Rw2G025330

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001605
Physical Location & Seq
Reverse (-)
37844402 .. 37845745
1344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G025330.1

Sequence Viewer

Length: 1344 bp
ATGAATGAAACTCCAAGTCGGAGCATCACAATCCTTGAACAGTCATCTAAGGAGAAGTTGTTCAAAAACATAAAGGAAGCAGAAGAAAGGCTAGCTCAGCCTCAGTCTCAAAGCTCAAAACCAAAGATCCAAAGGGTTCCTGACATGCTCCAAGATCACCGAAAGTTTGAGAAATACTATGAGCCAAGGGCAGTAGCAATTGGTCCTTTCCATCATAATAAAGCAAGGTGTCAGCTGGCAGAGAAGTTCAAGCCTGCACTGGCAGCAAAATTTATCAAAGAAAGCGGCCGTCAAGCTGAAGATTTGCACAAGGTCATTGAGGACAACATCAGCACACTGAAGGACTGCTACAATGAGGAAGCAACAAGCTCCTACACCGACGAGGCCCTCACAGACTTGTTATTCTTAGATGGATGTTCAACCCTACAGTTCATATACTCCTTTATGCACCATGAGTTAGCAAGTTTCAAGATCAAGAGAGATCAGGTGGCTTTTGCAGAACAAGATTTGTTCTTGCTAGAAAACCAACTCCCTTACCAAGTCCTCGAGTTGTTGATGCAGAAAAGTGACAAACAGGCGGACTTGAAGACCGCAATCGATGATTTTGTTAAAGAGACAGCAGACAAAAATCCAAAAAGGCACCAACACAAAAGAACACGTCCACTAGGAGGTACATCCATAAACATGGAAGAGCCGCCCACTCATCTTTTAGAGCTTCTCCGAACAGAAATGCTAGGTCCCCCAAAATCTGACCGCCCCAAGAAGAAAAAACATAAAGATCGCCGACAATCATTCCGCAACATACAGGAGCTTAAAGCAGCAGGGATCCATTTAAAGCCTAGTAAGGGAAGAACCTTGAGGGACATATCTTTTACTTCTCTAGGCTTTCTAGGATTCCTAAATCTTCCACCAATATCGGTAGATGACTCCACGAGGCCCAAGTTCTTGAATTTGATAGCCTATGAGATGTGTCCTGATTTCCAAAATGACTTTGGGGTCACCTCTTACATATGCTTCCTGGATTCACTGATTGATCATGCAGATGATGTTAAACAGCTAAGGAAGGCACACATACTACACAACTTACTTGGGAGTGATGAAGAGGTGGCTAAACTTTTCAATAAGATAGGTACTGATTTGGTGCCTAACAGTGAAACATACCTCGGTGTCAAAGACGAATTAGAAAGGCACTACAAAAACATGTGGAAAACCTGGATGGCTCAATTCTGTCACGATCATTTCAGTAGCCCCTGGACTATCCTGGCTTTCCTTGGTGTTCTAGCAGCTCTAGGGTTAAGCGGCATCCAGACCTGGTACACAATCAATTCTCCCTCCTCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

447

Amino Acids

51.34

Weight (kDa)

7.68

Isoelectric Point (pI)

49.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 43 - 430 9.3e-96 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 995
AccB1I GGYRCC 2 cut(s) 639, 1141
AciI CCGC 7 cut(s) 285, 578, 591, 695, 754, 796, 1299
AclWI GGATC 3 cut(s) 121, 820, 833
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 2 cut(s) 269, 949
AcuI CTGAAG 2 cut(s) 318, 359
AfaI GTAC 3 cut(s) 673, 1132, 1316
AfiI CCNNNNNNNGG 2 cut(s) 668, 845
AflIII ACRYGT 2 cut(s) 656, 1200
AgsI TTSAA 8 cut(s) 38, 64, 250, 420, 469, 586, 949, 1120
AjiI CACGTC 1 cut(s) 659
AjnI CCWGG 5 cut(s) 1017, 1211, 1250, 1260, 1310
AloI GAACNNNNNNTCC 2 cut(s) 44, 76
AluBI AGCT 9 cut(s) 95, 114, 235, 296, 369, 715, 811, 1057, 1286
AluI AGCT 9 cut(s) 95, 114, 235, 296, 369, 715, 811, 1057, 1286
Alw26I GTCTC 2 cut(s) 111, 608
AlwI GGATC 3 cut(s) 121, 820, 833
Ama87I CYCGRG 1 cut(s) 545
AoxI GGCC 3 cut(s) 286, 384, 935
ApeKI GCWGC 3 cut(s) 263, 818, 1283
ApoI RAATTY 2 cut(s) 269, 949
Asp700I GAANNNNTTC 1 cut(s) 59
AspS9I GGNCC 4 cut(s) 203, 385, 737, 936
AsuHPI GGTGA 2 cut(s) 149, 991
AsuNHI GCTAGC 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 545
AvaII GGWCC 2 cut(s) 203, 737
BamHI GGATCC 1 cut(s) 825
BanI GGYRCC 2 cut(s) 639, 1141
BarI GAAGNNNNNNTAC 2 cut(s) 332, 364
BauI CACGAG 1 cut(s) 931
BbsI GAAGAC 1 cut(s) 593
BbvI GCAGC 3 cut(s) 275, 830, 1295
BccI CCATC 3 cut(s) 219, 404, 1210
BceAI ACGGC 1 cut(s) 273
BciT130I CCWGG 5 cut(s) 1019, 1213, 1252, 1262, 1312
BclI TGATCA 1 cut(s) 1033
BcoDI GTCTC 2 cut(s) 111, 608
BfaI CTAG 9 cut(s) 92, 518, 665, 734, 840, 881, 890, 1280, 1289
BfmI CTRYAG 1 cut(s) 425
BisI GCNGC 6 cut(s) 264, 286, 695, 819, 1284, 1300
BlpI GCTNAGC 1 cut(s) 96
BlsI GCNGC 6 cut(s) 265, 287, 696, 820, 1285, 1301
Bme1390I CCNGG 5 cut(s) 1019, 1213, 1252, 1262, 1312
Bme18I GGWCC 2 cut(s) 203, 737
BmeT110I CYCGRG 1 cut(s) 545
BmgBI CACGTC 1 cut(s) 659
BmgT120I GGNCC 4 cut(s) 203, 385, 737, 936
BmiI GGNNCC 5 cut(s) 138, 641, 739, 827, 1143
BmrFI CCNGG 5 cut(s) 1019, 1213, 1252, 1262, 1312
BmsI GCATC 3 cut(s) 33, 546, 1311
BmtI GCTAGC 1 cut(s) 95
BpiI GAAGAC 1 cut(s) 593
Bpu10I CCTNAGC 1 cut(s) 1058
Bpu1102I GCTNAGC 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 877
Bsa29I ATCGAT 1 cut(s) 597
BsaJI CCNNGG 4 cut(s) 185, 1162, 1250, 1270
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 2 cut(s) 668, 845
Bse1I ACTGG 1 cut(s) 264
BseBI CCWGG 5 cut(s) 1019, 1213, 1252, 1262, 1312
BseCI ATCGAT 1 cut(s) 597
BseDI CCNNGG 4 cut(s) 185, 1162, 1250, 1270
BseGI GGATG 4 cut(s) 419, 674, 1221, 1302
BseLI CCNNNNNNNGG 2 cut(s) 668, 845
BseMII CTCAG 2 cut(s) 110, 116
BseNI ACTGG 1 cut(s) 264
BseRI GAGGAG 1 cut(s) 1324
BseX3I CGGCCG 1 cut(s) 286
BseXI GCAGC 3 cut(s) 275, 830, 1295
BsgI GTGCAG 1 cut(s) 240
Bsh1285I CGRYCG 1 cut(s) 289
BshFI GGCC 3 cut(s) 288, 386, 937
BshNI GGYRCC 2 cut(s) 639, 1141
BshVI ATCGAT 1 cut(s) 597
BsiEI CGRYCG 1 cut(s) 289
BsiHKCI CYCGRG 1 cut(s) 545
BslFI GGGAC 2 cut(s) 723, 875
BslI CCNNNNNNNGG 2 cut(s) 668, 845
BsmAI GTCTC 2 cut(s) 111, 608
BsmFI GGGAC 2 cut(s) 723, 875
BsnI GGCC 3 cut(s) 288, 386, 937
BsoBI CYCGRG 1 cut(s) 545
Bsp143I GATC 8 cut(s) 126, 154, 471, 481, 778, 825, 1033, 1234
Bsp1720I GCTNAGC 1 cut(s) 96
BspACI CCGC 7 cut(s) 285, 578, 591, 695, 754, 796, 1299
BspANI GGCC 3 cut(s) 288, 386, 937
BspCNI CTCAG 2 cut(s) 109, 115
BspDI ATCGAT 1 cut(s) 597
BspLI GGNNCC 5 cut(s) 138, 641, 739, 827, 1143
BspOI GCTAGC 1 cut(s) 95
BspPI GGATC 3 cut(s) 121, 820, 833
BspQI GCTCTTC 1 cut(s) 684
BspT107I GGYRCC 2 cut(s) 639, 1141
BsrI ACTGG 1 cut(s) 264
BssECI CCNNGG 4 cut(s) 185, 1162, 1250, 1270
BssMI GATC 8 cut(s) 126, 154, 471, 481, 778, 825, 1033, 1234
BssSI CACGAG 1 cut(s) 931
BssT1I CCWWGG 2 cut(s) 185, 1270
Bst2BI CACGAG 1 cut(s) 931
Bst2UI CCWGG 5 cut(s) 1019, 1213, 1252, 1262, 1312
Bst4CI ACNGT 3 cut(s) 42, 429, 1151
Bst6I CTCTTC 2 cut(s) 684, 1095
BstC8I GCNNGC 3 cut(s) 93, 237, 255
BstDEI CTNAG 5 cut(s) 48, 96, 102, 406, 1058
BstEII GGTNACC 1 cut(s) 997
BstF5I GGATG 4 cut(s) 419, 674, 1221, 1302
BstKTI GATC 8 cut(s) 129, 157, 474, 484, 781, 828, 1036, 1237
BstMAI GTCTC 2 cut(s) 111, 608
BstMBI GATC 8 cut(s) 126, 154, 471, 481, 778, 825, 1033, 1234
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 2 cut(s) 97, 263
BstNI CCWGG 5 cut(s) 1019, 1213, 1252, 1262, 1312
BstNSI RCATGY 2 cut(s) 148, 1204
BstPI GGTNACC 1 cut(s) 997
BstSCI CCNGG 5 cut(s) 1017, 1211, 1250, 1260, 1310
BstSFI CTRYAG 1 cut(s) 425
BstV1I GCAGC 3 cut(s) 275, 830, 1295
BstV2I GAAGAC 1 cut(s) 593
BstX2I RGATCY 2 cut(s) 126, 825
BstXI CCANNNNNNTGG 1 cut(s) 685
BstYI RGATCY 2 cut(s) 126, 825
BstZI CGGCCG 1 cut(s) 286
Bsu15I ATCGAT 1 cut(s) 597
BsuRI GGCC 3 cut(s) 288, 386, 937
BsuTUI ATCGAT 1 cut(s) 597
BtrI CACGTC 1 cut(s) 659
BtsCI GGATG 4 cut(s) 419, 674, 1221, 1302
BtsIMutI CAGTG 4 cut(s) 257, 335, 1025, 1156
Cac8I GCNNGC 3 cut(s) 93, 237, 255
Cfr13I GGNCC 4 cut(s) 203, 385, 737, 936
ClaI ATCGAT 1 cut(s) 597
CsiI ACCWGGT 1 cut(s) 1310
Csp6I GTAC 3 cut(s) 672, 1131, 1315
CviAII CATG 5 cut(s) 145, 452, 685, 1037, 1201
CviQI GTAC 3 cut(s) 672, 1131, 1315
DdeI CTNAG 5 cut(s) 48, 96, 102, 406, 1058
DpnI GATC 8 cut(s) 128, 156, 473, 483, 780, 827, 1035, 1236
DpnII GATC 8 cut(s) 126, 154, 471, 481, 778, 825, 1033, 1234
DraI TTTAAA 1 cut(s) 834
DrdI GACNNNNNNGTC 1 cut(s) 995
DseDI GACNNNNNNGTC 1 cut(s) 995
EaeI YGGCCR 1 cut(s) 286
EagI CGGCCG 1 cut(s) 286
Eam1104I CTCTTC 2 cut(s) 684, 1095
EarI CTCTTC 2 cut(s) 684, 1095
EciI GGCGGA 1 cut(s) 593
EclXI CGGCCG 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 185, 1270
Eco47I GGWCC 2 cut(s) 203, 737
Eco52I CGGCCG 1 cut(s) 286
Eco57I CTGAAG 2 cut(s) 318, 359
Eco88I CYCGRG 1 cut(s) 545
Eco91I GGTNACC 1 cut(s) 997
EcoO109I RGGNCCY 2 cut(s) 385, 737
EcoO65I GGTNACC 1 cut(s) 997
EcoRII CCWGG 5 cut(s) 1017, 1211, 1250, 1260, 1310
EcoT14I CCWWGG 2 cut(s) 185, 1270
ErhI CCWWGG 2 cut(s) 185, 1270
FaeI CATG 5 cut(s) 148, 455, 688, 1040, 1204
FaqI GGGAC 2 cut(s) 723, 875
FatI CATG 5 cut(s) 144, 451, 684, 1036, 1200
FauNDI CATATG 1 cut(s) 1010
FbaI TGATCA 1 cut(s) 1033
Fnu4HI GCNGC 6 cut(s) 264, 286, 695, 819, 1284, 1300
FokI GGATG 4 cut(s) 426, 661, 1228, 1289
Fsp4HI GCNGC 6 cut(s) 264, 286, 695, 819, 1284, 1300
FspBI CTAG 9 cut(s) 92, 518, 665, 734, 840, 881, 890, 1280, 1289
GluI GCNGC 6 cut(s) 264, 286, 695, 819, 1284, 1300
HaeIII GGCC 3 cut(s) 288, 386, 937
Hin1II CATG 5 cut(s) 148, 455, 688, 1040, 1204
HinfI GANTC 3 cut(s) 894, 926, 1022
HphI GGTGA 2 cut(s) 149, 991
Hpy166II GTNNAC 2 cut(s) 662, 1317
Hpy188I TCNGA 3 cut(s) 21, 722, 751
Hpy188III TCNNGA 8 cut(s) 140, 469, 475, 946, 974, 1232, 1306, 1341
Hpy8I GTNNAC 2 cut(s) 662, 1317
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 2 cut(s) 334, 1057
HpyCH4III ACNGT 3 cut(s) 42, 429, 1151
HpyCH4IV ACGT 1 cut(s) 658
HpyCH4V TGCA 6 cut(s) 257, 307, 448, 497, 559, 1040
HpyF10VI GCNNNNNNNGC 2 cut(s) 97, 263
HpyF3I CTNAG 5 cut(s) 48, 96, 102, 406, 1058
HpySE526I ACGT 1 cut(s) 658
Hsp92II CATG 5 cut(s) 148, 455, 688, 1040, 1204
Ksp22I TGATCA 1 cut(s) 1033
Kzo9I GATC 8 cut(s) 126, 154, 471, 481, 778, 825, 1033, 1234
LguI GCTCTTC 1 cut(s) 684
LmnI GCTCC 4 cut(s) 21, 153, 374, 808
Lsp1109I GCAGC 3 cut(s) 275, 830, 1295
LweI GCATC 3 cut(s) 33, 546, 1311
MabI ACCWGGT 1 cut(s) 1310
MaeI CTAG 9 cut(s) 92, 518, 665, 734, 840, 881, 890, 1280, 1289
MaeII ACGT 1 cut(s) 658
MaeIII GTNAC 3 cut(s) 566, 997, 1229
MalI GATC 8 cut(s) 128, 156, 473, 483, 780, 827, 1035, 1236
MboI GATC 8 cut(s) 126, 154, 471, 481, 778, 825, 1033, 1234
MboII GAAGA 9 cut(s) 95, 311, 598, 701, 775, 861, 896, 1112, 1329
MfeI CAATTG 1 cut(s) 198
MflI RGATCY 2 cut(s) 126, 825
MluCI AATT 6 cut(s) 198, 269, 949, 1178, 1223, 1324
MlyI GAGTC 1 cut(s) 920
MroXI GAANNNNTTC 1 cut(s) 59
MseI TTAA 5 cut(s) 609, 813, 833, 1050, 1295
MslI CAYNNNNRTG 2 cut(s) 683, 1041
MspA1I CMGCKG 1 cut(s) 235
MspR9I CCNGG 5 cut(s) 1019, 1213, 1252, 1262, 1312
MunI CAATTG 1 cut(s) 198
MvaI CCWGG 5 cut(s) 1019, 1213, 1252, 1262, 1312
MwoI GCNNNNNNNGC 2 cut(s) 97, 263
NdeI CATATG 1 cut(s) 1010
NdeII GATC 8 cut(s) 126, 154, 471, 481, 778, 825, 1033, 1234
NheI GCTAGC 1 cut(s) 91
NlaIII CATG 5 cut(s) 148, 455, 688, 1040, 1204
NlaIV GGNNCC 5 cut(s) 138, 641, 739, 827, 1143
NmuCI GTSAC 3 cut(s) 566, 997, 1229
NspI RCATGY 2 cut(s) 148, 1204
PaeR7I CTCGAG 1 cut(s) 545
PciI ACATGT 1 cut(s) 1200
PciSI GCTCTTC 1 cut(s) 684
PdmI GAANNNNTTC 1 cut(s) 59
PfeI GAWTC 2 cut(s) 894, 1022
PfoI TCCNGGA 1 cut(s) 1017
PkrI GCNGC 6 cut(s) 265, 287, 696, 820, 1285, 1301
PleI GAGTC 1 cut(s) 920
PpsI GAGTC 1 cut(s) 920
PpuMI RGGWCCY 1 cut(s) 737
PscI ACATGT 1 cut(s) 1200
Psp5II RGGWCCY 1 cut(s) 737
Psp6I CCWGG 5 cut(s) 1017, 1211, 1250, 1260, 1310
PspEI GGTNACC 1 cut(s) 997
PspGI CCWGG 5 cut(s) 1017, 1211, 1250, 1260, 1310
PspN4I GGNNCC 5 cut(s) 138, 641, 739, 827, 1143
PspPI GGNCC 4 cut(s) 203, 385, 737, 936
PspPPI RGGWCCY 1 cut(s) 737
PspXI VCTCGAGB 1 cut(s) 545
PsuI RGATCY 2 cut(s) 126, 825
PvuII CAGCTG 1 cut(s) 235
RsaI GTAC 3 cut(s) 673, 1132, 1316
RsaNI GTAC 3 cut(s) 672, 1131, 1315
RseI CAYNNNNRTG 2 cut(s) 683, 1041
SapI GCTCTTC 1 cut(s) 684
SaqAI TTAA 5 cut(s) 609, 813, 833, 1050, 1295
SatI GCNGC 6 cut(s) 264, 286, 695, 819, 1284, 1300
Sau3AI GATC 8 cut(s) 126, 154, 471, 481, 778, 825, 1033, 1234
Sau96I GGNCC 4 cut(s) 203, 385, 737, 936
SchI GAGTC 1 cut(s) 920
ScrFI CCNGG 5 cut(s) 1019, 1213, 1252, 1262, 1312
SexAI ACCWGGT 1 cut(s) 1310
SfaNI GCATC 3 cut(s) 33, 546, 1311
SfcI CTRYAG 1 cut(s) 425
Sfr274I CTCGAG 1 cut(s) 545
SinI GGWCC 2 cut(s) 203, 737
SlaI CTCGAG 1 cut(s) 545
SmiMI CAYNNNNRTG 2 cut(s) 683, 1041
SmlI CTYRAG 2 cut(s) 545, 856
SmoI CTYRAG 2 cut(s) 545, 856
Sse9I AATT 6 cut(s) 198, 269, 949, 1178, 1223, 1324
SsiI CCGC 7 cut(s) 285, 578, 591, 695, 754, 796, 1299
SspMI CTAG 9 cut(s) 92, 518, 665, 734, 840, 881, 890, 1280, 1289
StyD4I CCNGG 5 cut(s) 1017, 1211, 1250, 1260, 1310
StyI CCWWGG 2 cut(s) 185, 1270
TaaI ACNGT 3 cut(s) 42, 429, 1151
TaiI ACGT 1 cut(s) 661
TaqI TCGA 2 cut(s) 546, 597
TasI AATT 6 cut(s) 198, 269, 949, 1178, 1223, 1324
TauI GCSGC 3 cut(s) 288, 697, 1302
TfiI GAWTC 2 cut(s) 894, 1022
Tru1I TTAA 5 cut(s) 609, 813, 833, 1050, 1295
Tru9I TTAA 5 cut(s) 609, 813, 833, 1050, 1295
TscAI CASTG 4 cut(s) 264, 342, 1032, 1156
TseFI GTSAC 3 cut(s) 566, 997, 1229
TseI GCWGC 3 cut(s) 263, 818, 1283
Tsp45I GTSAC 3 cut(s) 566, 997, 1229
TspDTI ATGAA 4 cut(s) 17, 21, 421, 1113
TspRI CASTG 4 cut(s) 264, 342, 1032, 1156
VpaK11BI GGWCC 2 cut(s) 203, 737
XapI RAATTY 2 cut(s) 269, 949
XceI RCATGY 2 cut(s) 148, 1204
XcmI CCANNNNNNNNNTGG 1 cut(s) 989
XhoI CTCGAG 1 cut(s) 545
XmnI GAANNNNTTC 1 cut(s) 59
XspI CTAG 9 cut(s) 92, 518, 665, 734, 840, 881, 890, 1280, 1289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.