MD17G1143400.v1.1

Protein trichome birefringence-like 43

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000737
Physical Location & Seq
Reverse (-)
12929305 .. 12931178
1874 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1143400.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGGGTGCATTTGGCATTGCCATTGCACTTCTAGTGCTACCAATAATTTTAGAAAACCAAATTGTATGCGGGAAGCAAAGCATTTACAGGTTGAAGAAGTGTGATATTTACCAAGGAAGATGGGTTTATGACCCTAATTCTTACCCTCTTTACGATTCAAGTCTTTGCCACTTCTTAGATCATGCCTTCGATTGCCAGAAAAATGGTCGAGCCGACGAACTCTATCTCAAATATCGATGGCAACCCTATTCATGCAATTTACCAACGTTTGATGGTGAAGATTTCTTGGAGAGATTTAGAGATAAACGCATCATGTTTGTTGGAGACTCGTTAAGTTTGAACCAATGGCAATCGCTCACGTGCATGATTCATGCAACTTCACCTCAGTCCGGTTATACCATCAAGAGGATAGGCGGAACCTCTACGTTCACATCGAAGAGTCACAATGTTTCTTTGATGCTAGCACGCAATGCATTTCTGGTTCACATTGATCAACAGAGATATGGTAGGGTTTTGAAACTCGACTCTATCGACCTTAAGGATGGGAAATCATGGAAAGAATATGATGTTTTGATCTTCAACACTTGGCATTGGTGGCTCCACGCTAGAAGGAAACAACCGTGGGATTTTATTCAAGATGGAAAGACTTTACATCGAGATATGAATCGTCTTGTTGCTTATGAGAAAGCCTTGAAAACTTGGGCTCGTTGGGTGGACAAAAATGTTGATACTAAGAAAACCAAAGTCTTCTTCCAGAGTATTTCACCGAGTCATTGGAATGCAAGTCATTGGGGTTACCCAGATGGGAGGAACTGCACAGCAGAAAGAGAGCCACTAGTAGTAGCATACCCAAGAAGCAAAGACCCAGCAGAAGTGATTGTGGAAAAAGTGCTTGGTGACATGTCGAAGCAAGTGCTTTTGCTTAACGTGACAGGGCTGTCACAGCTGAGGAAAGACGGTCACCCATCTATATATGGCAATGGAGCACACCTTGGCATGGACTGCAGCCATTGGTGCCTCCCTGGAGTTCCTGACACATGGAACCAGTTGCTGTACACTGAGCTCATTCGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

359

Amino Acids

41.57

Weight (kDa)

8.91

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 32 - 86 2.9e-22 PMR5 N terminal Domain
PC-Esterase PF13839 87 - 353 4.6e-92 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 937
AccB1I GGYRCC 1 cut(s) 1014
AciI CCGC 2 cut(s) 69, 414
AclI AACGTT 1 cut(s) 266
AcvI CACGTG 1 cut(s) 360
AfaI GTAC 1 cut(s) 1055
AfiI CCNNNNNNNGG 3 cut(s) 389, 405, 997
AflII CTTAAG 1 cut(s) 536
AflIII ACRYGT 1 cut(s) 900
AgsI TTSAA 7 cut(s) 94, 159, 340, 517, 580, 635, 694
AhlI ACTAGT 1 cut(s) 835
AjnI CCWGG 1 cut(s) 1021
AjuI GAANNNNNNNTTGG 4 cut(s) 734, 766, 876, 908
AluBI AGCT 2 cut(s) 946, 1063
AluI AGCT 2 cut(s) 946, 1063
Alw21I GWGCWC 2 cut(s) 988, 1065
Alw26I GTCTC 1 cut(s) 318
AlwNI CAGNNNCTG 1 cut(s) 1051
ApeKI GCWGC 1 cut(s) 1005
AsuHPI GGTGA 5 cut(s) 287, 372, 756, 908, 953
AsuII TTCGAA 1 cut(s) 1069
AsuNHI GCTAGC 1 cut(s) 460
BanI GGYRCC 1 cut(s) 1014
BanII GRGCYC 2 cut(s) 706, 1065
BbrPI CACGTG 1 cut(s) 360
BbsI GAAGAC 1 cut(s) 739
Bbv12I GWGCWC 2 cut(s) 988, 1065
BbvCI CCTCAGC 1 cut(s) 947
BbvI GCAGC 1 cut(s) 1017
BccI CCATC 8 cut(s) 114, 231, 266, 407, 536, 632, 797, 973
BcgI CGANNNNNNTGC 2 cut(s) 895, 929
BciT130I CCWGG 1 cut(s) 1023
BclI TGATCA 1 cut(s) 490
BcoDI GTCTC 1 cut(s) 318
BcuI ACTAGT 1 cut(s) 835
BfaI CTAG 4 cut(s) 32, 461, 606, 836
BfmI CTRYAG 1 cut(s) 1003
BfrI CTTAAG 1 cut(s) 536
BisI GCNGC 1 cut(s) 1006
BlsI GCNGC 1 cut(s) 1007
Bme1390I CCNGG 1 cut(s) 1023
BmiI GGNNCC 4 cut(s) 418, 599, 1016, 1043
BmrFI CCNGG 1 cut(s) 1023
BmsI GCATC 2 cut(s) 318, 447
BmtI GCTAGC 1 cut(s) 464
BpiI GAAGAC 1 cut(s) 739
BpmI CTGGAG 1 cut(s) 1044
Bpu10I CCTNAGC 1 cut(s) 947
Bpu14I TTCGAA 1 cut(s) 1069
Bsa29I ATCGAT 1 cut(s) 235
BsaAI YACGTR 1 cut(s) 360
BsaBI GATNNNNATC 1 cut(s) 663
BsaJI CCNNGG 4 cut(s) 112, 620, 991, 1021
BsaWI WCCGGW 1 cut(s) 389
Bsc4I CCNNNNNNNGG 3 cut(s) 389, 405, 997
Bse1I ACTGG 1 cut(s) 1045
Bse3DI GCAATG 4 cut(s) 15, 21, 475, 985
Bse8I GATNNNNATC 1 cut(s) 663
BseBI CCWGG 1 cut(s) 1023
BseCI ATCGAT 1 cut(s) 235
BseDI CCNNGG 4 cut(s) 112, 620, 991, 1021
BseGI GGATG 1 cut(s) 547
BseJI GATNNNNATC 1 cut(s) 663
BseLI CCNNNNNNNGG 3 cut(s) 389, 405, 997
BseMI GCAATG 4 cut(s) 15, 21, 475, 985
BseMII CTCAG 3 cut(s) 398, 938, 1050
BseNI ACTGG 1 cut(s) 1045
BseXI GCAGC 1 cut(s) 1017
BseYI CCCAGC 1 cut(s) 865
BsgI GTGCAG 1 cut(s) 799
BshNI GGYRCC 1 cut(s) 1014
BshVI ATCGAT 1 cut(s) 235
BsiHKAI GWGCWC 2 cut(s) 988, 1065
BsiSI CCGG 1 cut(s) 390
BslI CCNNNNNNNGG 3 cut(s) 389, 405, 997
BsmAI GTCTC 1 cut(s) 318
BsmI GAATGC 1 cut(s) 784
Bsp119I TTCGAA 1 cut(s) 1069
Bsp1286I GDGCHC 3 cut(s) 706, 988, 1065
Bsp1407I TGTACA 1 cut(s) 1053
Bsp143I GATC 3 cut(s) 178, 490, 573
BspACI CCGC 2 cut(s) 69, 414
BspCNI CTCAG 3 cut(s) 397, 939, 1051
BspDI ATCGAT 1 cut(s) 235
BspLI GGNNCC 4 cut(s) 418, 599, 1016, 1043
BspMAI CTGCAG 1 cut(s) 1007
BspOI GCTAGC 1 cut(s) 464
BspT104I TTCGAA 1 cut(s) 1069
BspT107I GGYRCC 1 cut(s) 1014
BspTI CTTAAG 1 cut(s) 536
BsrDI GCAATG 4 cut(s) 15, 21, 475, 985
BsrGI TGTACA 1 cut(s) 1053
BsrI ACTGG 1 cut(s) 1045
BssECI CCNNGG 4 cut(s) 112, 620, 991, 1021
BssMI GATC 3 cut(s) 178, 490, 573
BssT1I CCWWGG 2 cut(s) 112, 991
Bst2UI CCWGG 1 cut(s) 1023
Bst4CI ACNGT 2 cut(s) 621, 959
Bst6I CTCTTC 1 cut(s) 431
BstAFI CTTAAG 1 cut(s) 536
BstAPI GCANNNNNTGC 2 cut(s) 470, 1002
BstAUI TGTACA 1 cut(s) 1053
BstBAI YACGTR 1 cut(s) 360
BstBI TTCGAA 1 cut(s) 1069
BstC8I GCNNGC 2 cut(s) 462, 466
BstDEI CTNAG 5 cut(s) 175, 384, 732, 947, 1059
BstDSI CCRYGG 1 cut(s) 620
BstEII GGTNACC 2 cut(s) 794, 959
BstF5I GGATG 1 cut(s) 547
BstKTI GATC 3 cut(s) 181, 493, 576
BstMAI GTCTC 1 cut(s) 318
BstMBI GATC 3 cut(s) 178, 490, 573
BstMWI GCNNNNNNNGC 5 cut(s) 470, 595, 943, 1002, 1014
BstNI CCWGG 1 cut(s) 1023
BstNSI RCATGY 1 cut(s) 904
BstPI GGTNACC 2 cut(s) 794, 959
BstSCI CCNGG 1 cut(s) 1021
BstSFI CTRYAG 1 cut(s) 1003
BstV1I GCAGC 1 cut(s) 1017
BstV2I GAAGAC 1 cut(s) 739
BstXI CCANNNNNNTGG 1 cut(s) 203
Bsu15I ATCGAT 1 cut(s) 235
BsuTUI ATCGAT 1 cut(s) 235
BtgI CCRYGG 1 cut(s) 620
BtsCI GGATG 1 cut(s) 547
BtsIMutI CAGTG 1 cut(s) 1056
Cac8I GCNNGC 2 cut(s) 462, 466
CaiI CAGNNNCTG 1 cut(s) 1051
ClaI ATCGAT 1 cut(s) 235
Csp6I GTAC 1 cut(s) 1054
CviAII CATG 9 cut(s) 182, 252, 313, 364, 371, 552, 901, 997, 1038
CviJI RGCY 9 cut(s) 212, 598, 689, 704, 832, 937, 946, 1008, 1063
CviKI_1 RGCY 9 cut(s) 212, 598, 689, 704, 832, 937, 946, 1008, 1063
CviQI GTAC 1 cut(s) 1054
DdeI CTNAG 5 cut(s) 175, 384, 732, 947, 1059
DpnI GATC 3 cut(s) 180, 492, 575
DpnII GATC 3 cut(s) 178, 490, 573
DrdI GACNNNNNNGTC 1 cut(s) 937
DseDI GACNNNNNNGTC 1 cut(s) 937
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
EciI GGCGGA 1 cut(s) 429
Ecl136II GAGCTC 1 cut(s) 1063
Eco130I CCWWGG 2 cut(s) 112, 991
Eco24I GRGCYC 2 cut(s) 706, 1065
Eco53kI GAGCTC 1 cut(s) 1063
Eco72I CACGTG 1 cut(s) 360
Eco91I GGTNACC 2 cut(s) 794, 959
EcoICRI GAGCTC 1 cut(s) 1063
EcoO65I GGTNACC 2 cut(s) 794, 959
EcoRII CCWGG 1 cut(s) 1021
EcoT14I CCWWGG 2 cut(s) 112, 991
EcoT22I ATGCAT 1 cut(s) 475
EcoT38I GRGCYC 2 cut(s) 706, 1065
ErhI CCWWGG 2 cut(s) 112, 991
FaeI CATG 9 cut(s) 185, 255, 316, 367, 374, 555, 904, 1000, 1041
FatI CATG 9 cut(s) 181, 251, 312, 363, 370, 551, 900, 996, 1037
FauI CCCGC 1 cut(s) 62
FbaI TGATCA 1 cut(s) 490
Fnu4HI GCNGC 1 cut(s) 1006
FokI GGATG 1 cut(s) 554
FriOI GRGCYC 2 cut(s) 706, 1065
Fsp4HI GCNGC 1 cut(s) 1006
FspBI CTAG 4 cut(s) 32, 461, 606, 836
GluI GCNGC 1 cut(s) 1006
GsaI CCCAGC 1 cut(s) 869
GsuI CTGGAG 1 cut(s) 1044
HapII CCGG 1 cut(s) 390
Hin1II CATG 9 cut(s) 185, 255, 316, 367, 374, 555, 904, 1000, 1041
HinfI GANTC 7 cut(s) 155, 326, 367, 439, 524, 664, 769
HpaII CCGG 1 cut(s) 390
HphI GGTGA 5 cut(s) 287, 372, 756, 908, 953
Hpy166II GTNNAC 4 cut(s) 429, 484, 715, 1056
Hpy188III TCNNGA 5 cut(s) 403, 635, 656, 754, 1031
Hpy8I GTNNAC 4 cut(s) 429, 484, 715, 1056
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 2 cut(s) 196, 603
HpyCH4III ACNGT 2 cut(s) 621, 959
HpyCH4IV ACGT 4 cut(s) 266, 359, 425, 927
HpyCH4V TGCA 9 cut(s) 8, 26, 255, 363, 374, 473, 782, 816, 1005
HpyF10VI GCNNNNNNNGC 5 cut(s) 470, 595, 943, 1002, 1014
HpyF3I CTNAG 5 cut(s) 175, 384, 732, 947, 1059
HpySE526I ACGT 4 cut(s) 266, 359, 425, 927
Hsp92II CATG 9 cut(s) 185, 255, 316, 367, 374, 555, 904, 1000, 1041
Ksp22I TGATCA 1 cut(s) 490
Kzo9I GATC 3 cut(s) 178, 490, 573
LmnI GCTCC 2 cut(s) 603, 983
Lsp1109I GCAGC 1 cut(s) 1017
LweI GCATC 2 cut(s) 318, 447
MaeI CTAG 4 cut(s) 32, 461, 606, 836
MaeII ACGT 4 cut(s) 266, 359, 425, 927
MaeIII GTNAC 6 cut(s) 440, 794, 896, 928, 939, 959
MalI GATC 3 cut(s) 180, 492, 575
MboI GATC 3 cut(s) 178, 490, 573
MboII GAAGA 7 cut(s) 106, 129, 290, 448, 568, 739, 742
MhlI GDGCHC 3 cut(s) 706, 988, 1065
MluCI AATT 5 cut(s) 45, 60, 136, 256, 1072
MlyI GAGTC 4 cut(s) 320, 448, 518, 778
MmeI TCCRAC 1 cut(s) 301
MnlI CCTC 7 cut(s) 156, 393, 399, 430, 801, 942, 1028
Mph1103I ATGCAT 1 cut(s) 475
MseI TTAA 4 cut(s) 332, 537, 924, 1075
MslI CAYNNNNRTG 2 cut(s) 362, 777
MspA1I CMGCKG 1 cut(s) 946
MspCI CTTAAG 1 cut(s) 536
MspI CCGG 1 cut(s) 390
MspR9I CCNGG 1 cut(s) 1023
Mva1269I GAATGC 1 cut(s) 784
MvaI CCWGG 1 cut(s) 1023
MwoI GCNNNNNNNGC 5 cut(s) 470, 595, 943, 1002, 1014
NdeII GATC 3 cut(s) 178, 490, 573
NheI GCTAGC 1 cut(s) 460
NlaIII CATG 9 cut(s) 185, 255, 316, 367, 374, 555, 904, 1000, 1041
NlaIV GGNNCC 4 cut(s) 418, 599, 1016, 1043
NmuCI GTSAC 5 cut(s) 440, 896, 928, 939, 959
NsiI ATGCAT 1 cut(s) 475
NspI RCATGY 1 cut(s) 904
NspV TTCGAA 1 cut(s) 1069
PciI ACATGT 1 cut(s) 900
PcsI WCGNNNNNNNCGW 2 cut(s) 431, 528
PctI GAATGC 1 cut(s) 784
PfeI GAWTC 3 cut(s) 155, 367, 664
PkrI GCNGC 1 cut(s) 1007
PleI GAGTC 4 cut(s) 320, 447, 518, 777
PmaCI CACGTG 1 cut(s) 360
PmlI CACGTG 1 cut(s) 360
PpsI GAGTC 4 cut(s) 320, 447, 518, 777
Ppu21I YACGTR 1 cut(s) 360
PscI ACATGT 1 cut(s) 900
Psp124BI GAGCTC 1 cut(s) 1065
Psp1406I AACGTT 1 cut(s) 266
Psp6I CCWGG 1 cut(s) 1021
PspCI CACGTG 1 cut(s) 360
PspEI GGTNACC 2 cut(s) 794, 959
PspFI CCCAGC 1 cut(s) 865
PspGI CCWGG 1 cut(s) 1021
PspN4I GGNNCC 4 cut(s) 418, 599, 1016, 1043
PstI CTGCAG 1 cut(s) 1007
PstNI CAGNNNCTG 1 cut(s) 1051
PvuII CAGCTG 1 cut(s) 946
RsaI GTAC 1 cut(s) 1055
RsaNI GTAC 1 cut(s) 1054
RseI CAYNNNNRTG 2 cut(s) 362, 777
SacI GAGCTC 1 cut(s) 1065
SaqAI TTAA 4 cut(s) 332, 537, 924, 1075
SatI GCNGC 1 cut(s) 1006
Sau3AI GATC 3 cut(s) 178, 490, 573
SchI GAGTC 4 cut(s) 320, 448, 518, 778
ScrFI CCNGG 1 cut(s) 1023
SduI GDGCHC 3 cut(s) 706, 988, 1065
SfaNI GCATC 2 cut(s) 318, 447
SfcI CTRYAG 1 cut(s) 1003
SfuI TTCGAA 1 cut(s) 1069
SmiMI CAYNNNNRTG 2 cut(s) 362, 777
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
SpeI ACTAGT 1 cut(s) 835
Sse9I AATT 5 cut(s) 45, 60, 136, 256, 1072
SsiI CCGC 2 cut(s) 69, 414
SspMI CTAG 4 cut(s) 32, 461, 606, 836
SstI GAGCTC 1 cut(s) 1065
StyD4I CCNGG 1 cut(s) 1021
StyI CCWWGG 2 cut(s) 112, 991
TaaI ACNGT 2 cut(s) 621, 959
TaiI ACGT 4 cut(s) 269, 362, 428, 930
TaqI TCGA 9 cut(s) 189, 208, 235, 434, 522, 531, 655, 905, 1069
TasI AATT 5 cut(s) 45, 60, 136, 256, 1072
TatI WGTACW 1 cut(s) 1053
TfiI GAWTC 3 cut(s) 155, 367, 664
Tru1I TTAA 4 cut(s) 332, 537, 924, 1075
Tru9I TTAA 4 cut(s) 332, 537, 924, 1075
TscAI CASTG 1 cut(s) 1063
TseFI GTSAC 5 cut(s) 440, 896, 928, 939, 959
TseI GCWGC 1 cut(s) 1005
Tsp45I GTSAC 5 cut(s) 440, 896, 928, 939, 959
TspDTI ATGAA 3 cut(s) 240, 359, 677
TspRI CASTG 1 cut(s) 1063
Vha464I CTTAAG 1 cut(s) 536
XceI RCATGY 1 cut(s) 904
XspI CTAG 4 cut(s) 32, 461, 606, 836
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.