Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000855

Physical Location & Seq

Genomic Coordinates Reverse (-)

10008280 .. 10013911

Gene Structure 5632 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1123100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2583 bp

ATGGCCGAAACGGAGTCGGAGGAGCGGATGATCTACCTGCACGGCGACCTCGACCTCACAATCTTCGAAGCCCGCCGCCTCCCCAACATGGACATGTTCTCCGAGCGCTTCCGCCGCTGCTTCACCGCCTGCGACACCATCAATATCTCCTCCACCCCCTCCGCCGAAGACGACGGCGGTCACGACGGTGGAGAACGCAAGAACCACGTTCCCCACCGAAAGATCATCACCAGCGATTCATACGTCACCGTTACGGTCCCCCAGACCACCGTCGCCCGGACACGTGTCATGAAGAACTCCCAGAACCCGCAGTGGAAGGAGCACTTCTTTGTCCCGCTGGCGCACCCTGCAATTAACCTCGAGTTTCACGTCAAAGATGACGACGTCTTTGGCGCTGAGACCATCGGAACCGCCGAGATCCCGGCGGAGAGAATCGCCACCGGAGAGCTCATCTCCGGGTGGTTCCCCATTTACGGACCCTCCGGGAAGCCCCCGAAACCCGACTCGGCGATCAAGCTCGAATTGCAGTTCACGCCGTTCGAGAAAAACCCAGTGTACAAGCAAGGCATAGCGGGGGATCCGGAGCACAAGGGCGTGCAGAACACGTACTTCCCGCTCCGGAAGGGAAGCTCCGTGAGGTTGTACCAAGACGCGCACTGCCCTGCAGGGCTGCTGCCGGAGATACAGTTGGACGGTGGGAAGGTGTACAAGGCGGAGAATACATGGGAGGACATTTGCTATGCGATTACGGAAGCGCACCACTTGGTTTACATTGTGGGTTGGTCGGTGTTTCACAAGGTGCGGCTGATTCGGGAGCCGAGCCGGCCGCTGCCGCGCGGCGGCGACCTGTTGCTCGGCGATTTGCTCAAGTACAAGTCGGAGGAGGGCGTGAGGGTGTTGCTGTTGGTGTGGGACGATAAGTCCTCTCACGACAAGTTCGGCTTCAAAACGGCAGGAATGATGGGGACACATGATGAAGAAACCCGGAAGTTTTTCAAGCATTCATCTGTAAATTGTGTGCTGTCAACTCGCTACGCTAGCAGTAAGCTTAGCATTATAAAACAACAGGTTGTTGGAACCCTTTTCACCCACCATCAAAAATGTGTTCTTGTGGACACACAGGCTGACGGTAATAATCGGAAGATAACTGCATTTTTAGGAGGTCTTGATCTTTGTGATGGTCGTTATGACACACCCGAGCACAGACTGTTTCGTAATCTTGACACTGTATTTAAGGGTGATGTTCATCAACCTACTTTTCCTGCTGGAACCAAGGCTCCAAGACAACCATGGCATGACTTGCACTGTAGAGTTGATGGGCCTGCTGCATATGATGTTCTTATAAACTTTGAGCAACGTTGGAGGAAAGCAACGCGGTGGAAGGAGTTTGCACTACTGAGTAAGAAGGTTTCACATTGGCATGACGATGCATTGATAAAAATTGACCGCATCTCCTGGATACTAAGTCCTCCCCTATCTGTTTCAAAGGATGGTACAACTATTCCAGAAGACGAGCCTTTGTTGTGGGTTAACAATGAAAAAGATCCCGAAAATTGGCATGTTCAGATTTTCCGGTCCATTGACTCAGGATCACTGAAAGGATTTCCCAAAGCTGGCCGTGCTGCTGAGGCCAAGCATCTTATTTGCTCAAAGAATCTGGTAATAGATAAAAGCATTCAAACAGCTTACATCCAGGCAATCAGATCCGCCCAACACTTTATATATATCGAAAATCAGTATTTTCTTGGTTCATCATATGCATGGCCAGATTATAAAAATGCAGGAGCTGATAATCTTATCCCAATGGAGTTGGCATTAAAAATTGTTAGCAAAATTAAAGCTAATGAGAGATTCACGGTGTATGTCGTCTTACCTATGTGGCCTGAAGGTGATCCAAAAACTCTCGCAATGCAAGAAATTCTCTTCTGGCAGAGGCAGACAATGCAAGCAATGTATAGTACTGTTGCAGCGGCACTGAAATCGGTGCAGATTCAGGACTCACATCCTCAAGATTACCTAAGTTTCTATTGCCTTGGTAACCGGGAAATACTTCCTGAGGATACTGCAAATGACGATGCGTCTTCGGTTTCCGATTCACAAAAAAATCAGCGGTTTATGATTTATGTTCATGCCAAGGGTATGGTAGTAGATGACGAGTATGTAATATTGGGATCTGCCAATATCAACCAAAGATCAATGGCTGGTACAAAGGACACTGAGATAGCCATGGGTGCGTATCAACCCCATTACACATGGGCTGCGAGGAAGAAACATCCGCGTGGTCAGATATATGGATATCGAATGTCACTTTGGGCCGAGCATCTTGATATGTTGGATCCATGCTTCGAAGAGCCTGAGAGTTTGAAATGTATGAGGAAAGTAAATGAGGTTGCAGGCGAGAACTGGACGAGATTCACATCTCCAGAGTTTACACTACTGCAGGGTCACCTTATGAAGTATCCTATTGACGTAGATGTTGATGGGAAGGTCGGCCCCCTGCCGGGACATGAAAACTTCCCTGATGTTGGTGGTAAGATACTTGGATCTCATTCCAACAAAATTCCTGATATGCTCACGACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006950 GO:0008081 GO:0008150 GO:0008152 GO:0008219 GO:0009266 GO:0009409 GO:0009506 GO:0009628 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010119 GO:0010646 GO:0010647 GO:0012501 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019637 GO:0023051 GO:0023056 GO:0030054 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046473 GO:0046486 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0090333 GO:1901419 GO:1901421 GO:1905957 GO:1905959

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

861

Amino Acids

97.49

Weight (kDa)

6.4

Isoelectric Point (pI)

33.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	75 - 156	1.7e-15	C2 domain
PLDc_2	PF13091	706 - 745	8.1e-06	PLD-like domain
PLDc	PF00614	707 - 732	1e-07	Phospholipase D Active site motif
PLD_C	PF12357	780 - 849	2.5e-29	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000855)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35790 AT4G35790 AT4G35790
fragaria_vesca	FvH4_1g11000 FvH4_1g11020 FvH4_1g11020 FvH4_2g37900 FvH4_5g01272 FvH4_5g28780 FvH4_5g28800 FvH4_7g08510 FvH4_7g08510
malus_domestica	MD02G1123100.v1.1 MD08G1149700.v1.1 MD08G1237200.v1.1 MD15G1006400.v1.1 MD15G1237100.v1.1
prunus_persica	Prupe.1G359000_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.7G175400_v2.0.a1
pyrus_communis	pycom02g09600 pycom15g00480 pycom15g21090
rosa_chinensis	RchiOBHm_Chr2g0098271 RchiOBHm_Chr6g0303711 RchiOBHm_Chr7g0221001 RchiOBHm_Chr7g0221011
rosa_laevigata	RLG00000002185 RLG00000011053 RLG00000016781
rosa_multiflora	Rmu_co8414259.1_g000001 Rmu_co8436567.1_g000002 Rmu_co8452585.1_g000001 Rmu_sc0001024.1_g000003 Rmu_sc0002105.1_g000010 Rmu_sc0002105.1_g000018 Rmu_sc0002105.1_g000020 Rmu_sc0009922.1_g000005 Rmu_sc0039265.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144090 Rroxscaffold_3G00238820 Rroxscaffold_7G00164460
rosa_rugosa	Rorug02G0071800 Rorug06G0324200 Rorug07G0199200 Rorug07G0199300 Rorug07G0199400 Rorug07G0199500 Rorug07G0199600
rosa_samantha	Rh1CG003000 Rh2AG118900 Rh2BG121800 Rh2CG123400 Rh2DG123500 Rh6AG438500 Rh6BG464500 Rh6CG450800 Rh6DG437500 Rh7AG339200 Rh7BG329900 Rh7CG357200 Rh7DG337900
rosa_wichuraiana	Rw2G009320 Rw6G037950 Rw7G028850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1058, 1343, 1773
AatII	GACGTC	1 cut(s)	387
Acc36I	ACCTGC	1 cut(s)	45
AccBSI	CCGCTC	2 cut(s)	25, 616
AccII	CGCG	5 cut(s)	653, 835, 837, 1375, 2278
AccIII	TCCGGA	2 cut(s)	580, 618
AclI	AACGTT	1 cut(s)	1357
AcoI	YGGCCR	4 cut(s)	3, 824, 1615, 1763
AcsI	RAATTY	2 cut(s)	1917, 2559
AcuI	CTGAAG	1 cut(s)	1905
AcvI	CACGTG	1 cut(s)	284
AcyI	GRCGYC	1 cut(s)	384
AdeI	CACNNNGTG	1 cut(s)	799
AfaI	GTAC	8 cut(s)	557, 608, 644, 707, 872, 1495, 1960, 2206
AfeI	AGCGCT	1 cut(s)	107
AfiI	CCNNNNNNNGG	9 cut(s)	88, 276, 473, 839, 1554, 1613, 2500, 2501, 2525
AflIII	ACRYGT	4 cut(s)	93, 281, 283, 603
AgsI	TTSAA	5 cut(s)	946, 997, 1485, 1679, 2365
AhdI	GACNNNNNGTC	2 cut(s)	919, 2077
AjiI	CACGTC	1 cut(s)	370
AjnI	CCWGG	2 cut(s)	1454, 1692
AjuI	GAANNNNNNNTTGG	2 cut(s)	2293, 2325
AleI	CACNNNNGTG	1 cut(s)	186
AloI	GAACNNNNNNTCC	2 cut(s)	1261, 1293
AluBI	AGCT	8 cut(s)	448, 517, 630, 1048, 1613, 1685, 1787, 1841
AluI	AGCT	8 cut(s)	448, 517, 630, 1048, 1613, 1685, 1787, 1841
Alw21I	GWGCWC	4 cut(s)	324, 450, 588, 1203
Alw26I	GTCTC	1 cut(s)	392
AlwNI	CAGNNNCTG	1 cut(s)	1787
Ama87I	CYCGRG	2 cut(s)	359, 1196
Aor13HI	TCCGGA	2 cut(s)	580, 618
Aor51HI	AGCGCT	1 cut(s)	107
AoxI	GGCC	9 cut(s)	3, 824, 1319, 1615, 1629, 1763, 1880, 2313, 2491
ApeKI	GCWGC	8 cut(s)	117, 670, 673, 829, 1325, 1622, 1967, 2258
ApoI	RAATTY	2 cut(s)	1917, 2559
ArsI	GACNNNNNNTTYG	2 cut(s)	371, 403
Asp700I	GAANNNNTTC	2 cut(s)	992, 2049
AspLEI	GCGC	6 cut(s)	108, 343, 395, 655, 757, 837
AspS9I	GGNCC	6 cut(s)	256, 476, 1319, 1575, 2313, 2492
AsuC2I	CCSGG	7 cut(s)	277, 422, 457, 484, 985, 2042, 2502
AsuHPI	GGTGA	7 cut(s)	115, 220, 238, 1078, 1250, 1901, 2438
AsuII	TTCGAA	2 cut(s)	66, 2346
AsuNHI	GCTAGC	1 cut(s)	1037
AvaI	CYCGRG	2 cut(s)	359, 1196
AvaII	GGWCC	3 cut(s)	256, 476, 1575
AxyI	CCTNAGG	1 cut(s)	2055
BalI	TGGCCA	1 cut(s)	1765
BamHI	GGATCC	2 cut(s)	577, 2335
BanII	GRGCYC	1 cut(s)	450
BbrPI	CACGTG	1 cut(s)	284
BbsI	GAAGAC	3 cut(s)	174, 1515, 2073
Bbv12I	GWGCWC	4 cut(s)	324, 450, 588, 1203
BbvCI	CCTCAGC	1 cut(s)	1626
BbvI	GCAGC	8 cut(s)	104, 657, 660, 816, 1312, 1609, 1979, 2245
BccI	CCATC	8 cut(s)	146, 410, 955, 1101, 1172, 1310, 1484, 2474
BceAI	ACGGC	5 cut(s)	58, 190, 520, 966, 1602
BcgI	CGANNNNNNTGC	2 cut(s)	1962, 1996
BciT130I	CCWGG	2 cut(s)	1456, 1694
BciVI	GTATCC	3 cut(s)	1452, 2053, 2469
BcnI	CCSGG	7 cut(s)	277, 422, 457, 484, 985, 2042, 2502
BcoDI	GTCTC	1 cut(s)	392
BfaI	CTAG	1 cut(s)	1038
BfmI	CTRYAG	3 cut(s)	663, 1306, 2438
BfoI	RGCGCY	2 cut(s)	109, 396
BfuAI	ACCTGC	1 cut(s)	45
BfuI	GTATCC	3 cut(s)	1452, 2053, 2469
BglI	GCCNNNNNGGC	1 cut(s)	823
BlpI	GCTNAGC	1 cut(s)	1049
BmcAI	AGTACT	1 cut(s)	1960
Bme1390I	CCNGG	9 cut(s)	277, 422, 457, 484, 985, 1456, 1694, 2042, 2502
Bme18I	GGWCC	3 cut(s)	256, 476, 1575
BmeRI	GACNNNNNGTC	2 cut(s)	919, 2077
BmeT110I	CYCGRG	2 cut(s)	359, 1196
BmgBI	CACGTC	1 cut(s)	370
BmgT120I	GGNCC	6 cut(s)	256, 476, 1319, 1575, 2313, 2492
BmrFI	CCNGG	9 cut(s)	277, 422, 457, 484, 985, 1456, 1694, 2042, 2502
BmrI	ACTGGG	1 cut(s)	545
BmsI	GCATC	5 cut(s)	1417, 1458, 1645, 2065, 2329
BmtI	GCTAGC	1 cut(s)	1041
BmuI	ACTGGG	1 cut(s)	545
BoxI	GACNNNNGTC	3 cut(s)	177, 269, 284
BpiI	GAAGAC	3 cut(s)	174, 1515, 2073
BplI	GAGNNNNNCTC	2 cut(s)	437, 469
BpmI	CTGGAG	1 cut(s)	2406
Bpu10I	CCTNAGC	1 cut(s)	1626
Bpu1102I	GCTNAGC	1 cut(s)	1049
Bpu14I	TTCGAA	2 cut(s)	66, 2346
BpuEI	CTTGAG	2 cut(s)	851, 1992
BpuMI	CCSGG	7 cut(s)	277, 422, 457, 484, 985, 2042, 2502
BsaAI	YACGTR	2 cut(s)	284, 606
BsaBI	GATNNNNATC	2 cut(s)	1245, 2416
BsaHI	GRCGYC	1 cut(s)	384
BsaI	GGTCTC	1 cut(s)	392
BsaJI	CCNNGG	5 cut(s)	1272, 1289, 2032, 2133, 2226
BsaWI	WCCGGW	4 cut(s)	440, 580, 618, 1572
Bsc4I	CCNNNNNNNGG	9 cut(s)	88, 276, 473, 839, 1554, 1613, 2500, 2501, 2525
Bse118I	RCCGGY	1 cut(s)	822
Bse1I	ACTGG	2 cut(s)	551, 2408
Bse21I	CCTNAGG	1 cut(s)	2055
Bse3DI	GCAATG	2 cut(s)	1914, 1956
Bse8I	GATNNNNATC	2 cut(s)	1245, 2416
BseAI	TCCGGA	2 cut(s)	580, 618
BseBI	CCWGG	2 cut(s)	1456, 1694
BseDI	CCNNGG	5 cut(s)	1272, 1289, 2032, 2133, 2226
BseGI	GGATG	5 cut(s)	33, 1495, 1689, 2002, 2272
BseJI	GATNNNNATC	2 cut(s)	1245, 2416
BseLI	CCNNNNNNNGG	9 cut(s)	88, 276, 473, 839, 1554, 1613, 2500, 2501, 2525
BseMI	GCAATG	2 cut(s)	1914, 1956
BseMII	CTCAG	7 cut(s)	387, 1388, 1599, 1617, 2046, 2208, 2346
BseNI	ACTGG	2 cut(s)	551, 2408
BseRI	GAGGAG	3 cut(s)	35, 139, 896
BseX3I	CGGCCG	1 cut(s)	824
BseXI	GCAGC	8 cut(s)	104, 657, 660, 816, 1312, 1609, 1979, 2245
BsgI	GTGCAG	3 cut(s)	23, 617, 2006
Bsh1236I	CGCG	5 cut(s)	653, 835, 837, 1375, 2278
Bsh1285I	CGRYCG	1 cut(s)	827
BshFI	GGCC	9 cut(s)	5, 826, 1321, 1617, 1631, 1765, 1882, 2315, 2493
BsiEI	CGRYCG	1 cut(s)	827
BsiHKAI	GWGCWC	4 cut(s)	324, 450, 588, 1203
BsiHKCI	CYCGRG	2 cut(s)	359, 1196
BslFI	GGGAC	5 cut(s)	242, 317, 926, 979, 2517
BslI	CCNNNNNNNGG	9 cut(s)	88, 276, 473, 839, 1554, 1613, 2500, 2501, 2525
BsmAI	GTCTC	1 cut(s)	392
BsmFI	GGGAC	5 cut(s)	242, 317, 926, 979, 2517
BsmI	GAATGC	2 cut(s)	1000, 1674
BsnI	GGCC	9 cut(s)	5, 826, 1321, 1617, 1631, 1765, 1882, 2315, 2493
Bso31I	GGTCTC	1 cut(s)	392
BsoBI	CYCGRG	2 cut(s)	359, 1196
Bsp119I	TTCGAA	2 cut(s)	66, 2346
Bsp1286I	GDGCHC	4 cut(s)	324, 450, 588, 1203
Bsp13I	TCCGGA	2 cut(s)	580, 618
Bsp1407I	TGTACA	2 cut(s)	555, 705
Bsp1720I	GCTNAGC	1 cut(s)	1049
Bsp19I	CCATGG	2 cut(s)	1289, 2226
BspANI	GGCC	9 cut(s)	5, 826, 1321, 1617, 1631, 1765, 1882, 2315, 2493
BspCNI	CTCAG	7 cut(s)	388, 1389, 1598, 1618, 2047, 2209, 2347
BspEI	TCCGGA	2 cut(s)	580, 618
BspFNI	CGCG	5 cut(s)	653, 835, 837, 1375, 2278
BspHI	TCATGA	1 cut(s)	288
BspMAI	CTGCAG	2 cut(s)	667, 2442
BspMI	ACCTGC	1 cut(s)	45
BspOI	GCTAGC	1 cut(s)	1041
BspQI	GCTCTTC	1 cut(s)	2343
BspT104I	TTCGAA	2 cut(s)	66, 2346
BspTNI	GGTCTC	1 cut(s)	392
BsrBI	CCGCTC	2 cut(s)	25, 616
BsrDI	GCAATG	2 cut(s)	1914, 1956
BsrFI	RCCGGY	1 cut(s)	822
BsrGI	TGTACA	2 cut(s)	555, 705
BsrI	ACTGG	2 cut(s)	551, 2408
BssAI	RCCGGY	1 cut(s)	822
BssECI	CCNNGG	5 cut(s)	1272, 1289, 2032, 2133, 2226
BssNI	GRCGYC	1 cut(s)	384
BssT1I	CCWWGG	5 cut(s)	1272, 1289, 2032, 2133, 2226
Bst2UI	CCWGG	2 cut(s)	1456, 1694
Bst6I	CTCTTC	2 cut(s)	1928, 2343
BstACI	GRCGYC	1 cut(s)	384
BstAPI	GCANNNNNTGC	2 cut(s)	1300, 1942
BstAUI	TGTACA	2 cut(s)	555, 705
BstBAI	YACGTR	2 cut(s)	284, 606
BstBI	TTCGAA	2 cut(s)	66, 2346
BstDSI	CCRYGG	2 cut(s)	1289, 2226
BstEII	GGTNACC	2 cut(s)	2036, 2444
BstF5I	GGATG	5 cut(s)	33, 1495, 1689, 2002, 2272
BstFNI	CGCG	5 cut(s)	653, 835, 837, 1375, 2278
BstH2I	RGCGCY	2 cut(s)	109, 396
BstHHI	GCGC	6 cut(s)	108, 343, 395, 655, 757, 837
BstMAI	GTCTC	1 cut(s)	392
BstMCI	CGRYCG	1 cut(s)	827
BstNI	CCWGG	2 cut(s)	1456, 1694
BstNSI	RCATGY	2 cut(s)	97, 1562
BstPAI	GACNNNNGTC	3 cut(s)	177, 269, 284
BstPI	GGTNACC	2 cut(s)	2036, 2444
BstSCI	CCNGG	9 cut(s)	275, 420, 455, 482, 983, 1454, 1692, 2040, 2500
BstSFI	CTRYAG	3 cut(s)	663, 1306, 2438
BstUI	CGCG	5 cut(s)	653, 835, 837, 1375, 2278
BstV1I	GCAGC	8 cut(s)	104, 657, 660, 816, 1312, 1609, 1979, 2245
BstV2I	GAAGAC	3 cut(s)	174, 1515, 2073
BstX2I	RGATCY	7 cut(s)	417, 577, 1543, 1703, 2171, 2335, 2543
BstXI	CCANNNNNNTGG	1 cut(s)	2140
BstYI	RGATCY	7 cut(s)	417, 577, 1543, 1703, 2171, 2335, 2543
BstZI	CGGCCG	1 cut(s)	824
Bsu36I	CCTNAGG	1 cut(s)	2055
BsuI	GTATCC	3 cut(s)	1452, 2053, 2469
BsuRI	GGCC	9 cut(s)	5, 826, 1321, 1617, 1631, 1765, 1882, 2315, 2493
BtgI	CCRYGG	2 cut(s)	1289, 2226
BtrI	CACGTC	1 cut(s)	370
BtsCI	GGATG	5 cut(s)	33, 1495, 1689, 2002, 2272
BtsI	GCAGTG	2 cut(s)	317, 655
BtsIMutI	CAGTG	8 cut(s)	317, 558, 655, 1224, 1303, 1592, 1973, 2214
BveI	ACCTGC	1 cut(s)	45
CaiI	CAGNNNCTG	1 cut(s)	1787
CciI	TCATGA	1 cut(s)	288
CfoI	GCGC	6 cut(s)	108, 343, 395, 655, 757, 837
Cfr10I	RCCGGY	1 cut(s)	822
Cfr13I	GGNCC	6 cut(s)	256, 476, 1319, 1575, 2313, 2492
CseI	GACGC	2 cut(s)	659, 2067
Csp6I	GTAC	8 cut(s)	556, 607, 643, 706, 871, 1494, 1959, 2205
CviQI	GTAC	8 cut(s)	556, 607, 643, 706, 871, 1494, 1959, 2205
DraIII	CACNNNGTG	1 cut(s)	799
DriI	GACNNNNNGTC	2 cut(s)	919, 2077
EaeI	YGGCCR	4 cut(s)	3, 824, 1615, 1763
EagI	CGGCCG	1 cut(s)	824
Eam1104I	CTCTTC	2 cut(s)	1928, 2343
Eam1105I	GACNNNNNGTC	2 cut(s)	919, 2077
EarI	CTCTTC	2 cut(s)	1928, 2343
EciI	GGCGGA	5 cut(s)	101, 151, 440, 728, 1696
Ecl136II	GAGCTC	1 cut(s)	448
EclXI	CGGCCG	1 cut(s)	824
Eco130I	CCWWGG	5 cut(s)	1272, 1289, 2032, 2133, 2226
Eco24I	GRGCYC	1 cut(s)	450
Eco31I	GGTCTC	1 cut(s)	392
Eco32I	GATATC	1 cut(s)	2297
Eco47I	GGWCC	3 cut(s)	256, 476, 1575
Eco47III	AGCGCT	1 cut(s)	107
Eco52I	CGGCCG	1 cut(s)	824
Eco53kI	GAGCTC	1 cut(s)	448
Eco57I	CTGAAG	1 cut(s)	1905
Eco72I	CACGTG	1 cut(s)	284
Eco81I	CCTNAGG	1 cut(s)	2055
Eco88I	CYCGRG	2 cut(s)	359, 1196
Eco91I	GGTNACC	2 cut(s)	2036, 2444
EcoICRI	GAGCTC	1 cut(s)	448
EcoO65I	GGTNACC	2 cut(s)	2036, 2444
EcoRII	CCWGG	2 cut(s)	1454, 1692
EcoRV	GATATC	1 cut(s)	2297
EcoT14I	CCWWGG	5 cut(s)	1272, 1289, 2032, 2133, 2226
EcoT22I	ATGCAT	2 cut(s)	1432, 1762
EcoT38I	GRGCYC	1 cut(s)	450
ErhI	CCWWGG	5 cut(s)	1272, 1289, 2032, 2133, 2226
FalI	AAGNNNNNCTT	4 cut(s)	308, 340, 926, 958
FaqI	GGGAC	5 cut(s)	242, 317, 926, 979, 2517
FauI	CCCGC	5 cut(s)	80, 315, 342, 565, 621
FauNDI	CATATG	2 cut(s)	1330, 1756
FokI	GGATG	5 cut(s)	40, 1502, 1676, 1989, 2259
FriOI	GRGCYC	1 cut(s)	450
FspBI	CTAG	1 cut(s)	1038
GlaI	GCGC	6 cut(s)	107, 342, 394, 654, 756, 836
GsuI	CTGGAG	1 cut(s)	2406
HaeII	RGCGCY	2 cut(s)	109, 396
HaeIII	GGCC	9 cut(s)	5, 826, 1321, 1617, 1631, 1765, 1882, 2315, 2493
HgaI	GACGC	2 cut(s)	659, 2067
HhaI	GCGC	6 cut(s)	108, 343, 395, 655, 757, 837
Hin1I	GRCGYC	1 cut(s)	384
Hin6I	GCGC	6 cut(s)	106, 341, 393, 653, 755, 835
HinP1I	GCGC	6 cut(s)	106, 341, 393, 653, 755, 835
HincII	GTYRAC	2 cut(s)	1026, 1531
HindII	GTYRAC	2 cut(s)	1026, 1531
HindIII	AAGCTT	1 cut(s)	1046
HpaI	GTTAAC	1 cut(s)	1531
HphI	GGTGA	7 cut(s)	115, 220, 238, 1078, 1250, 1901, 2438
Hpy166II	GTNNAC	8 cut(s)	531, 556, 706, 769, 1026, 1114, 1531, 2430
Hpy188I	TCNGA	9 cut(s)	19, 103, 407, 880, 1140, 1566, 1703, 2092, 2286
Hpy8I	GTNNAC	8 cut(s)	531, 556, 706, 769, 1026, 1114, 1531, 2430
Hpy99I	CGWCG	4 cut(s)	176, 188, 275, 386
HpyAV	CCTTC	7 cut(s)	310, 616, 694, 1375, 1399, 1880, 2479
HpyCH4IV	ACGT	8 cut(s)	207, 243, 283, 369, 384, 605, 1357, 2469
HpySE526I	ACGT	8 cut(s)	207, 243, 283, 369, 384, 605, 1357, 2469
Hsp92I	GRCGYC	1 cut(s)	384
HspAI	GCGC	6 cut(s)	106, 341, 393, 653, 755, 835
Kpn2I	TCCGGA	2 cut(s)	580, 618
KroI	GCCGGC	1 cut(s)	822
KroNI	GCCGGC	1 cut(s)	824
KspAI	GTTAAC	1 cut(s)	1531
LguI	GCTCTTC	1 cut(s)	2343
LmnI	GCTCC	8 cut(s)	22, 319, 583, 621, 635, 814, 1282, 1784
Lsp1109I	GCAGC	8 cut(s)	104, 657, 660, 816, 1312, 1609, 1979, 2245
LweI	GCATC	5 cut(s)	1417, 1458, 1645, 2065, 2329
MaeI	CTAG	1 cut(s)	1038
MaeII	ACGT	8 cut(s)	207, 243, 283, 369, 384, 605, 1357, 2469
MaeIII	GTNAC	6 cut(s)	179, 244, 250, 2036, 2304, 2444
MbiI	CCGCTC	2 cut(s)	25, 616
MflI	RGATCY	7 cut(s)	417, 577, 1543, 1703, 2171, 2335, 2543
MhlI	GDGCHC	4 cut(s)	324, 450, 588, 1203
MlsI	TGGCCA	1 cut(s)	1765
MluCI	AATT	9 cut(s)	351, 521, 1012, 1440, 1552, 1821, 1833, 1917, 2559
MluNI	TGGCCA	1 cut(s)	1765
MlyI	GAGTC	4 cut(s)	23, 497, 1577, 1991
MmeI	TCCRAC	6 cut(s)	669, 858, 1054, 1340, 2313, 2577
Mox20I	TGGCCA	1 cut(s)	1765
Mph1103I	ATGCAT	2 cut(s)	1432, 1762
MroI	TCCGGA	2 cut(s)	580, 618
MroNI	GCCGGC	1 cut(s)	822
MroXI	GAANNNNTTC	2 cut(s)	992, 2049
MscI	TGGCCA	1 cut(s)	1765
MseI	TTAA	5 cut(s)	354, 1233, 1530, 1817, 1836
MslI	CAYNNNNRTG	6 cut(s)	92, 186, 1419, 1425, 1759, 2277
Msp20I	TGGCCA	1 cut(s)	1765
MspA1I	CMGCKG	5 cut(s)	117, 337, 829, 1970, 2110
MspR9I	CCNGG	9 cut(s)	277, 422, 457, 484, 985, 1456, 1694, 2042, 2502
Mva1269I	GAATGC	2 cut(s)	1000, 1674
MvaI	CCWGG	2 cut(s)	1456, 1694
MvnI	CGCG	5 cut(s)	653, 835, 837, 1375, 2278
NaeI	GCCGGC	1 cut(s)	824
NciI	CCSGG	7 cut(s)	277, 422, 457, 484, 985, 2042, 2502
NcoI	CCATGG	2 cut(s)	1289, 2226
NdeI	CATATG	2 cut(s)	1330, 1756
NgoMIV	GCCGGC	1 cut(s)	822
NheI	GCTAGC	1 cut(s)	1037
NmeAIII	GCCGAG	5 cut(s)	439, 485, 834, 843, 2341
NmuCI	GTSAC	4 cut(s)	179, 244, 2304, 2444
NsiI	ATGCAT	2 cut(s)	1432, 1762
NspI	RCATGY	2 cut(s)	97, 1562
NspV	TTCGAA	2 cut(s)	66, 2346
OliI	CACNNNNGTG	1 cut(s)	186
PaeR7I	CTCGAG	1 cut(s)	359
PagI	TCATGA	1 cut(s)	288
PciI	ACATGT	1 cut(s)	93
PciSI	GCTCTTC	1 cut(s)	2343
PcsI	WCGNNNNNNNCGW	4 cut(s)	48, 180, 366, 411
PctI	GAATGC	2 cut(s)	1000, 1674
PdiI	GCCGGC	1 cut(s)	824
PdmI	GAANNNNTTC	2 cut(s)	992, 2049
PfeI	GAWTC	8 cut(s)	236, 432, 808, 1654, 1851, 1990, 2093, 2412
PflFI	GACNNNGTC	1 cut(s)	383
PfoI	TCCNGGA	2 cut(s)	482, 1454
PleI	GAGTC	4 cut(s)	22, 497, 1577, 1991
PmaCI	CACGTG	1 cut(s)	284
PmlI	CACGTG	1 cut(s)	284
PpsI	GAGTC	4 cut(s)	22, 497, 1577, 1991
Ppu21I	YACGTR	2 cut(s)	284, 606
PscI	ACATGT	1 cut(s)	93
PshAI	GACNNNNGTC	3 cut(s)	177, 269, 284
PsiI	TTATAA	3 cut(s)	1058, 1343, 1773
Psp124BI	GAGCTC	1 cut(s)	450
Psp1406I	AACGTT	1 cut(s)	1357
Psp6I	CCWGG	2 cut(s)	1454, 1692
PspCI	CACGTG	1 cut(s)	284
PspEI	GGTNACC	2 cut(s)	2036, 2444
PspGI	CCWGG	2 cut(s)	1454, 1692
PspPI	GGNCC	6 cut(s)	256, 476, 1319, 1575, 2313, 2492
PspXI	VCTCGAGB	1 cut(s)	359
PstI	CTGCAG	2 cut(s)	667, 2442
PstNI	CAGNNNCTG	1 cut(s)	1787
PsuI	RGATCY	7 cut(s)	417, 577, 1543, 1703, 2171, 2335, 2543
PsyI	GACNNNGTC	1 cut(s)	383
RsaI	GTAC	8 cut(s)	557, 608, 644, 707, 872, 1495, 1960, 2206
RsaNI	GTAC	8 cut(s)	556, 607, 643, 706, 871, 1494, 1959, 2205
RseI	CAYNNNNRTG	6 cut(s)	92, 186, 1419, 1425, 1759, 2277
SacI	GAGCTC	1 cut(s)	450
SapI	GCTCTTC	1 cut(s)	2343
SaqAI	TTAA	5 cut(s)	354, 1233, 1530, 1817, 1836
Sau96I	GGNCC	6 cut(s)	256, 476, 1319, 1575, 2313, 2492
SbfI	CCTGCAGG	1 cut(s)	667
ScaI	AGTACT	1 cut(s)	1960
SchI	GAGTC	4 cut(s)	23, 497, 1577, 1991
ScrFI	CCNGG	9 cut(s)	277, 422, 457, 484, 985, 1456, 1694, 2042, 2502
SdaI	CCTGCAGG	1 cut(s)	667
SduI	GDGCHC	4 cut(s)	324, 450, 588, 1203
SfaNI	GCATC	5 cut(s)	1417, 1458, 1645, 2065, 2329
SfcI	CTRYAG	3 cut(s)	663, 1306, 2438
Sfr274I	CTCGAG	1 cut(s)	359
SfuI	TTCGAA	2 cut(s)	66, 2346
SinI	GGWCC	3 cut(s)	256, 476, 1575
SlaI	CTCGAG	1 cut(s)	359
SmiMI	CAYNNNNRTG	6 cut(s)	92, 186, 1419, 1425, 1759, 2277
SmlI	CTYRAG	3 cut(s)	359, 866, 2007
SmoI	CTYRAG	3 cut(s)	359, 866, 2007
Sse8387I	CCTGCAGG	1 cut(s)	667
Sse9I	AATT	9 cut(s)	351, 521, 1012, 1440, 1552, 1821, 1833, 1917, 2559
SspI	AATATT	1 cut(s)	2166
SspMI	CTAG	1 cut(s)	1038
SstI	GAGCTC	1 cut(s)	450
StyD4I	CCNGG	9 cut(s)	275, 420, 455, 482, 983, 1454, 1692, 2040, 2500
StyI	CCWWGG	5 cut(s)	1272, 1289, 2032, 2133, 2226
TaiI	ACGT	8 cut(s)	210, 246, 286, 372, 387, 608, 1360, 2472
TaqI	TCGA	8 cut(s)	51, 66, 360, 519, 540, 1728, 2299, 2346
TasI	AATT	9 cut(s)	351, 521, 1012, 1440, 1552, 1821, 1833, 1917, 2559
TatI	WGTACW	4 cut(s)	555, 705, 870, 1958
TauI	GCSGC	8 cut(s)	78, 117, 805, 829, 835, 840, 843, 1973
TfiI	GAWTC	8 cut(s)	236, 432, 808, 1654, 1851, 1990, 2093, 2412
Tru1I	TTAA	5 cut(s)	354, 1233, 1530, 1817, 1836
Tru9I	TTAA	5 cut(s)	354, 1233, 1530, 1817, 1836
TscAI	CASTG	8 cut(s)	317, 558, 662, 1231, 1310, 1599, 1980, 2221
TseFI	GTSAC	4 cut(s)	179, 244, 2304, 2444
TseI	GCWGC	8 cut(s)	117, 670, 673, 829, 1325, 1622, 1967, 2258
Tsp45I	GTSAC	4 cut(s)	179, 244, 2304, 2444
TspGWI	ACGGA	4 cut(s)	26, 489, 622, 764
TspRI	CASTG	8 cut(s)	317, 558, 662, 1231, 1310, 1599, 1980, 2221
Tth111I	GACNNNGTC	1 cut(s)	383
VpaK11BI	GGWCC	3 cut(s)	256, 476, 1575
XapI	RAATTY	2 cut(s)	1917, 2559
XceI	RCATGY	2 cut(s)	97, 1562
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1287
XhoI	CTCGAG	1 cut(s)	359
XmnI	GAANNNNTTC	2 cut(s)	992, 2049
XspI	CTAG	1 cut(s)	1038
ZraI	GACGTC	1 cut(s)	385
ZrmI	AGTACT	1 cut(s)	1960
Zsp2I	ATGCAT	2 cut(s)	1432, 1762

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.