Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000855

Physical Location & Seq

Genomic Coordinates Reverse (-)

10664746 .. 10670689

Gene Structure 5944 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ47307

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2577 bp

ATGGCGGAACCGACGTCGTCGGAGCAGATAGTCTATCTGCACGGCGACCTGGACCTTCAAATCATCGAGGCGCGCTACCTGCCCAACATGGACATCGTCTCCGAGCGCCTCCGCCGCTGCTTCACCGCCTGCGACACCGTCAACTGCACCGGCTCCCGCTCCGACAATCCAGATGCCGGCCACGGAGCCGAGGACCGCAAGAAGCTCCACCACCGCAAAATCATCACCAGCGACTCTTACGTCAGCGTCGTTGTGCCCCAGGCCACGGTAGCGCGTACGCGCGTCATCAAGAACTCGCAGAATCCTCTGTGGAAAGAGTCCTTTTCCATCCCGCTCGCGCATCCCGCGGCTAAGCTTGAGTTTCAGGTCAAAGATAACGACCTCTTCGGCGCTGAGCTTATCGGCACCGCCTCCATCCCCGCCGTCGAAATCGCCGCCGGAAATGAAATTTCCGGCTGGTATGAGATTATCGGACCCACCGGAAAGCCTCCGAAGCCTGATTGCGCTATCAAGGTCAAGTTGAAGTTCACGCCCTTCGAGATAAACCCGGTCTACAGGCACGGCATCGCCGGCGATCCAGAACGGAAGGGAGTCCCCAAGACCTACTTTCCTATGAGGAAGGGAAGTCACGTGAGGCTGTACCAGGACGCGCACGTGCCGGAGGGGATGCTGCCGCAGATTGAATTGGACGGAAGGAAGGTTTATAAGCCTGAGAATTGCTGGGAGGACATTTGCTATGCGATTTCGGAGGCTCACCATATGATCTATGTGGTGGGATGGTCTGTGTTTCACAAAGTGAGGCTGGTTAGGGAGCCGAGCAGGAAGTTGCCACGCGGCGGCGAGTTGACGCTCGGAGAGTTGCTCAAGTATAAATCGGAAGAAGGCGTACGGGTTTTGTTGCTGGTCTGGGATGATAAGACCTCCCACGACAAGTTCTTCTTCAAATCGGCGGGAATGATGCAAACTCATGATGAAGAAACCCGGAAGTTTTTCAAGCATTCGTCTGTCACTTGTGTTCTGGCCCCTCGCTATGGCAGTAGTAAGCTTAGCATTATGAAACAACAGGTTGTTGGAACCCTCTTCACACACCATCAAAAATGTGTTATTGTGGACACACAAGCATCTGGTAATAACCGCAAGATAACTACATTTTTAGGAGGTCTTGATCTCTGTGATGGTCGTTATGACACACCTGAGCACAGATTGTTTCGGGATCTCGACACTGTGTTTAAAGAAGATTTTCATCAACCTACGTTTCCTGCTGGGACCAAGGCTCCAAGGCAACCATGGCATGACTTGCACTGTAGAATTGACGGGCCTGCTGCATATGATGTTCTTATAAACTTTGAGCAACGGTGGAGGAGAGCAACACAGTGGAGGGAGTTTGGACTGCGTAAAAAGGTTTCGCATTGGCATGATGATGCTTTAATTAAGATAGACCGTATTTCATGGATACTTAGTCCTAAGATGTCTCATTCAAAGGATGTTACAATTGTTCCTGAAGATGACCCAGCTTTATGGGTTCAGAGAGAAGATGATCCCGAAGATTGGCATGTTCAGATTTTCAGGTCTATTGACTCGGGATCACTGAAAGGATTTCCCAAAGATGTCATTCAGGCTCAGTCAAAGAACCTTATTTGCTCAAAGGATCTAGTAATAGATAAAAGCATCCAGAAAGCATACATCAATGCAATCAGATCTGCCCAACATTTCATATATATTGAAAATCAGTATTTTCTTGGTTCATCATATGCATGGCCAGATTATAAAAATGCAGGAGCTGACAATCTTATCCCAATGGAGTTGGCACTAAAGATTGCCAGCAAAATTAGAGCTAATGAGAGATTTGCAGTGTATATAGTTCTACCTATGTGGCCTGAAGGTGATCCGAAAACTGGTGCAATGCAAGAAATTCTCTACTGGCAGAGCCAAACAATGCAAATGATGTATGGTGTTGTTGCACGGGAATTGAGGGCCCTGCAGCTTCAAGACTTACATCCTCAAGATTTCCTCAATTTCTATTGCCTTGGTAATCGGGAAAAACTTTCTGAGGAAACATCAAACAACAATGGTGCTACGATCTCCGATGCATACAAGTACCAGCGCTTTATGATTTATGTTCATGCCAAGGGTATGATAGTAGATGACGAGTACGTTATAGTGGGATCTGCCAATATCAACCAAAGATCAATGGCCGGTACAAAAGACACTGAGATAGCTATGGGTGCGTATCAACCCCATCACAGTTGGTCTGAGAAGAAGAGACATCCATGTGGTCAGATATATGGGTATCGAATGTCGCTCTGGGCTGAGCATCTTGGTAAGTCGGACCCATGCTTCAAAGAGGCAGAGAGTTTGGAATGTGTGAGGAATGTGAATGGCATTGCCGAAGAAAACTGGAGGAGATACACATCGCCGGATTTCACAGAACTGCAGGGTCACCTTCTCAGGTATCCGTTGCAGGTAGATGGTGATGGTAACGTGCGACCCCGGCCAGGATATGAAAATTTCCCTGATGTTGGTGGCAAAGTTATTGGAGCTCATTCTGCCACACTTCCTGATCAATTGACAACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006950 GO:0008081 GO:0008150 GO:0008152 GO:0008219 GO:0009266 GO:0009409 GO:0009506 GO:0009628 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010119 GO:0010646 GO:0010647 GO:0012501 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019637 GO:0023051 GO:0023056 GO:0030054 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046473 GO:0046486 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0090333 GO:1901419 GO:1901421 GO:1905957 GO:1905959

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

858

Amino Acids

97.87

Weight (kDa)

7.05

Isoelectric Point (pI)

34.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	74 - 155	2.4e-15	C2 domain
PLDc_2	PF13091	698 - 741	1.2e-06	PLD-like domain
PLDc	PF00614	705 - 730	3.1e-08	Phospholipase D Active site motif
PLD_C	PF12357	778 - 847	1e-27	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000855)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35790 AT4G35790 AT4G35790
fragaria_vesca	FvH4_1g11000 FvH4_1g11020 FvH4_1g11020 FvH4_2g37900 FvH4_5g01272 FvH4_5g28780 FvH4_5g28800 FvH4_7g08510 FvH4_7g08510
malus_domestica	MD02G1123100.v1.1 MD08G1149700.v1.1 MD08G1237200.v1.1 MD15G1006400.v1.1 MD15G1237100.v1.1
prunus_persica	Prupe.1G359000_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.7G175400_v2.0.a1
pyrus_communis	pycom02g09600 pycom15g00480 pycom15g21090
rosa_chinensis	RchiOBHm_Chr2g0098271 RchiOBHm_Chr6g0303711 RchiOBHm_Chr7g0221001 RchiOBHm_Chr7g0221011
rosa_laevigata	RLG00000002185 RLG00000011053 RLG00000016781
rosa_multiflora	Rmu_co8414259.1_g000001 Rmu_co8436567.1_g000002 Rmu_co8452585.1_g000001 Rmu_sc0001024.1_g000003 Rmu_sc0002105.1_g000010 Rmu_sc0002105.1_g000018 Rmu_sc0002105.1_g000020 Rmu_sc0009922.1_g000005 Rmu_sc0039265.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144090 Rroxscaffold_3G00238820 Rroxscaffold_7G00164460
rosa_rugosa	Rorug02G0071800 Rorug06G0324200 Rorug07G0199200 Rorug07G0199300 Rorug07G0199400 Rorug07G0199500 Rorug07G0199600
rosa_samantha	Rh1CG003000 Rh2AG118900 Rh2BG121800 Rh2CG123400 Rh2DG123500 Rh6AG438500 Rh6BG464500 Rh6CG450800 Rh6DG437500 Rh7AG339200 Rh7BG329900 Rh7CG357200 Rh7DG337900
rosa_wichuraiana	Rw2G009320 Rw6G037950 Rw7G028850

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	705, 1340, 1767
AasI	GACNNNNNNGTC	1 cut(s)	239
AatII	GACGTC	1 cut(s)	17
Acc36I	ACCTGC	2 cut(s)	87, 2452
AccB1I	GGYRCC	1 cut(s)	404
AccBSI	CCGCTC	2 cut(s)	159, 334
AccI	GTMKAC	1 cut(s)	552
AccII	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
AclWI	GGATC	7 cut(s)	569, 1221, 1532, 1591, 1656, 1880, 2173
AcoI	YGGCCR	4 cut(s)	178, 1757, 2193, 2492
AcsI	RAATTY	3 cut(s)	447, 1911, 2506
AcuI	CTGAAG	2 cut(s)	1521, 1899
AcvI	CACGTG	2 cut(s)	631, 655
AcyI	GRCGYC	1 cut(s)	14
AdeI	CACNNNGTG	1 cut(s)	796
AfaI	GTAC	6 cut(s)	277, 641, 888, 2099, 2153, 2200
AfeI	AGCGCT	1 cut(s)	2105
AfiI	CCNNNNNNNGG	8 cut(s)	176, 265, 836, 1031, 1548, 1895, 2495, 2519
AgsI	TTSAA	9 cut(s)	59, 523, 683, 943, 994, 1479, 1724, 1988, 2341
AjnI	CCWGG	4 cut(s)	48, 258, 642, 2494
Alw21I	GWGCWC	2 cut(s)	1200, 2542
Alw26I	GTCTC	3 cut(s)	103, 1476, 2257
AlwI	GGATC	7 cut(s)	569, 1221, 1532, 1591, 1656, 1880, 2173
AlwNI	CAGNNNCTG	1 cut(s)	1781
Ama87I	CYCGRG	1 cut(s)	1579
Aor51HI	AGCGCT	1 cut(s)	2105
AoxI	GGCC	9 cut(s)	178, 261, 1020, 1316, 1757, 1874, 1974, 2193, 2492
ApaI	GGGCCC	1 cut(s)	1978
ApeKI	GCWGC	4 cut(s)	117, 670, 1322, 1981
ApoI	RAATTY	3 cut(s)	447, 1911, 2506
Asp700I	GAANNNNTTC	2 cut(s)	989, 1239
AspS9I	GGNCC	9 cut(s)	52, 193, 473, 1021, 1266, 1316, 1974, 1975, 2329
AsuC2I	CCSGG	3 cut(s)	548, 982, 2491
AsuHPI	GGTGA	6 cut(s)	115, 217, 746, 1895, 2432, 2483
AvaI	CYCGRG	1 cut(s)	1579
AvaII	GGWCC	5 cut(s)	52, 193, 473, 1266, 2329
BaeGI	GKGCMC	2 cut(s)	258, 1978
BalI	TGGCCA	1 cut(s)	1759
BanI	GGYRCC	1 cut(s)	404
BanII	GRGCYC	2 cut(s)	1978, 2542
BarI	GAAGNNNNNNTAC	2 cut(s)	870, 902
BbrPI	CACGTG	2 cut(s)	631, 655
Bbv12I	GWGCWC	2 cut(s)	1200, 2542
BbvI	GCAGC	4 cut(s)	104, 657, 1309, 1993
BccI	CCATC	8 cut(s)	335, 422, 771, 1098, 1169, 2247, 2462, 2468
BceAI	ACGGC	3 cut(s)	58, 407, 577
BciT130I	CCWGG	4 cut(s)	50, 260, 644, 2496
BciVI	GTATCC	2 cut(s)	1446, 2463
BclI	TGATCA	1 cut(s)	2560
BcnI	CCSGG	3 cut(s)	548, 982, 2491
BcoDI	GTCTC	3 cut(s)	103, 1476, 2257
BfaI	CTAG	1 cut(s)	1652
BfmI	CTRYAG	4 cut(s)	553, 1303, 1979, 2432
BfoI	RGCGCY	3 cut(s)	109, 393, 2107
BfuAI	ACCTGC	2 cut(s)	87, 2452
BfuI	GTATCC	2 cut(s)	1446, 2463
BglII	AGATCT	1 cut(s)	1697
BlpI	GCTNAGC	4 cut(s)	351, 393, 1046, 2310
Bme1390I	CCNGG	7 cut(s)	50, 260, 548, 644, 982, 2491, 2496
Bme18I	GGWCC	5 cut(s)	52, 193, 473, 1266, 2329
BmeT110I	CYCGRG	1 cut(s)	1579
BmgT120I	GGNCC	9 cut(s)	52, 193, 473, 1021, 1266, 1316, 1974, 1975, 2329
BmrFI	CCNGG	7 cut(s)	50, 260, 548, 644, 982, 2491, 2496
BplI	GAGNNNNNCTC	2 cut(s)	846, 878
BpmI	CTGGAG	1 cut(s)	2419
Bpu10I	CCTNAGC	1 cut(s)	1194
Bpu1102I	GCTNAGC	4 cut(s)	351, 393, 1046, 2310
BpuEI	CTTGAG	3 cut(s)	377, 848, 1986
BpuMI	CCSGG	3 cut(s)	548, 982, 2491
BsaAI	YACGTR	2 cut(s)	631, 655
BsaBI	GATNNNNATC	2 cut(s)	1242, 2410
BsaHI	GRCGYC	1 cut(s)	14
BsaWI	WCCGGW	1 cut(s)	479
BsaXI	ACNNNNNCTCC	4 cut(s)	83, 113, 1258, 1288
Bsc4I	CCNNNNNNNGG	8 cut(s)	176, 265, 836, 1031, 1548, 1895, 2495, 2519
Bse118I	RCCGGY	4 cut(s)	149, 176, 569, 2195
Bse1I	ACTGG	3 cut(s)	1900, 1925, 2402
Bse3DI	GCAATG	2 cut(s)	1908, 2382
Bse8I	GATNNNNATC	2 cut(s)	1242, 2410
BseBI	CCWGG	4 cut(s)	50, 260, 644, 2496
BseJI	GATNNNNATC	2 cut(s)	1242, 2410
BseLI	CCNNNNNNNGG	8 cut(s)	176, 265, 836, 1031, 1548, 1895, 2495, 2519
BseMI	GCAATG	2 cut(s)	1908, 2382
BseMII	CTCAG	9 cut(s)	384, 702, 1185, 1634, 2040, 2202, 2244, 2301, 2461
BseNI	ACTGG	3 cut(s)	1900, 1925, 2402
BsePI	GCGCGC	1 cut(s)	71
BseRI	GAGGAG	2 cut(s)	1375, 2416
BseSI	GKGCMC	2 cut(s)	258, 1978
BseXI	GCAGC	4 cut(s)	104, 657, 1309, 1993
BseYI	CCCAGC	3 cut(s)	720, 1262, 1510
BsgI	GTGCAG	2 cut(s)	23, 130
Bsh1236I	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BshFI	GGCC	9 cut(s)	180, 263, 1022, 1318, 1759, 1876, 1976, 2195, 2494
BshNI	GGYRCC	1 cut(s)	404
BsiHKAI	GWGCWC	2 cut(s)	1200, 2542
BsiHKCI	CYCGRG	1 cut(s)	1579
BsiWI	CGTACG	2 cut(s)	275, 886
BslFI	GGGAC	2 cut(s)	578, 1279
BslI	CCNNNNNNNGG	8 cut(s)	176, 265, 836, 1031, 1548, 1895, 2495, 2519
BsmAI	GTCTC	3 cut(s)	103, 1476, 2257
BsmBI	CGTCTC	1 cut(s)	103
BsmFI	GGGAC	2 cut(s)	578, 1279
BsmI	GAATGC	1 cut(s)	997
BsnI	GGCC	9 cut(s)	180, 263, 1022, 1318, 1759, 1876, 1976, 2195, 2494
BsoBI	CYCGRG	1 cut(s)	1579
Bsp120I	GGGCCC	1 cut(s)	1974
Bsp1286I	GDGCHC	4 cut(s)	258, 1200, 1978, 2542
Bsp1720I	GCTNAGC	4 cut(s)	351, 393, 1046, 2310
Bsp19I	CCATGG	1 cut(s)	1286
BspANI	GGCC	9 cut(s)	180, 263, 1022, 1318, 1759, 1876, 1976, 2195, 2494
BspCNI	CTCAG	9 cut(s)	385, 703, 1186, 1633, 2041, 2203, 2245, 2302, 2460
BspFNI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BspHI	TCATGA	1 cut(s)	967
BspMAI	CTGCAG	2 cut(s)	1983, 2436
BspMI	ACCTGC	2 cut(s)	87, 2452
BspPI	GGATC	7 cut(s)	569, 1221, 1532, 1591, 1656, 1880, 2173
BspT107I	GGYRCC	1 cut(s)	404
BsrBI	CCGCTC	2 cut(s)	159, 334
BsrDI	GCAATG	2 cut(s)	1908, 2382
BsrFI	RCCGGY	4 cut(s)	149, 176, 569, 2195
BsrI	ACTGG	3 cut(s)	1900, 1925, 2402
BssAI	RCCGGY	4 cut(s)	149, 176, 569, 2195
BssHII	GCGCGC	1 cut(s)	71
BssNI	GRCGYC	1 cut(s)	14
BssT1I	CCWWGG	5 cut(s)	1269, 1277, 1286, 2026, 2127
Bst2UI	CCWGG	4 cut(s)	50, 260, 644, 2496
Bst4CI	ACNGT	8 cut(s)	139, 268, 1225, 1304, 1356, 1374, 1442, 2246
Bst6I	CTCTTC	3 cut(s)	389, 1085, 2255
BstACI	GRCGYC	1 cut(s)	14
BstAPI	GCANNNNNTGC	1 cut(s)	1297
BstBAI	YACGTR	2 cut(s)	631, 655
BstC8I	GCNNGC	7 cut(s)	73, 130, 178, 336, 571, 1320, 1822
BstDSI	CCRYGG	4 cut(s)	181, 264, 345, 1286
BstEII	GGTNACC	1 cut(s)	2438
BstFNI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BstH2I	RGCGCY	3 cut(s)	109, 393, 2107
BstMAI	GTCTC	3 cut(s)	103, 1476, 2257
BstNI	CCWGG	4 cut(s)	50, 260, 644, 2496
BstNSI	RCATGY	1 cut(s)	1556
BstPI	GGTNACC	1 cut(s)	2438
BstSCI	CCNGG	7 cut(s)	48, 258, 546, 642, 980, 2489, 2494
BstSFI	CTRYAG	4 cut(s)	553, 1303, 1979, 2432
BstSLI	GKGCMC	2 cut(s)	258, 1978
BstUI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BstV1I	GCAGC	4 cut(s)	104, 657, 1309, 1993
BstX2I	RGATCY	4 cut(s)	1213, 1648, 1697, 2165
BstXI	CCANNNNNNTGG	1 cut(s)	1518
BstYI	RGATCY	4 cut(s)	1213, 1648, 1697, 2165
BsuI	GTATCC	2 cut(s)	1446, 2463
BsuRI	GGCC	9 cut(s)	180, 263, 1022, 1318, 1759, 1876, 1976, 2195, 2494
BtgI	CCRYGG	4 cut(s)	181, 264, 345, 1286
BtgZI	GCGATG	2 cut(s)	550, 2397
BtsI	GCAGTG	1 cut(s)	1857
BtsIMutI	CAGTG	6 cut(s)	1221, 1300, 1379, 1586, 1857, 2208
BveI	ACCTGC	2 cut(s)	87, 2452
Cac8I	GCNNGC	7 cut(s)	73, 130, 178, 336, 571, 1320, 1822
CaiI	CAGNNNCTG	1 cut(s)	1781
CciI	TCATGA	1 cut(s)	967
Cfr10I	RCCGGY	4 cut(s)	149, 176, 569, 2195
Cfr13I	GGNCC	9 cut(s)	52, 193, 473, 1021, 1266, 1316, 1974, 1975, 2329
Cfr42I	CCGCGG	1 cut(s)	348
CseI	GACGC	4 cut(s)	235, 271, 656, 856
Csp6I	GTAC	6 cut(s)	276, 640, 887, 2098, 2152, 2199
CviQI	GTAC	6 cut(s)	276, 640, 887, 2098, 2152, 2199
DraI	TTTAAA	1 cut(s)	1231
DraIII	CACNNNGTG	1 cut(s)	796
DrdI	GACNNNNNNGTC	1 cut(s)	239
DseDI	GACNNNNNNGTC	1 cut(s)	239
EaeI	YGGCCR	4 cut(s)	178, 1757, 2193, 2492
Eam1104I	CTCTTC	3 cut(s)	389, 1085, 2255
EarI	CTCTTC	3 cut(s)	389, 1085, 2255
EciI	GGCGGA	2 cut(s)	20, 101
Ecl136II	GAGCTC	1 cut(s)	2540
Eco130I	CCWWGG	5 cut(s)	1269, 1277, 1286, 2026, 2127
Eco24I	GRGCYC	2 cut(s)	1978, 2542
Eco47I	GGWCC	5 cut(s)	52, 193, 473, 1266, 2329
Eco47III	AGCGCT	1 cut(s)	2105
Eco53kI	GAGCTC	1 cut(s)	2540
Eco57I	CTGAAG	2 cut(s)	1521, 1899
Eco72I	CACGTG	2 cut(s)	631, 655
Eco88I	CYCGRG	1 cut(s)	1579
Eco91I	GGTNACC	1 cut(s)	2438
EcoICRI	GAGCTC	1 cut(s)	2540
EcoO109I	RGGNCCY	2 cut(s)	1974, 1975
EcoO65I	GGTNACC	1 cut(s)	2438
EcoRII	CCWGG	4 cut(s)	48, 258, 642, 2494
EcoT14I	CCWWGG	5 cut(s)	1269, 1277, 1286, 2026, 2127
EcoT22I	ATGCAT	2 cut(s)	1756, 2092
EcoT38I	GRGCYC	2 cut(s)	1978, 2542
ErhI	CCWWGG	5 cut(s)	1269, 1277, 1286, 2026, 2127
Esp3I	CGTCTC	1 cut(s)	103
FalI	AAGNNNNNCTT	4 cut(s)	590, 622, 923, 955
FaqI	GGGAC	2 cut(s)	578, 1279
FauI	CCCGC	5 cut(s)	164, 339, 352, 427, 943
FauNDI	CATATG	3 cut(s)	759, 1327, 1750
FbaI	TGATCA	1 cut(s)	2560
FblI	GTMKAC	1 cut(s)	552
FriOI	GRGCYC	2 cut(s)	1978, 2542
FspBI	CTAG	1 cut(s)	1652
GsaI	CCCAGC	3 cut(s)	724, 1266, 1514
GsuI	CTGGAG	1 cut(s)	2419
HaeII	RGCGCY	3 cut(s)	109, 393, 2107
HaeIII	GGCC	9 cut(s)	180, 263, 1022, 1318, 1759, 1876, 1976, 2195, 2494
HgaI	GACGC	4 cut(s)	235, 271, 656, 856
Hin1I	GRCGYC	1 cut(s)	14
HincII	GTYRAC	2 cut(s)	142, 846
HindII	GTYRAC	2 cut(s)	142, 846
HindIII	AAGCTT	2 cut(s)	353, 1043
HinfI	GANTC	5 cut(s)	233, 301, 317, 591, 1577
HphI	GGTGA	6 cut(s)	115, 217, 746, 1895, 2432, 2483
Hpy166II	GTNNAC	5 cut(s)	142, 528, 553, 846, 1111
Hpy8I	GTNNAC	5 cut(s)	142, 528, 553, 846, 1111
Hpy99I	CGWCG	5 cut(s)	16, 19, 22, 251, 428
HpyAV	CCTTC	9 cut(s)	65, 544, 580, 613, 687, 691, 875, 1874, 2453
HpyCH4III	ACNGT	8 cut(s)	139, 268, 1225, 1304, 1356, 1374, 1442, 2246
HpyCH4IV	ACGT	7 cut(s)	14, 240, 630, 654, 1253, 2154, 2481
HpySE526I	ACGT	7 cut(s)	14, 240, 630, 654, 1253, 2154, 2481
Hsp92I	GRCGYC	1 cut(s)	14
KroI	GCCGGC	2 cut(s)	176, 569
KroNI	GCCGGC	2 cut(s)	178, 571
Ksp22I	TGATCA	1 cut(s)	2560
KspI	CCGCGG	1 cut(s)	348
LmnI	GCTCC	9 cut(s)	22, 158, 164, 185, 210, 811, 1279, 1778, 2537
Lsp1109I	GCAGC	4 cut(s)	104, 657, 1309, 1993
MaeI	CTAG	1 cut(s)	1652
MaeII	ACGT	7 cut(s)	14, 240, 630, 654, 1253, 2154, 2481
MaeIII	GTNAC	5 cut(s)	626, 1006, 1486, 2438, 2477
MbiI	CCGCTC	2 cut(s)	159, 334
MfeI	CAATTG	2 cut(s)	1491, 2564
MflI	RGATCY	4 cut(s)	1213, 1648, 1697, 2165
MhlI	GDGCHC	4 cut(s)	258, 1200, 1978, 2542
MlsI	TGGCCA	1 cut(s)	1759
MluNI	TGGCCA	1 cut(s)	1759
MlyI	GAGTC	4 cut(s)	227, 326, 600, 1571
MmeI	TCCRAC	3 cut(s)	186, 1051, 2307
Mox20I	TGGCCA	1 cut(s)	1759
Mph1103I	ATGCAT	2 cut(s)	1756, 2092
MreI	CGCCGGCG	1 cut(s)	569
MroNI	GCCGGC	2 cut(s)	176, 569
MroXI	GAANNNNTTC	2 cut(s)	989, 1239
MscI	TGGCCA	1 cut(s)	1759
MseI	TTAA	3 cut(s)	1230, 1427, 1431
MslI	CAYNNNNRTG	4 cut(s)	1413, 1419, 1753, 2271
Msp20I	TGGCCA	1 cut(s)	1759
MspA1I	CMGCKG	2 cut(s)	117, 347
MspR9I	CCNGG	7 cut(s)	50, 260, 548, 644, 982, 2491, 2496
MunI	CAATTG	2 cut(s)	1491, 2564
Mva1269I	GAATGC	1 cut(s)	997
MvaI	CCWGG	4 cut(s)	50, 260, 644, 2496
MvnI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
NaeI	GCCGGC	2 cut(s)	178, 571
NciI	CCSGG	3 cut(s)	548, 982, 2491
NcoI	CCATGG	1 cut(s)	1286
NdeI	CATATG	3 cut(s)	759, 1327, 1750
NgoMIV	GCCGGC	2 cut(s)	176, 569
NmeAIII	GCCGAG	2 cut(s)	214, 840
NmuCI	GTSAC	3 cut(s)	626, 1006, 2438
NsiI	ATGCAT	2 cut(s)	1756, 2092
NspI	RCATGY	1 cut(s)	1556
PacI	TTAATTAA	1 cut(s)	1431
PagI	TCATGA	1 cut(s)	967
PauI	GCGCGC	1 cut(s)	71
PcsI	WCGNNNNNNNCGW	1 cut(s)	246
PctI	GAATGC	1 cut(s)	997
PdiI	GCCGGC	2 cut(s)	178, 571
PdmI	GAANNNNTTC	2 cut(s)	989, 1239
PfeI	GAWTC	1 cut(s)	301
Pfl23II	CGTACG	2 cut(s)	275, 886
PflFI	GACNNNGTC	3 cut(s)	16, 95, 137
PleI	GAGTC	4 cut(s)	227, 325, 599, 1571
PmaCI	CACGTG	2 cut(s)	631, 655
PmlI	CACGTG	2 cut(s)	631, 655
PpsI	GAGTC	4 cut(s)	227, 325, 599, 1571
Ppu21I	YACGTR	2 cut(s)	631, 655
PsiI	TTATAA	3 cut(s)	705, 1340, 1767
Psp124BI	GAGCTC	1 cut(s)	2542
Psp6I	CCWGG	4 cut(s)	48, 258, 642, 2494
PspCI	CACGTG	2 cut(s)	631, 655
PspEI	GGTNACC	1 cut(s)	2438
PspFI	CCCAGC	3 cut(s)	720, 1262, 1510
PspGI	CCWGG	4 cut(s)	48, 258, 642, 2494
PspLI	CGTACG	2 cut(s)	275, 886
PspOMI	GGGCCC	1 cut(s)	1974
PspPI	GGNCC	9 cut(s)	52, 193, 473, 1021, 1266, 1316, 1974, 1975, 2329
PstI	CTGCAG	2 cut(s)	1983, 2436
PstNI	CAGNNNCTG	1 cut(s)	1781
PsuI	RGATCY	4 cut(s)	1213, 1648, 1697, 2165
PsyI	GACNNNGTC	3 cut(s)	16, 95, 137
PteI	GCGCGC	1 cut(s)	71
RsaI	GTAC	6 cut(s)	277, 641, 888, 2099, 2153, 2200
RsaNI	GTAC	6 cut(s)	276, 640, 887, 2098, 2152, 2199
RseI	CAYNNNNRTG	4 cut(s)	1413, 1419, 1753, 2271
SacI	GAGCTC	1 cut(s)	2542
SacII	CCGCGG	1 cut(s)	348
SaqAI	TTAA	3 cut(s)	1230, 1427, 1431
Sau96I	GGNCC	9 cut(s)	52, 193, 473, 1021, 1266, 1316, 1974, 1975, 2329
SchI	GAGTC	4 cut(s)	227, 326, 600, 1571
ScrFI	CCNGG	7 cut(s)	50, 260, 548, 644, 982, 2491, 2496
SduI	GDGCHC	4 cut(s)	258, 1200, 1978, 2542
SfcI	CTRYAG	4 cut(s)	553, 1303, 1979, 2432
Sfr303I	CCGCGG	1 cut(s)	348
SgrAI	CRCCGGYG	1 cut(s)	569
SgrBI	CCGCGG	1 cut(s)	348
SinI	GGWCC	5 cut(s)	52, 193, 473, 1266, 2329
SmiMI	CAYNNNNRTG	4 cut(s)	1413, 1419, 1753, 2271
SmlI	CTYRAG	3 cut(s)	356, 863, 2001
SmoI	CTYRAG	3 cut(s)	356, 863, 2001
SspMI	CTAG	1 cut(s)	1652
SstI	GAGCTC	1 cut(s)	2542
StyD4I	CCNGG	7 cut(s)	48, 258, 546, 642, 980, 2489, 2494
StyI	CCWWGG	5 cut(s)	1269, 1277, 1286, 2026, 2127
TaaI	ACNGT	8 cut(s)	139, 268, 1225, 1304, 1356, 1374, 1442, 2246
TaiI	ACGT	7 cut(s)	17, 243, 633, 657, 1256, 2157, 2484
TaqI	TCGA	5 cut(s)	66, 426, 537, 1218, 2293
TauI	GCSGC	6 cut(s)	117, 350, 437, 676, 837, 840
TfiI	GAWTC	1 cut(s)	301
Tru1I	TTAA	3 cut(s)	1230, 1427, 1431
Tru9I	TTAA	3 cut(s)	1230, 1427, 1431
TscAI	CASTG	6 cut(s)	1228, 1307, 1379, 1593, 1857, 2215
TseFI	GTSAC	3 cut(s)	626, 1006, 2438
TseI	GCWGC	4 cut(s)	117, 670, 1322, 1981
Tsp45I	GTSAC	3 cut(s)	626, 1006, 2438
TspDTI	ATGAA	9 cut(s)	459, 987, 1070, 1232, 1437, 1702, 1734, 2111, 2517
TspGWI	ACGGA	4 cut(s)	198, 598, 705, 2445
TspRI	CASTG	6 cut(s)	1228, 1307, 1379, 1593, 1857, 2215
Tth111I	GACNNNGTC	3 cut(s)	16, 95, 137
VpaK11BI	GGWCC	5 cut(s)	52, 193, 473, 1266, 2329
XapI	RAATTY	3 cut(s)	447, 1911, 2506
XceI	RCATGY	1 cut(s)	1556
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1284
XmiI	GTMKAC	1 cut(s)	552
XmnI	GAANNNNTTC	2 cut(s)	989, 1239
XspI	CTAG	1 cut(s)	1652
ZraI	GACGTC	1 cut(s)	15
Zsp2I	ATGCAT	2 cut(s)	1756, 2092

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.