Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000855

Physical Location & Seq

Genomic Coordinates Reverse (-)

351269 .. 355311

Gene Structure 4043 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1006400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2493 bp

ATGGCGGCAGAAGAAGATAAATCTCAGACGATGGTTGTCTACCTCCACGGCGACCTTGATTTGAAAATTCTCGAGGCTCGATGTTTGCCCAACATGGATATGGTCTCGGAGCGCCTCCGCCGCTTCTTCTCGGCCTGTAGCAAGCCTTTTACACGCCCGGCGGCCCACCACTCCCACGGAAAAATCATCACCAGCGACCCCTACGTCACCGTCTGCCTCGCCGGTGCCACAGTGGCTCGCACCCGCGTCATTTCCAACTCCCAGAACCCCGTCTGGAAAGAGCATTTCAAGATCCCCCTGGCCCACCCCGTCTCGCAGGTCGAATTCTACGTCAAAGACAACGACATGTTTGGCGCAGATTTAATCGGCGTCGCGTCCGTCTCGGCGGTTAGAATCCTTTCCGGCGAAACGATCAGCGATTGGTTCCCGATCATCGGGCCGTTGGGCAAGCCGCCGAAGCCCGACGCGGCCGTTAAGCTCGAGATGCGGTTCACGAAATGCGAGGACAATCCCATGTACCGGCACGGTATTTCGAATGATCCAGACCACTTCGGGATCCGAAACTGTTATTTTCCGGTTCGGCAGGGAGGGCACGTGACGCTGTACCAGGACGCGCACGTGCCGGAGGCGATGCTGGAGGAGATTGAACTGGACGACGGCGTTACTTTTCAGCACGGGAGGTGCTGGGAGGATATCTGCCACGCGATATTGGAGGCGCATCATTTGGTTTATATTGTGGGATGGTCAATTTATCATCAGGTGAAGCTGGTGCGAGAACCGACGAAGCCGCTGCCGAGCGGCGGGAATTTGAACCTGGGGGAGCTGCTGAAGTACAAGTCCGAAGAAGGCTTGCGAGTTCTGCTGCTCGTTTGGGATGACAAGACTTCTCACAGCAAATTCTTCATCAATACGAATGGAGTGATGCAAACTCATGACGAAGAGACCCGCAAGTTCTTTAAGCACTCTTCGGTTTCATGCGTTGTTGGAACCCTGTTTACACATCACCAGAAATGTGTGATTGTGGATACACAAGCATCTGGAAATAACCGAAAGATAACCGCCTTCATAGGAGGTCTAGACCTTTGTGATGGCCGCTATGATACTCCTGAGCATCGCCTCTTTCGAGACCTCGACAGTGTTTTCCAGGATGATTATCATAATCCCACACTTTCTGGAGGAACAAAGGGTCCAAGGCAACCGTGGCATGATTTGCATTGCAAGATAGAAGGGCCGGCTGCCTATGATGTGCTTACCAATTTTGAGCAGCGTTGGAGAAAAGCCACAAAATGGTCGGAGTTGGGCCAACGATTCAAAAGAGTATCTCGCTGGCATGACGATGCTTTGATAAAATTGGAGCGCATCTCATGGATACTTAGTCCCTCCCCATCAACTGCCAAGGATGATCCCGCCTTATGGGTTTCAGATGAAAATGACCCTCAAAACTGGCATGTTCAGGTTTTTCGATCCATAGATTCTGGATCTCTGAAGGGATTTCCTAAGGATGTTTATAAAGCAGAGGCTCAGAATCTTGTTTGTGCCAAGAATTTGGTAGTAGACAAGAGCATACAAACAGCATACATCGATGCAATCCGATCTGCACAACACTTCATATACATTGAGAATCAGTATTTTGTCGGATCGTCGTATGGATGGCCAGCTTACAAAGAAGCAGGTGCTGACAATTTAATCCCGATGGAATTGGCATTAAAGATTGCGAGCAAGATAAGAGCAGACGAGAGATTCGCAGTTTATATCGTCATACCAATGTGGCCAGAGGGTGTTCCCTCCTCTGCTTCTGTGCAAGAAATTCTCTATTGGCAGGGACAGACAATGCAAATGATGTATGAAGTCGTAGCAAAAGAGCTGAAAGCCATGAAAGTGGAGAATGCTCATCCGCATGACTACCTAAACTTCTACTGTCTTGGAAATCGGGAGACACTGCCGGACACGCTATCCTGTCACGTGAATCAAACTGACAGAAACGGAGAATCTCACACGGTTTCGGCTTCCCAAAAGTTTCAACGGTTTATGATTTACGTACATGCCAAGGGAATGGTGGTAGATGATGAGTATGTGATCATAGGTTCCGCAAATATCAACCAACGATCTATGGCGGGTTCTAGAGACACCGAGATAGCCATGGGTGCATACCAGCCCCACCACACTTGGGGTAAGAAGAAGCGTCATCCTCGTGGCCAGGTGTATGGGTATAGAATGTCACTTTGGGCAGAACACTTGGGGATGGTGGACAAATGCTTCAAGGAGGCTGAAACGTTGGACTGTGTGAAGTGCGTGAACGCTATCGCTGGAGATAACTGGACGAGGTTTGTGGCGGATGACTTTGCACCATTACAAGGGCATTTGATGAAATACCCGGTTGCGGTAGATGTCAATGGGAAGGTAAGTGCAATTCCTGGACGAGAGAGTTTCCCTGACGTCGGTGGTAAGATCCTCGGAGGCCGAACTACCCTTCCTGATGCACTCACTACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006950 GO:0008081 GO:0008150 GO:0008152 GO:0008219 GO:0009266 GO:0009409 GO:0009506 GO:0009628 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010119 GO:0010646 GO:0010647 GO:0012501 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019637 GO:0023051 GO:0023056 GO:0030054 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046473 GO:0046486 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0090333 GO:1901419 GO:1901421 GO:1905957 GO:1905959

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

831

Amino Acids

93.8

Weight (kDa)

6.49

Isoelectric Point (pI)

28.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	18 - 143	1.9e-16	C2 domain
PLDc_2	PF13091	674 - 714	1.7e-06	PLD-like domain
PLDc	PF00614	678 - 703	2.5e-08	Phospholipase D Active site motif
PLD_C	PF12357	750 - 820	7.5e-26	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000855)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35790 AT4G35790 AT4G35790
fragaria_vesca	FvH4_1g11000 FvH4_1g11020 FvH4_1g11020 FvH4_2g37900 FvH4_5g01272 FvH4_5g28780 FvH4_5g28800 FvH4_7g08510 FvH4_7g08510
malus_domestica	MD02G1123100.v1.1 MD08G1149700.v1.1 MD08G1237200.v1.1 MD15G1006400.v1.1 MD15G1237100.v1.1
prunus_persica	Prupe.1G359000_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.7G175400_v2.0.a1
pyrus_communis	pycom02g09600 pycom15g00480 pycom15g21090
rosa_chinensis	RchiOBHm_Chr2g0098271 RchiOBHm_Chr6g0303711 RchiOBHm_Chr7g0221001 RchiOBHm_Chr7g0221011
rosa_laevigata	RLG00000002185 RLG00000011053 RLG00000016781
rosa_multiflora	Rmu_co8414259.1_g000001 Rmu_co8436567.1_g000002 Rmu_co8452585.1_g000001 Rmu_sc0001024.1_g000003 Rmu_sc0002105.1_g000010 Rmu_sc0002105.1_g000018 Rmu_sc0002105.1_g000020 Rmu_sc0009922.1_g000005 Rmu_sc0039265.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144090 Rroxscaffold_3G00238820 Rroxscaffold_7G00164460
rosa_rugosa	Rorug02G0071800 Rorug06G0324200 Rorug07G0199200 Rorug07G0199300 Rorug07G0199400 Rorug07G0199500 Rorug07G0199600
rosa_samantha	Rh1CG003000 Rh2AG118900 Rh2BG121800 Rh2CG123400 Rh2DG123500 Rh6AG438500 Rh6BG464500 Rh6CG450800 Rh6DG437500 Rh7AG339200 Rh7BG329900 Rh7CG357200 Rh7DG337900
rosa_wichuraiana	Rw2G009320 Rw6G037950 Rw7G028850

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1509
AarI	CACCTGC	1 cut(s)	1661
AasI	GACNNNNNNGTC	1 cut(s)	203
AatII	GACGTC	1 cut(s)	2439
Acc36I	ACCTGC	2 cut(s)	307, 1661
AccB1I	GGYRCC	1 cut(s)	224
AccB7I	CCANNNNNTGG	1 cut(s)	1287
AccBSI	CCGCTC	1 cut(s)	798
AccI	GTMKAC	2 cut(s)	39, 1554
AccII	CGCG	5 cut(s)	246, 374, 467, 614, 704
AclI	AACGTT	1 cut(s)	2273
AclWI	GGATC	9 cut(s)	286, 533, 550, 563, 1397, 1458, 1486, 1645, 2443
AcoI	YGGCCR	5 cut(s)	468, 1090, 1652, 1769, 2194
AcsI	RAATTY	6 cut(s)	66, 323, 805, 896, 1543, 1806
AcuI	CTGAAG	2 cut(s)	848, 1505
AcvI	CACGTG	3 cut(s)	595, 619, 1963
AcyI	GRCGYC	2 cut(s)	369, 2436
AfaI	GTAC	4 cut(s)	518, 605, 833, 2040
AfiI	CCNNNNNNNGG	9 cut(s)	434, 519, 800, 1287, 1413, 2167, 2354, 2380, 2438
AflIII	ACRYGT	1 cut(s)	345
AgsI	TTSAA	7 cut(s)	64, 289, 647, 811, 1312, 2021, 2260
AjnI	CCWGG	6 cut(s)	297, 606, 813, 1143, 2196, 2413
AjuI	GAANNNNNNNTTGG	2 cut(s)	2206, 2238
AloI	GAACNNNNNNTCC	4 cut(s)	1171, 1203, 2455, 2487
AluBI	AGCT	5 cut(s)	478, 766, 823, 1658, 1864
AluI	AGCT	5 cut(s)	478, 766, 823, 1658, 1864
Alw26I	GTCTC	7 cut(s)	109, 316, 385, 935, 1119, 1928, 2118
AlwI	GGATC	9 cut(s)	286, 533, 550, 563, 1397, 1458, 1486, 1645, 2443
AlwNI	CAGNNNCTG	1 cut(s)	1676
Ama87I	CYCGRG	2 cut(s)	71, 479
ApeKI	GCWGC	5 cut(s)	790, 823, 862, 1235, 1264
ApoI	RAATTY	6 cut(s)	66, 323, 805, 896, 1543, 1806
ArsI	GACNNNNNNTTYG	4 cut(s)	315, 347, 1724, 1756
Asp700I	GAANNNNTTC	1 cut(s)	397
AspLEI	GCGC	5 cut(s)	114, 356, 616, 718, 1359
AspS9I	GGNCC	6 cut(s)	163, 301, 437, 1187, 1229, 1300
AsuC2I	CCSGG	2 cut(s)	158, 2375
AsuHPI	GGTGA	4 cut(s)	181, 199, 772, 995
AsuII	TTCGAA	1 cut(s)	533
AvaI	CYCGRG	2 cut(s)	71, 479
AvaII	GGWCC	1 cut(s)	1187
AxyI	CCTNAGG	1 cut(s)	1497
BaeGI	GKGCMC	1 cut(s)	594
BalI	TGGCCA	3 cut(s)	1654, 1771, 2196
BamHI	GGATCC	1 cut(s)	555
BanI	GGYRCC	1 cut(s)	224
BauI	CACGAG	1 cut(s)	2190
BbrPI	CACGTG	3 cut(s)	595, 619, 1963
BbvI	GCAGC	5 cut(s)	777, 810, 849, 1222, 1276
BccI	CCATC	7 cut(s)	25, 735, 1082, 1393, 1644, 1687, 2236
BceAI	ACGGC	4 cut(s)	64, 424, 455, 673
BcgI	CGANNNNNNTGC	2 cut(s)	772, 806
BciT130I	CCWGG	6 cut(s)	299, 608, 815, 1145, 2198, 2415
BciVI	GTATCC	2 cut(s)	1018, 1362
BclI	TGATCA	1 cut(s)	2076
BcnI	CCSGG	2 cut(s)	158, 2375
BcoDI	GTCTC	7 cut(s)	109, 316, 385, 935, 1119, 1928, 2118
BfaI	CTAG	2 cut(s)	1076, 2121
BfmI	CTRYAG	1 cut(s)	136
BfoI	RGCGCY	1 cut(s)	115
BfuAI	ACCTGC	2 cut(s)	307, 1661
BfuI	GTATCC	2 cut(s)	1018, 1362
BglI	GCCNNNNNGGC	1 cut(s)	233
Bme1390I	CCNGG	8 cut(s)	158, 299, 608, 815, 1145, 2198, 2375, 2415
Bme18I	GGWCC	1 cut(s)	1187
BmeT110I	CYCGRG	2 cut(s)	71, 479
BmgT120I	GGNCC	6 cut(s)	163, 301, 437, 1187, 1229, 1300
BmiI	GGNNCC	6 cut(s)	226, 425, 557, 988, 1188, 2086
BmrFI	CCNGG	8 cut(s)	158, 299, 608, 815, 1145, 2198, 2375, 2415
BplI	GAGNNNNNCTC	2 cut(s)	1346, 1378
BpmI	CTGGAG	3 cut(s)	656, 1194, 2328
Bpu10I	CCTNAGC	1 cut(s)	1107
Bpu14I	TTCGAA	1 cut(s)	533
BpuMI	CCSGG	2 cut(s)	158, 2375
Bsa29I	ATCGAT	1 cut(s)	1581
BsaAI	YACGTR	4 cut(s)	595, 619, 1963, 2038
BsaBI	GATNNNNATC	1 cut(s)	1152
BsaHI	GRCGYC	2 cut(s)	369, 2436
BsaI	GGTCTC	3 cut(s)	109, 935, 1119
BsaWI	WCCGGW	1 cut(s)	574
BsaXI	ACNNNNNCTCC	2 cut(s)	579, 609
Bsc4I	CCNNNNNNNGG	9 cut(s)	434, 519, 800, 1287, 1413, 2167, 2354, 2380, 2438
Bse118I	RCCGGY	3 cut(s)	221, 519, 1231
Bse1I	ACTGG	3 cut(s)	654, 1448, 2321
Bse21I	CCTNAGG	1 cut(s)	1497
Bse3DI	GCAATG	1 cut(s)	1213
Bse8I	GATNNNNATC	1 cut(s)	1152
BseBI	CCWGG	6 cut(s)	299, 608, 815, 1145, 2198, 2415
BseCI	ATCGAT	1 cut(s)	1581
BseJI	GATNNNNATC	1 cut(s)	1152
BseLI	CCNNNNNNNGG	9 cut(s)	434, 519, 800, 1287, 1413, 2167, 2354, 2380, 2438
BseMI	GCAATG	1 cut(s)	1213
BseMII	CTCAG	3 cut(s)	38, 1098, 1535
BseNI	ACTGG	3 cut(s)	654, 1448, 2321
BseRI	GAGGAG	2 cut(s)	653, 1777
BseSI	GKGCMC	1 cut(s)	594
BseX3I	CGGCCG	1 cut(s)	468
BseXI	GCAGC	5 cut(s)	777, 810, 849, 1222, 1276
BseYI	CCCAGC	1 cut(s)	684
BsgI	GTGCAG	1 cut(s)	1581
Bsh1236I	CGCG	5 cut(s)	246, 374, 467, 614, 704
Bsh1285I	CGRYCG	1 cut(s)	471
BshNI	GGYRCC	1 cut(s)	224
BshVI	ATCGAT	1 cut(s)	1581
BsiEI	CGRYCG	1 cut(s)	471
BsiHKCI	CYCGRG	2 cut(s)	71, 479
BsiSI	CCGG	9 cut(s)	158, 222, 402, 520, 575, 623, 1232, 1943, 2375
BslFI	GGGAC	2 cut(s)	1362, 1836
BslI	CCNNNNNNNGG	9 cut(s)	434, 519, 800, 1287, 1413, 2167, 2354, 2380, 2438
BsmAI	GTCTC	7 cut(s)	109, 316, 385, 935, 1119, 1928, 2118
BsmBI	CGTCTC	2 cut(s)	316, 385
BsmFI	GGGAC	2 cut(s)	1362, 1836
BsmI	GAATGC	1 cut(s)	1891
Bso31I	GGTCTC	3 cut(s)	109, 935, 1119
BsoBI	CYCGRG	2 cut(s)	71, 479
Bsp119I	TTCGAA	1 cut(s)	533
Bsp1286I	GDGCHC	1 cut(s)	594
Bsp19I	CCATGG	1 cut(s)	2139
BspCNI	CTCAG	3 cut(s)	37, 1099, 1534
BspDI	ATCGAT	1 cut(s)	1581
BspFNI	CGCG	5 cut(s)	246, 374, 467, 614, 704
BspHI	TCATGA	1 cut(s)	931
BspLI	GGNNCC	6 cut(s)	226, 425, 557, 988, 1188, 2086
BspMI	ACCTGC	2 cut(s)	307, 1661
BspPI	GGATC	9 cut(s)	286, 533, 550, 563, 1397, 1458, 1486, 1645, 2443
BspT104I	TTCGAA	1 cut(s)	533
BspT107I	GGYRCC	1 cut(s)	224
BspTNI	GGTCTC	3 cut(s)	109, 935, 1119
BsrBI	CCGCTC	1 cut(s)	798
BsrDI	GCAATG	1 cut(s)	1213
BsrFI	RCCGGY	3 cut(s)	221, 519, 1231
BsrI	ACTGG	3 cut(s)	654, 1448, 2321
BssAI	RCCGGY	3 cut(s)	221, 519, 1231
BssNI	GRCGYC	2 cut(s)	369, 2436
BssSI	CACGAG	1 cut(s)	2190
BssT1I	CCWWGG	4 cut(s)	1190, 1395, 2046, 2139
Bst2BI	CACGAG	1 cut(s)	2190
Bst2UI	CCWGG	6 cut(s)	299, 608, 815, 1145, 2198, 2415
Bst6I	CTCTTC	2 cut(s)	933, 970
BstACI	GRCGYC	2 cut(s)	369, 2436
BstAPI	GCANNNNNTGC	1 cut(s)	1210
BstBAI	YACGTR	4 cut(s)	595, 619, 1963, 2038
BstBI	TTCGAA	1 cut(s)	533
BstC8I	GCNNGC	8 cut(s)	143, 238, 449, 851, 1233, 1328, 1656, 1717
BstDEI	CTNAG	5 cut(s)	24, 1107, 1373, 1497, 1521
BstDSI	CCRYGG	4 cut(s)	46, 175, 1199, 2139
BstFNI	CGCG	5 cut(s)	246, 374, 467, 614, 704
BstH2I	RGCGCY	1 cut(s)	115
BstHHI	GCGC	5 cut(s)	114, 356, 616, 718, 1359
BstMAI	GTCTC	7 cut(s)	109, 316, 385, 935, 1119, 1928, 2118
BstMCI	CGRYCG	1 cut(s)	471
BstNI	CCWGG	6 cut(s)	299, 608, 815, 1145, 2198, 2415
BstNSI	RCATGY	3 cut(s)	349, 1451, 2045
BstSCI	CCNGG	8 cut(s)	156, 297, 606, 813, 1143, 2196, 2373, 2413
BstSFI	CTRYAG	1 cut(s)	136
BstSLI	GKGCMC	1 cut(s)	594
BstSNI	TACGTA	1 cut(s)	2038
BstUI	CGCG	5 cut(s)	246, 374, 467, 614, 704
BstV1I	GCAGC	5 cut(s)	777, 810, 849, 1222, 1276
BstX2I	RGATCY	4 cut(s)	291, 555, 1478, 2448
BstXI	CCANNNNNNTGG	4 cut(s)	1546, 1879, 2053, 2204
BstYI	RGATCY	4 cut(s)	291, 555, 1478, 2448
BstZI	CGGCCG	1 cut(s)	468
Bsu15I	ATCGAT	1 cut(s)	1581
Bsu36I	CCTNAGG	1 cut(s)	1497
BsuI	GTATCC	2 cut(s)	1018, 1362
BsuTUI	ATCGAT	1 cut(s)	1581
BtgI	CCRYGG	4 cut(s)	46, 175, 1199, 2139
BtgZI	GCGATG	2 cut(s)	644, 1097
BtsI	GCAGTG	1 cut(s)	1937
BtsIMutI	CAGTG	3 cut(s)	237, 1141, 1937
BveI	ACCTGC	2 cut(s)	307, 1661
Cac8I	GCNNGC	8 cut(s)	143, 238, 449, 851, 1233, 1328, 1656, 1717
CaiI	CAGNNNCTG	1 cut(s)	1676
CciI	TCATGA	1 cut(s)	931
CfoI	GCGC	5 cut(s)	114, 356, 616, 718, 1359
Cfr10I	RCCGGY	3 cut(s)	221, 519, 1231
Cfr13I	GGNCC	6 cut(s)	163, 301, 437, 1187, 1229, 1300
ClaI	ATCGAT	1 cut(s)	1581
CseI	GACGC	7 cut(s)	235, 358, 363, 473, 607, 620, 2171
Csp6I	GTAC	4 cut(s)	517, 604, 832, 2039
CspCI	CAANNNNNGTGG	2 cut(s)	2147, 2182
CviQI	GTAC	4 cut(s)	517, 604, 832, 2039
DdeI	CTNAG	5 cut(s)	24, 1107, 1373, 1497, 1521
DrdI	GACNNNNNNGTC	1 cut(s)	203
DseDI	GACNNNNNNGTC	1 cut(s)	203
EaeI	YGGCCR	5 cut(s)	468, 1090, 1652, 1769, 2194
EagI	CGGCCG	1 cut(s)	468
Eam1104I	CTCTTC	2 cut(s)	933, 970
EarI	CTCTTC	2 cut(s)	933, 970
EciI	GGCGGA	2 cut(s)	107, 2348
EclXI	CGGCCG	1 cut(s)	468
Eco105I	TACGTA	1 cut(s)	2038
Eco130I	CCWWGG	4 cut(s)	1190, 1395, 2046, 2139
Eco31I	GGTCTC	3 cut(s)	109, 935, 1119
Eco32I	GATATC	1 cut(s)	694
Eco47I	GGWCC	1 cut(s)	1187
Eco52I	CGGCCG	1 cut(s)	468
Eco57I	CTGAAG	2 cut(s)	848, 1505
Eco72I	CACGTG	3 cut(s)	595, 619, 1963
Eco81I	CCTNAGG	1 cut(s)	1497
Eco88I	CYCGRG	2 cut(s)	71, 479
EcoRI	GAATTC	1 cut(s)	323
EcoRII	CCWGG	6 cut(s)	297, 606, 813, 1143, 2196, 2413
EcoRV	GATATC	1 cut(s)	694
EcoT14I	CCWWGG	4 cut(s)	1190, 1395, 2046, 2139
ErhI	CCWWGG	4 cut(s)	1190, 1395, 2046, 2139
Esp3I	CGTCTC	2 cut(s)	316, 385
FaqI	GGGAC	2 cut(s)	1362, 1836
FauI	CCCGC	5 cut(s)	251, 794, 953, 1414, 2107
FbaI	TGATCA	1 cut(s)	2076
FblI	GTMKAC	2 cut(s)	39, 1554
FspBI	CTAG	2 cut(s)	1076, 2121
GlaI	GCGC	5 cut(s)	113, 355, 615, 717, 1358
GsaI	CCCAGC	1 cut(s)	688
GsuI	CTGGAG	3 cut(s)	656, 1194, 2328
HaeII	RGCGCY	1 cut(s)	115
HapII	CCGG	9 cut(s)	158, 222, 402, 520, 575, 623, 1232, 1943, 2375
HgaI	GACGC	7 cut(s)	235, 358, 363, 473, 607, 620, 2171
HhaI	GCGC	5 cut(s)	114, 356, 616, 718, 1359
Hin1I	GRCGYC	2 cut(s)	369, 2436
Hin6I	GCGC	5 cut(s)	112, 354, 614, 716, 1357
HinP1I	GCGC	5 cut(s)	112, 354, 614, 716, 1357
HinfI	GANTC	8 cut(s)	393, 1308, 1472, 1525, 1621, 1740, 1966, 1988
HpaII	CCGG	9 cut(s)	158, 222, 402, 520, 575, 623, 1232, 1943, 2375
HphI	GGTGA	4 cut(s)	181, 199, 772, 995
Hpy166II	GTNNAC	6 cut(s)	40, 492, 996, 1555, 2248, 2296
Hpy8I	GTNNAC	6 cut(s)	40, 492, 996, 1555, 2248, 2296
Hpy99I	CGWCG	6 cut(s)	374, 467, 659, 784, 1645, 2441
HpyAV	CCTTC	6 cut(s)	839, 1072, 1220, 1480, 2392, 2480
HpyCH4IV	ACGT	8 cut(s)	204, 330, 594, 618, 1962, 2037, 2273, 2436
HpyF3I	CTNAG	5 cut(s)	24, 1107, 1373, 1497, 1521
HpySE526I	ACGT	8 cut(s)	204, 330, 594, 618, 1962, 2037, 2273, 2436
Hsp92I	GRCGYC	2 cut(s)	369, 2436
HspAI	GCGC	5 cut(s)	112, 354, 614, 716, 1357
KroI	GCCGGC	1 cut(s)	1231
KroNI	GCCGGC	1 cut(s)	1233
Ksp22I	TGATCA	1 cut(s)	2076
LmnI	GCTCC	3 cut(s)	109, 820, 1354
Lsp1109I	GCAGC	5 cut(s)	777, 810, 849, 1222, 1276
MaeI	CTAG	2 cut(s)	1076, 2121
MaeII	ACGT	8 cut(s)	204, 330, 594, 618, 1962, 2037, 2273, 2436
MaeIII	GTNAC	5 cut(s)	205, 595, 661, 1958, 2217
MbiI	CCGCTC	1 cut(s)	798
MboII	GAAGA	8 cut(s)	23, 26, 118, 854, 892, 950, 957, 2188
MflI	RGATCY	4 cut(s)	291, 555, 1478, 2448
MhlI	GDGCHC	1 cut(s)	594
MlsI	TGGCCA	3 cut(s)	1654, 1771, 2196
MluNI	TGGCCA	3 cut(s)	1654, 1771, 2196
MmeI	TCCRAC	6 cut(s)	279, 964, 1250, 1272, 1615, 2256
Mox20I	TGGCCA	3 cut(s)	1654, 1771, 2196
MroNI	GCCGGC	1 cut(s)	1231
MroXI	GAANNNNTTC	1 cut(s)	397
MscI	TGGCCA	3 cut(s)	1654, 1771, 2196
MseI	TTAA	6 cut(s)	362, 474, 957, 1685, 1706, 2491
MslI	CAYNNNNRTG	6 cut(s)	98, 1335, 1763, 1877, 1896, 2190
Msp20I	TGGCCA	3 cut(s)	1654, 1771, 2196
MspA1I	CMGCKG	1 cut(s)	790
MspI	CCGG	9 cut(s)	158, 222, 402, 520, 575, 623, 1232, 1943, 2375
MspR9I	CCNGG	8 cut(s)	158, 299, 608, 815, 1145, 2198, 2375, 2415
Mva1269I	GAATGC	1 cut(s)	1891
MvaI	CCWGG	6 cut(s)	299, 608, 815, 1145, 2198, 2415
MvnI	CGCG	5 cut(s)	246, 374, 467, 614, 704
NaeI	GCCGGC	1 cut(s)	1233
NciI	CCSGG	2 cut(s)	158, 2375
NcoI	CCATGG	1 cut(s)	2139
NgoMIV	GCCGGC	1 cut(s)	1231
NlaIV	GGNNCC	6 cut(s)	226, 425, 557, 988, 1188, 2086
NmeAIII	GCCGAG	3 cut(s)	110, 362, 819
NmuCI	GTSAC	4 cut(s)	205, 595, 1958, 2217
NspI	RCATGY	3 cut(s)	349, 1451, 2045
NspV	TTCGAA	1 cut(s)	533
PaeR7I	CTCGAG	2 cut(s)	71, 479
PagI	TCATGA	1 cut(s)	931
PaqCI	CACCTGC	1 cut(s)	1661
PciI	ACATGT	1 cut(s)	345
PcsI	WCGNNNNNNNCGW	2 cut(s)	327, 1120
PctI	GAATGC	1 cut(s)	1891
PdiI	GCCGGC	1 cut(s)	1233
PdmI	GAANNNNTTC	1 cut(s)	397
PfeI	GAWTC	8 cut(s)	393, 1308, 1472, 1525, 1621, 1740, 1966, 1988
PflMI	CCANNNNNTGG	1 cut(s)	1287
PfoI	TCCNGGA	2 cut(s)	1143, 2413
PmaCI	CACGTG	3 cut(s)	595, 619, 1963
PmlI	CACGTG	3 cut(s)	595, 619, 1963
Ppu21I	YACGTR	4 cut(s)	595, 619, 1963, 2038
PscI	ACATGT	1 cut(s)	345
PsiI	TTATAA	1 cut(s)	1509
Psp1406I	AACGTT	1 cut(s)	2273
Psp6I	CCWGG	6 cut(s)	297, 606, 813, 1143, 2196, 2413
PspCI	CACGTG	3 cut(s)	595, 619, 1963
PspFI	CCCAGC	1 cut(s)	684
PspGI	CCWGG	6 cut(s)	297, 606, 813, 1143, 2196, 2413
PspN4I	GGNNCC	6 cut(s)	226, 425, 557, 988, 1188, 2086
PspPI	GGNCC	6 cut(s)	163, 301, 437, 1187, 1229, 1300
PsrI	GAACNNNNNNTAC	2 cut(s)	979, 1011
PstNI	CAGNNNCTG	1 cut(s)	1676
PsuI	RGATCY	4 cut(s)	291, 555, 1478, 2448
RsaI	GTAC	4 cut(s)	518, 605, 833, 2040
RsaNI	GTAC	4 cut(s)	517, 604, 832, 2039
RseI	CAYNNNNRTG	6 cut(s)	98, 1335, 1763, 1877, 1896, 2190
SaqAI	TTAA	6 cut(s)	362, 474, 957, 1685, 1706, 2491
Sau96I	GGNCC	6 cut(s)	163, 301, 437, 1187, 1229, 1300
ScrFI	CCNGG	8 cut(s)	158, 299, 608, 815, 1145, 2198, 2375, 2415
SduI	GDGCHC	1 cut(s)	594
SfcI	CTRYAG	1 cut(s)	136
Sfr274I	CTCGAG	2 cut(s)	71, 479
SfuI	TTCGAA	1 cut(s)	533
SgrAI	CRCCGGYG	1 cut(s)	221
SinI	GGWCC	1 cut(s)	1187
SlaI	CTCGAG	2 cut(s)	71, 479
SmiMI	CAYNNNNRTG	6 cut(s)	98, 1335, 1763, 1877, 1896, 2190
SmlI	CTYRAG	2 cut(s)	71, 479
SmoI	CTYRAG	2 cut(s)	71, 479
SnaBI	TACGTA	1 cut(s)	2038
SspMI	CTAG	2 cut(s)	1076, 2121
StyD4I	CCNGG	8 cut(s)	156, 297, 606, 813, 1143, 2196, 2373, 2413
StyI	CCWWGG	4 cut(s)	1190, 1395, 2046, 2139
TaiI	ACGT	8 cut(s)	207, 333, 597, 621, 1965, 2040, 2276, 2439
TaqI	TCGA	9 cut(s)	72, 79, 321, 480, 533, 1123, 1131, 1462, 1581
TatI	WGTACW	1 cut(s)	831
TauI	GCSGC	8 cut(s)	8, 123, 164, 454, 470, 790, 801, 1095
TfiI	GAWTC	8 cut(s)	393, 1308, 1472, 1525, 1621, 1740, 1966, 1988
Tru1I	TTAA	6 cut(s)	362, 474, 957, 1685, 1706, 2491
Tru9I	TTAA	6 cut(s)	362, 474, 957, 1685, 1706, 2491
TscAI	CASTG	3 cut(s)	237, 1141, 1944
TseFI	GTSAC	4 cut(s)	205, 595, 1958, 2217
TseI	GCWGC	5 cut(s)	790, 823, 862, 1235, 1264
Tsp45I	GTSAC	4 cut(s)	205, 595, 1958, 2217
TspDTI	ATGAA	8 cut(s)	892, 963, 1054, 1440, 1597, 1860, 1889, 2381
TspGWI	ACGGA	3 cut(s)	192, 367, 1998
TspRI	CASTG	3 cut(s)	237, 1141, 1944
Van91I	CCANNNNNTGG	1 cut(s)	1287
VpaK11BI	GGWCC	1 cut(s)	1187
XapI	RAATTY	6 cut(s)	66, 323, 805, 896, 1543, 1806
XbaI	TCTAGA	2 cut(s)	1075, 2120
XceI	RCATGY	3 cut(s)	349, 1451, 2045
XcmI	CCANNNNNNNNNTGG	3 cut(s)	97, 1197, 2053
XhoI	CTCGAG	2 cut(s)	71, 479
XmiI	GTMKAC	2 cut(s)	39, 1554
XmnI	GAANNNNTTC	1 cut(s)	397
XspI	CTAG	2 cut(s)	1076, 2121
ZraI	GACGTC	1 cut(s)	2437

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.