Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000567

Physical Location & Seq

Genomic Coordinates Forward (+)

9720692 .. 9723847

Gene Structure 3156 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1119700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1119 bp

ATGGCCCGCAGATCCGTGCAACTCCTCCGCCACCTCCTCCCGAAAGCAACCGCCCCGACCCTCTCCCCCACCTCCAACCCAACCCGATCCGTAACCTACATGCCCCGCCCCGGAGACGGCGCCCCTCGCGCCGTAACACTAATCCCCGGCGACGGCATCGGTCCTCTCGTTACCAACGCCGTCGAGCAGGTCATGGACGCGATGCATGCGCCAATCTACTTCGAGACCTTCGAGGTCCACGGCGACATGCCGAAGGTTCCGGATGAGGTGATGGAGTCGATCAAGAAGAACAAGGTGTGCTTGAAGGGCGGGCTGGCGACGCCCATGGGCGGCGGCGTGAGCTCTCTCAATTTGCAGCTGAGGAGGGAGCTTGATCTCTACGCCTCCCTCGTCAACTGCTTCAATCTGCCGGGGCTGGTGACGAAGCACGAGAATGTCGATATTGTCGTGATCAGGGAGAATACCGAGGGCGAGTACTCTGGGCTCGAACACGAGGTCATTCCTGGCGTCGTTGAAAGCCTTAAGGTGATCACAAAGTTCTGCTCGGAGCGTATTGCTAGATATGCGTTTGAGTATGCTTACCTAAACAACAGAAAGAAGGTGACTGCTGTGCACAAAGCAAACATTATGAAGCTTGCAGATGGTCTCTTCTTGGAGTCTTGTCGTGAGGTCGCCACAAACTATCCTAGCATTGCGTATAATGAAATCATTGTCGACAACTGCTGCATGCAACTTGTTTCGAAGCCCGAGCAATTTGATGTCATGGTGACACCGAATCTTTATGGCAATCTTGTTGCCAATACAGCAGCCGGTCTTGCTGGTGGCACTGGAGTTATGCCAGGAGGCAATGTTGGGGCTGATCATGCTATTTTCGAGCAAGGCGCTTCAGCAGGAAATGTTGGAAATCAGAGAATATTGGACCTGAAGAAAGCAAACCCAGTGGCCCTCCTTCTCTCCTCCGCCATGATGTTGAGACATCTGCAATTTCCGTCCTTTTCTGATCGACTAGAAACTGCAGTGAAACGAGTAATATTGGAGGGTAAATGCAGGACAAAGGACCTTGGAGGACAGAGCACAACCCAAGAGGTTGTTGACGCGGTCATTGCTGCCTTAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004448 GO:0004449 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006102 GO:0008150 GO:0008152 GO:0009060 GO:0009987 GO:0015980 GO:0016491 GO:0016614 GO:0016616 GO:0016999 GO:0017144 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0048046 GO:0055114 GO:0071704 GO:0072350

KEGG Pathways

Metabolic & Signaling

ko00020 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Iso_dh

Protein Analysis

373

Amino Acids

40.23

Weight (kDa)

6.47

Isoelectric Point (pI)

38.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Iso_dh	PF00180	45 - 367	2.2e-79	Isocitrate/isopropylmalate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000567)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35650
fragaria_vesca	FvH4_1g10550 FvH4_1g10550
malus_domestica	MD02G1119700.v1.1 MD15G1233500.v1.1
prunus_persica	Prupe.7G179400_v2.0.a1 Prupe.7G179400_v2.0.a1 Prupe.7G179400_v2.0.a1 Prupe.7G179400_v2.0.a1
pyrus_communis	pycom02g09320
rosa_chinensis	RchiOBHm_Chr0c39g0503111 RchiOBHm_Chr2g0097601 RchiOBHm_Chr2g0110731 RchiOBHm_Chr2g0135691 RchiOBHm_Chr2g0145421 RchiOBHm_Chr4g0395871 RchiOBHm_Chr4g0395881 RchiOBHm_Chr5g0074921 RchiOBHm_Chr6g0260871 RchiOBHm_Chr6g0268821
rosa_laevigata	RLG00000013432 RLG00000014392 RLG00000016727 RLG00000026056
rosa_multiflora	Rmu_co8326981.1_g000001 Rmu_sc0000104.1_g000032 Rmu_sc0002193.1_g000005 Rmu_sc0002271.1_g000027 Rmu_sc0004220.1_g000002 Rmu_sc0005347.1_g000007 Rmu_sc0006625.1_g000001 Rmu_sc0008835.1_g000001 Rmu_sc0009379.1_g000004
rosa_roxburghii	Rroxscaffold_2G00144670 Rroxscaffold_2G00153630
rosa_rugosa	Rorug01G0401400 Rorug02G0067700 Rorug02G0231100 Rorug03G0226800 Rorug03G0356000 Rorug04G0008700 Rorug05G0159200 Rorug05G0267100.1 Rorug05G0575000 Rorug05G0575000 Rorug06G0067200
rosa_samantha	Rh1BG010200 Rh1BG010300 Rh1BG252100 Rh1DG281100 Rh2AG114000 Rh2AG259300 Rh2AG567800 Rh2BG116800 Rh2BG383600 Rh2BG456400 Rh2CG118800 Rh2CG223900 Rh2DG117800 Rh2DG267700 Rh2DG466300 Rh3DG073900 Rh4AG245200 Rh4AG258800 Rh4BG250400 Rh4BG264600 Rh5AG127500 Rh5AG128100 Rh5AG506600 Rh5AG506700 Rh5AG507200 Rh5AG507300 Rh6AG184600 Rh6BG188000 Rh6CG192200 Rh6CG209400 Rh6DG437700 Rh6DG482100 Rh7AG364600 Rh7BG391200
rosa_wichuraiana	Rw2G008920 Rw3G027310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	178
AccB1I	GGYRCC	1 cut(s)	119
AccI	GTMKAC	1 cut(s)	714
AccII	CGCG	3 cut(s)	129, 200, 1097
AccIII	TCCGGA	1 cut(s)	259
AciI	CCGC	9 cut(s)	7, 28, 51, 106, 309, 330, 333, 960, 1097
AclWI	GGATC	2 cut(s)	6, 81
AcuI	CTGAAG	2 cut(s)	870, 944
AcyI	GRCGYC	3 cut(s)	120, 320, 507
AfaI	GTAC	1 cut(s)	476
AfiI	CCNNNNNNNGG	4 cut(s)	110, 116, 152, 329
AflII	CTTAAG	1 cut(s)	521
AgsI	TTSAA	3 cut(s)	304, 403, 515
AjnI	CCWGG	2 cut(s)	502, 838
AluBI	AGCT	4 cut(s)	342, 358, 370, 634
AluI	AGCT	4 cut(s)	342, 358, 370, 634
Alw21I	GWGCWC	3 cut(s)	344, 615, 1076
Alw26I	GTCTC	4 cut(s)	108, 218, 650, 967
Alw44I	GTGCAC	1 cut(s)	611
AlwI	GGATC	2 cut(s)	6, 81
Ama87I	CYCGRG	1 cut(s)	746
Aor13HI	TCCGGA	1 cut(s)	259
AoxI	GGCC	2 cut(s)	3, 942
ApaLI	GTGCAC	1 cut(s)	611
ApeKI	GCWGC	4 cut(s)	355, 723, 806, 1106
ArsI	GACNNNNNNTTYG	2 cut(s)	480, 512
AspLEI	GCGC	4 cut(s)	122, 131, 211, 884
AspS9I	GGNCC	6 cut(s)	4, 161, 235, 919, 943, 1057
AsuC2I	CCSGG	3 cut(s)	111, 147, 411
AsuHPI	GGTGA	5 cut(s)	280, 430, 538, 613, 778
AsuII	TTCGAA	1 cut(s)	740
AvaI	CYCGRG	1 cut(s)	746
AvaII	GGWCC	4 cut(s)	161, 235, 919, 1057
BaeGI	GKGCMC	1 cut(s)	615
BanI	GGYRCC	1 cut(s)	119
BanII	GRGCYC	2 cut(s)	344, 486
BauI	CACGAG	2 cut(s)	428, 491
Bbv12I	GWGCWC	3 cut(s)	344, 615, 1076
BbvCI	CCTCAGC	1 cut(s)	359
BbvI	GCAGC	4 cut(s)	367, 710, 818, 1093
BccI	CCATC	2 cut(s)	265, 635
BceAI	ACGGC	5 cut(s)	116, 133, 164, 169, 256
BciT130I	CCWGG	2 cut(s)	504, 840
BclI	TGATCA	3 cut(s)	450, 528, 859
BcnI	CCSGG	3 cut(s)	111, 147, 411
BcoDI	GTCTC	4 cut(s)	108, 218, 650, 967
BfaI	CTAG	3 cut(s)	558, 687, 1007
BfmI	CTRYAG	1 cut(s)	1014
BfoI	RGCGCY	2 cut(s)	123, 885
BfrI	CTTAAG	1 cut(s)	521
BfuAI	ACCTGC	1 cut(s)	178
BisI	GCNGC	6 cut(s)	331, 334, 356, 724, 807, 1107
BlsI	GCNGC	6 cut(s)	332, 335, 357, 725, 808, 1108
BmcAI	AGTACT	1 cut(s)	476
Bme1390I	CCNGG	5 cut(s)	111, 147, 411, 504, 840
Bme18I	GGWCC	4 cut(s)	161, 235, 919, 1057
BmeT110I	CYCGRG	1 cut(s)	746
BmgT120I	GGNCC	6 cut(s)	4, 161, 235, 919, 943, 1057
BmiI	GGNNCC	2 cut(s)	121, 258
BmrFI	CCNGG	5 cut(s)	111, 147, 411, 504, 840
BmrI	ACTGGG	1 cut(s)	932
BmsI	GCATC	2 cut(s)	165, 192
BmuI	ACTGGG	1 cut(s)	932
BpmI	CTGGAG	1 cut(s)	849
Bpu10I	CCTNAGC	1 cut(s)	359
Bpu14I	TTCGAA	1 cut(s)	740
BpuMI	CCSGG	3 cut(s)	111, 147, 411
BsaHI	GRCGYC	3 cut(s)	120, 320, 507
BsaI	GGTCTC	2 cut(s)	218, 650
BsaJI	CCNNGG	7 cut(s)	109, 145, 238, 324, 410, 465, 1060
BsaWI	WCCGGW	1 cut(s)	259
BsaXI	ACNNNNNCTCC	2 cut(s)	834, 864
Bsc4I	CCNNNNNNNGG	4 cut(s)	110, 116, 152, 329
Bse118I	RCCGGY	1 cut(s)	809
Bse1I	ACTGG	2 cut(s)	832, 938
Bse3DI	GCAATG	3 cut(s)	690, 853, 1101
BseAI	TCCGGA	1 cut(s)	259
BseBI	CCWGG	2 cut(s)	504, 840
BseDI	CCNNGG	7 cut(s)	109, 145, 238, 324, 410, 465, 1060
BseGI	GGATG	1 cut(s)	268
BseLI	CCNNNNNNNGG	4 cut(s)	110, 116, 152, 329
BseMI	GCAATG	3 cut(s)	690, 853, 1101
BseMII	CTCAG	1 cut(s)	350
BseNI	ACTGG	2 cut(s)	832, 938
BseRI	GAGGAG	4 cut(s)	14, 26, 376, 946
BseSI	GKGCMC	1 cut(s)	615
BseXI	GCAGC	4 cut(s)	367, 710, 818, 1093
Bsh1236I	CGCG	3 cut(s)	129, 200, 1097
BshFI	GGCC	2 cut(s)	5, 944
BshNI	GGYRCC	1 cut(s)	119
BsiHKAI	GWGCWC	3 cut(s)	344, 615, 1076
BsiHKCI	CYCGRG	1 cut(s)	746
BsiSI	CCGG	5 cut(s)	111, 147, 260, 410, 810
BslI	CCNNNNNNNGG	4 cut(s)	110, 116, 152, 329
BsmAI	GTCTC	4 cut(s)	108, 218, 650, 967
BsmBI	CGTCTC	1 cut(s)	108
BsnI	GGCC	2 cut(s)	5, 944
Bso31I	GGTCTC	2 cut(s)	218, 650
BsoBI	CYCGRG	1 cut(s)	746
Bsp119I	TTCGAA	1 cut(s)	740
Bsp1286I	GDGCHC	4 cut(s)	344, 486, 615, 1076
Bsp13I	TCCGGA	1 cut(s)	259
Bsp143I	GATC	8 cut(s)	11, 86, 279, 373, 450, 528, 859, 1000
Bsp19I	CCATGG	1 cut(s)	324
BspACI	CCGC	9 cut(s)	7, 28, 51, 106, 309, 330, 333, 960, 1097
BspANI	GGCC	2 cut(s)	5, 944
BspCNI	CTCAG	1 cut(s)	351
BspEI	TCCGGA	1 cut(s)	259
BspFNI	CGCG	3 cut(s)	129, 200, 1097
BspLI	GGNNCC	2 cut(s)	121, 258
BspMAI	CTGCAG	1 cut(s)	1018
BspMI	ACCTGC	1 cut(s)	178
BspPI	GGATC	2 cut(s)	6, 81
BspT104I	TTCGAA	1 cut(s)	740
BspT107I	GGYRCC	1 cut(s)	119
BspTI	CTTAAG	1 cut(s)	521
BspTNI	GGTCTC	2 cut(s)	218, 650
BsrDI	GCAATG	3 cut(s)	690, 853, 1101
BsrFI	RCCGGY	1 cut(s)	809
BsrI	ACTGG	2 cut(s)	832, 938
BssAI	RCCGGY	1 cut(s)	809
BssECI	CCNNGG	7 cut(s)	109, 145, 238, 324, 410, 465, 1060
BssMI	GATC	8 cut(s)	11, 86, 279, 373, 450, 528, 859, 1000
BssNI	GRCGYC	3 cut(s)	120, 320, 507
BssSI	CACGAG	2 cut(s)	428, 491
BssT1I	CCWWGG	2 cut(s)	324, 1060
Bst2BI	CACGAG	2 cut(s)	428, 491
Bst2UI	CCWGG	2 cut(s)	504, 840
Bst6I	CTCTTC	1 cut(s)	653
BstACI	GRCGYC	3 cut(s)	120, 320, 507
BstAFI	CTTAAG	1 cut(s)	521
BstBI	TTCGAA	1 cut(s)	740
BstC8I	GCNNGC	6 cut(s)	7, 207, 311, 315, 636, 728
BstDEI	CTNAG	2 cut(s)	359, 1111
BstDSI	CCRYGG	2 cut(s)	238, 324
BstF5I	GGATG	1 cut(s)	268
BstFNI	CGCG	3 cut(s)	129, 200, 1097
BstH2I	RGCGCY	2 cut(s)	123, 885
BstHHI	GCGC	4 cut(s)	122, 131, 211, 884
BstKTI	GATC	8 cut(s)	14, 89, 282, 376, 453, 531, 862, 1003
BstMAI	GTCTC	4 cut(s)	108, 218, 650, 967
BstMBI	GATC	8 cut(s)	11, 86, 279, 373, 450, 528, 859, 1000
BstNI	CCWGG	2 cut(s)	504, 840
BstNSI	RCATGY	4 cut(s)	103, 209, 250, 730
BstSCI	CCNGG	5 cut(s)	109, 145, 409, 502, 838
BstSFI	CTRYAG	1 cut(s)	1014
BstSLI	GKGCMC	1 cut(s)	615
BstUI	CGCG	3 cut(s)	129, 200, 1097
BstV1I	GCAGC	4 cut(s)	367, 710, 818, 1093
BstX2I	RGATCY	1 cut(s)	11
BstYI	RGATCY	1 cut(s)	11
BsuRI	GGCC	2 cut(s)	5, 944
BtgI	CCRYGG	2 cut(s)	238, 324
BtgZI	GCGATG	1 cut(s)	215
BtsCI	GGATG	1 cut(s)	268
BtsI	GCAGTG	1 cut(s)	1023
BtsIMutI	CAGTG	3 cut(s)	825, 945, 1023
BveI	ACCTGC	1 cut(s)	178
Cac8I	GCNNGC	6 cut(s)	7, 207, 311, 315, 636, 728
CfoI	GCGC	4 cut(s)	122, 131, 211, 884
Cfr10I	RCCGGY	1 cut(s)	809
Cfr13I	GGNCC	6 cut(s)	4, 161, 235, 919, 943, 1057
CseI	GACGC	4 cut(s)	206, 328, 496, 1103
Csp6I	GTAC	1 cut(s)	475
CspCI	CAANNNNNGTGG	2 cut(s)	921, 956
CviAII	CATG	9 cut(s)	100, 193, 206, 247, 325, 727, 763, 863, 964
CviQI	GTAC	1 cut(s)	475
DdeI	CTNAG	2 cut(s)	359, 1111
DinI	GGCGCC	1 cut(s)	121
DpnI	GATC	8 cut(s)	13, 88, 281, 375, 452, 530, 861, 1002
DpnII	GATC	8 cut(s)	11, 86, 279, 373, 450, 528, 859, 1000
Eam1104I	CTCTTC	1 cut(s)	653
EarI	CTCTTC	1 cut(s)	653
EciI	GGCGGA	2 cut(s)	17, 949
Ecl136II	GAGCTC	1 cut(s)	342
Eco130I	CCWWGG	2 cut(s)	324, 1060
Eco24I	GRGCYC	2 cut(s)	344, 486
Eco31I	GGTCTC	2 cut(s)	218, 650
Eco47I	GGWCC	4 cut(s)	161, 235, 919, 1057
Eco53kI	GAGCTC	1 cut(s)	342
Eco57I	CTGAAG	2 cut(s)	870, 944
Eco88I	CYCGRG	1 cut(s)	746
EcoICRI	GAGCTC	1 cut(s)	342
EcoO109I	RGGNCCY	1 cut(s)	1057
EcoRII	CCWGG	2 cut(s)	502, 838
EcoT14I	CCWWGG	2 cut(s)	324, 1060
EcoT22I	ATGCAT	1 cut(s)	207
EcoT38I	GRGCYC	2 cut(s)	344, 486
EgeI	GGCGCC	1 cut(s)	121
EheI	GGCGCC	1 cut(s)	121
ErhI	CCWWGG	2 cut(s)	324, 1060
Esp3I	CGTCTC	1 cut(s)	108
FaeI	CATG	9 cut(s)	103, 196, 209, 250, 328, 730, 766, 866, 967
FalI	AAGNNNNNCTT	2 cut(s)	284, 316
FatI	CATG	9 cut(s)	99, 192, 205, 246, 324, 726, 762, 862, 963
FauI	CCCGC	3 cut(s)	14, 113, 302
FbaI	TGATCA	3 cut(s)	450, 528, 859
FblI	GTMKAC	1 cut(s)	714
Fnu4HI	GCNGC	6 cut(s)	331, 334, 356, 724, 807, 1107
FokI	GGATG	1 cut(s)	275
FriOI	GRGCYC	2 cut(s)	344, 486
Fsp4HI	GCNGC	6 cut(s)	331, 334, 356, 724, 807, 1107
FspBI	CTAG	3 cut(s)	558, 687, 1007
GlaI	GCGC	4 cut(s)	121, 130, 210, 883
GluI	GCNGC	6 cut(s)	331, 334, 356, 724, 807, 1107
GsuI	CTGGAG	1 cut(s)	849
HaeII	RGCGCY	2 cut(s)	123, 885
HaeIII	GGCC	2 cut(s)	5, 944
HapII	CCGG	5 cut(s)	111, 147, 260, 410, 810
HgaI	GACGC	4 cut(s)	206, 328, 496, 1103
HhaI	GCGC	4 cut(s)	122, 131, 211, 884
Hin1I	GRCGYC	3 cut(s)	120, 320, 507
Hin1II	CATG	9 cut(s)	103, 196, 209, 250, 328, 730, 766, 866, 967
Hin6I	GCGC	4 cut(s)	120, 129, 209, 882
HinP1I	GCGC	4 cut(s)	120, 129, 209, 882
HincII	GTYRAC	3 cut(s)	394, 715, 1093
HindII	GTYRAC	3 cut(s)	394, 715, 1093
HindIII	AAGCTT	1 cut(s)	632
HinfI	GANTC	3 cut(s)	275, 656, 775
HpaII	CCGG	5 cut(s)	111, 147, 260, 410, 810
HphI	GGTGA	5 cut(s)	280, 430, 538, 613, 778
Hpy166II	GTNNAC	5 cut(s)	238, 394, 613, 715, 1093
Hpy188I	TCNGA	3 cut(s)	547, 909, 1000
Hpy188III	TCNNGA	6 cut(s)	40, 223, 260, 283, 448, 665
Hpy8I	GTNNAC	5 cut(s)	238, 394, 613, 715, 1093
Hpy99I	CGWCG	4 cut(s)	155, 185, 322, 512
HpyAV	CCTTC	5 cut(s)	238, 247, 298, 592, 959
HpyF3I	CTNAG	2 cut(s)	359, 1111
Hsp92I	GRCGYC	3 cut(s)	120, 320, 507
Hsp92II	CATG	9 cut(s)	103, 196, 209, 250, 328, 730, 766, 866, 967
HspAI	GCGC	4 cut(s)	120, 129, 209, 882
KasI	GGCGCC	1 cut(s)	119
Kpn2I	TCCGGA	1 cut(s)	259
Ksp22I	TGATCA	3 cut(s)	450, 528, 859
Kzo9I	GATC	8 cut(s)	11, 86, 279, 373, 450, 528, 859, 1000
LmnI	GCTCC	2 cut(s)	367, 547
Lsp1109I	GCAGC	4 cut(s)	367, 710, 818, 1093
LweI	GCATC	2 cut(s)	165, 192
MaeI	CTAG	3 cut(s)	558, 687, 1007
MaeIII	GTNAC	6 cut(s)	91, 133, 169, 418, 601, 766
MalI	GATC	8 cut(s)	13, 88, 281, 375, 452, 530, 861, 1002
MboI	GATC	8 cut(s)	11, 86, 279, 373, 450, 528, 859, 1000
MboII	GAAGA	3 cut(s)	298, 640, 937
MflI	RGATCY	1 cut(s)	11
MhlI	GDGCHC	4 cut(s)	344, 486, 615, 1076
MluCI	AATT	3 cut(s)	349, 752, 983
Mly113I	GGCGCC	1 cut(s)	120
MlyI	GAGTC	2 cut(s)	284, 665
MmeI	TCCRAC	2 cut(s)	99, 880
Mph1103I	ATGCAT	1 cut(s)	207
MroI	TCCGGA	1 cut(s)	259
MseI	TTAA	1 cut(s)	522
MslI	CAYNNNNRTG	1 cut(s)	432
MspA1I	CMGCKG	1 cut(s)	358
MspCI	CTTAAG	1 cut(s)	521
MspI	CCGG	5 cut(s)	111, 147, 260, 410, 810
MspR9I	CCNGG	5 cut(s)	111, 147, 411, 504, 840
MvaI	CCWGG	2 cut(s)	504, 840
MvnI	CGCG	3 cut(s)	129, 200, 1097
NarI	GGCGCC	1 cut(s)	120
NciI	CCSGG	3 cut(s)	111, 147, 411
NcoI	CCATGG	1 cut(s)	324
NdeII	GATC	8 cut(s)	11, 86, 279, 373, 450, 528, 859, 1000
NlaIII	CATG	9 cut(s)	103, 196, 209, 250, 328, 730, 766, 866, 967
NlaIV	GGNNCC	2 cut(s)	121, 258
NmuCI	GTSAC	3 cut(s)	418, 601, 766
NsiI	ATGCAT	1 cut(s)	207
NspI	RCATGY	4 cut(s)	103, 209, 250, 730
NspV	TTCGAA	1 cut(s)	740
PaeI	GCATGC	2 cut(s)	209, 730
PcsI	WCGNNNNNNNCGW	5 cut(s)	165, 228, 387, 435, 444
PfeI	GAWTC	1 cut(s)	775
PflFI	GACNNNGTC	1 cut(s)	1097
PkrI	GCNGC	6 cut(s)	332, 335, 357, 725, 808, 1108
PleI	GAGTC	2 cut(s)	283, 664
PluTI	GGCGCC	1 cut(s)	123
PpsI	GAGTC	2 cut(s)	283, 664
PpuMI	RGGWCCY	1 cut(s)	1057
Psp124BI	GAGCTC	1 cut(s)	344
Psp5II	RGGWCCY	1 cut(s)	1057
Psp6I	CCWGG	2 cut(s)	502, 838
PspGI	CCWGG	2 cut(s)	502, 838
PspN4I	GGNNCC	2 cut(s)	121, 258
PspPI	GGNCC	6 cut(s)	4, 161, 235, 919, 943, 1057
PspPPI	RGGWCCY	1 cut(s)	1057
PstI	CTGCAG	1 cut(s)	1018
PsuI	RGATCY	1 cut(s)	11
PsyI	GACNNNGTC	1 cut(s)	1097
PvuII	CAGCTG	1 cut(s)	358
RsaI	GTAC	1 cut(s)	476
RsaNI	GTAC	1 cut(s)	475
RseI	CAYNNNNRTG	1 cut(s)	432
SacI	GAGCTC	1 cut(s)	344
SalI	GTCGAC	1 cut(s)	713
SaqAI	TTAA	1 cut(s)	522
SatI	GCNGC	6 cut(s)	331, 334, 356, 724, 807, 1107
Sau3AI	GATC	8 cut(s)	11, 86, 279, 373, 450, 528, 859, 1000
Sau96I	GGNCC	6 cut(s)	4, 161, 235, 919, 943, 1057
ScaI	AGTACT	1 cut(s)	476
SchI	GAGTC	2 cut(s)	284, 665
ScrFI	CCNGG	5 cut(s)	111, 147, 411, 504, 840
SduI	GDGCHC	4 cut(s)	344, 486, 615, 1076
SfaNI	GCATC	2 cut(s)	165, 192
SfcI	CTRYAG	1 cut(s)	1014
SfoI	GGCGCC	1 cut(s)	121
SfuI	TTCGAA	1 cut(s)	740
SinI	GGWCC	4 cut(s)	161, 235, 919, 1057
SmiMI	CAYNNNNRTG	1 cut(s)	432
SmlI	CTYRAG	1 cut(s)	521
SmoI	CTYRAG	1 cut(s)	521
SphI	GCATGC	2 cut(s)	209, 730
Sse9I	AATT	3 cut(s)	349, 752, 983
SsiI	CCGC	9 cut(s)	7, 28, 51, 106, 309, 330, 333, 960, 1097
SspDI	GGCGCC	1 cut(s)	119
SspI	AATATT	2 cut(s)	915, 1032
SspMI	CTAG	3 cut(s)	558, 687, 1007
SstI	GAGCTC	1 cut(s)	344
StyD4I	CCNGG	5 cut(s)	109, 145, 409, 502, 838
StyI	CCWWGG	2 cut(s)	324, 1060
TaqII	GACCGA	1 cut(s)	149
TasI	AATT	3 cut(s)	349, 752, 983
TatI	WGTACW	1 cut(s)	474
TauI	GCSGC	2 cut(s)	333, 336
TfiI	GAWTC	1 cut(s)	775
Tru1I	TTAA	1 cut(s)	522
Tru9I	TTAA	1 cut(s)	522
TscAI	CASTG	3 cut(s)	832, 945, 1023
TseFI	GTSAC	3 cut(s)	418, 601, 766
TseI	GCWGC	4 cut(s)	355, 723, 806, 1106
Tsp45I	GTSAC	3 cut(s)	418, 601, 766
TspDTI	ATGAA	2 cut(s)	644, 717
TspGWI	ACGGA	3 cut(s)	4, 79, 978
TspRI	CASTG	3 cut(s)	832, 945, 1023
Tth111I	GACNNNGTC	1 cut(s)	1097
Vha464I	CTTAAG	1 cut(s)	521
VneI	GTGCAC	1 cut(s)	611
VpaK11BI	GGWCC	4 cut(s)	161, 235, 919, 1057
XceI	RCATGY	4 cut(s)	103, 209, 250, 730
XmiI	GTMKAC	1 cut(s)	714
XspI	CTAG	3 cut(s)	558, 687, 1007
ZrmI	AGTACT	1 cut(s)	476
Zsp2I	ATGCAT	1 cut(s)	207

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.