pycom12g02050

Plant protein of unknown function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001605
Physical Location & Seq
Reverse (-)
1751391 .. 1752497
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02050.1

Sequence Viewer

Length: 960 bp
ATGGCTGATTCCTTGGAGCAGATGTTTAATAGATTGAAGGATGCAGGAACAAAAAATCAGGATAGTTCTACAAGTGTTGTTCCAAGCAGGTCACGACCGATCATCCAAAGTGTTTCCAGAACTCTCCGAAGTAATAAAAATTTGGAGAAATACTTTGAGCCAAGGGTACTAGCAATAGGCCCTATCCATCATGGAAAGTTTCCGGAATGGGAGGAGCTCAAATACAGATTGGCAGCGGACTTTGTCAACGACAGCGGCCGGAGCGAAGAATTTTTGTACAAGGAGATTGAGGACAACATCAAGGAGTTACAACAATGCTACGACGAGAAAACAACAGAGCGCTATGATGGTGATGAGGCGAGCCTCGCCTGGATGTTTTTCGTAGACGGTTGTTCAATCTTGCAGTTTATACACAAGTATTCTACCCTGGAAGAGTTCGAGATAAAGAGAGACCAAGTGTTCTTTGCTGAACAAGACTTGTTCTTGCTAGAGAACCAAATCCCTTATGAAATTCTCCACCTTTTGATGAGCTCAAGCATAAAAAAAAAGGACCTTTGGAACGGAATCCAGGTGTTTCTCGATCTGCATAGTACTGCAGCTTGTGCATCAGCGAAGCCGTTCAGATGGTATAAAAGGATGATGCGACAAGATCTGAAATTTGAATGGCGTTTGGGAGTATGTGTGGAAGATTCATGGCCTACTCACCTTCTCGAGCTTCTTCGAACAAGAATGCTAGGATTTCTCTTTCTTCCACCAATGAACGCGGACGCAATGCCTTTGTTCTTGAATTTGATTGCTTACGAAATGTGTCCCGATTTCCAAAATGATTACGCGGTCACCTCTTACTTAAGCTTCCTGAGTTCACTCATTACTCATCCCGAAGATGCAAAGCAGCTAAGGTCAGCCCGCATACTTCACAATAAACTGACGAAGCACTCGCTCAACTCTTCAACGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

320

Amino Acids

37.38

Weight (kDa)

5.9

Isoelectric Point (pI)

57.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 35 - 244 2.1e-32 Plant protein of unknown function
DUF247 PF03140 248 - 312 1.5e-13 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 78
AccI GTMKAC 1 cut(s) 384
AccII CGCG 2 cut(s) 764, 833
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 5 cut(s) 236, 255, 764, 833, 907
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 5 cut(s) 139, 269, 510, 656, 787
AfaI GTAC 3 cut(s) 168, 278, 592
AfeI AGCGCT 1 cut(s) 341
AflII CTTAAG 1 cut(s) 847
AgsI TTSAA 5 cut(s) 37, 396, 662, 787, 951
AjnI CCWGG 3 cut(s) 368, 426, 567
AluBI AGCT 6 cut(s) 217, 531, 599, 715, 852, 895
AluI AGCT 6 cut(s) 217, 531, 599, 715, 852, 895
Alw21I GWGCWC 2 cut(s) 219, 533
Alw26I GTCTC 1 cut(s) 444
Ama87I CYCGRG 1 cut(s) 710
Aor13HI TCCGGA 1 cut(s) 202
Aor51HI AGCGCT 1 cut(s) 341
AoxI GGCC 3 cut(s) 178, 256, 695
ApeKI GCWGC 3 cut(s) 233, 596, 892
ApoI RAATTY 5 cut(s) 139, 269, 510, 656, 787
Asp700I GAANNNNTTC 1 cut(s) 617
AspLEI GCGC 1 cut(s) 342
AspS9I GGNCC 2 cut(s) 179, 550
AsuHPI GGTGA 3 cut(s) 362, 695, 829
AsuII TTCGAA 1 cut(s) 721
AvaI CYCGRG 1 cut(s) 710
AvaII GGWCC 1 cut(s) 550
BanII GRGCYC 2 cut(s) 219, 533
Bbv12I GWGCWC 2 cut(s) 219, 533
BbvI GCAGC 3 cut(s) 245, 608, 904
BccI CCATC 3 cut(s) 195, 341, 618
BceAI ACGGC 1 cut(s) 601
BciT130I CCWGG 3 cut(s) 370, 428, 569
BcoDI GTCTC 1 cut(s) 444
BfaI CTAG 3 cut(s) 170, 488, 734
BfmI CTRYAG 1 cut(s) 594
BfoI RGCGCY 1 cut(s) 343
BfrI CTTAAG 1 cut(s) 847
BfuAI ACCTGC 1 cut(s) 78
BglII AGATCT 1 cut(s) 649
BisI GCNGC 4 cut(s) 234, 256, 597, 893
BlsI GCNGC 4 cut(s) 235, 257, 598, 894
BmcAI AGTACT 1 cut(s) 592
Bme1390I CCNGG 3 cut(s) 370, 428, 569
Bme18I GGWCC 1 cut(s) 550
BmeT110I CYCGRG 1 cut(s) 710
BmgT120I GGNCC 2 cut(s) 179, 550
BmrFI CCNGG 3 cut(s) 370, 428, 569
BmsI GCATC 4 cut(s) 31, 614, 630, 874
Bpu10I CCTNAGC 1 cut(s) 896
Bpu14I TTCGAA 1 cut(s) 721
BpuEI CTTGAG 1 cut(s) 517
BsaI GGTCTC 1 cut(s) 444
BsaJI CCNNGG 3 cut(s) 12, 161, 426
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 666, 696
Bse3DI GCAATG 1 cut(s) 777
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 3 cut(s) 370, 428, 569
BseDI CCNNGG 3 cut(s) 12, 161, 426
BseGI GGATG 5 cut(s) 46, 102, 378, 642, 874
BseMI GCAATG 1 cut(s) 777
BseMII CTCAG 1 cut(s) 848
BseRI GAGGAG 1 cut(s) 227
BseX3I CGGCCG 1 cut(s) 256
BseXI GCAGC 3 cut(s) 245, 608, 904
Bsh1236I CGCG 2 cut(s) 764, 833
Bsh1285I CGRYCG 2 cut(s) 98, 259
BshFI GGCC 3 cut(s) 180, 258, 697
BsiEI CGRYCG 2 cut(s) 98, 259
BsiHKAI GWGCWC 2 cut(s) 219, 533
BsiHKCI CYCGRG 1 cut(s) 710
BsiSI CCGG 2 cut(s) 203, 259
BslFI GGGAC 1 cut(s) 795
BsmAI GTCTC 1 cut(s) 444
BsmFI GGGAC 1 cut(s) 795
BsmI GAATGC 1 cut(s) 735
BsnI GGCC 3 cut(s) 180, 258, 697
Bso31I GGTCTC 1 cut(s) 444
BsoBI CYCGRG 1 cut(s) 710
Bsp119I TTCGAA 1 cut(s) 721
Bsp1286I GDGCHC 2 cut(s) 219, 533
Bsp13I TCCGGA 1 cut(s) 202
Bsp1407I TGTACA 1 cut(s) 276
Bsp143I GATC 3 cut(s) 99, 580, 649
BspACI CCGC 5 cut(s) 236, 255, 764, 833, 907
BspANI GGCC 3 cut(s) 180, 258, 697
BspCNI CTCAG 1 cut(s) 849
BspEI TCCGGA 1 cut(s) 202
BspFNI CGCG 2 cut(s) 764, 833
BspMAI CTGCAG 1 cut(s) 598
BspMI ACCTGC 1 cut(s) 78
BspT104I TTCGAA 1 cut(s) 721
BspTI CTTAAG 1 cut(s) 847
BspTNI GGTCTC 1 cut(s) 444
BsrDI GCAATG 1 cut(s) 777
BsrGI TGTACA 1 cut(s) 276
BssECI CCNNGG 3 cut(s) 12, 161, 426
BssMI GATC 3 cut(s) 99, 580, 649
BssT1I CCWWGG 2 cut(s) 12, 161
Bst2UI CCWGG 3 cut(s) 370, 428, 569
Bst4CI ACNGT 1 cut(s) 389
Bst6I CTCTTC 2 cut(s) 426, 952
BstAFI CTTAAG 1 cut(s) 847
BstAPI GCANNNNNTGC 1 cut(s) 602
BstAUI TGTACA 1 cut(s) 276
BstBI TTCGAA 1 cut(s) 721
BstC8I GCNNGC 2 cut(s) 361, 907
BstDEI CTNAG 2 cut(s) 857, 896
BstEII GGTNACC 1 cut(s) 835
BstF5I GGATG 5 cut(s) 46, 102, 378, 642, 874
BstFNI CGCG 2 cut(s) 764, 833
BstH2I RGCGCY 1 cut(s) 343
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 3 cut(s) 102, 583, 652
BstMAI GTCTC 1 cut(s) 444
BstMBI GATC 3 cut(s) 99, 580, 649
BstMCI CGRYCG 2 cut(s) 98, 259
BstMWI GCNNNNNNNGC 3 cut(s) 261, 365, 602
BstNI CCWGG 3 cut(s) 370, 428, 569
BstPI GGTNACC 1 cut(s) 835
BstSCI CCNGG 3 cut(s) 368, 426, 567
BstSFI CTRYAG 1 cut(s) 594
BstUI CGCG 2 cut(s) 764, 833
BstV1I GCAGC 3 cut(s) 245, 608, 904
BstX2I RGATCY 1 cut(s) 649
BstYI RGATCY 1 cut(s) 649
BstZI CGGCCG 1 cut(s) 256
BsuRI GGCC 3 cut(s) 180, 258, 697
BtsCI GGATG 5 cut(s) 46, 102, 378, 642, 874
BveI ACCTGC 1 cut(s) 78
Cac8I GCNNGC 2 cut(s) 361, 907
CfoI GCGC 1 cut(s) 342
Cfr13I GGNCC 2 cut(s) 179, 550
CseI GACGC 1 cut(s) 776
Csp6I GTAC 3 cut(s) 167, 277, 591
CviAII CATG 2 cut(s) 191, 693
CviQI GTAC 3 cut(s) 167, 277, 591
DdeI CTNAG 2 cut(s) 857, 896
DpnI GATC 3 cut(s) 101, 582, 651
DpnII GATC 3 cut(s) 99, 580, 649
EaeI YGGCCR 1 cut(s) 256
EagI CGGCCG 1 cut(s) 256
Eam1104I CTCTTC 2 cut(s) 426, 952
EarI CTCTTC 2 cut(s) 426, 952
Ecl136II GAGCTC 2 cut(s) 217, 531
EclXI CGGCCG 1 cut(s) 256
Eco130I CCWWGG 2 cut(s) 12, 161
Eco24I GRGCYC 2 cut(s) 219, 533
Eco31I GGTCTC 1 cut(s) 444
Eco47I GGWCC 1 cut(s) 550
Eco47III AGCGCT 1 cut(s) 341
Eco52I CGGCCG 1 cut(s) 256
Eco53kI GAGCTC 2 cut(s) 217, 531
Eco88I CYCGRG 1 cut(s) 710
Eco91I GGTNACC 1 cut(s) 835
EcoICRI GAGCTC 2 cut(s) 217, 531
EcoO109I RGGNCCY 2 cut(s) 179, 550
EcoO65I GGTNACC 1 cut(s) 835
EcoRII CCWGG 3 cut(s) 368, 426, 567
EcoT14I CCWWGG 2 cut(s) 12, 161
EcoT38I GRGCYC 2 cut(s) 219, 533
ErhI CCWWGG 2 cut(s) 12, 161
FaeI CATG 2 cut(s) 194, 696
FaqI GGGAC 1 cut(s) 795
FatI CATG 2 cut(s) 190, 692
FauI CCCGC 1 cut(s) 914
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 4 cut(s) 234, 256, 597, 893
FokI GGATG 5 cut(s) 53, 89, 385, 649, 861
FriOI GRGCYC 2 cut(s) 219, 533
Fsp4HI GCNGC 4 cut(s) 234, 256, 597, 893
FspBI CTAG 3 cut(s) 170, 488, 734
GlaI GCGC 1 cut(s) 341
GluI GCNGC 4 cut(s) 234, 256, 597, 893
HaeII RGCGCY 1 cut(s) 343
HaeIII GGCC 3 cut(s) 180, 258, 697
HapII CCGG 2 cut(s) 203, 259
HgaI GACGC 1 cut(s) 776
HhaI GCGC 1 cut(s) 342
Hin1II CATG 2 cut(s) 194, 696
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 850
HinfI GANTC 3 cut(s) 8, 564, 689
HpaII CCGG 2 cut(s) 203, 259
HphI GGTGA 3 cut(s) 362, 695, 829
Hpy166II GTNNAC 3 cut(s) 247, 385, 863
Hpy188I TCNGA 3 cut(s) 128, 623, 654
Hpy8I GTNNAC 3 cut(s) 247, 385, 863
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 2 cut(s) 31, 716
HpyCH4III ACNGT 1 cut(s) 389
HpyCH4V TGCA 6 cut(s) 44, 403, 586, 596, 605, 887
HpyF10VI GCNNNNNNNGC 3 cut(s) 261, 365, 602
HpyF3I CTNAG 2 cut(s) 857, 896
Hsp92II CATG 2 cut(s) 194, 696
HspAI GCGC 1 cut(s) 340
Kpn2I TCCGGA 1 cut(s) 202
Kzo9I GATC 3 cut(s) 99, 580, 649
LmnI GCTCC 3 cut(s) 16, 214, 261
Lsp1109I GCAGC 3 cut(s) 245, 608, 904
LweI GCATC 4 cut(s) 31, 614, 630, 874
MaeI CTAG 3 cut(s) 170, 488, 734
MaeIII GTNAC 3 cut(s) 90, 306, 835
MalI GATC 3 cut(s) 101, 582, 651
MboI GATC 3 cut(s) 99, 580, 649
MboII GAAGA 7 cut(s) 278, 443, 698, 710, 740, 893, 939
MflI RGATCY 1 cut(s) 649
MhlI GDGCHC 2 cut(s) 219, 533
MluCI AATT 5 cut(s) 139, 269, 510, 656, 787
MnlI CCTC 5 cut(s) 205, 283, 349, 374, 850
MroI TCCGGA 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 617
MseI TTAA 2 cut(s) 27, 848
MspA1I CMGCKG 2 cut(s) 236, 255
MspCI CTTAAG 1 cut(s) 847
MspI CCGG 2 cut(s) 203, 259
MspR9I CCNGG 3 cut(s) 370, 428, 569
Mva1269I GAATGC 1 cut(s) 735
MvaI CCWGG 3 cut(s) 370, 428, 569
MvnI CGCG 2 cut(s) 764, 833
MwoI GCNNNNNNNGC 3 cut(s) 261, 365, 602
NdeII GATC 3 cut(s) 99, 580, 649
NlaIII CATG 2 cut(s) 194, 696
NmuCI GTSAC 2 cut(s) 90, 835
NspV TTCGAA 1 cut(s) 721
PaeR7I CTCGAG 1 cut(s) 710
PctI GAATGC 1 cut(s) 735
PdmI GAANNNNTTC 1 cut(s) 617
PfeI GAWTC 3 cut(s) 8, 564, 689
PflFI GACNNNGTC 1 cut(s) 242
PkrI GCNGC 4 cut(s) 235, 257, 598, 894
PpuMI RGGWCCY 1 cut(s) 550
Psp124BI GAGCTC 2 cut(s) 219, 533
Psp5II RGGWCCY 1 cut(s) 550
Psp6I CCWGG 3 cut(s) 368, 426, 567
PspEI GGTNACC 1 cut(s) 835
PspGI CCWGG 3 cut(s) 368, 426, 567
PspPI GGNCC 2 cut(s) 179, 550
PspPPI RGGWCCY 1 cut(s) 550
PstI CTGCAG 1 cut(s) 598
PsuI RGATCY 1 cut(s) 649
PsyI GACNNNGTC 1 cut(s) 242
RsaI GTAC 3 cut(s) 168, 278, 592
RsaNI GTAC 3 cut(s) 167, 277, 591
SacI GAGCTC 2 cut(s) 219, 533
SaqAI TTAA 2 cut(s) 27, 848
SatI GCNGC 4 cut(s) 234, 256, 597, 893
Sau3AI GATC 3 cut(s) 99, 580, 649
Sau96I GGNCC 2 cut(s) 179, 550
ScaI AGTACT 1 cut(s) 592
ScrFI CCNGG 3 cut(s) 370, 428, 569
SduI GDGCHC 2 cut(s) 219, 533
SfaNI GCATC 4 cut(s) 31, 614, 630, 874
SfcI CTRYAG 1 cut(s) 594
Sfr274I CTCGAG 1 cut(s) 710
SfuI TTCGAA 1 cut(s) 721
SinI GGWCC 1 cut(s) 550
SlaI CTCGAG 1 cut(s) 710
SmlI CTYRAG 3 cut(s) 532, 710, 847
SmoI CTYRAG 3 cut(s) 532, 710, 847
Sse9I AATT 5 cut(s) 139, 269, 510, 656, 787
SsiI CCGC 5 cut(s) 236, 255, 764, 833, 907
SspMI CTAG 3 cut(s) 170, 488, 734
SstI GAGCTC 2 cut(s) 219, 533
StyD4I CCNGG 3 cut(s) 368, 426, 567
StyI CCWWGG 2 cut(s) 12, 161
TaaI ACNGT 1 cut(s) 389
TaqI TCGA 4 cut(s) 438, 579, 711, 721
TaqII GACCGA 1 cut(s) 112
TasI AATT 5 cut(s) 139, 269, 510, 656, 787
TatI WGTACW 2 cut(s) 276, 590
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 3 cut(s) 8, 564, 689
Tru1I TTAA 2 cut(s) 27, 848
Tru9I TTAA 2 cut(s) 27, 848
TseFI GTSAC 2 cut(s) 90, 835
TseI GCWGC 3 cut(s) 233, 596, 892
Tsp45I GTSAC 2 cut(s) 90, 835
TspDTI ATGAA 3 cut(s) 522, 681, 773
TspGWI ACGGA 1 cut(s) 576
Tth111I GACNNNGTC 1 cut(s) 242
Vha464I CTTAAG 1 cut(s) 847
VpaK11BI GGWCC 1 cut(s) 550
XapI RAATTY 5 cut(s) 139, 269, 510, 656, 787
XhoI CTCGAG 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 384
XmnI GAANNNNTTC 1 cut(s) 617
XspI CTAG 3 cut(s) 170, 488, 734
ZrmI AGTACT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.