Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000855

Physical Location & Seq

Genomic Coordinates Reverse (-)

19503639 .. 19514883

Gene Structure 11245 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1237100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2568 bp

ATGGCCGAAAAGACGTCGGAGCGGGTGACCTACCTGCACGGCGACCTCGACCTCACAATCATCGAAGCCCGCCACCTCCCCAACATGGACATAGTCTCCGAGCGCTTCCGCCGCTGCTTCACCGCCTGCGACACCATCAACATATCCTGCAACCGCTCCGCCGAAGACGACGGCCGAGACCGCAAGCCCCACGGACCCCACCGCAAGATCATAACCAGCGACCCCTACGTCACCGTTGTCGTCCCCCAGACCACCGTCGCCCGGACACGTGTCATGAAGAACTCCCAGAACCCGCAGTGGAAGGAGCACTTCTTTATCCCGCTGGCTCACCCCGTGACTAACCTCGAGTTTCAGGTCAAAGATAACGACGTCTTCGGCGCCGAGATCATCGGAACCGCCAAGATCCCAGCCGAGAAAATCGCCACCGGAGAGCCCATCTCCGGGTGGTTCCCGATTATCGGACCTTCCGGGAAGCCCCCGAAACCCGCCTCGGCGATCCGGGTCGAATTGCAGTTCACGCCGTTCGAGAAAAACCCATTGTACAAGCGAGGCATAGCGGGGGATCCGGAACACAAGGGCGTGCCGCACACGTATTTCCCGCTCCGGAAGGGAAGCTCCGTGCGATTGTACCAAGACGCGCACTGCCCGGAGGGGCTGCTGCCGGAGATTCAGTTGGACGGCGGGAAGGTGTATAAGCAGGAGAATACGTGGGAGGACATTTGCTATGCGATTTCGGAGGCGCACCACTTGATTTACATTGTGGGTTGGTCGGTGTTTCACAAGGTGCGGCTGATTCGGGAGCCGAGCCGGCCGCTGCCGCGCGGCGGAAATTTGACGCTCGGCGATTTGCTGAAGTACAAGTCGGAGGAGGGCGTGAGGGTGTTGCTGTTGGTGTGGGACGATAAGACCTCGCACGACAAGTTCTGCTTCAAAACGGCAGGAATGATGGCGACGCATGATGAAGAAACAAGGAGGTTTTTCAAGCATTCATCTGTAAATTGTGTTCTGGCAACTCGCTACGCTAGCAGTAAGCTTAGCATCATAAAACAACAGGTTGTTGGAACCCTCTTCACCCACCATCAAAAATGTGTTCTTGTGGACACACAGGCATATGGTAATAATCGGAAGATAACTGCGTTTTTAGGAGGTCTTGATCTTTGTGATGGTCGTTATGACACACCCGAGCACAGACTGTTTCGTGATCTTAACACTGTATTTGAGGGTGATTTTCATCAACCTACTTTTCCTGCTGGAACCAAGGCTCCAAGAGAACCATGGCATGACTTGCACTGTAGAATAGATGGGCCTGCTGCATATGATGTTCTTATAAACTTTGAGCAACGTTGGAGGAAAGCAACACAGTGGAAGGAGTTTGGACTACTACGTAAGAAGGTTTCACATTGGCATGATGATGCTTTGATAAAAATTGACCGCATCTCCTGGATACTAAGTCCTCCCCTATCTGTTTCAAAGGATAGTACAACTATTCCAGAAGACGACCCTTCCTTGTGGGTTAACCGTGAAGAAGATCCTGAAGATTGGCATGTTCAGATTTTCCGATCCATTGACTCAGGATCACTGAAGGGATTTCCCAAAGCTGGCCGTGCAGCTGAGGCCCAGAATCTTATTTGCTCAAAGAACCTGGTAATAGATAAAAGCATTCAAACAGCATACATCCATGCAATCAGATCGGCCCAACATTTTATATATATTGAAAATCAGTATTTTCTTGGTTCATCATATGCGTGGCCAGATTATGAAAATGCAGGAGCTGATAATCTTATCCCAATGGAGTTGGCATTAAAGATTGTTAGCAAAATTAAAGCTAATGAGAGATTTACAGTCTATGTCGTTTTACCTATGTGGCCTGAAGGTGATCCAAAAACTGCTGCAATGCAAGAAATTCTCTTCTGGCAGAGCCAGACAATGCAAGCGATGTATGGTGTCGTTGCACAGGCACTGAAATCGGCGCAGCTTCAGGACTCACATCCTCAAGATTACCTAAGTTTCTATTGCCTTGGTAAACGGGAAATACCTCCTGAGGATATGGCAAATGACAATGCATCTTCGGTTTCCGATTCATTTAAATATAAGCGGTTCATGATTTATGTTCATGCCAAGGGTATGGTAGTAGATGACGAGTATGTAATGCTGGGATCTGCCAATATCAACCAAAGGTCAATGGCTGGTACAAAAGACACTGAGATAGCTATGGGTGCGTATCAACCCCATCACACTTGGGCTGCGAGGAAGAAACATCCGTTTGGTCAGATATTTGGATATCGAATGTCACTTTGGGCCGAGCACCTTGGTATGTTGGATCCATGCTTCCAAGAGCCTGAGAGTTTGGAATGTATGAGGAAAGTAAATGCGATAGCAGACGAAAACTGGAAGAGATTCACAACACCAGACTTTACACTACTGCAGGGTCACCTTCTGAAGTATCCTATACAGGTAGATGTTGATGGGAAGGTCGGCCCCCTTCCGGGACATGAAAACTTCCCTGATCTTGGTGGTAAAGTACTTGGAGCTCATGCCGCCACAGTTCCTGATATGCTCACAACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006950 GO:0008081 GO:0008150 GO:0008152 GO:0008219 GO:0009266 GO:0009409 GO:0009506 GO:0009628 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010119 GO:0010646 GO:0010647 GO:0012501 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019637 GO:0023051 GO:0023056 GO:0030054 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046473 GO:0046486 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0090333 GO:1901419 GO:1901421 GO:1905957 GO:1905959

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

856

Amino Acids

97.05

Weight (kDa)

6.75

Isoelectric Point (pI)

35.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	15 - 151	8.1e-18	C2 domain
PLDc_2	PF13091	698 - 741	6.3e-06	PLD-like domain
PLDc	PF00614	702 - 727	5.7e-07	Phospholipase D Active site motif
PLD_C	PF12357	775 - 844	8.4e-30	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000855)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35790 AT4G35790 AT4G35790
fragaria_vesca	FvH4_1g11000 FvH4_1g11020 FvH4_1g11020 FvH4_2g37900 FvH4_5g01272 FvH4_5g28780 FvH4_5g28800 FvH4_7g08510 FvH4_7g08510
malus_domestica	MD02G1123100.v1.1 MD08G1149700.v1.1 MD08G1237200.v1.1 MD15G1006400.v1.1 MD15G1237100.v1.1
prunus_persica	Prupe.1G359000_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.7G175400_v2.0.a1
pyrus_communis	pycom02g09600 pycom15g00480 pycom15g21090
rosa_chinensis	RchiOBHm_Chr2g0098271 RchiOBHm_Chr6g0303711 RchiOBHm_Chr7g0221001 RchiOBHm_Chr7g0221011
rosa_laevigata	RLG00000002185 RLG00000011053 RLG00000016781
rosa_multiflora	Rmu_co8414259.1_g000001 Rmu_co8436567.1_g000002 Rmu_co8452585.1_g000001 Rmu_sc0001024.1_g000003 Rmu_sc0002105.1_g000010 Rmu_sc0002105.1_g000018 Rmu_sc0002105.1_g000020 Rmu_sc0009922.1_g000005 Rmu_sc0039265.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144090 Rroxscaffold_3G00238820 Rroxscaffold_7G00164460
rosa_rugosa	Rorug02G0071800 Rorug06G0324200 Rorug07G0199200 Rorug07G0199300 Rorug07G0199400 Rorug07G0199500 Rorug07G0199600
rosa_samantha	Rh1CG003000 Rh2AG118900 Rh2BG121800 Rh2CG123400 Rh2DG123500 Rh6AG438500 Rh6BG464500 Rh6CG450800 Rh6DG437500 Rh7AG339200 Rh7BG329900 Rh7CG357200 Rh7DG337900
rosa_wichuraiana	Rw2G009320 Rw6G037950 Rw7G028850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1328
AasI	GACNNNNNNGTC	1 cut(s)	227
AatII	GACGTC	2 cut(s)	17, 372
Acc36I	ACCTGC	1 cut(s)	42
AccB1I	GGYRCC	1 cut(s)	377
AccBSI	CCGCTC	3 cut(s)	22, 156, 601
AccII	CGCG	3 cut(s)	638, 820, 822
AccIII	TCCGGA	2 cut(s)	565, 603
AclI	AACGTT	1 cut(s)	1342
AcoI	YGGCCR	5 cut(s)	3, 172, 809, 1600, 1748
AcsI	RAATTY	2 cut(s)	829, 1902
AcuI	CTGAAG	6 cut(s)	872, 1554, 1601, 1890, 1961, 2459
AcvI	CACGTG	1 cut(s)	269
AcyI	GRCGYC	3 cut(s)	14, 369, 378
AdeI	CACNNNGTG	2 cut(s)	334, 784
AfaI	GTAC	6 cut(s)	542, 629, 857, 1480, 2191, 2523
AfeI	AGCGCT	1 cut(s)	104
AflIII	ACRYGT	3 cut(s)	266, 268, 588
AgsI	TTSAA	5 cut(s)	931, 982, 1470, 1664, 1715
AjnI	CCWGG	2 cut(s)	1439, 1641
AjuI	GAANNNNNNNTTGG	2 cut(s)	2278, 2310
AloI	GAACNNNNNNTCC	2 cut(s)	1246, 1278
AluBI	AGCT	9 cut(s)	615, 1033, 1598, 1610, 1772, 1826, 1975, 2210, 2531
AluI	AGCT	9 cut(s)	615, 1033, 1598, 1610, 1772, 1826, 1975, 2210, 2531
Alw21I	GWGCWC	4 cut(s)	309, 1188, 2307, 2533
Alw26I	GTCTC	2 cut(s)	100, 171
AlwNI	CAGNNNCTG	3 cut(s)	1772, 1960, 2549
Ama87I	CYCGRG	2 cut(s)	344, 1181
Aor13HI	TCCGGA	2 cut(s)	565, 603
Aor51HI	AGCGCT	1 cut(s)	104
ApeKI	GCWGC	9 cut(s)	114, 655, 658, 814, 1310, 1607, 1889, 1972, 2243
ApoI	RAATTY	2 cut(s)	829, 1902
Asp700I	GAANNNNTTC	1 cut(s)	2396
AspLEI	GCGC	6 cut(s)	105, 380, 640, 742, 822, 1972
AspS9I	GGNCC	7 cut(s)	194, 461, 1304, 1615, 1693, 2298, 2477
AsuC2I	CCSGG	6 cut(s)	262, 442, 469, 500, 647, 2487
AsuHPI	GGTGA	8 cut(s)	37, 112, 223, 320, 1063, 1235, 1886, 2423
AsuNHI	GCTAGC	1 cut(s)	1022
AvaI	CYCGRG	2 cut(s)	344, 1181
AvaII	GGWCC	2 cut(s)	194, 461
AxyI	CCTNAGG	1 cut(s)	2040
BalI	TGGCCA	1 cut(s)	1750
BamHI	GGATCC	2 cut(s)	562, 2320
BanI	GGYRCC	1 cut(s)	377
BanII	GRGCYC	2 cut(s)	435, 2533
BbrPI	CACGTG	1 cut(s)	269
BbsI	GAAGAC	3 cut(s)	171, 364, 1500
Bbv12I	GWGCWC	4 cut(s)	309, 1188, 2307, 2533
BbvCI	CCTCAGC	1 cut(s)	1611
BbvI	GCAGC	9 cut(s)	101, 642, 645, 801, 1297, 1619, 1876, 1984, 2230
BccI	CCATC	8 cut(s)	143, 443, 940, 1086, 1157, 1295, 2238, 2459
BceAI	ACGGC	6 cut(s)	55, 187, 505, 694, 951, 1587
BcgI	CGANNNNNNTGC	4 cut(s)	1671, 1705, 1947, 1981
BciT130I	CCWGG	2 cut(s)	1441, 1643
BciVI	GTATCC	2 cut(s)	1437, 2454
BcnI	CCSGG	6 cut(s)	262, 442, 469, 500, 647, 2487
BcoDI	GTCTC	2 cut(s)	100, 171
BfaI	CTAG	1 cut(s)	1023
BfmI	CTRYAG	2 cut(s)	1291, 2423
BfoI	RGCGCY	2 cut(s)	106, 381
BfuAI	ACCTGC	1 cut(s)	42
BfuI	GTATCC	2 cut(s)	1437, 2454
BglI	GCCNNNNNGGC	1 cut(s)	808
BlpI	GCTNAGC	1 cut(s)	1034
BmcAI	AGTACT	1 cut(s)	2523
Bme1390I	CCNGG	8 cut(s)	262, 442, 469, 500, 647, 1441, 1643, 2487
Bme18I	GGWCC	2 cut(s)	194, 461
BmeT110I	CYCGRG	2 cut(s)	344, 1181
BmgT120I	GGNCC	7 cut(s)	194, 461, 1304, 1615, 1693, 2298, 2477
BmrFI	CCNGG	8 cut(s)	262, 442, 469, 500, 647, 1441, 1643, 2487
BmsI	GCATC	4 cut(s)	1047, 1402, 1443, 2072
BmtI	GCTAGC	1 cut(s)	1026
BoxI	GACNNNNGTC	2 cut(s)	254, 269
BpiI	GAAGAC	3 cut(s)	171, 364, 1500
BplI	GAGNNNNNCTC	2 cut(s)	422, 454
Bpu10I	CCTNAGC	1 cut(s)	1611
Bpu1102I	GCTNAGC	1 cut(s)	1034
BpuEI	CTTGAG	1 cut(s)	1977
BpuMI	CCSGG	6 cut(s)	262, 442, 469, 500, 647, 2487
BsaAI	YACGTR	4 cut(s)	269, 591, 708, 1385
BsaBI	GATNNNNATC	1 cut(s)	1230
BsaHI	GRCGYC	3 cut(s)	14, 369, 378
BsaI	GGTCTC	1 cut(s)	171
BsaJI	CCNNGG	7 cut(s)	190, 489, 1257, 1274, 2017, 2118, 2308
BsaWI	WCCGGW	3 cut(s)	425, 565, 603
Bse118I	RCCGGY	1 cut(s)	807
Bse1I	ACTGG	1 cut(s)	2393
Bse21I	CCTNAGG	1 cut(s)	2040
Bse3DI	GCAATG	1 cut(s)	1899
Bse8I	GATNNNNATC	1 cut(s)	1230
BseAI	TCCGGA	2 cut(s)	565, 603
BseBI	CCWGG	2 cut(s)	1441, 1643
BseDI	CCNNGG	7 cut(s)	190, 489, 1257, 1274, 2017, 2118, 2308
BseGI	GGATG	3 cut(s)	1674, 1987, 2257
BseJI	GATNNNNATC	1 cut(s)	1230
BseMI	GCAATG	1 cut(s)	1899
BseMII	CTCAG	5 cut(s)	1584, 1602, 2031, 2193, 2331
BseNI	ACTGG	1 cut(s)	2393
BseRI	GAGGAG	1 cut(s)	881
BseX3I	CGGCCG	2 cut(s)	172, 809
BseXI	GCAGC	9 cut(s)	101, 642, 645, 801, 1297, 1619, 1876, 1984, 2230
BseYI	CCCAGC	2 cut(s)	406, 2152
BsgI	GTGCAG	2 cut(s)	20, 1626
Bsh1236I	CGCG	3 cut(s)	638, 820, 822
Bsh1285I	CGRYCG	2 cut(s)	175, 812
BshNI	GGYRCC	1 cut(s)	377
BsiEI	CGRYCG	2 cut(s)	175, 812
BsiHKAI	GWGCWC	4 cut(s)	309, 1188, 2307, 2533
BsiHKCI	CYCGRG	2 cut(s)	344, 1181
BslFI	GGGAC	3 cut(s)	227, 911, 2502
BsmAI	GTCTC	2 cut(s)	100, 171
BsmFI	GGGAC	3 cut(s)	227, 911, 2502
BsmI	GAATGC	2 cut(s)	985, 1659
Bso31I	GGTCTC	1 cut(s)	171
BsoBI	CYCGRG	2 cut(s)	344, 1181
Bsp1286I	GDGCHC	5 cut(s)	309, 435, 1188, 2307, 2533
Bsp13I	TCCGGA	2 cut(s)	565, 603
Bsp1407I	TGTACA	1 cut(s)	540
Bsp1720I	GCTNAGC	1 cut(s)	1034
Bsp19I	CCATGG	1 cut(s)	1274
BspCNI	CTCAG	5 cut(s)	1583, 1603, 2032, 2194, 2332
BspEI	TCCGGA	2 cut(s)	565, 603
BspFNI	CGCG	3 cut(s)	638, 820, 822
BspHI	TCATGA	2 cut(s)	273, 2100
BspMAI	CTGCAG	1 cut(s)	2427
BspMI	ACCTGC	1 cut(s)	42
BspOI	GCTAGC	1 cut(s)	1026
BspT107I	GGYRCC	1 cut(s)	377
BspTNI	GGTCTC	1 cut(s)	171
BsrBI	CCGCTC	3 cut(s)	22, 156, 601
BsrDI	GCAATG	1 cut(s)	1899
BsrFI	RCCGGY	1 cut(s)	807
BsrGI	TGTACA	1 cut(s)	540
BsrI	ACTGG	1 cut(s)	2393
BssAI	RCCGGY	1 cut(s)	807
BssECI	CCNNGG	7 cut(s)	190, 489, 1257, 1274, 2017, 2118, 2308
BssNI	GRCGYC	3 cut(s)	14, 369, 378
BssT1I	CCWWGG	5 cut(s)	1257, 1274, 2017, 2118, 2308
Bst2UI	CCWGG	2 cut(s)	1441, 1643
Bst4CI	ACNGT	9 cut(s)	235, 256, 1194, 1213, 1292, 1362, 1520, 1843, 2545
Bst6I	CTCTTC	3 cut(s)	1073, 1913, 2387
BstACI	GRCGYC	3 cut(s)	14, 369, 378
BstAPI	GCANNNNNTGC	1 cut(s)	1285
BstAUI	TGTACA	1 cut(s)	540
BstBAI	YACGTR	4 cut(s)	269, 591, 708, 1385
BstDEI	CTNAG	8 cut(s)	1034, 1448, 1570, 1611, 2003, 2040, 2202, 2340
BstDSI	CCRYGG	2 cut(s)	190, 1274
BstEII	GGTNACC	2 cut(s)	25, 2429
BstF5I	GGATG	3 cut(s)	1674, 1987, 2257
BstFNI	CGCG	3 cut(s)	638, 820, 822
BstH2I	RGCGCY	2 cut(s)	106, 381
BstHHI	GCGC	6 cut(s)	105, 380, 640, 742, 822, 1972
BstMAI	GTCTC	2 cut(s)	100, 171
BstMCI	CGRYCG	2 cut(s)	175, 812
BstNI	CCWGG	2 cut(s)	1441, 1643
BstNSI	RCATGY	1 cut(s)	1547
BstPAI	GACNNNNGTC	2 cut(s)	254, 269
BstPI	GGTNACC	2 cut(s)	25, 2429
BstSCI	CCNGG	8 cut(s)	260, 440, 467, 498, 645, 1439, 1641, 2485
BstSFI	CTRYAG	2 cut(s)	1291, 2423
BstSNI	TACGTA	1 cut(s)	1385
BstUI	CGCG	3 cut(s)	638, 820, 822
BstV1I	GCAGC	9 cut(s)	101, 642, 645, 801, 1297, 1619, 1876, 1984, 2230
BstV2I	GAAGAC	3 cut(s)	171, 364, 1500
BstX2I	RGATCY	5 cut(s)	402, 562, 1528, 2156, 2320
BstXI	CCANNNNNNTGG	1 cut(s)	2125
BstYI	RGATCY	5 cut(s)	402, 562, 1528, 2156, 2320
BstZI	CGGCCG	2 cut(s)	172, 809
Bsu36I	CCTNAGG	1 cut(s)	2040
BsuI	GTATCC	2 cut(s)	1437, 2454
BtgI	CCRYGG	2 cut(s)	190, 1274
BtgZI	GCGATG	1 cut(s)	1949
BtsCI	GGATG	3 cut(s)	1674, 1987, 2257
BtsI	GCAGTG	2 cut(s)	302, 640
BtsIMutI	CAGTG	8 cut(s)	302, 640, 1209, 1288, 1367, 1577, 1958, 2199
BveI	ACCTGC	1 cut(s)	42
CaiI	CAGNNNCTG	3 cut(s)	1772, 1960, 2549
CciI	TCATGA	2 cut(s)	273, 2100
CfoI	GCGC	6 cut(s)	105, 380, 640, 742, 822, 1972
Cfr10I	RCCGGY	1 cut(s)	807
Cfr13I	GGNCC	7 cut(s)	194, 461, 1304, 1615, 1693, 2298, 2477
CseI	GACGC	3 cut(s)	644, 844, 961
CsiI	ACCWGGT	1 cut(s)	1641
Csp6I	GTAC	6 cut(s)	541, 628, 856, 1479, 2190, 2522
CviQI	GTAC	6 cut(s)	541, 628, 856, 1479, 2190, 2522
DdeI	CTNAG	8 cut(s)	1034, 1448, 1570, 1611, 2003, 2040, 2202, 2340
DinI	GGCGCC	1 cut(s)	379
DraI	TTTAAA	1 cut(s)	2086
DraIII	CACNNNGTG	2 cut(s)	334, 784
DrdI	GACNNNNNNGTC	1 cut(s)	227
DseDI	GACNNNNNNGTC	1 cut(s)	227
EaeI	YGGCCR	5 cut(s)	3, 172, 809, 1600, 1748
EagI	CGGCCG	2 cut(s)	172, 809
Eam1104I	CTCTTC	3 cut(s)	1073, 1913, 2387
EarI	CTCTTC	3 cut(s)	1073, 1913, 2387
EciI	GGCGGA	3 cut(s)	98, 148, 840
Ecl136II	GAGCTC	1 cut(s)	2531
EclXI	CGGCCG	2 cut(s)	172, 809
Eco105I	TACGTA	1 cut(s)	1385
Eco130I	CCWWGG	5 cut(s)	1257, 1274, 2017, 2118, 2308
Eco24I	GRGCYC	2 cut(s)	435, 2533
Eco31I	GGTCTC	1 cut(s)	171
Eco32I	GATATC	1 cut(s)	2282
Eco47I	GGWCC	2 cut(s)	194, 461
Eco47III	AGCGCT	1 cut(s)	104
Eco52I	CGGCCG	2 cut(s)	172, 809
Eco53kI	GAGCTC	1 cut(s)	2531
Eco57I	CTGAAG	6 cut(s)	872, 1554, 1601, 1890, 1961, 2459
Eco72I	CACGTG	1 cut(s)	269
Eco81I	CCTNAGG	1 cut(s)	2040
Eco88I	CYCGRG	2 cut(s)	344, 1181
Eco91I	GGTNACC	2 cut(s)	25, 2429
EcoICRI	GAGCTC	1 cut(s)	2531
EcoO65I	GGTNACC	2 cut(s)	25, 2429
EcoRII	CCWGG	2 cut(s)	1439, 1641
EcoRV	GATATC	1 cut(s)	2282
EcoT14I	CCWWGG	5 cut(s)	1257, 1274, 2017, 2118, 2308
EcoT22I	ATGCAT	1 cut(s)	2065
EcoT38I	GRGCYC	2 cut(s)	435, 2533
EgeI	GGCGCC	1 cut(s)	379
EheI	GGCGCC	1 cut(s)	379
ErhI	CCWWGG	5 cut(s)	1257, 1274, 2017, 2118, 2308
FalI	AAGNNNNNCTT	4 cut(s)	293, 325, 911, 943
FaqI	GGGAC	3 cut(s)	227, 911, 2502
FauI	CCCGC	8 cut(s)	15, 77, 300, 327, 493, 550, 606, 674
FauNDI	CATATG	3 cut(s)	1111, 1315, 1741
FokI	GGATG	3 cut(s)	1661, 1974, 2244
FriOI	GRGCYC	2 cut(s)	435, 2533
FspBI	CTAG	1 cut(s)	1023
GlaI	GCGC	6 cut(s)	104, 379, 639, 741, 821, 1971
GsaI	CCCAGC	2 cut(s)	410, 2156
HaeII	RGCGCY	2 cut(s)	106, 381
HgaI	GACGC	3 cut(s)	644, 844, 961
HhaI	GCGC	6 cut(s)	105, 380, 640, 742, 822, 1972
Hin1I	GRCGYC	3 cut(s)	14, 369, 378
Hin6I	GCGC	6 cut(s)	103, 378, 638, 740, 820, 1970
HinP1I	GCGC	6 cut(s)	103, 378, 638, 740, 820, 1970
HincII	GTYRAC	1 cut(s)	1516
HindII	GTYRAC	1 cut(s)	1516
HindIII	AAGCTT	1 cut(s)	1031
HinfI	GANTC	7 cut(s)	667, 793, 1568, 1621, 1982, 2078, 2397
HpaI	GTTAAC	1 cut(s)	1516
HphI	GGTGA	8 cut(s)	37, 112, 223, 320, 1063, 1235, 1886, 2423
Hpy166II	GTNNAC	4 cut(s)	516, 1099, 1516, 2024
Hpy8I	GTNNAC	4 cut(s)	516, 1099, 1516, 2024
Hpy99I	CGWCG	5 cut(s)	19, 173, 260, 371, 955
HpyCH4III	ACNGT	9 cut(s)	235, 256, 1194, 1213, 1292, 1362, 1520, 1843, 2545
HpyCH4IV	ACGT	8 cut(s)	14, 228, 268, 369, 590, 707, 1342, 1384
HpyF3I	CTNAG	8 cut(s)	1034, 1448, 1570, 1611, 2003, 2040, 2202, 2340
HpySE526I	ACGT	8 cut(s)	14, 228, 268, 369, 590, 707, 1342, 1384
Hsp92I	GRCGYC	3 cut(s)	14, 369, 378
HspAI	GCGC	6 cut(s)	103, 378, 638, 740, 820, 1970
KasI	GGCGCC	1 cut(s)	377
Kpn2I	TCCGGA	2 cut(s)	565, 603
KroI	GCCGGC	1 cut(s)	807
KroNI	GCCGGC	1 cut(s)	809
KspAI	GTTAAC	1 cut(s)	1516
LmnI	GCTCC	9 cut(s)	19, 161, 304, 606, 620, 799, 1267, 1769, 2528
Lsp1109I	GCAGC	9 cut(s)	101, 642, 645, 801, 1297, 1619, 1876, 1984, 2230
LweI	GCATC	4 cut(s)	1047, 1402, 1443, 2072
MabI	ACCWGGT	1 cut(s)	1641
MaeI	CTAG	1 cut(s)	1023
MaeII	ACGT	8 cut(s)	14, 228, 268, 369, 590, 707, 1342, 1384
MaeIII	GTNAC	5 cut(s)	25, 229, 334, 2289, 2429
MbiI	CCGCTC	3 cut(s)	22, 156, 601
MflI	RGATCY	5 cut(s)	402, 562, 1528, 2156, 2320
MhlI	GDGCHC	5 cut(s)	309, 435, 1188, 2307, 2533
MlsI	TGGCCA	1 cut(s)	1750
MluCI	AATT	6 cut(s)	506, 829, 997, 1425, 1818, 1902
MluNI	TGGCCA	1 cut(s)	1750
Mly113I	GGCGCC	1 cut(s)	378
MlyI	GAGTC	2 cut(s)	1562, 1976
MmeI	TCCRAC	5 cut(s)	654, 843, 1039, 1325, 2298
Mox20I	TGGCCA	1 cut(s)	1750
Mph1103I	ATGCAT	1 cut(s)	2065
MroI	TCCGGA	2 cut(s)	565, 603
MroNI	GCCGGC	1 cut(s)	807
MroXI	GAANNNNTTC	1 cut(s)	2396
MscI	TGGCCA	1 cut(s)	1750
MseI	TTAA	5 cut(s)	1206, 1515, 1802, 1821, 2085
MslI	CAYNNNNRTG	3 cut(s)	1404, 1410, 1744
Msp20I	TGGCCA	1 cut(s)	1750
MspA1I	CMGCKG	4 cut(s)	114, 322, 814, 1610
MspR9I	CCNGG	8 cut(s)	262, 442, 469, 500, 647, 1441, 1643, 2487
Mva1269I	GAATGC	2 cut(s)	985, 1659
MvaI	CCWGG	2 cut(s)	1441, 1643
MvnI	CGCG	3 cut(s)	638, 820, 822
NaeI	GCCGGC	1 cut(s)	809
NarI	GGCGCC	1 cut(s)	378
NciI	CCSGG	6 cut(s)	262, 442, 469, 500, 647, 2487
NcoI	CCATGG	1 cut(s)	1274
NdeI	CATATG	3 cut(s)	1111, 1315, 1741
NgoMIV	GCCGGC	1 cut(s)	807
NheI	GCTAGC	1 cut(s)	1022
NmeAIII	GCCGAG	7 cut(s)	200, 406, 436, 470, 819, 828, 2326
NmuCI	GTSAC	5 cut(s)	25, 229, 334, 2289, 2429
NsiI	ATGCAT	1 cut(s)	2065
NspI	RCATGY	1 cut(s)	1547
PaeR7I	CTCGAG	1 cut(s)	344
PagI	TCATGA	2 cut(s)	273, 2100
PcsI	WCGNNNNNNNCGW	1 cut(s)	45
PctI	GAATGC	2 cut(s)	985, 1659
PdiI	GCCGGC	1 cut(s)	809
PdmI	GAANNNNTTC	1 cut(s)	2396
PfeI	GAWTC	5 cut(s)	667, 793, 1621, 2078, 2397
PflFI	GACNNNGTC	1 cut(s)	92
PfoI	TCCNGGA	3 cut(s)	467, 1439, 2485
PleI	GAGTC	2 cut(s)	1562, 1976
PluTI	GGCGCC	1 cut(s)	381
PmaCI	CACGTG	1 cut(s)	269
PmlI	CACGTG	1 cut(s)	269
PpsI	GAGTC	2 cut(s)	1562, 1976
Ppu21I	YACGTR	4 cut(s)	269, 591, 708, 1385
PshAI	GACNNNNGTC	2 cut(s)	254, 269
PsiI	TTATAA	1 cut(s)	1328
Psp124BI	GAGCTC	1 cut(s)	2533
Psp1406I	AACGTT	1 cut(s)	1342
Psp6I	CCWGG	2 cut(s)	1439, 1641
PspCI	CACGTG	1 cut(s)	269
PspEI	GGTNACC	2 cut(s)	25, 2429
PspFI	CCCAGC	2 cut(s)	406, 2152
PspGI	CCWGG	2 cut(s)	1439, 1641
PspPI	GGNCC	7 cut(s)	194, 461, 1304, 1615, 1693, 2298, 2477
PspXI	VCTCGAGB	1 cut(s)	344
PsrI	GAACNNNNNNTAC	2 cut(s)	987, 1019
PstI	CTGCAG	1 cut(s)	2427
PstNI	CAGNNNCTG	3 cut(s)	1772, 1960, 2549
PsuI	RGATCY	5 cut(s)	402, 562, 1528, 2156, 2320
PsyI	GACNNNGTC	1 cut(s)	92
PvuII	CAGCTG	1 cut(s)	1610
RsaI	GTAC	6 cut(s)	542, 629, 857, 1480, 2191, 2523
RsaNI	GTAC	6 cut(s)	541, 628, 856, 1479, 2190, 2522
RseI	CAYNNNNRTG	3 cut(s)	1404, 1410, 1744
SacI	GAGCTC	1 cut(s)	2533
SaqAI	TTAA	5 cut(s)	1206, 1515, 1802, 1821, 2085
Sau96I	GGNCC	7 cut(s)	194, 461, 1304, 1615, 1693, 2298, 2477
ScaI	AGTACT	1 cut(s)	2523
SchI	GAGTC	2 cut(s)	1562, 1976
ScrFI	CCNGG	8 cut(s)	262, 442, 469, 500, 647, 1441, 1643, 2487
SduI	GDGCHC	5 cut(s)	309, 435, 1188, 2307, 2533
SexAI	ACCWGGT	1 cut(s)	1641
SfaNI	GCATC	4 cut(s)	1047, 1402, 1443, 2072
SfcI	CTRYAG	2 cut(s)	1291, 2423
SfoI	GGCGCC	1 cut(s)	379
Sfr274I	CTCGAG	1 cut(s)	344
SinI	GGWCC	2 cut(s)	194, 461
SlaI	CTCGAG	1 cut(s)	344
SmiI	ATTTAAAT	1 cut(s)	2086
SmiMI	CAYNNNNRTG	3 cut(s)	1404, 1410, 1744
SmlI	CTYRAG	2 cut(s)	344, 1992
SmoI	CTYRAG	2 cut(s)	344, 1992
SnaBI	TACGTA	1 cut(s)	1385
Sse9I	AATT	6 cut(s)	506, 829, 997, 1425, 1818, 1902
SspDI	GGCGCC	1 cut(s)	377
SspMI	CTAG	1 cut(s)	1023
SstI	GAGCTC	1 cut(s)	2533
StyD4I	CCNGG	8 cut(s)	260, 440, 467, 498, 645, 1439, 1641, 2485
StyI	CCWWGG	5 cut(s)	1257, 1274, 2017, 2118, 2308
SwaI	ATTTAAAT	1 cut(s)	2086
TaaI	ACNGT	9 cut(s)	235, 256, 1194, 1213, 1292, 1362, 1520, 1843, 2545
TaiI	ACGT	8 cut(s)	17, 231, 271, 372, 593, 710, 1345, 1387
TaqI	TCGA	6 cut(s)	48, 63, 345, 504, 525, 2284
TasI	AATT	6 cut(s)	506, 829, 997, 1425, 1818, 1902
TatI	WGTACW	4 cut(s)	540, 855, 1478, 2521
TauI	GCSGC	7 cut(s)	114, 586, 790, 814, 820, 825, 2540
TfiI	GAWTC	5 cut(s)	667, 793, 1621, 2078, 2397
Tru1I	TTAA	5 cut(s)	1206, 1515, 1802, 1821, 2085
Tru9I	TTAA	5 cut(s)	1206, 1515, 1802, 1821, 2085
TscAI	CASTG	8 cut(s)	302, 647, 1216, 1295, 1367, 1584, 1965, 2206
TseFI	GTSAC	5 cut(s)	25, 229, 334, 2289, 2429
TseI	GCWGC	9 cut(s)	114, 655, 658, 814, 1310, 1607, 1889, 1972, 2243
Tsp45I	GTSAC	5 cut(s)	25, 229, 334, 2289, 2429
TspGWI	ACGGA	3 cut(s)	207, 607, 2250
TspRI	CASTG	8 cut(s)	302, 647, 1216, 1295, 1367, 1584, 1965, 2206
Tth111I	GACNNNGTC	1 cut(s)	92
VpaK11BI	GGWCC	2 cut(s)	194, 461
XapI	RAATTY	2 cut(s)	829, 1902
XceI	RCATGY	1 cut(s)	1547
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1272
XhoI	CTCGAG	1 cut(s)	344
XmnI	GAANNNNTTC	1 cut(s)	2396
XspI	CTAG	1 cut(s)	1023
ZraI	GACGTC	2 cut(s)	15, 370
ZrmI	AGTACT	1 cut(s)	2523
Zsp2I	ATGCAT	1 cut(s)	2065

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.