Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0000855

Physical Location & Seq

Genomic Coordinates Forward (+)

82080238 .. 82086158

Gene Structure 5921 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00144090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2619 bp

ATGGCGGAACCGACGTCGTCGGATCAGATAGTCTATCTGCACGGCGACCTGGACCTCCAAATCATCGAGGCGCGCTACCTGCCCAACATGGACATCGTCTCCGAGCGCCTCCGCCGCTGCTTCACCGCCTGCGACACCGTCAACTGCACCGGCTCCCGCTCCGACAATCCCGATGCCGGCCACGGAGCCGAGGACCGCAAGAAGGTCCACCACCGCAAAATCATCACCAGCGACTCTTACGTCAGCGTCGTTGTGCCCCAGGCCACGGTGGCGCGTACGCGCGTCATCAAGAACTCGCAGAATCCTCAGTGGAAAGAGACCTTTTCCATCCCGCTCGCGCATCCCGCGGCTAAGGTTGAGTTTCAGGTCAAAGATAACGACCTCTTCGGCGCTGAGCTTATCGGCACCGCCTCGATCCCCGCCGCCGAAATCGCCTCCGGAAAATCCATTTCCGGCTGGTTTGAGATTATCGGACCCACCGGAAAGCCTCCGAAGCCTAATTGCGCTATTAAGGTCGAGTTGAAGTTCACTCCCTTCGAGAAAAACCCGGTCTACAGGCACGGCATCGCCGGCGATCCAGAACGGAAGGGAGTCCCCAAGACCTACTTCCCTATGAGGAAGGGAAGTCACGTGAGGCTGTACCAGGACGCGCACGTGCCGGAGGGGATGCTGCCGCAGATTGAATTGGACGGAAGGAAGGTTTATAAGCCTGAGAATTGCTGGGAGGACATTTGCTATGCGATTTCGGAGGCTCACCATATGATCTATGTGGTGGGATGGTCTGTGTTTTACAAAGTGAGGCTGGTTAGGGAGCCGAGCCGGAAGTTGCCACGCGGCGGCGATTTGACGCTCGGAGAGTTGCTCAAGTATAAGTCGGAAGAAGGCGTACGGGTGTTGTTGCTGGTCTGGGATGATAAGACCTCCCACGACAAGTTCTTCTTCAAATCGGCAGGAATGATGCAAACTCATGATGAAGAAACCCGGAAGTTTTTCAAGCATTCATCTGTCACTTGTGTTCTGGCCCCTCGCTATGGCAGTAGTAAGCTTAGCATTATGAAACAACAGGCAGGTTCCAGTTCTTACTTTTTTGTTGTTGGAACCCTCTTTACACACCATCAAAAATGTGTTATTGTGGACACACAAGCATCTGGTAATAACCGCAAGATAACTACATTTTTAGGAGGTCTTGATCTCTGTGATGGTCGTTATGACACACCTGAGCACAGATTGTTTCGGGATCTCGACACTGTGTTTAAAGAAGATTTTCATCAACCTACGTTTGCTGCTGGGACCAAGGCTCCAAGGCAACCATGGCATGACTTGCACTGTAGAATTGACGGGCCTGCTGCATATGATGTTCTTATAAACTTTGAGCAACGGTGGAGGAGAGCAACACAGTGGAGGGAGTTTGGACTACGTAAAAAGGTTTCGCATTGGCATGATGATGCTTTAATTAAGATAGACCGTATTTCATGGATACTTAGTCCTAAGATGTCGCATTCAAAGGAGTCAAAGGAGTCAAAGGATGTTACAATTGTTCCCGAAGATGACCCAGCTTTATGGGTTCAGAGAGAAGATGATCCCGAAGATTGGCATGTTCAGATTTTCAGGTCTATTGACTCGGGATCACTGAAAGGATTTCCCAAAGATGTCATTCATGCTCAGTCAAAGAACCTTATTTGCTCAAAGGATCTAGTAATAGATAAAAGCATCCAGAAAGCATACATCAATGCAATCAGATCTGCCCAACATTTCATATATATTGAAAATCAGTATTTTCTTGGTTCATCATATGCATGGCCAGATTATAAAAATGCAGGAGCTGACAATCTTATCCCAATGGAGTTGGCACTAAAGATTGCCAGCAAAATTAGAGCTAATGAGAGATTTGCAGTGTATATAGTTCTACCTATGTGGCCTGAAGGTGATCCGAAAACTGGTGCAATGCAAGAAATTCTCTACTGGCAGAGCCAAACAATGCAAATGATGTATGGTATTGTTGCACGGGAATTGAGGGCCCCGCAGCTTCAGGACTTACATCCTCAAGATTTCCTCAATTTCTATTGCCTTGGTAATCGGGAAAAACTTTCTGAGGAAACATCAAACAACAATGGTGCTACGATCTCCGATGCATACAAGTACCAGCGCTTTATGATTTATGTTCATGCCAAGGGTATGATAGTAGATGACGAGTACGTTATAGTGGGATCTGCCAATATCAACCAAAGATCAATGGCCGGTACAAAAGACACTGAGATAGCTATGGGTGCGTATCAACCCCATCACAGTTGGGCTGAGAAGAAGAGACATCCATGTGGTCAGATATATGGGTATCGAATGTCGCTGTGGGCTGAGCATCTTGGTAAGTCGGACCCATGCTTCAAAGAGGCAGAGAGTTTGGAATGTGTGAGGAATGTGAATGGCATTGCCGAAGAAAACTGGAGGAGATATACATTACTGGATTTCACAGAACTGCAGGGTCACCTTCTCAGGTACCCATTGGAGGTAGATAGACATGGTAACGTTAAACCCCTTCCAGGACATGAAAATTTCCCTGATGTTGGTGGCAAAGTTATTGGAGCTCATTCTGCTACACTTCCTGATCAGTTGACAACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006950 GO:0008081 GO:0008150 GO:0008152 GO:0008219 GO:0009266 GO:0009409 GO:0009506 GO:0009628 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010119 GO:0010646 GO:0010647 GO:0012501 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019637 GO:0023051 GO:0023056 GO:0030054 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046473 GO:0046486 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0090333 GO:1901419 GO:1901421 GO:1905957 GO:1905959

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

872

Amino Acids

99.39

Weight (kDa)

7.41

Isoelectric Point (pI)

32.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	75 - 155	7.5e-16	C2 domain
PLDc_2	PF13091	712 - 755	1.2e-06	PLD-like domain
PLDc	PF00614	719 - 744	3.2e-08	Phospholipase D Active site motif
PLD_C	PF12357	792 - 861	1e-27	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000855)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35790 AT4G35790 AT4G35790
fragaria_vesca	FvH4_1g11000 FvH4_1g11020 FvH4_1g11020 FvH4_2g37900 FvH4_5g01272 FvH4_5g28780 FvH4_5g28800 FvH4_7g08510 FvH4_7g08510
malus_domestica	MD02G1123100.v1.1 MD08G1149700.v1.1 MD08G1237200.v1.1 MD15G1006400.v1.1 MD15G1237100.v1.1
prunus_persica	Prupe.1G359000_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.7G175400_v2.0.a1
pyrus_communis	pycom02g09600 pycom15g00480 pycom15g21090
rosa_chinensis	RchiOBHm_Chr2g0098271 RchiOBHm_Chr6g0303711 RchiOBHm_Chr7g0221001 RchiOBHm_Chr7g0221011
rosa_laevigata	RLG00000002185 RLG00000011053 RLG00000016781
rosa_multiflora	Rmu_co8414259.1_g000001 Rmu_co8436567.1_g000002 Rmu_co8452585.1_g000001 Rmu_sc0001024.1_g000003 Rmu_sc0002105.1_g000010 Rmu_sc0002105.1_g000018 Rmu_sc0002105.1_g000020 Rmu_sc0009922.1_g000005 Rmu_sc0039265.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144090 Rroxscaffold_3G00238820 Rroxscaffold_7G00164460
rosa_rugosa	Rorug02G0071800 Rorug06G0324200 Rorug07G0199200 Rorug07G0199300 Rorug07G0199400 Rorug07G0199500 Rorug07G0199600
rosa_samantha	Rh1CG003000 Rh2AG118900 Rh2BG121800 Rh2CG123400 Rh2DG123500 Rh6AG438500 Rh6BG464500 Rh6CG450800 Rh6DG437500 Rh7AG339200 Rh7BG329900 Rh7CG357200 Rh7DG337900
rosa_wichuraiana	Rw2G009320 Rw6G037950 Rw7G028850

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	705, 1364, 1809
AasI	GACNNNNNNGTC	1 cut(s)	239
AatII	GACGTC	1 cut(s)	17
Acc36I	ACCTGC	2 cut(s)	87, 1058
Acc65I	GGTACC	1 cut(s)	2493
AccB1I	GGYRCC	2 cut(s)	404, 2493
AccBSI	CCGCTC	2 cut(s)	159, 334
AccI	GTMKAC	1 cut(s)	552
AccII	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
AccIII	TCCGGA	1 cut(s)	437
AclI	AACGTT	1 cut(s)	2523
AclWI	GGATC	9 cut(s)	30, 409, 569, 1245, 1574, 1633, 1698, 1922, 2215
AcoI	YGGCCR	3 cut(s)	178, 1799, 2235
AcsI	RAATTY	2 cut(s)	1953, 2548
AcuI	CTGAAG	2 cut(s)	1941, 2012
AcvI	CACGTG	2 cut(s)	631, 655
AcyI	GRCGYC	1 cut(s)	14
AfaI	GTAC	7 cut(s)	277, 641, 888, 2141, 2195, 2242, 2495
AfeI	AGCGCT	1 cut(s)	2147
AgsI	TTSAA	7 cut(s)	523, 683, 943, 994, 1503, 1766, 2383
AjnI	CCWGG	4 cut(s)	48, 258, 642, 2536
AjuI	GAANNNNNNNTTGG	2 cut(s)	590, 622
AluBI	AGCT	8 cut(s)	397, 1045, 1556, 1823, 1877, 2026, 2261, 2582
AluI	AGCT	8 cut(s)	397, 1045, 1556, 1823, 1877, 2026, 2261, 2582
Alw21I	GWGCWC	2 cut(s)	1224, 2584
Alw26I	GTCTC	3 cut(s)	103, 311, 2299
AlwI	GGATC	9 cut(s)	30, 409, 569, 1245, 1574, 1633, 1698, 1922, 2215
AlwNI	CAGNNNCTG	1 cut(s)	1823
Ama87I	CYCGRG	1 cut(s)	1621
Aor13HI	TCCGGA	1 cut(s)	437
Aor51HI	AGCGCT	1 cut(s)	2147
AoxI	GGCC	8 cut(s)	178, 261, 1020, 1340, 1799, 1916, 2016, 2235
ApaI	GGGCCC	1 cut(s)	2020
ApeKI	GCWGC	5 cut(s)	117, 670, 1283, 1346, 2023
ApoI	RAATTY	2 cut(s)	1953, 2548
Asp700I	GAANNNNTTC	2 cut(s)	989, 1263
Asp718I	GGTACC	1 cut(s)	2493
AsuC2I	CCSGG	2 cut(s)	548, 982
AsuHPI	GGTGA	5 cut(s)	115, 217, 746, 1937, 2474
AvaI	CYCGRG	1 cut(s)	1621
AvaII	GGWCC	6 cut(s)	52, 193, 205, 473, 1290, 2371
BaeGI	GKGCMC	2 cut(s)	258, 2020
BalI	TGGCCA	1 cut(s)	1801
BanI	GGYRCC	2 cut(s)	404, 2493
BanII	GRGCYC	2 cut(s)	2020, 2584
BarI	GAAGNNNNNNTAC	2 cut(s)	870, 902
BbrPI	CACGTG	2 cut(s)	631, 655
Bbv12I	GWGCWC	2 cut(s)	1224, 2584
BbvI	GCAGC	5 cut(s)	104, 657, 1270, 1333, 2035
BccI	CCATC	5 cut(s)	335, 771, 1122, 1193, 2289
BceAI	ACGGC	2 cut(s)	58, 577
BciT130I	CCWGG	4 cut(s)	50, 260, 644, 2538
BciVI	GTATCC	1 cut(s)	1470
BclI	TGATCA	1 cut(s)	2602
BcnI	CCSGG	2 cut(s)	548, 982
BcoDI	GTCTC	3 cut(s)	103, 311, 2299
BfaI	CTAG	1 cut(s)	1694
BfmI	CTRYAG	3 cut(s)	553, 1327, 2474
BfoI	RGCGCY	3 cut(s)	109, 393, 2149
BfuAI	ACCTGC	2 cut(s)	87, 1058
BfuI	GTATCC	1 cut(s)	1470
BglI	GCCNNNNNGGC	1 cut(s)	269
BglII	AGATCT	1 cut(s)	1739
BlpI	GCTNAGC	3 cut(s)	393, 1046, 2352
Bme1390I	CCNGG	6 cut(s)	50, 260, 548, 644, 982, 2538
Bme18I	GGWCC	6 cut(s)	52, 193, 205, 473, 1290, 2371
BmeT110I	CYCGRG	1 cut(s)	1621
BmrFI	CCNGG	6 cut(s)	50, 260, 548, 644, 982, 2538
BplI	GAGNNNNNCTC	2 cut(s)	846, 878
BpmI	CTGGAG	1 cut(s)	2461
Bpu10I	CCTNAGC	2 cut(s)	351, 1218
Bpu1102I	GCTNAGC	3 cut(s)	393, 1046, 2352
BpuEI	CTTGAG	2 cut(s)	848, 2028
BpuMI	CCSGG	2 cut(s)	548, 982
BsaAI	YACGTR	3 cut(s)	631, 655, 1418
BsaBI	GATNNNNATC	1 cut(s)	1266
BsaHI	GRCGYC	1 cut(s)	14
BsaI	GGTCTC	1 cut(s)	311
BsaWI	WCCGGW	2 cut(s)	437, 479
BsaXI	ACNNNNNCTCC	4 cut(s)	83, 113, 1282, 1312
Bse118I	RCCGGY	4 cut(s)	149, 176, 569, 2237
Bse1I	ACTGG	5 cut(s)	1074, 1942, 1967, 2444, 2463
Bse3DI	GCAATG	2 cut(s)	1950, 2424
Bse8I	GATNNNNATC	1 cut(s)	1266
BseAI	TCCGGA	1 cut(s)	437
BseBI	CCWGG	4 cut(s)	50, 260, 644, 2538
BseGI	GGATG	9 cut(s)	327, 340, 672, 782, 916, 1531, 1710, 2038, 2308
BseJI	GATNNNNATC	1 cut(s)	1266
BseMI	GCAATG	2 cut(s)	1950, 2424
BseNI	ACTGG	5 cut(s)	1074, 1942, 1967, 2444, 2463
BsePI	GCGCGC	1 cut(s)	71
BseRI	GAGGAG	2 cut(s)	1399, 2458
BseSI	GKGCMC	2 cut(s)	258, 2020
BseXI	GCAGC	5 cut(s)	104, 657, 1270, 1333, 2035
BseYI	CCCAGC	3 cut(s)	720, 1286, 1552
BsgI	GTGCAG	2 cut(s)	23, 130
Bsh1236I	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BshFI	GGCC	8 cut(s)	180, 263, 1022, 1342, 1801, 1918, 2018, 2237
BshNI	GGYRCC	2 cut(s)	404, 2493
BsiHKAI	GWGCWC	2 cut(s)	1224, 2584
BsiHKCI	CYCGRG	1 cut(s)	1621
BsiWI	CGTACG	2 cut(s)	275, 886
BslFI	GGGAC	2 cut(s)	578, 1303
BsmAI	GTCTC	3 cut(s)	103, 311, 2299
BsmBI	CGTCTC	1 cut(s)	103
BsmFI	GGGAC	2 cut(s)	578, 1303
BsmI	GAATGC	2 cut(s)	997, 1498
BsnI	GGCC	8 cut(s)	180, 263, 1022, 1342, 1801, 1918, 2018, 2237
Bso31I	GGTCTC	1 cut(s)	311
BsoBI	CYCGRG	1 cut(s)	1621
Bsp120I	GGGCCC	1 cut(s)	2016
Bsp1286I	GDGCHC	4 cut(s)	258, 1224, 2020, 2584
Bsp13I	TCCGGA	1 cut(s)	437
Bsp1720I	GCTNAGC	3 cut(s)	393, 1046, 2352
Bsp19I	CCATGG	1 cut(s)	1310
BspANI	GGCC	8 cut(s)	180, 263, 1022, 1342, 1801, 1918, 2018, 2237
BspEI	TCCGGA	1 cut(s)	437
BspFNI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BspHI	TCATGA	1 cut(s)	967
BspMAI	CTGCAG	1 cut(s)	2478
BspMI	ACCTGC	2 cut(s)	87, 1058
BspPI	GGATC	9 cut(s)	30, 409, 569, 1245, 1574, 1633, 1698, 1922, 2215
BspT107I	GGYRCC	2 cut(s)	404, 2493
BspTNI	GGTCTC	1 cut(s)	311
BsrBI	CCGCTC	2 cut(s)	159, 334
BsrDI	GCAATG	2 cut(s)	1950, 2424
BsrFI	RCCGGY	4 cut(s)	149, 176, 569, 2237
BsrI	ACTGG	5 cut(s)	1074, 1942, 1967, 2444, 2463
BssAI	RCCGGY	4 cut(s)	149, 176, 569, 2237
BssHII	GCGCGC	1 cut(s)	71
BssNI	GRCGYC	1 cut(s)	14
BssT1I	CCWWGG	5 cut(s)	1293, 1301, 1310, 2068, 2169
Bst2UI	CCWGG	4 cut(s)	50, 260, 644, 2538
Bst4CI	ACNGT	8 cut(s)	139, 268, 1249, 1328, 1380, 1398, 1466, 2288
Bst6I	CTCTTC	2 cut(s)	389, 2297
BstACI	GRCGYC	1 cut(s)	14
BstAPI	GCANNNNNTGC	1 cut(s)	1321
BstBAI	YACGTR	3 cut(s)	631, 655, 1418
BstC8I	GCNNGC	7 cut(s)	73, 130, 178, 336, 571, 1344, 1864
BstDSI	CCRYGG	4 cut(s)	181, 264, 345, 1310
BstEII	GGTNACC	1 cut(s)	2480
BstF5I	GGATG	9 cut(s)	327, 340, 672, 782, 916, 1531, 1710, 2038, 2308
BstFNI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BstH2I	RGCGCY	3 cut(s)	109, 393, 2149
BstMAI	GTCTC	3 cut(s)	103, 311, 2299
BstNI	CCWGG	4 cut(s)	50, 260, 644, 2538
BstNSI	RCATGY	1 cut(s)	1598
BstPI	GGTNACC	1 cut(s)	2480
BstSCI	CCNGG	6 cut(s)	48, 258, 546, 642, 980, 2536
BstSFI	CTRYAG	3 cut(s)	553, 1327, 2474
BstSLI	GKGCMC	2 cut(s)	258, 2020
BstSNI	TACGTA	1 cut(s)	1418
BstUI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BstV1I	GCAGC	5 cut(s)	104, 657, 1270, 1333, 2035
BstX2I	RGATCY	4 cut(s)	1237, 1690, 1739, 2207
BstXI	CCANNNNNNTGG	1 cut(s)	1560
BstYI	RGATCY	4 cut(s)	1237, 1690, 1739, 2207
BsuI	GTATCC	1 cut(s)	1470
BsuRI	GGCC	8 cut(s)	180, 263, 1022, 1342, 1801, 1918, 2018, 2237
BtgI	CCRYGG	4 cut(s)	181, 264, 345, 1310
BtgZI	GCGATG	1 cut(s)	550
BtsCI	GGATG	9 cut(s)	327, 340, 672, 782, 916, 1531, 1710, 2038, 2308
BtsI	GCAGTG	1 cut(s)	1899
BtsIMutI	CAGTG	7 cut(s)	314, 1245, 1324, 1403, 1628, 1899, 2250
BveI	ACCTGC	2 cut(s)	87, 1058
Cac8I	GCNNGC	7 cut(s)	73, 130, 178, 336, 571, 1344, 1864
CaiI	CAGNNNCTG	1 cut(s)	1823
CciI	TCATGA	1 cut(s)	967
Cfr10I	RCCGGY	4 cut(s)	149, 176, 569, 2237
Cfr42I	CCGCGG	1 cut(s)	348
CseI	GACGC	4 cut(s)	235, 271, 656, 856
Csp6I	GTAC	7 cut(s)	276, 640, 887, 2140, 2194, 2241, 2494
CviQI	GTAC	7 cut(s)	276, 640, 887, 2140, 2194, 2241, 2494
DraI	TTTAAA	1 cut(s)	1255
DrdI	GACNNNNNNGTC	1 cut(s)	239
DseDI	GACNNNNNNGTC	1 cut(s)	239
EaeI	YGGCCR	3 cut(s)	178, 1799, 2235
Eam1104I	CTCTTC	2 cut(s)	389, 2297
EarI	CTCTTC	2 cut(s)	389, 2297
EciI	GGCGGA	2 cut(s)	20, 101
Ecl136II	GAGCTC	1 cut(s)	2582
Eco105I	TACGTA	1 cut(s)	1418
Eco130I	CCWWGG	5 cut(s)	1293, 1301, 1310, 2068, 2169
Eco24I	GRGCYC	2 cut(s)	2020, 2584
Eco31I	GGTCTC	1 cut(s)	311
Eco47I	GGWCC	6 cut(s)	52, 193, 205, 473, 1290, 2371
Eco47III	AGCGCT	1 cut(s)	2147
Eco53kI	GAGCTC	1 cut(s)	2582
Eco57I	CTGAAG	2 cut(s)	1941, 2012
Eco72I	CACGTG	2 cut(s)	631, 655
Eco88I	CYCGRG	1 cut(s)	1621
Eco91I	GGTNACC	1 cut(s)	2480
EcoICRI	GAGCTC	1 cut(s)	2582
EcoO109I	RGGNCCY	2 cut(s)	2016, 2017
EcoO65I	GGTNACC	1 cut(s)	2480
EcoRII	CCWGG	4 cut(s)	48, 258, 642, 2536
EcoT14I	CCWWGG	5 cut(s)	1293, 1301, 1310, 2068, 2169
EcoT22I	ATGCAT	2 cut(s)	1798, 2134
EcoT38I	GRGCYC	2 cut(s)	2020, 2584
ErhI	CCWWGG	5 cut(s)	1293, 1301, 1310, 2068, 2169
Esp3I	CGTCTC	1 cut(s)	103
FalI	AAGNNNNNCTT	4 cut(s)	590, 622, 923, 955
FaqI	GGGAC	2 cut(s)	578, 1303
FauI	CCCGC	5 cut(s)	164, 339, 352, 427, 2028
FauNDI	CATATG	3 cut(s)	759, 1351, 1792
FbaI	TGATCA	1 cut(s)	2602
FblI	GTMKAC	1 cut(s)	552
FokI	GGATG	9 cut(s)	314, 327, 679, 789, 923, 1538, 1697, 2025, 2295
FriOI	GRGCYC	2 cut(s)	2020, 2584
FspBI	CTAG	1 cut(s)	1694
GsaI	CCCAGC	3 cut(s)	724, 1290, 1556
GsuI	CTGGAG	1 cut(s)	2461
HaeII	RGCGCY	3 cut(s)	109, 393, 2149
HaeIII	GGCC	8 cut(s)	180, 263, 1022, 1342, 1801, 1918, 2018, 2237
HgaI	GACGC	4 cut(s)	235, 271, 656, 856
Hin1I	GRCGYC	1 cut(s)	14
HincII	GTYRAC	2 cut(s)	142, 2610
HindII	GTYRAC	2 cut(s)	142, 2610
HindIII	AAGCTT	1 cut(s)	1043
HinfI	GANTC	6 cut(s)	233, 301, 591, 1508, 1517, 1619
HphI	GGTGA	5 cut(s)	115, 217, 746, 1937, 2474
Hpy166II	GTNNAC	6 cut(s)	142, 208, 528, 553, 1135, 2610
Hpy8I	GTNNAC	6 cut(s)	142, 208, 528, 553, 1135, 2610
Hpy99I	CGWCG	4 cut(s)	16, 19, 22, 251
HpyCH4III	ACNGT	8 cut(s)	139, 268, 1249, 1328, 1380, 1398, 1466, 2288
HpyCH4IV	ACGT	8 cut(s)	14, 240, 630, 654, 1277, 1417, 2196, 2523
HpySE526I	ACGT	8 cut(s)	14, 240, 630, 654, 1277, 1417, 2196, 2523
Hsp92I	GRCGYC	1 cut(s)	14
Kpn2I	TCCGGA	1 cut(s)	437
KpnI	GGTACC	1 cut(s)	2497
KroI	GCCGGC	2 cut(s)	176, 569
KroNI	GCCGGC	2 cut(s)	178, 571
Ksp22I	TGATCA	1 cut(s)	2602
KspI	CCGCGG	1 cut(s)	348
LmnI	GCTCC	7 cut(s)	158, 164, 185, 811, 1303, 1820, 2579
Lsp1109I	GCAGC	5 cut(s)	104, 657, 1270, 1333, 2035
MaeI	CTAG	1 cut(s)	1694
MaeII	ACGT	8 cut(s)	14, 240, 630, 654, 1277, 1417, 2196, 2523
MaeIII	GTNAC	5 cut(s)	626, 1006, 1528, 2480, 2519
MbiI	CCGCTC	2 cut(s)	159, 334
MfeI	CAATTG	1 cut(s)	1533
MflI	RGATCY	4 cut(s)	1237, 1690, 1739, 2207
MhlI	GDGCHC	4 cut(s)	258, 1224, 2020, 2584
MlsI	TGGCCA	1 cut(s)	1801
MluNI	TGGCCA	1 cut(s)	1801
MlyI	GAGTC	5 cut(s)	227, 600, 1517, 1526, 1613
MmeI	TCCRAC	4 cut(s)	186, 855, 1075, 2349
Mox20I	TGGCCA	1 cut(s)	1801
Mph1103I	ATGCAT	2 cut(s)	1798, 2134
MreI	CGCCGGCG	1 cut(s)	569
MroI	TCCGGA	1 cut(s)	437
MroNI	GCCGGC	2 cut(s)	176, 569
MroXI	GAANNNNTTC	2 cut(s)	989, 1263
MscI	TGGCCA	1 cut(s)	1801
MseI	TTAA	5 cut(s)	510, 1254, 1451, 1455, 2526
MslI	CAYNNNNRTG	4 cut(s)	1437, 1443, 1795, 2313
Msp20I	TGGCCA	1 cut(s)	1801
MspA1I	CMGCKG	2 cut(s)	117, 347
MspR9I	CCNGG	6 cut(s)	50, 260, 548, 644, 982, 2538
MunI	CAATTG	1 cut(s)	1533
Mva1269I	GAATGC	2 cut(s)	997, 1498
MvaI	CCWGG	4 cut(s)	50, 260, 644, 2538
MvnI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
NaeI	GCCGGC	2 cut(s)	178, 571
NciI	CCSGG	2 cut(s)	548, 982
NcoI	CCATGG	1 cut(s)	1310
NdeI	CATATG	3 cut(s)	759, 1351, 1792
NgoMIV	GCCGGC	2 cut(s)	176, 569
NmeAIII	GCCGAG	2 cut(s)	214, 840
NmuCI	GTSAC	3 cut(s)	626, 1006, 2480
NsiI	ATGCAT	2 cut(s)	1798, 2134
NspI	RCATGY	1 cut(s)	1598
PacI	TTAATTAA	1 cut(s)	1455
PagI	TCATGA	1 cut(s)	967
PauI	GCGCGC	1 cut(s)	71
PcsI	WCGNNNNNNNCGW	1 cut(s)	246
PctI	GAATGC	2 cut(s)	997, 1498
PdiI	GCCGGC	2 cut(s)	178, 571
PdmI	GAANNNNTTC	2 cut(s)	989, 1263
PfeI	GAWTC	1 cut(s)	301
Pfl23II	CGTACG	2 cut(s)	275, 886
PflFI	GACNNNGTC	3 cut(s)	16, 95, 137
PfoI	TCCNGGA	1 cut(s)	2536
PleI	GAGTC	5 cut(s)	227, 599, 1516, 1525, 1613
PmaCI	CACGTG	2 cut(s)	631, 655
PmlI	CACGTG	2 cut(s)	631, 655
PpsI	GAGTC	5 cut(s)	227, 599, 1516, 1525, 1613
Ppu21I	YACGTR	3 cut(s)	631, 655, 1418
PsiI	TTATAA	3 cut(s)	705, 1364, 1809
Psp124BI	GAGCTC	1 cut(s)	2584
Psp1406I	AACGTT	1 cut(s)	2523
Psp6I	CCWGG	4 cut(s)	48, 258, 642, 2536
PspCI	CACGTG	2 cut(s)	631, 655
PspEI	GGTNACC	1 cut(s)	2480
PspFI	CCCAGC	3 cut(s)	720, 1286, 1552
PspGI	CCWGG	4 cut(s)	48, 258, 642, 2536
PspLI	CGTACG	2 cut(s)	275, 886
PspOMI	GGGCCC	1 cut(s)	2016
PsrI	GAACNNNNNNTAC	2 cut(s)	1090, 1122
PstI	CTGCAG	1 cut(s)	2478
PstNI	CAGNNNCTG	1 cut(s)	1823
PsuI	RGATCY	4 cut(s)	1237, 1690, 1739, 2207
PsyI	GACNNNGTC	3 cut(s)	16, 95, 137
PteI	GCGCGC	1 cut(s)	71
RsaI	GTAC	7 cut(s)	277, 641, 888, 2141, 2195, 2242, 2495
RsaNI	GTAC	7 cut(s)	276, 640, 887, 2140, 2194, 2241, 2494
RseI	CAYNNNNRTG	4 cut(s)	1437, 1443, 1795, 2313
SacI	GAGCTC	1 cut(s)	2584
SacII	CCGCGG	1 cut(s)	348
SaqAI	TTAA	5 cut(s)	510, 1254, 1451, 1455, 2526
SchI	GAGTC	5 cut(s)	227, 600, 1517, 1526, 1613
ScrFI	CCNGG	6 cut(s)	50, 260, 548, 644, 982, 2538
SduI	GDGCHC	4 cut(s)	258, 1224, 2020, 2584
SfcI	CTRYAG	3 cut(s)	553, 1327, 2474
Sfr303I	CCGCGG	1 cut(s)	348
SgrAI	CRCCGGYG	1 cut(s)	569
SgrBI	CCGCGG	1 cut(s)	348
SinI	GGWCC	6 cut(s)	52, 193, 205, 473, 1290, 2371
SmiMI	CAYNNNNRTG	4 cut(s)	1437, 1443, 1795, 2313
SmlI	CTYRAG	2 cut(s)	863, 2043
SmoI	CTYRAG	2 cut(s)	863, 2043
SnaBI	TACGTA	1 cut(s)	1418
SspMI	CTAG	1 cut(s)	1694
SstI	GAGCTC	1 cut(s)	2584
StyD4I	CCNGG	6 cut(s)	48, 258, 546, 642, 980, 2536
StyI	CCWWGG	5 cut(s)	1293, 1301, 1310, 2068, 2169
TaaI	ACNGT	8 cut(s)	139, 268, 1249, 1328, 1380, 1398, 1466, 2288
TaiI	ACGT	8 cut(s)	17, 243, 633, 657, 1280, 1420, 2199, 2526
TaqI	TCGA	6 cut(s)	66, 413, 516, 537, 1242, 2335
TauI	GCSGC	6 cut(s)	117, 350, 425, 676, 837, 840
TfiI	GAWTC	1 cut(s)	301
Tru1I	TTAA	5 cut(s)	510, 1254, 1451, 1455, 2526
Tru9I	TTAA	5 cut(s)	510, 1254, 1451, 1455, 2526
TscAI	CASTG	7 cut(s)	314, 1252, 1331, 1403, 1635, 1899, 2257
TseFI	GTSAC	3 cut(s)	626, 1006, 2480
TseI	GCWGC	5 cut(s)	117, 670, 1283, 1346, 2023
Tsp45I	GTSAC	3 cut(s)	626, 1006, 2480
TspGWI	ACGGA	3 cut(s)	198, 598, 705
TspRI	CASTG	7 cut(s)	314, 1252, 1331, 1403, 1635, 1899, 2257
Tth111I	GACNNNGTC	3 cut(s)	16, 95, 137
VpaK11BI	GGWCC	6 cut(s)	52, 193, 205, 473, 1290, 2371
XapI	RAATTY	2 cut(s)	1953, 2548
XceI	RCATGY	1 cut(s)	1598
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1308
XmiI	GTMKAC	1 cut(s)	552
XmnI	GAANNNNTTC	2 cut(s)	989, 1263
XspI	CTAG	1 cut(s)	1694
ZraI	GACGTC	1 cut(s)	15
Zsp2I	ATGCAT	2 cut(s)	1798, 2134

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.