Phospholipase D
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 1346, 1631, 2061 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 533 |
| AatII | GACGTC | 1 cut(s) | 678 |
| Acc36I | ACCTGC | 1 cut(s) | 348 |
| AccB1I | GGYRCC | 1 cut(s) | 154 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 304 |
| AccBSI | CCGCTC | 4 cut(s) | 53, 328, 907, 2566 |
| AccII | CGCG | 4 cut(s) | 944, 1123, 1125, 1663 |
| AccIII | TCCGGA | 2 cut(s) | 871, 909 |
| AclI | AACGTT | 1 cut(s) | 1645 |
| AcoI | YGGCCR | 4 cut(s) | 306, 1112, 1903, 2051 |
| AcsI | RAATTY | 2 cut(s) | 2205, 2847 |
| AcuI | CTGAAG | 1 cut(s) | 2193 |
| AcvI | CACGTG | 1 cut(s) | 575 |
| AcyI | GRCGYC | 3 cut(s) | 155, 214, 675 |
| AdeI | CACNNNGTG | 1 cut(s) | 1087 |
| AfeI | AGCGCT | 1 cut(s) | 410 |
| AflIII | ACRYGT | 4 cut(s) | 396, 572, 574, 894 |
| AgsI | TTSAA | 4 cut(s) | 1234, 1285, 1773, 1967 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 1207, 2365 |
| AjiI | CACGTC | 1 cut(s) | 661 |
| AjnI | CCWGG | 3 cut(s) | 952, 1742, 1980 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 219, 251, 2581, 2613 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1549, 1581 |
| AluBI | AGCT | 9 cut(s) | 235, 739, 921, 1336, 1901, 1973, 2075, 2129, 2513 |
| AluI | AGCT | 9 cut(s) | 235, 739, 921, 1336, 1901, 1973, 2075, 2129, 2513 |
| Alw21I | GWGCWC | 4 cut(s) | 615, 741, 879, 1491 |
| Alw26I | GTCTC | 2 cut(s) | 92, 187 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2075 |
| Ama87I | CYCGRG | 2 cut(s) | 650, 1484 |
| Aor13HI | TCCGGA | 2 cut(s) | 871, 909 |
| Aor51HI | AGCGCT | 1 cut(s) | 410 |
| AoxI | GGCC | 9 cut(s) | 306, 1112, 1607, 1903, 1917, 2051, 2168, 2601, 2779 |
| ApeKI | GCWGC | 6 cut(s) | 420, 961, 1117, 1613, 1910, 2546 |
| ApoI | RAATTY | 2 cut(s) | 2205, 2847 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 662, 694 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 1280, 2337, 2699 |
| AspLEI | GCGC | 7 cut(s) | 157, 411, 634, 686, 946, 1045, 1125 |
| AspS9I | GGNCC | 6 cut(s) | 547, 767, 1607, 1863, 2601, 2780 |
| AsuC2I | CCSGG | 6 cut(s) | 568, 713, 748, 775, 1273, 2330 |
| AsuHPI | GGTGA | 7 cut(s) | 418, 511, 529, 1366, 1538, 2189, 2726 |
| AsuII | TTCGAA | 2 cut(s) | 369, 2634 |
| AsuNHI | GCTAGC | 1 cut(s) | 1325 |
| AvaI | CYCGRG | 2 cut(s) | 650, 1484 |
| AvaII | GGWCC | 3 cut(s) | 547, 767, 1863 |
| AxyI | CCTNAGG | 1 cut(s) | 2343 |
| BalI | TGGCCA | 1 cut(s) | 2053 |
| BamHI | GGATCC | 1 cut(s) | 868 |
| BanI | GGYRCC | 1 cut(s) | 154 |
| BanII | GRGCYC | 2 cut(s) | 741, 2288 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2615, 2647 |
| BauI | CACGAG | 1 cut(s) | 159 |
| BbrPI | CACGTG | 1 cut(s) | 575 |
| BbsI | GAAGAC | 3 cut(s) | 477, 1803, 2361 |
| Bbv12I | GWGCWC | 4 cut(s) | 615, 741, 879, 1491 |
| BbvCI | CCTCAGC | 1 cut(s) | 1914 |
| BbvI | GCAGC | 6 cut(s) | 407, 948, 1104, 1600, 1897, 2533 |
| BccI | CCATC | 8 cut(s) | 449, 1243, 1389, 1409, 1460, 1598, 1771, 2762 |
| BceAI | ACGGC | 4 cut(s) | 361, 811, 1254, 1890 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2250, 2284 |
| BciT130I | CCWGG | 3 cut(s) | 954, 1744, 1982 |
| BciVI | GTATCC | 4 cut(s) | 1740, 2341, 2634, 2757 |
| BcnI | CCSGG | 6 cut(s) | 568, 713, 748, 775, 1273, 2330 |
| BcoDI | GTCTC | 2 cut(s) | 92, 187 |
| BfaI | CTAG | 1 cut(s) | 1326 |
| BfmI | CTRYAG | 3 cut(s) | 1594, 2726, 2786 |
| BfoI | RGCGCY | 3 cut(s) | 158, 412, 687 |
| BfuAI | ACCTGC | 1 cut(s) | 348 |
| BfuI | GTATCC | 4 cut(s) | 1740, 2341, 2634, 2757 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1111 |
| BlpI | GCTNAGC | 1 cut(s) | 1337 |
| BmcAI | AGTACT | 1 cut(s) | 2248 |
| Bme1390I | CCNGG | 9 cut(s) | 568, 713, 748, 775, 954, 1273, 1744, 1982, 2330 |
| Bme18I | GGWCC | 3 cut(s) | 547, 767, 1863 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 1207, 2365 |
| BmeT110I | CYCGRG | 2 cut(s) | 650, 1484 |
| BmgBI | CACGTC | 1 cut(s) | 661 |
| BmgT120I | GGNCC | 6 cut(s) | 547, 767, 1607, 1863, 2601, 2780 |
| BmrFI | CCNGG | 9 cut(s) | 568, 713, 748, 775, 954, 1273, 1744, 1982, 2330 |
| BmrI | ACTGGG | 1 cut(s) | 836 |
| BmsI | GCATC | 9 cut(s) | 502, 1054, 1128, 1705, 1746, 1933, 2353, 2605, 2617 |
| BmtI | GCTAGC | 1 cut(s) | 1329 |
| BmuI | ACTGGG | 1 cut(s) | 836 |
| BoxI | GACNNNNGTC | 2 cut(s) | 560, 575 |
| BpiI | GAAGAC | 3 cut(s) | 477, 1803, 2361 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 728, 760 |
| BpmI | CTGGAG | 2 cut(s) | 975, 2694 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1914 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1337 |
| Bpu14I | TTCGAA | 2 cut(s) | 369, 2634 |
| BpuEI | CTTGAG | 2 cut(s) | 1139, 2280 |
| BpuMI | CCSGG | 6 cut(s) | 568, 713, 748, 775, 1273, 2330 |
| BsaAI | YACGTR | 4 cut(s) | 575, 897, 2449, 2758 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1533, 2704 |
| BsaHI | GRCGYC | 3 cut(s) | 155, 214, 675 |
| BsaJI | CCNNGG | 6 cut(s) | 150, 952, 1560, 1577, 2320, 2421 |
| BsaWI | WCCGGW | 4 cut(s) | 731, 871, 909, 1860 |
| Bse118I | RCCGGY | 1 cut(s) | 1110 |
| Bse1I | ACTGG | 2 cut(s) | 842, 2696 |
| Bse21I | CCTNAGG | 1 cut(s) | 2343 |
| Bse3DI | GCAATG | 2 cut(s) | 2202, 2244 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1533, 2704 |
| BseAI | TCCGGA | 2 cut(s) | 871, 909 |
| BseBI | CCWGG | 3 cut(s) | 954, 1744, 1982 |
| BseDI | CCNNGG | 6 cut(s) | 150, 952, 1560, 1577, 2320, 2421 |
| BseGI | GGATG | 6 cut(s) | 46, 65, 336, 1977, 2290, 2560 |
| BseJI | GATNNNNATC | 2 cut(s) | 1533, 2704 |
| BseMI | GCAATG | 2 cut(s) | 2202, 2244 |
| BseMII | CTCAG | 7 cut(s) | 678, 1676, 1887, 1905, 2334, 2496, 2634 |
| BseNI | ACTGG | 2 cut(s) | 842, 2696 |
| BseRI | GAGGAG | 4 cut(s) | 175, 338, 442, 1184 |
| BseX3I | CGGCCG | 1 cut(s) | 1112 |
| BseXI | GCAGC | 6 cut(s) | 407, 948, 1104, 1600, 1897, 2533 |
| BseYI | CCCAGC | 2 cut(s) | 231, 266 |
| BsgI | GTGCAG | 1 cut(s) | 326 |
| Bsh1236I | CGCG | 4 cut(s) | 944, 1123, 1125, 1663 |
| Bsh1285I | CGRYCG | 2 cut(s) | 1115, 2778 |
| BshFI | GGCC | 9 cut(s) | 308, 1114, 1609, 1905, 1919, 2053, 2170, 2603, 2781 |
| BshNI | GGYRCC | 1 cut(s) | 154 |
| BsiEI | CGRYCG | 2 cut(s) | 1115, 2778 |
| BsiHKAI | GWGCWC | 4 cut(s) | 615, 741, 879, 1491 |
| BsiHKCI | CYCGRG | 2 cut(s) | 650, 1484 |
| BslFI | GGGAC | 6 cut(s) | 533, 608, 1214, 1267, 2785, 2805 |
| BsmAI | GTCTC | 2 cut(s) | 92, 187 |
| BsmBI | CGTCTC | 2 cut(s) | 92, 187 |
| BsmFI | GGGAC | 6 cut(s) | 533, 608, 1214, 1267, 2785, 2805 |
| BsmI | GAATGC | 2 cut(s) | 1288, 1962 |
| BsnI | GGCC | 9 cut(s) | 308, 1114, 1609, 1905, 1919, 2053, 2170, 2603, 2781 |
| BsoBI | CYCGRG | 2 cut(s) | 650, 1484 |
| Bsp119I | TTCGAA | 2 cut(s) | 369, 2634 |
| Bsp1286I | GDGCHC | 5 cut(s) | 615, 741, 879, 1491, 2288 |
| Bsp13I | TCCGGA | 2 cut(s) | 871, 909 |
| Bsp1407I | TGTACA | 3 cut(s) | 284, 846, 993 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1337 |
| Bsp19I | CCATGG | 1 cut(s) | 1577 |
| BspANI | GGCC | 9 cut(s) | 308, 1114, 1609, 1905, 1919, 2053, 2170, 2603, 2781 |
| BspCNI | CTCAG | 7 cut(s) | 679, 1677, 1886, 1906, 2335, 2497, 2635 |
| BspEI | TCCGGA | 2 cut(s) | 871, 909 |
| BspFNI | CGCG | 4 cut(s) | 944, 1123, 1125, 1663 |
| BspHI | TCATGA | 1 cut(s) | 579 |
| BspMAI | CTGCAG | 2 cut(s) | 2730, 2790 |
| BspMI | ACCTGC | 1 cut(s) | 348 |
| BspOI | GCTAGC | 1 cut(s) | 1329 |
| BspQI | GCTCTTC | 1 cut(s) | 2631 |
| BspT104I | TTCGAA | 2 cut(s) | 369, 2634 |
| BspT107I | GGYRCC | 1 cut(s) | 154 |
| BsrBI | CCGCTC | 4 cut(s) | 53, 328, 907, 2566 |
| BsrDI | GCAATG | 2 cut(s) | 2202, 2244 |
| BsrFI | RCCGGY | 1 cut(s) | 1110 |
| BsrGI | TGTACA | 3 cut(s) | 284, 846, 993 |
| BsrI | ACTGG | 2 cut(s) | 842, 2696 |
| BssAI | RCCGGY | 1 cut(s) | 1110 |
| BssECI | CCNNGG | 6 cut(s) | 150, 952, 1560, 1577, 2320, 2421 |
| BssNI | GRCGYC | 3 cut(s) | 155, 214, 675 |
| BssSI | CACGAG | 1 cut(s) | 159 |
| BssT1I | CCWWGG | 4 cut(s) | 1560, 1577, 2320, 2421 |
| Bst2BI | CACGAG | 1 cut(s) | 159 |
| Bst2UI | CCWGG | 3 cut(s) | 954, 1744, 1982 |
| Bst6I | CTCTTC | 3 cut(s) | 2216, 2631, 2690 |
| BstACI | GRCGYC | 3 cut(s) | 155, 214, 675 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1588, 2230 |
| BstAUI | TGTACA | 3 cut(s) | 284, 846, 993 |
| BstBAI | YACGTR | 4 cut(s) | 575, 897, 2449, 2758 |
| BstBI | TTCGAA | 2 cut(s) | 369, 2634 |
| BstDSI | CCRYGG | 1 cut(s) | 1577 |
| BstEII | GGTNACC | 2 cut(s) | 2324, 2732 |
| BstF5I | GGATG | 6 cut(s) | 46, 65, 336, 1977, 2290, 2560 |
| BstFNI | CGCG | 4 cut(s) | 944, 1123, 1125, 1663 |
| BstH2I | RGCGCY | 3 cut(s) | 158, 412, 687 |
| BstHHI | GCGC | 7 cut(s) | 157, 411, 634, 686, 946, 1045, 1125 |
| BstMAI | GTCTC | 2 cut(s) | 92, 187 |
| BstMCI | CGRYCG | 2 cut(s) | 1115, 2778 |
| BstNI | CCWGG | 3 cut(s) | 954, 1744, 1982 |
| BstNSI | RCATGY | 2 cut(s) | 400, 1850 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 560, 575 |
| BstPI | GGTNACC | 2 cut(s) | 2324, 2732 |
| BstSCI | CCNGG | 9 cut(s) | 566, 711, 746, 773, 952, 1271, 1742, 1980, 2328 |
| BstSFI | CTRYAG | 3 cut(s) | 1594, 2726, 2786 |
| BstSNI | TACGTA | 1 cut(s) | 2449 |
| BstUI | CGCG | 4 cut(s) | 944, 1123, 1125, 1663 |
| BstV1I | GCAGC | 6 cut(s) | 407, 948, 1104, 1600, 1897, 2533 |
| BstV2I | GAAGAC | 3 cut(s) | 477, 1803, 2361 |
| BstX2I | RGATCY | 6 cut(s) | 708, 868, 1831, 1991, 2459, 2831 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2428 |
| BstYI | RGATCY | 6 cut(s) | 708, 868, 1831, 1991, 2459, 2831 |
| BstZI | CGGCCG | 1 cut(s) | 1112 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2343 |
| BsuI | GTATCC | 4 cut(s) | 1740, 2341, 2634, 2757 |
| BsuRI | GGCC | 9 cut(s) | 308, 1114, 1609, 1905, 1919, 2053, 2170, 2603, 2781 |
| BtgI | CCRYGG | 1 cut(s) | 1577 |
| BtgZI | GCGATG | 1 cut(s) | 1836 |
| BtrI | CACGTC | 1 cut(s) | 661 |
| BtsCI | GGATG | 6 cut(s) | 46, 65, 336, 1977, 2290, 2560 |
| BtsI | GCAGTG | 3 cut(s) | 287, 608, 946 |
| BtsIMutI | CAGTG | 9 cut(s) | 287, 608, 849, 946, 1512, 1591, 1880, 2261, 2502 |
| BveI | ACCTGC | 1 cut(s) | 348 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2075 |
| CciI | TCATGA | 1 cut(s) | 579 |
| CfoI | GCGC | 7 cut(s) | 157, 411, 634, 686, 946, 1045, 1125 |
| Cfr10I | RCCGGY | 1 cut(s) | 1110 |
| Cfr13I | GGNCC | 6 cut(s) | 547, 767, 1607, 1863, 2601, 2780 |
| CseI | GACGC | 4 cut(s) | 222, 950, 1264, 2355 |
| DinI | GGCGCC | 1 cut(s) | 156 |
| DraI | TTTAAA | 1 cut(s) | 126 |
| DraIII | CACNNNGTG | 1 cut(s) | 1087 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 533 |
| DriI | GACNNNNNGTC | 2 cut(s) | 1207, 2365 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 533 |
| EaeI | YGGCCR | 4 cut(s) | 306, 1112, 1903, 2051 |
| EagI | CGGCCG | 1 cut(s) | 1112 |
| Eam1104I | CTCTTC | 3 cut(s) | 2216, 2631, 2690 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 1207, 2365 |
| EarI | CTCTTC | 3 cut(s) | 2216, 2631, 2690 |
| EciI | GGCGGA | 6 cut(s) | 404, 454, 495, 731, 1016, 1984 |
| Ecl136II | GAGCTC | 1 cut(s) | 739 |
| EclXI | CGGCCG | 1 cut(s) | 1112 |
| Eco105I | TACGTA | 1 cut(s) | 2449 |
| Eco130I | CCWWGG | 4 cut(s) | 1560, 1577, 2320, 2421 |
| Eco24I | GRGCYC | 2 cut(s) | 741, 2288 |
| Eco32I | GATATC | 1 cut(s) | 2585 |
| Eco47I | GGWCC | 3 cut(s) | 547, 767, 1863 |
| Eco47III | AGCGCT | 1 cut(s) | 410 |
| Eco52I | CGGCCG | 1 cut(s) | 1112 |
| Eco53kI | GAGCTC | 1 cut(s) | 739 |
| Eco57I | CTGAAG | 1 cut(s) | 2193 |
| Eco72I | CACGTG | 1 cut(s) | 575 |
| Eco81I | CCTNAGG | 1 cut(s) | 2343 |
| Eco88I | CYCGRG | 2 cut(s) | 650, 1484 |
| Eco91I | GGTNACC | 2 cut(s) | 2324, 2732 |
| EcoICRI | GAGCTC | 1 cut(s) | 739 |
| EcoO65I | GGTNACC | 2 cut(s) | 2324, 2732 |
| EcoRII | CCWGG | 3 cut(s) | 952, 1742, 1980 |
| EcoRV | GATATC | 1 cut(s) | 2585 |
| EcoT14I | CCWWGG | 4 cut(s) | 1560, 1577, 2320, 2421 |
| EcoT22I | ATGCAT | 2 cut(s) | 1720, 2050 |
| EcoT38I | GRGCYC | 2 cut(s) | 741, 2288 |
| EgeI | GGCGCC | 1 cut(s) | 156 |
| EheI | GGCGCC | 1 cut(s) | 156 |
| ErhI | CCWWGG | 4 cut(s) | 1560, 1577, 2320, 2421 |
| Esp3I | CGTCTC | 2 cut(s) | 92, 187 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 599, 631, 1214, 1246 |
| FaqI | GGGAC | 6 cut(s) | 533, 608, 1214, 1267, 2785, 2805 |
| FauI | CCCGC | 7 cut(s) | 58, 301, 383, 606, 633, 856, 912 |
| FauNDI | CATATG | 2 cut(s) | 1618, 2044 |
| FokI | GGATG | 6 cut(s) | 33, 52, 343, 1964, 2277, 2547 |
| FriOI | GRGCYC | 2 cut(s) | 741, 2288 |
| FspBI | CTAG | 1 cut(s) | 1326 |
| GlaI | GCGC | 7 cut(s) | 156, 410, 633, 685, 945, 1044, 1124 |
| GsaI | CCCAGC | 2 cut(s) | 235, 270 |
| GsuI | CTGGAG | 2 cut(s) | 975, 2694 |
| HaeII | RGCGCY | 3 cut(s) | 158, 412, 687 |
| HaeIII | GGCC | 9 cut(s) | 308, 1114, 1609, 1905, 1919, 2053, 2170, 2603, 2781 |
| HgaI | GACGC | 4 cut(s) | 222, 950, 1264, 2355 |
| HhaI | GCGC | 7 cut(s) | 157, 411, 634, 686, 946, 1045, 1125 |
| Hin1I | GRCGYC | 3 cut(s) | 155, 214, 675 |
| Hin6I | GCGC | 7 cut(s) | 155, 409, 632, 684, 944, 1043, 1123 |
| HinP1I | GCGC | 7 cut(s) | 155, 409, 632, 684, 944, 1043, 1123 |
| HincII | GTYRAC | 2 cut(s) | 28, 1314 |
| HindII | GTYRAC | 2 cut(s) | 28, 1314 |
| HindIII | AAGCTT | 1 cut(s) | 1334 |
| HpaI | GTTAAC | 1 cut(s) | 28 |
| HphI | GGTGA | 7 cut(s) | 418, 511, 529, 1366, 1538, 2189, 2726 |
| Hpy166II | GTNNAC | 9 cut(s) | 28, 287, 822, 847, 994, 1057, 1314, 1402, 2718 |
| Hpy8I | GTNNAC | 9 cut(s) | 28, 287, 822, 847, 994, 1057, 1314, 1402, 2718 |
| Hpy99I | CGWCG | 3 cut(s) | 216, 566, 677 |
| HpyAV | CCTTC | 8 cut(s) | 152, 601, 907, 982, 1663, 1687, 1815, 2168 |
| Hsp92I | GRCGYC | 3 cut(s) | 155, 214, 675 |
| HspAI | GCGC | 7 cut(s) | 155, 409, 632, 684, 944, 1043, 1123 |
| KasI | GGCGCC | 1 cut(s) | 154 |
| Kpn2I | TCCGGA | 2 cut(s) | 871, 909 |
| KroI | GCCGGC | 1 cut(s) | 1110 |
| KroNI | GCCGGC | 1 cut(s) | 1112 |
| KspAI | GTTAAC | 1 cut(s) | 28 |
| LguI | GCTCTTC | 1 cut(s) | 2631 |
| LmnI | GCTCC | 8 cut(s) | 325, 610, 874, 912, 926, 1102, 1570, 2072 |
| Lsp1109I | GCAGC | 6 cut(s) | 407, 948, 1104, 1600, 1897, 2533 |
| LweI | GCATC | 9 cut(s) | 502, 1054, 1128, 1705, 1746, 1933, 2353, 2605, 2617 |
| MaeI | CTAG | 1 cut(s) | 1326 |
| MaeIII | GTNAC | 6 cut(s) | 535, 541, 890, 2324, 2592, 2732 |
| MbiI | CCGCTC | 4 cut(s) | 53, 328, 907, 2566 |
| MflI | RGATCY | 6 cut(s) | 708, 868, 1831, 1991, 2459, 2831 |
| MhlI | GDGCHC | 5 cut(s) | 615, 741, 879, 1491, 2288 |
| MlsI | TGGCCA | 1 cut(s) | 2053 |
| MluNI | TGGCCA | 1 cut(s) | 2053 |
| Mly113I | GGCGCC | 1 cut(s) | 155 |
| MlyI | GAGTC | 4 cut(s) | 326, 521, 788, 1865 |
| MmeI | TCCRAC | 5 cut(s) | 300, 957, 1146, 1342, 1628 |
| Mox20I | TGGCCA | 1 cut(s) | 2053 |
| Mph1103I | ATGCAT | 2 cut(s) | 1720, 2050 |
| MroI | TCCGGA | 2 cut(s) | 871, 909 |
| MroNI | GCCGGC | 1 cut(s) | 1110 |
| MroXI | GAANNNNTTC | 3 cut(s) | 1280, 2337, 2699 |
| MscI | TGGCCA | 1 cut(s) | 2053 |
| MseI | TTAA | 7 cut(s) | 8, 27, 125, 645, 1521, 2105, 2124 |
| MslI | CAYNNNNRTG | 6 cut(s) | 395, 1707, 1713, 2047, 2613, 2760 |
| Msp20I | TGGCCA | 1 cut(s) | 2053 |
| MspA1I | CMGCKG | 3 cut(s) | 420, 628, 1117 |
| MspR9I | CCNGG | 9 cut(s) | 568, 713, 748, 775, 954, 1273, 1744, 1982, 2330 |
| Mva1269I | GAATGC | 2 cut(s) | 1288, 1962 |
| MvaI | CCWGG | 3 cut(s) | 954, 1744, 1982 |
| MvnI | CGCG | 4 cut(s) | 944, 1123, 1125, 1663 |
| NaeI | GCCGGC | 1 cut(s) | 1112 |
| NarI | GGCGCC | 1 cut(s) | 155 |
| NciI | CCSGG | 6 cut(s) | 568, 713, 748, 775, 1273, 2330 |
| NcoI | CCATGG | 1 cut(s) | 1577 |
| NdeI | CATATG | 2 cut(s) | 1618, 2044 |
| NgoMIV | GCCGGC | 1 cut(s) | 1110 |
| NheI | GCTAGC | 1 cut(s) | 1325 |
| NmeAIII | GCCGAG | 4 cut(s) | 776, 1122, 1131, 2629 |
| NmuCI | GTSAC | 3 cut(s) | 535, 2592, 2732 |
| NsiI | ATGCAT | 2 cut(s) | 1720, 2050 |
| NspI | RCATGY | 2 cut(s) | 400, 1850 |
| NspV | TTCGAA | 2 cut(s) | 369, 2634 |
| PaeR7I | CTCGAG | 1 cut(s) | 650 |
| PagI | TCATGA | 1 cut(s) | 579 |
| PciI | ACATGT | 1 cut(s) | 396 |
| PciSI | GCTCTTC | 1 cut(s) | 2631 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 208, 306, 351, 657 |
| PctI | GAATGC | 2 cut(s) | 1288, 1962 |
| PdiI | GCCGGC | 1 cut(s) | 1112 |
| PdmI | GAANNNNTTC | 3 cut(s) | 1280, 2337, 2699 |
| PfeI | GAWTC | 8 cut(s) | 723, 970, 1096, 1942, 2139, 2278, 2381, 2700 |
| PflFI | GACNNNGTC | 1 cut(s) | 674 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 304 |
| PfoI | TCCNGGA | 2 cut(s) | 773, 1742 |
| PleI | GAGTC | 4 cut(s) | 325, 521, 788, 1865 |
| PluTI | GGCGCC | 1 cut(s) | 158 |
| PmaCI | CACGTG | 1 cut(s) | 575 |
| PmlI | CACGTG | 1 cut(s) | 575 |
| PpsI | GAGTC | 4 cut(s) | 325, 521, 788, 1865 |
| Ppu21I | YACGTR | 4 cut(s) | 575, 897, 2449, 2758 |
| PscI | ACATGT | 1 cut(s) | 396 |
| PshAI | GACNNNNGTC | 2 cut(s) | 560, 575 |
| PsiI | TTATAA | 3 cut(s) | 1346, 1631, 2061 |
| Psp124BI | GAGCTC | 1 cut(s) | 741 |
| Psp1406I | AACGTT | 1 cut(s) | 1645 |
| Psp6I | CCWGG | 3 cut(s) | 952, 1742, 1980 |
| PspCI | CACGTG | 1 cut(s) | 575 |
| PspEI | GGTNACC | 2 cut(s) | 2324, 2732 |
| PspFI | CCCAGC | 2 cut(s) | 231, 266 |
| PspGI | CCWGG | 3 cut(s) | 952, 1742, 1980 |
| PspPI | GGNCC | 6 cut(s) | 547, 767, 1607, 1863, 2601, 2780 |
| PspXI | VCTCGAGB | 1 cut(s) | 650 |
| PstI | CTGCAG | 2 cut(s) | 2730, 2790 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2075 |
| PsuI | RGATCY | 6 cut(s) | 708, 868, 1831, 1991, 2459, 2831 |
| PsyI | GACNNNGTC | 1 cut(s) | 674 |
| RseI | CAYNNNNRTG | 6 cut(s) | 395, 1707, 1713, 2047, 2613, 2760 |
| SacI | GAGCTC | 1 cut(s) | 741 |
| SapI | GCTCTTC | 1 cut(s) | 2631 |
| SaqAI | TTAA | 7 cut(s) | 8, 27, 125, 645, 1521, 2105, 2124 |
| Sau96I | GGNCC | 6 cut(s) | 547, 767, 1607, 1863, 2601, 2780 |
| SbfI | CCTGCAGG | 1 cut(s) | 2790 |
| ScaI | AGTACT | 1 cut(s) | 2248 |
| SchI | GAGTC | 4 cut(s) | 326, 521, 788, 1865 |
| ScrFI | CCNGG | 9 cut(s) | 568, 713, 748, 775, 954, 1273, 1744, 1982, 2330 |
| SdaI | CCTGCAGG | 1 cut(s) | 2790 |
| SduI | GDGCHC | 5 cut(s) | 615, 741, 879, 1491, 2288 |
| SfaNI | GCATC | 9 cut(s) | 502, 1054, 1128, 1705, 1746, 1933, 2353, 2605, 2617 |
| SfcI | CTRYAG | 3 cut(s) | 1594, 2726, 2786 |
| SfoI | GGCGCC | 1 cut(s) | 156 |
| Sfr274I | CTCGAG | 1 cut(s) | 650 |
| SfuI | TTCGAA | 2 cut(s) | 369, 2634 |
| SinI | GGWCC | 3 cut(s) | 547, 767, 1863 |
| SlaI | CTCGAG | 1 cut(s) | 650 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 395, 1707, 1713, 2047, 2613, 2760 |
| SmlI | CTYRAG | 3 cut(s) | 650, 1154, 2295 |
| SmoI | CTYRAG | 3 cut(s) | 650, 1154, 2295 |
| SnaBI | TACGTA | 1 cut(s) | 2449 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 2790 |
| SspDI | GGCGCC | 1 cut(s) | 154 |
| SspI | AATATT | 1 cut(s) | 2454 |
| SspMI | CTAG | 1 cut(s) | 1326 |
| SstI | GAGCTC | 1 cut(s) | 741 |
| StyD4I | CCNGG | 9 cut(s) | 566, 711, 746, 773, 952, 1271, 1742, 1980, 2328 |
| StyI | CCWWGG | 4 cut(s) | 1560, 1577, 2320, 2421 |
| TaqI | TCGA | 8 cut(s) | 354, 369, 651, 810, 831, 2016, 2587, 2634 |
| TaqII | GACCGA | 1 cut(s) | 2558 |
| TatI | WGTACW | 6 cut(s) | 284, 846, 993, 1158, 1781, 2246 |
| TauI | GCSGC | 7 cut(s) | 381, 420, 1093, 1117, 1123, 1128, 1131 |
| TfiI | GAWTC | 8 cut(s) | 723, 970, 1096, 1942, 2139, 2278, 2381, 2700 |
| Tru1I | TTAA | 7 cut(s) | 8, 27, 125, 645, 1521, 2105, 2124 |
| Tru9I | TTAA | 7 cut(s) | 8, 27, 125, 645, 1521, 2105, 2124 |
| TscAI | CASTG | 9 cut(s) | 294, 608, 849, 953, 1519, 1598, 1887, 2268, 2509 |
| TseFI | GTSAC | 3 cut(s) | 535, 2592, 2732 |
| TseI | GCWGC | 6 cut(s) | 420, 961, 1117, 1613, 1910, 2546 |
| Tsp45I | GTSAC | 3 cut(s) | 535, 2592, 2732 |
| TspGWI | ACGGA | 7 cut(s) | 45, 74, 329, 780, 913, 1052, 2830 |
| TspRI | CASTG | 9 cut(s) | 294, 608, 849, 953, 1519, 1598, 1887, 2268, 2509 |
| Tth111I | GACNNNGTC | 1 cut(s) | 674 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 304 |
| VpaK11BI | GGWCC | 3 cut(s) | 547, 767, 1863 |
| XapI | RAATTY | 2 cut(s) | 2205, 2847 |
| XceI | RCATGY | 2 cut(s) | 400, 1850 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1575 |
| XhoI | CTCGAG | 1 cut(s) | 650 |
| XmnI | GAANNNNTTC | 3 cut(s) | 1280, 2337, 2699 |
| XspI | CTAG | 1 cut(s) | 1326 |
| ZraI | GACGTC | 1 cut(s) | 676 |
| ZrmI | AGTACT | 1 cut(s) | 2248 |
| Zsp2I | ATGCAT | 2 cut(s) | 1720, 2050 |