MD02G1233700.v1.1

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001605
Physical Location & Seq
Reverse (-)
27992796 .. 27994386
1591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1233700.v1.1.491

Sequence Viewer

Length: 1533 bp
ATGTCCCAAAAAGATGAAAATGTAGTTTCCATGAAGAAGGAGCTGTCCATGACGAAAGTTGCGAAAAGAGGAGGTGGACAAGCAAGCGGAGGAGACCAAGGCCAACCTGCAGAAATCACCACCACGGAGCAAACTTCAGAGGAGGCGATTACAATCCATGAGGAGGTAATAAGAACCAGTAGGGGGGAGAAACTAAAGAAAATGAGGGATGCATTGGCAGAAGCAGCTCAAAAGCAAAATGCCGAACCAAAGATCCAAAGGGTTCCCTTCATGCTCCGTGATCACAAAAATTTAGACAAGTACTACGAGCCAAGGGTGGTAGCACTCGGTCCTCGCCACCATGGTAAAGAAAAGTACAAGCAGGCAGAGAAAGTCAAGCTTCAATTGACAGAACACTTTATCAAAGATAGTGAGCAGACTAATAACGATGAAACGTTGCTCAAGAACGTTGAGGACAAGATTAAGGAGCTAAGGGAGTGCTATGATGACGAAGCAACAAAGGACCACGATGATGAGGCCCTCGCGTGGATGCTGTTCGTTGATGGGTGTTCGACGTTGGAATTCATGTACAAATATAAGGAGTTGGAAGCTTTTAAGATCAAGAGAGACCAAGTGGCATTTGTAGAACAGGACATGTTCCTGCTAGAAAACCAACTCCCCTATCAACTCCTCGAGTTGCTGATCAGCTCAAGCAACAAACGTGTGGACTTGGAAGCCTCAATCGAGGAATTTGTCAAGATGCATGGTGGAGCACCAGTAGTTCAAGGCCCTGCCGTGGAAAGAAAGCCAACTCATCTTCTTGACCTTCTTCGGACAAGAATGGTAGGACGTCCACCTAGCCTGAGTACAGAACCAGGATGTACTCATCATATTCTTGACCTTCGGCCAATGGTAGGACCTCGACCTAGCCAGAGTACAAAACCAGGATGTACTCATCGTCTTCTTGACCTTCTTCAGACAATGGCAGCCGGTATCCATTTTAGGCCTAGTGAGAAAAACTCATTGATGGACATATATTTTAAGCCTCTGTTTCTATGCTGTGGATTCCTTTATCTTCCCATAATAAAAGTTGACGACTCGATGGGGCCTAAGTTCATGAACATCATAGCCTACGAAATGTGTCCTGATTTCCAGAACGATTTTGTGGTCACCTCTTACATAGGCTTCCTCGATTCGCTCATTGATCATGCAGACGATGTGAAGCACTTGAGGAAAAAGGGCATAGTTCAAAACTTGCTTGGGAGTGATCAGGAGGTGGCTGAACTCTTCAACGAGATAAGCACTGACTTGGTCCCTAACGATGCGATTTACCGAAATGTTACAGCACAGATAGAATATCACTACAAAACCAAGTGGAAGACATGGATGGCTCAATTCTTTCATGACCATTTCAATAGTCCCTGGACTTTCTTGGGTTTCATTGGCGTTCTACTAGGACTTGGCTTGAGCGGTATGCAGACGTGGTATGCAGCCTTTTCCGATCCCTGTCCATGTGATGCCGTATGCAAGTACCTGAAAACACACCCCGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

511

Amino Acids

58.21

Weight (kDa)

5.94

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 85 - 480 5.1e-92 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 832
Acc36I ACCTGC 1 cut(s) 115
AccBSI CCGCTC 1 cut(s) 1449
AccII CGCG 1 cut(s) 524
AciI CCGC 2 cut(s) 87, 1449
AclI AACGTT 2 cut(s) 434, 447
AclWI GGATC 2 cut(s) 247, 1475
AcoI YGGCCR 1 cut(s) 884
AcsI RAATTY 3 cut(s) 289, 560, 728
AcuI CTGAAG 2 cut(s) 120, 938
AcyI GRCGYC 1 cut(s) 829
AfaI GTAC 8 cut(s) 302, 356, 569, 847, 862, 916, 931, 1511
AfiI CCNNNNNNNGG 5 cut(s) 163, 183, 525, 775, 893
AflIII ACRYGT 2 cut(s) 633, 700
AgsI TTSAA 5 cut(s) 383, 764, 1229, 1270, 1393
AjiI CACGTC 1 cut(s) 1461
AjnI CCWGG 3 cut(s) 853, 922, 1400
AloI GAACNNNNNNTCC 2 cut(s) 237, 269
AluBI AGCT 6 cut(s) 43, 227, 379, 469, 590, 687
AluI AGCT 6 cut(s) 43, 227, 379, 469, 590, 687
Alw21I GWGCWC 1 cut(s) 754
Alw26I GTCTC 2 cut(s) 87, 600
AlwI GGATC 2 cut(s) 247, 1475
Ama87I CYCGRG 1 cut(s) 671
AoxI GGCC 6 cut(s) 100, 516, 766, 884, 983, 1085
ApeKI GCWGC 3 cut(s) 224, 965, 1469
ApoI RAATTY 3 cut(s) 289, 560, 728
AspS9I GGNCC 7 cut(s) 329, 502, 517, 767, 896, 1085, 1291
AsuC2I CCSGG 1 cut(s) 1527
AsuHPI GGTGA 2 cut(s) 109, 1141
AvaI CYCGRG 1 cut(s) 671
AvaII GGWCC 4 cut(s) 329, 502, 896, 1291
BbsI GAAGAC 2 cut(s) 932, 1364
Bbv12I GWGCWC 1 cut(s) 754
BbvI GCAGC 3 cut(s) 236, 977, 1481
BccI CCATC 4 cut(s) 536, 1000, 1075, 1360
BceAI ACGGC 2 cut(s) 758, 1484
BciT130I CCWGG 3 cut(s) 855, 924, 1402
BciVI GTATCC 1 cut(s) 983
BclI TGATCA 4 cut(s) 280, 681, 1183, 1246
BcnI CCSGG 1 cut(s) 1527
BcoDI GTCTC 2 cut(s) 87, 600
BfaI CTAG 5 cut(s) 644, 837, 906, 987, 1433
BfmI CTRYAG 1 cut(s) 108
BfuAI ACCTGC 1 cut(s) 115
BfuI GTATCC 1 cut(s) 983
BisI GCNGC 3 cut(s) 225, 966, 1470
BlsI GCNGC 3 cut(s) 226, 967, 1471
BmcAI AGTACT 1 cut(s) 302
Bme1390I CCNGG 4 cut(s) 855, 924, 1402, 1527
Bme18I GGWCC 4 cut(s) 329, 502, 896, 1291
BmeT110I CYCGRG 1 cut(s) 671
BmgBI CACGTC 1 cut(s) 1461
BmgT120I GGNCC 7 cut(s) 329, 502, 517, 767, 896, 1085, 1291
BmiI GGNNCC 3 cut(s) 264, 1086, 1293
BmrFI CCNGG 4 cut(s) 855, 924, 1402, 1527
BmsI GCATC 5 cut(s) 199, 519, 729, 1291, 1486
BpiI GAAGAC 2 cut(s) 932, 1364
BplI GAGNNNNNCTC 2 cut(s) 983, 1015
Bpu10I CCTNAGC 1 cut(s) 470
BpuEI CTTGAG 4 cut(s) 425, 673, 1228, 1465
BpuMI CCSGG 1 cut(s) 1527
BsaBI GATNNNNATC 1 cut(s) 152
BsaHI GRCGYC 1 cut(s) 829
BsaI GGTCTC 2 cut(s) 87, 600
BsaJI CCNNGG 7 cut(s) 97, 123, 311, 340, 774, 1400, 1525
BsaXI ACNNNNNCTCC 2 cut(s) 741, 771
Bsc4I CCNNNNNNNGG 5 cut(s) 163, 183, 525, 775, 893
Bse118I RCCGGY 1 cut(s) 968
Bse1I ACTGG 2 cut(s) 177, 755
Bse8I GATNNNNATC 1 cut(s) 152
BseBI CCWGG 3 cut(s) 855, 924, 1402
BseDI CCNNGG 7 cut(s) 97, 123, 311, 340, 774, 1400, 1525
BseGI GGATG 5 cut(s) 214, 534, 863, 932, 1371
BseJI GATNNNNATC 1 cut(s) 152
BseLI CCNNNNNNNGG 5 cut(s) 163, 183, 525, 775, 893
BseMII CTCAG 1 cut(s) 833
BseNI ACTGG 2 cut(s) 177, 755
BseRI GAGGAG 5 cut(s) 84, 105, 155, 176, 659
BseXI GCAGC 3 cut(s) 236, 977, 1481
Bsh1236I CGCG 1 cut(s) 524
BshFI GGCC 6 cut(s) 102, 518, 768, 886, 985, 1087
BsiHKAI GWGCWC 1 cut(s) 754
BsiHKCI CYCGRG 1 cut(s) 671
BsiSI CCGG 2 cut(s) 969, 1527
BslFI GGGAC 2 cut(s) 1277, 1383
BslI CCNNNNNNNGG 5 cut(s) 163, 183, 525, 775, 893
BsmAI GTCTC 2 cut(s) 87, 600
BsmFI GGGAC 2 cut(s) 1277, 1383
BsnI GGCC 6 cut(s) 102, 518, 768, 886, 985, 1087
Bso31I GGTCTC 2 cut(s) 87, 600
BsoBI CYCGRG 1 cut(s) 671
Bsp1286I GDGCHC 1 cut(s) 754
Bsp1407I TGTACA 1 cut(s) 567
Bsp143I GATC 7 cut(s) 252, 280, 597, 681, 1183, 1246, 1480
Bsp19I CCATGG 1 cut(s) 340
BspACI CCGC 2 cut(s) 87, 1449
BspANI GGCC 6 cut(s) 102, 518, 768, 886, 985, 1087
BspCNI CTCAG 1 cut(s) 834
BspFNI CGCG 1 cut(s) 524
BspHI TCATGA 2 cut(s) 1095, 1381
BspLI GGNNCC 3 cut(s) 264, 1086, 1293
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 1 cut(s) 115
BspPI GGATC 2 cut(s) 247, 1475
BspTNI GGTCTC 2 cut(s) 87, 600
BsrBI CCGCTC 1 cut(s) 1449
BsrFI RCCGGY 1 cut(s) 968
BsrGI TGTACA 1 cut(s) 567
BsrI ACTGG 2 cut(s) 177, 755
BssAI RCCGGY 1 cut(s) 968
BssECI CCNNGG 7 cut(s) 97, 123, 311, 340, 774, 1400, 1525
BssMI GATC 7 cut(s) 252, 280, 597, 681, 1183, 1246, 1480
BssNI GRCGYC 1 cut(s) 829
BssT1I CCWWGG 3 cut(s) 97, 311, 340
Bst2UI CCWGG 3 cut(s) 855, 924, 1402
Bst6I CTCTTC 1 cut(s) 1271
BstACI GRCGYC 1 cut(s) 829
BstAUI TGTACA 1 cut(s) 567
BstC8I GCNNGC 2 cut(s) 85, 363
BstDEI CTNAG 3 cut(s) 470, 842, 1089
BstDSI CCRYGG 3 cut(s) 123, 340, 774
BstEII GGTNACC 1 cut(s) 1147
BstF5I GGATG 5 cut(s) 214, 534, 863, 932, 1371
BstFNI CGCG 1 cut(s) 524
BstKTI GATC 7 cut(s) 255, 283, 600, 684, 1186, 1249, 1483
BstMAI GTCTC 2 cut(s) 87, 600
BstMBI GATC 7 cut(s) 252, 280, 597, 681, 1183, 1246, 1480
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNI CCWGG 3 cut(s) 855, 924, 1402
BstNSI RCATGY 1 cut(s) 637
BstPI GGTNACC 1 cut(s) 1147
BstSCI CCNGG 4 cut(s) 853, 922, 1400, 1525
BstSFI CTRYAG 1 cut(s) 108
BstUI CGCG 1 cut(s) 524
BstV1I GCAGC 3 cut(s) 236, 977, 1481
BstV2I GAAGAC 2 cut(s) 932, 1364
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BsuI GTATCC 1 cut(s) 983
BsuRI GGCC 6 cut(s) 102, 518, 768, 886, 985, 1087
BtgI CCRYGG 3 cut(s) 123, 340, 774
BtrI CACGTC 1 cut(s) 1461
BtsCI GGATG 5 cut(s) 214, 534, 863, 932, 1371
BtsIMutI CAGTG 1 cut(s) 1281
BveI ACCTGC 1 cut(s) 115
Cac8I GCNNGC 2 cut(s) 85, 363
CciI TCATGA 2 cut(s) 1095, 1381
Cfr10I RCCGGY 1 cut(s) 968
Cfr13I GGNCC 7 cut(s) 329, 502, 517, 767, 896, 1085, 1291
Csp6I GTAC 8 cut(s) 301, 355, 568, 846, 861, 915, 930, 1510
CviQI GTAC 8 cut(s) 301, 355, 568, 846, 861, 915, 930, 1510
DdeI CTNAG 3 cut(s) 470, 842, 1089
DpnI GATC 7 cut(s) 254, 282, 599, 683, 1185, 1248, 1482
DpnII GATC 7 cut(s) 252, 280, 597, 681, 1183, 1246, 1480
EaeI YGGCCR 1 cut(s) 884
Eam1104I CTCTTC 1 cut(s) 1271
EarI CTCTTC 1 cut(s) 1271
Eco130I CCWWGG 3 cut(s) 97, 311, 340
Eco147I AGGCCT 1 cut(s) 985
Eco31I GGTCTC 2 cut(s) 87, 600
Eco47I GGWCC 4 cut(s) 329, 502, 896, 1291
Eco57I CTGAAG 2 cut(s) 120, 938
Eco88I CYCGRG 1 cut(s) 671
Eco91I GGTNACC 1 cut(s) 1147
EcoO109I RGGNCCY 4 cut(s) 517, 767, 896, 1085
EcoO65I GGTNACC 1 cut(s) 1147
EcoRI GAATTC 1 cut(s) 560
EcoRII CCWGG 3 cut(s) 853, 922, 1400
EcoT14I CCWWGG 3 cut(s) 97, 311, 340
EcoT22I ATGCAT 2 cut(s) 214, 744
ErhI CCWWGG 3 cut(s) 97, 311, 340
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FaqI GGGAC 2 cut(s) 1277, 1383
FbaI TGATCA 4 cut(s) 280, 681, 1183, 1246
Fnu4HI GCNGC 3 cut(s) 225, 966, 1470
FokI GGATG 5 cut(s) 221, 541, 870, 939, 1378
Fsp4HI GCNGC 3 cut(s) 225, 966, 1470
FspBI CTAG 5 cut(s) 644, 837, 906, 987, 1433
GluI GCNGC 3 cut(s) 225, 966, 1470
HaeIII GGCC 6 cut(s) 102, 518, 768, 886, 985, 1087
HapII CCGG 2 cut(s) 969, 1527
Hin1I GRCGYC 1 cut(s) 829
HincII GTYRAC 1 cut(s) 1072
HindII GTYRAC 1 cut(s) 1072
HindIII AAGCTT 2 cut(s) 377, 588
HinfI GANTC 3 cut(s) 1044, 1076, 1172
HpaII CCGG 2 cut(s) 969, 1527
HphI GGTGA 2 cut(s) 109, 1141
Hpy166II GTNNAC 4 cut(s) 77, 706, 833, 1072
Hpy188I TCNGA 4 cut(s) 139, 813, 957, 1480
Hpy8I GTNNAC 4 cut(s) 77, 706, 833, 1072
Hpy99I CGWCG 1 cut(s) 556
HpyAV CCTTC 5 cut(s) 31, 277, 815, 890, 959
HpyCH4IV ACGT 6 cut(s) 434, 447, 554, 700, 829, 1460
HpyCH4V TGCA 7 cut(s) 110, 212, 742, 1190, 1456, 1469, 1506
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 3 cut(s) 470, 842, 1089
HpySE526I ACGT 6 cut(s) 434, 447, 554, 700, 829, 1460
Hsp92I GRCGYC 1 cut(s) 829
Ksp22I TGATCA 4 cut(s) 280, 681, 1183, 1246
Kzo9I GATC 7 cut(s) 252, 280, 597, 681, 1183, 1246, 1480
LmnI GCTCC 5 cut(s) 40, 127, 279, 466, 749
Lsp1109I GCAGC 3 cut(s) 236, 977, 1481
LweI GCATC 5 cut(s) 199, 519, 729, 1291, 1486
MaeI CTAG 5 cut(s) 644, 837, 906, 987, 1433
MaeII ACGT 6 cut(s) 434, 447, 554, 700, 829, 1460
MaeIII GTNAC 2 cut(s) 1147, 1318
MalI GATC 7 cut(s) 254, 282, 599, 683, 1185, 1248, 1482
MbiI CCGCTC 1 cut(s) 1449
MboI GATC 7 cut(s) 252, 280, 597, 681, 1183, 1246, 1480
MboII GAAGA 8 cut(s) 46, 788, 800, 932, 944, 1046, 1258, 1369
MfeI CAATTG 1 cut(s) 383
MflI RGATCY 1 cut(s) 252
MhlI GDGCHC 1 cut(s) 754
MluCI AATT 5 cut(s) 289, 383, 560, 728, 1373
MlyI GAGTC 1 cut(s) 1070
MmeI TCCRAC 2 cut(s) 537, 564
Mph1103I ATGCAT 2 cut(s) 214, 744
MseI TTAA 3 cut(s) 462, 594, 1020
MslI CAYNNNNRTG 1 cut(s) 510
MspI CCGG 2 cut(s) 969, 1527
MspR9I CCNGG 4 cut(s) 855, 924, 1402, 1527
MunI CAATTG 1 cut(s) 383
MvaI CCWGG 3 cut(s) 855, 924, 1402
MvnI CGCG 1 cut(s) 524
MwoI GCNNNNNNNGC 1 cut(s) 224
NciI CCSGG 1 cut(s) 1527
NcoI CCATGG 1 cut(s) 340
NdeII GATC 7 cut(s) 252, 280, 597, 681, 1183, 1246, 1480
NlaIV GGNNCC 3 cut(s) 264, 1086, 1293
NmuCI GTSAC 1 cut(s) 1147
NsiI ATGCAT 2 cut(s) 214, 744
NspI RCATGY 1 cut(s) 637
PaeR7I CTCGAG 1 cut(s) 671
PagI TCATGA 2 cut(s) 1095, 1381
PceI AGGCCT 1 cut(s) 985
PciI ACATGT 1 cut(s) 633
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PfeI GAWTC 2 cut(s) 1044, 1172
PflFI GACNNNGTC 1 cut(s) 1289
PkrI GCNGC 3 cut(s) 226, 967, 1471
PleI GAGTC 1 cut(s) 1070
PpsI GAGTC 1 cut(s) 1070
PpuMI RGGWCCY 1 cut(s) 896
PscI ACATGT 1 cut(s) 633
Psp1406I AACGTT 2 cut(s) 434, 447
Psp5II RGGWCCY 1 cut(s) 896
Psp6I CCWGG 3 cut(s) 853, 922, 1400
PspEI GGTNACC 1 cut(s) 1147
PspGI CCWGG 3 cut(s) 853, 922, 1400
PspN4I GGNNCC 3 cut(s) 264, 1086, 1293
PspPI GGNCC 7 cut(s) 329, 502, 517, 767, 896, 1085, 1291
PspPPI RGGWCCY 1 cut(s) 896
PspXI VCTCGAGB 1 cut(s) 671
PstI CTGCAG 1 cut(s) 112
PsuI RGATCY 1 cut(s) 252
PsyI GACNNNGTC 1 cut(s) 1289
RsaI GTAC 8 cut(s) 302, 356, 569, 847, 862, 916, 931, 1511
RsaNI GTAC 8 cut(s) 301, 355, 568, 846, 861, 915, 930, 1510
RseI CAYNNNNRTG 1 cut(s) 510
SaqAI TTAA 3 cut(s) 462, 594, 1020
SatI GCNGC 3 cut(s) 225, 966, 1470
Sau3AI GATC 7 cut(s) 252, 280, 597, 681, 1183, 1246, 1480
Sau96I GGNCC 7 cut(s) 329, 502, 517, 767, 896, 1085, 1291
ScaI AGTACT 1 cut(s) 302
SchI GAGTC 1 cut(s) 1070
ScrFI CCNGG 4 cut(s) 855, 924, 1402, 1527
SduI GDGCHC 1 cut(s) 754
SfaNI GCATC 5 cut(s) 199, 519, 729, 1291, 1486
SfcI CTRYAG 1 cut(s) 108
Sfr274I CTCGAG 1 cut(s) 671
SinI GGWCC 4 cut(s) 329, 502, 896, 1291
SlaI CTCGAG 1 cut(s) 671
SmiMI CAYNNNNRTG 1 cut(s) 510
SmlI CTYRAG 5 cut(s) 440, 671, 688, 1207, 1444
SmoI CTYRAG 5 cut(s) 440, 671, 688, 1207, 1444
Sse9I AATT 5 cut(s) 289, 383, 560, 728, 1373
SseBI AGGCCT 1 cut(s) 985
SsiI CCGC 2 cut(s) 87, 1449
SspMI CTAG 5 cut(s) 644, 837, 906, 987, 1433
StuI AGGCCT 1 cut(s) 985
StyD4I CCNGG 4 cut(s) 853, 922, 1400, 1525
StyI CCWWGG 3 cut(s) 97, 311, 340
TaiI ACGT 6 cut(s) 437, 450, 557, 703, 832, 1463
TaqI TCGA 6 cut(s) 551, 672, 723, 901, 1079, 1170
TaqII GACCGA 1 cut(s) 317
TasI AATT 5 cut(s) 289, 383, 560, 728, 1373
TatI WGTACW 7 cut(s) 300, 354, 567, 845, 860, 914, 929
TfiI GAWTC 2 cut(s) 1044, 1172
Tru1I TTAA 3 cut(s) 462, 594, 1020
Tru9I TTAA 3 cut(s) 462, 594, 1020
TscAI CASTG 1 cut(s) 1288
TseFI GTSAC 1 cut(s) 1147
TseI GCWGC 3 cut(s) 224, 965, 1469
Tsp45I GTSAC 1 cut(s) 1147
TspDTI ATGAA 9 cut(s) 30, 47, 259, 444, 553, 1084, 1112, 1370, 1408
TspGWI ACGGA 2 cut(s) 140, 266
TspRI CASTG 1 cut(s) 1288
Tth111I GACNNNGTC 1 cut(s) 1289
VpaK11BI GGWCC 4 cut(s) 329, 502, 896, 1291
XapI RAATTY 3 cut(s) 289, 560, 728
XceI RCATGY 1 cut(s) 637
XhoI CTCGAG 1 cut(s) 671
XspI CTAG 5 cut(s) 644, 837, 906, 987, 1433
ZraI GACGTC 1 cut(s) 830
ZrmI AGTACT 1 cut(s) 302
Zsp2I ATGCAT 2 cut(s) 214, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.