Prupe.7G045600_v2.0.a1

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001605
Physical Location & Seq
Forward (+)
8150420 .. 8152173
1754 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G045600.1

Sequence Viewer

Length: 1155 bp
ATGAGATTGTCCAATAAAGGATTTGGTATACGAGTTGATAAGATGCGTCTCTTCCAATGCGCTAAAGCAGCTAGAAGCTTAATACCAAAGATCCAAAGTGTTCCCAGAATTCTCCGAAGTCACAGAAATTCAGAGAAGTACTTTGAGCCAAGGGTAGTGGCAATGACCCCTATTTACACCATAGTGAACCGAGCATATCCAGCACCAAAGCGACTAGCACCAAGGCAGCCACGCCTTCTTCCCTGCCGAAGGAAGACACACTTCCGAGCGCCAACAGCCCCTATCAATCAATGCAAGTACAAAGAAGCAGATGAACTCAAATACATATTGGCAGCAGACTTTATCCATGATAGCGGCCAGAAAGATGAAGATTTGTACAAGAAAATTAAGGACTGCATCAAGCTTATAAAGGACTGCTACGATATGGAAACAACAAAGTGCTATGATGATGATGAGATATTGTTCATAGATGGATGTTCAACTTTGCAATTTATATATAAACGTCATTGCTTGGAAGAGTATGGGATGAAGAGAGACCAAGAGGCCTTCGCAGAGCAGGACTTGTTCCTGCCAGAGAACCAACTCCCATATCAAGAGTTGAGTGCAATGGAGAGGTTTGCTCTGTGGCAGATTACTGCAGCAGAAGAGCCACCGGATGAGTACAAAAGGGGGTTTCAACAACGTGAAGGTCGACCAATCAACATGGGAGAAGAGCCACAGCCCGCTCATCTTCCTGATCTTCTTCGGAAAAGACTGCTAGGTCCAGCACTTACTCACATGGAGAAGTGGATTAGCACTGAGGATCGTTTCCCATTGTACCACAATGCCCAGGAGCTTAAGGCAGCCAGGACCCATTTCGGGCGTGGCAAGGGATTTCTTAGTCTTCCTCCCATAAACGAAGACGCGATGTCTTTGTTCTTGAATTTGATAGCCTACGAAATGTGTCCTGATTTCCTAAATGATTTTGTGGTCACCTTTTTCTTCGGCTTCCCCAGTTCAGTCGTTGCTCATCCCGACGATGCTAAGCAGCTACAGTCTGCCCGCATATTTTGCAACTTGCGTGGGAGTGATGAAGCCCTCGCTGACCTCTTTCATGAGATAGGCCCTGATTTGGCGTCTACCCATTCTATACACAACATTGTCAGTGCCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

385

Amino Acids

44.41

Weight (kDa)

7.17

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 407
AasI GACNNNNNNGTC 1 cut(s) 759
AccBSI CCGCTC 1 cut(s) 725
AccI GTMKAC 3 cut(s) 28, 691, 1118
AccII CGCG 1 cut(s) 905
AciI CCGC 3 cut(s) 354, 723, 1042
AclWI GGATC 2 cut(s) 85, 810
AcoI YGGCCR 1 cut(s) 355
AcsI RAATTY 3 cut(s) 108, 127, 922
AcyI GRCGYC 1 cut(s) 1115
AfaI GTAC 5 cut(s) 140, 299, 377, 662, 818
AfiI CCNNNNNNNGG 3 cut(s) 249, 858, 1111
AflII CTTAAG 1 cut(s) 836
AgsI TTSAA 3 cut(s) 480, 677, 922
AhdI GACNNNNNGTC 1 cut(s) 907
AjnI CCWGG 2 cut(s) 828, 845
AleI CACNNNNGTG 1 cut(s) 182
AluBI AGCT 5 cut(s) 71, 78, 403, 835, 1030
AluI AGCT 5 cut(s) 71, 78, 403, 835, 1030
Alw26I GTCTC 2 cut(s) 53, 528
AlwI GGATC 2 cut(s) 85, 810
AoxI GGCC 3 cut(s) 355, 543, 1102
ApeKI GCWGC 6 cut(s) 68, 226, 332, 638, 842, 1027
ApoI RAATTY 3 cut(s) 108, 127, 922
AspLEI GCGC 2 cut(s) 62, 271
AspS9I GGNCC 3 cut(s) 761, 849, 1103
AsuHPI GGTGA 1 cut(s) 964
AvaII GGWCC 2 cut(s) 761, 849
BbsI GAAGAC 3 cut(s) 260, 875, 906
BbvI GCAGC 6 cut(s) 80, 238, 344, 650, 854, 1039
BccI CCATC 1 cut(s) 464
BciT130I CCWGG 2 cut(s) 830, 847
BcoDI GTCTC 2 cut(s) 53, 528
BfaI CTAG 3 cut(s) 72, 215, 758
BfmI CTRYAG 2 cut(s) 636, 1031
BfoI RGCGCY 1 cut(s) 272
BfrI CTTAAG 1 cut(s) 836
BisI GCNGC 7 cut(s) 69, 227, 333, 355, 639, 843, 1028
BlpI GCTNAGC 1 cut(s) 1023
BlsI GCNGC 7 cut(s) 70, 228, 334, 356, 640, 844, 1029
BmcAI AGTACT 1 cut(s) 140
Bme1390I CCNGG 2 cut(s) 830, 847
Bme18I GGWCC 2 cut(s) 761, 849
BmeRI GACNNNNNGTC 1 cut(s) 907
BmgT120I GGNCC 3 cut(s) 761, 849, 1103
BmiI GGNNCC 1 cut(s) 851
BmrFI CCNGG 2 cut(s) 830, 847
BmrI ACTGGG 1 cut(s) 987
BmsI GCATC 3 cut(s) 33, 405, 1009
BmuI ACTGGG 1 cut(s) 987
BpiI GAAGAC 3 cut(s) 260, 875, 906
BplI GAGNNNNNCTC 2 cut(s) 604, 636
Bpu1102I GCTNAGC 1 cut(s) 1023
BsaHI GRCGYC 1 cut(s) 1115
BsaI GGTCTC 1 cut(s) 528
BsaJI CCNNGG 3 cut(s) 149, 221, 828
BsaWI WCCGGW 1 cut(s) 652
Bsc4I CCNNNNNNNGG 3 cut(s) 249, 858, 1111
Bse1I ACTGG 1 cut(s) 993
Bse3DI GCAATG 3 cut(s) 168, 505, 612
BseBI CCWGG 2 cut(s) 830, 847
BseDI CCNNGG 3 cut(s) 149, 221, 828
BseGI GGATG 4 cut(s) 479, 531, 661, 1009
BseLI CCNNNNNNNGG 3 cut(s) 249, 858, 1111
BseMI GCAATG 3 cut(s) 168, 505, 612
BseMII CTCAG 1 cut(s) 789
BseNI ACTGG 1 cut(s) 993
BseXI GCAGC 6 cut(s) 80, 238, 344, 650, 854, 1039
Bsh1236I CGCG 1 cut(s) 905
BshFI GGCC 3 cut(s) 357, 545, 1104
BsiSI CCGG 1 cut(s) 653
BslI CCNNNNNNNGG 3 cut(s) 249, 858, 1111
BsmAI GTCTC 2 cut(s) 53, 528
BsmBI CGTCTC 1 cut(s) 53
BsnI GGCC 3 cut(s) 357, 545, 1104
Bso31I GGTCTC 1 cut(s) 528
Bsp1407I TGTACA 1 cut(s) 375
Bsp143I GATC 3 cut(s) 90, 736, 802
Bsp1720I GCTNAGC 1 cut(s) 1023
BspACI CCGC 3 cut(s) 354, 723, 1042
BspANI GGCC 3 cut(s) 357, 545, 1104
BspCNI CTCAG 1 cut(s) 790
BspFNI CGCG 1 cut(s) 905
BspHI TCATGA 1 cut(s) 1093
BspLI GGNNCC 1 cut(s) 851
BspMAI CTGCAG 1 cut(s) 640
BspPI GGATC 2 cut(s) 85, 810
BspQI GCTCTTC 2 cut(s) 639, 705
BspTI CTTAAG 1 cut(s) 836
BspTNI GGTCTC 1 cut(s) 528
BsrBI CCGCTC 1 cut(s) 725
BsrDI GCAATG 3 cut(s) 168, 505, 612
BsrGI TGTACA 1 cut(s) 375
BsrI ACTGG 1 cut(s) 993
BssECI CCNNGG 3 cut(s) 149, 221, 828
BssMI GATC 3 cut(s) 90, 736, 802
BssNAI GTATAC 1 cut(s) 29
BssNI GRCGYC 1 cut(s) 1115
BssT1I CCWWGG 2 cut(s) 149, 221
Bst1107I GTATAC 1 cut(s) 29
Bst2UI CCWGG 2 cut(s) 830, 847
Bst4CI ACNGT 1 cut(s) 1035
Bst6I CTCTTC 5 cut(s) 56, 510, 524, 639, 705
BstACI GRCGYC 1 cut(s) 1115
BstAFI CTTAAG 1 cut(s) 836
BstAPI GCANNNNNTGC 1 cut(s) 1050
BstAUI TGTACA 1 cut(s) 375
BstC8I GCNNGC 2 cut(s) 723, 1042
BstDEI CTNAG 3 cut(s) 798, 878, 1023
BstEII GGTNACC 1 cut(s) 970
BstENI CCTNNNNNAGG 1 cut(s) 247
BstF5I GGATG 4 cut(s) 479, 531, 661, 1009
BstFNI CGCG 1 cut(s) 905
BstH2I RGCGCY 1 cut(s) 272
BstHHI GCGC 2 cut(s) 62, 271
BstKTI GATC 3 cut(s) 93, 739, 805
BstMAI GTCTC 2 cut(s) 53, 528
BstMBI GATC 3 cut(s) 90, 736, 802
BstMWI GCNNNNNNNGC 4 cut(s) 68, 200, 275, 1050
BstNI CCWGG 2 cut(s) 830, 847
BstPI GGTNACC 1 cut(s) 970
BstSCI CCNGG 2 cut(s) 828, 845
BstSFI CTRYAG 2 cut(s) 636, 1031
BstUI CGCG 1 cut(s) 905
BstV1I GCAGC 6 cut(s) 80, 238, 344, 650, 854, 1039
BstV2I GAAGAC 3 cut(s) 260, 875, 906
BstX2I RGATCY 1 cut(s) 90
BstYI RGATCY 1 cut(s) 90
BstZ17I GTATAC 1 cut(s) 29
BsuRI GGCC 3 cut(s) 357, 545, 1104
BtgZI GCGATG 1 cut(s) 920
BtsCI GGATG 4 cut(s) 479, 531, 661, 1009
BtsIMutI CAGTG 2 cut(s) 795, 1150
Cac8I GCNNGC 2 cut(s) 723, 1042
CciI TCATGA 1 cut(s) 1093
CfoI GCGC 2 cut(s) 62, 271
Cfr13I GGNCC 3 cut(s) 761, 849, 1103
CseI GACGC 3 cut(s) 35, 911, 1104
Csp6I GTAC 5 cut(s) 139, 298, 376, 661, 817
CspCI CAANNNNNGTGG 4 cut(s) 138, 173, 1042, 1077
CviAII CATG 4 cut(s) 347, 703, 778, 1094
CviQI GTAC 5 cut(s) 139, 298, 376, 661, 817
DdeI CTNAG 3 cut(s) 798, 878, 1023
DpnI GATC 3 cut(s) 92, 738, 804
DpnII GATC 3 cut(s) 90, 736, 802
DrdI GACNNNNNNGTC 1 cut(s) 759
DriI GACNNNNNGTC 1 cut(s) 907
DseDI GACNNNNNNGTC 1 cut(s) 759
EaeI YGGCCR 1 cut(s) 355
Eam1104I CTCTTC 5 cut(s) 56, 510, 524, 639, 705
Eam1105I GACNNNNNGTC 1 cut(s) 907
EarI CTCTTC 5 cut(s) 56, 510, 524, 639, 705
Eco130I CCWWGG 2 cut(s) 149, 221
Eco147I AGGCCT 1 cut(s) 545
Eco31I GGTCTC 1 cut(s) 528
Eco47I GGWCC 2 cut(s) 761, 849
Eco91I GGTNACC 1 cut(s) 970
EcoNI CCTNNNNNAGG 1 cut(s) 247
EcoO109I RGGNCCY 2 cut(s) 849, 1103
EcoO65I GGTNACC 1 cut(s) 970
EcoRI GAATTC 1 cut(s) 108
EcoRII CCWGG 2 cut(s) 828, 845
EcoT14I CCWWGG 2 cut(s) 149, 221
ErhI CCWWGG 2 cut(s) 149, 221
Esp3I CGTCTC 1 cut(s) 53
FaeI CATG 4 cut(s) 350, 706, 781, 1097
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FatI CATG 4 cut(s) 346, 702, 777, 1093
FauI CCCGC 2 cut(s) 730, 1049
FblI GTMKAC 3 cut(s) 28, 691, 1118
Fnu4HI GCNGC 7 cut(s) 69, 227, 333, 355, 639, 843, 1028
FokI GGATG 4 cut(s) 486, 538, 668, 996
Fsp4HI GCNGC 7 cut(s) 69, 227, 333, 355, 639, 843, 1028
FspBI CTAG 3 cut(s) 72, 215, 758
GlaI GCGC 2 cut(s) 61, 270
GluI GCNGC 7 cut(s) 69, 227, 333, 355, 639, 843, 1028
HaeII RGCGCY 1 cut(s) 272
HaeIII GGCC 3 cut(s) 357, 545, 1104
HapII CCGG 1 cut(s) 653
HgaI GACGC 3 cut(s) 35, 911, 1104
HhaI GCGC 2 cut(s) 62, 271
Hin1I GRCGYC 1 cut(s) 1115
Hin1II CATG 4 cut(s) 350, 706, 781, 1097
Hin6I GCGC 2 cut(s) 60, 269
HinP1I GCGC 2 cut(s) 60, 269
HincII GTYRAC 1 cut(s) 692
HindII GTYRAC 1 cut(s) 692
HindIII AAGCTT 2 cut(s) 76, 401
HpaII CCGG 1 cut(s) 653
HphI GGTGA 1 cut(s) 964
Hpy166II GTNNAC 4 cut(s) 29, 187, 692, 1119
Hpy188I TCNGA 4 cut(s) 116, 133, 266, 747
Hpy188III TCNNGA 6 cut(s) 593, 734, 919, 947, 1013, 1094
Hpy8I GTNNAC 4 cut(s) 29, 187, 692, 1119
Hpy99I CGWCG 1 cut(s) 1019
HpyAV CCTTC 4 cut(s) 243, 245, 556, 680
HpyCH4III ACNGT 1 cut(s) 1035
HpyCH4IV ACGT 2 cut(s) 502, 682
HpyCH4V TGCA 6 cut(s) 294, 396, 487, 605, 638, 1053
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 200, 275, 1050
HpyF3I CTNAG 3 cut(s) 798, 878, 1023
HpySE526I ACGT 2 cut(s) 502, 682
Hsp92I GRCGYC 1 cut(s) 1115
Hsp92II CATG 4 cut(s) 350, 706, 781, 1097
HspAI GCGC 2 cut(s) 60, 269
Kzo9I GATC 3 cut(s) 90, 736, 802
LguI GCTCTTC 2 cut(s) 639, 705
LmnI GCTCC 1 cut(s) 832
Lsp1109I GCAGC 6 cut(s) 80, 238, 344, 650, 854, 1039
LweI GCATC 3 cut(s) 33, 405, 1009
MaeI CTAG 3 cut(s) 72, 215, 758
MaeII ACGT 2 cut(s) 502, 682
MaeIII GTNAC 2 cut(s) 119, 970
MalI GATC 3 cut(s) 92, 738, 804
MbiI CCGCTC 1 cut(s) 725
MboI GATC 3 cut(s) 90, 736, 802
MflI RGATCY 1 cut(s) 90
MluCI AATT 6 cut(s) 108, 127, 384, 488, 922, 1150
MnlI CCTC 6 cut(s) 535, 606, 793, 897, 1088, 1097
MseI TTAA 3 cut(s) 80, 387, 837
MslI CAYNNNNRTG 1 cut(s) 182
MspCI CTTAAG 1 cut(s) 836
MspI CCGG 1 cut(s) 653
MspR9I CCNGG 2 cut(s) 830, 847
MvaI CCWGG 2 cut(s) 830, 847
MvnI CGCG 1 cut(s) 905
MwoI GCNNNNNNNGC 4 cut(s) 68, 200, 275, 1050
NdeII GATC 3 cut(s) 90, 736, 802
NlaIII CATG 4 cut(s) 350, 706, 781, 1097
NlaIV GGNNCC 1 cut(s) 851
NmuCI GTSAC 2 cut(s) 119, 970
OliI CACNNNNGTG 1 cut(s) 182
PagI TCATGA 1 cut(s) 1093
PceI AGGCCT 1 cut(s) 545
PciSI GCTCTTC 2 cut(s) 639, 705
PcsI WCGNNNNNNNCGW 1 cut(s) 688
PkrI GCNGC 7 cut(s) 70, 228, 334, 356, 640, 844, 1029
PpuMI RGGWCCY 1 cut(s) 849
PsiI TTATAA 1 cut(s) 407
Psp5II RGGWCCY 1 cut(s) 849
Psp6I CCWGG 2 cut(s) 828, 845
PspEI GGTNACC 1 cut(s) 970
PspGI CCWGG 2 cut(s) 828, 845
PspN4I GGNNCC 1 cut(s) 851
PspPI GGNCC 3 cut(s) 761, 849, 1103
PspPPI RGGWCCY 1 cut(s) 849
PstI CTGCAG 1 cut(s) 640
PsuI RGATCY 1 cut(s) 90
RsaI GTAC 5 cut(s) 140, 299, 377, 662, 818
RsaNI GTAC 5 cut(s) 139, 298, 376, 661, 817
RseI CAYNNNNRTG 1 cut(s) 182
SalI GTCGAC 1 cut(s) 690
SapI GCTCTTC 2 cut(s) 639, 705
SaqAI TTAA 3 cut(s) 80, 387, 837
SatI GCNGC 7 cut(s) 69, 227, 333, 355, 639, 843, 1028
Sau3AI GATC 3 cut(s) 90, 736, 802
Sau96I GGNCC 3 cut(s) 761, 849, 1103
ScaI AGTACT 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 830, 847
SfaNI GCATC 3 cut(s) 33, 405, 1009
SfcI CTRYAG 2 cut(s) 636, 1031
SinI GGWCC 2 cut(s) 761, 849
SmiMI CAYNNNNRTG 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 836
SmoI CTYRAG 1 cut(s) 836
Sse9I AATT 6 cut(s) 108, 127, 384, 488, 922, 1150
SseBI AGGCCT 1 cut(s) 545
SsiI CCGC 3 cut(s) 354, 723, 1042
SspMI CTAG 3 cut(s) 72, 215, 758
StuI AGGCCT 1 cut(s) 545
StyD4I CCNGG 2 cut(s) 828, 845
StyI CCWWGG 2 cut(s) 149, 221
TaaI ACNGT 1 cut(s) 1035
TaiI ACGT 2 cut(s) 505, 685
TaqI TCGA 1 cut(s) 691
TasI AATT 6 cut(s) 108, 127, 384, 488, 922, 1150
TatI WGTACW 4 cut(s) 138, 297, 375, 660
TauI GCSGC 1 cut(s) 357
Tru1I TTAA 3 cut(s) 80, 387, 837
Tru9I TTAA 3 cut(s) 80, 387, 837
TscAI CASTG 2 cut(s) 802, 1150
TseFI GTSAC 2 cut(s) 119, 970
TseI GCWGC 6 cut(s) 68, 226, 332, 638, 842, 1027
Tsp45I GTSAC 2 cut(s) 119, 970
TspDTI ATGAA 6 cut(s) 327, 381, 454, 542, 1082, 1086
TspRI CASTG 2 cut(s) 802, 1150
Vha464I CTTAAG 1 cut(s) 836
VpaK11BI GGWCC 2 cut(s) 761, 849
XagI CCTNNNNNAGG 1 cut(s) 247
XapI RAATTY 3 cut(s) 108, 127, 922
XcmI CCANNNNNNNNNTGG 1 cut(s) 860
XmiI GTMKAC 3 cut(s) 28, 691, 1118
XspI CTAG 3 cut(s) 72, 215, 758
ZrmI AGTACT 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.