Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0000855

Physical Location & Seq

Genomic Coordinates Forward (+)

30468462 .. 30473410

Gene Structure 4949 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1237200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2538 bp

ATGGGTAGCGACGGCAGTGAGGATGGGAGTCCTAAAGAACATATTATCTTGCATGGTGAACTGGATTTGTGGATTATAGAAGCTAAAGCTCTTCCAAATATGGATTTACCTTCGGAAAGAATGAGACGGTGTTTCACCATGTTTGGGACTTGCTCTACTCCCTTCGGTAATAGGCCAACTAAAACAGCTTCGGGAAAGCAGGCAATGATTACAAGTGATCCTTATGTGTCTGTTTGCCTAGCCGGAGCAACTGTTGCTCAAACCAGAATAATTTCAAACTGCGAGAATCCTTCGTGGGAAGAACATTTTTGCGTACCTGTTGCTCACCCTGTTGTGAAGGTGGAGTTTCATGTCAAAGATAACGATGTTCTTGGAGCAGAACTTATTGGAGTAGTGGAGATATCTGTTGATAAAATCATTTCGGGAAAGCCAATTAATGATTGGTTTCCGATTATTGGCAATCATGGGAACTGTTTAAAACCTTTTCCTGAACTGCATGTTTCTATTCAGTTTAAGCCAGTTGAAGGTAACCCTTTGTATAAGAATGGTGTTGGCGCTGGGCCCGACTATAAAGGAGTTCCTAATACATATTTCCCCCTTCGCAAAGGAGGGAGTGTCACTTTATATCAAGATGCTCATGTACCTAACAATATGCTGCCAGAAATTGTACTGGAAGGAGGGAAAATTTTTCAGCAAGGGAAGTGCTGGGAAGACATATGTCATGCCATCTTGGAAGCTCAGCACCTTATATATATTATAGGCTGGTCAGTGTACCACCGTGTTAAGCTTGTAAGGGAGCCAACAAAGCCACTACCTTCTGGAGGAGAGCTGGCACTTGGGGAGTTGTTGAAGTACAAGTCACAAGAAGGGATTCGTGTTGTTATGCTGATCTGGGATGATAAAACTTCACATGACAAATTATTTTTGAAAACGGAAGGTGTCATGCAGACCCATGACGAGGAAACCAAAAGGTTTTTCAAACATTCCAGTGTTCATTGTGTTCTTTCTCCTCGTTATGCGAGCAATAAGCTTAGTATTTTCAAGCAACAGGTTGTGGGAACCCTTTTTACGCATCATCAGAAATGTGTGCTTCTTGACACAGCTTCTGGGAATAATCGGAAAATAACTGCTTTCATTGGTGGCCTGGATATGTGTGACGGAAGATATGACACTCCGGAGCACAGGCTCTTTAAAGATGTCAACTCAGTCTTTCAAAATGATATTCATAATCCTACATTCCCTCCTAACGATCATGGCCCGAGGGAACCATGGCATGATTTACACTGTAAAATTGAGGGTCCTGCTGCATATGATATATTGACAAATTTTGAACAAAGATGGAGAAAGGCAAAAAAGCGCGACTTCAAGATAAAAAAGGTCACTAATAGGCATGATGATGCCTTGATAAGGCTTGATCGCGTTTCATGGATAGTCTCTCCAACTTCTAATCGTGATGGTGAACAAAATGTACGTGTTAGCAGTGAAGAAGATCGTGAGAACTGGCATGTTCAGGTATTCAGGTCCATTGACTCAGGATCTGTAAAGGGATTTCCGAAGGGCGTCCAGGATGCTGAAGCGCAGAATCTTATTTGTGGAAAGAATTTGAAAATAGATAAGAGCATCCATGTTGCATATATTAAAGCTATACGATCAGCTCAACGCTTTATATACATAGAGAATCAGTACTTTGTTGGATCTTCATTCTACTGGCCATCGTACAAAAATGCAGGTGCAGATAACTTGATCCCGATGGAACTGGCCCTAAAAGTTGCCAGCAAAATCAAAGCAAATGAGCGTTTCTCGGTTTATATAGTAGTACCTATGTGGCCAGAGGGTGTCCCAACTGCCTCTGCTACTCAGGAAATTTTATACTGGCAGGCACAGACAATGGGTATGATGTACCAGGTTGTTACAAGTGCCCTTCAAGATGCAGGCCTTTTCGATCAGTATCATCCACAGGATTATTTGAACTTTTACTGCCTCGGTAACCGTGAAGCTCCATCTTCATCCCAACCTCATCAGCCAACCGATAATCGTGGACTGTTGTTAGCGCAAAAGTTCCGGCGGTTTATGATTTATGTTCATGCCAAAGGGATGATAGTTGATGATGAGTATGTAATCTTGGGATCTGCAAACATTAACCAAAGATCAATGGACGGTTCAAGGGACACAGAAATTGCGATGGGTGCTTACCAACCACACCATACATGGGCTGCAGAAAAGAATCTTCATCCACACGGCCAGGTATATGGTTATCGGATGTCACTGTGGGCTGAGCACCTGGGCGGAGTTGAGGATACCTATCAAGACCCCGAGAGCCTTGAATGCGTGAAGCGTGTTAACGACATCGCGAAGCTTAACTGGAAAGCATTTGTGTCTGAAGAGCATAGGGAGATGAAGGGACACTTGATGACATACCCAGTTCAGATTGGGAGAGATGGACGAGTGAGTTCGTTGCCTGGATACGAATCGTTTCCTGATGTTGGTGGTAAAATATTGGGAGCACCCACCAGTCTTCCGGATGCCCTAACAACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006950 GO:0008081 GO:0008150 GO:0008152 GO:0008219 GO:0009266 GO:0009409 GO:0009506 GO:0009628 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010119 GO:0010646 GO:0010647 GO:0012501 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019637 GO:0023051 GO:0023056 GO:0030054 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046473 GO:0046486 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0090333 GO:1901419 GO:1901421 GO:1905957 GO:1905959

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

846

Amino Acids

95.3

Weight (kDa)

6.66

Isoelectric Point (pI)

33.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	20 - 150	1.8e-16	C2 domain
PLDc_2	PF13091	625 - 728	9.7e-07	PLD-like domain
PLDc	PF00614	692 - 717	9.3e-08	Phospholipase D Active site motif
PLD_C	PF12357	765 - 835	5.4e-29	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000855)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35790 AT4G35790 AT4G35790
fragaria_vesca	FvH4_1g11000 FvH4_1g11020 FvH4_1g11020 FvH4_2g37900 FvH4_5g01272 FvH4_5g28780 FvH4_5g28800 FvH4_7g08510 FvH4_7g08510
malus_domestica	MD02G1123100.v1.1 MD08G1149700.v1.1 MD08G1237200.v1.1 MD15G1006400.v1.1 MD15G1237100.v1.1
prunus_persica	Prupe.1G359000_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.1G574700_v2.0.a1 Prupe.7G175400_v2.0.a1
pyrus_communis	pycom02g09600 pycom15g00480 pycom15g21090
rosa_chinensis	RchiOBHm_Chr2g0098271 RchiOBHm_Chr6g0303711 RchiOBHm_Chr7g0221001 RchiOBHm_Chr7g0221011
rosa_laevigata	RLG00000002185 RLG00000011053 RLG00000016781
rosa_multiflora	Rmu_co8414259.1_g000001 Rmu_co8436567.1_g000002 Rmu_co8452585.1_g000001 Rmu_sc0001024.1_g000003 Rmu_sc0002105.1_g000010 Rmu_sc0002105.1_g000018 Rmu_sc0002105.1_g000020 Rmu_sc0009922.1_g000005 Rmu_sc0039265.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144090 Rroxscaffold_3G00238820 Rroxscaffold_7G00164460
rosa_rugosa	Rorug02G0071800 Rorug06G0324200 Rorug07G0199200 Rorug07G0199300 Rorug07G0199400 Rorug07G0199500 Rorug07G0199600
rosa_samantha	Rh1CG003000 Rh2AG118900 Rh2BG121800 Rh2CG123400 Rh2DG123500 Rh6AG438500 Rh6BG464500 Rh6CG450800 Rh6DG437500 Rh7AG339200 Rh7BG329900 Rh7CG357200 Rh7DG337900
rosa_wichuraiana	Rw2G009320 Rw6G037950 Rw7G028850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1718
Acc36I	ACCTGC	1 cut(s)	1718
AccII	CGCG	3 cut(s)	1359, 1419, 2351
AccIII	TCCGGA	2 cut(s)	1174, 2518
AciI	CCGC	2 cut(s)	2065, 2286
AclWI	GGATC	5 cut(s)	212, 1543, 1702, 1738, 2134
AcoI	YGGCCR	3 cut(s)	1709, 1826, 2239
AcsI	RAATTY	4 cut(s)	684, 1324, 1600, 1863
AcuI	CTGAAG	2 cut(s)	1593, 2400
AcyI	GRCGYC	1 cut(s)	1560
AfiI	CCNNNNNNNGG	8 cut(s)	144, 455, 524, 821, 958, 1407, 2209, 2483
AflIII	ACRYGT	1 cut(s)	1471
AjnI	CCWGG	6 cut(s)	1143, 1563, 1902, 2241, 2280, 2458
AjuI	GAANNNNNNNTTGG	2 cut(s)	959, 991
Alw21I	GWGCWC	3 cut(s)	1182, 2280, 2506
Alw26I	GTCTC	2 cut(s)	118, 1438
AlwI	GGATC	5 cut(s)	212, 1543, 1702, 1738, 2134
AlwNI	CAGNNNCTG	2 cut(s)	1106, 1538
Ama87I	CYCGRG	2 cut(s)	1258, 2312
Aor13HI	TCCGGA	2 cut(s)	1174, 2518
AoxI	GGCC	9 cut(s)	173, 560, 1141, 1255, 1709, 1758, 1826, 1933, 2239
ApaI	GGGCCC	1 cut(s)	564
ApeKI	GCWGC	3 cut(s)	655, 1304, 2213
ApoI	RAATTY	4 cut(s)	684, 1324, 1600, 1863
AseI	ATTAAT	1 cut(s)	435
Asp700I	GAANNNNTTC	3 cut(s)	271, 870, 2472
AspLEI	GCGC	4 cut(s)	557, 1359, 1579, 2053
AspS9I	GGNCC	6 cut(s)	560, 561, 1256, 1298, 1521, 1759
AsuHPI	GGTGA	4 cut(s)	68, 127, 317, 1469
AvaI	CYCGRG	2 cut(s)	1258, 2312
AvaII	GGWCC	2 cut(s)	1298, 1521
BaeGI	GKGCMC	2 cut(s)	564, 1921
BalI	TGGCCA	2 cut(s)	1711, 1828
BanII	GRGCYC	1 cut(s)	564
BbsI	GAAGAC	2 cut(s)	717, 2507
Bbv12I	GWGCWC	3 cut(s)	1182, 2280, 2506
BbvI	GCAGC	3 cut(s)	642, 1291, 2200
BccI	CCATC	9 cut(s)	17, 734, 1332, 1448, 1720, 1744, 2008, 2176, 2432
BceAI	ACGGC	2 cut(s)	28, 2254
BciT130I	CCWGG	6 cut(s)	1145, 1565, 1904, 2243, 2282, 2460
BciVI	GTATCC	2 cut(s)	2290, 2456
BcoDI	GTCTC	2 cut(s)	118, 1438
BfaI	CTAG	1 cut(s)	239
BfmI	CTRYAG	1 cut(s)	2214
BfoI	RGCGCY	1 cut(s)	558
BfuAI	ACCTGC	1 cut(s)	1718
BfuI	GTATCC	2 cut(s)	2290, 2456
BisI	GCNGC	3 cut(s)	656, 1305, 2214
BlpI	GCTNAGC	2 cut(s)	738, 2274
BlsI	GCNGC	3 cut(s)	657, 1306, 2215
BmcAI	AGTACT	1 cut(s)	1685
Bme1390I	CCNGG	6 cut(s)	1145, 1565, 1904, 2243, 2282, 2460
Bme18I	GGWCC	2 cut(s)	1298, 1521
BmeT110I	CYCGRG	2 cut(s)	1258, 2312
BmgT120I	GGNCC	6 cut(s)	560, 561, 1256, 1298, 1521, 1759
BmiI	GGNNCC	5 cut(s)	562, 798, 1060, 1266, 1299
BmrFI	CCNGG	6 cut(s)	1145, 1565, 1904, 2243, 2282, 2460
BmrI	ACTGGG	1 cut(s)	2414
BmsI	GCATC	7 cut(s)	622, 1081, 1387, 1558, 1629, 1918, 2512
BmuI	ACTGGG	1 cut(s)	2414
BoxI	GACNNNNGTC	1 cut(s)	717
BpiI	GAAGAC	2 cut(s)	717, 2507
BplI	GAGNNNNNCTC	2 cut(s)	1784, 1816
BpmI	CTGGAG	1 cut(s)	840
Bpu1102I	GCTNAGC	2 cut(s)	738, 2274
BsaAI	YACGTR	1 cut(s)	1472
BsaBI	GATNNNNATC	3 cut(s)	1947, 2301, 2467
BsaHI	GRCGYC	1 cut(s)	1560
BsaJI	CCNNGG	4 cut(s)	1259, 1268, 1981, 2281
BsaWI	WCCGGW	2 cut(s)	1174, 2518
BsaXI	ACNNNNNCTCC	6 cut(s)	237, 267, 600, 630, 1225, 1255
Bsc4I	CCNNNNNNNGG	8 cut(s)	144, 455, 524, 821, 958, 1407, 2209, 2483
Bse3DI	GCAATG	1 cut(s)	210
Bse8I	GATNNNNATC	3 cut(s)	1947, 2301, 2467
BseAI	TCCGGA	2 cut(s)	1174, 2518
BseBI	CCWGG	6 cut(s)	1145, 1565, 1904, 2243, 2282, 2460
BseDI	CCNNGG	4 cut(s)	1259, 1268, 1981, 2281
BseJI	GATNNNNATC	3 cut(s)	1947, 2301, 2467
BseLI	CCNNNNNNNGG	8 cut(s)	144, 455, 524, 821, 958, 1407, 2209, 2483
BseMI	GCAATG	1 cut(s)	210
BseMII	CTCAG	5 cut(s)	752, 1218, 1545, 1871, 2265
BseRI	GAGGAG	2 cut(s)	837, 999
BseSI	GKGCMC	2 cut(s)	564, 1921
BseXI	GCAGC	3 cut(s)	642, 1291, 2200
BseYI	CCCAGC	2 cut(s)	557, 705
BsgI	GTGCAG	1 cut(s)	1752
Bsh1236I	CGCG	3 cut(s)	1359, 1419, 2351
BshFI	GGCC	9 cut(s)	175, 562, 1143, 1257, 1711, 1760, 1828, 1935, 2241
BsiHKAI	GWGCWC	3 cut(s)	1182, 2280, 2506
BsiHKCI	CYCGRG	2 cut(s)	1258, 2312
BsiSI	CCGG	4 cut(s)	243, 1175, 2062, 2519
BslFI	GGGAC	4 cut(s)	160, 1823, 2180, 2415
BslI	CCNNNNNNNGG	8 cut(s)	144, 455, 524, 821, 958, 1407, 2209, 2483
BsmAI	GTCTC	2 cut(s)	118, 1438
BsmBI	CGTCTC	1 cut(s)	118
BsmFI	GGGAC	4 cut(s)	160, 1823, 2180, 2415
BsmI	GAATGC	1 cut(s)	2330
BsnI	GGCC	9 cut(s)	175, 562, 1143, 1257, 1711, 1760, 1828, 1935, 2241
BsoBI	CYCGRG	2 cut(s)	1258, 2312
Bsp120I	GGGCCC	1 cut(s)	560
Bsp1286I	GDGCHC	5 cut(s)	564, 1182, 1921, 2280, 2506
Bsp13I	TCCGGA	2 cut(s)	1174, 2518
Bsp1720I	GCTNAGC	2 cut(s)	738, 2274
Bsp19I	CCATGG	1 cut(s)	1268
Bsp68I	TCGCGA	1 cut(s)	2351
BspACI	CCGC	2 cut(s)	2065, 2286
BspANI	GGCC	9 cut(s)	175, 562, 1143, 1257, 1711, 1760, 1828, 1935, 2241
BspCNI	CTCAG	5 cut(s)	751, 1217, 1544, 1870, 2266
BspEI	TCCGGA	2 cut(s)	1174, 2518
BspFNI	CGCG	3 cut(s)	1359, 1419, 2351
BspLI	GGNNCC	5 cut(s)	562, 798, 1060, 1266, 1299
BspMAI	CTGCAG	1 cut(s)	2218
BspMI	ACCTGC	1 cut(s)	1718
BspPI	GGATC	5 cut(s)	212, 1543, 1702, 1738, 2134
BspQI	GCTCTTC	2 cut(s)	96, 2376
BsrDI	GCAATG	1 cut(s)	210
BssECI	CCNNGG	4 cut(s)	1259, 1268, 1981, 2281
BssNI	GRCGYC	1 cut(s)	1560
BssT1I	CCWWGG	1 cut(s)	1268
Bst2UI	CCWGG	6 cut(s)	1145, 1565, 1904, 2243, 2282, 2460
Bst4CI	ACNGT	9 cut(s)	129, 253, 473, 779, 1286, 1991, 2043, 2159, 2268
Bst6I	CTCTTC	2 cut(s)	96, 2376
BstACI	GRCGYC	1 cut(s)	1560
BstAPI	GCANNNNNTGC	1 cut(s)	254
BstBAI	YACGTR	1 cut(s)	1472
BstC8I	GCNNGC	6 cut(s)	201, 831, 1021, 1774, 1878, 1933
BstDEI	CTNAG	6 cut(s)	738, 1031, 1204, 1531, 1857, 2274
BstDSI	CCRYGG	1 cut(s)	1268
BstEII	GGTNACC	2 cut(s)	527, 1985
BstENI	CCTNNNNNAGG	2 cut(s)	819, 1405
BstFNI	CGCG	3 cut(s)	1359, 1419, 2351
BstH2I	RGCGCY	1 cut(s)	558
BstHHI	GCGC	4 cut(s)	557, 1359, 1579, 2053
BstMAI	GTCTC	2 cut(s)	118, 1438
BstMWI	GCNNNNNNNGC	4 cut(s)	254, 805, 2186, 2325
BstNI	CCWGG	6 cut(s)	1145, 1565, 1904, 2243, 2282, 2460
BstNSI	RCATGY	2 cut(s)	500, 1508
BstPAI	GACNNNNGTC	1 cut(s)	717
BstPI	GGTNACC	2 cut(s)	527, 1985
BstSCI	CCNGG	6 cut(s)	1143, 1563, 1902, 2241, 2280, 2458
BstSFI	CTRYAG	1 cut(s)	2214
BstSLI	GKGCMC	2 cut(s)	564, 1921
BstUI	CGCG	3 cut(s)	1359, 1419, 2351
BstV1I	GCAGC	3 cut(s)	642, 1291, 2200
BstV2I	GAAGAC	2 cut(s)	717, 2507
BstX2I	RGATCY	3 cut(s)	1535, 1694, 2126
BstXI	CCANNNNNNTGG	1 cut(s)	2249
BstYI	RGATCY	3 cut(s)	1535, 1694, 2126
BsuI	GTATCC	2 cut(s)	2290, 2456
BsuRI	GGCC	9 cut(s)	175, 562, 1143, 1257, 1711, 1760, 1828, 1935, 2241
BtgI	CCRYGG	1 cut(s)	1268
BtgZI	GCGATG	2 cut(s)	2195, 2332
BtsI	GCAGTG	2 cut(s)	22, 1486
BtsIMutI	CAGTG	6 cut(s)	22, 774, 994, 1282, 1486, 2264
BtuMI	TCGCGA	1 cut(s)	2351
BveI	ACCTGC	1 cut(s)	1718
Cac8I	GCNNGC	6 cut(s)	201, 831, 1021, 1774, 1878, 1933
CaiI	CAGNNNCTG	2 cut(s)	1106, 1538
CfoI	GCGC	4 cut(s)	557, 1359, 1579, 2053
Cfr13I	GGNCC	6 cut(s)	560, 561, 1256, 1298, 1521, 1759
CseI	GACGC	1 cut(s)	1549
CsiI	ACCWGGT	1 cut(s)	1902
DdeI	CTNAG	6 cut(s)	738, 1031, 1204, 1531, 1857, 2274
DraI	TTTAAA	2 cut(s)	477, 1192
EaeI	YGGCCR	3 cut(s)	1709, 1826, 2239
Eam1104I	CTCTTC	2 cut(s)	96, 2376
EarI	CTCTTC	2 cut(s)	96, 2376
EciI	GGCGGA	1 cut(s)	2301
Eco130I	CCWWGG	1 cut(s)	1268
Eco147I	AGGCCT	1 cut(s)	1935
Eco24I	GRGCYC	1 cut(s)	564
Eco32I	GATATC	1 cut(s)	402
Eco47I	GGWCC	2 cut(s)	1298, 1521
Eco57I	CTGAAG	2 cut(s)	1593, 2400
Eco88I	CYCGRG	2 cut(s)	1258, 2312
Eco91I	GGTNACC	2 cut(s)	527, 1985
EcoNI	CCTNNNNNAGG	2 cut(s)	819, 1405
EcoO109I	RGGNCCY	1 cut(s)	1298
EcoO65I	GGTNACC	2 cut(s)	527, 1985
EcoRII	CCWGG	6 cut(s)	1143, 1563, 1902, 2241, 2280, 2458
EcoRV	GATATC	1 cut(s)	402
EcoT14I	CCWWGG	1 cut(s)	1268
EcoT38I	GRGCYC	1 cut(s)	564
ErhI	CCWWGG	1 cut(s)	1268
Esp3I	CGTCTC	1 cut(s)	118
FalI	AAGNNNNNCTT	6 cut(s)	205, 237, 1346, 1378, 2390, 2422
FaqI	GGGAC	4 cut(s)	160, 1823, 2180, 2415
FauNDI	CATATG	2 cut(s)	716, 1309
Fnu4HI	GCNGC	3 cut(s)	656, 1305, 2214
FriOI	GRGCYC	1 cut(s)	564
Fsp4HI	GCNGC	3 cut(s)	656, 1305, 2214
FspBI	CTAG	1 cut(s)	239
GlaI	GCGC	4 cut(s)	556, 1358, 1578, 2052
GluI	GCNGC	3 cut(s)	656, 1305, 2214
GsaI	CCCAGC	2 cut(s)	561, 709
GsuI	CTGGAG	1 cut(s)	840
HaeII	RGCGCY	1 cut(s)	558
HaeIII	GGCC	9 cut(s)	175, 562, 1143, 1257, 1711, 1760, 1828, 1935, 2241
HapII	CCGG	4 cut(s)	243, 1175, 2062, 2519
HgaI	GACGC	1 cut(s)	1549
HhaI	GCGC	4 cut(s)	557, 1359, 1579, 2053
Hin1I	GRCGYC	1 cut(s)	1560
Hin6I	GCGC	4 cut(s)	555, 1357, 1577, 2051
HinP1I	GCGC	4 cut(s)	555, 1357, 1577, 2051
HincII	GTYRAC	2 cut(s)	1201, 2341
HindII	GTYRAC	2 cut(s)	1201, 2341
HindIII	AAGCTT	3 cut(s)	785, 1028, 2354
HinfI	GANTC	8 cut(s)	28, 286, 871, 1529, 1582, 1678, 2224, 2468
HpaI	GTTAAC	1 cut(s)	2341
HpaII	CCGG	4 cut(s)	243, 1175, 2062, 2519
HphI	GGTGA	4 cut(s)	68, 127, 317, 1469
Hpy166II	GTNNAC	6 cut(s)	59, 772, 1201, 1460, 2039, 2341
Hpy188I	TCNGA	8 cut(s)	115, 450, 1080, 1119, 1554, 2259, 2380, 2427
Hpy8I	GTNNAC	6 cut(s)	59, 772, 1201, 1460, 2039, 2341
Hpy99I	CGWCG	1 cut(s)	14
HpyCH4III	ACNGT	9 cut(s)	129, 253, 473, 779, 1286, 1991, 2043, 2159, 2268
HpyCH4IV	ACGT	1 cut(s)	1471
HpyF10VI	GCNNNNNNNGC	4 cut(s)	254, 805, 2186, 2325
HpyF3I	CTNAG	6 cut(s)	738, 1031, 1204, 1531, 1857, 2274
HpySE526I	ACGT	1 cut(s)	1471
Hsp92I	GRCGYC	1 cut(s)	1560
HspAI	GCGC	4 cut(s)	555, 1357, 1577, 2051
Kpn2I	TCCGGA	2 cut(s)	1174, 2518
KspAI	GTTAAC	1 cut(s)	2341
LguI	GCTCTTC	2 cut(s)	96, 2376
LmnI	GCTCC	6 cut(s)	245, 374, 796, 1177, 2002, 2501
Lsp1109I	GCAGC	3 cut(s)	642, 1291, 2200
LweI	GCATC	7 cut(s)	622, 1081, 1387, 1558, 1629, 1918, 2512
MabI	ACCWGGT	1 cut(s)	1902
MaeI	CTAG	1 cut(s)	239
MaeII	ACGT	1 cut(s)	1471
MaeIII	GTNAC	8 cut(s)	527, 616, 858, 1154, 1378, 1909, 1985, 2262
MflI	RGATCY	3 cut(s)	1535, 1694, 2126
MhlI	GDGCHC	5 cut(s)	564, 1182, 1921, 2280, 2506
MlsI	TGGCCA	2 cut(s)	1711, 1828
MluNI	TGGCCA	2 cut(s)	1711, 1828
MlyI	GAGTC	2 cut(s)	37, 1523
MmeI	TCCRAC	2 cut(s)	1463, 1672
Mox20I	TGGCCA	2 cut(s)	1711, 1828
MroI	TCCGGA	2 cut(s)	1174, 2518
MroXI	GAANNNNTTC	3 cut(s)	271, 870, 2472
MscI	TGGCCA	2 cut(s)	1711, 1828
MseI	TTAA	9 cut(s)	435, 476, 513, 783, 1191, 1638, 2139, 2340, 2358
MslI	CAYNNNNRTG	2 cut(s)	987, 1395
Msp20I	TGGCCA	2 cut(s)	1711, 1828
MspI	CCGG	4 cut(s)	243, 1175, 2062, 2519
MspR9I	CCNGG	6 cut(s)	1145, 1565, 1904, 2243, 2282, 2460
Mva1269I	GAATGC	1 cut(s)	2330
MvaI	CCWGG	6 cut(s)	1145, 1565, 1904, 2243, 2282, 2460
MvnI	CGCG	3 cut(s)	1359, 1419, 2351
MwoI	GCNNNNNNNGC	4 cut(s)	254, 805, 2186, 2325
NcoI	CCATGG	1 cut(s)	1268
NdeI	CATATG	2 cut(s)	716, 1309
NlaIV	GGNNCC	5 cut(s)	562, 798, 1060, 1266, 1299
NmuCI	GTSAC	5 cut(s)	616, 858, 1154, 1378, 2262
NruI	TCGCGA	1 cut(s)	2351
NspI	RCATGY	2 cut(s)	500, 1508
PaqCI	CACCTGC	1 cut(s)	1718
PceI	AGGCCT	1 cut(s)	1935
PciSI	GCTCTTC	2 cut(s)	96, 2376
PctI	GAATGC	1 cut(s)	2330
PdmI	GAANNNNTTC	3 cut(s)	271, 870, 2472
PfeI	GAWTC	6 cut(s)	286, 871, 1582, 1678, 2224, 2468
PfoI	TCCNGGA	1 cut(s)	1563
PkrI	GCNGC	3 cut(s)	657, 1306, 2215
PleI	GAGTC	2 cut(s)	36, 1523
PpsI	GAGTC	2 cut(s)	36, 1523
Ppu21I	YACGTR	1 cut(s)	1472
PpuMI	RGGWCCY	1 cut(s)	1298
PshAI	GACNNNNGTC	1 cut(s)	717
PshBI	ATTAAT	1 cut(s)	435
Psp5II	RGGWCCY	1 cut(s)	1298
Psp6I	CCWGG	6 cut(s)	1143, 1563, 1902, 2241, 2280, 2458
PspEI	GGTNACC	2 cut(s)	527, 1985
PspFI	CCCAGC	2 cut(s)	557, 705
PspGI	CCWGG	6 cut(s)	1143, 1563, 1902, 2241, 2280, 2458
PspN4I	GGNNCC	5 cut(s)	562, 798, 1060, 1266, 1299
PspOMI	GGGCCC	1 cut(s)	560
PspPI	GGNCC	6 cut(s)	560, 561, 1256, 1298, 1521, 1759
PspPPI	RGGWCCY	1 cut(s)	1298
PsrI	GAACNNNNNNTAC	4 cut(s)	1051, 1083, 1452, 1484
PstI	CTGCAG	1 cut(s)	2218
PstNI	CAGNNNCTG	2 cut(s)	1106, 1538
PsuI	RGATCY	3 cut(s)	1535, 1694, 2126
RruI	TCGCGA	1 cut(s)	2351
RseI	CAYNNNNRTG	2 cut(s)	987, 1395
SapI	GCTCTTC	2 cut(s)	96, 2376
SaqAI	TTAA	9 cut(s)	435, 476, 513, 783, 1191, 1638, 2139, 2340, 2358
SatI	GCNGC	3 cut(s)	656, 1305, 2214
Sau96I	GGNCC	6 cut(s)	560, 561, 1256, 1298, 1521, 1759
ScaI	AGTACT	1 cut(s)	1685
SchI	GAGTC	2 cut(s)	37, 1523
ScrFI	CCNGG	6 cut(s)	1145, 1565, 1904, 2243, 2282, 2460
SduI	GDGCHC	5 cut(s)	564, 1182, 1921, 2280, 2506
SexAI	ACCWGGT	1 cut(s)	1902
SfaNI	GCATC	7 cut(s)	622, 1081, 1387, 1558, 1629, 1918, 2512
SfcI	CTRYAG	1 cut(s)	2214
SinI	GGWCC	2 cut(s)	1298, 1521
SmiMI	CAYNNNNRTG	2 cut(s)	987, 1395
SseBI	AGGCCT	1 cut(s)	1935
SsiI	CCGC	2 cut(s)	2065, 2286
SspI	AATATT	1 cut(s)	2496
SspMI	CTAG	1 cut(s)	239
StuI	AGGCCT	1 cut(s)	1935
StyD4I	CCNGG	6 cut(s)	1143, 1563, 1902, 2241, 2280, 2458
StyI	CCWWGG	1 cut(s)	1268
TaaI	ACNGT	9 cut(s)	129, 253, 473, 779, 1286, 1991, 2043, 2159, 2268
TaiI	ACGT	1 cut(s)	1474
TaqI	TCGA	1 cut(s)	1941
TatI	WGTACW	3 cut(s)	667, 852, 1683
TfiI	GAWTC	6 cut(s)	286, 871, 1582, 1678, 2224, 2468
Tru1I	TTAA	9 cut(s)	435, 476, 513, 783, 1191, 1638, 2139, 2340, 2358
Tru9I	TTAA	9 cut(s)	435, 476, 513, 783, 1191, 1638, 2139, 2340, 2358
TscAI	CASTG	6 cut(s)	22, 774, 994, 1289, 1486, 2271
TseFI	GTSAC	5 cut(s)	616, 858, 1154, 1378, 2262
TseI	GCWGC	3 cut(s)	655, 1304, 2213
Tsp45I	GTSAC	5 cut(s)	616, 858, 1154, 1378, 2262
TspGWI	ACGGA	2 cut(s)	947, 1173
TspRI	CASTG	6 cut(s)	22, 774, 994, 1289, 1486, 2271
VpaK11BI	GGWCC	2 cut(s)	1298, 1521
VspI	ATTAAT	1 cut(s)	435
XagI	CCTNNNNNAGG	2 cut(s)	819, 1405
XapI	RAATTY	4 cut(s)	684, 1324, 1600, 1863
XceI	RCATGY	2 cut(s)	500, 1508
XcmI	CCANNNNNNNNNTGG	2 cut(s)	438, 2205
XmnI	GAANNNNTTC	3 cut(s)	271, 870, 2472
XspI	CTAG	1 cut(s)	239
ZrmI	AGTACT	1 cut(s)	1685

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.