BstUI	CGCG	1 cut(s)	398
BstV1I	GCAGC	3 cut(s)	116, 595, 1049
BstV2I	GAAGAC	1 cut(s)	706
BsuRI	GGCC	1 cut(s)	171
BtgI	CCRYGG	1 cut(s)	587
BtgZI	GCGATG	2 cut(s)	84, 797
BtrI	CACGTC	1 cut(s)	571
BtsCI	GGATG	4 cut(s)	457, 841, 940, 940
BtsI	GCAGTG	1 cut(s)	792
BtsIMutI	CAGTG	2 cut(s)	240, 792
BveI	ACCTGC	1 cut(s)	809
Cac8I	GCNNGC	3 cut(s)	325, 383, 986
CaiI	CAGNNNCTG	1 cut(s)	517
Cfr10I	RCCGGY	1 cut(s)	62
Cfr13I	GGNCC	1 cut(s)	694
CseI	GACGC	1 cut(s)	769
Csp6I	GTAC	4 cut(s)	50, 334, 409, 869
CviAII	CATG	6 cut(s)	22, 277, 328, 588, 628, 880
CviQI	GTAC	4 cut(s)	50, 334, 409, 869
DdeI	CTNAG	4 cut(s)	213, 296, 672, 926
DpnI	GATC	3 cut(s)	246, 542, 633
DpnII	GATC	3 cut(s)	244, 540, 631
EaeI	YGGCCR	1 cut(s)	169
Eam1104I	CTCTTC	1 cut(s)	263
EarI	CTCTTC	1 cut(s)	263
Ecl136II	GAGCTC	1 cut(s)	385
Eco105I	TACGTA	1 cut(s)	875
Eco130I	CCWWGG	4 cut(s)	465, 587, 708, 721
Eco24I	GRGCYC	1 cut(s)	387
Eco47I	GGWCC	1 cut(s)	694
Eco53kI	GAGCTC	1 cut(s)	385
Eco72I	CACGTG	1 cut(s)	552
Eco88I	CYCGRG	1 cut(s)	685
EcoICRI	GAGCTC	1 cut(s)	385
EcoT14I	CCWWGG	4 cut(s)	465, 587, 708, 721
EcoT22I	ATGCAT	1 cut(s)	751
EcoT38I	GRGCYC	1 cut(s)	387
ErhI	CCWWGG	4 cut(s)	465, 587, 708, 721
FaeI	CATG	6 cut(s)	25, 280, 331, 591, 631, 883
FalI	AAGNNNNNCTT	2 cut(s)	701, 733
FaqI	GGGAC	1 cut(s)	486
FatI	CATG	6 cut(s)	21, 276, 327, 587, 627, 879
Fnu4HI	GCNGC	4 cut(s)	130, 172, 584, 1038
FokI	GGATG	4 cut(s)	464, 828, 927, 947
FriOI	GRGCYC	1 cut(s)	387
Fsp4HI	GCNGC	4 cut(s)	130, 172, 584, 1038
FspBI	CTAG	4 cut(s)	14, 126, 320, 834
GluI	GCNGC	4 cut(s)	130, 172, 584, 1038
GsaI	CCCAGC	1 cut(s)	91
HaeIII	GGCC	1 cut(s)	171
HapII	CCGG	5 cut(s)	63, 176, 404, 433, 845
HgaI	GACGC	1 cut(s)	769
Hin1II	CATG	6 cut(s)	25, 280, 331, 591, 631, 883
HinfI	GANTC	8 cut(s)	101, 290, 436, 477, 491, 683, 726, 800
HpaII	CCGG	5 cut(s)	63, 176, 404, 433, 845
Hpy166II	GTNNAC	5 cut(s)	334, 336, 682, 694, 839
Hpy188I	TCNGA	8 cut(s)	214, 287, 575, 660, 754, 778, 805, 826
Hpy188III	TCNNGA	2 cut(s)	388, 1010
Hpy8I	GTNNAC	5 cut(s)	334, 336, 682, 694, 839
Hpy99I	CGWCG	1 cut(s)	575
HpyAV	CCTTC	2 cut(s)	145, 570
HpyCH4IV	ACGT	3 cut(s)	551, 570, 874
HpyF10VI	GCNNNNNNNGC	4 cut(s)	382, 562, 845, 981
HpyF3I	CTNAG	4 cut(s)	213, 296, 672, 926
HpySE526I	ACGT	3 cut(s)	551, 570, 874
Hsp92II	CATG	6 cut(s)	25, 280, 331, 591, 631, 883
Kzo9I	GATC	3 cut(s)	244, 540, 631
LguI	GCTCTTC	1 cut(s)	263
LmnI	GCTCC	2 cut(s)	84, 137
Lsp1109I	GCAGC	3 cut(s)	116, 595, 1049
LweI	GCATC	4 cut(s)	131, 282, 758, 949
MaeI	CTAG	4 cut(s)	14, 126, 320, 834
MaeII	ACGT	3 cut(s)	551, 570, 874
MaeIII	GTNAC	2 cut(s)	315, 907
MalI	GATC	3 cut(s)	246, 542, 633
MboI	GATC	3 cut(s)	244, 540, 631
MboII	GAAGA	4 cut(s)	239, 280, 706, 709
MhlI	GDGCHC	2 cut(s)	387, 841
MluCI	AATT	1 cut(s)	597
MlyI	GAGTC	6 cut(s)	95, 284, 485, 486, 677, 735
MmeI	TCCRAC	2 cut(s)	265, 553
MnlI	CCTC	5 cut(s)	120, 249, 616, 955, 965
Mph1103I	ATGCAT	1 cut(s)	751
MseI	TTAA	4 cut(s)	234, 483, 596, 1054
MslI	CAYNNNNRTG	3 cut(s)	214, 467, 744
MspI	CCGG	5 cut(s)	63, 176, 404, 433, 845
MspR9I	CCNGG	3 cut(s)	176, 404, 433
Mva1269I	GAATGC	1 cut(s)	751
MvnI	CGCG	1 cut(s)	398
MwoI	GCNNNNNNNGC	4 cut(s)	382, 562, 845, 981
NciI	CCSGG	3 cut(s)	176, 404, 433
NcoI	CCATGG	1 cut(s)	587
NdeII	GATC	3 cut(s)	244, 540, 631
NlaIII	CATG	6 cut(s)	25, 280, 331, 591, 631, 883
NlaIV	GGNNCC	1 cut(s)	611
NmuCI	GTSAC	2 cut(s)	315, 907
NsiI	ATGCAT	1 cut(s)	751
NspI	RCATGY	1 cut(s)	883
NspV	TTCGAA	1 cut(s)	302
OliI	CACNNNNGTG	1 cut(s)	467
PaeR7I	CTCGAG	1 cut(s)	685
PciSI	GCTCTTC	1 cut(s)	263
PcsI	WCGNNNNNNNCGW	2 cut(s)	299, 395
PctI	GAATGC	1 cut(s)	751
PfeI	GAWTC	2 cut(s)	436, 800
PfoI	TCCNGGA	2 cut(s)	402, 431
PkrI	GCNGC	4 cut(s)	131, 173, 585, 1039
PleI	GAGTC	6 cut(s)	95, 284, 485, 485, 677, 734
PmaCI	CACGTG	1 cut(s)	552
PmlI	CACGTG	1 cut(s)	552
PpsI	GAGTC	6 cut(s)	95, 284, 485, 485, 677, 734
Ppu21I	YACGTR	2 cut(s)	552, 875
Psp124BI	GAGCTC	1 cut(s)	387
PspCI	CACGTG	1 cut(s)	552
PspFI	CCCAGC	1 cut(s)	87
PspN4I	GGNNCC	1 cut(s)	611
PspPI	GGNCC	1 cut(s)	694
PspXI	VCTCGAGB	1 cut(s)	685
PsrI	GAACNNNNNNTAC	2 cut(s)	602, 634
PstI	CTGCAG	2 cut(s)	799, 820
PstNI	CAGNNNCTG	1 cut(s)	517
RsaI	GTAC	4 cut(s)	51, 335, 410, 870
RsaNI	GTAC	4 cut(s)	50, 334, 409, 869
RseI	CAYNNNNRTG	3 cut(s)	214, 467, 744
SacI	GAGCTC	1 cut(s)	387
SapI	GCTCTTC	1 cut(s)	263
SaqAI	TTAA	4 cut(s)	234, 483, 596, 1054
SatI	GCNGC	4 cut(s)	130, 172, 584, 1038
Sau3AI	GATC	3 cut(s)	244, 540, 631
Sau96I	GGNCC	1 cut(s)	694
SbfI	CCTGCAGG	1 cut(s)	820
ScaI	AGTACT	1 cut(s)	870
SchI	GAGTC	6 cut(s)	95, 284, 485, 486, 677, 735
ScrFI	CCNGG	3 cut(s)	176, 404, 433
SdaI	CCTGCAGG	1 cut(s)	820
SduI	GDGCHC	2 cut(s)	387, 841
SfaNI	GCATC	4 cut(s)	131, 282, 758, 949
SfcI	CTRYAG	2 cut(s)	795, 816
Sfr274I	CTCGAG	1 cut(s)	685
SfuI	TTCGAA	1 cut(s)	302
SinI	GGWCC	1 cut(s)	694
SlaI	CTCGAG	1 cut(s)	685
SmiMI	CAYNNNNRTG	3 cut(s)	214, 467, 744
SmlI	CTYRAG	1 cut(s)	685
SmoI	CTYRAG	1 cut(s)	685
SnaBI	TACGTA	1 cut(s)	875
SpeI	ACTAGT	1 cut(s)	13
Sse8387I	CCTGCAGG	1 cut(s)	820
Sse9I	AATT	1 cut(s)	597
SsiI	CCGC	4 cut(s)	60, 172, 440, 860
SspMI	CTAG	4 cut(s)	14, 126, 320, 834
SstI	GAGCTC	1 cut(s)	387
StyD4I	CCNGG	3 cut(s)	174, 402, 431
StyI	CCWWGG	4 cut(s)	465, 587, 708, 721
TaiI	ACGT	3 cut(s)	554, 573, 877
TaqI	TCGA	5 cut(s)	302, 389, 489, 504, 686
TasI	AATT	1 cut(s)	597
TatI	WGTACW	3 cut(s)	49, 333, 868
TauI	GCSGC	1 cut(s)	174
TfiI	GAWTC	2 cut(s)	436, 800
Tru1I	TTAA	4 cut(s)	234, 483, 596, 1054
Tru9I	TTAA	4 cut(s)	234, 483, 596, 1054
TscAI	CASTG	2 cut(s)	247, 799
TseFI	GTSAC	2 cut(s)	315, 907
TseI	GCWGC	3 cut(s)	129, 583, 1037
Tsp45I	GTSAC	2 cut(s)	315, 907
TspDTI	ATGAA	4 cut(s)	17, 127, 540, 800
TspRI	CASTG	2 cut(s)	247, 799
VneI	GTGCAC	1 cut(s)	837
VpaK11BI	GGWCC	1 cut(s)	694
XceI	RCATGY	1 cut(s)	883
XhoI	CTCGAG	1 cut(s)	685
XspI	CTAG	4 cut(s)	14, 126, 320, 834
ZrmI	AGTACT	1 cut(s)	870
Zsp2I	ATGCAT	1 cut(s)	751

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.