Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000855

Physical Location & Seq

Genomic Coordinates Reverse (-)

9344096 .. 9350032

Gene Structure 5937 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G009320.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2589 bp

ATGGCGGAACCGACGCCGTCGGAGCAGATAGTCTATCTGCACGGCGACCTGGACCTTCAAATCATCGAGGCGCGCTACCTGCCCAACATGGACATCGTCTCCGAGCGCCTCCGCCGCTGCTTCACCGCCTGCGACACCGTCAACTGCACCAACTCCCGCTCCGACAATCCCGATGCCGGCCACGGAGCCGAGGACCGCAAGAAGGTCCACCACCGCAAAATCATCACCAGCGACTCTTACGTCAGCGTCGTTGTGCCCCAGGCCACGGTGGCGCGTACGCGCGTCATCAAGAACTCGCAGAATCCTCAGTGGAAAGAGACCTTTTCCATCCCGCTCGCGCATCCCGCGGCTAAGGTTGAGTTTCAGGTCAAAGATAACGACCTCTTCGGCGCTGAGCTTATCGGCACCGCCTCGATCCCCGCCGCAGAAGTCGCCTCCGGAAAATCCATTTCCGGCTGGTATGAGATTATCGGACCCACCGGAAAGCCTCCGAAGCCTGATTGCGCTATTAAGGTCGAGTTGAAGTTCACGCCCTTCGAGATAAACCCGGTCTACAGGCACGGCATCGCCGGCGATCCAGAACGGAAGGGAGTCCCCAAGACCTACTTCCCTATGAGGAAGGGAAGTCACGTGAGGCTGTACCAGGACGCGCACGTGCCGGAGGGGATGCTGCCGCAGATTGAATTGGACGGAAGGAAGGTTTATAAGCCTGAGAATTGCTGGGAGGACATTTGCTATGCGATTTCGGAGGCTCACCATATGATCTATGTGGTGGGATGGTCTGTGTTTCACAAAGTGAGGCTGGTTAGGGAGCCGAGCCGGAAGTTGCCACGCGGCGGCGATTTGACGCTCGGAGATTTGCTCAAGTATAAGTCGGAAGAAGGCGTACGGGTGTTGTTGCTGGTCTGGGATGATAAGACCTCCCACGACAAGTTCTTCTTCAAATCGGCAGGAATGATGCAAACTCATGATGAAGAAACCCGGAAGTTTTTCAAGCATTCGTCTGTCACTTGTGTTCTGGCCCCTCGCTATGGCAGTAGTAAGCTTAGCATTATGAAACAACAGGTTGTTGGAACCCTCTTTACACACCATCAAAAATGTGTTATTGTGGACACACAAGCATCTGGTAATAACCGCAAGATAACTACATTTTTAGGAGGTCTTGATCTCTGTGATGGTCGTTATGACACACCTGAGCACAGATTGTTTCGGGATCTCGACACTGTGTTTAAAGAAGATTTTCATCAACCTACGTTTCCTGCTGGGACCAAGGCTCCAAGGCAACCATGGCATGACTTGCACTGTAGAATTGACGGGCCTGCTGCATATGATGTTCTTATAAACTTTGAGCAACGGTGGAGGAGAGCAACACAGTGGAGGGAGTTTGGACTACGTAAAAAGGTTTCGCATTGGCATGATGATGCTTTAATTAAGATAGACCGTATTTCATGGATACTTAGTCCTAAGATGTCTCATTCAAAGGAGTCAAAGGATGTTACAATTGTTCCCGAAGATGACCCAGCTTTATGGGTTCAGGGAGAAGATGATCCCGAAGATTGGCATGTTCAGATTTTCAGGTCTATTGACTCGGGATCACTGAAAGGATTTCCCAAAGATGTCATTCATGCTCAGTCAAAGAACCTTATTTGCTCAAAGGATCTAGTAATAGATAAAAGCATCCAGAAAGCATACATCAATGCAATCAGATCTGCCCAACATTTCATATATATTGAAAATCAGTATTTTCTTGGTTCATCATATGCATGGCCAGATTATAAAAATGCAGGAGCTGACAATCTTATCCCAATGGAGTTGGCACTAAAGATTGCCAGCAAAATTAGAGCTAATGAGAGATTTGCAGTGTATATAGTTCTACCTATGTGGCCTGAAGGTGATCCGAAAACTGGTGCAATGCAAGAAATTCTCTACTGGCAGAGCCAAACAATGCAAATGATGTATGGTGTTGTTGCACGGGAATTGAGGGCCCTGCAGCTTCAGGACTTACATCCTCAAGATTTCCTCAATTTCTATTGCCTTGGTAATCGGGAAAAATTTTCTGAGGAAACATCAGACAACAATGGTGCTACGATCTCCGATGCATACAAGTACCAGCGATTTATGATTTATGTTCATGCCAAGGGTATGATAGTAGATGATGAGTACGTTATAGTGGGATCTGCCAATATCAACCAAAGATCATTGGCCGGTACGAAAGACACTGAGATAGCAATGGGTGCTTATCAACCCCATCACAGTTGGGCTGAGAAGAAGAGACATCCATGTGGTCAGATATATGGGTATCGAATGTCGCTCTGGGCTGAGCATCTTGGTAAGTCGGACCCATGCTTCAAAGAGGCAGAGAGTTTGGAATGTGTGAGGAATGTGAATGGCATTGCCGAAGAAAACTGGAGGAGATACACATTGCCGGATTTCACAGAACAACTGCAGGGTCACCTTCTCAGGTATCCGTTGCAGGTAGATGGTGATGGTAAGGTGCGACCCCTGCCAGGACATGAAAATTTCCCTGATGTTGGTGGCAAAGTTATTGGAGCTCATTCTGCTACACTTCCTGATCAATTGACAACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006950 GO:0008081 GO:0008150 GO:0008152 GO:0008219 GO:0009266 GO:0009409 GO:0009506 GO:0009628 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010119 GO:0010646 GO:0010647 GO:0012501 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019637 GO:0023051 GO:0023056 GO:0030054 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046473 GO:0046486 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0090333 GO:1901419 GO:1901421 GO:1905957 GO:1905959

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

862

Amino Acids

98.21

Weight (kDa)

6.84

Isoelectric Point (pI)

33.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	75 - 155	3.8e-16	C2 domain
PLDc_2	PF13091	701 - 744	1.2e-06	PLD-like domain
PLDc	PF00614	708 - 733	3.2e-08	Phospholipase D Active site motif
PLD_C	PF12357	781 - 851	1.6e-27	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0000855)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35790 AT4G35790 AT4G35790
fragaria_vesca	FvH4_1g11000 FvH4_1g11020 FvH4_1g11020 FvH4_2g37900 FvH4_5g01272 FvH4_5g28780 FvH4_5g28800 FvH4_7g08510 FvH4_7g08510
malus_domestica	MD02G1123100.v1.1 MD08G1149700.v1.1 MD08G1237200.v1.1 MD15G1006400.v1.1 MD15G1237100.v1.1
prunus_persica	Prupe.1G359000_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.7G175400_v2.0.a1
pyrus_communis	pycom02g09600 pycom15g00480 pycom15g21090
rosa_chinensis	RchiOBHm_Chr2g0098271 RchiOBHm_Chr6g0303711 RchiOBHm_Chr7g0221001 RchiOBHm_Chr7g0221011
rosa_laevigata	RLG00000002185 RLG00000011053 RLG00000016781
rosa_multiflora	Rmu_co8414259.1_g000001 Rmu_co8436567.1_g000002 Rmu_co8452585.1_g000001 Rmu_sc0001024.1_g000003 Rmu_sc0002105.1_g000010 Rmu_sc0002105.1_g000018 Rmu_sc0002105.1_g000020 Rmu_sc0009922.1_g000005 Rmu_sc0039265.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144090 Rroxscaffold_3G00238820 Rroxscaffold_7G00164460
rosa_rugosa	Rorug02G0071800 Rorug06G0324200 Rorug07G0199200 Rorug07G0199300 Rorug07G0199400 Rorug07G0199500 Rorug07G0199600
rosa_samantha	Rh1CG003000 Rh2AG118900 Rh2BG121800 Rh2CG123400 Rh2DG123500 Rh6AG438500 Rh6BG464500 Rh6CG450800 Rh6DG437500 Rh7AG339200 Rh7BG329900 Rh7CG357200 Rh7DG337900
rosa_wichuraiana	Rw2G009320 Rw6G037950 Rw7G028850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	705, 1340, 1776
AasI	GACNNNNNNGTC	1 cut(s)	239
Acc36I	ACCTGC	2 cut(s)	87, 2464
AccB1I	GGYRCC	1 cut(s)	404
AccBSI	CCGCTC	2 cut(s)	159, 334
AccI	GTMKAC	1 cut(s)	552
AccII	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
AccIII	TCCGGA	1 cut(s)	437
AclWI	GGATC	8 cut(s)	409, 569, 1221, 1541, 1600, 1665, 1889, 2182
AcoI	YGGCCR	3 cut(s)	178, 1766, 2202
AcsI	RAATTY	3 cut(s)	1920, 2051, 2518
AcuI	CTGAAG	2 cut(s)	1908, 1979
AcvI	CACGTG	2 cut(s)	631, 655
AcyI	GRCGYC	1 cut(s)	14
AdeI	CACNNNGTG	1 cut(s)	796
AfaI	GTAC	6 cut(s)	277, 641, 888, 2108, 2162, 2209
AfiI	CCNNNNNNNGG	9 cut(s)	176, 202, 265, 836, 1031, 1557, 1904, 2507, 2531
AgsI	TTSAA	8 cut(s)	59, 523, 683, 943, 994, 1479, 1733, 2350
AjnI	CCWGG	4 cut(s)	48, 258, 642, 2506
AjuI	GAANNNNNNNTTGG	2 cut(s)	590, 622
AluBI	AGCT	7 cut(s)	397, 1045, 1523, 1790, 1844, 1993, 2552
AluI	AGCT	7 cut(s)	397, 1045, 1523, 1790, 1844, 1993, 2552
Alw21I	GWGCWC	2 cut(s)	1200, 2554
Alw26I	GTCTC	4 cut(s)	103, 311, 1476, 2266
AlwI	GGATC	8 cut(s)	409, 569, 1221, 1541, 1600, 1665, 1889, 2182
AlwNI	CAGNNNCTG	1 cut(s)	1790
Ama87I	CYCGRG	1 cut(s)	1588
Aor13HI	TCCGGA	1 cut(s)	437
AoxI	GGCC	8 cut(s)	178, 261, 1020, 1316, 1766, 1883, 1983, 2202
ApaI	GGGCCC	1 cut(s)	1987
ApeKI	GCWGC	4 cut(s)	117, 670, 1322, 1990
ApoI	RAATTY	3 cut(s)	1920, 2051, 2518
Asp700I	GAANNNNTTC	2 cut(s)	989, 1239
AspLEI	GCGC	9 cut(s)	73, 75, 108, 274, 282, 340, 392, 506, 652
AsuC2I	CCSGG	2 cut(s)	548, 982
AsuHPI	GGTGA	6 cut(s)	115, 217, 746, 1904, 2444, 2495
AvaI	CYCGRG	1 cut(s)	1588
AvaII	GGWCC	6 cut(s)	52, 193, 205, 473, 1266, 2338
BaeGI	GKGCMC	2 cut(s)	258, 1987
BalI	TGGCCA	1 cut(s)	1768
BanI	GGYRCC	1 cut(s)	404
BanII	GRGCYC	2 cut(s)	1987, 2554
BarI	GAAGNNNNNNTAC	2 cut(s)	870, 902
BbrPI	CACGTG	2 cut(s)	631, 655
Bbv12I	GWGCWC	2 cut(s)	1200, 2554
BbvI	GCAGC	4 cut(s)	104, 657, 1309, 2002
BccI	CCATC	7 cut(s)	335, 771, 1098, 1169, 2256, 2474, 2480
BceAI	ACGGC	2 cut(s)	58, 577
BciT130I	CCWGG	4 cut(s)	50, 260, 644, 2508
BciVI	GTATCC	2 cut(s)	1446, 2475
BclI	TGATCA	1 cut(s)	2572
BcnI	CCSGG	2 cut(s)	548, 982
BcoDI	GTCTC	4 cut(s)	103, 311, 1476, 2266
BfaI	CTAG	1 cut(s)	1661
BfmI	CTRYAG	4 cut(s)	553, 1303, 1988, 2444
BfoI	RGCGCY	2 cut(s)	109, 393
BfuAI	ACCTGC	2 cut(s)	87, 2464
BfuI	GTATCC	2 cut(s)	1446, 2475
BglI	GCCNNNNNGGC	1 cut(s)	269
BglII	AGATCT	1 cut(s)	1706
BlpI	GCTNAGC	3 cut(s)	393, 1046, 2319
Bme1390I	CCNGG	6 cut(s)	50, 260, 548, 644, 982, 2508
Bme18I	GGWCC	6 cut(s)	52, 193, 205, 473, 1266, 2338
BmeT110I	CYCGRG	1 cut(s)	1588
BmrFI	CCNGG	6 cut(s)	50, 260, 548, 644, 982, 2508
BplI	GAGNNNNNCTC	2 cut(s)	846, 878
BpmI	CTGGAG	1 cut(s)	2428
Bpu10I	CCTNAGC	2 cut(s)	351, 1194
Bpu1102I	GCTNAGC	3 cut(s)	393, 1046, 2319
BpuEI	CTTGAG	2 cut(s)	848, 1995
BpuMI	CCSGG	2 cut(s)	548, 982
BsaAI	YACGTR	3 cut(s)	631, 655, 1394
BsaBI	GATNNNNATC	1 cut(s)	1242
BsaHI	GRCGYC	1 cut(s)	14
BsaI	GGTCTC	1 cut(s)	311
BsaWI	WCCGGW	2 cut(s)	437, 479
BsaXI	ACNNNNNCTCC	6 cut(s)	83, 113, 143, 173, 1258, 1288
Bsc4I	CCNNNNNNNGG	9 cut(s)	176, 202, 265, 836, 1031, 1557, 1904, 2507, 2531
Bse118I	RCCGGY	3 cut(s)	176, 569, 2204
Bse1I	ACTGG	3 cut(s)	1909, 1934, 2411
Bse3DI	GCAATG	4 cut(s)	1917, 2235, 2391, 2420
Bse8I	GATNNNNATC	1 cut(s)	1242
BseAI	TCCGGA	1 cut(s)	437
BseBI	CCWGG	4 cut(s)	50, 260, 644, 2508
BseGI	GGATG	9 cut(s)	327, 340, 672, 782, 916, 1498, 1677, 2005, 2275
BseJI	GATNNNNATC	1 cut(s)	1242
BseLI	CCNNNNNNNGG	9 cut(s)	176, 202, 265, 836, 1031, 1557, 1904, 2507, 2531
BseMI	GCAATG	4 cut(s)	1917, 2235, 2391, 2420
BseNI	ACTGG	3 cut(s)	1909, 1934, 2411
BsePI	GCGCGC	1 cut(s)	71
BseRI	GAGGAG	2 cut(s)	1375, 2425
BseSI	GKGCMC	2 cut(s)	258, 1987
BseXI	GCAGC	4 cut(s)	104, 657, 1309, 2002
BseYI	CCCAGC	3 cut(s)	720, 1262, 1519
BsgI	GTGCAG	2 cut(s)	23, 130
Bsh1236I	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BshFI	GGCC	8 cut(s)	180, 263, 1022, 1318, 1768, 1885, 1985, 2204
BshNI	GGYRCC	1 cut(s)	404
BsiHKAI	GWGCWC	2 cut(s)	1200, 2554
BsiHKCI	CYCGRG	1 cut(s)	1588
BsiWI	CGTACG	2 cut(s)	275, 886
BslFI	GGGAC	2 cut(s)	578, 1279
BslI	CCNNNNNNNGG	9 cut(s)	176, 202, 265, 836, 1031, 1557, 1904, 2507, 2531
BsmAI	GTCTC	4 cut(s)	103, 311, 1476, 2266
BsmBI	CGTCTC	1 cut(s)	103
BsmFI	GGGAC	2 cut(s)	578, 1279
BsmI	GAATGC	1 cut(s)	997
BsnI	GGCC	8 cut(s)	180, 263, 1022, 1318, 1768, 1885, 1985, 2204
Bso31I	GGTCTC	1 cut(s)	311
BsoBI	CYCGRG	1 cut(s)	1588
Bsp120I	GGGCCC	1 cut(s)	1983
Bsp1286I	GDGCHC	4 cut(s)	258, 1200, 1987, 2554
Bsp13I	TCCGGA	1 cut(s)	437
Bsp1720I	GCTNAGC	3 cut(s)	393, 1046, 2319
Bsp19I	CCATGG	1 cut(s)	1286
BspANI	GGCC	8 cut(s)	180, 263, 1022, 1318, 1768, 1885, 1985, 2204
BspEI	TCCGGA	1 cut(s)	437
BspFNI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BspHI	TCATGA	1 cut(s)	967
BspMAI	CTGCAG	2 cut(s)	1992, 2448
BspMI	ACCTGC	2 cut(s)	87, 2464
BspPI	GGATC	8 cut(s)	409, 569, 1221, 1541, 1600, 1665, 1889, 2182
BspT107I	GGYRCC	1 cut(s)	404
BspTNI	GGTCTC	1 cut(s)	311
BsrBI	CCGCTC	2 cut(s)	159, 334
BsrDI	GCAATG	4 cut(s)	1917, 2235, 2391, 2420
BsrFI	RCCGGY	3 cut(s)	176, 569, 2204
BsrI	ACTGG	3 cut(s)	1909, 1934, 2411
BssAI	RCCGGY	3 cut(s)	176, 569, 2204
BssHII	GCGCGC	1 cut(s)	71
BssNI	GRCGYC	1 cut(s)	14
BssT1I	CCWWGG	5 cut(s)	1269, 1277, 1286, 2035, 2136
Bst2UI	CCWGG	4 cut(s)	50, 260, 644, 2508
Bst4CI	ACNGT	8 cut(s)	139, 268, 1225, 1304, 1356, 1374, 1442, 2255
Bst6I	CTCTTC	2 cut(s)	389, 2264
BstACI	GRCGYC	1 cut(s)	14
BstAPI	GCANNNNNTGC	2 cut(s)	1297, 2234
BstBAI	YACGTR	3 cut(s)	631, 655, 1394
BstC8I	GCNNGC	7 cut(s)	73, 130, 178, 336, 571, 1320, 1831
BstDSI	CCRYGG	4 cut(s)	181, 264, 345, 1286
BstEII	GGTNACC	1 cut(s)	2450
BstF5I	GGATG	9 cut(s)	327, 340, 672, 782, 916, 1498, 1677, 2005, 2275
BstFNI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BstH2I	RGCGCY	2 cut(s)	109, 393
BstHHI	GCGC	9 cut(s)	73, 75, 108, 274, 282, 340, 392, 506, 652
BstMAI	GTCTC	4 cut(s)	103, 311, 1476, 2266
BstNI	CCWGG	4 cut(s)	50, 260, 644, 2508
BstNSI	RCATGY	1 cut(s)	1565
BstPI	GGTNACC	1 cut(s)	2450
BstSCI	CCNGG	6 cut(s)	48, 258, 546, 642, 980, 2506
BstSFI	CTRYAG	4 cut(s)	553, 1303, 1988, 2444
BstSLI	GKGCMC	2 cut(s)	258, 1987
BstSNI	TACGTA	1 cut(s)	1394
BstUI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BstV1I	GCAGC	4 cut(s)	104, 657, 1309, 2002
BstX2I	RGATCY	4 cut(s)	1213, 1657, 1706, 2174
BstXI	CCANNNNNNTGG	1 cut(s)	1527
BstYI	RGATCY	4 cut(s)	1213, 1657, 1706, 2174
BsuI	GTATCC	2 cut(s)	1446, 2475
BsuRI	GGCC	8 cut(s)	180, 263, 1022, 1318, 1768, 1885, 1985, 2204
BtgI	CCRYGG	4 cut(s)	181, 264, 345, 1286
BtgZI	GCGATG	1 cut(s)	550
BtsCI	GGATG	9 cut(s)	327, 340, 672, 782, 916, 1498, 1677, 2005, 2275
BtsI	GCAGTG	1 cut(s)	1866
BtsIMutI	CAGTG	7 cut(s)	314, 1221, 1300, 1379, 1595, 1866, 2217
BveI	ACCTGC	2 cut(s)	87, 2464
Cac8I	GCNNGC	7 cut(s)	73, 130, 178, 336, 571, 1320, 1831
CaiI	CAGNNNCTG	1 cut(s)	1790
CciI	TCATGA	1 cut(s)	967
CfoI	GCGC	9 cut(s)	73, 75, 108, 274, 282, 340, 392, 506, 652
Cfr10I	RCCGGY	3 cut(s)	176, 569, 2204
Cfr42I	CCGCGG	1 cut(s)	348
CseI	GACGC	5 cut(s)	22, 235, 271, 656, 856
Csp6I	GTAC	6 cut(s)	276, 640, 887, 2107, 2161, 2208
CviQI	GTAC	6 cut(s)	276, 640, 887, 2107, 2161, 2208
DraI	TTTAAA	1 cut(s)	1231
DraIII	CACNNNGTG	1 cut(s)	796
DrdI	GACNNNNNNGTC	1 cut(s)	239
DseDI	GACNNNNNNGTC	1 cut(s)	239
EaeI	YGGCCR	3 cut(s)	178, 1766, 2202
Eam1104I	CTCTTC	2 cut(s)	389, 2264
EarI	CTCTTC	2 cut(s)	389, 2264
EciI	GGCGGA	2 cut(s)	20, 101
Ecl136II	GAGCTC	1 cut(s)	2552
Eco105I	TACGTA	1 cut(s)	1394
Eco130I	CCWWGG	5 cut(s)	1269, 1277, 1286, 2035, 2136
Eco24I	GRGCYC	2 cut(s)	1987, 2554
Eco31I	GGTCTC	1 cut(s)	311
Eco47I	GGWCC	6 cut(s)	52, 193, 205, 473, 1266, 2338
Eco53kI	GAGCTC	1 cut(s)	2552
Eco57I	CTGAAG	2 cut(s)	1908, 1979
Eco72I	CACGTG	2 cut(s)	631, 655
Eco88I	CYCGRG	1 cut(s)	1588
Eco91I	GGTNACC	1 cut(s)	2450
EcoICRI	GAGCTC	1 cut(s)	2552
EcoO109I	RGGNCCY	2 cut(s)	1983, 1984
EcoO65I	GGTNACC	1 cut(s)	2450
EcoRII	CCWGG	4 cut(s)	48, 258, 642, 2506
EcoT14I	CCWWGG	5 cut(s)	1269, 1277, 1286, 2035, 2136
EcoT22I	ATGCAT	2 cut(s)	1765, 2101
EcoT38I	GRGCYC	2 cut(s)	1987, 2554
ErhI	CCWWGG	5 cut(s)	1269, 1277, 1286, 2035, 2136
Esp3I	CGTCTC	1 cut(s)	103
FalI	AAGNNNNNCTT	4 cut(s)	590, 622, 923, 955
FaqI	GGGAC	2 cut(s)	578, 1279
FauI	CCCGC	4 cut(s)	164, 339, 352, 427
FauNDI	CATATG	3 cut(s)	759, 1327, 1759
FbaI	TGATCA	1 cut(s)	2572
FblI	GTMKAC	1 cut(s)	552
FokI	GGATG	9 cut(s)	314, 327, 679, 789, 923, 1505, 1664, 1992, 2262
FriOI	GRGCYC	2 cut(s)	1987, 2554
FspBI	CTAG	1 cut(s)	1661
GlaI	GCGC	9 cut(s)	72, 74, 107, 273, 281, 339, 391, 505, 651
GsaI	CCCAGC	3 cut(s)	724, 1266, 1523
GsuI	CTGGAG	1 cut(s)	2428
HaeII	RGCGCY	2 cut(s)	109, 393
HaeIII	GGCC	8 cut(s)	180, 263, 1022, 1318, 1768, 1885, 1985, 2204
HgaI	GACGC	5 cut(s)	22, 235, 271, 656, 856
HhaI	GCGC	9 cut(s)	73, 75, 108, 274, 282, 340, 392, 506, 652
Hin1I	GRCGYC	1 cut(s)	14
Hin6I	GCGC	9 cut(s)	71, 73, 106, 272, 280, 338, 390, 504, 650
HinP1I	GCGC	9 cut(s)	71, 73, 106, 272, 280, 338, 390, 504, 650
HincII	GTYRAC	1 cut(s)	142
HindII	GTYRAC	1 cut(s)	142
HindIII	AAGCTT	1 cut(s)	1043
HinfI	GANTC	5 cut(s)	233, 301, 591, 1484, 1586
HphI	GGTGA	6 cut(s)	115, 217, 746, 1904, 2444, 2495
Hpy166II	GTNNAC	5 cut(s)	142, 208, 528, 553, 1111
Hpy8I	GTNNAC	5 cut(s)	142, 208, 528, 553, 1111
Hpy99I	CGWCG	3 cut(s)	16, 22, 251
HpyCH4III	ACNGT	8 cut(s)	139, 268, 1225, 1304, 1356, 1374, 1442, 2255
HpyCH4IV	ACGT	6 cut(s)	240, 630, 654, 1253, 1393, 2163
HpySE526I	ACGT	6 cut(s)	240, 630, 654, 1253, 1393, 2163
Hsp92I	GRCGYC	1 cut(s)	14
HspAI	GCGC	9 cut(s)	71, 73, 106, 272, 280, 338, 390, 504, 650
Kpn2I	TCCGGA	1 cut(s)	437
KroI	GCCGGC	2 cut(s)	176, 569
KroNI	GCCGGC	2 cut(s)	178, 571
Ksp22I	TGATCA	1 cut(s)	2572
KspI	CCGCGG	1 cut(s)	348
LmnI	GCTCC	7 cut(s)	22, 164, 185, 811, 1279, 1787, 2549
Lsp1109I	GCAGC	4 cut(s)	104, 657, 1309, 2002
MaeI	CTAG	1 cut(s)	1661
MaeII	ACGT	6 cut(s)	240, 630, 654, 1253, 1393, 2163
MaeIII	GTNAC	4 cut(s)	626, 1006, 1495, 2450
MbiI	CCGCTC	2 cut(s)	159, 334
MfeI	CAATTG	2 cut(s)	1500, 2576
MflI	RGATCY	4 cut(s)	1213, 1657, 1706, 2174
MhlI	GDGCHC	4 cut(s)	258, 1200, 1987, 2554
MlsI	TGGCCA	1 cut(s)	1768
MluNI	TGGCCA	1 cut(s)	1768
MlyI	GAGTC	4 cut(s)	227, 600, 1493, 1580
MmeI	TCCRAC	4 cut(s)	186, 855, 1051, 2316
Mox20I	TGGCCA	1 cut(s)	1768
Mph1103I	ATGCAT	2 cut(s)	1765, 2101
MreI	CGCCGGCG	1 cut(s)	569
MroI	TCCGGA	1 cut(s)	437
MroNI	GCCGGC	2 cut(s)	176, 569
MroXI	GAANNNNTTC	2 cut(s)	989, 1239
MscI	TGGCCA	1 cut(s)	1768
MseI	TTAA	4 cut(s)	510, 1230, 1427, 1431
MslI	CAYNNNNRTG	4 cut(s)	1413, 1419, 1762, 2280
Msp20I	TGGCCA	1 cut(s)	1768
MspA1I	CMGCKG	2 cut(s)	117, 347
MspR9I	CCNGG	6 cut(s)	50, 260, 548, 644, 982, 2508
MunI	CAATTG	2 cut(s)	1500, 2576
Mva1269I	GAATGC	1 cut(s)	997
MvaI	CCWGG	4 cut(s)	50, 260, 644, 2508
MvnI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
NaeI	GCCGGC	2 cut(s)	178, 571
NciI	CCSGG	2 cut(s)	548, 982
NcoI	CCATGG	1 cut(s)	1286
NdeI	CATATG	3 cut(s)	759, 1327, 1759
NgoMIV	GCCGGC	2 cut(s)	176, 569
NmeAIII	GCCGAG	2 cut(s)	214, 840
NmuCI	GTSAC	3 cut(s)	626, 1006, 2450
NsiI	ATGCAT	2 cut(s)	1765, 2101
NspI	RCATGY	1 cut(s)	1565
PacI	TTAATTAA	1 cut(s)	1431
PagI	TCATGA	1 cut(s)	967
PauI	GCGCGC	1 cut(s)	71
PcsI	WCGNNNNNNNCGW	1 cut(s)	246
PctI	GAATGC	1 cut(s)	997
PdiI	GCCGGC	2 cut(s)	178, 571
PdmI	GAANNNNTTC	2 cut(s)	989, 1239
PfeI	GAWTC	1 cut(s)	301
Pfl23II	CGTACG	2 cut(s)	275, 886
PflFI	GACNNNGTC	3 cut(s)	16, 95, 137
PleI	GAGTC	4 cut(s)	227, 599, 1492, 1580
PmaCI	CACGTG	2 cut(s)	631, 655
PmlI	CACGTG	2 cut(s)	631, 655
PpsI	GAGTC	4 cut(s)	227, 599, 1492, 1580
Ppu21I	YACGTR	3 cut(s)	631, 655, 1394
PsiI	TTATAA	3 cut(s)	705, 1340, 1776
Psp124BI	GAGCTC	1 cut(s)	2554
Psp6I	CCWGG	4 cut(s)	48, 258, 642, 2506
PspCI	CACGTG	2 cut(s)	631, 655
PspEI	GGTNACC	1 cut(s)	2450
PspFI	CCCAGC	3 cut(s)	720, 1262, 1519
PspGI	CCWGG	4 cut(s)	48, 258, 642, 2506
PspLI	CGTACG	2 cut(s)	275, 886
PspOMI	GGGCCC	1 cut(s)	1983
PsrI	GAACNNNNNNTAC	2 cut(s)	1066, 1098
PstI	CTGCAG	2 cut(s)	1992, 2448
PstNI	CAGNNNCTG	1 cut(s)	1790
PsuI	RGATCY	4 cut(s)	1213, 1657, 1706, 2174
PsyI	GACNNNGTC	3 cut(s)	16, 95, 137
PteI	GCGCGC	1 cut(s)	71
RsaI	GTAC	6 cut(s)	277, 641, 888, 2108, 2162, 2209
RsaNI	GTAC	6 cut(s)	276, 640, 887, 2107, 2161, 2208
RseI	CAYNNNNRTG	4 cut(s)	1413, 1419, 1762, 2280
SacI	GAGCTC	1 cut(s)	2554
SacII	CCGCGG	1 cut(s)	348
SaqAI	TTAA	4 cut(s)	510, 1230, 1427, 1431
SchI	GAGTC	4 cut(s)	227, 600, 1493, 1580
ScrFI	CCNGG	6 cut(s)	50, 260, 548, 644, 982, 2508
SduI	GDGCHC	4 cut(s)	258, 1200, 1987, 2554
SfcI	CTRYAG	4 cut(s)	553, 1303, 1988, 2444
Sfr303I	CCGCGG	1 cut(s)	348
SgrAI	CRCCGGYG	1 cut(s)	569
SgrBI	CCGCGG	1 cut(s)	348
SinI	GGWCC	6 cut(s)	52, 193, 205, 473, 1266, 2338
SmiMI	CAYNNNNRTG	4 cut(s)	1413, 1419, 1762, 2280
SmlI	CTYRAG	2 cut(s)	863, 2010
SmoI	CTYRAG	2 cut(s)	863, 2010
SnaBI	TACGTA	1 cut(s)	1394
SspMI	CTAG	1 cut(s)	1661
SstI	GAGCTC	1 cut(s)	2554
StyD4I	CCNGG	6 cut(s)	48, 258, 546, 642, 980, 2506
StyI	CCWWGG	5 cut(s)	1269, 1277, 1286, 2035, 2136
TaaI	ACNGT	8 cut(s)	139, 268, 1225, 1304, 1356, 1374, 1442, 2255
TaiI	ACGT	6 cut(s)	243, 633, 657, 1256, 1396, 2166
TaqI	TCGA	6 cut(s)	66, 413, 516, 537, 1218, 2302
TauI	GCSGC	6 cut(s)	117, 350, 425, 676, 837, 840
TfiI	GAWTC	1 cut(s)	301
Tru1I	TTAA	4 cut(s)	510, 1230, 1427, 1431
Tru9I	TTAA	4 cut(s)	510, 1230, 1427, 1431
TscAI	CASTG	7 cut(s)	314, 1228, 1307, 1379, 1602, 1866, 2224
TseFI	GTSAC	3 cut(s)	626, 1006, 2450
TseI	GCWGC	4 cut(s)	117, 670, 1322, 1990
Tsp45I	GTSAC	3 cut(s)	626, 1006, 2450
TspDTI	ATGAA	9 cut(s)	987, 1070, 1232, 1437, 1613, 1711, 1743, 2120, 2529
TspGWI	ACGGA	4 cut(s)	198, 598, 705, 2457
TspRI	CASTG	7 cut(s)	314, 1228, 1307, 1379, 1602, 1866, 2224
Tth111I	GACNNNGTC	3 cut(s)	16, 95, 137
VpaK11BI	GGWCC	6 cut(s)	52, 193, 205, 473, 1266, 2338
XapI	RAATTY	3 cut(s)	1920, 2051, 2518
XceI	RCATGY	1 cut(s)	1565
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1284
XmiI	GTMKAC	1 cut(s)	552
XmnI	GAANNNNTTC	2 cut(s)	989, 1239
XspI	CTAG	1 cut(s)	1661
Zsp2I	ATGCAT	2 cut(s)	1765, 2101

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.